1T& R.AR.Y OF THE UNIVERSITY OF ILLINOIS' G30.7 UGb cop AGRICULTURE NOTICE: Return or renew all Library Materials! The Minimum Fee for each Lost Book is $50.00. The person charging this material is responsible for its return to the library from which it was wuhdrawn on or before the Latest Date stamped below. Theft, mutilation, and underlining of books are reasons for discipli- nary action and may result in dismissal from the University. To renew call Telephone Center, 333-8400 UNIVERSITY OF ILLINOIS LIBRARY AT URBANA-CHAMPAIGN L161 O-1096 \ UNIVERSITY OF ILLINOIS Agricultural Experiment Station BULLETIN NO. 279 THE TOXICITY OF SALT FOR CHICKENS By H. H. MITCHELL, L. E. CARD, AND G. G. CARMAN The presence of small amounts of salt in various feeds for poultry is not so dangerous as has commonly been believed. This bird was raised on a ration containing 8 percent of salt. URBANA, ILLINOIS, JULY, 1926 SUMMARY Because of the general belief that chickens are very readily pois- oned by common table salt, and because of the common occurrence of salt in wastes and by-product feeds for poultry, it was considered im- portant to determine definitely whether the use of such wastes and feeds is dangerous to health or retards growth. This experiment was planned, therefore, in order to determine (1) the maximum percentage of salt that may be fed to growing chickens without harmful results, and (2) the maximum single dose of salt that a chicken can tolerate, or the smallest dose that would cause death. In all, 75 chickens from three breeds were used. Fifty chickens were given a basal ration made up of yellow corn, bran, dried butter- milk, steamed bone meal, and ground limestone, containing percent- ages of salt of 1, 2, 4, and 8. Twenty-five other chickens were used in determining the maximum single dose of salt that an adult chicken can tolerate. It was found that chickens could be raised from 9 to 21 weeks of age on rations containing as high as 8 percent of salt with no appar- ently detrimental effects. While it took some time for the chickens to become accustomed to such a salty ration, they soon learned to eat it in amounts sufficient to promote a rate of growth approximately the same as that of chickens fed the check ration. When the salt was mixed in the feed, a daily intake of 6 to 8 grams of salt per bird appeared to have no harmful effect on the birds that were 9 weeks old or older. Salt put directly into the crop in two equal doses amounting to 12 to 16 grams daily was quickly fatal in the case of birds weighing 2 to 4 pounds each. Salt given in solution twice daily proved to be more toxic than equal amounts consumed in the feed. The minimum lethal single dose of salt for birds weighing from 3 to 5 pounds was found to be close to 4 grams per kilogram of body weight. By H. H. MITCHELL, L. E. CARD, AND G. G. CARMAN' It is a general belief among poultrymen that chickens are very readily poisoned by common table salt, and many instances have been reported in which chickens were killed by eating salt meat or fish, salty kitchen wastes, or brine left from the curing of meat or from the freezing of ice cream. It has been recommended by some writers that the proportion of salt in a mash for chickens be no higher than 5 to 10 ounces to 100 pounds of mash when the mash mixture comprizes about half the total ration, and that no salt be given to young stock until they are two months old. However, only a few controlled experiments concerned with a determination of the minimum lethal dose of salt for chickens have been reported in the literature. In 1892 Collier 2 reported the results of feeding varying amounts of salt to mature hens. No harmful results were noticed in these experi- ments involving six hens until the intake of salt reached 0.063 ounce per head per day. An intake of 0.042 ounce per head per day was not accompanied by any noticeable symptoms. In 1909 