4 IC io9 U. S. DEPARTMENT OF AGRIC OFFICE OF EXPERIMENT STATIONS— BULLED A. C. TRUE, Direct EXPERIMEN Metabolism of Matter and Imm in the Human Bod ^n iM^t^ SEPT. 1898- 900 DEP OSITORY I ""WTO. ATWATER, PH. D., AND F. G. BENEDICT, PH. D. WITH THE COOPERATION" OF A. P. BRYANT. M. S., A. W. SMITH, M. S., and J. F. SNELL, Ph. D. WASHIXGTOX: GOYERXMEXT PRINTING OFFICE, 1 9 2 . OFFICE OF EXPERIMENT STATIONS A. C. True, Ph. D— Director. E. W. Allen, Ph. D. — Assistant Director. C. F. Laxgworthy, Ph. D. — Editor and Expert on Foods and Animal Production. NUTRITION INVESTIGATIONS, MIDDLETOWN, CONN. W. O. Atwater, Ph. D. — Special Agent in Charge. C. D. Woods, B. 8. — Special Agent at Orono, Me. F. G. Benedict, Ph. D. — Physiological Chemist. A. P. Bryant, M. S. — Editorial Assistant. R. D. Milner, Ph. B.— Assistant. LETTER OF TRANSMITTAL. U. S. Department of Agriculture. Office of Experiment Stations, Washington, D. C December IS, 1901. Sir: I have the honor to transmit herewith a general report of 13 experiments on the metabolism of matter and energy in the human bodv. by W. O. Atwater. special agent in charge of nutrition investi- gations, and F. G. Benedict, expert in these investigations, with the cooperation of A. P. Bryant. A. W. Smith, and J. F. Snell. Valuable aid was also rendered by Messrs. P. B. Hawk. H. M. Burr. E. Oster- berg. and others. In addition to the details of these 13 experiments on the general subject of the metabolism of matter and energy a con- siderable number of general deductions are drawn from the experi- ments as a whole. These experiments form part of a series which is in progress atMid- dletown. Conn., in cooperation with the Storrs Agricultural Experi- ment Station and Wesleyan University. They were made with the Atwater-Rosa respiration calorimeter. The apparatus and the earlier experiments have been described in previous bulletins of this Office (Nos. 14. 63, and 69). Such experiments as those reported have for their ultimate object the study of the fundamental laws of nutrition. The results obtained are of such a nature as to warrant the conclusion that the respiration calorimeter is a satisfactory instrument of preci- sion. The experiments here described, like those previously reported, yield important data regarding the transformation and the conserva- tion of energy in the body, the demands of the body for nutriment, the effects of muscular work upon that demand, and the nutritive values of different nutrients and different foods. The report is submitted with the recommendation that it be published as Bulletin No. 109 of this Office. Respectfully. A. C. True. Director, Hon. James Wilson. v tary ofAgricultm C X T E X T S Page. Introduction 7 The questions studied 7 General plan of the apparatus and the experiments 9 Test experiments Sampling of the air current for analysis 14 Experiments with men 15 Preparation, sampling, and analysis of foods 16 Determination of nitrates and nitrites in the water of perspiration 18 Record of body weight, temperature, and pulse rate .' 19 Measurement of muscular work 20 Details of the experiments 21 Metabolism experiment No. 11 21 Metabolism experiment ]Jo. 13 32 Metabolism experiment No. 14 40 Metabolism experiment No. 21 51 Metabolism experiment X os. 23 and 24 Metabolism experiment Xo. 25 71 Metabolism experiment Xos. 26 and 28 80 Metabolism experiment Xos. 29 and 31 94 Metabolism experiment Nos. 32 and 34 107 Summary and results of the experiments 121 Scope of the investigations 121 Summary and results of individual experiments 1 25 Food materials supplied and consumed, and the difference in demand by men at rest and at work 128 Elimination of water 131 Elimination of carbon dioxid 136 Elimination of energy 138 Income and outgo of energy 1 44 5 METABOLISM OF MATTER AND ENERGY IN THE HUMAN BODY. INTRODUCTION. The present report gives the details of 13 experiments upon the metabolism of matter and energy in the human body, made at Middle- town. Conn., under the auspices of the U. S. Department of Agricul- ture, in cooperation with the Storrs Experiment Station and Wesleyan University. These experiments, which are in continuation of those reported in earlier bulletins of this series. a were carried on during the years L s, .»s to 1900, with the same respiration calorimeter b and by the same methods. In addition to the experiments reported in the present bulletin. 11 other experiments, which for convenience of reference have been numbered consecutively with these, were made with the same apparatus at Wesleyan University, during the same years, in connection with an independent investigation, and are reported elsewhere. QUESTIONS STUDIED. As has already been explained, the ultimate purpose of experiments with men in the respiration calorimeter is the study of some of the fundamental laws of nutrition, and the whole inquiry is based upon the principle that the chemical and physical changes which take place within the body, and to which the general term "metabolism" is ap- plied, occur in obedience to the laws of the conservation of matter and of energy. No one doubts that the law of the conservation of matter governs its metabolism in the living organism, and it is generally believed that the law of the conservation of energy likewise applies to the metab- olism of energy. Quantitative determinations of the applications of this law are. however, desirable. *U. S. Dept. Agr., Office of Experiment Stations Buls. 44, 63, and 69. b U. S. Dept. Agr., Office of Experiment Stations Buls. 44 and 63. See also Conn. (Storrs) Sta. Rpt. 1897, p. 212, and Physical Review, 9 (1899), pp. 130-163, 214-251. c Memoirs of the National Academy of Sciences, Vol. VIII, Sixth Memoir, 1902. 7 The main subjects proposed for study have been the following: (1) The application of the law of the conservation of energy in the body. An experimental demonstration of the action of this law was the first object sought. The fundamental principle once proven, the apparatus and methods can be utilized for the study of some of the fundamental problems of the science of food and nutrition. (2) The quantities of nutrients and energy metabolized by men under different conditions of rest and muscular and mental exercise. (3) The amounts of nutrients and energy required for internal phys- iological work, as that of respiration, circulation, and digestion. (1) The relation between external muscular work and the nutrients and energy metabolized in its performance. This general problem includes the narrower one of the power of the body as a machine to convert the potential energy of its food and of its previously stored material into muscular energy. Viewed from one standpoint this latter phase of the subject is the same as that to which the expression "the animal body as a prime motor" is frequently applied. From another standpoint it includes the comparison of the animal body with steam engines and other sources of power in respect to the economy with which the energy of fuel is utilized, the fuel in the case of the animal being its food, while in the case of the ordinary machine it is coal, oil. gas. or wood. (5) The transformations of nutrients and energy in mental work. (6) The capacities of the different classes of nutrients to supply the body with material and energy, the proportions in which they may replace one another in building tissue or yielding energy as heat or as muscular work, and their power to protect one another and the mate- rials of the body from consumption. (7) The nutritive values of food materials and the fitting of food to the needs of the consumer. Of the above problems all have received considerable attention except No. 5, which has to do with food and mental work. This has as 3^et been made the especial subject of only one experiment. It is hoped that the study of this problem may also be entered upon in the not too distant future. Besides the problems thus detailed a number of others have received attention. Among these are: (8) The digestion and assimilation of food material-. (9) The quantities of carbon dioxid. water, nitrogen, and other materials excreted by the body as well as the energy given off as heat and as external muscular work under different conditions of work and rest, together with the rates of elimination at different periods of the day and night. In this connection the respiratory quotient has also been considered. (10) The temperature of the body and its variation during different periods of the day and under different conditions of work. rest, and sleep. (11) The need of ventilation in so far as the comfort of the person under experiment is affected by the proportions of carbon dioxid, and water in the air in the respiration chamber. (12) Finally, a large amount of time, thought, and labor has been devoted to the elaboration and testing of the apparatus and methods of experimenting-. Five years were thus used before the first actual experiments with men were made, and more or less attention is being constantly given to the same subject. GENERAL PLAN OF THE APPARATUS AND THE EXPERIMENTS. The description of the apparatus and the methods of manipulation of the experiments have been given with sufficient detail in the former publications already referred to. It is sufficient to say in this connec- tion that the essential features of the apparatus are a chamber large enough to permit a man to stand up and lie down at full length: appli- ances for measuring and analyzing a ventilating current of air; arrange- ments for passing food and drink into the chamber and removing the solid and liquid excreta, all of which were carefully weighed and sampled for analysis; and devices for determining the heat given off from the body of the man in the chamber, and. in work experiments. for determining the heat equivalent of the muscular work done. Measurements were made of income and outgo of both matter and energy in the man's body during the period of the experiment. The chemical analyses included determinations of the total quantities of the nitrogen, carbon, hydrogen, water, and mineral matter of food, drink, and respiratory and excretory products. In obtaining the income and outgo of energy, the potential energy of the food eaten and of the solid and liquid excreta was determined by means of the bomb calorimeter, and the kinetic energy given off by the subject was measured by the respiration calorimeter and accessory apparatus. TEST EXPERIMENTS. Before the respiration calorimeter can be used in such experiment- ing, its accuracy, both as a respiration apparatus and as a calorimeter, should be demonstrated. Two methods have been adopted for testing its accuracy; first, by generating known amounts of heat electrically, and. second, by burning known amounts of pure ethyl alcohol within the respiration chamber and measuring the heat and the chemical products of combustion. The mean of rive electrical tests of the apparatus, made previous to the experiments here reported, showed a variation between the heat actually measured and that generated so small as to be far within the limits of experimental error. The mean of nine experiments, in which known amounts of ethyl alcohol were burned within the chamber of the calorimeter and the carbon dioxid. water, 10 and heat given off were determined, showed variations between the observed and the theoretical amounts no greater than are ordinarily obtained by the usual analytical methods employed in the laboratory. The detailed description of the electrical and alcohol test experiments may be found in earlier bulletins of this series. a Without going further into the details of the experiments it will suffice to say that they were conducted in the same way as the similar test experiments previously reported, by burning within the calorim- eter a known amount of ethyl alcohol in a specially arranged lamp and determining the amounts of carbon dioxid. water, and heat given off. The air current which passed through the chamber and the con- ditions of the experiment were very similar to those when a man was conhned within the apparatus. As stated above, the electrical tests were all made previous to the time of the experiments here reported. Of the other test experi- ments, the details of which have already been published, one. No. 9, was made in the late spring of 1898, at the close of the second of the metabolism experiments here described. During the summer of 1898 no experiments were carried on with the respiration calorimeter. The first test experiment of the fall commenced November 3, 1898. The agreements between the determined and theoretical amounts of carbon dioxid, water, and heat, while not as close as had sometimes been found, were probably Avithin the limits of experimental error, thus indicating that the apparatus was in good working condition. Follow- ing this test came metabolism experiment No. 13, reported in this bul- letin, which was immediately followed by another test experiment, No. 11. About the middle of December, 189S, still another test experiment, No. 12, gave results which indicated that the apparatus was in satis- factory condition, although the measurement of water was not as close as might be desired. This test was followed by metabolism experi- ment No. 14, the details of which are given further on. The calorim- eter was then used for some experiments not reported in this bulletin. At their close test No. 13 was made, which again showed the apparatus to be in satisfactory condition, although the water determined was in excess of the theoretical. Metabolism experiments Nos. 23 and 21 followed the completion of test No. 13, and one week after their com- pletion test No. 14 was made. In this latter test, while the results of the determinations of carbon dioxid and water given off in the appa- ratus agreed very closely with the theoretical, it was found that the heat measured by the calorimeter was 3.2 per cent less than the heat theoretically given off by the combustion of the alcohol. Test experi- ment No. 15, made in December, 1899, showed similar agreements of carbon dioxid and water, and disagreement of heat. It was also found a U. S. Dept. Agr., Office of Experiment Stations Buls. 63, pp. 38-74; 69, pp. 8-17. 11 • by thermal a tests and electrical tests that the measurement of heat by the apparatus was not correct. An electrical test made in the early part of December. 1899. showed a loss of over 3 per cent of the heat put into the apparatus. A thermal test made at this time showed that there was trouble with the thermo-electric elements. b The difficulty apparently lay in a slight short circuit of these at some point, which interfered with the accuracy of the measurements. Just when the short circuiting occurred it is impossible to say. The test experiment preceding metabolism experiments Nos. 23 and 24 showed the apparatus to be in a satisfactory condition. Test experi- ments made shortly after the close of these experiments showed a dis- crepancy in the heat measured as compared with that theoretically given off. There is nothing to indicate whether the trouble occurred before, during, or at the close of this series of metabolism experi- ments or in the short interval immediately following. The agreement of the theoretical energy of the material oxidized in the body and that measured by the calorimeter in metabolism experiments Nos. 23 and 24 would seem to indicate that the trouble did not occur until the close of the series. Had it occurred before or during these experiments the heat as actually determined by the apparatus would doubtless have been about 3 per cent smaller than the amount which would have been measured had the apparatus been in its usual condition. This would make the heat measured by the calorimeter much -mailer in amount than the theoretical heat given oft by the oxidation of material in the body of the subject, the deficiency being larger than has been found in previous experiments. Since it is impossible to determine just when the trouble arose, the details of experiments Nos. 23 and 24 are here given with the values actually obtained, and the supposition is made that the trouble in the apparatus occurred after the close of the experiments. The data are. however, given by which any correction which seems fitting can be applied. After test experiment No. 15. some repairs were made in the apparatus, following which came test experiment No. 16. in which ethyl alcohol was burned in the respiration chamber. In this test the results obtained were very near the theoretical values for carbon dioxid and heat, but nearly 4 per cent more water was found than should have been obtained from the quantity of ethyl alcohol burned. It was thought best, however, not to delay further the metabolism experiments of 1S99-1900. therefore no other test experiments were made until April. 1900, at the close of metabolism experiment No. 34. At this time test experiment No. 17 gave results very similar to those obtained in the previous test experiment, and indicated that the measurements of water in the test experiments were a U. S. Dept. Agr., Office of Experiment Stations Bill. 69, p. 15. b U. S. Dept. Agr., Office of Experiment Stations Bui. 63, p. 17. . 12 over 4 per cent too large. This excess of water may have been due to either or all of several causes. There may have been a small leak whereby air from the room was drawn into the ventilating air current at some point beyond the "freezer'' a for the incoming air; but care- ful examination failed to reveal any noticeable leak inside the chamber, though later experiments implied that there may have been a leak in the "valve box," through which the air passed on its way from the chamber to the meter pump. It is possible that some of the moisture condensed on the outside of the valve box may have found its way into the air current. This would increase the amount of water found by analysis of the outgoing air. but would not materially affect the determination of either carbon dioxid or heat. There may have been evaporation of water which had condensed on the absorbers and walls of the chamber before the test began; however, there could not have been sufficient water present to account for all of the excess. Taking everything into consideration we do not feel warranted in assuming any definite error in the determination of water in metab- olism experiments No-. 25-34. Such error, if it existed, would not affect the nitrogen and carbon balance at all and would hardly affect the heat balance appreciably. From the figures for test experiments Nos. 16 and IT it will be observed that the excess of water found over the theoretical amounted to not far from 1 gram per hour. If we assume in experiments Xos. 25-31 a leak in the air current sufficient to account for about 1 gram of water per hour or 21 grams per day, the values given in column e of Tables 91, 106, 122. and 138 would be too large by a corresponding amount, and the figures in columns f and g of these same tables would be 11 calories too large. This amount would make a maximum error of 0.5 per cent in the heat in the rest experiments (Nos. 25, 26, and 28) and about 0.3 per cent in the work experiments (Xos. 29, 31, 32, and 31). The results of test experiments Xos. 1-9 have already been pub- lished. 1 ' The results of tests Xos. 10-17 are summarized in Table 1, together with the mean results of tests Xos. 1-9. The proportion of absolute ethyl alcohol (by weight) in the commercial alcohol burned in the lamp in the different test experiments was as follows: Test Xo. 10, 91.03 per cent; tests Xos. 11-11. 90.85 per cent: and tests Xos. 15-17, 90.57 per cent. a U. S. Dept. Agr., Office of Experiment Stations Bui. 63. p. 30. b U. S. Dept. Agr., Office of Experiment Stations Buls. 63, p. 50; 69, p. 13. 13 Table 1. — v ch ethyl alcohol was barm Date. ■vember 3 : 11 November 13 12 December 13 to 14, I 13 February 24 to 2£ 14 March 2 15 December 13 to 14. 1 - December 19 to 21, M : to 7, 1 100 9tS 10 to 17 Total • : tests 1 Total of tes- ion. .Alcohol burned In lami Carbon dioxid. Durat quired. Found." Ratio of amount found to amount required. w. Gram*. Gram*. Gram*. I 35 44 666.7 1,159.8 1,163.9 100.3 35 00 751 - . _ " _ 2J 56 619.4 1.075.4 J ' 99.3 . 00 >i5.7 17 - 99.6 ~ - 40 . " - 1. 744. 6 100.3 - 40 128 1.424.9 " - 12 • - " 71 -" 2 99.2 . " " - - ■ 269 11 17 1,320.7 317 _ - _ ■ •• _ '• 31 19, 2 • N Date. Alcohol Duration, burned in lamp. quired. Water. r :r. 1. Ratio of amount found to amount required. vember 3 to i 1898 11 November 15 to 16, 1898 12 December 13 to 14, 1S9S 14 March 29 to 31,1899 15 December 13 to 14. 1899 16 December 19 to 21. 1899 17 April 6 to 7. 1900 Total of tests 10 to 17 2 Total of tests 1 to 9 s 317 2 to 17 h. m. Gram*. Gram*. Per cent. 35 ,, 666.7 -'- " ' 100.1 " 00 -" - 101.0 2j ■ 619.4 24 00 "," " 631.9 643.4 101.8 52 40 1,001.6 1. 159. 7 1 187 a i 2 40 "" 1 : 99.0 _ 12 ■ " " ■ --• " ; _ " ■ 11 " 101. 2 317 . , • 6,063.8 100.6 31 12,264.4 12,379.1 100.9 Heat. -Date. Novem- November 15 to 16. 1898 December 13 to 14. 1898 Februar; _ March 29 to 31,1899 December 13 to 14. 1899 December 19 to 21. 1-99 April 6 to 7, 1900 Total of tests 10 to 17 Total of tests 1 to 9 » N Total of teste J Durat ion. Alcohol burned in lamp. lutred. Found. Ratio of amount found tc amount required. m. Gram*. Calorie*. Calorie*. Per cent. 34 ,, 666.7 i a . 99.5 35 00 751 - 100.5 _- " 619.4 - 3,960.1 99.6 . 00 ~ ' " 52 40 1,001.6 - 96.7 " 40 825 1 ' -• [5, 145. 3] - 12 - " - B 1 i I I - • 100.3 -■ 11 22. 851. 3 22 - " n 20 41,702.8 a m.4 99.9 - 31 64, 554. 1 64.513.3 99.9 * U. B. Dept. Agr.. Office ol Experiment Stations Bui. 69. pp. 13-14. b Omitting the determinations of heat in Nos. 14 and 15. 14 The table shows the duration of the tests, the amount of ethyl alcohol burned in the lamp, and the theoretical amount of carbon dioxid, water, and heat which would have been produced by the com- bustion of the alcohol as compared with the amounts actually found. As previously noted, the agreement between theoretical values and those actually obtained is, as a rule, very close. SAMPLING OF THE AIR CURRENT FOR ANALYSIS. Two methods have been employed for sampling the outgoing current of air for analysis. After this current of air has left the cooling appa- ratus where the major portion of the water has been condensed, small, samples of about 150 liters each during every six-hour period are drawn by the aspirators. The details of this method and the formulas used for calculating and correcting the results are given in a previous publication.* The greatest objections to the use of the aspirators as a method of sampling the air are: (1) The number of corrections for temperature, barometric pressure, and tension of aqueous vapor which have to be made in finding the actual volume of air drawn into the aspirators; (2) the variations in the speed of the motor driving the meter pump and, consequently, in the volume of the ventilating air current, these not being accompanied by corresponding variations in withdrawal of the sample, and (3) the irregularity with which the sample is drawn. By means of an automatic device for regulating the speed at which the water runs out from the aspirators it has been possible of late to make this rate very uniform. At the same time relatively small fluctuations might introduce errors. Thus, if the aspirators emptied more slowly than the normal at a period when the man was giving off more than the average amount of carbon dioxid, as, for instance, when he was weighing the " absorbers" or doing other special work, the measured amount of carbon dioxid. as determined by the aspirators, would be smaller than the amount actually eliminated, and vice versa. The meter-pump method, briefly described in a previous bulletin, 6 affords, theoretically, an almost perfect means of sampling the venti- lating air current. The arrangements for carrying out this method are as follows: The air from each fiftieth stroke of the pump is collected alternately in two tin receptacles, from which it is withdrawn by suc- tion and passed through tubes filled with pumice stone and sulphuric acid, and with soda lime for the absorption of the water and carbon dioxid, respectively. In the earlier experiments in which the meter pump was used for moving the ventilating air current this method of sampling had not been perfected. In test experiments Nos. 9-12 the a U. S. Dept. Agr., Office of Experiment Stations Bui. 63, pp. 34, 35. b U. S. Dept. Agr., Office of Experiment Stations Bui. 63, p. 31. 15 carbon dioxid as determined in the sample drawn by the meter pump was slightly nearer the theoretical than the corresponding values obtained by the aspirators. Variations in the amounts of water as determined in the samples drawn by the meter pump were a little greater than those for carbon dioxid. The comparative results obtained by the two methods in test experiments Nos. 9-12 are shown in the following table: Table 2. — Comparison of determinations of carbon dioxid and water in the ventilating air current by means of the aspirators and of the meter pump. -. Dura- tion. Carbon dioxid. Water. - Theo- retical amount from alcohol burned in lamp. Determined — Theo- retical amount from alcohol burned in lamp. Determined — s By meter pump. By aspirators. By meter pump. By aspirators. h. m, Grams. Grains. P. ct. Grams. P. ct. Grams. Grams. P.ct. Grams. P. Ct. 9 35 55 1, 206. 9 1,199.2 99.4 1,198.9 99.3 809.3 830.6 102.6 807.9 99.8 10 35 44 1, 159. 8 1.163.9 100.3 1,087.0 93.7 772. 5 773.5 100.1 739.7 95.8 11 35 00 1,304.2 1,293.7 99. 2 1.287.9 98.7 869. 8 878.2 101.0 877.8 100.9 12 27 56 1,075.4 1,067.7 99.3 1,083.2 100.7 717. 2 705.7 1 98.4 705.0 98.3 There was on the whole but little difference between the results obtained by the two methods of sampling the ventilating air current, except in the determinations of water in No. 10. Since the meter pump theoretically should give a more accurate sample than the aspirators, and since the experimental evidence shows that the results with the pump were at least as accurate and sometimes more so than those with the aspirators, the determinations of carbon dioxid and water in the sample drawn by the meter pump were employed in the calculation of the results of all metabolism experiments made subse- quent to metabolism experiment No. 11, although check determina- tions by means of the aspirators were continued during several experi- ments. In most of the experiments the discrepancies between the two methods were so insignificant as to be within the limit of experimental error, and show that the determination of carbon dioxid and water may be made with a satisfactory degree of accuracy by either of the methods. EXPERIMENTS WITH MEN. The general plan of the experiments here reported in detail was much the same as that followed in earlier experiments, the results of which have been published in former bulletins of this series. a For the most part the experiments here reported were made in series, each series comprising three or more experiments which fol- lowed one another without intermission and without the subject a TJ. S. Dept. Agr., Office of Experiment Stations Buls. 44, 63, and 69. 16 leaving the calorimeter. As a rule there was a change of diet in respect to one class of ingredients; for example, the replacement of sugar by an isodynamic amount of fat. In all cases a preliminary period, usually of four days' duration, preceded each experiment or series of experiments with the subject in the chamber of the calorimeter. During this preliminary period the subject received the same diet as was to be served in the experiment which would immediately follow. In addition, the excretory products were collected and analyzed, thus making this period both a digestion and a nitrogen metabolism experiment. On the evening of the last day of the preliminary period J:he subject entered the chamber of the respiration calorimeter and usually retired at about 11 o'clock. At about 1 o'clock in the morning the heat measurements were begun in order to get the calorimeter into normal condition for the commence- ment of the second period, or the metabolism experiment proper, at 7 o'clock the following morning. The programme followed during the experiment or series of experi- ments was arranged beforehand. A copy of it was furnished to the subject, and copies were also posted in convenient places for the guid- ance of those outside. PREPARATION, SAMPLING, AND ANALYSIS OF FOODS. The method of preparing, sampling, and analyzing the food mate- rials was the same as that employed in experiments Nos. 9 and 10, already described. a With the exception of milk, the proper quantity of each kind of food, either for each meal or for the day, was placed in glass jars pre- vious to the beginning of the experiment, and materials which might spoil during the course of the experiment, such as meat and bread, were thoroughly sterilized. Butter and sugar, in quantity sufficient for each day, were passed in with the breakfast, and the subject used, as near as he could judge, the proportions called for by the menu for each meal. Sufficient bread for the day was usually passed in at breakfast time in two jars, and as nearly as possible the scheduled amounts used at each meal. Two or more duplicate jars of each food material, as thus prepared, were analyzed, the usual determinations being made accord- ing to the methods already described. The milk was obtained fresh each day; an aliquot portion was taken for the preparation of a com- posite sample for analysis, and the proper amounts for each of the three meals were placed in bottles. In the following table is shown the percentage composition of the different food materials used in the thirteen experiments reported herewith. The values are for, the fresh, edible material as served to the subject. a U. S. Dept. Agr., Office of Experiment Stations Bui. 69, pp. 20-29,88-89. 17 Table 3. — Composition of food materials in metabolism experiments Xos. 11, 13, 14, 21, 23, 24, 25, 26, 28, 29, 31, 32, and 34. Lab- ora- tory No. Food materials ? x P er " \itro- To m ^ Car- bon. 2851 2962 2967 3022 3027 3165 3176 3186 3205 2853 2956 2970 3021 3029 3166 3177 3187 3206 2855 2972 3031 3169 3179 3024 3189 3191 3200 3202 2959 2842 2960 2S40 3004 3168 3193 2963 2968 3032 3164 3192 3204 24S1 3167 3181 3207 2961 2969 Beef, cooked . . do do do ....do ....do do ....do ....do Butter do do do ....do ....do ....do , ....do ....do Milk, skimmed ....do do do do Milk, whole... ....do ....do ....do ....do Milk. whole and skimmed, mixed ... Maize breakfast food. do Wheat breakfast food . Cereal, parched ...l.do do Bread do do do do do do do Ginger snaps do do do Cookies Ginger snaps Sugar 11 13 14 21 23. 24 25 26,28 29,31 32. 34 11 13 14 21 23, 24 25 26,28 29,31 32,34 11 14 23, 24 25 26, 28 21 29 31 32 34 13 11 13,14 11 21-24 25-28 29-34 11 13 14,21 23, 24 25 26, 28 29, 31 32.34 11 25 26-31 32.31 13 14 (») Per ct. 4.40 4.00 4.44 4.46 5.59 5.36 5.41 5. 72 5.13 .15 .19 .19 .21 .17 .25 .26 .20 .20 .52 .61 .58 .68 .67 .51 .66 .65 .66 .58 1.8S 1.78 1.75 1.82 1.87 1.92 L42 1.38 1.27 1.27 1.46 1.42 1.50 1.38 .92 1.03 1.00 .88 .86 1.04 Per et. 17. 21 14. 36 17. 75 16.57 23. 57 19. 62 19.55 20. 89 18.55 64. 76 63.56 62. 55 66.23 69.16 63.37 65.02 65. 11 65. 58 4.01 4.35 4.11 4.76 4.63 7.03 8.20 8.25 7.95 8.20 6.93 44.39 44.81 41.20 41.39 42.20 42. 72 26. 07 29. 49 27. 33 28.05 28.04 27. 76 29.14 42. 73 44.15 44. 32 43.87 44. 65 43.86 42. 10 dro- gen. Per ct. 2.44 2.07 2.64 2.54 3.37 2.76 2.70 2.99 2.66 10.09 10.09 10. 20 10.55 10.52 10.01 10.02 10.44 10.37 . 57 .61 .59 .67 .63 .94 1.26 1.26 1.19 1.21 Water. Pcrct. 66.4 70.7 66.0 66.7 56.6 62.8 62.5 60.3 64.5 12.4 10.1 10.6 8.7 9.5 8.8 9.9 9.2 8.4 90.8 90.2 90.7 89.4 90.0 86. 6 84.5 84.5 85. 4 85.2 1.04 86. 6.42 6.03 6.17 5.94 6.30 3. 82 4.33 4.11 3.98 4.03 3.99 4.30 4.30 6. 45 6.41 6.61 7.20 6.68 6. 7:; 6.48 4.9 5. 7 7.2 6.1 5.6 4.1 12. 7 37.2 41.7 40.4 38.6 39.3 36.5 37.8 4.3 4.9 4.1 3.7 5.2 4.6 Pro- tein (NX 6.25) 11.8 11.1 10.9 11.4 11.7 12.0 8.9 8.6 7.9 7.9 9.1 9. 4 - 5.8 6.4 6.2 5. 5 5. 1 6.5 3.2 8.2 8.3 1.5 .6 1.7 1.4 1.5 5.1 2.8 3.4 2.1 1.6 2.0 6.2 8.6 8.3 7.2 10.8 8.3 D. < 73.4 72. 9 78. 3 80.4 79. 1 hi. 5 45.7 47.9 46.3 47.0 48.9 48. 9 50.8 49.8 79.8 81.6 .8 1.7 2.0 2.1 1.5 1.9 2. 1.2 1.2 1.3 1.3 1.3 1.3 1.3 1.3 2.9 1.7 1.6 2.0 1.9 2.9 .753 4.437 4.467 4.052 4. ('56 4. 136 4.202 2. 575 2. 966 2.710 2.803 2. 930 2.869 4.247 4. 432 4.434 4.434 4.460 4. 407 3. 960 13007— No. 100- • Used in all the experiments. 02 2 The composition of the feces in the experiments here reported is shown in the table which follows. The composition of the urine and the respirator}- products is shown in connection with the tabular details of the individual experiments: Table 4. — Composition of feces in metabolism experiments Nos. 11, 13, 14, 21, 26, 28, 29, 31, 32, and 34- 24, 25, Laboratory No. Experi- ment No. Nitro- gen. Car- bon. Hydro- gen. Water. Pro- tein (Nx 6.25). Fat. Carbo- drates. Ash. Heat of combus- tion per gram, deter- mined. 2850 11 13 14 21 23 24 25 26 28 29 31 32 34 Per ct. 1.59 1.49 1.57 1.62 1.59 1.45 1.38 1.38 1.70 1.44 1.52 1.21 1.38 Per ct. 14.30 15.04 13.49 14.03 14.44 11.64 13.73 11.98 13.61 14.13 15. 19 12.87 13.56 Per ct. 1.98 2.14 1.85 1.94 2.07 1.65 2.02 1.44 1.83 2.03 2.13 1.86 1.93 Per ct. 70.6 69.8 74.2 72.6 69.3 75.7 69.0 72.3 70.6 69.9 67.5 73.2 70.3 Per ct. 9.9 9.3 9.8 10.1 9.9 9.1 8.6 8.7 10.6 9.0 9.5 7.6 8.6 Per ct. 6.4 8.3 6.7 6.3 5.2 4.9 3.8 3.6 5.5 5.1 5.1 4.5 5.8 Per ct. 8.8 7.0 5.8 6.3 8.5 5.4 11.1 8.5 7.3 10.3 11.3 9.6 9.1 Per ct. 4.3 5.6 3.5 4.7 7.1 4.9 7.5 6.9 6.0 5.7 6.6 5.1 6.2 Calories. 1.554 2965 1.689 2974 1.500 3033 1.571 3035 1.610 3036 1.282 3171 1.571 3183 1.340 3185 1. 525 3195 1.574 3197 1.698 3209 1.449 3211 1.475 DETERMINATION OF NITRATES AND NITRITES IN THE WATER OF RESPIRATION. Defren a calls attention to the presence of nitrates, and especially nitrites, in water of exhalation, and suggests the possibility of the nitrogen thus given off being sufficient in amount to take into account in studying the metabolism of nitrogen. It is of great importance in experiments of the nature of these, in which the balance of income and outgo of nitrogen is determined with great care, to take account of any nitrogen which might thus be eliminated. Since in the experiments here reported the products of respiration are passed through a refrigerating system and cooled to a temperature of about —20° C, there is excellent opportunity for a study of this subject. Accordingly, in several of the experiments, and more particularly in work experiment No. 6, already reported. b the water condensed in the 4i freezers" was carefully preserved and tested qualitatively for both nitrates and nitrites. The water con- densed from the incoming air was tested in a similar manner. Similar tests were also made of the water condensed on the surface of the absorbers and collected thence as "drip." ■Technology Quarterly, 9 (1896), p. 238. See also Experiment Station Record, 8, p. 385. b U. S. Dept. Agr., Office of Experiment Stations Buls. 63, pp. 77-85; 69, pp. 47-60. 19 The test for nitrites was made in the usual way. A few cubic cen- timeters of a solution of metaphenylene-diamine in dilute sulphuric acid is placed in a test tube; a few drops of the water to be tested are allowed to fall into the test tube: if nitrites are present, a yellow color is produced. This test is said to show nitrites when present in as dilute a solution as 1 part in 3,000.000, but in no case was any appreciable evidence of nitrites obtained in the water condensed in the freezers. In only one case was there sufficient coloration to warrant the statement that there was even a trace of nitrites present. Singularly enough, while the respired water condensed in the freezers gave no response to the test for nitrites, that condensed on the cooling apparatus in the respiration chamber gave a very marked nitrite reaction. The test for nitrates was made by means of a solution of diphenyl- amine in rather strong sulphuric acid. Two or three cubic centi- meters of this solution is placed in a test tube and about one-half a cubic centimeter of the water to be tested is allowed to flow slowly down the side of the test tube so that the two liquids do not mix. If a deep- blue ring appears at the contact zone of the two liquids, the presence of nitrates is shown. The results of the test for nitrates were very marked, but there appeared to be little difference between the amount in the water of the incoming and outgoing ventilating air current. After making a check experiment with the alcohol lamp burning inside the chamber the amount of nitrates in the outgoing freezer water appeared to be materially increased. This is not sur- prising, as the high temperature of the alcohol flame might naturally be expected to bring about a certain amount of combustion of the nitrogen of the air. The test for nitrates and nitrites in the condensed water of respira- tion indicated that the actual amounts of these were so minute as to be ignored. It seems highly improbable that an}' measurable quantity of nitrogen can be excreted in this manner. RECORDS OF BODY WEIGHT, TEMPERATURE, AND PULSE RATE. The subject weighed himself on a platform scale sensitive to 10 grams with a weight of 75 kilograms and capable of weighing 100 kilograms. The observations of body temperature were made by the use of an ordinary clinical thermometer. They were sublingual in experiments previous to. and axillary in experiments subsequent to. No. 25. In this latter experiment temperatures were measured in both ways. All observations were, of course, made by the subject himself. Under such circumstances it is not improbable that there may have been occasional errors in the thermometer readings; at best, such observa- tions would be less satisfactory than those which would show the actual internal temperature of the body. In later experiments, the 20 results of which are not yet ready for publication, the body temper- ature was measured by a specially devised electrical thermometer inserted in the rectum, by which differences in body temperature of 0.02° could be detected. a The observations of the pulse rate were also made by the subject. How observations made by a person upon himself compare in relia- bility with those made by another person we are unable to sa}^ The reading and recording of weights and measurements of food and excretory products and the other determinations outside the chamber are controlled either by repeating the observations or by having the quantities observed and recorded by two different persons or otherwise. The conditions for the reading and recording of figures for body weight, temperature, and pulse rate by the subject in the respiration chamber do not permit of so careful a system of checks for avoiding errors in the reading and recording of the figures. For this reason, and because of the uncertainties regarding the value of the observations of temperature and pulse rate just named, the figures for body weight, temperature, and pulse rate are hardly as reliable as those for the other determinations. MEASUREMENT OF MUSCULAR WORK. In the rest experiments the subjects moved about within the appa- ratus as little as possible, thus avoiding all unnecessary muscular exer- tion. In the work experiments, however, they were engaged in active muscular work for eight hours each day. The apparatus used for measuring the work in experiment No. 11 consisted of a bicycle belted to a small dynamo, as in experiment No. 6. b The voltage of the elec- tric current generated was measured, and together with calibrations of the apparatus gave sufficient data for computing roughly the amount of work done by the subject. In the work experiments carried on during the winter of 1900 (experiments Nos. 29, 31, 32, and 34) the apparatus was so modified as to give, it is believed, a more accurate measure of the work done. In these experiments the rear wheel of the bicycle was applied directly to a pulley fastened to the shaft of the dynamo, which was mounted upon a rocking base, the necessary tension being secured by means of a coiled spring which kept the pulley of the dvnamo pressed against the tire of the bicycle. This apparatus was calibrated before and after each experiment. Inasmuch, however, as the method of calibration was greatly improved in the experiments made during the winter and spring of 1901, further description of the apparatus and the methods of measuring the work done is not given in the present publication. "Arch. Physiol. [Pfliiger], 88 (1901), No. 9-10, p. 492. b U. S. Dept. Agr., Office of Experiment Stations Bills. 63, p. 76; 69, p. ■*.. 21 DETAILS OF THE EXPERIMENTS. The details of thirteen experiments with three different subjects are recorded in the following- pages. The methods of calculation were in general the same as those described in a previous publication.* In many cases the derivation of the values is indicated algebraically in the column headings of the tables. METABOLISM EXPERIMENT NO. 11. Subject. — E. O.. the laboratory assistant who served as the subject in experiments Nos. 1 and 2, in which the necessary data for estimat- ing the income and outgo of energy were not determined. 1 ' and in experiments Nos. 5-10, in which these values were determined. He was 32 years of age. 5 feet 8 inches in height, and weighed without clothing about 70 kilograms (151 pounds). Occupation during experiment. — Worked S hours a day upon the stationary bicycle just described. During evenings he passed the time in reading, writing, and resting. Duration. — Four days, beginning at 7 a. m. March 22. 1898. The preliminary period of this experiment began with breakfast March 18. The subject entered the calorimeter on the evening of March 21. and remained until 7 a. m. March 26, thus spending live nights and four days in the respiration chamber. Diet. — The diet during the experiment consisted of beef, butter, skim milk, bread, ginger snaps, cereal breakfast foods, sugar, and coffee, and furnished 124 grams of protein and 3. $62 calories of energy per day. This experiment was intended to be much the same as exper- iment No. 9, d with the exception that the diet should furnish a con- siderably larger amount of energy, and a considerable amount of muscular work should be performed each day. The kinds and quanti- ties of food served at each meal were as follows: Table 5. — Diet in metabolism experiment No. 11. Food materials. Breakfast. Dinner. Supper. Total. Beef Grams. 70 -to 1-50 100 60 Grams. 100 40 210 100 Grams. Grams. 170 Butter 40 390 100 50 120 Milk, skimmed Bread 7-50 300 Maize breakfast food 110 Wheat breakfast food 75 Ginger snaps 75 3-5 Sugar 35 40 110 ■U. S. Dept. Agr., Office of Experiment Stations Bui. 69, p. 31. et seq. b U. S. Dept. Agr.. Office of Experiment Stations Bui. 44. C U. S. Dept. Agr.. Office of Experiment Stations Buls. Xos. 63 and 69. *U. S. Dept. Agr.. Office of Experiment Stations Bui. 69. 22 The amount of drink varied from day to day. On the first day the subject was given 600 grams of coffee infusion and 600 grams of water, making a total of 1,200 grams; on the second and third days 600 grams of coffee infusion and 800 grams of water, making a total of 1,400 grams, and on the last day 600 grams of coffee infusion and 1,500 grams of water, making a total of 2,100 grams. The general routine of the experiment is indicated in the following schedule. The statistics of the diary kept by the subject are sum- marized in Table 7: Table 6. — Daily programme — Metabolism experiment No. 11. 7.00 a. m Rise, pass urine, collect drip. 1.50 p. m Begin Avork. weigh absorbers, weigh self 3.50 p. m Stop work, rest 10 minutes, drink stripped and dressed. 200 grams water. 7.45 a.m Breakfast. 4.00 p. m Begin work. 8.20 a. m Begin work. 6.00 p. m Stop work. 10.20 a.m Rest 10 minutes, drink 200 grams 6.30 p. m Supper, change underclothes. water. . weigh self stripped and dressed. 10.30 a. m Begin work. 7.00 p. m Pass urine, collect drip, weigh 12.30 p.m. Stop work. absorbers. 1.00 p. m Pass urine, collect drip, weigh 10.00 p. m Take cover off food aperture, absorbers. retire. 1.15 p. m Dinner. 1.00 a.m Pass urine. Table 7. — Summary of diary — Metabolism experiment No. 11. Time. 1898. Mar. 22, 7.00 a.m.. 12.50 a.m. 7.00p.m.. 10.00p.m. Mar. 23. 7.00a.m.. 12.45 p.m. 7.00p.m.. 10.00p.m. Mar. 24. 7.00 a.m.. 1.00p.m.. 7.00p.m.. 9.20 p.m.. Mar. 25. 7.00 a.m.. 1.00p.m.. 7.00p.m.. 9.15 p.m.. Mar. 26, 7.00 a.m.. Weight of subject. Without clothes. With clothes. minute. ature ' Hygrometer readings. Kilograms. KUogra nts. 70 73. 60 70.27 69.82 69.50 69.48 68.80 68.45 68.26 67. 95 73. 29 73.30 73. 25 72.90 72.60 72.30 72. 23 72.10 Dry bulb. 97 100 Y7.4 <7. 3 22 21.8 21.4 21.8 21.3 21.9 21.5 Wet bulb. °C. 17.2 17.4 17.6 17 19 18.8 18.8 22 19 22 18 21.4 ' 19 21.6 19.4 Amount of work don*:. — As already stated, the subject worked 8 hours each day upon a stationary bicycle so arranged that the work performed was transformed into heat within the apparatus and 23 measured directly with that given off by the subject. A cyclometer registered the number of miles that would have been covered by the same number of revolutions of the wheel, but the amount of work done could hardly have been as large as would be required to propel a bicycle the number of miles thus recorded. It will be observed from the figures in Table 8 that there was considerable difference in the amount of work done on the different days of the study. Table 8. — Record of work done — Metabolism experiment No. 11. Time. (a) Cyclom- Nam- Actual "eter ber of duration reading, miles, of work. (6) Rate. (c) Heat equiva- lent, axbx 0.2378 Mar. 22. 8.20 a. m Total 1898. Seconds. Watts. Calories. 10.20 a. m - 12.40 p. m 3.50 p. ru 6.00 p. m Mar. 23, 8.20 a. m 10.20 a. in 12.30 p. m 3.50 p. m 6.00 p. m Mar. 24, 8.20 a. m 10.20 a. m 12.30 p. m 3.50 p. m 6.00 p. m Mar. 25, 8.20 a. m 10.20 a. m 12.30 p. m 6.00 D. m 154 182 210 238 266 266 294 415 415 447 479 7.200 7.200 7.200 479 514 551 588 644 0) 28 7,200 28 7,200 ,1 43 7,200 J 7,200 | 7,200 J 7.200 7,200 7, 200 7,200 7.200 7,200 7,200 7,200 32 25 91 I 22 26 25 89 85 103 140 1 Cyclometer not working. Detailed data of Income and outgo. — The quantities of nutrients in the food consumed each da}' and the quantities rejected in the feces are shown in Tables 9 and 10. It will be observed by comparison with the results of some of the rest experiments reported in preceding bulletins, that, while the protein is practically the same as in these rest experiments, the energy in the food has been increased more than 800 calories per day in order to supply the necessary energy for the increased muscular activity. It was quite interesting to note that with 24 increased diet and with increased work the total amount of unavailable material excreted in the feces was considerably larger than in experi- ment No. 9. There was not enough difference, however, in the coeffi- cients of availability to show whether muscular work had any marked effect upon the digestion of the food by the subject. Table 9. — Weight, composition, and heat of combustion of foods — Metabolism experiment No. 11. Lab- ora- tory No. Food materials. Weight per day. Water. Pro- tein. Fat. afei & |^|a? 2851 Beef Grama. 170 120 750 300 no 75 75 no Grams. 112.9 14.9 681.0 128. 1 5.4 5.4 3.2 Grams. 46.8 .9 24.4 26. 6 12.9 8.2 4.3 Grams. 7.3 101.0 1.5 4.5 9.0 1.1 4.7 Grams. 1 Grams. " 7.48 | .15 Grams. ■. Grams. 29.26 US Calories. 3°6 2853 Butter 77.71 12.11 4.28 11.46 7 15 930 2S55 2852 Skininied milk .. 37.5 3.90 30.08 137.1 : 4.26 78 91 286 773 2842 Maize breakfast food s0. 7 2. 07 58 7 1 31 48.90 488 2840 Wheat breakfast 30.90 4.52 32.09 4.85 46.31 /.13 304 2841 Ginger snaps Sugar 60. 6 110.0 .69 319 436 Total 1,710 950.9 124. 1 129. 1 484.6 19.86 373. 46 55. 65 3, 862 Table 10. — Weight, composition, and heat of combustion of feces — Metabolism experiment No. 11. Lab- ora- tory No. Weight of feces. Water. Pro- tein. Fat. Car- bohy- drates. Xitro- Car- gen, bon. Hydro- gen. Heat of combus tion. 2850 Total, 4 days Average, 1 day . . . Grams. 563 141 Grams. 398.0 99.5 Grams. 56.4 14.1 Grams. 36.0 9.0 Grams. 49.6 12.4 Grams. 8.96 2.24 Grams. 80.51 20. 13 Grams. 11.15 2. 79 Calories. 875 219 The urine in this experiment w^as collected in 6-hour periods, and the specific gravity and nitrogen content determined. The heat of combustion was determined in a composite sample of the urine for each day, and the carbon and hydrogen in a composite sample for the four days of the experiment. The methods employed for determining the heat of combustion and for computing the carbon and hydrogen in the urine of each day have already been described/ The statistics of the outgo of matter in the urine in this experiment are given in Table 11. a U. S. Dept. Agr., Office of Experiment Stations Bui. 69, pp. 23 and 35. 25 Table 11. — Amount, specific gravity, and nitrogen of urine by six-hour periods — Metab- olism experiment No. 11. Date. Period. *— MESS! Nitrogen. Mar. 22-23 23-24 24-25 25-26 y 7 a. m. to 1 p. m , 1 p. m. to 7 p. m. 7 p. rn. to 1 a. m. 1 a. m. to 7 a. in Gram?. 326. 3-50.6 292. 3 146.8 Total Total by composite. 7 a. m. to 1 p. ni . 1 p. in. to 7 p. m. 7p.m. tola, m, 1 a. in. to 7a.m. Total 206. 5 311. 3 211.0 150.1 878.9 Total by composite 7 a. m. to 1 p. m . 1 p. m. to 7 p. m. 7 p. m. to 1 a. m. 1 a. m. to 7 a. m . 218.3 315. 4 251.0 133.6 Total Total by composite 7am.tolp.rn 1 p. m. to 7 p. m 7 p. m. to 1 a. in 1 a. m. to 7 a. m Total Total by composite Total, 4 days, by periods 7 a. m. to 1 p. m 1 p. m. to 7 p. m 236.0 304.8 273. 4 120. 157. 5 216. 4 Per cent. Grams. 1.0-27 1.027 1.033 1.031 1. 028 1.031 1.036 1.032 1.030 1.030 1.035 1.034 1.031 1. 032 1. 035 1.037 1.032 1.032 1.25 1.34 1.87 2.10 1.76 1.61 2. 26 2.30 1.89 1.62 1.65 1.80 2.31 2.32 2. 05 1.90 4.70 5. 46 1 . 115. 7 17.32 1.115.7 1.53 17.07 3. 63 5.01 4.77 3.45 - 16.37 4.13 5.11 5. 72 3. Id 918. 3 IS. 11 91S.3 2.00 18.37 4.60 5.4^ ! 934. 2 934.2 2. 05 19.15 3. S47. 1 71.47 3. 24 4.10 Table 12. — Daily elimination of carbon, hydrogen, water, and energy in urine — Metab- olism experiment No. 11. Date. Amount rarbon of urine. maroon. Water. Heat of combus- tion. gram. TotaL 1898. Mar. 22-23 Grams. ■ P. ct. j Grams. 1.115.7 ' 12.30 B78.9 11.98 918.3 • ! 12.87 934.2 13.63 P.et. Grams. 3.54 3.45 3.71 3 <*> P. ct. Grams. 1,0.50.1 815. 849.7 Rfil.i Calorie. Calories. 0. 126 141 23-24 24-25 25-26 . 162 142 .129 U8 . 141 132 Total, 4 days 3.S47.1 1-.32 50.78 = 0.38 14.62 92.% 3.576.3 ; 1 ! i (.139) »533 »The heat of combustion of the urine was determined in the composite sample for each day and in the total composite for four days. The heat of combustion of the urine for the experiment as determined in this latter sample amounted to 0.139 calorie per gram, or a total of 535 calories for the urine of the whole experiment. 26 Tables 13-15 give the results of the determinations of carbon dioxid and water in the ventilating air current. The method of calculation of these tables has been described in some detail in connection with the description of results of experiment No. 5, reported in an earlier publication. a The formulas in the column headings in some of the tables indicate the method by which many of the calculations are made. The drip was collected but once a day and the amount has been equally apportioned among the four periods of the day. While this is undoubtedly erroneous, it has, in view of the lack of any data, seemed the most satisfactory plan. Should some other method of subdivision seem desirable, all data are given by means of which com- putations can be readil} T made. The error involved, however, does not affect the final results of the experiments, as the total quantities per day are used in the computations. Table 13. — Comparison of residual amounts of carbon dioxid and water in the chamber at the beginning and end of each period, and the corresponding gain or loss — Metabolism experiment No. 11. End of period. Carbon dioxid. Water. Date. Total amount in chamber. Gain( + ) or loss ( — ) over pre- ceding period. Total amount of vapor remain- ing in chamber. Gain(+) or loss ( — ) over pre- ceding period. Change in weight of ab- sorbers, gain( + ) or loss ( — ). Drip from ab- sorbers. Total amount gained ( + )or lost (-) during the period. 1898. Mar. 22-23 Grams. 29.7 82.3 64.4 30.5 28.7 Grams. Grams. 45.0 54.9 57.1 52.3 47.8 Grams. Grams. Grams. Grams. +52.6 -17.9 -33.9 - 1.8 + 9.9 + 2.2 - 4.8 - 4.5 +194 - 22 - 27 - 28 114.5 114.6 114.6 114.6 318.4 7 p. m 94.8 la.m 82.8 82.1 Total - 1.0 + 2.8 +117 458.3 578.1 1 p.m 23-24 91.3 72.8 26.6 26.8 +62.6 -18.5 -46.2 + .2 56.6 56.8 53.3 53.2 + 8.8 + .2 - 3.5 - .1 +235 + 7 - 2 - 1 152.0 152.0 152.0 152.0 395.8 v 159.2 1 a.m 146. 5 7 a.m 150.9 Total - 1.9 + 5.4 +239 608.0 852.4 1 p.m 24-25 90.9 76.7 25.2 26.3 +64.1 -14.2 -51.5 + 1.1 61.0 63.5 57.3 55.6 + 7.8 + 2.5 - 6.2 - 1.7 + 57 +248 -294 -295 280.1 280.0 280.0 280.0 344.9 7 p.m 530.5 1 a.m - 20.2 7 a.m - 16.7 Total - .5 + 2.4 -284 1,120.1 838. 5 1 p.m 25-26 98.8 82.0 32.3 35.0 +72.5 -16.8 -49.7 + 2.7 62.9 66.7 56.7 56.8 + 7.3 + 3.8 -10.0 + .1 +177 +155 -223 -222 341.7 341.7 341.6 341.6 526.0 7 p.m 500.5 1 a.m 108.6 7 a.m 119.7 Total + 8.7 + 1.2 -113 1, 366. 6 1,254.8 Total, 4 days. +11.8 - 41 3, 553. 3, 523. 8 U. S. Dept. Agr., Office of Experiment Stations Bui. 27 Table 14. — Record of carbon dioxid — Metabolism experiment No. 11. Carbon dioxid. Date. Period. (a) Ventila- tion (number of liters of air). (6) In in- coming air (per liter). (c) In out- going air (per liter). (d) Excess in out- going air (per liter), c-b (e) Total excess in out- going air, dxa. (/) Cor- rection for carbon diox- id in appa- ratus. Cor- rected weight carbon dioxid exhaled by sub- ject, e+f. (*) Total weight of car- bon ex- haled in carbon dioxid, 1898. Mar. 22-23 7 a.m. tolp.m. lp.m.to7p.m. 7 p.m. to la.m. la.m.to7a.m. Liters. 27,737 28,013 28, 740 28,954 Mg. 0.615 .601 .553 .611 Mgs. 14.229 15. 161 9.955 5.474 Mgs. 13. 614 14.560 9.402 4.863 Grams. 377.3 407.9 270.2 140.9 Grams. +52.6 -17.9 -33.9 - 1.8 Grams. 429.9 390.0 236.3 139.1 Grams. 117.3 106.3 64.4 37.9 Total 113, 444 1,196.3 : - 1.0 1,195.3 325.9 23-24 7 a.m. tolp.m. 1p.m. to 7 p.m. 7p.m. tol a.m. la. m. to 7 a.m. 27, 332 27, 437 28,213 28,899 .582 .742 .551 .611 15. 586 20. 249 10.045 5.817 15. 004 19.507 9.494 5.206 410.1 535.2 267.9 150.5 +62.6 -18.5 —46.2 + .2 472.7 516.7 221.7 150.7 128.9 140.9 60.5 41.1 Total 111,881 1, 363. 7 - 1.9 l.Sfil.R 371.4 ' 24-25 7a.m. tolp.m. 1p.m. to 7 p.m. 7p.m. tol a.m. la.m. to 7 a.m. 28, 257 27. 779 28,359 28, 814 .586 .561 .563 .599 17. 465 19.690 9.542 5.312 16.879 476.8 +64.1 19.129 531.4 -14.2 8.979 254.6 ' -51.5 4.713 135.8 + 1.1 540.9 517.2 203.1 136.9 147.5 141.1 55.4 37.3 Total 113, 209 1,398.6 1 ■- .5 1,398.1 381.3 25-26 7 a.m. tolp.m. lp.m.to7p.m. 7 p.m. to la.m. la.m. to 7 a.m. Total Total, 4 days 28, 094 27,648 28,545 28,254 .594 .586 .508 .641 17. 259 22.508 10.060 6.103 16.665 468.2 +72.5 21.922 606.1 -16.8 9. 552 272. 7 -49. 7 5.462 154.3 +2.7 540.7 589.3 223.0 157.0 147.5 160.7 60.8 42.8 112, 541 1.501.3 +8.7 1.510.0 411.8 451,075 5,459.9 4- 5.3 ! 5.465.2 1, 490. 4 28 Table 15. — Record of water — Metabolism experiment Xo. 11, Water. Date. («) tj™-^ Ventila- Penod. tfon (number of liters of air). (b) (c) (d) Excess In in- In out- in out- coming going going air (per air (per air (per liter), liter), liter), c-b. (e) if) Total Con- excess in densed outgo- in ing air, ireez- dxa. ers. „ {9) Correc- tion for water re- maining in cham- ber. (*) Total water exhaled, e+f+g. 1898. Liters, Mg. Mgs. Mg. Grams. Grams. Grams. Gram". Mar. 22-23 7 a.m. to 1p.m. 27. 737 0.888 1.459 0.571 15. 8 '255. 1 318.4 1p.m. to 7 p.m. 28,013 .795 1.315 .520 15. 304. 4 94.8 414.2 7 p.m. to 1a.m. 28,740 .689 1.380 .691 19.9 298.1 82.8 400.8 1a.m. to7a.m. Total 7 a.m. to 1p.m. 28. 954 .681 1.072 .391 11. 3 260. 7 82.1 3.54.1 113,444 62.0 1,118.3 578. 1 1, 758. 4 23-24 27, 332 .^44 1.571 . 727 19. 9 26a 6 395. 8 684. a 1p.m. to 7 p.m.; 27,437 .826 1.345 .519 14. 2 350. 4 159.2 523. 8 7 p.m. to 1a.m. 28,213 .648 1.270 .622 17. 6 341. 6 146.5 505.7 1a.m. to 7 a.m. Total 7 a.m. to 1p.m. 28, 899 .619 1.092 .473 13.7 ' 298.8 150.9 463.4 111, 881 65.4 1,259.4 852.4 2. 177. 2 24-25 28,257 .824 1.640 .816 23. 1 318. 4 344.9 686. 4 1p.m. to 7 p.m. 27.779 .788 1.409 .621 17. 2 357. 8 530.5 905.5 7 p.m. to 1a.m. 28,359 .713 1.429 .716 20. 3 340. 8 - 20.2 340.9 1a.m. to 7 a.m. Total 7 a. m. tolp. m. 28, 814 .670 1.139 .469 13. 5 301. 5 - 16.7 298.3 113, 209 74.1 1,318.5 838.5 2,231.1 " 25-26 28, 094 .875 1.862 .987 27. 7 297. 6 526. 851.3 1p.m. to 7 p.m. 27,648 .782 1.4G3 . 681 18. 8 367. 3 500.5 886.6 7 p.m. to 1a.m. 28,545 .628 1.374 .740 21.3 842.8 108.6 472. 7 1a.m. to 7 a.m. Total Total, 4 days 28, 2.54 .607 1.078 .471 13.3 293.6 119.7 426. 6 112, 541 81.1 1,301.3 1.254.8 2, 637. 2 451. 075 282.6 4,997.5 3. 523. 8 8. 803. 9 29 Table 16 gives the summary of the calorimetrie measurements during this experiment: Table 16. — Summary of calorimetrie measurements — Metabolism experiment Xo. 11. Date. Period. (a) (6) (c) id) Heat » °3£5 ssss? p-r sssl 189S. Mar. 22-23 7 a.m. to , 1p.m. to ; 7 p.m. to la.m. to 23-24 24-25 25-26 Total. 7 a.m. to lp.m. to 7 p.m. to 1 a.m. to 1 p.m. 7 p.m. 1 a.m. 7 a.m. 1 p.m. 7 p.m. 1 a.m. 7 a.m. Total. 7 a.m. to lp.m. to 7 p.m. to la.m. to Total. 7 a.m. to 1 p.m. to 7 p.m. to la.m. to 1 p.m. 7 p.m. 1 a.m. 7 a.m. 1 p.m. 7 p.m. 1 a.m. 7 a.m. nmeter. Correc- tion due to tem- perature of food and dishes. (e) Water vaporized equals total amount exhaled less amount condensed in chamber. Calories. Degree. Calories. Calories. 1.018.9 965.6 534.6 275.4 . 4-0.15 - .25 - .15 + 9.0 +15.0 -9.0 -22.5 14.1 Gram.?. 280. 8 321. 6 313. 2 267. 5 2. 794. 5 -15.0 -36.6 1,183.1 1,032.5 - .20 -12.0 1,364.2 - .40 -24.0 482. 5 - .20 -12.0 272. - .10 - 6.0 13.5 ■ 8.0 297. 3 364.8 £55.7 312. 4 (/) (9) Heat Total used in heat vaporiza- deter- tion of mined. water. a+c+d exO.592. +/• Calories. 166. 2 190.5 185.4 158.4 Calories. 1.171.6 1.157.0 711.0 433.8 roo.5 3. 473. 4 176.0 215. 9 210.6 184.9 1.207.0 1,548.1 705. 1 451.1 3.151.4 -6.0 1. 33C. 2 787.4 3.911.3 1,247.6 1,305.5 428. 9 253. 5 + 3.0 -6.0 -9.0 - 3.0 14.4 2.6 349.3 377. 5 354. 9 313.3 206.8 223. 5 210. 1 185. 5 1.443.0 1.520.4 648.0 442. Total... Total. 4 days . 12. 656. 6 4- 9.0 ; -17.0 1.395.0 825. 9 4,053 4 1.229.0 - .15 - 9.0 -11.9 332.6 196.9 1.405.0 1.436.5 - .10 - 6.0 -17.7 389.9 230.8 1,691.0 510. 4 - .10 1 -6.0 . 354.1 209.6 714.0 299.3 - .05 | - 3.0 . 306.0 181.1 477.4 3.475.2 ... -12.0 - 5.8 1.3*2.6 818.4 4.2-7.4 6.0 5. 290. 9 3. 132 15. 725. 5 Balance of income and outgo of matter and energy. — The original data of income and outgo of matter and energy in this experiment may be found in the preceding tables. Tables 17-20, which follow, summarize the results of the experiment, showing the estimated gain or loss of body material, and comparing the estimated energy of the material oxidized in the body with the' heat actually determined by means of the respiration calorimeter. In Table 17 the figures in the third column, nitrogen in urine, include the nitrogen of perspiration, which amounted to 0.2 gram per day: and the figures for water in res- piratory products in the fifth column of Table 18 include the water of perspiration which was absorbed and removed by the underclothing of the subject, which was changed each night. a U.S. Dept. Agr., Office of Experiment Stations Bui. 69. pp. 24 and 52. 30 Table 17. — Income and outgo of nitrogen and carbon — Metabolism experiment No. 11. Nitrogen. Carbon. Time. (a) In food. (&) In feces. (e) In urine. a id) Gain , (+) ,°^ loss(-) a— (b+c). In food. if) In feces. iff) In urine. (A) In re- spira- tory prod- ucts. o ( 9 Gain (+)or loss (— ), 1898. Grams. Grams. Grams. Grams. Grams. Grams. Grams. Grams. Grams. Mar. 22-23, 7 a. m. to 7 a. m . 19.8 2.2 17.5 +0.1 373.4 20.1 12.3 325.9 + 15.1 23-24, 7 a. m. to 7 a.m. 19.9 2.3 17.1 + .5 373. 5 20.2 12.0 371.4 - 30.1 24-25, 7 a.m. to 7 a.m. 19.8 2.2 18.3 _ .7 373.4 20.1 12.9 381.3 - 40.9 25-26, 7a.m. to 7 a.m. 19.9 2.3 19.4 -1.8 373. 5 20.2 13.6 411.8 - 72.1 Total, 4 days 79.4 9.0 72.3 -1.9 1. 493. 8 80.6 50.8 1.490.4 -128.0 Average,! day 19.8 2.2 18.1 — .5 373. 5 20.2 12.7 372. 6 - 32.0 a Including nitrogen in perspiration, which amounted to 0.2 gram per day. Table 18. — Income and outgo of water and hydrogen — Metabolism experiment Xo. 11. Time. Water. (a) In food. (b) In drink. (c) In feces. id) In urine. (e) In respir- atory prod'- UCtS.a CO Appar- ent loss, a+b-{c ~d+e). 1898. Grams. Grams. Grams. Grams. Grams. Gram*. Mar. 22-23, 7 a. m. to 7 a m 950.9 1,200 99.5 1.0-50.1 1.762.2 - 760.9 23-24. 7 a. m. to 7 a m 950.9 1,400 99.5 815. 2, 190. 4 - 754. 24-25, 7 a. m. to 7 a m 950.9 1,400 99.5 849.7 2/2.56.1 - 8-54.4 2.5-26. 7 a. m. to 7 a 4 days m 950.9 2,100 99.5 861. 5 2,699.2 - 609.3 Total 3. 803. 6 6,100 398.0 3. 576. 3 8. 907. 9 -2, 978. 6 Average . lday... 950.9 1.525 99.5 894.1 2. 227. - 744. 7 Time. Hydrogen. (9) In food. (A) In feces. (*) In urine. (I) ' Appar- ent gain. (m) Loss from water, /-9. {n) Total gain (+) or loss -■./-///. 1898. Grams. Grams. Grams. Grams. Grams. Grams. Mar. 22-23, 7 a. m. to 7 a m 55. 6 2.8 3.5 49.3 84.6 - 35.3 23-24, 7 a. m. to 7 a m 55. 7 2.8 3.5 49.4 S3. 8 - 34.4 24-25. 7 a. m. to 7 a m 55. 6 2.8 3.7 49.1 94.9 - 45. s 2.5-26. 7 a. 4 m. to 7 a davs rn 55. 7 2. 8 3.9 49.0 67.7 - 18.7 Total 222. G 55. 7 11.2 2.8 14.6 3.7 196.8 49.2 331. ^2.7 —134. 2 Avers . 1 dav . . - 33.5 a Includes water from perspiration in clothes 62 fourth day. a total of 104 grams. -3.8 grams first day. 13.2 second day. 25 third day. and 31 Table 19.— Gain or of protein (XX6.25), fat, and water — Metabolism experiment No. 11. Time. Nitrogen gained - or lost (-). (6) Protein gained (+)or - si ax 6.25. (c) Total carbon gained (+)or - Carbon in pro- tein gained — or 6x0.53. Carbon in fat, etc.. gained ( + )or lost (-), c—d. (/) Fat gained (+)or lost (-). 6*0.765. L898, Mar. 22-23, 7 a. m. to 7 a. m 23-24. 7 a. m. to 7 a. m 24-25, 7 a. m. to 7 a. m 25-26,7 a.m. to 7 a.m Total, 4 days Average. 1 day Grams. +0.1 -1.8 Grams. Grams. + 0.6 +15.1 + 3.1 . - 30.1 - 4.4 ; - 40.9 -11.2 Grams. +0.3 -1.6 -2.3 -5.9 Grams. - 14.8 31.7 Grams. - 19.3 - 41.4 - 50.5 - 86.5 ■1.9 .5 -11.9 • 3.0 128.0 32.0 -b. 3 -1.6 121.7 30.4 -159. 1 - 39.7 Time. (9) Total hydrogen h (i) Esfi+ASEtF or lost (— ) Hydrogen Hydrogen in protein in fat gained (+) gained ( + i 6x0.07, or lost ( /xO.12. Hydrogen in water. Water etc.. gained i - i gained (+ i or lost - or lost i — i. A-\9. g-{h-\ 1898. Grams. Grams. Grams. Grams. Grams. Mar. 22-23. 7 a.m. to 7 a.m -35.3 0.0 +2.3 -37.6 - 338.4 23-24. 7 a.m. to 7 a.m -34.4 -.2 -5.0 -29.6 - 266.4 24-25. 7 a.m. to 7 a.m —45.8 —.3 —6.1 -39.4 - 354.6 25-26.7 a.m. to 7 a.m — IS. 7 —.8 —10.4 -7.5 — 67.5 Total. 4 days -134.2 - .9 -19.2 -114.1 -1,026.9 Average. 1 day — 33. 5 — . 2 — 4. S — 28. 5 — 256. 7 Table 20. — Income and outgo of energy — Metabolism experiment No. 11, Time. (a) Heat of | com- bustion I of food (6) Heat M Heat id) Esti- mated beat of com-™ ^"bustion Esti- mated beat of com- bustion of feces. bustion of urine. ot pro- tein Esti- mated energy of ma- terial oxi- dized in the bustion of fat gained ^f r tad£ I i e). iff) Heat deter- mined n ifi 2959 2963 Milk (whole and skimmed) Bread 2960 Maize breakfast food 2961 Cookies Sugar 40.0 l 16.84 158 Total 1,420 927.4 117.1 87.8 270.2 18.74 245.84 : 36.58 1 I 1 2, 596 Table 25, — Weight, composition, and heat of combustion of feces- No. 13. Meta bolisrn exper iment Lab- ora- tory No. Weight of feces. Water. P ™" Fat tern. rdU Carbo- hy- drates. Nitro- Car- gen, bon. Hydro- gen. Heat of combus- tion. 2965 Total, 4 days Average, 1 day . . . Grams. 296 74 Grams. 206.6 51.7 Grams. Grams. 27. 5 24. 6 6.9 6.2 Grams. 20.7 5.2 Grains. Grains. 4. 41 44. 52 1.10 11.13 Grams. 6.33 1.58 Calories. 500 125 It has proved a difficult matter to preserve urine so that there shall be no decomposition of the urea into ammonium carbonate. About 2 cubic centimeters of commercial formalin was added to the composite sample of the urine for each day as a preservative, but owing to una- voidable delays it was, in many instances, impossible to determine the heat of combustion of daily samples until some days after the experi- ment closed. In experiment No. 13 the heat of combustion of the com- posite sample for the four days was found to be 0.081 calorie per gram fresh urine, corresponding to 677 calories for the total urine for the period. Later determinations of the heat of combustion of the daily composites gave results showing that a decomposition of the urine had been going on. This decomposition was not shown by a loss of any appreciable amount of nitrogen, the decomposition products remaining largely in solution. The heat of combustion of the urine was, however, largely reduced. This is easily understood by comparing the heat of combustion of urea with that of ammonium carbonate, the former having a heat of combustion of 2.537 calories per gram as compared with 0.75 for the latter as actually determined in one sample of com- mercial ammonium carbonate. In this experiment, therefore, the heat of combustion of urine for each da}' is computed after the same 35 manner 1 as the carbon, hydrogen, and water-free substance for each day: in other words, it is assumed to vary with the nitrogen and in the same relative proportion. Table 26. — Amount, specific gravity, and nitrogen of urine by six-hour jyeriods — Metab- olism experiment Xo. 13. Date. Period. Amount Specific of urine, gravity. 