Suffran 3 attempted to determine the minimum toxic dose of common salt for chickens. From experiments on 5 chickens, he concluded that a dose of 4 grams per kilogram of body weight is sufficient to produce death, tho one chicken in the experiment resisted this dose successfully. Salt was ad- ministered in solution and was injected into the crop after the meal. The symptoms noticed were inability to stand, intense thirst, pro- nounced muscular weakness, and convulsive movements just before death. A viscous discharge from the beak was also noted. Post- mortem examination revealed lesions in many organs, but particularly hemorrhages and a severe congestion in the gastro-intestinal tract. The attention of Edwards 4 was directed toward the problem by a case in which several ducks, pigs, and chickens were killed by being fed the sweepings from the floor of a bakery, afterward found to con- tain about 22 percent of salt. The symptoms noted in the chickens were very similar to those above reported. An experiment was then 1 H. H. Mitchell, Chief in Animal Nutrition; L. E. Card, Chief in Poultry Husbandry; G. G. Carman, formerly Assistant in Animal Nutrition. 'Collier, Peter. N. Y. Agr. Exp. Sta. (Geneva), Bui. 39, N. S. 1892. 'Suffran, F. Rev. Gen. Med. Vet. 13, No. 156, 698-705. 1909. Exp. Sta. Rec. 23, 793. 1910. 'Edwards, J. T. Jour. Compar. Path, and Ther. 31, 40-43. 1918. 135 136 BULLETIN No. 279 [.July, performed upon three pigeons. Solutions containing 0.625, 1.25, and 2.50 grams of salt per kilogram of body weight, respectively, were in- jected into the crops of the birds. No ill effects from these doses were noted, except a temporary depression of the two birds given the largest doses. Five days later the same birds received doses amounting to 2.50, 3.33, and 4.50 grams per kilogram of body weight. The bird re- ceiving the highest dose died in 18 hours, the bird receiving the next highest dose died in 23 hours, while the one receiving the smallest dose showed no symptoms. Other experiments concerned with the symptoms of salt poisoning have been performed, 1 but in these experiments no attempt was made to determine the smallest toxic dose of salt. They need not, therefore, be reviewed here. The evidence bearing on the toxicity of salt for chickens is meager and somewhat contradictory. Because of the common occurrence of salt in domestic and industrial wastes and by-product feeds that are occasionally or regularly fed to poultry, it is of importance to deter- mine definitely whether the use of these wastes and feeds is a constant source of danger to the poultry flock. The experiments reported in this bulletin were undertaken, therefore, with two objects in view: first, to determine the effect of varying percentages of salt in the ration on the rate of growth of chickens; and second, to determine the small- est dose that would cause death. OUTLINE OF EXPERIMENT Five lots of 10 chicks each at the poultry farm and five lots of 5 chicks each at the nutrition laboratory were fed a well-balanced basal ration containing varying amounts of salt. Lot 1 at both the poultry plant and the nutrition laboratory received 1 percent of salt in their ration; Lot 2, 2 percent; Lot 3, 4 percent; Lot 4, 8 percent; and Lot 5, 1 percent with free access to rock salt. The lots fed at the poultry farm were kept in shelters open to the south and had the run of lots free from vegetation (Fig. 1). They were group-fed and were weighed individually each week. Feed records were kept on all lots. The chickens grown in the laboratory were confined in individual pens having a floor space of 4 square feet. They were fed individu- ally and were weighed weekly. All lots had free access to feed at all times, and the chickens fed individually at the nutrition laboratory were also given as much feed as they would consume. It was hoped that all chickens in the experi- ment could be selected from the same breed of birds at the same age, but unfortunately this ideal could not be realized. Each lot kept at 'Heinz and Haas. Munchen. Med. Wchnschr. 