1898. Nov. 8- 9 Grams. 7 a. m. to 1 p. m 1 p. m. to 7 p. m 7 p. m. to 1 a. rn 1 a. ni. to 7 a. ni 1. 015 1. 012 Nitrogen content. Per cent. Gram*. 5. 98 6.24 9-10 10 Total Total by composite. 7 a. m. to 1 p. m 1 p. m. to 7 p. m 7 p. m. to 1 a. m 1 a. m. to 7 a. m 855. " . .70 1. 65 5.99 175. 3 1. 023 2.89 2,715.7 . 21.10 2,715.7 1.012 .77 20. 91 503.6 550.5 708.2 223.8 1.020 1.017 1.012 1.018 .91 .96 .82 1.39 4.58 5. 29 5.81 3.11 Total 1,986.1 1, 986. 1 18.79 1.013 ; .95 18.87 7 a. m. to 1 p. m _ 524. 2 405. 5 647.7 376.4 1.014 1.022 1.014 1.010 .88 1.27 .91 .79 4.61 10-11 11 5.90 2.97 Total 1,953.8 1,953.8 18.63 Total bv composite 1. 015 .95 18. 56 653.0 374. 3 403.0 275.8 1.013 1. 022 1.020 1.017 . 75 1.33 1.18 1.22 4.90 1 p. m. to 7 p. m 4.98 11-12 12 4.75 3.36 Total 1,706.1 1, 706. 1 17.99 Total b v composite 1.016 1.05 17.91 Total, 4 davs, by periods 76.51 Composite, 4 davs 8, 361. 7 .90 75. 26 Table 27. — Daily elimination of carbon, hydrogen, water, and energy in urine — Metab- olism experiment Xo. 13. Date. Amount r .. Knn xt,-^™-^ -.»•-..,.- Heat of combustion. of urine. Carbon. Hjdrogen. Per gram. Total. N 1898. jv.8-9 9-10 10-11 11-12 Grams. .... 2.715.7 .... 1,986.1 .... 1,953.8 1,706.1 P.ct. Grams. P.ct. Grams. 16.38 4.6 14. .58 4.1 14.45 4.1 13.96 3.9 P. ct. Grams. 2,629.2 1,909.1 1.877.5 1,632.4 Calorie. Calories. 187 166 165 159 Total. 4 day- .... .... 8,361.7 0.71 59.37 0.20 16.7 96.28 8,048.2 0.081 i 677 The amounts of carbon dioxid and water of respiration and perspi- ration during the different periods in this experiment are shown in Tables 28-30. There was but little condensation of water upon the ab>orbers and no "drip." 1 r. s. Dept. Agr., < ufice of Experiment Stations Bui. 60, p. 35. 3(5 Table 28. — Comparison of residual amounts of carbon dioxid and water in the chamber at the beginning and end of each period, and the corresponding gain or loss — Metabolism • jjn riment Xo. 13. Carbon dioxid. Water. Date End of period. Total amount in cham- ber. Gain (+) or loss ( — ) over pre- ceding period. Total amount of vapor re- maining in chamber. Gain ( + ) or loss ( — ) over pre- ceding period. Change in weight of absorbers, gain ( + ) or loss ( — ). Total amount gained ( + ) or lost ( — ) during the period. 1898. Nov. »- 9 7 a . m Grams. 30.8 38.8 41.0 27.6 26.1 Grams. Gran us. 37.6 40.3 42.8 43.6 36.9 Grams. Grams. Grams. + 8.0 + 2.2 -13.4 - 1.5 +2.7 +2.5 + .8 -6.7 +35 +45 + 2 + 2 +37.7 +47 5 + 2.8 7 a. m — 4.7 Total - 4.7 _ .7 +84 +83.3 1 p.m 7 p.m 1 a.m 7 a.m Total 1 p.m 7 p.m 1 a.m 9-16 38.3 37.1 27.6 28.5 +12. 2 - 1.2 - 9.5 + .9 40.1 41.1 39.2 37.2 +3.2 +1.0 -1.9 -2.0 + 7 -39 -10 -10 +10.2 -38.0 -11.9 -12.0 + 2.4 + .3 -52 —51. 7 10-11 36.4 39.8 29.1 29.7 - 7.9 + 3.4 -10.7 + .6 38.6 38.8 38.3 35.6 -1.4 + .2 — .5 -2.7 +48 +12 -23 -24 +49.4 +12.2 -23.5 7 a.m Total -26.7 + 1.2 -1.6 +13 +11.4 -Record of carbon dioxid in ventilating air cur rent No. 13. Table 29. I — Metabolism expert mint Period. (a) Ventila- tion (number of liters of air). Carbon dioxid. In incoming air. (d) {€) (f) (g) „ t , i> r ? c : Corrected (h) Total weight ex- haled, Date. (6) Per liter. (c) Total, ax&. Total In out- excess going inoutgo- air - ing air, d—c. amount amount Remain exhaled 1898. Nov. 8- 9 7 a.m. to 1 p. m. 1 p.m. to 7 p. m. 7 p.m. to 1 a. m. 1 a.m. to 7 a. m. Total Liters. 26,872 26, 795 27, 554 27,448 Kg. 0.641 .623 .579 .618 Grams. 17.2 16.7 16.0 16.9 Grams. Grams. 239.3 I 222.1 239. 1 222. 4 216. 5 200. 5 145.9 | 129.0 Grams. + 8.0 + 2.2 -13.4 - 1.5 Grams. 230.1 224. 6 187.1 127.5 Grams. 62.7 61.3 51.0 :-u. 8 1 OS. 669 66.8 840. 8 774. - 4.7 769.3 209.8 7 a.m. to 1 p.m. 1 p.m. to 7 p.m. 7 p.m. to 1 a.m. 1 a.m. to 7 a.m. Total 9-10 26, 372 26, 072 27,920 27,549 .622 .633 .599 .559 16.4 16.5 16.7 lb A 215. 9 199. 5 216.9 200.4 223. 3 206. 6 145. 8 130. 4 +12.2 - 1.2 - 9.5 + .9 211.7 199. 2 197.1 131.3 57.7 54.3 63. 8 35.8 107,913 65.0 801.9 736.9 + 2.4 739.3 201.6 7 a.m. to 1 p.m. 1 p.m. to 7 p.m. 7 p.m. to 1 a.m. 1 a.m. to 7 a.m. Total 10-11 27, 290 26,362 28, 340 26, 904 . 592 .591 .551 .579 16.2 15.6 15.6 15.6 221.5 205.3 +7.9 218.4 i 202.8 +3.4 224.5 208.9 -10.7 145.7 130.1 + .6 213. 2 206.2 198.2 130.7 58.1 56.2 54.1 35.7 108, 896 63.0 810.1 , 747.1 + 1.2 | 748.3 204.1 Total, 3 days . 325,478 194.8 2.452.8 2,258.0 -1.1 2,256.9 615.5 37 T.'ble 30. —Record of water in ventilating air current — Metabolism experiment No. 18. (a) Water in in- coming air. Water n outgoing air. (g) W (*) t ^ c = t- V (6) (c) (d) (e) (/) - r 053 -2 £ a s s* gg as •_ ~ Date. Period. C1/-A ~-B y- g-3 C pj §2 ©3 •d _•- s — ■ ;J a be o_ 33 92 X B ~% O - a — DO mount densed ers. + l x z 5c 3- o - * 5 . > Ph &H < < Eh -— c E-" L898. Liters. Mgs. Grams. lira ms. Grams. Grams. Gram*. Gram*. Grams. NOV. 8-9 7 a.m. to 1 p.m. 26, 872 1.135 30.5 166.9 50.5 217. 4 186.9 +37.7 224. 6 1 p.m. to 7 p.m. 26, 795 1.108 • 29.7 164.8 44.7 209.5 179.8 +47. 5 227. 3 7 p. m. tol a.m. 27, 554 1.040 28.7 197.1 50.5 247. 6 218.9 + 2.8 221. 7 1 a.m. to 7 a. m. Total 27, 448 .980 26.9 157.6 43.5 201.1 174.2 -4.7 169. 5 108,669 115.8 686.4 189.2 875. 6 759. 8 +83. 3 843. 1 7 a.m. tol p.m. 9-10 26. 372 1.015 26.7 167.8 46.5 214. 3 187.6 +10.2 197.8 1 p.m. to 7 p.m. 26, 072 1.045 27.2 160.7 44.7 205. 4 178. 2 -38.0 140. 2 7 p.m. tol a.m. 27, 920 1.073 30.0 181.9 50.7 232. 6 202.6 -11.9 190.7 1 a.m. to 7 a.m. Total 27,549 .970 26.7 165.7 41.1 206.8 180.1 -12.0 168.1 107,913 110.6 676.1 183.0 859. 1 748.5 -51.7 696. S 7 a.m. to 1 p.m. ' 10-11 27,290 1.013 27.6 163.1 49.0 212. 1 184.5 +49.4 233. 9 1 p.m. to 7 p.m. 26, 362 1.082 28.5 171.7 43.3 215.0 186.5 +12.2 198.7 7 p.m. tol a.m. 28, 340 1. 022 29.0 183.8 51.0 234.8 205.8 -23. 5 182. 3 1 a.m. to 7 a.m. Total Total, 3 day?.. 26, 904 .890 23.9 152.7 38.2 190.9 167.0 -26.7 140 3 108, 896 109 4 671.3 181. 5 852. 8 743.8 +11.4 755. 2 325, 478 335. 4 2, 033. 8 553. 7 2, 587. 5 2, 252. 1 +43.0 2, 295. 1 88 Table 31 summarizes the result of the calorimetric measurements during this experiment. Table 31. — Summary of calorimetric measurements — Metabolism experiment No. IS. Period. (a) Heat measured in terms (b) (c) Change Capacity oftem- correc- perature tion of ofcalo- ealorime- rimeter. tei \d) Correc- tion due to tem- perature oi food and dishes. («) Water vaporized equals total amount exhaled less amount condensed in cham- ber. (/) Beat used in vapori- zation of water. 6X0.592. iff) Total heat deter- mined, - - Nov. 7 a. m. to 1 p. no 1 p. in. t<» 7 j>. m — 7 p. in. t<> 1 a. m — 1 a.m. to 7 a. in Total 7a.m. to 1 p.m 1 p. m. to 7 i>. m 7 i>. m. to 1 a. m 1 a. m. to 7 a. m Total 7 a. m. to 1 ]». in 1 j>. m. to 7 p. m 7 i>. m. to 1 a. m 1 a.m. To 7 a. m Calories. 530.2 161.8 293. 1 Degree. Calories. +0. 02 1 . 2 00 04 2 4 Calories. - 30.3 - 43.4 189.6 S2 219.7 167.5 112.2 108.0 130.1 Calories. 613.3 + .02 +1.2 99. 2 393. 8 1,856.1 - 73.7 759. 1 449.5 2,231.9 9-10 is-"), 8 - .Ul + .6 198.5 .01 - .6 151. 1 - .03 -1.8 - • 04 -'J. 4 - 30.8 190.8 179.2 200.7 178.1 112.5 668.1 106.1 118.8 571.4 106. 1 116. 6 1,742.5 4 - .6 -74.1 748.8 442. s 2.111.8 10-11 485. 3 + . 04 2. 1 196.1 .07 -4.2 10 - -l-fi.O - 29.2 - 14.8 185.9 186.7 164. 3 110.1 568.6 110.5 121.5 567.2 1 ' - .05 -3.0 97.3 U5.2 Total 1,742.0 Total, odays. 5.340.6 1.2 - 74.0 742. 2 139.4 2,108.6 H.8 -221.8 2,2.50.1 Balance of incorm and outgo of matter and energy. — The calculated income and outgo of nitrogen, carbon, hydrogen, and energy are shown in the Table- 32-35. The subject was not as nearly in nitrogen equilibrium as in many of the previous experiments, and the loss of nitrogen on the first day of the experiment was much greater than, on the 2 days following. In connection with these experiments it has become a matter of frequent observation that the elimination of nitrogen in the urine seems to be affected by numerous causes little understood. Thus in this experi- ment the elimination of nitrogen in the urine during the 4 days preliminary to the experiment amounted to 1."..:;. L4.8, 20.9, and lM.4 grams, respectively, while tin 1 nitrogen eliminated in the urine during tie- :; days of the experiment proper amounted to 21.1. 18.8, and 18.6 grams, respectively. It is difficult to understand the exact cause for the large elimination of nitrogen during the 2 days preceding the experiment and the first day in the apparatus. It was doubtless due to some physiological or psychic cause, but well illustrates the large fluctuation which may occur in the excretion of nitrogen without 39 change of diet. It is possible that nervous excitement may affect the excretion of nitrogen and thai the increased elimination of nitrogen here observed may have been due in part to an incident which pro- duced such excitement at about this time. A recent experiment by one of as (F. (i. B.), however, regarding the effect of nervous excite- ment on the elimination of nitrogen gave negative results. In experi- ment No. 13 the elimination of nitrogen on the fourth day, the day on which the experiment would have terminated had there not been an accident. was I s grams, a smaller amount than on any of the preceding day-. Table 32. — Tneonu and outgo of nitrogen Average, l day Hydrogen. (9) In food. (h) In (i) In urine. (0 Appar- ent gain, 9- Lose from water. (n) Total gain i f) or l + m . drams. 1.6 1.6 121.',' -91.5 1.6 4.1 + 5.3 1.6 1.1 + 2.3 L09.8 Lfl 12.8 ■ 92 2 176.1 83.9 1.6 40 Table 34. — Gain or loss of protein (NX 6.25), fat, and water — Metabolism experiment No. 13. Time. («) Nitrogen gained ( + )or lost (-). (ft) Protein gained ( + )or lost(-), ax 6.25. (c) Total carbon gained ( + ) or lost(-). id) Carbon in pro- tein gained (+)or lost (-), 6x0.53. (e) Carbon in fat, etc., gained (+) or lost(-), c-d. (/) Fat gained ,( + )<* lost(-), e -^0.765. 1898. Nov. 8-9, 7 a. m. to 7 a. m 9-10, 7 a. m. to 7 a. m 10-11, 7 a. m. to 7 a. m Total, 3 days -5.6 —35.0 Average, 1 day -1.9 —11.7 Grams. Grams. Grams. Grams. Grams. G mm.-. -3.5 -21.9 + 8.5 -11.6 +20.1 +26.3 -1.1 - 6.9 +18.5 - 3.7 +22.2 +29. -1.0 - 6.2 +16.2 - 3.3 +19.5 +25. 5 +43.2 +14.4 ■18.6 ■ 6.2 +61. +20. +80.8 +26.9 Time. 1898. Nov. 8-9, 7 a. m. to 7 a. m . . 9-10, 7 a. m. to 7 a. m . 10-11, 7 a. m. to 7 a. m Total, 3 days Average, 1 day (9) Total h gained or lost ( - ) (h) Hydrogen ydrogen ™S(+) Grams. -91.5 + 5.3 + 2.3 6x0.07. Grams. -1.5 - .5 - .4 (i) Hydrogen "in fat gained ( + ) or lost (-). /xO.12. Grams. +3.2 +3.5 +3.0 -83.9 -28.0 -2.4 - .8 +9.7 -3.2 Ik) Hydrogen in water, etc., gained (+) or lost ( — ) , g-(h+i). Grains. -93. 2 + 2.3 - .3 -91.2 -30.4 Water gained (+) or lost (-), tx9. Grams. -838.8 + 20.7 - 2.7 820.8 273.6 Table 35. — Income and outgo of energy — Metabolism experiment No. 13. Time. 1898. Nov. 8-9, 7 a. m. to 7 a. m . 9-10, 7 a. m. to 7 a. m 10-11,7 a.m. to 7 a. m Total. 3 days (a) Heat of com- bus- tion of food eaten. Calo- ries. 2,596 2,596 2,596 /, Average, 1 day 2,596 (b) Heat of com- bus- tion of feces. Calo- ries. 125 .125 125 Heat of com- bus- tion of urine. Calo- rie^. 187 166 165 375 125 518 173 (d) Esti- mated heat of com- bus- tion of pro- tein gained (+)or lost («) Esti- mated heat of com- bus- tion of fat gained ( + )or lost Calo- ries. -126 - 40 - 35 Calo- ries. +247 +273 +240 (/) Esti- mated energy of ma- terial oxi- dized in the bodv. a-(b+ c+ d+e). -201 - 67 +760 +253 Calo- ries. 2, 163 2, 072 2,101 (9) (h) Heat deter- mined great- Heat er (+) deter- or less mined. (-) than esti- mated, 9-f. Calo- Calo- ries. ries. 2, 232 + 69 2, 112 + 40 2,108 + 7 w Heat deter- mined great- er (+) or less than esti- mated, h+f. 6,336 2, 112 6, 452 2, 151 +116 Per a nt. +3. 2 +2.0 - .3 +1. METABOLISM EXPERIMENT NO. 14. Subject. — E. O., who served as the subject in experiments Nos. 11 and 13 here reported. His weight without clothing was about 64 kilo- grams (141 pounds). It will be noticed that he had lost about 6 kilo- grams in weight between the last experiment and this one. During the intervening time he had a severe cold. 41 Occupation during experiment. — Reading and writing, with as little muscular and mental activity as possible. The subject was consider- ably more quiet during* this rest experiment than during the previous one. Duration. — Four days, beginning at 7 a. m., December 20, 1898. The preliminary period continued 3 days, beginning with breakfast, December 17, 1898. The subject entered the respiration chamber on the evening of December 19. Diet. — The diet during the experiment was much the same as during the previous experiment, and furnished 94 grams of protein and 2,513 calories of energy per day. The kinds and quantities of food served at each meal and the quantities of drink consumed each day were as follows: Table 36. — Diet in metabolism experiment No. 14. FOOL>. Food materials. Breakfast. Dinner. Supper. Total. Beef. . Grams. 55 20 200 95 50 Grains. 105 25 Grams. Grams. 160 Butter 25 250 110 70 450 Bread 105 310 Maize breakfast food 50 30 21 30 20 20 64 DRINK. Time. Coffee infusion. Grams. 300 Water. Grams. Time. Supper Coffee infusion. Water. Breakfast Grams. 300 Grams. 10.30 a. m 200 10.00 p. m 200 300 Total for day 200 900 600 3.50 p. m The general routine of the experiment is shown in the following schedule. The statistics of the diarv are summarized in Table 38. Table 37. — Daily programme — Metabolism experiment No. 14- 7.00 a. m Rise, pass urine, weigh self , 3.30 p. m Drink 200 grams water. stripped, collect drip, weigh 6.30 p. m Supper. absorbers. J 7.00 p. m Pass urine, collect drip, weigh 7.45 a. m Breakfast. absorbers. 10.30 a. m Drink 200 grams water. 10.00 p. m Drink 200 grams water, weigh self 1.00 p. m Pass urine, collect drip, weigh stripped, take cap off food aper- absorbers. ture, retire. 1.30 p. m Dinner. 1.00 a. m Pass urine. 42 Table 38. — Summary of diary — Metabolism experiment Xo. 14. Date. Weight of subject in under- clothes. Pulse rate per minute. Tempera- ture. Hygrometer readings. Dry bulb. Wet bulb. 1898. Kilograms. °F. °C. «C*. Dec. 20, 7.00 a. m 63. 59 64 99.0 20.8 15.6 10.30 a. m 67 99.2 21.4 17.0 5.30 p. m 65 99.4 21.2 16.4 9.00 p.m 66 99.1 21.3 ' 16.9 10.00 p. m Dec. 21, 7.00 a. m 64.28 63. 59 57 98. 2 21.0 16.2 10.00 a. m 61 98.6 21.4 16.4 2.00 p.m 60 98.2 21.1 16.4 5.30 p.m 62 98.6 21.3 17.1 9.00 p.m 60 21.2 16.6 10.00 p.m Dec. 22, 7.00 a. m 63.96 63. 10 62 98.2 21.4 16.6 3.00 p.m 61 99.4 21. 4 16. 6 10.00 p.m 64. 28 66 99.6 21.4 17.1 Dec. 23. 7.00 a. m 63.23 57 99.5 21.0 16.7 3.00 p.m 62 99.2 21.1 17.1 10.00 p.m 63. 95 60 97.9 21.4 16.9 Dec. 24, 7.00 a. m 62.64 59 97.2 21.4 Detailed data of income and outgo. — The usual determinations of income and outgo of nitrogen, carbon, hydrogen, and energy were made. The quantities of nutrients in the food consumed each day and the quantities rejected in the feces are shown in Tables 39 and 40. Table 39. — WeigM, composition, and heat of combustion of foods — Metabolism experi- ment Xo. 14. Lab- ora- tory No. Food materials. Weight per day. Wuter - Sn. Fat Carbo- hy- drates. Nitro- gen. Car- bon. Hydro- gen. Heat of combus- tion. 2967 2970 2972 2968 2960 Beef Butter Skimmed milk... Bread Maize breakfast food Sugar Ginger snaps Total Grains. 160 70 450 310 50 64 30 a rams. Grams. 105. 6 44. 5 7.4 .8 405.9 17.1 129.3 24.5 2. 9 5. 5 Grams. 6.7 59.9 .5 8.7 4.2 Grams. 22. 5 143. 5 36.5 64.0 23.3 Grams. 7.10 .13 2.75 3.94 .31 Grams. 28.40 43.79 19.58 84.72 22. 41 26.94 13.16 Grams. 4.22 7.14 2. 75 12. 74 3.21 4.15 2.02 Calories. 314 553 198 840 223 253 2969 1.4 2.0 2.5 132 1,134 652. 5 94. 4 82.5 | 289.8 15.12 239.00 36.23 2,513 43 Table 40. — Weight, composition, and heat of combustion of feces — Metabolism experi- ment Xo. 14. Lab- ora- tory No. Weight p Carbo- Xi r Hvdrn Heat of . of Water - te Fat - , h >-" ^en Sn Jen COI ? bus - leces. drates. - ge • tion. 2974 Total. 4 days.. Average, lday Grams. Grams. Grams. Grams. Grams. Gram*. Grams. Grams. Calories. 162.3 21.4 14.7 12.7 3.43 29.52 4.04 328 54.7 40.6 .3.4 3.7 3.2 .86 7. 3S 1.01 S2 The amount and composition of the urine during this experiment are shown in Tables 41 and 42. As usual, the urine was collected in 6-hour periods, from which composite samples were made for the whole day. After the urine for the 6-hour periods had been analyzed for nitro- gen in the usual way. the small remaining- portions were, as has been the custom, discarded, although the daily composite samples and the total composite sample for 4 days were saved until all possibility of their being required for future analysis had passed. Immediately after the urine for the 6-hour periods had been discarded, it was discovered that there were certain errors in the determinations of nitrogen, which rendered the results more or less uncertain. The nitrogen was redetermined in the total composite, and the results of these redeterminations appear in Table 41. These results are used in the calculation of the outgo of nitrogen instead of the sum of those in t~ . t- o ►a li IS c S u (i) Date. (6) * (c) X li C id) o o = 7 - z ~ » 2~.£ < (e) o c o Z '■ = - - < + o CL+3 "•a S3 . 1898. Liters. Mgs. Grams. Grams. drains. Grams. Grams. Grams. Grams Dec. 20-21 7 a.m. tol p.m. 2t; 170 1.023 26.8 195.1 46. 6 241.7 214. 9 - 4.3 210. 6 1 p.m. to 7 p.m. 26, 608 .888 23.6 219.9 41.6 261.5 237. 9 +17.7 255. 6 7 p.m. to 1 a.m. 27,430 .853 23.4 229. 8 45. 1 274. 9 251.5 - 3.2 248.3 1 a.m. to 7 a.m. Total 7 a.m. tol p.m. 27, 525 .870 24.0 222. 2 41.1 263.3 239.3 -17.1 222.2 107.733 97.8 867.0 174.4 1,041.4 943. 6 - 6.9 936.7 21-22 26. 971 .868 23.4 203. 5 40. 7 244. 2 220. 8 - 1.6 219. 2 1 p.m. to 7 p. m. 26, 507 .882 23.4 222. 4 40. 4 262. 8 239.4 +13.9 253.3 7 p.m. to 1 a. m. 27, 130 .886 24.0 220. 1 45.4 265.5 241.5 - 2.3 239. 2 1 a.m. to 7 a.m. Total 26, 989 .894 24.1 215. 7 39.6 255. 3 231.2 - 3.1 228.1 107, 597 94.9 861.7 166. 1 1,027.8 932. 9 + 6.9 939.8 22-23 7 a.m. tol p.m. 26, 093 .884 23.1 203.5 39.0 242. 5 219.4 - 6.1 213.3 1p.m. to 7 p.m. 27,152 .930 25.3 220. 1 42.2 262. 3 237. +26.0 263.0 7 p.m. to 1a.m. 27,485 .952 26.2 241.9 17.1 289.0 262. 8 + 2.0 264. 8 1 a.m. to 7 a.m. Total 7 a.m. tol p.m. 27, 580 .871 24.0 224.4 41.7 266.1 242. 1 -10.4 231.7 108,310 98.6 889.9 170.0 1,059.9 961.3 +11.5 972. 8 23-24 26. 971 .942 25.4 216.2 43.9 260. 1 234.7 -17.3 217.4 1 p.m. to 7 p.m. 27, 059 .927 25. 1 235.6 41.5 277. 1 252. + 9.4 261.4 7 p.m. tol a.m. 27, 818 .874 24.3 235.6 46.5 282.1 257. 8 +10.0 267. S 1 a.m. to 7 a.m. 27, 533 .886 24.4 239.6 41.4 281.0 256.6 - 1.5 255. 1 Total Total, 4 days.. 1 109, 381 99.2 927.0 173.3 1,100.3 1,001.1 + .6 1,001.7 433, 021 390.5 3, 545. 6 683.8 4. 229. 4 3, 838. 9 +12. 1 3,851.0 48 Table 46 summarizes the heat measurements during this experiment. Table 46. — Summary of calorimetric measurements — Metabolism experiment No 14. Date. Period. (a) Heat meas- ured in terms Of G20. (b) Change ot tem- perature of calo- rimeter. (c) Capacity correc- tion of calorim- eter, 6x60. (d) Correc- tion due to tem- perature of food and dishes. (e) Water va- porized equals total amount exhaled less amount con- densed in cham- ber. (/) Heat used in vapori- zation of water, exO.592. (9) Total heat deter- mined. a+c+d +/■ 1898. Dec. 20-21 7 a.m. to 1 p.m... 1 p.m. to 7p.m... 7 p.m. to 1 a.m... 1 a.m. to 7 a.m... Total 7 a.m. to 1 p.m... 1 p.m. to 7 p.m... 7 p.m. to 1 a.m... 1 a.m. to 7 a.m... Total 7 a.m. to 1 p.m... 1 p.m. to 7 p.m... 7 p.m. to 1 a.m... 1 a. m. to 7 a.m... Total 7 a. m.tol p.m... 1 p.m. to 7p.m... 7 p. m.tol a.m... 1 a.m. to 7 a.m... Total Total, 4 days Calories. 546.0 .509.9 444. 3 328. 3 Degree. +0.06 + .05 00 - .05 Calories. +3.6 +3.0 +3.0 Calories. - 20.3 - 43.3 Grams. 220. 6 236.6 256. 3 229. 2 Calorics. 130.6 140.1 151. 7 135. 7 Calories. 659.9 609.7 596.0 467.0 1,828.5 +9.6 - 63.6 942. 7 558. 1 2, 332. 6 21-22 466.5 478.3 364.4 271.5 - .02 - 04 - .05 00 -1.2 -2.4 -3.0 - 24.3 - 42.3 221.2 246. 3 240. 2 228.1 131.0 145.8 142. 2 135. 1 572. 579.4 503.6 406. 6 1,580.7 -6.6 - 66.6 935. 8 554.1 2,061.6 22-23 458. 3 502. 5 408.9 297.0 + .05 - .05 + .05 - .05 +3.0 -3.0 +3.0 -3. - 21.8 - 37.3 221.3 241.0 267.8 234. 7 131.0 142. 7 158. 5 139. 570. 5 604.9 570.4 433. 1, 666. 7 - 59.1 964.8 571.2 2, 178. * 23-24 .503. 7 514.6 403.0 247.7 + .02 00 + .04 00 + 1.2 • +2.4 - 25.4 - 41.4 233.4 253. 4 262. S 252. 1 138.2 1-50.0 155. 6 149.3 617.7 623. 2 561.0 397.0 1,669.0 ^3.6 - 66.8 1,001.7 593. 1 2,198.9 6,744.9 -6. 6 -256. 1 3,845.0 2,276.5 8,771.9 VJ Balana of income of matter and energy. — Tables 47-50 show the income and outgo of nitrogen, carbon, hvclroo-en, and enersrv in this experiment, and I he average for 1 days. Table 47. — Income and outgo of nitrogen and carbon — Metabolism experiment Xo. 14- Nitrogen. Carbon. Time. (a) Kb) (c) Gam {e) ,/ (A) In res- Gain In In Tn In Tn pira- - or food. feces. urine. a— (b food. urine. tory prod- ucts. '-! / + g+h). 1898. Grams. (rnilliS. Grams. Grams. GmtUS. Grams. Grams. Grams. Grams. Dec. 20-21, 7 a.in.toTa. m .. 15.1 1\^ -4.G 230. 7.4 14.1 21-22.7 a.m. to 7 a m.. 15.1 . 8 15.9 -1.6 239. 7.4 12.0 203. 1 + 16.5 22-23, 7 a. m. to 7 a m .. 15. 1 .9 15. 2 -1.0 239. 7.4 11.4 20S. 1 -12.1 23-24,7 a.m. to 7 a m .. 15. 1 15.0 - •" 239. 7.4 11.2 209. 1 -11.3 Total, 4 days ... 60.4 3.4 64. 9 -7. 9 956. 29. 6 48. 7 829. 1 -4\6 Average, 1 day . 15.1 .9 16. 2 —2.0 239. 7.4 12.2 ,07.3 +12.1 Table 48. — Income and outgo of water a ad hydrogen— 'Metabolism experiment Xo. 14. Water. Time. (a) (6) (c) In food. In drink. In feces. (d) In res- piratory In urine. prod- ucts. (/) Appar- ent loss, a+b- l c+d+t ). Dec. 20-21. 7 a.m. to 7 a. m -1-22,7 a.m. to 7 a.m. 22-23. 7 a. m. to 7 a. m Grams. Grams. Grams. 1.500 40.0 652.5 1.500 40.6 652.5 1,500 40.6 652.5 1.500 40.6 Grams. Grams. 1,037.3 936.7 1,416.8 939.8 1,555.3 972.8 1,603.1 1.001.7 Grams. - 137.9 - 244.7 - 416.2 23-24, 7 a.m. to 7 a. m - 492. 9 Total, 4 days 2. 610. 6. 000 102. 4 652.5 1,500 40.6 5,612.5 3,851.0 -1,015.9 1,403.1 902. S — 2&4.0 Hydrogen. Time. (9) In food. (ft) 1 i) (I) Appar- In feces. In urine, ent gain. g— (h+i). (m) Loss from water. y+9. {n) Total gain (+) or loss (-), l+m. 1898. Dec. 20-21, 7 a. m. to 7 a. m Grams. 36. 2 30,. 2 36. 2 Grams. Grams. 1.0 4.1 Grams. 31.1 Grams. + 15.3 - 27.2 - 46.2 Grams. + 16. 4 1.0 3.4 31.8 1.0 3.2 32.0 + 4.6 22-23. 7 a. m. to 7a. m -14.2 23-24, 7 a. m. to 7 a. m 1.0 32.0 - 54.8 -22. S 'total, 4 davs 144.8 36.2 4. 13. 9 126. 9 1.0 3.5 31.7 -112.9 -11.0 - 2s. 2 ! +3.5 13007— No. 109— Mi'- 50 Table 49. — Gain or loss of. protein (NX6.25), fat, and water- No. 14. Metabolism experiment Time. (a) Nitro- gen gained (+) or (&) Protein gained | - | or a 6.25. Grams. —28. 7 -10.0 - 6.3 - 4.4 (c) Total carbon gained (+)or lost -). Carbon in pro- tein gained ( + )or lost (-), 6x0.53. (e) Carbon in fat, etc., gained ( + )or lost 1 - 1, c—d. CO Fat gained ( + )or e-h 0.765. 1898. Dec. 20-21. 7 a. m. to 7 a. m Grams. -4.6 -1.6 -1.0 Grants. - 8.7 +16.5 -12.] -11.3 Grains. -15.2 - 5.3 - 3.3 - 2.3 (rrams. +23.9 -21.8 v +15.4 + 13.6 Crams. +31.2 H 28 5 22-23. 7 a. m. to 7 a. in +20 1 23-24. 7 a. m. to 7 a. m + 17.8 Total. 4 daya -7.9 -2.0 -49. 4 -12.4 -48.6 -12.1 -26. 1 - 6.5 +74.7 +18.6 +97 6 +24.4 Time. (9) (A) (0 Tntal Hydrogen I Hydrogen in protein in fat gained ( + ) gained ( +) hvdrogen SlosU-^^^tC-), orlost( 6x0.07. /+0.12 1898. Grams. Dec. 20-21, 7 a. m. to 7 a. m +46. 4 21-22, 7 a. m. to 7 a. in + 4. 6 22-23, 7 a. m. to 7 a. m -14. 2 23-24, 7 a. in. to 7 a. m - 22. 8 Total. 4 days +14. Average, 1 day + 3. 5 Gram*. -2.0 Grams. + 3.8 + 3.4 + 2.4 + 2.1 -3.4 - .8 + 11.7 + 2.9 (*) Hyrogen in water, etc., gained ( + ) or lost ( — ), 0-ih + i). Grams. +44.6 + 1.9 -16.2 -24.6 (0 Water gained ( + ) or lost (-), fcx9. Grams. +401 + 17 -146 -221 + 51 + 13 Table 50. — Income and outgo of energy — Metabolism experiment No. 14. Time. (a) Heat of com- bus- tion of food eaten. 1898. Dec. 20-21, 7 a. m. to 7 a. m 21-22, 7 a. m. to 7 a. m 22-23, 7 a. m. to 7 a.m 23-24, 7 a. m. to 7 a.m Total. 4 days 10. 052 Average, 1 day 2. 513 Calo- ries. 2. 513 2, 513 2, 513 2, 513 (6) (c) Heat Heat of of com- com- bus- bus- tion of tion of feces. urine. Calo- Calo- ries. ries. 82 164 82 140 82 133 82 131 328 568 82 142 (d) Esti- mated heat of com- bus- tion of pro- tein gained (+) or lost (e) Esti- mated heat of com- bus- tion of fat gained ( + ) or lost (-)• Cain- Calo- ries, ries. -165 +293 - 58 +268 - 36 +189 - 25 I +167 -284 - 71 +917 +229 (!) Esti- mated energy of ma- terial oxi- dized in the body, a- (6 + c+d +e). (9) Heat deter- mined Calo- ru s. 2,139 2,081 2. 145 2, 158 8,523 2.131 Calo- ries. 2,333 2,061 2, 179 2,199 (A) Heat deter- mined great- er (+) or less <-) than esti- mated, P-f- Calo- ries. +194 - 20 + 34 + 41 s, 772 +249 2,193 + 62 (0 Heat deter- mined great- er (+) or less (-) than esti- mated, h+f. Per cent. +9.1 -1.0 + 1.6 -1.9 +2.9 51 METABOLISM EXPERIMENT NO. 21. Subject. — A. W. S.. a physicist, who served as the subject of experi- ment No. 4. made before the respiration apparatus had been perfected as a calorimeter. 8 He was 25 years of ao-e and averaged about 70 kilograms (154 pounds) in weight. Occupation during eosperiment. — Heading, writing, etc.. with as little muscular activity as practicable. Duration. — Three days, beginning at 7 a. m. February 12, 1899. Diet. — The diet consisted of beef, butter, whole milk, bread, parched cereal, and sugar, and furnished 97 grams of protein and 2.26i calories of energy per day. The kinds and quantities of food served at each meal and the quantities of drink at different periods of the day were as follows: Table 51. — Diet in metabolism experiment No. 21. FOOD. Food materials. Breakfast. Dinner. Supper. Total. Beef Butter Milk, whole.... Bread Parched cereal . Sugar Grams. Gi ams. Grams. Grams. 55 105 160 10 13 30 250 175 325 750 55 100 155 310 30 30 45 45 DRINK. Time. Water. Time. Water. Breakfast . . . 10.00 a. m ... Grams. 300 1 200 Supper '. 10.30 p. m Total for dav Grams. 300 200 ; 300 200 Dinner 1. 500 3.30 The general routine of the experiment is indicated in the following schedule. The statistics of the diary kept by the subject are sum- marized in Table 53. below: Table 52. — Daily programme — Metabolism experiment No. 21. 7.00 a. m 7.45 a.m 1.00 p. m Rise, pass urine, weigh self 1.15 p. m stripped and dressed, weigh 6.00 p. m absorbers. 7.00 p. m Breakfast, drink 200 grams 10.30 p.m... water. Pass urine. . . Dinner. . . supper. . . Pass urine. . . Pass urine, weigh self stripped, take cap off food aperture, retire. U. S. Dept. Agr., Office of Experiment Stations Bui. 44, p. 51. 52 Table 53. — Summary of diary — Metabolism experiment No. 21. Weight of subject without clothes. Pulse rate Tempera- ture. Hygrometer readings. Time. per min- ute. Dry bulb. Wet bulb. 1899. Feb. 12, 7.00 a. m Kilograms. 69. 48 70 °F. 97.8 19.80 19.90 19.70 19.75 20. 00 19.80 19.70 19.80 20.00 19. 65 19.80 19.70 20. 30 °a 15.15 12.43 p. m 15. 20 15.40 10.00 p. m 69.80 69.35 64 74 97.1 97.5 15 00 Feb. 13, 7.00 a. m 14 90 12.45 p. m 15. 30 6.50 p. m 15. 10 10.05 p. m 69.84 69.34 61 70 97.1 97.0 15. 40 Feb. 14, 7.00 a. m 15.00 12.45 p. m 15. 50 7.25 p. m 15.80 10.00 p. m 69.43 69. 05 67 72 98.0 97.4 15 80 Feb. 15, 7.00 a. ra 15.70 Detailed data of income and outgo. — The usual statistics of income and outgo of matter and energy are shown in Tables 54-61, which follow. Experiments not reported here, covering 10 consecutive days, pre- ceded experiment No. 21. During this time the bowels were slightly lax. This prevented separation of the feces for the preliminary days from those for experiment No. 21. The total feces for 13 da}^s are therefore recorded, as well as the average per day for this experiment. Table 54. — Weight, composition, and heat of combustion of foods — Metabolism experi- ment Xo. 21. Lab- ora- tory No. Food materials. Weight per day. Water. Pro- tein. Car- Fat. | bohy- drates. Xitro- Car- gen, bon. Hydro- gen. Heat of com- bustion. 3022 Beef Gr - — "■ *^ ~ — u — 1899. Liters. Mg. Grams. Grams. Grams. Grams. Grams. Grams. Grams. Feb. 12-13 7 a.m. to 1p.m.. - 0. 740 19.6 194.5 34.8 229. 3 209. 7 - 0.5 210. 2 1p.m. to 7 p.m.. 20, 0OS .750 20. 1 193.0 36. 8 229. 8 209. 7 - 1.7 211.4 7 p.m. to 1a.m.. 27. 890 . 758 21.2 188.0 43.7 231.7 210. 5 - 1.7 - - - 1 a.m. to 7 a.m.. Total 28, 031 .721 20. 2 179. 9 34.8 214. 7 194.5 - 3.6 190.9 109. 003 81.1 755. 4 150. 1 905. 5 824.4 - 3.1 821.3 13-14 14-15 7 a. m. to lp.m.. 1 p.m. to 7 p.m.. 7 p.m. to la.m.. 1 a.m. to 7a.m.. Total 26, 505 . 739 26, 079 . 740 28, 410 . 775 28.070 .848 19.6 195.9 30.1 232.0 212.4 19.5 198.4 33.s 232.2 212.7 I + .7 22.0 208.9 43.0 252.5 230. ■'> 23.8 192.7 35.9 228.6 201.8 1-6.0 218.0 213. 4 22 x 2 198. 8 7 a. m. to lp.m.. 1 p.m. to 7 p.m.. 7 p.m. tol a.m.. 1 a.m. to 7a.m., 109, 004 84.9 705. 149.4 945. 3 860.4 - 2.0 R58.4 20. 052 . 707 21.3 199. 5 35.6 235. 1 213. v -11.0 224. 8 20. 204 - 21.1 213. 9 34.9 248. 8 227. 7 - .1 227. 27, 251 . 766 20.9 219. 3 40.9 200. 2 239. 3 - 3.3 236. 27, B75 .754 21.0 200.8 35. 3 230. 1 215. 1 -5.9 209. 2 |107,982 Total Total, 3 days.. 326,109 84. 3 833. 5 2.50.3 2.384.8 446.2 2,831.0 2,580.7 3. 4 2. 577. 3 56 Table 61 shows the amount of heat carried away in the water cur- rent and in the water vapor in successive 0-hour periods. Table 61. — Summary of calorirnetric measurements — Metabolism experiment Xo. 21. (a) (6) (c) (<*) W ater va- porized (/) (. 12-18,7 a. m. to? a. in. 18-14,7 a in. to 7 ii.in 14-16, 7 a. in. t<» 7 a. in - Total, :'■ daya .. Average, l day. Total hydrogen 1,-1 IlY.lt drums. - rogen in pro- tein gained b 0.07. drams. i/.-, Hydrogen Hydrogen in fat in water, gained etc., gained • ..i- (+ |OJ lOSi i '. lOSi fx 0.118. g-(h+%) 11.9 11. ii Oram*. -3.0 2.2 Grams. + 6. Water gained /.■ 9. (•rums. + 56 - 48 1.2 -9.0 3.0 31.7 -10.6 Table 65. — Income <<»<] <>uto) energy — Metabolism experiment No. .i. Tim*-. Heat of COm- bustion ,,i food eaten. (b) Heal -i com- bustion of feces. (c) (d) (e) Heatof h ( V>,n beatof com- bustion , co J?" tag. „,, bgg. arlne. ,.„,,„,, gained f tit- ( ft (-)• (_) - Esti- mated of ma- teria] oxi- dized in the body, c+d +e). Heal deter- mined (A) Heat deter- mined great- than esti- mated £/-/• (0 Heat deter- mined great- er (+) or less than mated 1899. Feb.12-13,7 a.m.t<> 7 a. in 13-14,7 a.m. to 7 a.m. 14-15,9 a.m. t<» 7 a.m Calo- ries. 2,264 2,264 2,264 ( 'alo- ri( s. 100 100 100 Goto- Goto- Gafo- /■/'.<. nV.v. ;•/>.-■. 119 -236 132 - 65 -1G8 127 - 32 -297 Calo- ries. 2,281 2,265 2,866 Calo- ries. 2,226 2,263 2,348 Calo. rit s. - 55 - 2 - 18 /'. rt. -2.4 - .1 - .7 Total,:', daya 6, 792 2,264 300 378 - 97 -701 6,912 l(i(i TV, - 39 —234 2.304 6,837 2. 279 - 75 - 25 Average, l day -1.1 METABOLISM EXPERIMENTS NOS. 23 AND 24. These experiments were consecutive and form the hist of the experi- ments of L898-99. Subject. — E. O., as in experiments Nos. 11, 13, and L4, described above. Occupation during experimsiit. — Reading, writing, etc., with as little muscular and mental activity as practicable. Duration. -Three days each. Experiment No. % 2?> began at 7 a. m., March It;, and ended at 7 a. in.. March 19, at which time experiment No. 24 began and continued 3 days. An experiment not here re- volted immediately preceded experiment No. 23, so that the subject actually spent 9 days in the respiration calorimeter. The usual pre- liminary period preceded the whole series of experiments. Diet.- The diet consisted of a basal ration of beef, butter, milk, bread, parched cereal, sugar, and horseradish, furnishing 124 grams of protein and 2,546 calories of energy per day. This basal ration 59 was consumed alone In experiment No. 23, hut was Increased by a supplemental ration of L30 grams of sugar per day in experiment No. 24. The kinds and quantities pf food served at each meal, and of drink at different periods of the day arc given in Tattle 66. Table <><>. — Diet in metabolism experiments Nos. ?S and ?4> FOOD— BASAL KM [ON Pood materials. Breakfast. Dinner. Supper. Grams. Total. Beef Grams, 75 15 350 55 15 40 (i nuns. 75 20 390 100 Grams. i •<< Butter 20 390 155 55 Milk skimmed 1,130 Bread 310 15 40 30 • Used with coffee infusion. chiefly with breakfast and dinner for relish. POOD— SUPPLEMENTAL RATION. Metabolism experiment No. 24 .Sugar, 130 grams. duixk. Time. Breakfast 10.30 a. in Dinner. . . 2.30 p.m.. Coffee infusion. Water. Time. Grams. Grams. 260 Supper .. 200 11.00p.m. 260 Total for day 200 Coffee infusion. Water. Grams. Grams. 260 200 '780 ■ coo • The subject did not always drink the full schedule allowance of coffee and of water. The actual amount of water consumed each day is shown in the second column of Tab The general routine of the experiment is shown by the following schedule. Table 68 summarizes the main statistics of the diary kept by the subject during this series of experiments. Table 67. — Daily programme — Metabolism experiments Nos. 28 and ?4> 7.00 a. m Rise, pass urine. 3 30 p. m Drink 200 grams water. weigh aba ■■ 6.30 p. m Supper 7.45 a. in Breakfast. 7. on p m Pass urine. 10.30 a. m Drink 200 grama water. 11.00 p. m Drink 200 grams water, take cap 1.00 p. m Pass urine. of! food aperture, retire. 1.30 p. m Dinner. 60 Table 68. — Summary of diary — Metabolism experiments Nos. 23 and ,?4. Weight of subject without clothes. Pulse rate per minute. Tempera- ture. Hygrometer readings. Time. Dry bulb. Wet bulb. 1899. Experiment No. 23. Mar. 16, 7.00 a. m Kilograms, 72. 89 56 76 65 58 70 66 56 66 66 60 64 69 58 73 71 56 69 70 60 °F. 97.0 98.9 98.4 97.0 98.0 98.0 96.8 97.6 98.3 96.9 98.5 98.8 97.0 99.0 99.0 96.6 99.'2 99.4 97.8 °C. 20.2 20.0 20.4 20.4 20.0 20.2 20.3 20. 2 20.1 20.2 19.8 20. 2 20.0 20.2 20.4 20. 2 20. 2 20.6 20. 8 °C. 15 3 3.30 p. m 15.4 10.45 a. m 16.0 Mar. 17. 7.00 a. m 72. 67 15. 1 3.30 p. m 15.2 10.50 p. m 15.4 Mar. 18, 7.00 a. m 72. 70 14.6 3.40 p. m 15.0 10.50 p. m 15. 2 Experiment No. 2h. Mar. 19, 7.00 a. m 72.68 14.6 3.30 p. m 14.6 10.50 p. m . . 15.0 Mar. 20, 7.00 a. m 72. 70 14.8 4.00 p. m 10.50 p. m 15.4 is 6 Mar. 21, 7.00 a. rn 72.,: 15.0 3.50 p. m 15.2 10.00 p. m Mar. 22, 7.00 a. m 72. 90 lC.il 16.8 Detailed data of income and outgo. — The kinds and amounts of different food materials and nutrients in the daily diet are shown in Table 69. An experiment not reported here preceded No. 23. No separation of the feces was made between that experiment and No. 23, but it was assumed that the undigested material and the metabolic products would not be essentially different in the two experiments. Charcoal was taken with supper March 18 and with breakfast March 22 in order to mark the feces from the diet during experiment No. 24, which began with breakfast March 19 and ended with supper March 21. Table 70 shows the amount and composition of the feces during the experiments. 61 Table 69. — Weight, composition, and heat of combustion of foods — Metabolism experiments Nos. 28 and 24- Lab- t ° r ^ Food materials No. Weieht per day. Water. Pro- tein. Fat. 3029 3031 3032 3004 3069 Basal ration. Beef Butter Skimmed milk.. Bread Parched cereal.. Sugar Horse-radish Grains. 150 55 1,130 310 45 40 30 Grams. 84.9 5.2 1,025.0 125. 2 2.7 Grams. 52.3 .6 40.7 24. 5 5.1 Grams. 9. 2 47.7 1.1 10.5 .3 Carbo- drates. Nitro- Total ration 1 day. experi- ment No. 23. .. Supplemental ra- tion. Rock candy Total ration 1 | day, experi- ment No. 24. . . 26i>.* 130 ...1,269.8 123.0 68.8 68. S Grams. 54.2 145. 7 36.2 40.0 2. 5 Grams. Grams s. 38 35. 35 .09 38. 03 6. 55 46. 44 3.94 86.95 .82 18.63 16.84 .06 2.70 Car- Hydro- bon. gen. Heat of combus- tion. 27s.o 130. 408.6 19.84 244.94 54. 72 19.84 299.60 Grams, 5.05 5. 79 6.67 12. 34 2. 78 2. 59 .18 Calories. 395 441 462 896 183 158 11 35.40 43.82 2.540 515 3.001 Table 70. — Weight, composition, and heat of combustion of feces — Metabolism expert ments Nos. 23 mid 24. Lab- ora- tory No. Weight of feces. Water. Pro- Carbo- Fat. hy- drates. Nitro- gen. Car- bon. Hvdro- Heat of combus- gen - tion. 3035 3036 Experiment Xo. 23. Total, 6 days Average, 1 day... Experiment Xo. 2U. Total, 3 days Average, 1 day... Grams. 425. 7 70. 9 Grams. 295. 49.2 Grams. 42.1 7.0 Grams. (Trams. 22.1 36.2 3. 7 6. Grams. Grams. Grams. Calories. 6.77 61.47 8.81 6S5 1.13 10.25 1.47 114 270.0 204.4 24.5 13.2 14.6 3.91 31.43 4.46 347 90.0 68. 1 8.2 4.4 4.9 1.30 10. 48 1.49 116 a This period includes the 3 days of the experiment proper and 3 days of a preliminary experiment not reported. here. The urine in these experiments, as in those preceding, was collected at intervals throughout the day. Instead of dividing the 24 hours into four equal periods, however, the da} T was subdivided into two periods of 6 hours, one of 4 and one of 8 hours. The object of this was to avoid waking the subject at 1 o'clock at night, since he experi- enced some difficulty in dropping to sleep readily afterwards. Tables 71 and 72 give the data for the amount and composition of the urine during these experiments. The urine was not collected after the close of the experiments. The urine had begun to decompose before the heats of combustion of the daily samples could be determined, con- sequently the daily elimination of energy has been computed accord- ing to the manner followed under similar circumstances in experi- ments Nos. 13 and 14. 62 Table 71. — Amount, specific gravity, and nitrogen of uriru by 6-hour periods — Metab- olism experwu nts Nos. 93 and S4. Period. Amount of urine. Specific gravity. Nitrogen content Mar. 16-17 E.C]/> rinu ut Nii, 7 a. in. to 1 p. Ill 1 p. in. to 7 i>. m 7 p.m. t<> 11 p. in Grams. 844.8 374.6 581 . 1 1.011 1.010 1.020 1.010 /'/ r a ut. .83 :..7J 1.82 11 p. m. to 7 a. m Total 1^ V|l Total by composite 7 a. in. to 1 p.m 1 p. 111. to 7 ]>. in _ _ ■ l.oia .81 18.62 17-18 402. 9 345.2 697.6 1.017 l.oll l.on 1.010 1.14 . 82 4. 59 7 p.m. toll p.m 11 p.m. to 7 a. m Total Total by compo>ite 7 a.m. to 1 p.m 1 p.m. to 7 p.m 2,280.0 1.012 - 19.61 18-19 312.7 588. 1 432. 1 1.018 1.010 1.017 1.013 1.31 .63 . 96 1 . 05 4.10 4.18 7 p. m. to 11 p. m 5 65 11 p.m. to 7 a. m Total 4.51 l 1 * 47 Total by composite- Expert in' nt No. 7 a. m. to 1 p. m I p.m. to 7 p.m 7 p.m. to 11 p.m II p.m. to 7 a. m Total 1.013 .91 18.17 Mar. 19-20 409.9 770.3 285. 2 76a i 1.010 1.012 1.017 1.010 1.12 .76 1.16 .75 4.58 5.85 3.31 5.70 19 4"i Total by composite 2, 225. 5 1.014 .80 19 81 7 a.m. to 1 p. m '. 1 p.m. to7p.ni 20-2] 344.4 661.8 294. 2 570. 5 1.018 1.013 1.019 1.011 1.19 .-7 1.20 .82 4.10 5 76 7 p.m. to 11 p.m 11 p. m. to 7 a. m Total 3.53 1.68 1^ 07 Total by composite i a. m. to ] p.m I p.m. to 7 p. in 7p.m. toll p. m II p. m. to 7 a. m Total 1,870.9 1.013 .96 17.96 21-22 490.0 651.6 257.1 462. 8 1.012 1.014 1.017 l.on .84 .85 1.12 1.02 4.12 1.72 17 26 Total by composite 1,861.5 1.014 .94 17.56 Sample decomposed before percentage of nitrogen was accurately determined. b Nitrogen in composite taken for computations. 63 Table 72. — Daily elimination of carbon, hydrogen, water, and energy in urine — Metab- olism experiment* Nos. 23 and 24- Date. Amount of urine. Carbon. Hydrogen. Water. Heat of combus- tion. Per gram. Total. 1899. Experiment No. Mar. 16-17 Grams. 2, 299. 1 2,280.0 1,996.2 2,225.5 1,870.9 1,861.5 P. ct. ■ :::::: Grams. 12.11 12. 62 11.90 12. 53 11.64 11.11 P. ct. Gram*. 3. 55 3.70 3.49 3.67 P.ct. Grams. 2. 234. 3 2,212.4 1,932.5 Calorie. Calories. 140 17-18 146 18-19 137 Experiment No. 9U. Mar. 19-20 2,158.5 145 20-21 3.41 3. 26 L,808.6 1.802.0 134 21-22 128 Total. 9 days» 18, 540. 8 0.58 107. 53 0.17 31.52 96.9 17.965.3 1 0.067! 1,242 1,1 »This period includes the 6 days of experiments 23 and 24 and 3 days of a preliminary experiment not reported here. 64 The results of the determination of carbon dioxid and water in the ventilating- air current are given in Tables 73-75: Table 73. — Comparison of residual amounts of carbon dioxid and water in the chamber at the beginning and end of each period, and the corresponding gain or loss — Metabolism expi /•'//" nts Nos. 28 and 24. Date. 1S99. Mar. 16-17 Carbon dioxid. End of period. Gain(+) Total orloss(-) amount in chamber. 17-18 18-19 Mar. 19-20 20-21 Experiment No. 2S. Qram8 7 a. m 24.5 lp.m 44.3 7 p. m 40.9 la. m 29.8 7 a . m 25. 2 Total lp. m 39.9 7 p. m 36. 3 1 a. m 26.7 7 a. m 25.0 Total 1 p. m 45.0 7 p. m 42.1 la. m 27.3 7 a, m 26.4 Total Experimt ni No. %k. lp.m 40.3 7 p. m 34.9 1 a. m 26.6 7 a. m 27. S Total 1 p. m 7 p. m 1 a. m 7 a. m Total over pre- ceding period. Grams. +19.8 -3.4 -11.1 -4.6 Water. Total amount of vapor re- maining in chamber. Grams. 35.2 40.5 43.2 45.6 37.7 + +14.7 -3.6 -9.6 - 1.7 .2 +20.0 -2.9 -14.8 - .9 40.1 41.8 37.4 33.5 Gain ( v Total { I amount orlossV-V am0U1 or loss (— ) eainecK+i over pre- ceding period. Grams. -5.3 - 2.7 + 2.4 -7.9 or lost( — j during the period. 11 Grams. 2.5 + 2.4 + 1.7 - 4.4 -3.9 4.2 39.2 39.7 38.4 34.5 1.3 3.9 + 1.4 +13.9 - 5.4 -8.3 - 1.2 37.9 35.1 39.0 38.9 3. 4 2.8 3.9 .1 + \3 - 2.7 - 2.4 -7.9 + 1. 1.4 3.9 1.8 3.9 - 3. - 1.4 ... - 4.4 - 4.4 45. 6 +17.8 j 40.2 - 1.3 - 1.3 38.6 - 7.0 41.5 + 1.3 - 1.3 29.1 - 9.5 41.1 - .4 - .4 24.1 - 5.0 36. 7 - 4.4 - 4.4 21-22 1 p. m 41.2 + 17.1 39.6 i - 2.9 7 p. m 44.4 - 3.2 44.6 | + 5.0 + 5.0 1 a. m 26.5 -17.9 44.3 - .3 - .3 30.4 + 3.9 46.,, — 2. 5 Total - ('». ?, +10.1 +10.1 1 »The variations in the weights of the absorbers were so small as to be within the limits of error in weighing. They are not taken into account in the calculations. There was no drip. 65 r 4. — Record of carbon dioxid in ventilating air current- Xos. 23 and 24. Metabolism experiments Period. (a) Ventila- tion (number of liters of air). Carbon dioxid (h) In incoming air. (d) In out- going air. (0 Total excess in out- going air, d-c. (/) Correc- tion for amount remain- ing in chamber. (g) Cor- rected amount exhaled by sub- ject, e+f. Total weight Date. (&) Per liter. (c) Total, axb of car- bon ex- haled, <7X T \. 1899. Experiment No. 23. Liter)-. Mg. Grains. Grams. Grams. Grams. Grams. Grams. Mar. 16-17 7 a.m. to 1 p. m ... 24. 857 0.700 17.4 234. 9 217. 5 4-19.8 237. 3 64.7 1 p.m. to 7 p. m... 20.329 .706 18.6 245.9 227. 3 - 3.4 223. 9 61.1 7 p. m to 1 a.m. .. 27, 749 .609 16.9 233. 3 216. 4 -11.1 205. 3 56.0 1 a.m. to 7 a.m... Total 7 a. m. to 1 p. m .. 27, 618 .567 15.7 151.3 135. 6 - 4.6 131.0 35.7 106, 553 68.6 865. 4 796.8 4- .7 797. 5 217. 5 17-18 27, 110 .566 15.4 236. 3 220.9 + 14.7 235. 6 64. 2 1 p.m. to 7 p.m. .. 26, 569 .556 14.8 232. S 218. - 3.6 214.4 58.5 7 p.m. to 1 a.m .. 28, 339 . 582 16.5 225. 7 209. 2 - 9.6 199.6 54.4 1 a. m. to 7 a. m .. Total 7 a. m. to 1 p. m .. 28, 209 16.6 152. 1 135. 5 - 1.7 133.8 36.5 110. 227 63.3 846.9 783. 6 - .2 783.4 213. 6 18-19 26, 108 .566 14.8 248. 3 233. 5 +20. 253. 5 69.1 1 p. m to 7 p. m . . 26, 608 .548 14.6 227.4 212. S - 2.9 209. 9 57.2 7 p.m. to 1 a.m . 27. 694 .577 16.0 235. 9 219. 9 -14.8 205.1 55.9 1 a.m. to 7 a. m. . Total Total. 3 days Experiment No. 2U. 27, 572 .578 15.9 148.7 132.8 - .9 131.9 36.0 107, 982 61.3 860. 3 799.0 + 1.4 800.4 218. 2 324, 762 193. 2 2.572.6 2, 379. 4 + 1.9 2.381.3 649.3 19-20 7 a.m. to 1 p.m .. 27, 538 .580 16.0 241.6 225. 6 +13.9 239. 5 65.3 1 p.m. to 7 p.m .. 27.829 .557 15.5 243. 3 227. 8 - 5.4 222. 4 60.6 7 p. m. to 1 a. m . . 27. 889 . 655 18.2 248.6 230. 4 - 8.3 222. 1 60.6 1 a.m. to 7 a.m Total 7 a. m. to 1 p. m .. 27, 385 .639 17.5 153.7 136. 2 - 1.2 137.4 37.5 110, 641 67.2 887. 2 820. + 1.4 821.4 224. 20-21 26, 295 .564 14.8 256. 4 241. 6 + 17.8 259. 4 70.7 1 p. m. to 7 p. m . . 26, 624 .572 15.2 251.0 235. 8 - 7.0 228. 8 62.4 7 p.m. to 1 a.m .. 27, 671 .601 16.6 237.5 220. 9 - 9.5 211.4 57.6 1 a. m. to 7 a. m .. • Total 7 a.m. to 1 p.m .. 27, 938 .549 15.3 159. 9 144.6 - 5.0 139.6 38.1 108, 528 61.9 904.8 842. 9 -3.7 839.2 228.8 21-22 26, 162 .609 15.9 249.4 233. 5 +17.1 250. 6 68.3 1 p.m. to 7 p.m... 26, 049 .582 15.1 243. 4 228. 3 + 3.2 231. 5 63.1 7 p.m. to 1 a. m .. 27. 71S .575 15.9 277.7 261.8 -17.9 243.9 66.5 1 a.m. to 7 a.m .. Total Total. 3 .lays 27, 370 .571 15.6 165.2 149.6 + 3.9 153.5 41.9 107, 299 62.5 935.7 873. 2 + 6.3 879.5 239. 8 326, 468 191.6 2, 727. 7 2,536.1 + 1.0 2,540.1 692. 6 13007— No. 109—02- 66 Table 75. — Record of water in ventilating air current — Metabolism experiments Nos. /•> and 24. Period. Ventilation | num- ^ her of liters of "a air). Water in incoming air. Water in outgoing air. ( chamber, Total water of n ration and perspi ration, g \ h. Date. (6) u - - (c) X e (d) i = z — — ~ - i -. g BO N = z '- --■- < Amount not condensed • in Ereezera (/) i Total excess nut in outgoing a f-c Experiment 1899. No. 23. Lid rs. Mg. Grams. til-itm*. . m.. 26, 049 .693 18.1 200.1 31.0 231.1 213.0 4-5.0 218.0 7 p.m. tol a. in.. 27, 718 .717 19.9 253. 41.0 294.0 271.1 - .3 1 a.m. to 7 a. m. . Total Total, 3 days. 27, 370 .733 20.1 246. 6 35.9 282. 5 262.4 + 2.5 261.9 107,299 76. 5 B86.6 142.2 L.028.7 952. 2 + 10.1 962. 3 326, 468 239. 2 2, 509. 6 43S. 2, 947. 6 2, 708. 4 + 12.3 2, 720. 7 67 Table 76 shows the amount of heat carried away in the water cur- rent and in the water vapor in successive 6-hour periods of experiments Nos. 23 and 24. Table 76. — Summary of calorimetric measurements — Metabolism experiments Xos. 23 and 24. Date. Period. Heat measured in terms Of Coo. (6) Change of tem- perature of calo- rimeter. (0 Capacity correc- tion of calo- rimeter, 6x60. ! Correc- tion due ' to tem- perature of food and dishes. (c) Water va- porized equals total amount exhaled less amount con- densed in chamber. (/) Heat used in vaporiza- tion of water. ex 0.592. Total heat de- ter- mined, a+c+d +/• 1899. Mar. 16-17 Experiment No. 23. 7 a.m. to 1 p.m > 1 p.m. to 7 p.m 7 p.m. to 1 a.m 1 a.m. to 7 a.m Total 7 a.m. to 1 p.m 1 p.m. to 7 p.m 7 p.m. to 1 a.m 1 a.m.to7 a.m Total 7 a.m. to 1 p.m 1 p.m. to 7 p.m 7 p.m. to 1 a.m 1 a.m. to 7 a. m Total Total, 3 days. Experiment No. tU. 7 a.m. tol p.m 1 p.m. to 7 p.m 7 p.m. tol a.m 1 a.m. to 7 a.m Total 7 a.m. tol p.m 1 p.m.to7 p.m 7 p.m. tol a.m 1 a.m. to 7 a.m Total 7 a.m. tol p.m 1 p.m. to 7 p.m 7 p.m. tol a.m 1 a.m. to7 a.m Total Total, 3 days. Calories. 485. 4 521. 4 452. 2 252. Degree. +0.21 - .07 - .05 + .05 Calories. +12.6 - 4.2 - 3.0 - 3.0 Calories. - 17.5 - 23.9 Grams. 200.3 211.0 257. 1 215. 9 Calories. 118.6 124. 9 152. 2 127. 8 Calories. 599.1 618.2 601.4 382.8 1,711.0 + .14 + 8.4 - 41. 4 1 884. 3 523. 5 2. 201. 5 17-18 477.4 502.4 418.1 302.4 + .01 - .6 — 18.6 - 28.7 211.5 209.8 219.1 190.1 125. 2 124. 2 129.7 112. 5 584.0 598.5 547.8 414.9 1, 700. 3 - .01 - .6 - 47.3 830. 5 491.6 2, 145. 2 18-19 513.8 510.0 448.9 277.7 - 16.0 1Q-> 113.8 117.5 128.3 118. 2 611.6 598. 2 578.4 392. 3 - .02 + .02 - .06 - 1.2 + 1.2 - 3.6 - 28.1 : 198.4 ' 216.8 199.6 1,7.50.4 - .06 - 3.6 - 44.1 | 807.0 477. 8 2, 180. 5 5,161.7 - .09 | + 5.4 -132.8 2,521.8 1.492.9 6, 527. 2 Mar. 19-20 497.6 510. 9 450. 5 278.8 - .01 + .05 | + .6 + 3.0 - 18.5 - 29.9 204.6 207. 4 244.6 222. 8 121. 1 122. S 144.8 131.9 600.8 606.8 595. 3 412 5 - .03 ! - 1.8 1,737.8 + .09 - 5.4 — 48.4 j 879.4 520. 6 2,215.4 20-21 520. 9 515.8 436.8 279.2 - .01 - .04 + .02 - .06 + .6 - 2.4 + 1.2 - 3.6 - 14.3 - 31.8 208.7 219. 4 235. 2 123. 5 129.9 139.2 127. 7 630.7 611.5 577 2 215. 7 403.3 1,752.7 -.07 1 - 4.2 - 46.1 ' 879.0 1 520.3 , 2, 222. 7 21-22 590.0 443.1 569.5 248.9 + ,04 - 2.4 - 14.7 205.6 - 29.9 918 n 121.7 129.1 1621 156.8 699.4 542. 3 731 + .01 : - .6 + .6 273. 8 264.9 406 3 1,851.5 + .04 + 2.4 - 44.6 962.3 569.7 2. 379. 5,342.0 | + 06 + 3.6 -139..1 2,720.7 1,610.6 , 6,817.1 68 Balance of imcoine and outgo of matter and energy. — The income and outgo of nitrogen, carbon, hydrogen, and energy in experiments Nos. 23 and 24 are shown in Tables 77-80. Table 77. — Income and outgo of nitrogen and carbon- cmd 24> Metabolism experiments Xos. 23 Nitrogen. Carbon. Time. (a) In food. (6) In feces. (e) In urine. J<9 Gam ( + )or loss (-),o- (b+c). (e) In food. (/) ■ In feces. iff) In urine. (h) In re- spira- tory prod- ucts. (*) Gain ( + )or loss( — ), e-(f+ 9+h). 1899. Experiment Xo. 23. Grains. Grams. Grams. Grams. Grams. Grams. Grams. Grams. Grams. Mar. 16-17, 7 a. m. to 7 a. m. . 19.8 1.1 18.8 -0.1 244.9 10.2 12.1 217. 5 + 5.1 17-18,7 a.m. to 7 a.m.. 19.9 1.2 19.6 - .9 245.0 10.3 12.6 213.6 + 8.5 18-19,7 a.m. to 7 a.m.. 19.8 1.1 18.5 + .2 244.9 10.2 11.9 218.2 + 4.6 Total, 3 days 59.5 3.4 56.9 - .8 734.8 30.7 36.6 649.3 + 18.2 Average, 1 day 19.8 1.1 19.0 - .3 244.9 10.2 12.2 216.4 + 6.1 Experiment No. 2k. Mar. 19-20, 7 a. m. to 7 a. m 20-21,7 a.m. to 7 a.m 21-22, 7 a. m. to 7 a. m Total, 3 days Average, 1 day 19.8 19.9 19.8 1.3 1.3 1.3 19.4 - .9 18.1 + .5 17.3 +1.2 299. 7 10. 5 299.6 10.5 299.7 10.5 12.5 11.7 11.1 224. 228.8 239.8 + 52.7 + 48.6 + 38.3 59.5 19.8 3.9 1.3 54.8 18.2 + •» + .3 899.0 299.7 31.5 10.5 35.3 692.6 +139.6 11.8 : 230.9 - 46.5 Table 78. — Income and outgo of water and. hydrogen — Metabolism experiments Nos. £3 and 24. Water. Time. (a) In food. (6) In drink. In feces. In urine. (c) In respira- tory prod- ucts. (/) Apparent loss, n~b- {c+cl+e). 1899. Experiment Xo. 23. Gram*. Grams. Grams. Grams. Grams. Grams. Mar. 16-17, 7 a. m. to 7 a. m 1, 269. 8 1,362.8 49.2 2,234.3 884.3 - 535.2 17-18,7 a.m. to 7 a.m 1, 269. 8 1,379.2 49.2 2,212.4 830.5 - 443.1 18-19, 7 a. m. to 7 a. m 1,269.8 1, 378. 49.2 1,932.5 807.0 - 140. 9 Total. 3 davs 3, 809. 4 4, 120. 147.6 6, 379. 2 2, 521. 8 -1,119.2 1, 269. 8 1,373.3 49.2 2, 126. 4 840.6 - 373. 1 Experiment Xo. 2k. Mar. 19-20, 7 a. m. to 7 a. m 1,269.8 1,376.0 68.1 2,158.5 879.4 - 460.2 20-21,7 a.m. to 7 a.m 1,269.8 1,382.4 68.1 1, 808. 6 879.9 - 103.5 21-22,7 a.m. to 7 a. m 1,269.8 1,373.4 68.1 1,802.0 962.3 - 189. 2 Total, 3 days 3, 809. 4 4,131.8 204.3 5, 769. 1 2, 720. 7 - 752. 9 Average, 1 day 1,269.8 1,377.3 68.1 1, 923. 906.9 - 250.9 69 Table 78. — Ircome and outgo of water and hydrogen — Metabolism experiments Xos. 23 and 24 — Continued. Hydrogen. Time. iff) In food. (h) In feces. (*) In urine. (*) Apparent gain, g— (h + i). (m) Loss from water, /-9. (n) Total gaiii(-) or loss (-).l+m. 1899. Experiment No. 23. Grams. 'Grams. Grams. Grams. Grams. Grams. Mar. 16-17, 7 a. m. to 7 a. m 35.1 1.5 . 3.5 + 30.4 - 59.5 -29. 1 17-18, 7 a. m. to 7 a . m 35.4 1.4 3.7 + 30.3 - 49.2 -18.9 18-19, 7 a. m. to 7 a. m 35.4 1.5 3.5 + 30.4 - 15.7 +14.7 Total, 3 days 106. 2 4.4 10.7 4- 91.1 -124.4 -33.3 Average, 1 day 35.4 1.5 3.6 + 30.4 - 41.5 -11.1 Experiment Xo. 2U. Mar. 19-20, 7 a. m. to 7 a. m 43.8 1.5 3.7 + 3S.6 - 51.1 -12.5 20-21, 7 a. m. to 7 a. m 43.8 1.5 3.4 + 3S.9 - 11.5 ^27.4 21-22, 7 a.m. to 7 a.m 43.8 1.5 3.3 + 39.0 - 21.0 +18.0 Total, 3 days 131.4 4.5 10.4 +116.5 - 83.6 +32.9 Average, 1 day IS. 8 1.5 3.5 + 38. S - 27.9 +10.9 Table 79. — Gain or loss of protein (XXG.^o), fat, and water Xos. 23 and 24. Metabolism experiments Time. (a) Nitrogen gained ( + )or lost(-). (6) Protein gained (+) or lost (-), ax6.25. (c) Total car- bon gained (+)or lost (-). (d) Carbon in protein gained (+)or lost (-), bx0.53. (e) Carbon in fat, etc.. gained ( + )or lost (-), c-d. (/) Fat gained ( + )or lost (-), 6-5-0.761. 18S9. Experiment No. -23. Mar. 16-17, 7 a. m. to 7 a. m 17-18,7 a.m. to 7 a.m 18-19, 7 a. m. to 7 a. m Total, 3 days Average-, 1 day Experiment No. U Mar. 19-20, 7 a. m. to 7 a. m 20-21. 7 a. m. to 7 a. m 21-22, 7 a.m. to 7 a.m .... Total. 3 days . . Average, 1 day Grams. + .2 + .8 + .3 Grams. -0.6 -5.6 +1.3 Grams. + 5.1 + 8.5 + 4.6 Grams. 0.0 -3.0 Grains. + 5.1 + 11.5 + 3.9 Grams. + 6.7 + 15.0 + 5.1 -4.9 + 18.2 -1.6 +6.1 -2.3 + 20.5 ; + 26.8 + 6.9 +9.0 - .9 -5.6 i + 52.7 -3.0 + 55.7 + 72.8 + .5 +3.1 + 48.6 +1.6 - 47.0 + 61.5 +1.2 +7.5 + 38.3 ^4.0 + 34.3 + 44.8 +5.0 -1.7 +139.6 - 46.5 +2.6 + .9 +137.0 +179.1 + 45. 7 - 59. 7 70 Table 79. — Gain or loss of protein (NX6-86), fat, and water— Metabolism experiment* Not. :■; and 94— Continued. Tinie. (<7) Total hy- drogen gained ( + ) - Esti- mated energy of ma- terial oxi- dized in the bodv. a -(b+c +d+e). (SO Heat deter- mined. Heat deter- mined greater ( + )or less (— ) than esti- mated. u-f- (*) Heat deter- mined greater ( + )or less(-) than esti- mated, h+f. 1899. Experiment No. Mar. 16-17, 7 a. m. to 7a.m... 17-18, 7 a. m. to 7 a. m . . . 1^-19.7 a.m. to 7 a.m... 2,546 2,546 Calo- ries. 114 114 114 Calo- ries, ties. 140 j - 4 146 -32 137 j +8 ries. + 63 + 141 - 18 Calo- ries. 2,233 2.177 2 239 Calo- ries 2, 202 2,146 2,181 - 31 - 32 - 58 a nt. -1.4 -1.5 -2.6 Total. 3 davs 7,638 342 423 —28 |+ 252 6,649 2. 216 6 528 2. 176 -121 - 40 Average, 1 day Experiment No. .",. Mar. 19-20. 7 a. m. to 7a.m... 20-21 . 7 a. m. to 7 a. m . . . 21-22,7 a.m. to 7 a.m... Total. 3 days Average, 1 day 2,646 114 141 - 9 -1.8 ; ).", -32 - - &79 3,061 116 128 4-43 - 421 2,148 2, 214 2,353 2,216 + 9 - 26 +3.1 + .4 1.1 9,183 348 3,061 116 407 +29 +1,684 136 +10 ,+ 561 6,715 6,817 2,272 +102 + 34 +1.5 71 METABOLISM EXPERIMENT NO. 25. Suhjvft. — J. F. 8., a chemist, 29 years of age, weighing with under- clothing about 61 kilograms (111 pounds). Occupation during experiment. — Reading, writing, and miscellaneous observations with the apparatus, with as little muscular activity as was practicable. Duration. — Three days, beginning at 7 a. m. January 23, 1900. The usual preliminary period of 1 days duration preceded the metabolism experiment. The subject entered the calorimeter on the evening of January 22, thus spending four nights and three days in the respira- tion chamber. Diet. — Inasmuch as experiment No. 25 was the first with this sub- ject, the experiment was somewhat of a preliminary nature. The average food consumption of the subject had been determined by pre- liminary tests, and the diet was so arranged as to furnish approxi- mate^ the nitrogen and the energy it was believed the subject required. It was intended to make this the first of a series of three experiments, in which about one-fifth of the energy of the diet should be furnished by isodynamic amounts of different materials. Unfortunately an acci- dent to some of the machinery necessitated the stopping of the experi- ment shortly after the close of the first of the series of experiments. The diet consisted of a so-called basal ration furnishing about 110 grams of protein and 2,391 calories of energy, to which was added 63 grams of butter daily, furnishing 1 gram of protein and 505 calories of energy. The kinds and quantities of food served for each meal and the quan- tity of drink consumed at different periods of the day are as follows: Table 81. — Diet in metabolism experiment No. 25. FOOD. Food materials. Breakfast. Dinner. Supper. Total. Beef Grams. 30 35 300 90 25 10 Grains. 55 35 300 120 Grams. Grams. 85 Butter 28 400 90 25 35 10 98 Milk 1.C00 Bread 300 Parched cereal 50 Ginger snaps 35 70 Sugar 20 DRINK. Time. Water. Time. Water. Breakfast drams. 100 200 200 300 10.30 p. m Total for day Grams. 100 10.00 a. m 900 3.00 p. m 9.00 p. m 72 Daily routine. — The daily routine was essentially the same as that in previous experiments with other subjects, with the exception that the subject observed his pulse and body temperature at more frequent intervals. The general routine was as follows: Table 82. — Daily programme — Metabolism experiment No. 25. 6.50 a. m Take pulse and temperature. 3.00 p. m Drink 200 grams water. 7.00 a. m Pass urine, weigh self, weigh 6.00 p. m Supper. absorbers. 6.50 p. m Take pulse and temperature. 7.45 a. m Breakfast, drink 100 grams 7.00 p. m Pass urine, weigh self, weigh ab- water. sorbers. 10.00 a. m Drink 200 grams water. 9.00 p. m Drink 300 grams water. 12.50 p. m Take pulse and temperature. 10.20 p. m Take pulse and temperature. 1.00 p. in Pass urine. 10.30 p. m Drink 100 grams water, retire. 1.15 p. m Dinner. 1.00 a. m Pass urine. Table 83 summarizes the most important statistics in the diary kept b} T the subject. He weighed himself in underclothing twice each day. The reasons for not removing all the clothing in weighing, as was done in previous experiments, were two. It was desired to avoid the mus- cular work involved in dressing and undressing. It has also been found that the sudden increase of radiation of heat from the skin when the clothing was removed caused a decided rise of the temperature inside the chamber and thus disturbed the accuracy of the heat meas- urements to some extent. There was extremely little muscular exer- cise and no sensible perspiration. Hence the differences in weight from time to time may be considered to represent very nearly the actual changes in body weight. The determinations of pulse rate were made, of course, by the sub- ject himself, when either sitting or reclining, after several minutes' rest. The measurement at 6.50 to 7 a. m., however, was made just before rising. The body temperature was determined by means of a mercury ther- mometer. The temperature was, as a rule, taken in the axilla, although numerous sublingual observations were made. The thermometer was. as a rule, left in place ten minutes before reading. While the records of body temperature thus taken are probably not as accurate as might be desired, later observations with an electrical rectal thermometer since devised for continuous and accurate observations of internal body temperature a lead us to believe that the daily curves for the two are nearly parallel. In previous experiments a hygrometer had been placed in the chamber, and readings with dry and wet bulb were taken at frequent intervals. Inasmuch, however, as these readings were not used in the computations of results, and as it is desirable in rest experiments to a Arch. Physiol. [Pfliiger], 88 (1901), No. 9-10, p. 492. 73 avoid all unnecessary exercise, even that of rising and reading the h\ T grometer, these observations were not made in the experiments of 1900. Table 83. — Summary of the diary — Metabolism experiment No. 25. Weight of sub- ject in under- clothes. Pulse rate per min- ute. Temperature. Time. Weight of sub- ject in under- clothes. Pulse rate per min- ute. Temperature. Time. Axil- lary. Sublin- gual. °F. 98.3 97.9 98.3 98.7 97.8 Axil- lary. Sublin- gual. 1900. January 88. Kgs. 63.79 73 89 °F. 97.2 98.3 98.5 97. S •97.5 97.9 97.6 1900. Jan.2U— Cont'd. 12.30 p. m Kgs. 63 78 °F. °F. 97.7 1.52 p. m 9 05 a. m 1.55 p. m 98.5 I 2.25 p. m 98.3 98.4 98.6 98.6 98.8 98.5 98.3 98.3 98.2 11.30 a. m 2.45 p. m 3.23 p. ra 84 12 m 72 3.45 p. m 12.10 p. m 3.46 p. m 82 98.6 12.25 p. ni 97.7 98.3 98.0 98.8 98.7 98.1 97.9 98.3 4.10p.m 12.50 p. m . 4.30 p. m 75 69 4.55 p. m 2.35 p. m 5.15 p. m 75 2.40 p. m 78 86 82 5.30 p. m 69 3.50 r>.m .. 5.48 p. m 98.3 •5.57 p. m 71 5 p. m 73 6.49 p. m 98.5 6.53 p. m 77 5.35 p. m 6.57 p. m 7.30 p.m 65. 13 6.45 p. m. 75 78 98.2 64.63 7.38 p.m 98.2 98.4 98.3 98.2 97.4 98.1 98.3 98.6 98.2 98.4 97.6 97.7 8 p.m 98.2 98.1 8.30 p. m . . . 75 8.25 p. m 8.40 p. m 8.30 p. m 70 72 97.7 9.35 p.m...*. 68 97.7 97.7 97.6 97.7 9.21 p.m 10 p. m 97.5 97.5 10.10 p. m 10.10 p.m 10.15 p. m 10.25 p. m 69 67 83 10.20 p. m 64 97.0 10.20 p.m 97 January 2U. 6.55 a. m 8.30 a. m 64.21 10.25 p.m 64 71 .. 97.2 January 25. 8.40 a. m .*.. 7.03 a. m 7.38 a.m 64.49 9 a. m 98.6 98.6 78 9.25 a. m 78 98.4 9.30 a. m 8.37 a.m .. 86 9.35 a. m 8.50 a.m 98.5 9.55 a. m 98.2 97.9 97.2 98.3 98.2 8.53 a.m 82 10.30 a. m 68 80 74 84 81 69 98 5 10.40 a.m 9.37 a. m .. 80 11 a. m 98 3 11.25 a.m 10.29 a. m 76 11.35 a.m 10.40 a.m '98.0 11.55 a. m 10.45 a. m 71 74 12.17 p. m 10.54 a.m 74 Table 83. — Summary of the diary — Metabolism experiment No. 25 — Continued. Weight of sub- ject in under- clothes. Pulse rate per min- ute. Temperature. Weight Pulse rate per min- ute. Temperature. Time. Axil- lary. Sublin- gual. Time. ject in under- clothes. Axil- lary. Sublin- gual. 1900. Jan. 85— Cont'd. K 11 10 a. m o F 98.0 °F. 1900. January 26. K 6.55 a.m 70 °F. °F. 11.13 a. m 72 74 7 a.m 98. 1 11 9 5 a in 7.04 a.m j 64.43 8.33 a.m 11.35 a.m 98.1 90 11.39 a.m 72 69 8.39 a.m 88 90 8.41 a.m 12.09 p. m 98.0 8.42 a.m 97.9 98.5 72 68 68 9.26 a. m 12.27 p. m 9.30 a. m 91 12.45 p. m 9.72 a.m i 98.5 12.47 p.m 98.1 10 a.m 98.3 2 11 p m 81 98. 7 i 10.27 a. m 87 2.37 p. m 98.7 98.1 98.8 10.32 a. m 98.2 10.44 a.m i 98.2 81 11.01 a.m 98.2 98.1 97.9 97.7 97.9 97.9 3.19 p. m 11.12 a.m 3 26 p. m 82 11.30 a.m 3 28 p.m 98.0 98.3 98.4 98.5 11.33 a.m 82 97.9 3.31 p.m 11.55 a.m 3.33 p.m j 12.05 p.m ' 12.28 p.m 98.4 98.5 98.3 98.3 98.3 98. 2 12.34 p.m 72 76 4.10 p.m 12.51 p.m 75 ' 12.56 p.m 97.9 4.48 p. m 1.56 p.m 82 5.06 p. m 2.04 p.m 98.2 98.4 5.23 p. m 73 2.16 p.m 6.04 p.m 98.3 2.27 p.m 88 6.43 p.m 6.46 p.m 76 2.28 p.m 98.5 98.5 98.4 2.45 p.m 6.59 p. m 7.48 p.m 65. 19 3.07 p. m . . 92 98.4 3.08 p. m 98.6 7.51 p.m 7.55 p. m 69 3.12 p.m 93 97.9 3.21 p. m 98.5 98.7 98.7 98.6 8.22 p. m 97.7 3.33 p. m 98 8.46 p.m 97.8 ! 8.49 p.m 70 4 p.m 8.51 p.m 97.7 4.39 p.m 100 9.16 p. m 97.8 97.8 97.8 98.7 98.6 9.31 p.m 5.23 p.m 9.46 p.m 5.28 p.m 95 9.49 p.m 70 98.3 10.10 p.m 97.4 G.37 p.m 94 10.17 p.m 70 6.49 p.m . 98.3 10.20 p.m 97.2 1- 7 p. m 65.18 75 Detailed data of incorru and outgo. — The quantities of nutrients and energy in the daily diet in this experiment are shown in Table 84, and the total and daily elimination of matter and energy in the feces in Table 85, Table te— Weight, composition, and heat of combustion of foods — Metabolism experiment Xo. 25. Lab- ora- tory No. S165 3166 3169 3164 3168 3167 | Food materials. Weight per day. Water. Pro- tein. Fat. Car- ! bohy- j drates. Nitro- gen. Car- bon. Hydro- gen. Heat of combus- tion. Beef Butter Milk, skimmed. Bread Parched cereal Ginger snaps... Sugar Total Grams. 85 98 1,000 300 50 70 I 20 Grams. 53.4 8.6 894.0 115.8 2.8 8.4 Grams. 28.5 1.6 43.0 27.3 5.9 4.5 Grams. 2.8 84.4 4.0 6.3 .9 6.0 Grams. 51.0 146.7 39.o 54.9 20.0 Grams. 4.56 .25 6.80 4.38 .94 Grams. 16.68 62.10 47.60 84.12 21.10 30.91 8.42 Grams. 2.35 9.81 6.70 12. 09 2.97 4.49 1.30 Calories. 185 841 207 310 79 1.623 1,078.0 j 110.8 104.4 312.2 17.65 270.93 39.71 2, 896 Table 85. — Weight, composition, and Jteat of combustion of feces — Metabolism experiment Xo. 25. Lab- or a - torv No. Weight of feces. Water. Pro " Pat tein. * at - ■Shy- «£r drates. &eu - Car- bon. WvHrn Heat 0f gel °" combus- gen - tion. 3171 Total, 3 days Average, 1 day... Grams. 211.4 70.5 Grams. 145.8 48.6 Grams. 18.2 6.1 Grams. 8.0 2.7 Grams. Grams. 23. 4 2. 92 7.8 ! .9/ Grams. 29. 02 9.67 Grams. 4.27 1.42 Calories. , 332 111 The urine was collected and the nitrogen determined in the usual 6- hour periods each day. The usual aliquot portions of the urine during each period of the day were taken for the preparation of a composite sample for the day. The nitrogen and heat of combustion of this composite sample were determined. Aliquot portions of the urine for each day were taken for the preparation of a composite sample for the whole period of the experiment. Inasmuch as it was intended that this experiment should form a part of a series continuing for 9 days, a composite sample of the urine for the 3 days of experiment Xo. 25 was not made, but a corresponding sample for 5 days was prepared and analyzed in the usual manner. The statistics are shown in Tables 86 and 87. The quantities of carbon dioxid and water found in the ventilating air current during the different days of this experiment are shown in Tables 8S-90. Table 90 summarizes the calorimetric measurements during the experiment. 76 Table 86. — Amount, specific gravity, and nitrogen of urine by 6-hour periods — Metab- olism experiment No. 2-5. Date. Period. &SEL £££ Nitrogen content. 1900. Jan. 23-24 7 a. m. to 1 p. m 1 p. m. to 7 p. m 7 p. m. to 1 a. m . 1 a. m. to 7 a. m 24-25 25-26 26-27 ----- Total Total by composite. 7 a. m. to 1 p. m 1 p. m. to 7 p. m 7 p. in. to 1 a. m 1 a. in. to 7 a. m Total Total by composite. 7 a. ni. to 1 p. m 1 p. m. to 7 p. m Grams. Per cent. Grams. 281.1 1.027 1.40 3.94 314.6 1.029 1.60 5.03 201.5 1.032 2.03 4.09 178.0 1. 028 1.90 3.38 975.2 16.44 975. 2 L.0285 1.69 16.48 256. 6 380.3 225. 8 349.3 1,212.0 1,212.0 355. 2 430.7 1.026 1.032 1.027 1.016 1.022 1.019 1.51 1.27 1.79 1.06 1.09 1.08 3.87 4.83 4.04 3.70 16.44 16.48 3.87 4.65 7 p. m. to 1 a. m ... 303.8 1.021 1.34 4.07 1 a. m. to 7 a. m ... 461.1 1.014 ;82 3.7S Total 1.550.8 16. 37 Total by compos ite 1,5-50.8 1.019 1.07 16. 59 7 a. m. to 1 p. m ... 402. 6 1.019 .92 3.70 1 p. m. to 7 p. m ... 441.3 1.018 1.00 4.41 7 p. m. to 1 a. m ... 375. 6 1.017 1.0S 4.06 1 a. m. to 7 a. m ... 305. 6 1.019 1.12 3. 42 Total 1. 525. 1 15. 59 Total by com posite 1, 525. 1 1.0185 1.03 15. 71 7 a. m. to 1 p. m .. 331.8 1. 021 1.16 3.85 1 p. m. to 7 p. m .. 448.3 1.019 1.07 4. -0 7 p. m. to 1 a. m .. 405. 1.017 1.15 4.66 1 a. m. to 7a. m .. 280.3 1.020 1.24 3.47 Total 1,465.4 16.78 Total by com posite by composite 1.465.4 1.019 1.15 16. 85 Total, 5 (lavs G. 72a 5 1.21 8L41 Table 87. — Daily elimination of carbon, hydrogen, water, and energy in urine — Metab- olism experiment No. Date. of urine. Carbon. Hydrogen. Water. Heat of com- bustion. gSn, Totel - 1 1900. 1 I Grams. P.ct. Grams. IP. ct. Grams. P. d. Grams. - " lories. Jan. 23-24.. | 975.2 12.88 3.12 913.1 0.142 138.5 24-25.. 1,212.0 12.87 3.12 1, 150. .121 146.6 25-26.. 1,550.8 12.82 3.10 ■ 1,489.0 . 100 155. 1 26-27.. 1,525.1 12.21 2.96 1. 466. 2 .096 146.4 27-2^.. 5 days 1.4G5.4 13.14 3.18 1, 402. Total, 6,728.5 0.95 63. 92 0. 23 15.48 95.42 6.420.3 77 Table 88. — Comparison of residual amounts of carbon dioxid and water in the chamber at the beginning and end of each period, and the corresponding gain or loss — Metabolism expi riment No. 25. Date. 1900. Jan. 23 End of period < a. lp. 7 p. la. 1 p. 7 p. 1 a. 7 a. lp. 7 p. la. Total m Total Carbon dioxid. Total amount in chamber. Gram*. 