70, 565-66. 1923. 1926] THE TOXICITY OF SALT FOR CHICKENS 137 the poultry farm contained 5 White Wyandotte chickens and 5 White Leghorn chickens. The chickens grown at the nutrition laboratory were all Rhode Island Reds. The initial age of all chickens was 8 weeks. In the distribution of the birds among the several lots, the number of cockerels and pullets in each lot was made the same. FIG. 1. EXPERIMENTAL FEEDING PENS AT THE POULTRY PLANT USED FOR THE VARIOUS LOTS Chicks fed at the poultry farm were Jtept in shelters open to the south and had the run of the lots, which were free from vegetation. The feeding experiment at the poultry farm was continued for 13 weeks and that at the nutrition laboratory for 12 weeks. At the end of this time the birds were given salt either in capsules or in solution, in order to determine the minimum amount they could tolerate in re- peated doses and the minimum single toxic dose. EXPERIMENTAL RESULTS The rations used at the poultry farm and at the nutrition labora- tory are given in Table 1. The lots at the farm and at the laboratory receiving equal percentages of salt will be referred to by the same numbers. Lot 5 differed from Lot 1 in having access to salt at all times besides receiving 1 percent in its feed. It was found, however, that these chickens did not consume any of the salt offered to them, or if any was consumed it was so small an amount as to be obscured by the changes in moisture content of the salt offered. The salt intake of Lots 1 and 5 was therefore the same. 138 BULLETIN No. 279 [.July, For the first week of the experiment all lots were fed the ration containing 1 percent salt. On June 26, 1925, the lots were put upon the TABLE 1. BASAL RATION AND VARIATIONS IN SALT CONTENT FOR VARIOUS LOTS OF CHICKENS Feed Lots 1 and 5 Lot 2 Lot3 Lot 4 Corn 60 59 57 53 Wheat bran 15 15 15 15 Dried buttermilk 15 15 15 15 Steamed bone meal 5 5 5 5 Calcium carbonate (CaCOs) 4 4 4 4 Common salt (NaCl) 1 2 4 8 Ninety-eight parts of the above mixture was in each case mixed with 2 parts of cod-liver oil. different experimental rations and kept under observation for 12 weeks in the case of the birds at the poultry plant, and for 11 weeks in the case of the birds in the nutrition laboratory. EFFECT OF SALT ON GROWTH The rate of growth of the White Wyandotte chickens from June 19 is shown graphically in Fig. 2, the growth of the White Leghorns in Fig. 3, and the growth of the Rhode Island Red chickens in Fig. 4. Among the lots kept at the poultry farm, the growth of Lots 1, 2, 3, and 5 was very similar. Lot 4, receiving 8 percent of salt in its ration, lagged somewhat behind the other lots in its rate of growth, altho this is mainly due to a retardation of growth in the initial weeks of the experiment because of the slow adjustment of the chickens to the extremely salty ration. When the feed intake approximated that of the other groups, the rate of growth was about the same as that of the chickens getting smaller percentages of salt. There was some mortality among the lots kept at the poultry farm, but the cause of death seemed to be unrelated to the method of feeding. In Lots 1, 2, and 3 there were no deaths, in Lot 4 one White Wyandotte chicken died one week after experimental feeding started, and in Lot 5 two White Leghorn chickens died 11 and 32 days, re- spectively, after experimental feeding started. No abnormal symp- toms referable to the salt intake were observed in any of the groups. Group photographs of Lots 1 and 4 getting, respectively, 1 and 8 per- cent of salt in their feed, are shown in Figs. 5 and 6. No differences in the condition of the two groups of birds can be seen from a com- parison of these photographs. Fig. 7 shows two of the individual birds in Lot 4, one a White Leghorn and the other a White Wyandotte, 1 'Most of the White Wyandottes used in this experiment, including the one shown in Fig. 4, had single combs. 