27.-4 36.7 45.6 27. 6 26.6 Water. Gain ( + ) orloss(— ), Total amount of vapor over pre ceding P eriod - ch€ nber. remain- ing in Gain ( + ) orloss( — ) over pre- ceding period. Grami 4-9.3 + 8.9 -18.0 -1.0 Grams. 36.5 42.9 44.1 43.9 37.4! +6.4 +1.2 -6.5 .8 38. 6 44.6 26.0 24.9 +12.0 41.8 -6.0 39.3 -18.6 41.2 - 1.1 35.0 -4.4 -2.5 + 1.9 -6.2 -2.4 Change in weight of ab- sorbers, gain ( + ) orloss(-). Grami Total amount gained ( + ) or lost ( — ) during the period. Grams. +11 + 10 -11 -10 -17.4 -11.2 -11.2 -16.5 D. i .5 .1 8.2 m 35. 5 46. 7 26.8 26.4 | +10.6 -11.2 -19.9 - .4 40.3 43.5 40.3 36.5 +5.3 -3.2 -3.8 - 1 + 4.3 + 3.2 m - 3.2 m - 3.8 3 davs Total ! + 1.5 . +1.5 - 1 + .5 Total, - 1.0 . - 3 - 3.0 Table 89. — Record of carbon dioxid in ventilating air current — Metabolism experiment Xo. 25. Period. («) Ventila- tion (number of liters of air). Carbon dioxid. (h) Date. In incoming air. id) In out- going air. (e) Total excess in out- going air, d-c. Correc- tion for amount remain- ing in chamber. (9) Corrected amount exhaled by sub- ject, e+f. Total weight of car- (6) Per liter. (c) Total, a x 6. haled, g x &. 1900. Liters. Mg. Grams. Grams. Grams. Grams. Grams. Grams. Jan. 23-24 7 a.m. to 1 p.m ... 25, 652 0. 624 16.0 244.9 228. 9 + 9.3 238. 2 65.0 1 p.m. to 7 p. m ... 26,430 .593 15.7 247. 3 231.6 + 8.9 240. 5 65.6 7 p. m. to 1 a.m ... 27, 208 .561 15.3 226.6 211. 3 —18.0 193.3 52.7 1 a.m. to 7 a.m ... Total 7 a.m. to 1 p.m ... 26,430 .539 14.2 144.0 129.8 — 1.0 128. 8 35.1 105. 720 61. 2 862. 8 801.6 - .8 800.8 218.4 24-25 26, 430 .011 16.1 238. 8 222. 7 +12. 234. 7 64.0 1 p.m. to 7 p.m ... 27,208 .598 16.3 233. 4 217. 1 + 6.0 223. 1 60.8 7 p.m. to 1 a.m ... 27, 985 ..560 15.7 235. 219.3 -18.6 200.7 54.7 1 a.m. to 7 a.m ... Total 7 a.m. to 1 p.m ... 27,985 .549 15.4 150.7 135.3 - 1.1 134.2 36.6 109, 608 63.5 857.9 794.4 - 1.7 792.7 216. 1 25-26 27. 207 . 572 15.6 224. 8 209. 2 +10.6 219.8 59.9 1 p.m. to 7 p.m ... 26, 430 .588 15.5 233. 4 217. 9 +11.2 229. 1 62.5 7 p.m. to 1 a.m ... 27,985 .584 16.3 235. 6 219. 3 -19.9 199.4 54.5 1 a.m. to 7 a.m ... Total Total, 3 da vs. 27, 208 .548 14.9 156.6 141.7 - .4 141.3 38.4 108, 830 62.3 851 >. 4 788.1 - 1.5 789.6 215. 3 324, 158 187.0 2,571.1 2,384.1 - 1.0 2, 383. 1 649.8 78 Table 90. — Record of water in ventilating air current — Metabolism experiment No. 2 Period. (a) So a t —s a; = •- p > Water in in- coming air. Water in outgoing air. (flO S.S3 (h) £ be S-2 a O h I** lag gno O (i) 8 8^ °§* 111 o —' H Date. (b) c o (c) .0 X e 2 c (d) "■dg — 'J. N 3 d « < («) lie (/) + 3 o IB C a> o 1900. Jan. 23-24 7 a.m. to 1 p. m. 1 p. m.to 7 p. m. 7 p. m.to 1 a. m. 1 a.m. to 7 a. m. Total 7 a. m.to 1p.m. 1 p.m. to 7 p.m. 7 p. m.to la. m. 1 a. m.to 7 a. m. Total 7 a. m.to l.p. m. 1 p. m.to 7 p. m. 7 p. m.to 1 a. m. 1 a.m. to 7 a. m. Total Total, 3 days. Liters. 25, 652 26, 430 27, 208 26, 430 Mg. 0.877 .870 .862 .819 Grams. 22.5 23.0 23.5 21.6 Grams. 191.9 210.1 212.9 190.9 Grams. 46.6 37.8 42.3 36.2 Grams. 238. 5 247. 9 255.2 227.1 Grams. 216. 224. 9 231. 7 205.5 Grams. +17.4 + 11.2 -11.2 -16.5 Grams. 233.4 236. 1 220. 5 189.0 105, 720 90.6 805.8 162.9 968.7 878. 1 + .9 879.0 24-25 26, 430 27, 208 27, 985 27, 985 .887 .864 .834 .859 23.4 23.5 23.3 24.0 193.7 1 40.2 1 233.9 209.1 39.3 248.4 218. 5 43. 7 262. 2 189.7 ! 39.5 ' 229.2 210. 5 224. 9 238.9 205.2 + 5.4 - 1.5 - .1 - 8.2 215.9 223.4 238.8 197.0 109, 608 94. 2 811.0 162.7 j 973.7 879. 5 - 4.4 875.1 2.5-26 27,207 26, 430 27, 985 27,208 .853 .873 .841 .812 23.2 23.1 23.6 22.1 188.7 43.9 197.7 ' 37.0 199.7 43.3 181.8 36.3 232.6 234. 7 243.0 218.1 209. 4 211. 6 219. 4 196.0 + 4.3 + 3.2 - 3.2 - 3.8 213.7 214. 8 216. 2 192.2 108, 830 92. 767.9 160.5 928.4 836.4 + .5 836.9 321,158 276.8 2,384.7 486.1 2, 870. 8 2, 594. - 3.0 2,591.0 Table 91. — Summary of calorimetric measurements — Metabolism experiment i\ o. 25. Date. Period. (a) Heat meas- ured in terms of C 2 o- (6) Change of tem- perature of calo- rimeter. (c) Capacity correc- tion of calorim- eter, 6x60. id) Correc- tion due to tem- perature of food and dishes. (e) Water va- porized equals total amount exhaled less amount con- densed in chamber. (/) Heat used in vaporiza- tion of water, ex 0.592. (9) Total heat de- termined, a+c+d +/■ 1900. Jan 23 24 7 a. m.to 1 p.m... 1 p. m.to 7 p. m... 7 p. m. to 1 a. m . . . 1 a. m.to 7 a.m... Total 7 a. m.to 1 p.m... 1 p. m.to 7p.m... 7 p. m.to 1 a.m... 1 a.m. to 7 a.m... Total 7 a.m. to 1 p. m... 1 p. m.to 7 p.m... 7 p.m. to 1 a.m... 1 a. m.to 7 a.m... Total Total, 3 days. Calories. 588.0 490.6 447.1 248. 5 Degree. Calories. Calories. - 0.5 + 5.9 Grams. •2-2-2. 1 226. 1 231.5 199.0 Calories. 131.7 133.8 137.1 117.8 Calories. 719. 2 -0.01 -0.6 621.6 590. 1 366.3 1,774.2 - .01 j -0.6 + 3.2 879.0 520.4 2, 297. 2 24-25 542. 2 467. 2 443.7 239. + 0.3 - 3.0 + 6.1 214.9 222.4 240. 8 199.0 127.2 131.7 142. 6 117.8 669.7 595.9 592.4 + .04 +2.4 359. 2 1,692.1 + .04 +2.4 + 3.4 877. 1 519.3 2,217.2 25 26 520. 2 495. 7 430. 2 270.9 + 0.2 - 2.2 + 6.6 214.7 214.8 216. 2 192.2 127. 1 127. 2 128.0 113.8 647.5 620.7 564.8 + .03 +1.8 386.5 1,717.0 + .03 1 +1.8 + 4.6 837.9 496.1 2,219.5 5, 183. 3 +3.6 +11.2 2,594.0 ] , 535. 8 6, 733. 9 79 Balance of income and outgo of matter and energy. — Tables 92-95 summarize the income and outgo of nitrogen, carbon, h} T drogen, and energy. Table 92.— Income and outgo of nitrogen and carbon — Metabolism experiment No. 25. Nitrogen. Carbon. Date. (a) In food. (6) In feces. (C) In urine. (d) Gain ( + )or loss ( — ) a— (b+c). (e) In food. (/) In feces. (9) In urine. (h) In respira- tory prod- ucts. Gain ( + )or loss ( — ) €~(f+ g+h). 1900. Jan. 23-24, 7 a. m. to 7 a. m. . 24-25, 7 a. rn. to 7 a. m. . 25-26, 7 a. m. to 7 a. m . . Grams. 17.7 17.6 17.7 Grams. 1.0 .9 1.0 Grams. 16.4 16.4 16.4 Grams. +0.3 + .3 + .3 Grams. 270.9 270. 9 270.9 Grams. 9.7 9.6 9.7 Grams. 12.9 12.9 12.8 Grams. 218. 4 216.1 215. 3 Grams. +29.9 +32.3 +33.1 Total, 3 days 53.0 2.9 1.0 49.2 16.4 + .9 + .3 812.7 29.0 38.6 12.9 649.8 -+-95.3 Average, 1 day 17.7 270. 9 9.7 216.6 +31.7 Table 93. — Income and outgo of water and hydrogen — Metabolism experiment No. 25. Water. Date. (a) In food. (6) In drink. (c) In feces. (d) In urine. (e) In respiratory products. (/) Apparent loss, a+b- (c+d+e). 1900. Jan. 23-24, 7 a. m. to 7 a. rn 24-25, 7 a. m. to 7 a. m 25-26, 7 a. m. to 7 a. m Grams. 1,078 1,078 1,078 Grains. 900 900 900 Grams. 48.6 48.6 48.6 Grains. 913.1 1, 150. 1,489.0 Grams. 879.0 875.1 836.9 Grams. + 137.3 - 95.7 -396. 5 Total, 3 davs 3, 234 1,078 2, 70G 145.8 3,552.1 2.591.0 1,184.0 863.7 -354.9 900 48.6 -118.3 ' Hydrogen. Date. (9) (h) In In food. feces. In urine. (0 Apparent gain, 9-{h+i). (m) Loss from water, /-h 9. (») Total gain( + ) or loss (— ), l+m. 1900. Jan. 23-24, 7a.m.to7a.ra 24-25, 7 a. m. to 7 a. m 25-26, 7 a. m. to 7 a. m Grams. \ Grams. 39.7 1.4 39.7 1.4 39. 7 1. 5 Grams. 3.1 3.1 3.1 Grams. + 35.2 + 35.2 + 35.1 Grams. +15.3 -10.6 -44.1 Grams. +50. 5 +24.6 - 9.0 Total, 3 days 119.1 i 4.3 39.7 ! 1.4 9.3 3.1 +105.5 + 35.2 +39.4 +13.2 +66.1 +22.0 80 Table 94. — Gain or lo88qfprotein(NX6.25),fat } and water — Metabolism experiment No. 25. Date. (a) (b) (c) (rf) (e) Protein Total Carbon in Carbon in F Itrogen W}* A?™ protein fat, etc., LJX*,, ined(+) ? ain ed( + ) c*™ ^ gaill ed(+) gained( + ) ^ ain ^ d ^ J?f , lost(-), or , «* . ww x ! lost(-), Ni„_ gained or 10St( '• ox 6.25. or lost (- , oxO.53. lost(-), c-d. lost e* 0.761. 1900. Jan. 23-24. 7 a. m. to 7 a. m 24-25. 7 a. in. to 7 a. in 25-26. 7 a. m. to 7 a. m Total. 3 days Average. 1 day Gram. Grams. Grams. Grains. Grams. Grams. +0.3 1 4-1.9 -29.9 -1.0 +28.9 + 38.0 - .3 +i.8 : +32.3 +1.0 -31. 3 - 41.1 - .3 -1.9 +33. 1 +1.0 +32.1 4- 42.2 - .9 +5.6 i-95.8 -3.0 +92. 3 -121.3 + .3 +1.9 +31.8 +1.0 -30. 8 - 40.4 Date. 1900. Jan. 23-24. 7 a. in. to 7 a.m. 24-25. 7 a. m. to 7 a. in. 25-26, 7 a. m. to 7 a. in. Total. 3 days Average. 1 day (9) Total hydrogen gained( + ) or lost ( — ). (h) Hydrogen in protein gained ( or lost C - 6x0.07 (0 Hydrogen in fat (*) Hydrogen in water, etc., } orfoSY-V Sained' ( + ' 0r ,l nV, ( fi } ' orlost(-) /x 0.118 ) 9-(h+i). Grams. +50.5 -24. 6 - 9.0 -66.1 -22.0 Gram. +0.1 + .1 - .2 Grams. Grams. - 4.5 +45.9 + 4.8 -19.7 + 5.0 -14.2 (0 Water gained (+) or lost (-), Jrx9. - .4 - .1 14.3 - 1.8 +51.4 -17.1 Grams. -413.1 -177.3 -127.8 -462.6 -1-54.2 Table 95. — Income and outgo of energy — Metabolism experiment No. 25. Date. (a) Heat of com- bustion of food eaten. 1900. Jan. 23-24, 7 a. m.-to 7 a. in 24-25, 7 a. m. to 7 a. m 25-26,7 a.m. to 7 a. m Total, 3 days Average, 1 day Calo- ries. 2,896 2,896 8,688 2,896 (6) (c) Heat of Heat of com- com- bustion bustion of of feces. ' urine. Calo- rics. Ill 110 111 Calo- ries. 138 147 155 (d) Esti- mated heat of com- bustion of (e) Esti- mated heat of com- bustion ESS *& gained ( + ) or lost (-)• I ( + ) or lost (-)• (/) Esti- mated energy of ma- terial oxi- dized in the , body, i a-(6+c d+e). (9) Heat deter- mined Caln- I >'.s. +11 +10 +11 332 111 440 147 +32 -11 Calo- ries. + 362 - 392 + 403 (h) Heat deter- mined greater I ( + )or less(-) than esti- mated, OS- Calo- ries. 2, 274 2,237 2,216 + 1,157 I 6,727 2. 242 Calo- ries. 2,297 2,217 2,220 Calo- ries. +23 -20 - 4 6.734 + 7 2,244 - -1 (i) Heat deter- mined greater (+)or less(-) than esti- mated, h+f. Perct +1.0 - .9 + .2 - .3 + .1 METABOLISM EXPERIMENTS NOS. 26 AND 28. Subject. — J. F. 8-, as in the previous experiment, weighing with underclothing about 61 kilograms (141 pounds). Occupation during > xpt riment. — Reading, writing, and miscellaneous observations within the apparatus, with as little muscular activity as practicable. 81 Duration. — Experiments Nos. 26 and 28 form the first and last of a series of 3 experiments which were made for the purpose of studying the relative replacing power of isodynamic amounts of different mate- rials; the intermediate experiment is not reported here. The usual preliminary period of ± days duration began with breakfast February 10, 1900, and ended with supper February 13. The subject entered the calorimeter on the evening of February 13 and experiment No. 26 began at 7 a. m. February 14, continuing 3 days. Experiment No. 28 began at 7 a. m. February 20, and continued until 7 a. m. February 23. Diet. — The diet consisted of a basal ration furnishing about 99 grams of protein and 1,982 calories of energ}^ per day. To this, in experiment No. 26, was added 63.5 grams of butter, furnishing 1 gram of protein and 508 calories of energy. In experiment No. 28 the material added consisted of 128 grams of cane sugar, furnishing 507 calories of energy per day. The protein and energy were thus prac- tically the same in both of the experiments. The kinds and quantities of food in the basal ration as served for each meal, the character and amount of the supplemental ration in the different experiments, and the quantity of drink consumed at different periods of the day in each experiment were as follows: Table 96. — Diet in metabolism experiments Nos. 26 and 28. FOOD— BASAL RATION. Food materials. Breakfast. Dinner. Supper. Total. Beef Grams. 35 10 300 50 Grams. 50 12 400 100 30 Grams. Grams. 85 Butter 8 300 50 30 25 30 Milk 1,000 200 Bread Ginger snaps 60 Parched cereal 25 15 50 Sugar 15 FOOD-SUPPLEMENTAL RATION. Experiment No. 26 63.5 grams butter per day. Experiment No. 28 128 grams sugar per day. DRINK. Time. Water. Grams. 100 200 200 Time. Water. Breakfast 9 p. m Grams. 300 10 a. m Total for dav 800 3 p. m 13007— No. 109—02- 82 Daily routine. — The general routine of the experiment was as follows: Table 97. — Daily programme — Metabolism experiments Xos. 26 and 28. 6.50 a. in Take pulse and temperature. 6.00 p.m Supper. 7.00 a.m Rise, pass urine. weigh self. weigh 6.50 p.m 7.00 p.m Take pulse and temperature. absorbers. Pass urine, weigh self, weigh ab- 7.45 a.m Breakfast, drink 100 grams water. sorbers. 10.00 a. m Drink 200 grams water. 9.00 p. m Drink 300 grams water. 12.50 p. m Take pulse and temperature. 10.20 p.m Take pulse and temperature. 1.00 p. m Pass urine. 10.30 p. m Retire. 1.15 p.m Dinner. 1.00 a.m Pass urine. 3.00 p. m Drink 200 grams water. The more important statistics in the diary kept by the subject dur- ing experiments Xos. 26 and 28 are summarized in Table 98. Table 98. — Summary of the diary — Metabolism experiments Xos. 26 and 28. Time. Weight Pulse of subject rate per in under- 1 min- clothes. ute. Temper- ature. Time. Weight of subject in under- clothes. Pulse rate per Temper- min- ature. ute. 1900. Experiment No. 26. Feb 14. 7.00 a.m Kge. 64 68 78 67 64 : F. 97.8 98.3 98.1 97.8 97.9 98.5 98. 5 98.5 1890. ExpLNo.2&— Cont'd. Feb. 15. 5.30 p. m Kgs. 98.0 8.36 a. m 5.49 p. m 6.30 p. m 69 69 68 75 67 10.27 a. m 98.2 12.27 p. m 6.55 p. m 7.30 p. m 64.87 98.2 12.33 p. in 98.1 12.53 p. m 61 8.30 p. m 97.6 1.00 p.m 8.54 p.m 2.27 p m . . 77 9.00 p.m 97.5 3.47 p. m 9.30 p. m.... 67 4.30 p. m... 72 67 9.35 p. m ^7.4 5.30 p. m... 9.51 p. m 97.6 98.7 10.15 p. m 70 6.17 p.m 8.13 p.m... 64.88 10.20 p. m 97.5 97.6 97.5 97.7 Feb. 16, 6.55 a. m 71 82 8.30 p. m 64 64 64 69 7.00 a. m 8.32 a. m 64.01 98.1 9.29 p. m... 8.40 a. m 98.3 Feb. 15, 6.50a.m... 98.1 9.30 a.m 9.37 a. m 79 700a m 64.18 98.2 78 10.31 a.m 76 72 98.3 7.39 a m 98.3 98.5 11.26 a. m 8.33 am.. 82 80 11.30 a. m 98.2 9 28 a m 12.27 p.m 70 9 30 a. m 98.3 12.30 p. m 98.1 10.33 a m 71 12.58 p. m 71 10.46 a m . 98.5 98.1 1.00 p. m 98.2 11.30 a. m... 70 68 2.01 p. m 80 79 81 98.2 12.31p.m... 2.30 p. m 9S.2 12 37 p m 9S.4 3.35 p.m 98.3 12 54 p m 68 98.2 1 00 p. m 98.2 98.2 98.5 4.27 p. m 79 1.59 p m 75 81 77 76 4.30 p. m 98.2 2.28 p. m.. 5.30 p. m 75 80 98.5 3.35 p. m 5.43 p. m 6.32 p. m 98.7 4.28 p m 4.30 p. m 98.1 6.42 p.m 98.4 '83 Table 98. — Summary of the diary — Metabolism experiments Xos. 26 and 28 — Continued. Time. Weight Pulse of subject rate per in under- ruin- clothes, ute. Temper- ature. Time. Weight Pulse of subject rate per Temper- in under- min- ature. clothes. ute. 1900. Experiment No. 86. Feb. 16, 7.00 p. m Kgs. 04. 73 77 75 °E. 98.5 1900. Expt. No. 98— Cont'd. Feb. 21. 1.54 p. m Kf/s. 3 °F. 2.01 p. m 98.2 7.40 p. m 9S 98.3 97.8 97.8 "'"' l 93 2.34 p. m 9S.5 8.26 p. m 8.30 p. m... 71 3.52 p. m S6 98.2 9.31p.m.... Experiment N . 18. Fel>. 20. 6.55 a. m 68 72 88 4.35 p. m 76 79 75 77 79 9^.2 5.28 p. m 98.5 7.00 a. m 63. 71 98. 1 6.32 p. m 7.32 a. m 6.59 p. m 98.2 98. 4 7.00 p. m .... .64.63 8.30 a. m 91 81 97.7 8.31 a. m 98. 4 98.7 98.4 98. 2 97.9 9.30 a. m 99 84 81 78 70 8.27 p. m 79 10.30 a. m 8.30 p.m 97.7 11.30 a. m 9.27 p.m 73 11.36 a. m 97.6 12.27 p. m j Feb. 22, 6.55 a. m 69 So 12.33 p. m ' 98.1 7.00 a. m 63. 85 98. 1 70 12.59 p. m 98. 1 7.40 a. m 97.9 81 8.28 a. m 93 98.3 98. 2 9^.1 8.33 a. m 98.3 3.34 p. m 81 79 71 9.°7 a. m 95 4.30 p. m 9.30 a. m 98. 2 5.32 p. m 10.29 a. m.... 10.30 a. m 87 5.41 p. m 98 9<.4 6.35 p. m 77 11.36 a. m 82 74 6.40 p. m 98.1 12.27 p. m .... 72 12.30 p. m 98. 2 7.00 p. m 7.30 p. m 64. 32 98.1 97.8 70 12.58 p. m 98 2 8.28 p. m 2.07 p. m 83 8.30 j». m... 97.7 98.6 9.30 p. m... 67 2.30 p. m 84 9.32 p. m 97.3 2.50 p. m 98. 4 10.18 p. m 67 3.30 p.m 84 78 73 98. 4 10.20 p. m. 97.2 4.29 p. m 98.4 Feb. 21, 6.55 a. m 73 87 5.30 p. m 7 00 a. m... 63.83 98.1 98.2 7.29 a. m 6.30 p. m 73 76 74 98.4 7.30 a. m 98.1 98.3 98.3 6.57 p. m 7.27 p. m 64.77 98.1 8.29 a. m. 92 8.30 a. m 7.31 p.m 97.7 9.30 a. m 101 87 8.32 p.m 72 10.30 a. m 1 8.42 p. m 97.5 10.33 a. m \ 98.4 9.27 p.m 66 11.27 a. m 1 78 97.3 11.31 a. m 1 97.9 98.1 10.20 p.m 70 76 97.1 12.31 p.m 1 77 73 Feb. 23, 6.55 a. m 1.00 p. m ' 7.00 a. m 64.05 98 1 84 Detailed data of income and outgo. — The quantities of nutrients in the basal ration which were used for the experiments and the quantities in the supplemental ration in the two experiments are shown in Table 99. The elimination of matter and energy in the feces was determined in each experiment and the results are recorded in Table 100. Table 99. Weight, composition, and heat of combustion of foods — Metabolism experiments Nos. 26 and 28. Lab- ora- tory No. Food materials. Weight WatPT per day. Uater ' Pro- tein. Fat. Carbo- hy- drates. Nitro- gen. Car- bon. Hydro- gen. Heat Of com- bus- tion. 3176 Beef Grams. Grama. 53. 1 30. 3. 1,000.0 900.0 200.0 78.fi Grams. 28.7 Grams. 2.4 Groins. 47.0 97. - 47.9 39.5 15.0 Grams. Grams. 4.60 16.62 'Trams. 2.30 3.01 6.30 3.97 2.97 .97 Colo- 187 3177 Butter .5 42.0 17.8 3.7 5.9 25.8 3.0 3.2 5.0 .9 .08 6.70 2.84 .60 .94 19. 51 46.30 55. 52 26.59 21.10 6.31 240 3179 3180 Milk, skimmed Bread 462 561 3181 3168 Ginger snaps Parched cereal Sugar Total basal ration.. EXPERIMENT NO. 26. Butter (supplemen- tal ration) Total ration, 1 day. EXPERIMENT NO. 28. Total basal ration Sugar (supplemen- 60.0 50.0 15.0 2.5 2.8 266 207 59 3177 1,440.0 1,040.0 63.5 6.3 I 98.6 1.0 40.3 54.5 247. 2 15. 76 .16 191. 95 41.29 27.50 6.36 1,982 .508 1,503.5 1,046.3 09.6 94.8 247.2 15.92 233.24 33.86 2.490 1,440.0 128.0 1,040.0 98.6 40.3 247. 2 128.0 15.76 191.95 53. SS 27.50 8.29 1,982 507 Total ration, 1 day. 1,668.0 1.040.0 98.6 40.3 , 375.2 15.76 245.83 i 35.79 2,489 Table 100. — Weight, composition, and heat of combustion of feces — Metabolism experiment* Nos. 26 and 28. Lab- ora- tory No. Weight ' Pro- <**?■ of feces. Water tern. F ^ ^ Nitro- gen. Car- bon. TT,. Hrri Heat of gen combu *- &eu - tion. 3183 Experiment No. 26. Total, 3 days Average, 1 day... Experimenl No. 25. Total, 3 days Average, 1 day... Grams. Grama. 286.5 171.0 78.8 57.0 Grams. Grams. Grams. 20. 6 8. 5 20. 1 6.9 ' 2.8 6.7 Grams. ! Grams. 3.26 28.33 1,09 9.44 Grams. Calories. 3. 41 317 1.14 106 3185 219.9 155.2 23.3 12.1 ; 16.1 73.3 51,7 7.8 4.0 5.3 0. 71 29. 93 1.25 9.98 4.02 335 1.34 112 The usual statistics of outgo of nitrogen, carbon, hydrogen, water, and energy in the urine are given in Tables 101 and 102. The urine was collected in the usual periods for twenty-four hours following the close of experiment No. 28. The elimination of nitrogen in these periods was 3.48, 5.25, 4.74, and 2.96 grams, respectively, or a total of 16.43 grams. 85 Table 101. — Amount, specific gravity, and nitrogen of urine- 26 and 28. Metabolism experiments Nos. Date. Period. Amount of urine. Specific gravity. Nitrogen content. 1900. Feb. 14-15 Experiment No. 26. 7 a. ni. to 1 p. m. Grams. 326. 7 297. 6 264. 8 327. 4 1.0225 1. 0245 1.0225 1.017 Per cent. 1.31 1.57 1.54 1.10 Grams. 4.28 1 p. m. to 7 p. m 4.67 7 p. m. to 1 a. in 4.08 1 a. m. to 7 a. m 3.60 Total . 1,216.5 16.63 Total by composite 7 a. m. to 1 p. ni 1, 216. 5 1.021 1.38 16. 7^ 15-16 451.4 356.7 274.3 443.7 1.017 1.021 1.021 1.0115 .83 1.23 1.35 .73 3. 75 4.39 3.70 3.24 Total 1,526.1 1,526.1 15. 08 1.0175 .99 15. 11 16-17 381.6 400.2 266.2 292.4 1. 0185 1. 0185 1.020 1.017 .91 1.04 1.35 1.10 3.47 4. 16 3.59 3.22 Total . 1. 340. 4 1,340.4 14.44 1.0185 1.09 14.61 4,083.0 4, 083. 46. 15 46.00 Experiment No. 28. 7 a. in. to 1 p>. m Feb 20-21 379.2 327. 3 207. 1 2.56. 2 1.0185 1.021 1.0255 1.018 1.12 1.39 1.82 1.30 4. 25 1 p. m. to 7 p. m 4.55 3.77 3.33 Total 1,169.8 1, 169. 8 15. 90 Total b v composite 1.020 1.37 16.03 21-22 340.7 267. 7 276.8 407.0 1.018 1.024 1. 020 1.011 1.09 1.60 1.41 .82 3.71 4. 28 7 p. m. to 1 a. in 3.90 1 a. m. to 7 a. m 3.34 - Total . 1, 292. 2 1, 292. 2 15. 23 Total bv composite 1.017 1.19 15. 38 7 a. m. to 1 p. m 22-23 302. 8 258.5 234.7 408.5 1.0185 1.023 1.022 1.012 i.n 1.58 1.62 .84 3.36 4.08 3.80 3.41 Total 1,202.5 1, 202. 5 14. 65 Total bv composite 1.018 1.23 14.79 Total . 3 da vs, b v periods 3.664.5 3, 664. 5 45. 78 Total bv composite | 46.20 Total, 9 days, 8 by composite 11, 998. 8 1.019 1.19 138. 84 'Including 3 days of an experiment not here reported. 86 Table 102. — Daily elimination of carbon, hydrogen, water, and erxrgy in urine — Metab- olism experiments Xos. 26 and 28. Date. p . -, Amount fenoci. Qf urine _ Carbon. W Heat of com- bustion. gr P am. Total - 1900. Feb. 14-15 15-16 16-17 Experiment No. 26. Q ramSm 7 a.m. to 7 a.m...: 1,216.5 7 a.m. to 7 a.m... 1,526.1 7 a.m. to 7 a.m... 1,340.4 P.ct. Grams.' P.ct. 11.93 10.82 10.36 Grams. 2.87 2.61 2.50 P.ct. Grams. Calorie. 1. 157. 3 0. 103 1.472.4 .082 1,289.0 .101 Oalorit s 125 125 135 Total, 3 days.. 4,083.0 33.11 7.98 3,918.7 385 Feb. 20-21 21-22 22-23 Experiment No. 88. 7 a.m. to 7 a.m... 1,169.8 7 a.m. to 7 a.m... 1,292.2 7 a.m. to 7 a.m... i 1,202.5 11.40 10.92 10.51 2.75 2.63 2.53 1,113.2 .102 1,238.0 .103 1,1.50.3 .110 119 133 132 Total, 3 days.. 1 3,664.5 32.83 7.91 3,501.5 384 Total.9days«. 11,998.8 0. S3 1 99. 59 0. 20 24.00 | 95.88 11,. 504. 5 .095 1,141 a This period includes the 6 days of experiments Nos. 26 and 28 and 3 days of an intervening experi- ment not reported here. ^7 Tables 103-105 show the quantities of carbon dioxid and water found in the ventilating air current in these two experiments. Table 103. — Comparison of residual amounts of carbon dioxid and water in the chamber at th> beginning and end of each period, and the corresponding gain or loss — Metabolism riments Nos. 96 and 28. Carbon dioxid. Water. Date. End of period. Gain - Total or loss \ - ) amount in over chamber, preceding period. Total c ■ , Total amount of ^,^.,\ amount vapor orl ^-i;-' gained (+) remaining r . rt _^ il1tT or losl in cham- P^Sf 8 during the ber. period. period. 1 1900. Feb. 14 14 14 15 15 jLxptruntm jvo. -,'o. 7 a. m Grams. 22. 2 32. 3 35. 7 22. x 25. 3 Grams. Grams. 36.3 . 36.9 38.4 35.5 33.6 Grams. Grams. 1 p.m 7 p. m 1 a.m +10. 1 - 3.4 -12.9 - 2.5 - 0.6 - 1.5 - 2. 9 - 1.9 - 0.6 - 1.5 - 2.9 - 1.9 Total 1 p.m 7 p.m - 3.1 . — 2.7 07 15 15 34.4 34.8 24. 1 - 9.1 - 0.4 -10.7 - 1.5 37.4 36. 9 34. 8 32.3 — 3. ;*• - 0.5 - 2.1 — 2.5 + 3. v - 0.5 16 16 1 a.m 7 a.m Total 1 p.m - 2.1 - 2.7 . - 1.3 - 1.3 16 16 30. *> 39. 5 21.5 23. + 8.7 -18.0 - 1.5 35.9 38.8 34.4 31.4 - 3.6 - 2.9 - 4.4 - 3.0 -3.6 - 2.9 17 17 1 a.m , a. m Total Experiment No. . - - 4.4 - 3.0 - 0.4 . - 0.9 - 0.9 Feb. 20 26.4 35.2 37. 2 24.1 24.1 + 8.8 - 2.0 -13.1 32.9 . 39.9 40.1 34. 8 30. 8 - 7.0 - 0.2 - 5.3 - 4.0 20 20 21 1 p.m - 7.0 - 0.2 - 5.3 21 7 a.m Total... - 4.0 - 2.3 . - 2.1 2 1 l p.m 7 p.m 21 21 38.0 19.0 26. 4 -13.9 -19.0 - 7.4 — 2. 5 40.3 30.2 36.1 35. 5 - 9. 5 -10.1 - 5.9 - 0.6 -r 9.5 -10.1 5 9 22 Total 1 p. m 7 p.m 1 a. m Total - 0.6 + 4.8 - 4.7 -4.7 •22 22 23 23 36.7 39. 7 26. 28, 7 - 3.0 -13.7 39.3 36.5 33.4 — 3. 3 + 0.5 — 2. y — 3.1 - 3.3 + 0.5 - 3.1 - 0.2 - 2.1 - 2. 1 »The differences in weight of the absorbers were so small as to be within the limit of error of the weighing apparatus. There was no drip. 88 Table 104. — Record of carbon dioxid in ventilating air current — Metabolism experirnenU Xos. 26 and 28. Date. 1900. Feb. 14-15 15-16 16-17 Feb. 20-21 21-22 Experiment No. 26. 7 a. m. to 1 p. m . . . 1 p.m. to 7 p. in ... 7 p. m. to 1 a. in . . . 1 a. m. to 7 a. m . . . Total 22-23 7 a.m. to 1 p. m 1 p.m. to 7 p.m 7 p.m. to 1 a. in 1 a.m. to 7 a.m Total 7 a. m. to 1 p. m 1 p. m. to 7 p.m 7 p. m. to 1 a. m 1 a.m. to 7 a.m Total Total, 3 days Experiment No. 28. 7 a.m. to 1 p.m ... 1 p. m.to7 p. m ... 7 p.m. to 1 a.m ... 1 a. m. to 7 a. m ... (a) Carbon dioxid. In incoming Ventila- air. tion (number {b) (c) of liters of air] Liters. 28,762 28,762 30. 316 Per liter. Total, axb. Mg. Grams. 0.529 15.2 .568 16.3 .568 17.2 (d) In out- going air. 28,762 .555 I 16.0 Grams. 212. 1 215.9 204.1 144.2 (0 Total excess in out- going air, d-c. Grams. 1 196.9 199.6 186.9 128.2 116, 602 64. (/) Correc- tion for amount remain- ing in chamber. 776.3 ■11.6 Grams. +10.1 + 3.4 -12.9 + 2.5 Cor- rected amount exhaled by sub- ject. e+f. (A) Total weight of car- bon ex- haled, <7X r 3 T . Grams. 207.0 203. 174.0 130.7 Grams. 56.5 55.4 47.4 35.6 + 3.1 714. 27, 985 . 556 29. 541 . 561 30, 316 . 550 30, 316 . 555 15.6 16.6 16.7 16.8 220. 226.7 204.0 145.2 118, 158 .1 65.7 795.' 29,540 .582 17.2 209.2 30,316 .571 ! 17.3 225.4 29,540 .551 16.3 202.6 30,316 .559 16.9 144.8 119, 712 782. 204. 4 210. 1 187.3 128.4 730. 2 + 9.1 + .4 -10.7 - 1.5 213. 5 210.5 176. 6 126. 9 2.7 727. I 192.0 208.1 127.9 + 8.2 + 8.7 -18.0 + 1.5 200.2 216. 8 168.3 129.4 ! 714.3 ■14. 3.54,472 198.1 27, 985 27,208 28, 762 28, 762 .572 .576 .578 . 557 16.0 15.7 16.6 16.0 2,354.2 i2,156.1 2. 156. 9 240.0 231. 6 214. 1 1.54. 6 224. 215.9 197.5 138.6 + 8.8 + 2.0 -13.1 232.8 217.9 184.4 138.6 Total 112,71' 64.3 i 840.3 776.0 2.3 773.' 7 a.m. to 1 p.m 1 p.m. to 7 p.m 7 p.m. to 1 a.m 1 a.m. to 7 a.m Total 7 a.m. to 1 p.m 1 p. m. to 7 p. m 7 p.m. to 1 a.m 1 a.m. to 7 a.m Total Total. 3 da vs. 26,430 .614 16.2 231.0 214.8 + 7.8 222.6 27,208 .609 16.6 225. 208.4 | + 3.0 211.4 28, 762 .560 16.1 220.2 204.1 -13.7 190.4 28, 762 .609 17.5 154.4 136.9 [ + 2.7 139.6 111, 162 66.4 830.6 764. 2 - .2 764.0 332. 709 198.3 2, 514. 2 2, 315. 9 + 2.3 2,318.2 194.9 58.2 •V7.4 48.2 34.6 198.4 54.6 59.1 45.9 35.3 194.9 588. 2 63.5 "-.9. 4 .50.3 37.8 211.0 26, 430 .64U 16.9 229. 6 212.7 +13.9 226. 6 61.8 27,208 .637 17.3 235. 5 218. 2 -19.0 199.2 .54.3 28, 762 .597 17.2 227. 6 210.4 + 7.4 ! 217.8 59.4 26, 430 .612 16.2 150.6 134.4 + 2.5 • 136.9 37.3 108, 830 67.6 : 843. 3 775. 7 + 4.8 780.5 212. 8 60.7 57.6 51.9 38.1 208. 3 632.1 89 Table 105. — Record of water in ventilating air current- and 28. Metabolism experiments Nos. Period. (a) £^ 11 ll BIS ll Water in in- coming air. Water n outgoing air. (9) £ to I'l -1 - Correction f o r ^ water remaining > in chamber. O 9) . !» Date. 0) (c) X e (d) C 5c n S c o O y p < (c) fl « S3 I SB < Grams. if) + 1900. Experiment No. 26. Liters. Mg. Gravis. Grams. Grams. Grams. Grams. Grams. Fell. 11-15 7 a.m. to 1 p. m . 28, 762 0.816 23.5 197.3 47.6 244.9 221.4 + 0.6 222. 1 p. m. to 7 p. m . 28, 762 .852 24.5 196.1 42.2 238. 3 213.8 + 1.5 215. 3 7 p.m. to 1 a. m . 30, 316 .828 25.1 184.6 49.0 233.6 208.5 - 2.9 205. 6 1 a.m. to 7 a.m. Total 28, 762 .833 24.0 171.3 40.8 212.1 188.1 - 1.9 186.2 116, 602 97.1 749.3 179. 6 928.9 831.8 - 2.7 829. 1 7 a. m. to 1 p. m . 15-1(3 27,985 .867 24.3 179.7 42.7 222. 4 198.1 + 3.8 201. 9 1 p.m. to 7 p.m. 29. 541 .890 26.3 195.0 43.0 238.0 211. 7 - .5 211.2 7 p. m. to 1a.m. 30, 316 .860 26.1 185. 5 47.6 233. 1 207. - 2.1 204.9 1 a.m. to 7 a. m . Total 30, 316 .828 25.1 174.1 42.1 216.2 191.1 - 2.5 188.6 118, 158 101.8 734.3 175.4 909.7 807.9 - 1.3 806.6 7 a. m. to 1 p. m . 16-17 29, 540 .828 24.5 182. 3 44.7 227. 202.5 + 3.6 206.1 1 p. m. to "p.m. 30, 316 .820 24.9 188.8 43.0 231.8 206.9 + 2.9 209.8 7 p. m. to 1 a. m . 29, 540 .824 24.3 184.9 45.2 230.1 205.8 - 4.4 201. 4 1 a.m. to 7 a.m . Total 30, 316 .795 24.1 168.9 40.4 209.3 185.2 - 3.0 182.2 119, 712 97.8 724. 9 173.3 898.2 800.4 - .9 799. 5 Total, 3 days... Experiment No.28. 354, 472 296.7 2, 208. 5 528.3 2, 736. 8 2, 440. 1 - 4.9 2, 435. 2 Feb. 20-21 7 a. m. to 1 p. m . 27,985 .836 23.4 204.6 43.7 248.3 224.9 + 7.0 231. 9 1 p. m. to 7 p. m . 27,208 ..845 23.0 203.6 37.6 241.2 218,2 + .2 218.4 7 p.m. to 1 a.m . 28, 762 .850 24.4 191.4 43.1 234.5 210.1 - 5.3 204.8 1 a. m. to 7 a. m . Total 28, 762 .836 24.0 170.2 39.3 209.5 185.5 - 4.0 181. 5 112, 717 94.8 769. 8 163.7 933. 5 838.7 - 2.1 836.6 7 a.m. to 1 p. m . 21-22 26, 430 .848 22.4 186.4 38.9 225. 3 202. 9 + 9.5 212.4 1 p. m. to 7 p. m . 27, 208 .806 21.9 201.4 38.2 239.6 217. 7 -10.1 207.6 7 p.m. to 1 a.m . 28, 762 .867 24.9 187.9 43.9 231.8 206.9 + 5.9 212.8 1 a. m. to 7 a. m . Total 26, 430 .796 21.0 166.7 35.9 202.6 181.6 - .6 181.0 108, 830 90.2 23.8 742. 4 156.9 899.3 809.1 + 4.7 813.8 7 a.m. to 1 p. m . 22-23 2G, 430 .899 185. 2 39.2 224. 4 200. 6 + 3.3 203.9 1 p. m. to 7 p. m . 27,208 .890 24.2 194.8 37.4 232.2 208. + .5 208. 5 7 p. m. to 1 a. m . 28, 762 .917 26.4 183.7 43.9 227.6 201.2 - 2.8 198.4 1 a.m. to 7 a.m . Total 28, 762 .816 23.5 167.0 39.4 206.4 182.9 - 3.1 179.8 111, 162 97.9 730.7 159.9 890.6 792. 7 - 2.1 790.6 Total. 3 days... 332, 709 282.9 2, 242. 9 480.5 2, 723. 4 2, 440. 5 - .5 2, 441. 90 The heat carried away by the water current and the latent heat of vaporization of water in experiments Xos. 26 and 28 are shown in Table 106. Table 106. — Sam/nary of calorimetric measurements — Metabolism experiments Xos. 26 and §8. (a) (b) (c) (d) (e) Water (/) iff) Date. Period. Heat measured in terms of Co . Change of tem- perature of calo- rimeter. Capacity correc- tion of calo- rimeter, 6x60. Correc- tion due to tem- perature of food and dishes. vapo- rized equals total amount exhaled less amount con- densed Heat used in vapori- zation of water, ex 0.592. Total heat deter- mined, u-c- d+f. in chamber. 1900. Experiment Xo. 26. Calories. Degree. Calories. Calories. Grants. Calories. Calories. Feb. 14-15 7 a.m. to 1 p. m .. 525. 5 -0.2 -1.2 - 2.1 222. 131.4 653.6 1 p.m. to 7 p. m .. 453. 2 - .1 - .6 - 3.9 215.3 127.5 576. 2 7 p.m. to 1 a. m .. 350.9 - .1 - .6 + 7.7 205. 6 121.7 479.7 1 a.m. to 7 a.m... Total 254. 5 + .5 +3.0 186.2 110.2 367.7 1,584.] + .1 + .6 + 1.7 829. 1 490.8 2. 077. 2 15-16 16-17 Feb 20-21 21-22 22-23 1 p. m. to 7 p. m . . 7 p. m. to 1 a. m 1 a.m. to 7 a.m. Total. 7 a.m. to 1 p. m .. 1 p.m. to 7 p. m .. 7 p.m. to 1 a. m .. 1 a.m. to 7 a.m... Total Total, 3days. Experiment Xo. -2S. 7 a.m. to 1 p. m .. 1 p. m. to 7 p. m 7 p.m. to 1 a. m .. 1 a.m. to 7 a.m... Total 7 a.m. to 1 p. rn .. 1 p.m. to 7 p. m .. 7 p.m. to 1 a. m .. 1 a.m. to 7 a.m... Total 7 a. m. to 1 p. m . . 1 p.m. to 7 p. m .. 7 p.m. to 1 a. m .. 1 a.m. to 7 a. m .. Total Total, 3 days. 522.8 + • - 201. 9 119.5 642. 5 465. 5 .1 - .6 - 3.8 211.2 125.0 586.1 388.2 + .1 -f .6 + 6.1 204. 9 121. 3 516.2 241. 8 + .3 + 1.8 C 188.6 111.7 355.3 1.618.3 + .3 + 1.8 + 2.5 806.6 477.5 2, 100. 1 510. 7 - 3.1 206.1 122. 635. 8 468.9 — . 7 209. 8 124. 2 592, 4 371.4 + .1 + .6 + 9.6 201.4 119.2 500.8 241.3 182. 2 107.9 349.2 1,592.3 + .1 +0.6 +12. C 799.5 473.3 2. 078. 2 4.7V»4.7 +3.0 + 16.2 2, 435. 2 1.441.6 6. 255. 5 526.0 - .3 231.9 137.3 663.0 472. 5 - .8 218. 4 129. 3 601.0 361.9 - .3 -1.8 + 3.9 204. 8 121.2 485.2 239. 1 + .3 +1.8 181.5 107.4 348.3 1,599.5 + 2.8 836.6 495. 2 2. 097. 5 501.0 + 2.1 212. 4 125. 7 628. 8 141.5 - 3.6 207.6 122.9 560. 8 393.6 + .2 + 1.2 + 4.1 212. 8 126. 524.9 253. 6 181.0 107. 2 360.8 1.589.7 + .2 + 1.2 + 2.6 M3.8 481.8 2.075.3 507.7 + 1.0 203.9 120.7 629.4 456.2 - 7.0 208.5 123. 4 572. 6 378.6 + .1 +0.6 + 8.2 198.4 117.5 504.9 251. 7 179.8 106.4 35a i 1.594.2 + .1 +0.6 + 2.2 790.6 468.0 2, 065. 4. 783. 4 4-1.8 + 7.6 2,441.0 1,445.0 6.237.8 91 Balance of vnconu and outgo of matter and energy, — Tables 107-110 summarize the income and outgo of nitrogen, carbon, hydrogen, and energy in this series of experiments. Table 107. — Income and outgo of nitrogen and carbon — Metabolism experiments Nos. 96 and 28. Nitrogen. Carbon. Date. (a) In food. (6) In feces. (c) In urine. {el) Gam or loss (-), a - (& + C). (c) In food. (/) In feces. (0) In urine. (h) In respir- atory prod- ucts. (*) Gain ( + ) or loss (-), e-(f+ 9+h). 1900. Experiment Xo. 26. Grams. Grams. Grams. Grams. Grams. Grams. Grams. Grams. Grams. Feb. 14-15. 7 a. in. to 7 a.m.. 15.9 1.1 16.6 -1.8 233. 2 9.4 11.9 194.9 +17.0 15-16,7 a.m. to 7 a.m.. 15.9 1.1 15.1 -0.3 233. 2 9.5 10.8 198.4 +14.5 16-17,7 a.m. to 7 a.m.. 15.9 1.1 14.4 +0.4 233.2 9.4 10.4 194. 9 +18.5 Total, 3 days 47.7 3.3 46.1 -1.7 699.6 28.3 33.1 588. 2 +50.0 Average. 1 day 15.9 1.1 15.4 -0.6 233. 2 9.4 11.0 196.1 +16.7 Experiment Xo. 28. Feb. 20-21, 7 a. m. to 7 a. m. . 15.8 1.2 15.9 -1.3 245.8 10.0 11.4 211.0 +13.4 21-22.7 a.m. to 7 a.m.. 15.7 1.3 15. 2 -0.8 245. 8 10.0 10.9 212.8 +12.1 22-23, 7 a. m. to 7 a.m.. 15.8 1.2 14.7 -0.1 245. 8 10.0 10.5 208. 3 +17.0 Total. 3 days 47.3 3.7 45.8 -2.2 737.4 30.0 32.8 632.1 +42. 5 Average, 1 day 15.8 1.2 15. 3 -0.7 245.8 10.0 10.9 210. 7 +14.2 Table 108. — Income and outgo of water and hydrogen — Metabolism expi riments Xos. 26 and 28. Water. Date, (a) In food. (6) In drink. (c) In feces. (d) In urine. (e) In respir- ator y products. Apparent loss, a+b -(c+d+ e). 1900. Experiment Xo. 26. Feb. 14-15, 7 a.m. to 7 a.m Grams. 1,046.3 1,046.3 1,046.3 Grams. 800 800 800 Grams. 57.0 57.0 57.0 Grams. 1,157.3 1, 472. 4 1,289.0 Grams. 829. 1 806.6 799.5 Grams. -197.1 15-16, 7 a. m. to 7 a. m -489. 7 16-17,7 a.m. to 7 a.m —299. 2 Total. 3 davs 3,138.9 1,046.3 2.400 800 171.0 57.0 3,918.7 1,306.2 2, 435. 2 811.7 -986.0 -328. 6 Experiment X<>. 98. Feb 20-21,7 a.m. to 7 a.m 21-22, 7 a.m. to 7 a. m 1,040.0 1,040.0 1,040.0 800 800 800 51.7 51.8 51.7 1,113.2 1,238.0 1,150.3 836.6 813.8 790.6 -161.5 —263.6 22-23, 7 a.m. to 7 a.m -152.6 Total, 3 days Average. 1 dav 3.120.0 1,040.0 2,400 800 155.2 51.7 3,501.5 1,167.2 2,441.0 813.7 -577. 7 -192.6 92 Table 108. — Incorm and outgo of vjater and hydrogen — Metabolism experiments Nos. 26 a nd 28 — Cont inued . Hydrogen. Date. (9) (h) In food. In feces. (i) (I) (m) Apparent Loss from In urine, gain, {g— water, / [h+i). h-9. (n) ■ Total gain( + ) or loss (-).M-wi. 1900. Experiment Xo. 26. Grams. Grams. 33.9 ; 1.1 33.8 1.2 33.9 1.1 Grams. Grams. 2.9 +29.9 2.6 , +30.0 2.5 +30.3 Grams. - 21.9 - -54.4 - 33.2 Gravis. + 8.0 -24. 4 16-17,7 a. m. to 7 a.m - 2.9 Total, 3 davs 101.6 3.4 33.9 1-1 8.0 j +90.2 2.7 1 +30.1 -109. 5 - 36.5 —19.3 Average. 1 dav -6.4 Experiment No. 28. Feb. 20-21, 7 a. m. to 7 a. m 21-22,7 a.m. to 7 a.m 22-23,7 a.m. to 7 a.m 35.8 35. 8 35.8 1.3 1.4 1.3 2.8 +31.7 2.6 1 +31.8 2.5 +32.0 - 17.9 - 29.3 - 17.0 +13.8 - 2.5 -15.0 Total. 3 davs 107.4 35.8 4.0 1.3 7.9 +95.5 2.7 ! +31.8 - 64.2 - 21.4 -r31.3 -10.4 Table 109. — Gain or loss of protein {NX 6.25), fat, and water — Metabolism experiments Nos. 26 and 28. Date, (a) Nitrogen gained < - | or lost (-). m Protein gained ( + )or lost (-), ax6.25. (c) Total carbon gained ( + )or lost (-). (d) Carbon in pro- tein gained ( + ) or lost (-), 6x0.53. Carbon in fat, etc.. gained ( + )or lost (-), c-d. (/) Fat gained (+) or lost (— ), e-^0.761. 1900. Experiment No. 26. Feb. 14-15, 7 a. m. to 7 a. m Grams. -1.8 - .3 + .4 Grams. -11.2 - 1.9 + 2.5 Grams. -17.0 +14.5 +18.5 Grams. -5.9 -1.0 +1.3 Grams. +22.9 +15. 5 +17.2 Grams. +30.1 -20. 4 +22. 6 Total , 3 davs -1.7 - .6 -10.6 - 3.5 +50.0 +16.7 -5.6 -1.8 -55.6 4-18. 5 +73.1 +24.4 Experiment No. 28. Feb. 20-21, 7 a. m. to 7 a. m -1.3 - .8 - .1 - 8.1 - 5.0 - .6 -13.4 + 12. 1 +17.0 -4.3 -2.7 - .3 + 17.7 -14.8 -17.3 -23.3 21-22,7 a.m. to 7 a.m -19.4 22-23,7 a.m. to 7 a.m -22. 7 Total. 3 da vs -2.2 — . 7 -13.7 - 4.5 +42.5 + 14.2 -7.3 -2.4 +49.8 +16.6 -65. 4 -21. 8 93 Table 109. — Gain m loss of protein (NX6.g5) 1 fat, and water — Metabolism experiments Xos. 96 and 98 — Continued. Date is) Total hydrogen gained ( + ) or lost (A) Hydrogen Hydrogen in" protein in fat gained (+) gained - orlost( — ). orlost(-), 6x0.07. /X0.118. (*) Hydrogen in water. "Water etc.. trained gained (, + ) ( + ) or lost or lost ( — ), (-), *x9. g-{h- 1900. Experinu nt No. 96. Feb. 14-15, 7 a. m. to 7 a. m Grams - 8.0 -24. 4 - 2.9 Gram. - .1 Grams. -3.6 +2. 4 j_2 7 Grams. + 5.2 -26.7 — 5. S Grams. - 46. S -240.3 - 52.2 Total . 3 da vs -19.3 - 6.4 — • " — 2. it -27.3 - 9.1 -245.7 - SI. 9 Experiment No. - 7 >. Feb. 20-21. 7 a.m. to 7 a.m +13. 8 + 2.5 +15. - .6 - .3 +2.7 -2.3 +2.7 +11.7 + .5 +12. 3 +105. 3 - 4.5 22-23. 7a.rn.to7a.rn +110.7 Total . 3 days -31.3 +10.4 - .9 - .3 -2. 5 +24.5 + S.2 -220. 5 + 73.5 Table 110. — Income and outgo of energy — Metabolism experiments No*. 96 and 98. (a) : (o) (d) | (e) if) iff) (A) (0 Date. Heat of com- bus- tion of food eaten. Heat of com- bus- tion of feces. Esti- | Esti- mated mated heat of heat of Heat ' com- com- ofcom- bustion bos- bos- of pro- tion of tion of tein fat urine, gained gained Esti- mated energy of ma- terial oxi- dized in the bodv. Heat deter- mined. Heat deter- mined greater (+ \ or less(-) than esti- Heat deter- mined greater ( + )or lea -) than esti- V-t- I ui lost (-). lost a-(b+ c+el -e). mated. o-f- mated, 1900. Experiment No. Calo- Calo- Calo- Calo- Calo- Calo- Calo- Calo- ries. ries. ries. ries. ries. rus. ries. ries. Per. et. Feb. 14-15. 7 a. m. to 7 a. m. . . 2.490 106 125 -64 +287 2.036 2. 077 + 41 +2.0 15-16. 7 a. m. to 7 a. m. . . 2.490 106 125 -11 +195 2. 075 2.100 - 25 + 1.2 16-17. 7 a. m. to 7 a. m. . . 2. 490 106 135 -14 +216 2.019 2. 07^ + 59 +2.9 Total. 3 days 7.470 318 -61 +698 6. 130 6. 255 +125 Average. 1 day 2,490 106 128 -•20 -233 2. 043 2,085 + 42 -2. Experinu nt No. - • Feb. 20-21. 7 a. m. to 7a.m... 2,489 112 119 -47 -222 - S 2.097 + 14 + .7 21-22. 7 a. m. to 7 a. m. . . 112 133 -29 +185 2,088 2,075 - 13 - .6 22-23.7 a.m. to 7 a.m... 2, 189 112 132 - 3 -217 2. 031 2. 065 - 34 +1.7 Total. 3davs 7,467 336 384 -79 -624 6.202 6. 237 + 35 Average,! day 2, 489 112 128 -26 +208 2.067 2. 079 - 12 + .6 94 METABOLISM EXPERIMENTS NOS. 29 AND 31. Subject. — J. F. S., the same person as in experiments Nos. 25, 26, and 28, His weight, with underclothing', was about 64.5 kilograms (142 pounds). Occupation during experiment. — Worked 8 hours a day upon a sta- tionary bicycle arranged as an ergometer, as described on page 20. The voltage of the electric current generated was measured, and the average number of pedal revolutions per minute observed. The cur- rent generated was passed through resistance within the chamber and thus transformed into heat, which was measured with the heat given off by the subject. The actual amount of work done each day was found by determining the watts required to drive the bicycle ergometer at the rate maintained by the subject during the experiment. Duration. — Experiments Xos. 29 and 31 were the first and third of a series of 3, each of 3 days* duration. The intervening experiment is not reported here. The usual preliminary period continued •! days, beginning with breakfast March 12, 1900. On the evening of the fourth day. March 15, the subject entered the calorimeter. Experi- ment No. 29 began at 7 a. m. March 16, and ended at 7 a. m. March 19. Experiment No. 31 began at 7 a. m. March 22, and ended at 7 a. m. March 25. Diet. — The aim of this series of experiments was to study the rela- tive replacing power of isodynamic quantities of different materials when the subject was at active exercise. There was. as usual, a basal ration supplemented by different materials. This basal ration was the same in both experiments, with the exception of slight differences due to variations in the composition of the milk consumed. It furnished approximately 100 grams of protein and 2,980 calories of energy per day. To this ration was added 128 grams of cane sugar per day. fur- nishing 507 calories of energy in experiment No. 29. and 63.5 grams of butter per day, furnishing 1 gram of protein and 511 calories of energy, in experiment No. 31. The kinds and quantities of food served at each meal and the quantities of drink at different periods of the day were as follows: Table 111. — Diet in metabolism experiments Xos. 29 and 31. FOOD— BASAL RATION. Food materials. Breakfast. Dinner. Supper. Total. Beef .. Grams. Grams. 58 23 300 150 25 Gnms. Grams. 58 Butter 12 300 75 25 37.5 12.5 12 300 75 25 37.5 12.5 47 Milk, whole 900 Bread 300 75 75 25 95 FOOD— SUPPLEMENTAL RATION. Experiment No. ?9, March 16-18. — One hundred and twenty-eight grams of cane sngar daily in the form of loaf sugar, taken with and between meals. This amount also supplemented the basal ration during the preliminary experiment March 12-15. Experiment No. SI. March S8-ZU.— The additional energy during this experiment was furnished by 63.5 grams butter. DRINK. Time. Water. Time. Water. 1 Grams. Breakfast 150 10.15 a. m 200 Dinner 200 4.00 p. m 200 Supper 150 Gra7ns. 9.00 p. m 200 10.20 p.m 150 Total for day 1. 250 Daily routine. — The general plan of the series of experiments is indicated in the following schedule: Table 112. — Daily programme — Metabolism experiments Nos. 29 and 31. 6.50 a. in Take pulse and temperature •4.00 p.m Stop work, drink 200 grams water. 7.00 a. m Pass urine, weigh self, collect 4.15 p.m Begin work. drip, and weigh absorbers. 6.15 p.m Stop work, change underclothing. 7.30 a. m Breakfast.drink 150 grams water. 6.20 p.m Supper, drink 150 grams water. 8.15 a.m Begin work. 6.50 p. m Take pulse and temperature. 9.15 a.m Stop work. drink 200 grams water. 7.00 p.m Pass urine, weigh self, collect 10.30 a.m Begin work. drip, and weigh absorbers. 12.30 p. m Stop work. 9.00 p. m Drink 200 grams water. 12.50 p.m Take pulse and temperature. 10.00 p. m Take pulse and temperature. 1.00 p. m Pass urine, collect drip, and 10.10 p.m Arrange bed. weigh absorbers. 10.20 p.m Drink 150 grains water. 1.25 p.m Dinner, drink 200 grams water. 10.30 p.m Retire. 2.00 p.m Begin work. 1.00 a.m Pass urine. Table 113 gives a condensed summary of the more important statis- tics in the diary kept by the subject. 96 Table 113.- -Summary of the diary — Metabolism experiments Nos. 29 aid 31. Time. Weight of subject in under- clothes. Pulse rate per minute. 106 112 111 86 Temper- ature. Time. Weight of subject in under- clothes. Pulse rate per minute. Temper- ature. 1900. Preliminary obser- vation. Mar. 15, 9.40 a. m Kgs. °F~. 1900. Experiment No. 29— Continued. Mar 18, 10.20 p. m... Kgs. 66 R 10.25 p. m... 96 4 12m Experiment No. 31. Mar. 22, 6.55 a. m.... 7 a. m 64.09 65 12 55 p. m... 97.8 7 p. m 64.51 97.6 92 87 71 90 85 87 90 79 101 108 102 88 83 82 66 92 96 94 94 74 93 98 93 94 77 76 99.0 98.4 97.6 9 a. m 93 87 90 87 67 99 93 97 93 71 10 a. m 63.85 11 a. m Experiment No 29. 12 m Mar. 16, 7 a. m 1 p. m 97.8 9 a. m 3 p. m 10 a. m 11 a. m 12 m 6 p. m 1 p. m 98.5 6.55 p. m 7 p. m 8 p. m 64.55 76 70 67 68 97.5 9 p. m 97.0 10.12 p. m... Mar 23 6 55 a. m 96.5 7 p. m 64.78 97.6 98.2 97.4 7 a. m 64.24 Mar. 17, 7 a. m 64.76 100 92 89 89 97 94 89 90 74 75 68 66 65 89 95 86 88 68 10 a. m 10 a. m 12 m 12 n 98.4 6 p. m 7p.m 8 p. m 64.68 97.6 97.5 7 p. m 8 08 p m . 65.12 97.9 10.10 p. m... 97.6 97.4 Mar. 24, 6.55 a. m.... 64.38 75 69 10 a. m 96.9 97.3 Mar. 18, 7 a. m 64.76 65 88 93 91 92 69 91 95 95 93 79 74 12 m 12.55 p. m... 97.8 3 p. m 98 98 91 90 76 73 71 12 m 98.0 6 p. m 7 p. m 8 p. m 64.90 97.4 97.3 7 p. m 64.96 97.8 96.9 10.05 p. m... 66 97.4 97.2 10.10 p. m... 96.7 9.15 p. m ... 77 Mar. 25, 6.55 a. m 64.49 68 97.9 97 Amount of work done. — The total number of miles registered by the cyclometer on the different days of this series of experiments and the heat equivalent of the work done each day are shown in Table 114. It is altogether improbable that the amount of work done could have been as large as would be required to propel a bicycle, under ordinary conditions, the distance indicated by the cyclometer. Table 114. — Record >>/ work dont — Metabolism experiments Nos. 29 and 31. Date and time. Cyclometer reading. Number Actual of miles. duration of work. (b) Rate. (c) Heat equivalent, 0.2378 1900. Experiment y 89. 8.15 a.m Mar. 16 16. 10.15 a.m... 12.30 p.m... 16. 4.00p.m... 16. 6.15p.m... Total Mar. 17. 10.15 a.m. 17. 12.30 p. m . 17. 4.00 p.m. 15 p.m. Total Mar. In 10.15 a.m. 1>. 12 In 4.00p.m. 15 p.m. Total Total. 3 days Mar. 2 Mar. 23. 10.15 a.m.. 23. 12.30 p.m.. 23. 4.00 p.m.. 1'p.m.. Mar. 24. 10.15 a.m. 21. 12.30 p.m. 24. 4.00p.m. 24. 6.15 p.m. Total Total. 3 days. 666.0 57 708. 4 ■_ 3 751.1 - 1 - m 906.9 930. 4 nds. Watts. Calories. 21.5 7.200 42.0 72 20.9 7.2lX> 39.7 68 21.4 7.200 09.5 21.3 37.7 85.1 28,560 270 35. 5 6 7. 200 37. 2 4. 9S0 42. 4 24.8 7.200 39.0 - - - 340 239 23.9 7,200 36.5 62 23.2 7,200 35. 7 61 21.9 7.200 37.4 04 23.5 J - 40.0 69 •- ' 28,800 2-56 _ L4 B3.700 765 772. 3 21.2 35.5 59 795. 9 23. 6 7. 200 37.0 63 813. 1 17.2 4. 9S0 42.4 50 Experiment No. 22. 10.15 a.m 1.194.4 21.2 7,200 37.4 64 22. 12.30 p. m 1,218.0 23. 6 7.200 22. 4.00 p. m 1.240.9 22. 9 7.200 39.0 22. 6.15/p. m 1,262.9 22. 7. 200 37.0 63 Total ..." - ■ " 28,800 ... 260 L.2S 3 1,306.8 1.329.9 1. 351. 4 _ - 17.1 23.1 21. 5 7. 200 7.200 7. 200 7.200 >8 - 96.0 27. -40 37.0 37.4 34.4 1,375 • 24.4 7. 200 37.0 63 1.400.7 24.9 7. 200 3^.7 61 1.423.7 23.0 6. 240 3-5.7 53 1.447.4 23. 7 7.200 34.9 60 - 13007— No. 109—02- 98 Detailed data of income and outgo. — The quantities of nutrients in the basal and supplemental rations during 1 this series of experi- ments are shown in Table LI 5. The outgo of matter and energy in the feces during the successive experiments is shown in Table 11(3. Tables 117 and 118 show the amount and composition of the urine in experiments Nos. 29 and 31. Table 115. — Weight, composition, and heal of combustion of foods — Metabolism experi- ments Nos. 29 and 31. Lab- ora- tory No. Food materials. Weight per day. Water. Pro- tein. Pat. Carbo- hy- drates. Nitro- gen. Car- bon. Hydro- gen. Heat of com- bus- tion. 3186 Beef Grams. 58 47 300 75 75 25 Grams. 35. 4.3 109.5 3.1 3.1 Grams. 20.7 .6 28.2 4.7 9.0 drams. 1.7 40.6 6.0 6.2 1.1 Grams. 152. 4 59. 9 60.4 25. Grams. 3.32 .09 4.50 . 75 1.44 Grams. 12. 12 30.60 ST. 42 33. 24 32. 04 10. 52 Grams. 1.73 * 4.91 12.90 4.96 4.72 1.62 Calo- ries. 135 3187 3192 Butter 378 879 3181 333 3193 Parched cereal Sugar Basal ration, exclu- sive of milk EXPERIMENT NO. 29. Milk, whole Total basal ration.. Loaf sugar (supple- mental ration) Total ration, 1 day. EXPERIMENT NO. 31. Basal ration, exclu- sive of milk Milk, whole Total basal ration.. Butter (supplement- 315 99 3189 580 900 155. 760.5 63.2 36.9 55.6 50.4 297. 7 45.0 10.10 5.94 205. 94 73.80 30.84 11.34 2, 139 s41 1,480 128 915.5 100.1 106.0 342. 7 128.0 16.04 279. 74 53.89 42. is 8 29 2, 980 .",1)7 1,608 915.5 100.1 106.0 470.7 16.04 333.63 50.47 3.4S7 3191 580 900 155.0 760.5 63.2 36.9 56. 6 50.4 297. 7 45.0 10.10 5.85 205. 94 74.25 30. 84 2, 139 11.34 845 3187 1,480 63.5 915.5 5.8 100.1 .8 106.0 54.8 342.7 15.95 .13 280. 19 41.34 42.18 6.63 2, 984 ' SI 1 Total ration, 1 day. 1,543.5 921.1 100.9 160.8 MM 16.08 321. 53 4S.S1 3.495 Table 116. — Weight, composition, and heat of combustion of feces — Metabolism experi- ments Nos. 29 and 31. Lab- ora- tory No. Weight of feces. Water. Pro- .,. ^v; 1 " 1 - Xitro- Car- bon. Hydro- gen. Heat of combus- tion. Experiment No. 29. 3195 Total, 3 days Average, 1 day . . . Grams. 177.0 59.0 Grams. 123.7 41.2 Grams, annus. Grams. Grams. Grams. Grams. 15.9 9.D 18.2 2.55 25.01 3.6 5. 3 | 3. 6. 1 . 85 8. 34 1 . 2 Calories. 279 93 Experiment No. SI. 3197 Total. 3 days Average, 1 day... 160.1 108.1 15.2 8.2 53.4 36.0 5.1 2.7 18.1 2.43 6.0 .81 24. 32 3. 4 8.11 1.1 272 91 99 Table 117. — Amount, specific gravity, and nitrogen of urine, by 6-hour periods — Metab- olism experiments Xos. 29 and 31. Date. Period. Amount of urine. Specific gravity. Nitrogen content. 1900. Mar 16 17 Experiment Xo. 29. Grams. 169. 5 215. 7 171.2 138.5 1.032 1.032 1. 035 1.034 Per cent. 2.14 1.99 2.39 2.33 Grams. 3.63 1 p. m. to 7 p. m 7 p.m. to 1 a. m 4.29 4.09 3. 23 Total 694.9 694.9 15. 24 1.034 2.21 15.36 7 a. m. to 1 p. m 1 p. m. to 7 p. m 7 p. m. to 1 "A. m 17-18 189.2 252. 6 183.6 151.8 1.031 1.031 1.033 1.032 2.05 1.83 2.26 2.28 3.88 4. 62 4.15 3.46 Total Total by composite 7 a.m. to 1 p. m 1 p. m. to 7 p. m 7 p. m. to 1 a. m 1 a. m. to 7 a. m Total Total by composite 777.2 777. 2 16.11 1.031 2.06 16.01 18-19 227. 5 342.0 182. 1 139.2 1.029 1.029 1.032 1. 032 1.75 1.44 2.15 2.27 3.98 4.92 3.91 3.16 890.8 890.8 15. 97 1.030 1.80 16. 03 Total 3 days, by periods Total by composite Experiment No. 31. 7 a. m. to 1 p. m 1 p. m. to 7 p. m 2,362.9 2, 362. 9 47. 32 47. 4U Mar. 22-23 252. 3 247.3 172.3 140.4 1.027 1.030 1.033 1.031 1.62 1.83 2.39 2.36 4.09 4. 53 4.12 1 a. m. to 7 a. m Total Total by composite 7 a. m. to 1 p. m 1 p. m. to 7 p. m 7 p. m. to 1 a. m 1 a. m. to 7 a. m . Total Total by composite 7 a. m. to 1 p. m • 1 p. m. to 7 p. m 7 p. m. to 1 a. m la.rn.to7a.rn Total Total by composite Total 3 days, by periods Total by composite 3.31 812.3 812.3 16. 05 1.030 1.99 16. 16 23-24 213. 2 267. 173.3 137.0 1.029 1.028 1.031 1.032 1.84 1.62 2.22 2. 29 3.92 4.33 3.85 3.14 790.5 790.5 15.24 1.030 1.91 15. 10 21-25 240. 3 293. 5 188.0 158.2 1.028 1.028 1.033 1.032 1. 55 1.40 2.04 2.12 3.72 4.11 3.84 3.35 880.0 880.0 15. 02 1.030 1.72 15.14 2, 482. 8 2,482.8 46. 31 16. 40 8,006.2 1.78 142 50 3 Including 3 days of an experiment not here reported. 100 Table 118. — Daily elimination of carbon, hydrogen, water, and energy in urine — Metab- olism experiments Nos. 29 and 31. Date. Period. Amount of urine. Carbon. Water. Heat of com- bustion. Per gram. Total. 1900. Mar. 16-17 17-18 18-19 Experiment No. 29. 7 a.m. to 7 a. m 7 a.m. to 7 a.m 7 a. m. to 7 a. m Total Grams. 694.9 777.2 890.8 P.ct. Grams. 10.78 11.39 11.29 P.ct. Grams. 2.86 3.03 3.00 P.ct. Grams. 641.0 720.3 834.3 Calorie. 0.193 .173 .150 Calories. 134 134 134 2, 362. 9 33.46 8.89 2, 195. 6 402 Experiment No. 31. 7 a.m. to 7 a.m 7 a.m. to 7 a.m 7 a.m. to 7 a.m Total 22-23 23-24 24-25 812.3 790.5 880.0 11.35 10. 78 10.62 3.01 2.86 2.82 755. 6 736.6 826.9 .162 .163 .145 132 129 128 2, 482. 8 32.75 8.69 2, 319. 1 i 389 Total, 9 days «. 8, 006. 2 1.28 102. 49 0.34 27.22 93.6 7,493.8 1 1,211 "Including 3 days of an experiment not here reported. The quantities of carbon dioxid and water in the ventilating air cur- rent are given in detail for experiments Nos. 29 and 31, in Tables 119- 121, which follow. Table 122 shows the amount of heat given off dur- ing the successive 6-hour periods of the two experiments. 101 Table 119. — Comparison of residual amounts of carbon dioxid and water in the chamber at the beginning and end of each period, and the corresponding gain or loss — Metabolism i xperimenls No8. 29 and 31. End of period. Carbon dioxid. Water. Date. Total amount in chamber. Gain ( + ) or loss ( — ) over preced- ing period. Total amount of vapor remain- ing in chamber. Gain ( + ) or loss (— ) over preced- ing period. Change in weight of ab- sorbers, gain( + ) or loss (-). Drip from absorb- ers. a Total amount gained ( + )or lost(-) during the period. 1900. Mar 16 Experiment No. 29. Grams. 25.5 75.1 79.0 30.4 25.7 Grams. Grams. 46.7 53.8 53.0 50.2 47.1 Grams. Grams. Grams. Grams. 16 lp.m +49.6 + 3.9 -48.6 - 4.7 + 7.1 - .8 - 2.8 -3.1 + 98 +194 - 69 - 69 130.0 236.0 22.7 12.0 +235. 1 16 7 p. m +429.2 17 1 a.m — 49.1 17 - 60.1 Total + .2 + .4 + 154 400.7 — 555. 1 1 p. m 17 85. 3 77.1 26.4 23.8 + 59.6 - 8.2 -50.7 - 2.6 53.6 52. 4 48.3 44.7 + 6.5 - 1.2 - 4.1 - 3.6 + 18 + 17 - 93 - 94 180.0 281. 9 31.9 19.2 +204. 5 17 7 p. m +297. 7 18 1 a. m - 65.2 18 - 78.4 Total - 1.9 - 2.4 -152 513.0 +358.6 18 83.2 81.9 28.5 26.8 +59.4 - 1.8 -53.4 - 1.7 51.7 54.5 50.7 48.1 + 7.0 + 2.8 - 3.8 - 2.6 + 169 + 83 -110 -110 120. 370.2 54.2 40.0 +296. 18 7 p. m +456. 19 1 a. m — 59.6 19 — 72.6 Total + 3.0 + 3.4 + 32 584.4 +619.8 Experiment No. SI. Mar 22 26.4 78.9 75. 2 26.2 25.7 22 22 23 23 1 p. m 7 p. m 1 a.m 7 a. m Total 1 p. m 7 p. m 1 a.m 7 a.m +52.5 - 3.7 -49.0 - .5 55.3 52.4 48.1 45.2 +10.1 - 2.9 - 4.3 - 2.9 +198 - 17 - 88 - 88 150.0 369. 2 29.6 20.0 +358.1 +349. 3 - 62.7 - 70.9 - .7 + 5 568.8 +573. 8 23 23 24 24 75.6 76.8 25.1 24.7 +49.9 + 1.2 —51.7 - .4 54.6 52.8 50.2 45. 2 + 9.4 - 1.8 - 2.6 - 5.0 +180 - 15 - 83 - 83 120.0 ■ 357. 7 26.4 18.0 +309. 4 +340.9 - 59.2 - 70.0 Total - 1 522. 1 +521. 1 24 78.1 70.3 25.5 27.6 +53.4 '- 7.8 -44.8 + 2.1 54.9 52. 4 48.3 47.9 + 9.7 - 2.5 - 4.1 - .4 +173 - 5 - 76 - 76 135. 364.4 28.9 19.0 +317.7 24 25 7 p. m 1 a. m +356. 9 - 51.2 25 — 57.4 Total + 2.9 + 2.7 + 16 547. 3 +566. »The drip was collected and weighed but once a day. The volume was roughly observed at 1 p.m., 7 p.m., and 7 a.m., and this volume taken as a rough indication of the actual weight of drip for the different periods. The small amount of drip observed at 7 a. in. was divided equally between the two night periods. The figures in this column also include the perspiration in clothes, which amounted to 21, 23.8, 20.4, 18.3, 15.3, and 18.8 grams on the successive days of the series. The amount for each day has been divided equally between the periods ending at 1 p. m. and 7 p. m. 102 Table 120. — Record of carbon dioxid in ventilating air current — Metabolism experiments Nos. 29 and SI. Period. (a) Venti- lation (num- ber of liters of airl. Carbon dioxid . m.. 28, 762 . 594 17.1 147.3 130.2 - 2.6 127. 6 34. 8 110, 385 65.5 1, 252. 7 1,187.2 - 1.9 1,185.3 323. 3 18-19 27, 208 .564 15.4 415. 1 399.7 +59.4 459.1 125.2 1 p.m. to 7 p.m.. 27,208 .514 14.0 492. 8 478.8 - 1.3 477.5 130.2 7 p.m. to 1 a.m.. 27, 985 .601 16.8 262. 1 245. 3 -53.4 191.9 52. 3 1 a.m. to 7 a. m.. Total Total, 3 days Experiment No. 31. 26, 430 .576 15.2 1 15. 2 130.0 - 1.7 128.3 35.0 108,831 61.4 1,315.2 1,253.8 + 3.0 1,256.8 342. 7 329, 602 192.1 3,874.6 3, 682. 5 + 1.8 3, 683. 8 1,004.7 Mar. 22-23 7 a. m. to 1 p. m . . 25, 652 .578 14.8 387. 5 372.7 +52.5 425. 2 116.0 1 p.m. to 7 p.m.. 26,430 .600 15.8 447.7 431.9 3.7 128. 2 116.8 7 p.m. to 1 a.m.. 27, 985 .580 16.2 232.0 215. 8 -49.0 166.8 45. 5 1 a.m. to 7 a.m.. Total 7 a. m. to 1 p. in.. 26,430 .563 14.9 143.0 128.1 — .5 127.6 34. 8 106, 497 61.7 1,210.2 1,148.5 ^ .7 1,147.8 313.1 23-24 25, 652 .578 14.8 381.7 366. 9 +49.9 416.8 113.7 1 p.m. to 7 p.m.. 25, 652 .589 15.1 443. 4 128.3 + 1.2 429. 5 117.1 7 p.m. to 1 a.m.. 27, 985 .567 15.9 243. 3 227. 4 -51.7 175. 7 17.9 1 a.m. to 7 a. ml. Total 7 a.m. to 1 p.m.. 28, 762 . 555 16.0 155.2 139.2 - .4 138.8 37.9 108, 051 ; 61.8 1,223.6 1,161.8 - 1.0 1,160.8 316. 6 24-25 25, 652 .568 14.6 378. 5 363.9 +53.4 417.3 113.8 1 p.m. to 7 p.m.. 27, 208 .581 15.8 455. 4 439.6 - 7.8 431.8 117.8 7 p.m. to 1 a.m.. 27, 985 .574 16.1 235. 218. 9 -44.8 174.1 47.5 1 a.m. to 7 a.m.. Total Total, 3 days 27, 985 .564 15.8 155. 6 139.8 + 2.1 141.9 38.7 108, 830 62.3 1,224.5 1,162.2 + 2.9 1,165.1 317.8 323, 378 185. 8 3, 658. 3 3,472.5 + 1.2 3. 473. 7 947.5 103 Table 121. — Record of wetter in ventilating air current — Metabolism eocperiments Xos. 29 and SI. Period. (a) = " E 00 O^ so "z. -■- > Water in in- coming air. Water in outgoing air. (9) (h) ~~ '-- s-S s as S3 - -~~ - o ■ - -- 14 lis! U) j it Date. (c) X e "3 o Amountcon- ^ densed in a< freezers. §8.5 < + 1 o m fa§ 1900. Experiment No. 89. Lit* rs. Mg. Grain*. Grams. Grams. Grams. Grams. Grams. Grams. Mai. 10-17 7a. m. to 1 p.m.. 27,208 0.850 23. 1 250. 5 46. 1 296.6 273.5 +235.1 508. 6 1p.m. to 7 p.m.. 27, 985 . 823 23.0 268. 44.7 312. 7 289.7 +429.2 718.9 7 p.m. to 1 a.m.. 27, 985 . 825 23.1 257. 5 45.1 302. 6 279.5 - 49.1 230. 4 1 a.m. to 7 a.m.. 27, 208 .756 20. 6 249. 5 40.8 290. 3 269.7 - 60.1 209. 6 Total 7a.m. to 1 p.m.. 110, 386 89. 8 1,025.5 176.7 1,202.2 1,112.4 1+555.1 1,667.5 17-18 25, 653 . 832 21.4 233.1 42.6 275.7 254.3 4-204.5 458.8 1p.m. to 7 p.m.. 27,208 . 849 23. 1 268. 8 43.6 312. 1 289. 3 -297. 7 587. 7p.m. to 1 a.m.. 28,762 .840 24.2 247.2 49.5 296.7 272.5 - 65.2 207. 3 1 a.m. to 7 a.m.. 28, 762 .780 22.4 243. 4 43.7 287.1 , 264.7 - 78.4 186. 3 Total 7 a.m. to 1 p.m.. 110,385 91.1 992. 5 179.4 1.171.9 1,080.8 +358.6 1,439.4 18-19 27, 208 .826 22. 5 243. 46.6 289. 6 267.1 +296.0 563. 1 1 p.m. to 7 p.m.. 27,208 .847 23. 282. 42.9 324.9 301.9 +456.0 757.9 7 p.m. to 1 a.m.. 27, 985 .875 24. 5 265. 17.8 312. 8 288.3 - 59.6 22^.7 1 a.m. to 7 a.m.. Total Total, 3 days Experiment No.Sl. 26,430 .861 22.8 243. 9 38.3 282. 2 259.4 - 72.6 186.8 108,831 92. 8 1,033.9 175.6 1,209.5 1,116.7 4-619.8 1,736.5 329,602 273. 7 3,051.9 531.7 3,583.6 3,309.9 4-1,533.5 4,843.4 Mar. '22-23 7 a.m. to 1 p.m.. 25, 652 . 828 21.2 247. 7 40.4 288.1 266.9 4-358.1 625. 1 p.m. to 7 p.m.. 26, 430 .857 22.6 259. 4 39.8 299.2 276.6 +349.3 625. 9 7 p.m. to 1 a.m.. 27,985 .816 22. s 246. 3 41.2 287.5 264.7 - 62.7 202. 1 a.m. to 7 a.m.. Total 7 a. m. to 1 p. in.. 26, 430 .779 20.6 241.0 35.0 276.0 255.4 l- 70.9 184. 5 106,497 87. 2 994. 4 156.4 1,150.8 1,063.6 1+573.8 1.637.4 23-24 25,652 . 865 22. 2 240. 5 40.8 281.3 259.1 +309.4 568. 5 1 p.m. to 7 p.m.. 25, 652 .881 22. 6 253. 3 37.8 291.1 268.5 +340.9 609. 4 7 p.m. to 1 a.m.. 27, 985 . 826 23.1 252. 43. 2 295. 2 272.1 - 59.2 212.9 1 a.m. to 7 a.m.. Total 7a.m. to 1 p.m.. 28,762 .780 22. 4 253. 2 38.5 291. 7 269.3 - 70.0 199. 3 108,051 ! 90.3 999. 160.3 1,159.3 1,069.0 +521.1 1 , 590. 1 24-25 25, 652 . 843 21.6 241.7 40.7 2S2. 4 260.8 +317.7 578. 5 1 p.m. to 7 p.m.. 27, 208 . 851 23. 2 269. 8 40.3 310. 1 286.9 +356.9 643. 8 7 p.m. to 1 a.m.. 27,985 .802 22. 4 247. 5 43.5 291.0 268.6 - 51.2 217.4 1 a.m. to 7 a.m.. Total 27,985 . 768 21.5 256. 3S.1 294.1 272.6 - 57.4 215.2 108,830 88.7 1,015.0 162. 6 1,177.6 ;l,088.9 +566.0 1,654.9 Total. 3 days 323.378 266. 2 3, 008. 4 479.3 3,487.7 3,221.5 +1,660.9 4,882.4 104 Table 122. — Summary of oal&rimetric measurements — Metabolism experiment? Nos. ?9 and 31. Date. Period. (a) Heat measured in terms. of Cao- (6) Change of tem- perature of calo- rimeter. (c) Capacity correc- tion of calorim- eter, 6x60. {&) Correc- tion due to tem- perature of food and dishes. 8 (e) Water vaporized equals total amount exhaled leas amount con- densed in chamber. (/) Heat used in vaporiza- tion of water. ex 0.592. {g) Total heat de- termined, 0+C+ d+f. 1900. Mar. 16-17 Experiment No. 29. 7 a.m. to 1 p.m... 1 p.m. to 7 p.m... 7 p.m. to 1 a.m... 1 a.m. to 7 a. m. .. Total 7 a.m. to 1 p.m... 1 p.m. to 7 p.m... 7 p. m.tol a.m... 1 a.m. to 7 a.m... Total 7 a. m. to 1 p.m... 1 p.m. to 7 p.m... 7 p. m.tol a.m... 1 a.m. to 7 a.m... Total Total, 3 days Experiment No. 31. 7 a. m.tol p.m... 1 p.m. to 7 p.m... 7 p.m.to7 a.m... 1 a.m. to 7 a.m... Total 7 a.m. tol p.m... 1 p.m. to 7 p.m... 7 p.m. tol a.m... 1 a.m. to 7 a.m... Total 7 a.m. to 1 p.m... 1 p.m. to 7 p.m... 7 p.m. tol a.m... 1 a.m.to7 a.m... Total Total. 3 days C.lni i:s, 1,113.0 1,139.0 4'.'.\:; 2.50.1 Degree. +0.02 + .09 + .01 - .01 Catorics. + 1.2 + 5.4 + .6 + .6 Calories. + 1.7 - 2.6 + 6.3 Grams. 280.6 288.9 276. 7 266.6 Calories. 166.1 171.0 163.8 157.8 Calories. 1 . 282. 1.312.8 666.0 408.5 2,997.4 + .13 + 7.8 + 5.4 1,112.8 658.7 3,669.3 17-18 1,074.3 1.078.3 414.1 216.3 + .01 + .05 + .03 + .6 + 3.0 + 1.8 + 2.8 - 4.7 + 5.4 260. 8 288.1 268.4 261.1 154.4 170.6 158.9 154.5 1 . 232. 1 1,247.2 578.4 372.6 2, 783. + .09 + 5.4 + 3.5 1,078.4 638. 4 3.430.3 18-19 1,116.2 1.195.8 468.5 208.0 + .04 + .11 + .02 - .03 - 2.4 + 6.6 + 1.2 - 1.8 + 7.2 - 7.0 + 5.8 274. 1 304.7 284.5 256. 8 162. 2 180.4 168. 4 152. 1 . 288. 1,375.8 643. 9 361.8 2, 988. 5 + .20 +12.0 + 6.0 1,120.1 663. 3, 669. 5 8, 768. 9 +25.2 + 14.9 3,311.3 1,960.1 10. 769. 1 Mar. 22-23 1,082.2 1,087.6 405. 9 221.9 + .01 - .6 + .6 + 3.1 + 3.1 277. 273. 7 260.4 252.5 164.0 162.0 1.54.1 149. •"> 1.248.7 1 . 245. 5 563.1 372. 2. 797. 6 + 2.1 1,063.6 629. 6 3. 429. 3 23-24 1,058.7 1.103.K 394.1 224. 3 + .01 + .02 4- .01 - .6 + 1.2 + .6 + 1.5 - 5.6 + .8 268. 5 266. 7 269. 5 264. 3 159.0 157. 9 159. 5 156. 4 1,219.8 1.257.3 554. 4 381.3 2, 780. 9 + .04 + 2.4 - 3.3 1,069.0 632. 8 3. 412. B 24-25 1.071.0 1, 094. 6 378.5 218.1 + 1.1 + 2.3 + 4.9 270. 5 284 1 264.5 272. 2 160.1 168.4 156.6 161.0 1,232.2 + .01 - .01 + .01 + .6 - .6 - . 6 1,265.9 539.4 379.7 2, 762. 2 + .01 + .6 - B.3 1,091.6 646. 1 3.417.2 8. 340. 7 + 3.0 + 7.1 3.224.2 1,908.5 10. 259. 3 a Including correction for 4.8 calories introduced during each day period by the current used to magnetize the fields of the dynamo. 105 Balance of income and outgo of matter and energy, — The income and outgo of nitrogen, carbon, hydrogen, and energy in experiments Nos. 29 and 31 are shown in Tables 123-126. Table 123. — Income and outgo of nitrogen and carbon — Metabolism experiments Nos. 29 and 31. Nitrogen. Carbon. Date. (a) In food. (&) In feces. (0 In urine." (d) Gain (+)or loss -16- (0 In food. (/) In feces. (9) In urine. (ft) In res- pira- prod- ucts. (*) Gain (+)or loss -(f+9 +h). 1900. < riment No. 29. Grams. Grams. Grams. Grams. Grams. Grams. Grams. Grams. Grams. Mar. 16-17, 7 a.m. to 7 a. m . . 16.0 0. 9 15. 4 -0.3 333. 6 8.3 10. S 338. 7 -24. 2 17-18, 7 a. m. to 7 a.m .. 16.1 16.3 -1.0 333. 7 B.4 11.4 323. 3 - 9.4 18-19, 7 a.m. to 7 a.m .. 16.0 . 9 16. 2 -1.1 333. 6 8.3 11.3 342.7 -28.7 Total. 3 days 48.1 2. 6 47. 9 -2.4 1.000.9 25. 33. 5 1.004.7 -62. 3 Average. 1 day 16.0 16.0 - .8 333. 6 8.3 11.2 334.9 -20.8 Experiment No. 31. Mar. 22-23, 7 a. m. to 7 a. m . . 16.1 16.3 -1.0 321. 5 8.1 11.3 313.1 -11.0 23-24,7 a.m. to 7 a.m.. 16.0 15. 4 321. 6 8.1 10.8 316.6 -13.9 24-25, 7a.m. to 7a.m.. 16.1 . 8 15. 2 - .1 321.5 8.1 10.6 317.8 -15.0 Total. 3 days 4s. 2 2.4 46.9 -1.1 964.6 24. 3 32. 7 947. 5 -39.9 Average. 1 day 16.1 15. 6 - .3 321. 5 8.1 10.9 315. 8 - 13.3 a Including nitrogen in perspiration, amounting to 0.2 gram per day. Table 124.— -Income and outgo of water and hydrogen — Metabolism experiments Nos. 29 and Si. Date. 1900. Experiment No. 99. Mar. 16-17. 7 a. m. to 7 a. m... 17-18, 7 a. m. to 7 a. m... 18-19, 7 a. m. to 7 a. m.. Total. 3 days Average. 1 day Experiment No. 31. Mar. 22-23, 7 a. m. to 7 a. m.. 23-24. 7 a. m. to 7 a. in.. 24-25. 7 a. m. to 7 a. m.. Total, 3 days Average. 1 day Water. («) (6) In food. In drink. Grams. 915. 5 915. 5 915. 5 Grams. 1.2-50 1.2-50 1.2.50 (o) (d) {e) (/) Apparent T Inres - l<»ss, In feces. In urine, piratorv a+b— product's. Grams. 41.2 41.3 41.2 Grams. 641.0 720. 3 834. 3 Grams. 1,667.5 1,439.4 1,736.5 Grams. 184.2 35. 5 446. 5 106 Table 124. — Income and outgo of water and hydrogen — Metabolism experiments Nos. 29 and SI — Continued. Hydrogen. Date. (9) In food. In feces. (0 In urine. (I). Apparent gain, g- (A+& (TO) Loss from wa- ter/-9. (n) Total gain ( + ) or loss (-)l+m. 1900. Experiment No. ~9. Grams. Grams. Grams. Grams. Grams. Grams. Mar. 16-17. 7 a. in. to 7 a. m 50.5 1.2 2.9 + 48.4 -20. 5 - 17-18. 7 a. m. to 7 a. m 50.5 1.2 3.0 - 16.3 - 3.9 -42.4 18-19, 7a. m. to 7 a. in 50.5 1.2 3.0 + 46.3 -49.6 - 3.3 Total. 3 days 151. 5 3. 6 8.9 + 139.0 -74.0 Average. 1 day 50. 5 1.2 3.0 + 46.3 -24. 6 -21.7 Experiment No. SI. Mar. '22-23. 7 a. m. to 7 a. m 48.8 1.1 3.0 - 44.7 -28.6 +16.1 23-24, 7 a. m. to 7 a. m 48, 8 1.2 i. - 44.7 -21.3 ^23. 4 24-25. 7 a. in. to 7 a. m 48.8 . 1.1 2.8 - 44.9 38.5 — 6.4 Total. 3 days 146.4 3.4 8.7 +134.3 -88.4 +45.9 Average, l day 48. 8 1.1 2.9 - 44.8 -29.5 +15.3 Table 125. — Gain or lass of protein (XX6.25), fat, and water — Metabolism experiments Nos. 29 and 31. Date. (o) Nitrogen gained ( + )or lost (--). (b) Protein gained ( + )or lost (-), ax 6.25. [€) Total carbon gained ( + )or lost (-). id) Carbon in protein gained ( + )or lost | - i, 6x0.53. Carbon in fat. etc.. gained 1 - i or lost (-). c-d. if) Fat gained ( + ) rate Time. ject in ™i e SSL ■£* Tempera- ture. Weight of sub- Time, ject in under- clothes. Pulse rate Tempera- per ture. minute. 1900. Experiment No. 3t. ,- Apr. 20. 7 a. m 66. 19 10 a. m 66 °F. 97.8 1900. Experiment . Apr. 26. 7 a. m 10 a. m Kgs. 64. 94 : F. 68 97. 7 102 65 97.7 66 97. 7 4 p. m 87 7 x>. m 66. 95 72 10 p. m 62 m; 96. 6 7 p. m 65.44 10 p. m 77 97.7 69 97. 2 Apr. 21. Til. m 66. 36 10 a. m 64 ' - -^ Apr. 27. 7 a. m 10 a. m 1 p. m 4 p. m 7 p. m 10 p. m Apr. 28, 7 a. m 10 a. m 1 p. m 4 p. m 7 p. m 10 p. m. 65.09 65. 34 61 98 65.37 65 97. 6 98 1 p. m 4 p. m 7 p. m 66. 27 10 p. m Apr. 22, 7 a. m 10 a. m 67 97 98.0 71 9s. 3 99 74 66 - 96 e 104 79 67 97.9 96.6 97.7 97.7 97.7 96.9 73 97. 7 97.3 67 97. 9 98 1 p. m 4 p. m 7 p. m 65.59 10 p. m 66 97. s 102 73 97. 69 97. 3 110 Amount of work done. — The total number of miles registered by the cyclometer and the heat equivalent of the work done each day are shown in Table 130. Table 130. — Record of work done — Metabolism experiments Nos. H2 and 34. (a) (6) (c) Time. Cyclometer reading. Number of miles. Actual duration of work. Rate. Heat equivalent, axbx 0.2378. 1900. Experiment No. 82. Seconds. Watts. Calories. 1,510.4 1,527.2 20. 10.15 a. m 16.8 7,200 18.8 32 20, 12.30 p. ra 1,546.5 19.3 7,200 21.0 36 20, 4 p. m 1,562.8 16.3 7,200 16.7 29 20, 6.15 p. m 1,579.1 16.3 7,200 17.4 30 Total 68.7 28, 800 127 Apr. 21, 10.15 a. m 1,599.2 20.1 7,200 21.0 36 21, 12.30 p. m 1, 626. 26.8 7,200 25.8 44 21, 4 p. m 1,654.0 28.0 7,200 30.5 52 21, 6.15 p. m 1,681.7 27.7 7,200 29.6 51 Total 102. 6 28, 800 183 Apr. 22, 10.15 a. m 1,711.6 29.9 7,200 36.2 62 22, 12.30 p. m 1,744.6 33.0 7,200 47.4 81 22, 4 p. m 1,774.5 29.9 7,200 38.1 65 22, 6.15 p. m 1, 806. 1 31.6 7,200 40.0 69 Total 124.4 28, 800 277 Total, 3 davs 295. 7 86, 400 587 Experiment No. 3£. Apr. 26, 10.15 a. m 2, 166. 9 28.9 7,200 34.3 59 26, 12.30 p. m 2, 196. 7 29.8 7,200 35.4 60 26, 4 p. m 2,226.1 29.4 7, 200 34.3 59 26, 6.15 p. m 2, 254. 1 28.0 7,200 33.7 58 Total 116.1 28,800 236 Apr. 27, 10.15 a. m 2, 283. 8 29.7 7,200 35.7 61 27. 12.30 p. m 2, 318. 7 34.9 7,200 36.8 63 27, 4 p. m 2, 347. 6 28.8 7,200 38.1 65 2,379.0 31.5 7, 200 38.7 66 Total 124.9 28, 800 Apr. 28. 10.15 a. m 2, 409. 6 30.6 7,200 37.4 64 28, 12.30 p. m 2,441.5 31.9 7,200 38.1 65 28, 4 p. m 2, 472. 6 mi 7,200 38.1 65 28, 6.15 p. m 2, 503. 8 31.2 7,200 38.1 65 Total 124.8 28, 800 259 Total, 3 davs 365.8 86, 400 750 Ill Detailed data of inconu and 'outgo, — The quantities of nutrients in the basal ration and the quantities in the supplemental rations for the two experiments are shown in Table 131 and the outgo of matter and energy in the feces in Table 132. Table 131. — Weight, composition, and heat of combustion of foods — Metabolism experiments Nos. 32 and 34- Lab- ora- tory No. 3205 3206 3204 3207 3193 Food material* Weight WatpT perday. Water - Pro- tein. Fat. C ^°- Xitro- diates. S en - Beef Butter Bread Ginger snaps... Parched cereal Sugar Grams. 58 35 300 35 lira in*. 37.4 2.9 113.4 2.8 3.1 Gram*. Grams. 18.6 .4 25. - 4.1 9.0 1.6 30. 7 5.4 1.1 Grams. Grams. 2. 98 .07 149.4 4.14 61.2 60.4 1.44 35.0 ! Basal ration, exclu- sive of milk 57.9 46.3 306.0 9.29 EXPERIMENT NO. 32. Car- bon. Hydro- gen. Heat of bus- tion. Gram.<. 10.76 22. 95 -4.-1 32.90 32.04 14.74 Grams. 1.-54 3.63 12.90 5.40 4.73 2. 