1926] THE TOXICITT OF SALT FOR CHICKENS 139 TKe GrowtK of WKite Wyandotte Chickens on Rations Differing Only in Their Content" of So.lt Lot I I percent salt Lot Z 2 " " Lot i 4 " Let 48"" Let 5 I The GrowtK of Whute LegNorrx GKicker\s or\ RatioNs Differmg Or\ly ir\ TKeir Cor\Ter\t of Salt Fig. 4 OrowtK of RKode Islorxd F\ed CMckerxs or\ Ratior\s Differing Only ir\ TKeir Content of Salt 140 BULLETIN No. 279 [July, FIG. 5. LOT 1 AFTER THIRTEEN WEEKS ON A RATION CONTAINING 1 PERCENT OF SALT No difference could be observed in the condition of the birds in Lot 1, which received 1 percent of salt, and Lot 4, which re- ceived 8 percent (see Fig. 6). FIG. 6. LOT 4 AFTER THIRTEEN WEEKS ON A RATION CONTAINING 8 PERCENT OF SALT The chickens in this lot lagged behind those in the other lots in rate of growth, owing chiefly to retardation of growth in the first weeks of the experiment before they became adjusted to the extremely salty ration. When their feed intake approximated that of the other groups, the rate of growth was about the same as for the chickens receiving less salt. 1926] THE TOXICITY OF SALT FOR CHICKENS 141 taken at the end of the feeding test. The chickens in Lot 4, particu- larly, drank and excreted large amounts of water, this fact accounting for their untidy appearance. The relative growth observed among the five lots of Rhode Island Red chickens kept at the laboratory was very similar to that of the birds kept at the poultry farm. The growth in Lot 4 again was some- what slower than that in the lots getting smaller percentages of salt, but after the birds became accustomed to the highly salty ration, the growth in Lot 4 was quite as rapid as that in the other lots ; in fact it FIG. 7. INDIVIDUAL BIRDS FROM LOT 4 FED A RATION CONTAINING 8 PERCENT OF SALT Even this extremely salty ration did not prove to be poisonous to chickens that were eight weeks old when the experiment was started. It was observed, however, that the chickens in this lot drank and ex- creted large amounts of water. was somewhat better than that observed in Lot 3, which received 4 percent of salt. The mortality in these lots also seemed to bear no relation to the method of feeding. One chicken in Lot 1 died after 6 weeks of feeding, and 2 chickens in Lot 3 after 7 weeks of feeding. Continued growth in the lots kept in the nutrition laboratory was prevented by the appearance of leg weakness among many of the birds. Whether this was due to the small percentage of cod-liver oil in the rations or to the confinement of the birds in such small pens cannot be deter- mined. 1 It was entirely unrelated to the salt content of the diet. The feeding experiment in the laboratory was discontinued one week sooner 'In an attempt to determine whether the leg weakness observed was due to rickets the tibias and fibulas from each of 7 affected chickens and 5 unaffected chickens were dissected out and analyzed for ash and calcium. The bones were ground, dried, and extracted with ether before analysis. The bones from 3 of the affected birds were normal in appearance and had a normal content of ash 142 BULLETIN No. 279 [July, than that at the poultry farm, because of the prevalence of leg weak- ness, which interfered seriously with the feeding of many of the birds. The severity of this symptom is* illustrated by the three chickens shown in Fig. 8. The average gains and average feed records of the White Wyan- dotte and White Leghorn chickens fed at the poultry farm are given FIG. 8. BIRDS RAISED IN INDIVIDUAL FEEDING PENS IN THE NUTRITION LABORATORY Leg weakness developed in many of the birds in the laboratory lots irre- spective of the amount of salt in the rations.. This condition may have been brought on by the small