27 Calo- ries. 120 287 861 333 315 139 198.20 30.47 2.055 321X1 Milk, whole Total basal ration.. Butter < supplemental ration i Total ration, lday.. EXPERIMENT NO. 34. Basal ration, exclu- :' milk 1.020 871.1 41.8 51.0 47.9 6.73 81.09 12. 14 923 3206 1.59s 62 1.030.7 5.2 99.7 .8 97.3 353.9 16.02 .12 279. 29 40.66 42. 61 6.43 2, 97s ■509 1.660 1.035.9 100.5 151.6 353.9 16.14 319.95 49.04 3.4S7 578 1.O20 159. 6 869.0 57.9 41. s 46.3 53. 306.0 43.9 9.29 6.73 198.20 83.64 30.47 12.34 2, 055 3202 Milk, whole Total basal ration.. Sugar | supplemental ration | 'Total ration, lday.. 931 1 . 598 12 - 1,028.6 99.3 349.9 128.0 16.02 281.84 53.89 42. 81 8.29 _ 10 507 1.726 1.028.6 99.7 99.3 477.9 16.02 335.73 51.10 3.493 Table V.V2. — Weight, composition, and heat tf combustion of feces — Metabolism experiments Nos. 32 and 34. Lab- ora- tory num- ber. Weight of feces. Water. Pro- tein. Grams. 22. 3 7.4 Carbo- Fat. hy- drates. Nitro- gen. Car- bon. Hydro- gen. Heat of combus- tion. Experiment No. .>.'. Total. 3 day- Average. 1 day ... Grams. 293. 3 97. 8 Grams. 214. 7 71.6 Grams, drams. 13.2 28.2 4.4 9.4 Grams. 3.55 1.1s Grams. 37. 75 12. 58 Grams. 5.46 1.-2 calories. 425 142 Experiment No. 54. 3211 Total, 3 days.... Average, 1 day .. 179. 9 B5. 3 60. 22.0 14. s 23. 3 34.70 4.94 4.9 7.- 1.1s 11.57 1.65 377 126 112 The statistics of the quantity and composition of the urine eliminated on different days and different periods of the day are shown in Tables 133 and 134. The statistics of measurements of carbon dioxid and water in the ventilating air current for 6-hour periods are given in Tables 135-137. Table 133. — Amount, specific gravity, and nitrogen of urine by 6-hour periods — Metabo- lism experiments Nos. 82 and 84. Date. Period. Amount of urine. Specific gravity. Nitrogen content. 1900. Apr. 20-21 Experiment No. 32. Grams. 279.6 371.1 324.8 262. 1 1.026 1.022 1.018 1.018 Per cent. 1.41 1.26 1.21 1.28 drams. 3.94 4.68 7 p. m. to 1 a. m 3.93 3 35 Total 1,237.6 1,237.6 15 90 Total by composite 7 a. m. to 1 p. m 1 p. m. to 7 p. m 7 p. m. to 1 a. m 1 a. m. to 7 a. m Total Total by composite 1.021 1.28 15. 84 21-22 446.6 586.5 240.0 214.8 1.015 1.015 1.022 1.021 .85 .74 1.50 1.49 3.80 4.34 3.60 3.20 1,487.9 1,487.9 14.94 1.018 1.01 15.03 22-23 404.3 358. 2 192. 5 149.1 1.020 ' 1.023 1.030 1.029 .94 1.20 2. 02 2. 15 3 80 1 p. m. to 7 p. m 7 p. m. to 1 a. m 1 a. m. to 7 a. m Total Total by composite Total, 3 days, by periods Total by composite 4.30 3.89 3. 21 1,104.1 1,104.1 15. 20 1.024 1.39 15.35 3,826.6 3,829.6 46.04 46.22 Experiment No. 9k. Apr. 26-27 243.4 271.2 192.7 144.1 1.027 1.029 1.030 1. 029 1 . 75 1.83 2. 29 ■1. 35 4.26 1 p. m. to 7 p. m 4.96 7 p. m . to 1 a. m 4.41 1 a. m. to 7 a. m Total Total by composite 7 a. m. to 1 p. m 1 p. m. to 7 p. m 3.39 851.4 851 . 4 17.02 1.030 1.98 16.86 27-28 257.2 301.6 200.1 150. 1 1.024 1.027 1.029 1. 028 1.59 ,.« 1.93 2. it; 4.09 4.67 7 p. m. to 1 a. m la. m. to 7a. m Total Total by composite 7 a. m. to 1 p. m :.;. 86 3.24 909.0 909.0 15. 86 1.026 ] . 70 15.45 28-29 280.4 £56. 3 302. 5 156. 2 1.025 1.024 1.021 1.027 1.43 1.28 1.44 1.99 4.01 4. 56 7 p. m. to 1 a. m 4.36 1 a. m. to 7 a. m 3.11 Total Total by composite Total, 3 davs, by periods 1,095.4 1.095.4 16.04 1.024 1.45 15.88 2. 855. S 2, 855. 8 48. 92 Total bv composite 18. 19 Total, 9 days, » by composite 9.910.0 1.024 1.46 144.79 Including 3 days of an experiment not here reported. 113 Table 134. — Daily elimination of carbon, hydrogen, miter, and energy in urine — Metabo- lism , xperimenU Nos. 32 and 34. Date. Amount Heat of com- bustion. Period. Qf urine Per gram. Total. 1900. Apr. 20-21 21-22 22-23 Experiment No. 3f. 7 a. m. to 7 a. m... 7 a. in. tn 7 a. m... 7 a. ru. io 7 a. m... Grains. 1,237.6 1,487.9 1,104.1 ' 1 P.et. Grams. P.ct. 11.35 ...... 10.67 10.85 Grams. 3.13 2.95 3.00 P.ct. Grams. 1, 179. 7 1,433.4 1,048.7 Calorie. 0.104 .076 .105 Calories. 129 113 116 Total, 3days. 3.>2y.6 32.87 9.08 3.661.8 358 Apr. 26-27 27-28 28-29 Experiment No. $U. 7 a. m. m 7 a. m... 7 a. m. tn7a.ni... 7 a. m. to 7 a. m... Total. 3 days. Total, 9 days". 851 . 4 909.0 1,095.4 12.15 I 11.32 11.45 3.36 3.13 3.16 789.4 851.2 1,036.9 .154 .137 .112 131 125 123 2,855.8 34.92 9.65 '2,677.5 379 ! 9,910.0 1.05 104.06 0.29 28.74 94.64 9, 378. 8 .111 1,090 a Including 3 days of an experiment not here reported. 1300T— Xo. 109—02 8 114 Table 135. — Comparison of residual amounts of carbon dioxid and vmter in the chamber at the beginning and end of each period, and the corresponding gain or loss — Metabolism experiments Xos. 82 and 34- End of period. Carbon dioxid. Water. Pate. Total amount in chamber. Gain ( + ) or loss (-) over preced- ing period. Total amount of vapor remain- ing in chamber. Gain ( + ) or loss (— ) over preced- ing period. Change in weight of ab- sorbers, a gain i -r 1 or loss ( — ) . Drip from absorb- ers. Total amount gained ( + )or Lost 1 -) during the period. 1900. Apr. 20 20-21 Experiment No. 32. Grams. 27.0 70.7 64.6 27.6 27.6 Grams. Grams. 39.0 49.0 48.6 44.5 43.1 Grams. Grams. Grams. Grams. +43.7 - 6.1 -37.0 + 10.0 - .4 - 4.1 - 1.4 -127 + 30 — 55 - 55 35. 7 130.0 8.0 8.0 + 172. 7 + 159. 6 1 a . in 7 a. m Total - 51.1 4> 4 + .6 + 4.1 + 47 181.7 232 8 21-22 83.4 86.6 27.2 30.4 +55.8 + 3.2 -59.4 + 3.2 ■2. 3 52.6 46.2 49.4 - 9.2 + .3 - 6.4 + 3.2 + 190 + 12 - 86 - 85 94.0 424. B 28.0 28.0 + 293. 2 7 p. m la. m 7 a. ra Total 1 p. m + 437. 1 - 64.4 - 53. 8 + 2.8 + 6.3 + 31 574. 8 + 612. 1 22-23 92.5 86.8 28.5 27.0 +62.1 - 5.7 -58.3 - 1.5 58.1 53.8 49.6 46.7 + 8.7 - 4.3 - 4.2 - 2.9 +192 - 26 - 70 - 70 334.8 595.0 40.0 40.0 7 p. m + 564. 7 34 2 — 32.9 Total - 3.4 - 2.7 + 26 1,009.8 - 1 - 1,033 1 Experiment No.SU. Apr. 26 26-27 26.0 81.3 78.3 28.1 26.2 46.0 54.9 53.4 47.9 46.2 1 p. m + 55.3 - 3.0 -50.2 - 1.9 + 8.9 - 1.5 - 5.5 - 1.7 +184 - 19 - 72 - 71 231.5 433.2 11.0 11.0 - 424. 4 7 p. m + 412. 7 1 a. m - 66.5 61 7 Total + .2 + 22 686. 7 + 708. 9 1 p. m 27-28 82.1 74.5 25.3 25.8 +55.9 - 7.6 -49.2 + .5 54.7 53.0 44.5 45.0 + 8.5 - 1.7 - 8.5 + .5 +155 - 6 - 84 - 84 247.2 461.8 18.0 18.0 + 410. 7 - 454. 1 — 74.5 1 a. m 7 a. m — 65. 5 Total - .4 — 1.2 - 19 745.0 - 724.8 1 p. m 28-29 81.9 78.1 26.0 25. 1 +56.1 -52. 1 - .9 53.6 52.4 46.4 43.5 + 8.6 - 1.2 - 6.0 - 2.9 +177 - 78 - 78 215. 6 456. 3 13.0 13.0 - 401.2 7 p. m + 448. 1 1 a. m - 71.0 7 a. m - 67.9 Total - • ? - 1.5 - 14 697. 9 + 710. 4 * Absorbers were not weighed between 7 p. m. and time is divided equally between the two periods. a. m. The change in the weight during this 115 Table 136. — Record of carbon dioxid in ventilating air current — Metabolism experiments Xos. S3 and 34. (a) Carbon diox d. (h) Period. - a a t flSJ si's > In incoming air. be c Total excess in outgoing ~T air, d—c. Correction for amount re- q mainlng In 3" chamber. (9) - v ~ ~ z- ~ '- 3*0 w oil® OT3 Date. (6) 1 (c) o e 'I - 1 O o Ca Eh 1900. Experiment No. Si. Liters. Mg. Grams. Grams. Grams. Grams. Grams. Grams. Apr. 20-21 7 a.m. to 1 p.m.. 25, 652 0.559 14.3 350.9 336.6 +43.7 380.3 103.7 1 p.m. to 7 p.m.. 26,430 .620 16.4 393.9 377.5 - 6.1 371.4 101.3 7 p.m. to 1 a.m.. 27, 985 .608 17.0 243. 9 226. 9 -37.0 1S9.9 51.8 1 a. m. to 7 a.m.. Total 7 a.m. to 1 p.m.. 27,208 .675 18.4 157.1 : 138.7 138.7 37.8 107, 275 66.1 1,145.8 1,079.7 + .6 1,080.3 294.6 21-22 25, 952 . 675 17.5 385.1 367.6 +55.8 423. 4 115.4 1 p.m. to 7 p.m.. 25, 952 .640 16.6 472.8 456. 2 + 3.2 459.4 125. 3 7 p.m. to 1 a.m.. 26, 430 .583 15.4 257. 2 241.8 -59.4 182. 4 49.7 1 a.m. to 7 a.m.. Total 7 a.m. to 1 p.m.. 27, 208 .614 16.7 158.3 141. 6 + 3.2 144. S 39.5 1G5, 542 66.2 1,273.4 1,207.2 + 2.8 1,210.0 329.9 22-23 26, 730 .590 15.8 439.9 424.1 +62.1 486. 2 132.6 1 p.m. to 7 p.m.. 25, 952 .599 15.5 499.6 484.1 - 5.7 478.4 130.4 7 p.m. to 1 a.m.. 26, 430 .599 15.8 267.7 251.9 -58.3 193.6 52.8 1 a.m. to 7 a.m.. Total Total, 3 days Experiment No. $U. 27, 208 .601 16.3 151.9 135.6 -1.5 134.1 36.6 106, 320 63.4 1,359.1 1,295.7 - 3.4 1,292.3 352.4 319. 137 195.7 3,778.3 3,582.6 3, 582. 6 976.9 Apr. 26-27 7 a.m. to 1 p.m.. 26, 730 . 582 15.6 418.1 402. 5 +55.3 457.8 124.8 1 p.m. to 7 p.m.. 27,508 .578 15.9 483.8 467.9 - 3.0 464.9 126. 8 7 p.m. to 1 a.m.. 27, 208 .575 15.6 252.4 236.8 -50. 2 186.6 50.9 1 a.m. to 7 a.m.. Total 7 a.m. to 1 p.m.. 27, 208 .620 16.9 150.7 133.8 - 1.9 131.9 36.0 108, 654 64.0 1,305.0 1,241.0 + .2 1,241.2 338.5 27-28 28, 285 . 568 16.1 440.0 423.9 +55.9 479.8 130. 9 1 p.m. to 7 p.m.. 27,507 . 582 16.0 499.0 483.0 -7.6 475. 4 129.7 7p.m. to 1 a.m.. 29,540 .580 17.1 262. 244. 9 -49. 2 195.7 53.3 1 a.m. to 7 a.m.. Total 7 a.m. to 1 p.m.. 28,762 .587 16. 9 152. 1 135.2 + .5 135.7 37.0 114, 094 66.1 1,353.1 1,287.0 - .4 1,286.6 350.9 28-29 27, 985 .561 15.7 434.5 418.8 +56.1 474.9 129. 5 1 p.m. to 7 p.m.. 27,985 .553 15. 5 498. 482. 5 - 3.8 478.7 130.6 7 p.m. to 1 a.m.. 28, 762 .593 17.1 256.7 239. 6 -52.1 1-7.5 51.1 1 a.m. to 7 a.m. . Total Total, 3 days 29, 540 .632 18.7 150.5 131. 8 - .9 130.9 35.7 114, 272 67.0 1,339.7 1,272.7 - -7 1,272.0 346.9 337, 020 1 197.1 3,997.8 3, 800. 7 3, 799. 8 1,036.3 116 Table L37. —Record of water in ventilating air current — Metabolism experiments Nos. 38 and .;;. Period. (a) = '- z L - - I- - > Water in incoming air. Water in outgoing air. (9) is H - " z : ■-'- -"3 c £ t~ o - - o (0 Date. (b) (c) ■d X s "5 (d) ~ / ^ - - N - - w < (e) - ' . - = X = = — < (/) a + - Total water of r, piration and p» spiration, // + //. 1900. Experiment No. SS. //7m> . Mgs. Grams. Grams. Grams. Grams. Grams. Grams. Apr, 20-21 7 a. m. to 1 p. m.. 25, 652 0.999 207.8 45.6 253.4 227. - 172.7 400.5 1 p.m. toT p. in. . 26, 430 .972 25. 7 225.6 43.3 268.9 243.2 +159.6 402.8 7 p.m. to 1 a.m.. 27,985 .936 26.2 229.6 44.6 271.2 248.0 - 51.1 196.9 1 a.m. to 7 a.m.. Total 7 a.m. to 1 p. m.. 27,208 .859 23.4 231.0 37.5 268.5 245.1 - 48.4 196.7 107.275 100. 9 894.0 171.0 1.065.0 964.1 +232.8 1,196.9 21-22 25,952 1.041 27.0 222. 1 46.7 269.1 242.1 +293.2 535. 3 1 p.m. to 7p.m.. 25,952 1.060 27.5 213.4 51 . 8 265. 2 237.7 i:;7. 1 674.8 7 p.m. to 1 a. m. . 26,430 .886 23. 4 241.2 11.2 285. 1 262.0 64.4 197.6 1 a. m. to 7 a. m.. Total 7 a.m. to 1 p. m.. 27,208 1.818 22.2 240. 2 278. 7 256. 5 - 53". 8 202.7 105,542 100.1 917.2 18L2 L.098.4 998. :'» +612.1 1,610.4 22-23 26, 730 1.085 29.0 252. 48.5 300.5 271.5 807.0 1 p.m. to 7 p.m.. 25,952 1.169 30.3 260. 1 46.8 306.9 276. 6 841.3 7 p. m. to 1 a.m.. 26,430 1.022 27.0 241. 7 42.0 283.7 2.56. 7 - 34.2 222. 5 1 a.m. to 7 a.m.. Total 27,208 .879 23.9 241.0 39.3 280.3 256. I - 32.9 223. 5 106,320 110.2 994. 8 176.6 1,171.4 1,061.2 +1,033.1 2,094.3 Total, :](lays 319, 137 311.2 2. 806. 528. 8 3,331.8 3,023.(1 1,878.0 4,901.6 Experimt »i No. • •",. Apr. 26-27 7 a.m. to 1 p.m.. 26, 7:50 .910 24.3 248.2 44.4 292.6 268. 3 +424.4 692.7 1 p.m. to 7 p.m.. 27,508 .973 26.8- 261.6 46.0 307.6 280.8 +412.7 693.5 7 p.m. to 1 a.m.. 27, 208 .859 23.4 239.3 40.6 279.9 256. 5 - 66.5 190.0 1 a.m. to 7 a.m.. 27,208 . 776 21.1 246.8 35.6 282. 4 261.3 - 61.7 199.6 Total 7 a. m. to 1 i>. m.. 108, 6.54 95.6 995. 9 166.6 1,162.5 1,066.9 +708.9 1,775.8 27-28 28, 285 .938 26. 5 2.50.2 45.6 295. 8 269.3 +410.7 680.0 1 p.m. to 7 p.m.. 27,507 1.004 27.6 271.4 42.5 313.9 286.3 1-454.1 740.4 7 p. in. to 1 a.m.. 2^,540 .827 24.4 254.0 46.1 300.1 27:.. 7 - 74.5 201.2 1 a. m. to 7 a. m.. Total 7 a.m. to 1 p. m.. 28,762 .764 22.0 250.6 39.6 290.2 268.2 202.7 114,094 100. 5 1,026.2 173. 8 1.200.0 1,099.5 -724.8 1,824.8 28-29 27, 985 . 927 25.9 253. 5 46.7 300.2 274.3 +401.2 675. 5 1 p.m. to 7 p. m.. 27,985 1.031 28.8 269.0 46.3 315.3 286.5 +448. 1 7: '.1.6 7 p.m. to 1 a.m.. 28, 762 .904 26.0 246.0 45.9 291.9 265.9 - 71.0 194.9 1 a.m. to 7 a. in.. 29,540 .828 24.4 253. 3 U.6 294.9 270.5 - 67.9 202. 6 Total Total, 3 days 114,272 105. 1 1,021.8 180.5 1.202.3 1,097.2 + 710.4 1,807.6 337, 020 301.2 3,043.9 i 520.9 3,564.8 <. 263. 6 2,144.1 5, 407. 7 117 Table 138 shows the amount of heat given off during the successive 6-hour periods of the two experiments. Table 138. — Summary of calorimetric measurements — Metabolism experiment* Nos. 82 .0 3.6 16.0 1.2 17.4 16. 3 16.4 I -2.6 -1.5 1.6 960.0 320. 37. 8 12. 6 32.9 11.0 976.9 -87.6 325. 6 -29. 2 335.7 335.8 335.7 11.6 11.5 11.6 12.2 11.3 11.4 .50.1 16.7 5.7 1.007.2 1.9 335.7 34.7 11.6 34.9 11.6 338. 5 -26. 6 350. 9 -37. 9 346.9 -34.2 1,036.3 345. 4 -98.7 32.9 a Including nitrogen in perspiration, amounting to 0.4 gram per day. Table 140. — Income and outgo of water and hydrogen — Metabolism experiments Xos. 3? and 34. Water. Date. (a) In food. In drink. (c) In feces. (d) In urine. (c) respira- tion prod- ucts. (/) Appar- ent loss, ai-b- (c-fd+e). 1900. Experiment No. .52. Grams. Grams. Grams. Greuns. Grams. Grams. Apr. 20-21, 7 a. m. to 7 a. m 1 , 035. 9 1,250 71.6 1,179.7 1,196.9 162.3 21-22, 7 a. m. to 7 a. m 1.035.9 1,250 71.5 1.433.4 1,610.4 829. 4 22-23. 7 a. m. to 7 a. m 1.035.9 1,250 71.6 1.048.7 2, 094. 3 '.'2-. 7 Total. 3 days 3,107.7 8. 750 214.7 3,661.8 4,901.6 1 . 920. 4 Average. 1 dav 1,035.9 1.250 71.6 1,220.6 1, 633. 8 640. 1 Experiment No. 34. Apr. 26-27, 7 a.m. to 7 a. m 1,028.6 1,2-50 60.0 7-9. 4 1,775.8 346.6 27- v 7 a. m. to 7 a. m 1,028.6 1.250 1,2.50 59.9 851.2 1,824 3 456. ^ 28-29, 7a.rn.to7a.rn 1,028.6 60.0 1,036.9 1,807.6 625. 9 Total. 3 dav- 3,085.8 1,028.6 3 750 179 9 2.677.5 ^92. 5 5. 407. 7 1.429.3 Average. 1 'lav 1.250 60.0 1.-02.5 476.4 119 Table 140. — Income and outgo of water and hydrogen- and 34 — Continued. -Metabolism experiments Xos. 32 Date. Hydrogen. (flO (h) In food. In feces. (0 In urine. (0 Appar- ent gain, g-(h+i), (m) Loss from water, /-9. (») Total gain ( + ) or loss ( — ), l+m. 1900. E.rjK riment No. 32. Apr. 20-21 .7 a. m. to 7 a. m 21-22, 7 a. m. to 7 a. m 22-23, 7 a. m. to 7 a. m Total, 3 days... Average. 1 day. Experiment No. Si Apr. 26-27, 7 a. m. to 7 a. rn . . . 27-28,7 a.m. to 7 a.m. 28-29, 7 a.m. to 7 a.m. Total, 3 days Average. 1 day Grams. Grams. 49.0 1.8 49.0 1.8 49.0 1.8 Grams. 3.1 3.0 3.0 Grams. + 44.1 + 44.2 + 44.2 Grams. - 18.0 - 92.2 -103.2 147.0 49.0 5.4 1.8 9.1 3.0 +132. 5 -213. 4 + 44.2 - 71.1 51.1 51.1 51.1 1.7 1.6 1.7 153.3 51.1 5.0 1.7 3.4 3.1 3.2 46.0 46.4 46.2 9.7 3.2 +138. 6 + 46.2 38.5 50.8 69,5 -158. 8 - 52.9 Grams. +26.1 -48.0 -59.0 26.9 - 4.4 -23.3 -20. 2 - 6.7 Table 141. — Gain or loss of protein (X X6.25), fat, and water — Metabolism experiments Xos. 32 and 34. Date. (a) Nitrogen gained ( + )or lost(-). (6) Protein gained ( + )or lost(-), ax 6.25. (c) Total carbon gained ( + )or lost(-). (d) Carbon in pro- tein gained ( + )or lost(-), 6x0.53. (e) Carbon, in fat etc., gained ( + )or lost(-), c-cl. (/) Fat gained ( + )or lost (-), e^-0.761. 1900. Experiment Xo. 32. Apr. 20-21. 7 a.m. to 7 a. m 21-22, 7 a. m. to 7 a. m 22-23, ~r a . m. to 7 a . m Grams. -1.4 - .3 Grams. - 8.7 - 1.9 -4.4 Grams. + 1.5 -33. 2 -55.9 Grams. - 4.6 - 1.0 _ 93 Grams. + 6.1 -32.2 -53.6 Grams. + 8.0 - 42.3 — 70 4 Total . 3 davs -2.4 -15.0 - .8 1 - 5.0 -S7.6 - 7.9 _oo O _ 9 fi -79.7 — 9fi fi 104 7 Average, 1 dav — 34 9 Experiment No. SU. Apr. 26-27, 7 a.m. to 7 a. m — 2.6 i — 1fi.S -26.6 - S.6 37 9 5 -18.0 32 9 — 23 7 -1.5 -1.6 - 9.4 -10.0 43 2 7 a.m. to 7 a. m -34.2 5 3 9K Q 38 Total, 3 davs -5.7 -1.9 —35.7 —98 7 —18 9 -79.8 -26.6 104 9 11 9 3-> 9 « * 35 120 Table 141. — Gain or loss of protein (X X6.25), fat, and water — MetaboU^n experiments Nos. 82 and 34— Continued. Date. (<7) Total W Hydrogen hydrogen "* protein | in fat etc., gained gained < °^ gained + gained ( + ) (+ Sr lost Slo?U-S ) orlost(-).orlost (-), YJ) - I 6x0.07. /x 0.118. , k (£#) Hydrogen Hydrogen in W ater, in fat Water gained orlo-- A-X9. 1900. Experiment No. 32 Apr. 20-21, 7 a. m. to 7 a. m . 21-22. 7 a. m. to 7 a. m . 22-23. 7 a. m. to 7 a. m . Total, 3 days Average, 1 day Experiment No. Sh Apr. 20-27, 7 a. m. to 7 a. m . 27-28, 7 a. m. to 7 a. m . 28-29, 7 a. m. to 7 a. m . Total, 3 days Average, 1 day Grams. Grams. +26. 1 -0. 6 -48.0 - .1 -59.0 - .3 -26.9 + 7.5 -4.4 -23.3 -20.2 Grams. + 0.9 - 5.0 - 8.3 Grams. -25. 8 -42.9 -50.4 •1.0 -12.4 - 4.1 -67.5 -22. 5 -1.1 -2.8 -5.1 -4.5 -11.4 + 1.4 -18.1 -z. o - .8 -12.4 - 4.1 -5.3 -1.8 Grams. -232.2 — 453.6 -607. 5 +102.6 - 12.6 -162. 9 - 47.7 - 15.9 Table 142. — Income, and outgo of energy — Metabolism experiments Nos. 3? and 34- Date. (a) (6) (c) Heat of Heat of Heat of com- com- com- Esti- mated heat of com- bustion bustion bustion bustion of pro- of food of eaten, feces. («) Esti- mated heat of com- bustion Esti- mated energy of ma- terial 1900. Experiment Xo. 32. Apr. 20-21, 7 a.m. to 7 a.m. 21-22, 7 a.m. to 7 a.m. 22-23,7 a.m. to 7 a.m. Total, 3 day- Average, 1 day Experiment No. SU. Apr. 26-27, 7 a.m. to 7a.m. 27-28,7 a.m. to 7 a.m. 28-29, 7 a.m. to 7 a.m. Calo- ries. ; 3,487 3,487 3, 487 Calo- ries. 142 141 142 of tein SSlti dizedin mined. urine, gained ?**??£ the ( + )or ( + > or body, a lost , ios ? -(6+c| (-)• (-)• Calo- ries. 129 113 116 Calo- ries. - 35 3 - 11 - Calo- ries. 77 404 672 +d+e) Calo- 3,174 3,634 3,912 10,461 425 3.4-7 142 358 119 43 - 14 - 333 10, 720 10, 694 3, 573 3, 565 493 493 Total, 3 days... Average, 1 day 126 131 - 79 - 226 125 125 - 40 - 413 126 123 - 43 - 363 3. -541 3, 568 3, 6% 3, 6:33 3, 6-50 3, 561 10,479 3, 493 126 379 126 -162 1-1,002 10,887 10,762 - 54 - 334 3,629 3.587 - 27 - 63 -1.7 -2.4 125 42 1.1 121 SUMMARY AND RESULTS OF THE EXPERIMENTS. SCOPE OF THE INVESTIGATIONS. Number of experiments and experimental days. — Between February. 1896, and April. L900, inclusive, 34 experiments, covering a period of 114 days, were made with the respiration calorimeter. The tirst four of these, covering a period of 21 days, were desig- nated as experiments Nos. 1-1. and the results were published in a previous bulletin. 1 ' Nine of the 12 days covered by experiment No. 1, however, really comprised three separate experiments, distinguished from one another by difference in occupation of the subject: these, for convenience, may be designated as la. lb. and 1c. In all these experiments (Xos. l-4c) the income and outgo of nitrogen and carbon and the income of energy were determined, but the heat given off from the body was not measured. Since they show only the bal- ance of income and outgo of matter, including the material excreted in the breath, they are termed ;% respiration" experiments. The results of six other experiments. Xos. 5-10. covering a period of 24 days, have also been published. b In these, and all later experi- ments, the balance of income and outgo of nitrogen, carbon, hydrogen, and energy was determined, and they are. therefore, termed "respira- tion calorimeter" or •• metabolism" experiments. Of the remaining 24 experiments. 13, covering a period of 41 days, are reported in detail in the present bulletin: the results of the other 11 experiments, covering a period of 2^ days, and belonging to another investigation, are reported elsewhere. Accordingly, the total number of respiration calorimeter experi- ments thus far decribed. including those in the present report, is 30, covering 93 days. Besides these. 14 experiments, covering 41 days, made in the winter and spring of 1900-1901, are now completed and nearly ready for publication, thus making 44 experiments. Xos. 4-4S, covering 134 days, in which the balance of nitrogen, carbon, hydro- gen, and energy has been directly measured. Adding the respiration experiments, included in Xos. l-4c and covering 21 days, we have 48 experiments, covering 155 days, in which the balance of nitrogen and carbon was determined. Each metabolism experiment, or series of experiments, was imme- diately preceded by a digestion experiment of several days' duration, in which the subject had the same diet as in the metabolism experi- ment, the purpose being to bring the body into approximate nitrogen *U. S. Dept. Agr., Office of Experiment Stations Bui. 44. ! U. S. Dept. Aer.. Office of Experiment Stations Bills. 63 and 69. e Memoirs of the National Academy of Sciences, Vol. VIII. Sixth Memoir, 1902. 122 and carbon equilibrium before the beginning of the metabolism experi- ment proper, and at the same time to secure a more nearly accurate measure of the digestibility of the food. It is almost impossible to obtain exact nitrogen equilibrium since physiological factors, little understood and not easily controlled, cause more or less fluctuation in the elimination of nitrogen, even when the amount in the diet is uniform. These preliminary digestion experiments were generally of 1 days' duration. Experiments Xos. 1-4, however, were both pre- ceded and followed by digestion periods of 3 days, while some later experiments were preceded by periods more or less than 1 days. In these preliminary digestion experiments the income and outgo of nitro- gen were determined, so that the nitrogen metabolism was measured, and in this sense they are metabolism experiments, although they are not here so designated. There were altogether 14 digestion and nitro- gen metabolism experiments which accompanied or made a part of the 31 experiments with the respiration calorimeter. They covered 201 days. Only a small number of the results of these digestion experi- ments, viz, those belonging to respiration experiments Xos. 1-1, have yet been published. Questions studied.- — Although the larger part of the seven years' work in connection with the respiration calorimeter in this laboratory has been devoted to the developing and perfecting of apparatus and experimental methods, yet during the progress of the inquiry a mass of data has accumulated and results bearing upon a variety of ques- tions have been obtained. A proper discussion of the results would include a consideration of the following and other topics: (1) The kinds, amounts, and digestibility of the food; (2) the quantity and composition of the excretory products; (3) the daily elimination of water, carbon dioxid, and heat, and the rates of elimination at different periods of the day and under different conditions; (1) the kinds and amounts of material actually oxidized by the different subjects under different conditions of rest and work; (5) the estimated amounts of oxygen used and the estimated respiratory quotients; (6) the demands of different persons for nourishment under different conditions: (7) the functions of the different nutrients and their mutual replacing power, or the nutritive values of different food materials; (8) the energy of the food and of the material oxidized as compared with that given off from the body: and. finally (9), the general bearing of the results upon the metabolism of energy. A number of collateral topics would also call for discussion, such as (10) the effects of different conditions of food, rest, and work upon bodily temperature; (11) the composition of the air in the chamber as related to the rate of ventilation and the well-being of the occupant, and as bearing upon the general hygiene of ventilation, (12) details of the apparatus, and (13) methods of experi- mental inquiry. 123 While none of these topics pan be discussed to the best advantage until the publication of further experimental data, or without due con- sideration of the results of research elsewhere, brief reference may be made to a few of them in order to illustrate some of the ways in which the experiments are throwing light upon the fundamental questions of the metabolism of matter and energy in the body, and consequently upon the general laws of nutrition. Accordingly, the data already reported are here used for a preliminary discussion of a number of problems of general interest. These include (1) food materials -up- plied and consumed and the difference in the amounts of nutrients demanded by men at rest and at work. (2) the elimination of water. (3) the elimination of carbon dioxid. (4) the elimination of energy, and (5) the income and outgo of energy and the action of the law of the conservation of energy in the body. The text-books and treatises bear- ing on these subjects contain more or less detailed statements and conclusions. It appears that in numerous instances the commonly quoted figures and conclusions rest upon much less experimental evi- dence than- is furnished by the experiments with the respiration calo- rimeter just referred to. In the construction of this apparatus it was possible to make use of the experience and results of earlier investi- gators; the facilities have permitted the obtaining of more accurate and extensive data than had previously been practicable: and it is believed that the results are of sufficient interest to warrant their publication even though future research may call for more or less modi- fication of the inferences deduced from them. Brief summaries of results are given on the following pages. These results are derived from the statistics of experiments Nos. 5-11. 13, 14. 21. 23-26. 28, 29. 31. 32. and 34. The result.- summarized in Table 144. p. 129. include, in addition, the results of experiments Nos. 1-4. in which the outgo of energy was not determined. Subjects of the experiments. — Four different men. E. O., O. F. T., A. W. S... and J. F. S.. have served as subjects in these experiments. They were all in excellent health. E. O. was a laboratory assistant, a native of Sweden, who had been a number of years in this country. At the time of the experiments here recorded he was about 32 years old and weighed not far from 70 kilograms. A. W. S. was a phys- icist, a native of New England. 25 years old. and weighed about 70 kilograms. O. F. T.. a chemist, the subject of but one experiment, No. 3. was also a native of New England. 24 years old. and weighed about 60 kilogram- J. F. S.. a chemist, was a native of Canada. 29 years old. and weighed about 65 kilograms. Rest and work experiments. — In the rest experiments the subjects were as quiet a- they well could be. Practically their only muscular exercise was that involved in dressing and undressing, folding and 124 unfolding the bed. chair, and table, eating, caring for the excretory products, and weighing themselves and the absorbers. They did more or less reading and writing to pass the time. In the work experiments they spent a number of hours, generally 8 each day. driving a stationary bicycle.* It was their purpose to do a reasonable but not excessive amount of work. Experiments Nos. 1 and 2 with E. O., No. 3 with O. F. T., and Nos. 4a and 4b with A. W. S., were rest experiments, while No. 4c with A. W. S. was a work experiment. In none of these experiments were satisfactory determinations made of the outgo of energy. The experi- mental days began at different hours, and were not divided into 6-hour experimental periods, as was done in all experiments after No. 4c. Taking into account the 19 experiments, summarized in the following tables, in which the measurements of the outgo of energy were considered satisfactory, there were 9 rest experiments with E. O., covering 33 days; 1 rest experiment with A. W. S., covering 3 days, and 3 rest experiments with J. F. S., covering 9 days, making altogether 13 rest experiments, covering 45 days. There were also 2 work experiments with E. O., covering 8 days, and 4 work experi- ments with J. F. S., covering 12 days, making together 6 work experiments, covering 20 days. Altogether the 19 rest and work experiments covered a total period of 65 days. In addition, 9 rest experiments covering 22 days and 2 work experiments covering 6 days were completed during this time in connection with a separate investigation. Division of experimental days into periods. — Each of these experi- ments is divided into da} T s of 24 hours, beginning and ending at 7 a. m. Each day is divided into 4 periods of 6 hours each; the first 2, called day periods, extending from 7 a. m. to 1 p. m. and from 1 p. m to 7 p. m. ; the last 2, called night periods, extending from 7 p. m. to 1 a. m. and from 1 a. m. to 7 a. m. The chief reason for beginning the experimental day in the morning is found in the belief that the condition of the bod^v in respect to the amounts of material in the alimentaiw canal and of carbohydrates (glyco- gen) andoxvgen in "the tissues and fluids would be more nearly the same, from day to day, at the end of the period of rest and sleep than at any other time. !l Excepting in experiment ISo. 4b, in which the subject raised and lowered a weight attached to a rope which passed over a pulley. 125 SUMMARY OF RESULTS OF INDIVIDUAL EXPERIMENTS. In Table 143. below, are summarized the results of the experiments made with the respiration calorimeter which have been thus far reported by this Department. a The table shows the balance of income and outgo of nitrogen, carbon, and energy, as well as the computed gain or loss of body protein and fat. 'During the interval between the first and last of the experiments here reported nine experiments were carried on under the auspices of the Committee of Fifty for the Investigation of the Drink Problem in connection with an independent investi- gation concerning the effect of alcohol in the diet. For convenience in keeping labo- ratory records all the experiments were numbered consecutively. These experi- ments, in which alcohol formed a part of the diet, comprised Xos. 7, 10, 12, 15-20, 22, 27, 30, and 33. The details of the first two were published in U. S. Dept. Alt.. Office of Experiment Stations Bui. 69; those of the remaining eleven will be found in the Memoirs of the National Academy of Sciences. Vol. VIII, Sixth Memoir, entitled An Experimental Inquiry into the Nutritive Value of Alcohol, 1902. 126 I . © 1 (i.iiiii) pu pin; .itnonui ).>u od3M)dq .>>uruaui(i — -.; t i — i - s ~. / — r- H o fc "X — • — -.-. ri — oi ri r- ~: R, | ; i i + + + + i CO — 01 + + , co o it 04 © © oi o -r x 2 ■; © -r i- co — co -: — :: §f 7 i i + + + + i + i 1- ut 01 01 oi -r + + •oSjno it 2,879 2,894 2,287 a m h eo of of ri Ol' g of RG ri ri ~J -■ ~ ■" r'i ri n ri ri ri ri oi •araoouj i-m !paz}p -JX0 A[ll!IU»! IIUJOIVUI jo 8* | -3 i of of ef 1^ « (N H i - £ — : : Tl 01 — |H ri pi oi ri © r i ri x o ■-. / n ri ri ri •J, 260 2,804 2,242 2,048 •(-) SS0[ jo ( + ) un?S '}bj Apoq jo - 73 -135 + 171 +199 ■ 258 229 — + 1-1 cc S '- + + 218 284 885 +233 •(-)ssoi JO ( + ) un:r; •ui-uu.nl Apoq JO — o I- ri — - I I I r- © © OJ l~ r- CO I I + I ouun jo 3 :: 3 :; S £ ^L:A ■J JO c y. -r i~ — — ri ri X — r- — ^ i ~ n r. n r- ri - - 6 ~ §13| •poo j io , lCr1l^l^n©CO©r1r1 o ■_• it -r © n o © — — — x >g Jg CO ^ X I- 1- '0 irt .0 © © 7 £ 3 I- O) 00 T Cj t- oi of oi ri oi oi ri oi co of ri ri ri ri a '.-. :z ri ri xa © i^ -r ■ ( — ) sso[ jo ( + ) ureS 'ji? j I I + •(-) sso{ jo ( + ) uitiS 'uia^ojj 04 © © © © t- — ^ 1 - I I II I ri ri — ri — .r — I I + .- © © X X 1^ r- •(-) ssoj jo ( + ) un?o « i 7 + + + + + + + + ri ci cr — + ■sjonpoad Xjojxuidsaj ui .i^iointooiNM^roa pi rM ~f — DO — ' io' I- ©' © CO* .t-CCnOlOli-iOOr-ieOn COIOJOJOIOICIOJOIOIOI 219.9 217.4 216.6 196. 1 © CO © •auiju ui n CO CO CO it i- M IN » ri co .o ri oi ph •S0D3J TIJ •pooj HI x i~ © tj< x m d d « h tj< CN iO © t^ ©■ © © © i-l I- © QC X © X 1-- © n CI it © T n 1^ © iO — DC MNNNINNINOICIM cc QO © © ci d fi h © © r» ■* — i d> ©' oi l^ OI © O) it © r- l~ oi ri 1 (— ) SS0[ JO ( + ) uibo I I © © n © i 7 7 © eo co oi I I + •S9D0J III •pooj III l- CC ri CC ©'©'©'©' — X / X it © © X" •nopuma t^- © co © I I + I — t^ ■ -. — iC ,-. r: © ri it ■rj« — — ■>* •ouun "I X 1- - X - © ~ r. r X X IO ■~ \a © lt> l-~ © X) it I-' it 5 o r ? "O ■ « 5 § ! g S •* : Il| i ^ - ^ x © *" - j- ~ ~ 5 So 5b § "g ? ? ~ - - ■ -. 1- X © © CO •j.K}uuni pmag 127 «fi © H 1— 1 i^. 00 © rH se CM C 1 ) © eo CM ■N CI -1 ,_( J_ + 1 1 1 + 1 + 1 1 1 1 1 © Cl © 00 "I a m + + 1 1 1 + 1 + 1 1 1 + 1 1 © I- 8 3 re CM © C) $ r5 © r 8 '.Q l r © t_; CM X CM C-l CM TI Cl CM CM CM eo eo eo eo eo co eo eo M CM CM ~1 © iO i(C © ec X l~ -.; CM CI X © oc iO © uO © © r- Ti CM CM CM eo eo eo eo eo ec eo eo CO CM CM or S ur. iC ?c' CO CM CM CO' + + + + 1 1 1 1 1 1 1 + + ■-; CM 00 © 1- CM r eo TT s © 1 1 1 + + 1 1 • 1 1 1 1 8 .- eo © eo CN — — -1 Cl o CO id © © 05 CM CM rH t^ Cl Cl in 7 ~ -- 3 © r» © CO 1-- "* ■^ irt © © CM CN CM eo eo eo eo eo CO eo eo c CM K ex ifi a ^t< I> r- X * © © © CO rH [^ oc — iO S3 r^ Cl ci CO Tf "i eo Cs 4- + + + 1 1 1 \ 1 1 + + m c 00 © © © © © CO © © — rr X) r* «s ■"* ■* © CM irr Cl iC — eo ~T co 1 1 ! 1 + + 1 I 1 1 1 1 TI CT 00 CM ■>f © t- oo eo CM © r-i © © -r - a CO eo CM CM CO CO © eo "i -1 ri rs -i + + + - 1 1 : f 1 1 1 1 t- CC >H Tf CM © © © X © ■<* © CM rH o r- SO — C S 8 3 uO % iC s 35 uO co Cl CN CM CI eo eo eo co CO CO eo C- co CM CM — ec — M '" t>- © OM cn © © CN l> CM y-t a CM CI n CM CM ,— 1 a rH ,_, ^_ CM CM 1-1 1-1 1-1 i-l i-i 1-1 1-1 1-1 1-1 *"* o t^ CO iC •* Cn eo CO rH © © CN Cl © i-H a - c a CM © © X, ao Cl r4 CM © r^ 1-1 1-1 1-1 X a t> Tj< t^ iTi rH - iC 3 l^ t^ X c CM .