percentage of cod-liver oil in the rations, or by the confinement of the birds in such small pens. c in Tables 2 and 3. The average daily gains of Lots 1 and 2 receiving 1 and 2 percent of salt in the feed, respectively, were not significantly different for either breed. In Lot 3 the average daily gain of the White Leghorns was slightly higher than the gains for the same breed in Lots 1 and 2. The White Wyandottes in Lot 3, however, gained on the average rather distinctly less per day than the birds of the same breed in Lots 1 and 2. In view of the great variability among the individual gains of Lots 1 and 2, it is probable that the lower average gain in Lot 3 bears no relation to the larger intake of salt. The average daily gain of Lot 4, both with the White Wyandottes and White Leghorns, was slightly less than the average gains of the other groups, probably due to the longer time required by the chickens in this lot to become accustomed to their ration. Lot 5, receiving the same ration as Lot 1, gained on the average at a distinctly better rate, this being true of both the White Wyandotte and White Leghorn chickens that survived thru- out the feeding experiment. and calcium. The bones from 2 of the affected birds were bent, but otherwise normal in appearance, and showed a content of ash and calcium somewhat below that of most of the bones from the unaffected birds.. The bones from 2 of the affected birds were bent, soft at the ends, and possessed a distinctly subnormal percentage of ash and calcium and a subnormal percentage of calcium in the ash. The bones from the unaffected birds were perfectly normal in appearance and contained an average of 58.95 percent ash and 22.35 percent calcium on the fat- and moisture-free basis, and an average of 37.91 percent calcium in the ash. THE TOXICITY OF SALT FOR CHICKENS 143 w 9 *-i x O S5 M O &* C5 i o 2 53 3 3 CO CO 5 TO OOfCOOS 00 CO OOO *OCCO *Tf pro -CN rticooo 2 X5-*00 1588 5 00 p ,_, 8 !! s TflNOO POO-l 111- SpOIN 00 CO CO O O O 00 ill 00 ooo"^ w co Tt* co ir U5 IN CO a> CM si 2 OPOCO OPOCO U5cOi'~' PPOO CO "J iir ^ Tti a TO ? 2 1SI= OOOIN U5COO'~' iir g|2 T-ll-l CO o M o rt j 8 ! r *S3 poooo 3 Sooo TjlSS _ iir -u "500IN s CO 3 00 8 CN *0 8 2 CNOOOK5 ) U5XCO t g So?2!rt a o SIS 3 g g OOOCMIN oo co ^ i" i 1C CO O g o TflNOOCO \ i 9 9 c 3, a 3 i ,s 8OOOO (NCO-1 . : - : T CO 3 CO OOP-* IO IQ O i ' ! CO OPO"5 i C Jl -*3 OOO W (N'OfOrH C5 ^ CO O a POOfi OOt^ opoco rH OOPOO TfcON 2 CO tS COINS rHrt M.H rH i-* -"^ INi-H tN ^ 00 O e> "JOOlN 1 " 1 OOOiN Soocc r-* qj i 1 OPOCO 111- "5 00 O CO T* CO 1-1 111 05 sii= go px (N CO 1-1 TJ1 P lO IN 00 CN CN "5 !r OOO^tJ poo-* -1 - Til CO 1-1 iir OOOIN c e a d | : : 'a i g 3 i ^ >> ^ >i Initial weighl Final weight Gain Average dail Initial weigh Final weight Gain Average dail Initial weigh Final weight Gain Average dail Initial weigh Final weight Gain Average dail Initial weigh Final weight Gain Average dail 144 BULLETIN No. 279 [July, The average daily consumption of feed in the lots kept at the poultry farm was practically the same for Lots 1, 2, 3, and 5, and slightly less for Lot 4. The average amounts of feed required per gram of gain were 6.67 grams for Lot 1, 6.72 grams for Lot 2, 6.80 grams for Lot 3, 7.01 grams for Lot 4, and 6.08 grams for Lot 5. These figures are closely related, of course, to the average daily gains of the lots the greater the average daily gain, the smaller the average amount of feed required per gram of gain. The feed and weight records for the Rhode Island Red chickens were obtained for each individual bird. These records with the group TABLE 3. FEED RECORDS FOR THE WHITE WYANDOTTE AND WHITE LEGHORN LOTS Each lot contained 5 birds of each breed. Lot 1 Lot 2 Lot 3 Lot 4 Lot5 Total feed grams 71 552 grams 69 328 grams 69 765 grams 57 445 grams 61 314 Average daily feed. Average daily gain . Feed per gram gain 85.18 12.76 6.67 82.52 12.27 6.72 83.05 12.21 6.80 75.29 10.74 7.01 85.75 14.10 6.08 averages are given in Table 4. The average daily gains of the five lots were, respectively, 11.64, 12.72, 9.60, 9.87, and 11.74 grams. The average daily feed intakes for the five lots were 79.6. 81.8, 69.8, 76.2, and 76.0 grams. The average economy of gains as expressed in the amount of feed required per gram of gain was approximately the same for Lots 1, 2, and 5, namely, 6.85, 6.50, and 6.66 grams, respect- ively. The average economy of gains in Lots 3 and 4 was distinctly less, namely, 7.99 and 7.80 grams of feed per gram of gain, tho some of the individuals in these lots were as economical in the feed cost of gains as individuals in the other lots. It may be concluded from the results of these feeding experiments that the rate and economy of gains in growing chickens is distinctly retarded by 8 percent of salt in the diet, tho this effect is largely due to a slow adjustment of the appetite of the chickens to such a salty ration. It cannot definitely be concluded whether 4 percent of salt in the diet retards the growth of chickens. Some evidence favor- ing the belief that growth was retarded was obtained with White Wyandottes and Rhode Island Red chickens, tho some of the individ- ual gains obtained on the 4-percent salt ration were as high as or higher than the gains secured on the ration containing only 1 percent of salt. The Rhode Island Red chickens receiving 4 percent of salt showed such great variation among the individual birds that the aver- ages cannot be considered representative of the group. 1926~\ THE TOXICITY OF SALT FOR CHICKENS 145 O) S COOO V M C^ Q t^ GO ic c O O5 rH t rH t^ rHOO5lMlCI>l> t^ OS CO CO rH 1C 00 Tt< Tt* O5 t^ rH CO C 00 Of GO "5 i-( b- >C O >C rH CO CO CO l> O3 * rH rH CO l> I 1 - CO OS CO rH 1C r^ Q Z 1 w o ^ CO O5 O 1C rH t^ * O CO l^ OC iC 1C Gft 1> rH 1C * >C Tt* O *O i I 1C t> r}H CO 00 iO rH IO o M 1 h * CO ^ rH t^ (Nl> l^ (M O OO CO 00 . O5 C 1C rH O5 O5 I> 00 O O rH rH t>. CO CO rt< CO CD O t^ 1C O 1C rH Tf 1C t^ i> r^ ict^ --H as oo m Tti oo o rH CD > CONSUMPTIOl ;hts in grams) " *o -4J c o 1 (M 00 O OO ."cO ^ CO 1C rH (N CO ICO5 * OO (N 'S en *O 49 a 3 2 a> - CO t> -* 1 CD rH rH (N GO 1C iC O t> rH rH CD H 'S W te P=* - S < oT g rH rH +3 O HJ rH CO l^ CO CO CO t^ (MOOOOCOCOI> t-, CO CD C75 rH t^ t- IC COb- OO rH 1C T (N -4-i rS i 1 IM rH rH ICCO t^ oooco'caQic t- i 1 1C "H/ 1 rH t~- O> COOOM O rH rH O O g b 2 3 fe 4. AVERAGE 3 a <; '.a - : 3 a _. be : a * : os fl _, bD H O fc rM O 6 ^^ S ^ j : : ; g o S 'ic^ ' : : >> G S, -S Mfi S-0^3 g Mftiil S)^-3^^^ Sog. ipi^li Qs|l uafeilfl P 00 O5 . OO -N 00 1 00 O> O t** . (O ic . I OO 00 QO5 -COt^- t^coco -t>- iceot- rH m . 1C Q Ifi OO rH ts. t>- t ' O - GO O CO 1C rH CO CN rH 1C & 00 1C O ^oc C35 1C >O Q ^ C55 C^ . t>- Q CO O CN (N CO t OO O rH rH t>- t C ^< 00 >C rH 1C CO 00 1-1 t^ ecco t^ CNOOQTt<-*O5O l> rH GO CO C35 ^f rH U500CC 1> CO CO 00 1C t^. C*rH tN. CO Q l> rH . OO C75 O rH 00 00 CO 1C Oi <* rH CO 1 -t "c3 CO *O -*3 2 -i oo co rf co * QCO 00 1C C COO^t CO rH d 1 O rH CO TABLE ' Lot3 4pe t IC(N O OO O rH t>(M Tj* C< rH in Lot 4 8 rH co a> CO 1C CO I> COQ-^OOOOOOoi t* T^ O5 "*& ^ !> 1C i I CO O rH CO C- : a :'.'.'.'.' o "3 1 : : : : i-d & g ::::: d Z, _* _o 6 a ^ : : : :J o g, |l)dfl : :^ s i ilia-s-s ? Pm3^tftlL P 2-a.s o a -g g^ ^oj^feHQ^^I Q<3 H> 6 a, -J3 M O.H -ra g S I ^Tlfrs-i" C3 , } QJ fe O bfi.2'3 o3^?*i be g. w 2-s.S o'3-g K >>^feHQ|^| Q<3 H^fe 1926] THE TOXICITY OF SALT FOR CHICKENS 147 5 1 o t- -oco * ^H CO CO IM Q O 8i 1-1 l 148 BULLETIN No. 279 {.July, Information concerning the daily salt intake per bird and per kilo- gram of body weight is contained in Table 5 for the White Wyandotte and White Leghorn chickens, and in Table 6 for the Rhode Island Red chickens, the individual data being given in the latter case. The aver- age daily intake of salt for all groups ranges from 0.72 gram to 6.32 grams per kilogram of body weight. The average daily intake of salt TABLE 5. AVERAGE SALT INTAKE PER DAY AND PER KILOGRAM OF BODY WEIGHT FOR THE WHITE WYANDOTTE AND WHITE LEGHORN CHICKENS Each lot contained 5 birds of each breed. Lot 1 Lot 2 Lot3 Lot 4 Lot 5 Average weight grams 1 044 grams 1 064 grams 1 045 grams 955 grams 1 131 Average daily feed 85.18 82 52 83.05 75 29 85.75 Average daily salt intake . Salt intake per kilogram of body weight per day . 