~ ? OS CI so CO lO — ,4 in |> £ - CM CM. CO eo eo ec eo CO CO CO Cs CM i- :- 00 © ri io co 00 eo X © C m t^ © r- 1 rH 1 1 1 1 + 1 + 1 1 1 1 1 1 1 M t~ © Tj< ID eo o © l^ l^ a -r Cs CO ID iC h- C- 52 X r- © m iO © ©' 1, ^ ^ H 1-1 1-1 CM 1 .1 i-s — a - OQ x 'S a r^ (H 5 o fl 03 5 EC X X 0) X 02 o3 oc 0) a (U 09 CO 0) 5 a, 5 a 13 03 M p In rr> -0 - 03 1 o a < >H +a 0) 02 ft c3 rH I- eo CO o 6 0) a o > o -1 d - - o CD CI 1-5 05 •^; d o C a O Z o — 2 o r» be a > Sac > 5 o> > '— J o d T3 b« - > X bt 83 > x 1 > X > o — •d p -4-2 Is O Kate per hour. Proportion of total for 24 hours. •Subject and kind of experiment. Day periods. Night periods. Aver- age for 24 hours. Day periods. Night periods. 7 a.m. to 1p.m. 1p.m. to 7 p.m. 7p.m. to 1a.m. 1a.m. to 7 a.m. 7 a.m. to 1p.m. 1p.m. to 7p.m. 7 p.m. to 1a.m. 1a.m. to 7a.m. REST EXPERIMENTS. E.4 >., 9 experiments: Gram* Gram* 739 32.7 879 43.2 Grams 33.2 42.8 Grams 31.2 42.2 36.2 30.9 33.7 32.8 28. 1 36. 3 31 fi Grams 20.4 25.6 22. 3 21.8 24.3 23. 2 21.2 23.3 22. 3 Grams 30.8 36.6 33.9 32.8 34.0 33.2 29.8 33.4 31.8 Per. ct. Per. ct. Per.ct. Per. ct. 33 803 37.9 37.4 28. 3 28. 27. 1 16.6 A. W. S.. 1 experiment: 787 38. 1 816 41.5 797 39. 8 35.9 38.2 37.1 33. 2 40 1 Average 3 29.9 27.9 24.7 17.5 J. F. S.,3 experiments: 715 33.4 801 39.7 9 762 37 fl Rfi 1 29.1 28.4 24.9 17.6 Average of 13 ex- periments WORK EXPERIMENTS. E. 0. . 2 experiments: Minimum ! — ~ I 45 j 794 37. s 37.2 35. 22.4 33.1 ; 28.6 28.1 , 26.4 16.9 1,195 69.4 1,510 : 90.2 1,316 78.4 1,080 63 4 65.0 98.2 79.5 33.9 42. 2 38.4 27.8 36.2 31.3 19.9 26.2 23.1 21.0 24.1 22.3 49.8 62.9 54.8 45.0 53.9 50.5 Average 8 J. F. S.,4 experiments: 35. 7 36. 3 17.5 10.5 ^r 1,292 81.0 79 « Average Average of 6 ex- periments 12 1,212 73.6 74.7 36.5 37.0 15.5 11.0 20 1,253 75.5 76.6 34.1 22. 6 52. 2 36.2 36.7 16.3 10.8 The elimination of carbon dioxid under conditions of rest averaged 803 grams per day, or 33.9 grams per hour, in the 33 experimental days of the 9 experiments with E. O.; 797 grams per day, or 33.2 grams per hour, in the 3 experimental days of one experiment with A. W. S., and 762 grams per day, or 31.8 grams per hour in the 9 experimental days of 3 experiments with J. F. S. The range was from 739 to 879 grams per day with E. O., from 787 to 816 with A. W. S., and from 715 to 801 with J. F. S. It will be remembered, however, that the experiments with E. O. were made at different times during two } r ears, while those with A. W. S. were in a single experiment. Those with J. F. S. were made at short intervals during the same year. In 8 experimental days of the 2 experiments in which the subject E. O. was engaged in active muscular work for 8 hours each day the output averaged 1,316 grams per day, or 51.8 grams per hour, while in 12 experimental days in which the subject J. F. S. was similarly engaged it was 1,212 grams per day, or 50.5 grams per hour. 138 During- the rest experiments, in which the subjects had as little muscular activity as possible, there was but little difference in the elimination of carbon dioxid during the two day and first night periods. In the 9 experiments with E. O. the average rate per hour during these three periods was 37.9, 37.4. and 36.2 grams, respectively. In the experiment with A. W. S. there was a slightly larger amount of carbon dioxid eliminated in the first two periods than in the third period, the rates being 39.8, 37.1, and 32.8 grams, respectively. In the 1 experiments with J. F. S. the elimination during the first three periods averaged 37. 36.1, and 31.6 grams per hour, respectively. During the second night period, when the subject was generally asleep for nearly the whole time, the output fell off with E. O. to 22.3, with A. W. S. to 23.2, and with J. F. S. to 22.3 grams per hour. Not only are the averages with the 3 men in close accord, but the range of varia- tion in the different da} T s and experiments is decidedly narrow for all. In the average of the 13 experiments the proportion given off in each of the four periods of the da} 7 is 28.6, 28.1, 26.1, and 16.9 per cent, respectively. The elimination of carbon dioxid in the two day periods of the work experiments was more than twice as large as in the two day periods of the rest experiments. With E. O. the average amounts given off were 78.4: grams in the first and 79.5 in the second day period when at work, as compared with 37.9 and 37.1 grams, respectively, when at rest. With J. F. S. the amounts averaged 73.6 and 74.7 grams in the day periods of the work experiments, as compared with 37 and 36.1 grams in the corresponding periods of the rest experiments. The elimination in the two night periods averaged with E. O. 38.1 and 23.1 grams in the work experiments, as compared with 36.2 and 22.3 grams in the rest experiments, the differences being small; with J. F. S. the amounts w x ere practically identical, being 31.3 and 22.3 grams in the work experiments, as compared with 31.6 and 22.3 grams in the rest experiments. The uniformity in the amounts of carbon dioxid given off during the second night period in all the experiments is very noticeable. Such data ma} T perhaps be taken as an approximate measure of the metabolism of carbon in the body of an active healthy man when at its lowest ordinaiy ebb. ELIMINATION OF ENERGY. Measurements of energy. — The kinetic energy given off by the body is measured in these experiments as the sum of three quantities: (1) The heat taken up by the water current in its passage through the cham- ber; (2) the latent heat of the water vapor given off by the body — i. e., of the water vaporized by its heat and carried out in the air current, 139 due corrections being- made for water condensed upon the absorbers; and (3) the heat equivalent of the muscular work done. In the measurements of energy of income and outgo of the body the temperature of the interior of the chamber, generally about '20 degrees, is taken as the basis for computations of the heat removed or given off by food, drink, and excretory products in the chamber. a (1) The heat carried away by the water current includes (a) the heat given off from the skin by radiation and conduction: (b) that brought out of the body in the feces and urine and given off in the cooling of these excretory products to the temperature of the chamber: (e) that brought out of the body in the air. carbon dioxid. and water excreted by the lungs and skin and given off in their cooling to the same tem- perature: ('/) the latent heat of vaporization of so much of the water of c as is permanently condensed on the absorbers (mainly collected as drip water); and (e) the heat produced by the transformation of the external muscular work. The heat of . <\ and e finds its way by radiation and conduction to the surface of the copper absorbers and passes with that of d into the water current by which it is carried out of the chamber. (2) Although the air current enters and leaves the chamber at the same temperature, it carries out more heat than it brings in. The extra heat carried out is the latent heat of the water vapor added to the air of the chamber by the subject. 13 The amount of this heat is learned from the amount of water vapor and its latent heat of vaporization at the given temperature. (3) The external muscular work is measured and the heat equivalent calculated. Before leaving the chamber it is transformed into heat, which is carried away by the water current as above stated. Energy g iven off in different ways as heat and as external muscular work. — The average amounts of energy given off by the body per day in the different ways are shown in Table 118. The figures in the second column are obtained by subtracting the sum of the quantities of heat given off by the feces and urine in cool- ing and by the water in condensing on the absorbers and the heat equivalent of the external work (b-\-d-\-e above) from the total heat taken from the chamber by the water current. Accordingly they rep- resent the sum of quantities of heat given off by the skin directly and by the product of respiration and perspiration in cooling to the tem- perature of the room (a -f- c above). It is hoped that it will be possible later to make at least approximate estimates of the latter quantity and thus show the amount of heat given off by the skin alone. a U. S. Dept. Agr., Office of Experiment Stations Bui. 69, p. 20. b The differences in specific heat of the air due to loss of oxygen and gain of carbon dioxid are here assumed to be negligible. 140 Table 148. — Energy given off by the body in different ways — Amounts per day. Subject and kind of experiment. Days covered by experi- ments - duction. Heat eliminated. Heat By radia- tion and In urine and feces. lungs. Total. REST EXPERIMENTS. £. O., 9 experiments: Minimum Maximum Average A. W. S., 1 experiment: Minimum Maximum Average J. F. S., 3 experiments: Minimum Maximum Average Average of 13 experiments. WORK EXPERIMENTS. E. O., 2 experiments: Minimum Maximum Average J. F. S., 4 experiments: Minimum Maximum Average Average of 6 experiments . 33 Calories. 1,479 1,835 1,675 1,710 1,782 1,739 1,564 1,759 1,622 12 20 Calories. 19 56 33 30 34 31 17 27 23 1,669 2. 045 2,521 2, 249 2,185 2,400 2,296 2, 277 Calories. 412 715 570 486 531 509 468 520 491 Calories. 31 550 1,041 1,560 1,332 1,240 988 1,126 Calories. 2,062 2,452 2, 278 2,226 2,348 2. 279 2, 065 2, 297 2,136 193 270 228 127 277 238 234 2, 250 3.473 4,287 3,829 3, 253 3,890 3, 540 3. 656 The figures for the heat given from the urine and feces, as shown in the third column in Table 148, are calculated from the weights of these excreta, their fall in temperature, and their specific heats. The weights are found by the balance. The fall in temperature is the dif- ference between the temperature of the body and that of the interior of the chamber. This difference averages about 17 degrees. The specific heats are arbitrarily assumed, that of feces being taken as 0.9 and of urine 1.0. The figures in the fourth column represent the latent heat of vapor- ization of the water given off by the lungs and skin. For the rest experiments this water is in general that carried out of the chamber in the air current in excess of that brought into the chamber by the same current. In a few rest experiments, however, and in all the work experiments more or less water is condensed on the absorbers and is not carried out by the air current. The figures in the third column include the heat given off in the condensation of this water vapor upon the absorbers, along with the latent heat of vaporization of the water in the air current. The reason why little or no water was condensed on the surfaces of the absorbers in the rest experiments is 141 that the temperature of the incoming water current was as a rule above the dew point of the air inside the chamber. The fifth column shows the heat equivalent of the external muscular work done in the work experiments. It is measured by the bicycle- dynamo apparatus by which the mechanical work is transformed into electrical energy and into heat. According- to the figures of Table 148, in the 45 days of the 13 rest experiments the average amounts of heat given off per day from the skin (together with that in the expired air) was 1,669, in urine and feces 31, and in the water evaporated from the lungs and skin 550 calories, making the whole daily elimination 2,250 calories. With E. O. the range in total heat eliminated was from 2,062 to 2,452 and the average 2,278 calories. With A. W. S. the range was from 2,226 to 2,348 and the avereage 2,279 calories. With J. F. S. the range was from 2,065 to 2,297 and the average 2,136 calories. Taking into account the experiments with all the subjects, the average amounts of heat given off in different ways may be expressed in percentages as follows: Table 149. — Percentages of total energy given off from the body in different icays. Heat. In rest experi- ments. In work experi- ments. From skin by radiation and conduction (and in exhaled air) In urine and feces In water vaporized from lungs and skin Heat equivalent of external muscular work done Per cent. 74.2 1.4 24.4 Per cent. 62.3 .5 30.8 6.4 Total. 100.0 100.0 Energy given off from the body in different periods of the day. — Table 150 summarizes the data for the outgo of energy during the different periods of the day in the 13 experiments covering 45 experimental days. As in the two previous tables, the figures for heat eliminated include (1) the quantity given off from the body and measured by the calorimeter; (2) that given off in the water vaporized during the same periods — i. e., carried awa} T from the bod} T in water vapor; and (3) the heat equivalent of the external muscular work done. The temperature of the body and the amount of material it contains varies somewhat from time to time. a It is assumed that at the hour when the experi- mental day begins and ends — 7 a. m. — the} T will be very nearly the same from day to day. If they are the same at these times, the total quantit} T of heat in the bod}^ at the beginning and end of each experi- mental day will be the same. The total quantity of energy given off a In order to obtain more accurate data regarding variations of body temperature and corresponding changes in the store of energy in the body from hour to hour, special apparatus lias been devised for measuring the temperature of the body. See Arch. Physiol.. [Pfliiger], 88 (1901), No. 9-10, p. 492. 142 from the body during the day will in this case be equivalent to the total amount liberated within the body and will be closely parallel with the amounts of carbon and hydrogen oxidized. Taking each of these four periods of the day by itself, the differences between the amounts of heat stored in the body at the beginning and end will probably be larger than is the case for the whole day. For individual periods, therefore, the parallelism between the amounts of carbon oxidized and heat given off will hardly be as close as for the whole day. 'Table 150. — Heat given off by body, including for the work experiments the heat equiva- lent of the external muscular work — Amounts per day and rates and proportions for different periods. >> a Rate per hour. Proportion of total for 24 Days covered experimen Total anioun 24 hours. Day periods. M . , . . , Subject and kind of experiment. Aver- Day periods. Night periods. 7 a. m. 1 p. m. to to 1 p. m. 7 p. m. 7 p. m. to 1 a. m. 1 a. m. to 7 a. m. age for 24 hours. 7 a. m. 1 p. m. 7 p. m. 1 a. m. to to to to 1 p. m. 7 p. m. 1 a. m. 7 a. m. REST EXPERIMENTS. E. 0., 9 experiments: Calo- rzzz 2, 062 2, 452 2. 278 2,226 2, 348 2. 279 Calo- ries. 94.5 116.6 103. 7 111.6 119.8 114. 5 104.8 119.9 109.0 Calo- ries. 90.4 124.0 105.0 101.6 106.9 104.5 93.5 103.6 98.7 Calo- ries. 83.9 121.8 101.8 91.9 94.3 93.3 80.0 98.7 88.2 Calo- ries. 62.8 77.8 69.2 62.7 70.4 67.5 58.1 64.4 60.2 Calo- ries. 85.9 102. 2 95.0 Per cent. Per cent. Per cent. Per cent. 33 27.3 27.7 26.8 18.2 A. W. S., 1 experiment: 92.8 97.8 3 95.0 86.0 95.7 89.0 30.1 27.5 24.6 17.8 J. F. S., 3 experiments: 2, 065 2, 297 9 2.136 30.6 27.7 24. S 16. 9 Average of 13 ex- periments 45 2,250 105.5 103.7 98.5 ' 67.3 93.8 28.1 27.7 26.3 17.9 WORK EXPERIMENTS. E. 0., 2 experiments: 3,473 4,287 3,829 3,253 195.3 240. 5 212.2 192.8 281.8 231.5 108.0 128.8 120. 5 89.9 111.0 97.2 68.8 79.6 74.0 60.1 144.7 178.6 160.0 135.5 8 35.2. 36.3 18.9 11.6 J. F. S,, 4 experiments: 187.8 185.5 239. 9 '>41 . 3 3,890 70. 162. 1 64.2 147.5 12 20 3,540 212.0 216.7 35.9 36.7 16.5 10.9 Average of 6 ex- periments 3,050 212.0 222.5 100.5 08.2 152.3 34.8 1 36.5 17.5 11.2 From the quantities of heat given off from the body in the different periods of the day, as summarized in Table 150, it will be noted that in the 15 days of the 13 rest experiments with E. O., A. W. S., and J. F. S. the average amounts of heat given off per hour in the 1 suc- cessive 6-hour periods from 7 a. m. to 7. a. m. were 105.5, 103.7, 98.5, and 67.3 calories, respectively, the average hourly rate for the whole day being 93.8. The percentages of the whole amount for the day, 143 given off in the several periods, are 28.1, 27. 7^ 26.3, and 17.9, respectively. The average hourly rate for the 20 days of the 6 work experi- ments with E. O. and J. F. S. was 152.8 calories. The rates for the different periods, beginning with 7 a. m.. were 212. 222.5. 106.5, and 68.2 calories, respectively. The corresponding percentages are 34.8, 36.5, 17.5. and 11.2. It thus appears that in the two day periods in which the subjects were engaged in active muscular exercise for 8 hours the quantity of heat given off was more than twice as much as in the corresponding periods of the rest experiments. In the first night period the amount per hour in the work experiments was not greatly in excess of that in the rest experiments (106.5 as compared with 98.5 calories). In the second night period the quantities were nearly the same (68.2 in the work experiments and 67.3 calories in the rest experiments). The uniformity in the amounts of heat given off during the second night period calls for special notice. In the rest experiments with E. O.. covering 33 days, the range is from 62.8 to 77.8 and the aver- age 69.2 calories per hour. In the experiment with A. W. S.. covering 3 days, the range per hour is from 62.7 to 70.1 and the average 67.5 calories. In the 12 days of the work experiments with E. O. the amounts are a little larger than in the rest experiments with the same subject, ranging from 68. 8 to 79.6 and averaging 74 calories per hour. That the elimination of heat during this period is larger in the work than in the rest experiments is perhaps explained by the fact that while the subject was decidedly tired at night, and supposed that he slept more soundly than in the rest experiments, he moved more, and to the observers outside the chamber he appeared more rest- less. In the work experiments with J. F. S. also the amounts dur- ing this period are slightly larger than in the rest experiments, rang- ing from 60.1 to 70 and averaging 64.2 calories per hour. It is noticeable that the rate in the second night period is smaller with J. F. S: than with E. O.. the ratio of the former to the latter being about the same in both work and rest experiments From the results obtained in the second night periods in these exper- iments it would seem that from 65 to 70 calories per hour might be not far from representing an average elimination of heat by a healthy, active man in the time of minimum bodily activity. Relation between dif/iination of heat ami of carbon dioxid. — The rates of elimination of heat and of carbon dioxid in the different periods are nearly parallel, as will be seen by comparing the figures of Tables 147 and 150. Both these values in the two day periods of the work experiments are largely in excess of the similar values in the same periods of the rest experiments. The values for both thesr factors in the second night periods of the same experiments, which was one of quiet sleep, were small and noticeably uniform. 144 INCOME AND OUTGO OF ENERGY. Perhaps the most interesting results of these experiments, both because of their novelty and because of their bearing upon the metab- olism and the conservation of energy in the living organism, are those which compare the amounts of potential energy in the materials actually oxidized in the body with the amounts of kinetic energy given off from the body, either as heat alone in the rest experiments or as heat and external muscular work in the work experiments. In the rest experi- ments there was no considerable amount of external muscular work. , The little that was done would naturally be converted into heat — as. for instance, in the impact of the foot upon the floor in stepping, or of the body upon the chair or bed in sitting or lying down. The heat thus imparted to the floor, chair, or bed would naturally find its way to the absorbers, and would thus be carried out with the heat given off as such by the body. Roughly speaking, we may say that all the poten- tial energy made kinetic in the body by the oxidation of food and body material left the body as heat. This is shown by the agreement of the amount of heat given off from the body with the heat of oxidation of the material oxidized in the body. These data are summarized in Table 151. Table 151. — Comparison of income and outgo of energy in 19 experiments covering 65 expt rimental days — Amounts per day. Subjects and kinds of experiments. Xet income berof ^P° tentia ! expert energy ot - material oxidized in body). Xum- mental days. Net outgo (kinetic en- ergy given off from bodvi. Difference i in terms of net incomei. REST EXPERIMENTS. Experiments with E. O.: Experiment in which the net outgo falls farthest below the net income (Xo. 5): average for the whole experiment Experiment in which the net outgo is far- thest above the net income (Xo. 14 i: aver- age for the whole experiment Average for 9 experiments Experiments with A. W. S. : Average for 1 experi- ment Experiments with J. F. 8.: Average for 3 experi- ments Experiments with E. O.. A. W. S.. and J. F. 8.: Average for 13 experiments WORK EXPERIMENTS. Experiments with E.O.: Average for 2 experi- ments Experiments with J. F. 8.: Average for 4 experi- ments Experiments with E. O. and J. F. 8. : Average for 6 experiments Rest and work experiments: Average for 19 experiments Calories. 2,482 2, 131 2,288 2.304 2. 118 2,255 3,865 3, 539 3,669 2, mo Calories. 2. 379 Calories. -103 2. 193 2, 278 - 10 2, 279 - 25 2. 136 - 1- 2. 2.50 3,540 3, 656 2,682 - 36 - 1 - 13 - 8 -4.1 .4 ■1.1 • .8 .2 - .4 - .3 145 The figures for income and outgo* of energy require a word of explanation. A distinction is here ma*de between the total a income, which is represented by the potential energy of the food, and the net a income, which is the energy of the material actually oxidized in the body. This energy of net income is represented by the available energy of the nutrients of the food (i. e.. potential energy of total food less that of the urine and feces) minus the potential energy of the material gained or plus that of material lost by the body when the latter is not in nitrogen and carbon equilibrium. The total energy of outgo would be the kinetic energy given off from the body in heat and external muscular work plus the potential energy of the unoxi- dized materials in the urine and the feces. The net energy of outgo consists of the heat given off and the external muscular work done. The balance of income and outgo is best shown by the net rather than the total quantities. These ma}' be seen in the table given above (Table 151). The averages for the groups of experiments are for the number of days covered by the whole group — i. e., they are not aver- ages for individual experiments. It is to be remembered that the figures for net income of energy represent the heat of combustion of the material actually oxidized. This material consists mainly of the available portion of the food of which the amount and heat of combustion are found by direct deter- mination. To its heat of combustion is added that of the material lost, or from it is subtracted that of the material gained by the body. The amounts of material o-ained or lost are determined from the o-ain © © or loss of nitrogen and carbon, and their heats of combustion are cal- culated by the use of factors based upon direct determinations of the heats of combustion of similar substances. The fimires for net outo-o © © are the results of direct experimental measurement. In other words, the net income of energy is mainly and the net outgo entirely the result of direct determinations. A common usage is followed in applying the term "potential energy** to the energy latent in the food and body material oxidized. Whether chemical energy would or would not be a more correct expres- sion no attempt is here made to decide. Ordinary usage is also fol- lowed in expressing potential energy in terms of heat— i. e.. as cal- ories — thus employing a unit of kinetic energy for the measure. This discrepancy is unavoidable, since we have neither the means for meas- uring potential energy as such, nor a unit for expressing such measure- ments if they were made. The use of heat of oxidation for the meas- ure is especially appropriate here, since the energy is liberated mainly by oxidation and appears chiefly or entirely as heat. a The terms "total" and "net" here applied to income and outgo of material and energy are used for present convenience, and may in future reports be replaced by more satisfactory expressions. 13007— No. 109—02 10 146 The conservation of energy in'the body. —If the law of the conserva- tion of energy obtains in the living organism, the net income and the net outgo of energy should be the same. In such physiological experi- menting, however, it would be hardly fair to expect the figures for the two to agree for each day of a given experiment or for each experiment as a whole, even if the measurements with the respiration and bomb calorimeters are exact. There may be errors in the estimates of the amounts and heats of combustion of the materials actually oxidized. Variations due to irregularities of the physiological processes of the body are unavoidable and may materially affect the results. For instance, the calculations assume that the quantities of material in the alimentary canal and of carbohydrates in the body as a whole are the same at the end as at the beginning of each day or experiment, whereas they may differ considerably, and the differences would materially affect the results. But it might be hoped that, if the methods are correct, these errors would tend to counterbalance one another in a series of experiments, and that, in the average of a sufficiently large number, the errors would thus be eliminated, so that the income and outgo would be very nearly the same. Exactly this is the case in the data here reported. The variations for individual days, and even those for the individual experiments, as shown in the detailed tables in this and the previous bulletins, are not inconsiderable, but in the average of all the experiments the agree- ment is very close. Thus, in the 33 days of the 9 rest experiments with E. O. , according to the figures for the individual days the net outgo varies from 165 calories below to 194 calories above the net income. Expressed in percentages of net income, the range here is from —6.5 to +9.1 per cent. Both these extremes occurred on the first days of the respective experiments. In general, the results for the first day of an experiment are found to be less satisfactory than those for the succeeding da3 T s. Considering each experiment as a whole, and comparing the averages of the several experiments one with another, the range of variation is less. Here the net outgo varies from 103 calories below to 62 calories above the net outgo per day. Expressed in percentages of net income, the range is from —4.1 to +2.9 per cent. But in the average for the 9 experiments the figures for the net income and outgo are practically the same, being 2,288 and 2,278 calories, respectively. In the average of the 2 da}^s of the rest experiment with A. W. S. there is a disparity of 1.1 per cent, and in the average of the 9 days of the 3 rest experiments with J. F. S. the difference is 0.9 per cent. Taking the 45 days of the 13 rest experi- ments together, the average income is 2,255 and the average outgo 2,250 calories; the difference is 0.2 per cent. In the average of the 8 days of the 2 work experiments with E. O. the net income and outgo are 3,865 and 3,829 calories, respectively, the difference being 147 0.9 per cent, and in the average of the 12 days of the 4 work experi- ments with J! F. S. the net income and outgo are 3,547 and 3.540 calories, or a difference of 0.2 per cent. Taking into account the 6 work experiments with E. O. and J. F. S., the net income is 0.5 per cent larger than the net outgo. The averages for the 65 days of the 19 experiments are: Income, 2,691; outgo. 2.682 calories. The dif- ference, 0.3 per cent, is far within the limits of experimental error and physiological uncertainty. In experiments of this kind, which represent only the work of a period during which experience with new apparatus and methods is being accumulated, individual discrepancies such as those above recorded seem no larger than might naturally be expected. The agreement of the average results is much closer than was hoped for, and we regard it as by no means certain that future averages will show so exact a balance. At the same time it is to be noted that when, to the results of the 19 experiments here summarized, those of the 11 others above referred to as published elsewhere are added, the agreement is almost absolute. The 30 experiments covered, all told, 93 days. The average daily income was 2,719 calories and the outgo 2.716 calories. It thus appears that, with increase in the number of experiments, the differ- ences due to unavoidable errors more and more nearly counterbalance one another. The general subject of metabolism of energy in the living organism will be more profitably discussed when data not yet published are available. Meanwhile it is safe to say that the results thus far obtained fall very little short of definite demonstration of the action of the law of the conservation of energy in the living organism. O LIST OF PUBLICATIONS OF THE OFFICE OF EXPERIMENT STATIONS ON THE FOOD AND NUTRITION OF MAN-Continued. Bur. 67. Studies on Bread and Bread Making By Harry Snyder and L. A. Voorhees. Pp. 51. Price. 10 cents. Bui. 68. A Description of Some Chine-e Vegetable Food Materials and Their Nutri- tive and Kconnniic Value. By \V. f ('. Blasdale. Pp. 48: Price, 10 cents. Bui. 69. Experiments on the Metabolism of Matter and Energy in the Human Body. By W. 0. Atwater and F. < r. Benedict, with the cooperation of A. W. Smith and A. P. Bryant. Pp. \\% Price. 10 cents. Bnl. 71. Dietary Studios of Negroes in Eastern Virginia in 189/ and 1S98. By H. B. Frissoll and Isabel Bevier. Pp. 45. Price, 5 cents. Bui. 75. Dietary Studies of University Boat Crews. By W. O. Atwater and A. P. Bryant. Pp. 72. Price, 5 cents. Buh 84. Nutrition Investigations at the California Agricultural Experiment Station, 1896-1898. By M. E. Jaffa. Pp. 39. Price. 5 cent-. Bnl. 85. A Report of Investigations on the Digestibility and Nutritive Value of Bread. By Chas. D. Woods and E. II. Merrill." Pp. 51. Price. 5 cents. Bui. 89. Experiments on the Effect of Muscular Work upon the Digestibility of Food and the Metabolism of Nitrogen. Conducted at the Univorsitv of Tennessee. 1897-1899: By 0, E. Wait. Pp. 77. Price, 5 cents. Bui. 91. Nutrition Investigations at the Univorsitv of Illinois, North Dakota Agri- cultural College, and Lake Erie College, Ohio, 1896-1900. By H. S. Grindley and J. E. Sammis, E. F. Ladd, and Isabel Bevier and Elizabeth C. Sprague. Pp. 42. Price, 5 cents. Bui. 98. The Effect of Severe and Prolonged Muscular Work on Food Consumption, Digestion, and Metabolism, by W. O. Atwater and H. C. Sherman, and The Mechanical Work and Efficiency of Bicyclers, by R. C. Carpenter. Pp. 67. Price, 5 cents. Bui. 101. Studios on Broad and Bread Making at the Universitv of Minnesota in 1899 and 1900. Bv Harry Snvder. Pp. 65. Price. 5 cents. Bui. 102. Losses -in Cooking Meat. 1898-1900. By H. S. Grindley, H. McCormack, and H. C. Porter. Pp. 64. Price, 5 cents. Bui. 107. Nutrition Investigations Among Fruitarians and Chinese at the California Agricultural Experiment Station, 1899-1901. By M. E. Jaffa. Pp. 43. Price, 5 cents. farmers' bulletins. Fo*ods: Nutritive Value and Cost. By W. O. Atwater. Pp. 32. Meats: Composition and Cooking. Bv C. D. Woods. Pp. 29. Milk as Food. Pp. 39. Fish as Food. Bv C. F. Langworthv. Pp. 30. Sugar as Food. By Mary H. 'Abel. ' Pp. 27. Bread and the Principles of Bread Making. By Helen W. Atwater. Pp. 38. Beans, Peas, and other Legumes as Food. By Mary H. Abel. Pp. 32. Eggs and Their Uses as Food. By C. F. Langworthv. Pp. 32. Principles of Nutrition and Nutritive Value of Food. CIRCULAR. Cir. 46. Foods for Man. By C. F. Langworthy. Pp. 10. SEPARATES. Food and Diet. Bv W. (). Atwater. Reprinted from Yearbook of Department of Agriculture for 1804. Pp. 44. Some Results of Dietary Studies in the United States By A. P. Bryant, Reprinted from Yearbook of Department of Agriculture for 1898. Pp. 14. Development of the Nutrition Investigations of the Department of Agriculture. By A. C. True and R. D. Milner. Reprinted from Yearbook of Department of Agriculture ior 1899. Pp. 16. The Value of Potatoes as Food. By C. F. Langworthy. Reprinted from Yearbook of Department of Agriculture for 1900. Pp. 16. *Bu 1. 23. Bui. 34. Bui. 74. Bui. 85. Bui. 93. Bui. 112. Bui. 121. Bui. 128, Bui. 142. ■illfc;