0.852 0.816 1.650 1.551 3.322 3.179 6.023 6.306 0.857 0.758 per bird for the lots getting 8 percent in their ration was 6.32 grams for the Rhode Island Red chickens and 6.31 grams for the White Wyan- dottes and White Leghorns. The largest intake of salt per kilogram of body weight per day among the individual Rhode Island Red birds of Lot 4 was 6.87 grams for bird No. 1. The figures just considered refer to the average daily intake of salt for the entire period of feeding. For the individual weeks of the experiment the intake of salt was in general higher in the early weeks. For example, in Lot 4 at the poultry farm, consisting of the White Wyandotte and White Leghorn chickens, the average intake of salt per kilogram of body weight per day for the first 4 weeks of the experiment was, in order, 5.66, 8.37, 8.75, and 6.85 grams. The daily intake of salt per kilogram of body weight for the indi- vidual Rhode Island Red chickens in Lot 4 for all weeks of the experi- ment will be found in Table 7. It is evident that some of the individ- ual birds in this group tolerated 8 to 9 grams of salt per kilogram of body weight per day without developing any pathological symptoms or showing a markedly diminished rate of growth. MINIMUM LETHAL DOSE OF SALT At the conclusion of the feeding experiment the birds were given varying doses of salt, either in the solid form in capsules, or in a 20- percent solution, in order to determine the maximum amount of salt that could be tolerated both in repeated doses and in single doses. In the first tests undertaken in this connection, the birds were given two doses a day at approximately 8:30 a. m. and 4:00 p. m. In these tests the doses were not adjusted to the size of the bird, but in the last test the doses were graduated according to the body weight. 1926'} THE TOXICITY OF SALT FOR CHICKENS 149 The first test, which was made upon Rhode Island Red chickens from the laboratory lots, was concerned with the determination of the maximum dosage of salt that could be tolerated by the birds when given repeatedly at the rate of two doses a day. In the first day of the test two doses of 2, 4, 6, 8, and 10 grams were given groups of 3 or more birds taken from Lots 1 to 5. The salt was given by pipette in a 20-percent solution. During the test the birds had no access to feed, but water was kept before them continually except when the water containers became exhausted at night. All birds getting doses of 6, 8, and 10 grams were dead either on the afternoon of the first day or by morning of the second day. None of the birds getting 2- and 4-gram doses died during the first day. The birds surviving on the morning of the second day were therefore divided into two lots and given, respectively, 4- and 6-gram doses. Of those getting 6-gram doses, 5 survived two doses only, and 2 survived four. Of the birds getting 4-gram doses, 2 survived four doses only, 1 survived six doses, and the other bird, which was the lightest in the lot, tolerated two 4-gram doses daily for about a month with no apparent detriment. The results of the test are summarized in Table 8. The body weights given in this table were the final weights of the birds in the feeding experiment, which terminated exactly one week before this test was started. The symptoms of the birds that died were very similar to those that have been reported in the literature. Intense thirst accompanied the treatment in all cases, and death was preceded by a period of ap- parent stupor in which the bird stood or squatted with closed eyes and hanging head. No pronounced convulsions were noted preceding death. Thru the courtesy of the Laboratory of Animal Pathology and Hygiene, 9 of the birds that succumbed in this test were given post- mortem examination by Dr. E. A. Tunnicliff. The mucous membrane of the digestive tube was in all cases found to be irritated, as evidenced by hyperemia, petechia, 'or ecchymosis of the blood vessels. Conges- tion of the liver was a constant finding. It is evident from this test that 8 grams of salt per day could not be tolerated continuously by a majority of chickens. Doses larger than 8 grams per day were quickly fatal. In continuing the experiments in the individual dosing of birds with salt, the question arose as to whether the consumption of feed had anything to do with the survival period of the birds. The second test was therefore concerned with determining whether a difference in susceptibility to salt poisoning exists between birds in a fasting and a fed condition. Three groups of 6 birds each taken from Lots 1 to 5 at the poultry farm were given, respectively, twice daily, 4-, 5-, and 6-gram doses of salt. In each of the three groups 3 of the birds were I ,1 :r = - ! : h* s I *^ -i = : :J? _- * SriL . - :. ~ - - - - ~-~~. ? : *J^ _^ ^ till - - - 1926] THE TOXICITY OF SALT FOR CHICKENS 151 0> So* CO 1 co' cd co' CO CO , OS*~ ' CO CO *' CD >d 1 1 1 1 3. Concluded 1 o -t-3 g c, (NNCO rH 00 CO CO s 3 Lot 4 8 - 00 t^ 1C (N 00 Tj< 00 CO CO 1 1 5 '53 oi-C S si T3 4_? O d Q) C^ QJ 03 1 O C^ ^- C^ **^ o> ; CO O O 3 1-1 1 I IN I-H d d 1000 i i i- . O"S > I OS COCO rH CO <-H TfJ d d 00 o (H .M t>. jed alt intake. .. dlogram bod. 3 a o fill as a) o 83 8??.s o c> c^ -*^ 152 BULLETIN No. 279 [July, S5 W 3 O 5B H 3 W WOO ci ^ S g 51* 1 1 MO Srt pg I QQ g D i a J4 i ^ THE TOXICITY OF SALT FOR CHICKENS 153 fasted during the test while the 3 remaining birds were given access to feed. The results of this test are summarized in Table 9. The results do not show any marked differences in the survival period, whether the birds were fasting or had access to feed. A slight difference favoring the fasting birds may be noted, but it is question- able whether the difference is significant in view of the different be- havior among birds given the same treatment. All doses proved to be rather quickly fatal, except in the case of one bird receiving 8 grams of salt daily, which survived for 10 days. The salt in this test was fed in the solid form and was administered in capsules. A third test, the results of which are summarized in Table 10, was concerned with the toxicity of 3-, 4-, and 5-gram doses of salt given twice daily. While the results indicate an increased mortality with increasing dosage, it is evident that even the smallest dose could not be tolerated by these birds for any great length of time, altho the birds in Lot 4 consumed an average of over 6 grams of salt daily thruout the feeding experiment. TABLE 8. MINIMUM LETHAL REPEATED DOSE OF SALT FOR RHODE ISLAND RED CHICKENS (Doses given twice daily, approximately 8:30 a. m. and 4:00 p. m.) Bird No. Lot Body weight 1 Size of dose Number of doses taken before death 2.. 5 grams 1 380 grams 4 4 1 5 1 590 42 4 3 4 1 590 4 6 2 4 1 320 4 indefinite 4 4 1 1 500 6 2 3 2 1 450 6 2 1 1 1 360 6 2 2 1 2 1 850 6 2 2 2 1 560 6 3 2 3 3 880 6 3 4 1 4 1 190 6 2 4 5 1 1 430 8 2 4 2 1 300 8 2 4 4 1 400 8 2 4 5 1 300 8 2 5.... 2 1 640 10 2 5 4 1 190 10 2 5 5 1 745 10 2 'These weights were taken 7 days before the first dosage of salt. 2 Received 2 dose i of 2 grams of salt the day before. 'Received 2 doses of 4 grams of salt the day before. 4 This chicken was given 4 grams of salt twice daily, for almost a month, at the end of which time it was in good condition. 154 BULLETIN No. 279 [.July, ^ _ 25 t-H CO -rt 1 hH 1 .1 C3 -^ O ... O o 4 1