State of Contiecttcut REPORT OF The Connecticut Agricultural Experiment Station NEW HAVEN, CONN. INHERITANCE IN CORN BEING PART VI OF THE ANNUAL REPORT OF 1911 JUNE, 1912 CONCLUSION ilNi*^^ ih. . it9 PART VL INHERITANCE IN CORN. By H. K. HAYES. Introduction For the last six years this Station has been studying the inheri- tance of corn characters, and in 191 1 a technical bulletin was published on the subject. The purpose of this paper is to state as clearly as possible some of our results which have practical value to corn growers and breeders in two different particulars. First, a large number of corn varieties are grown in Con- necticut and often several of them on the same farm. In spite of usual precautions, slight accidental crossings between varieties take place which cause serious embarrassment to growers of seed corn and plague everyone who wants to raise corn which is uniform in appearance. In the following pages is stated what can be done to detect and cull out the accidental hybrid seed. Second, many more or less successful attempts have been and are being made to develop new hybrid varieties which shall com- bine desirable qualities from both parents. Accurate knowledge of the way in which these desirable qualities are inherited will simplify operations and prevent disappointment. The Formation of the Seed Following the general rule, seed corn can only be produced by a union of male and female elements. The tassels and silks of corn, which are the male and female reproductive organs, are borne on widely separated parts of the plant. Each thread of silk grows from a spot on the cob where the kernel is to be and where the female cells are produced. The office of the silk is to collect the pollen grains which are formed in the tassel. A mature pollen grain falling on a mature silk germinates and sends out a tube which, guided by the silk, reaches the ovary. Through this tube two male cells pass, and on reaching the female cells, 4o8 CONNECTICUT EXPERIMENT STATION REPORT, IQII. unite with them. One unites with the egg cell to form the embryo or undeveloped plant and the other fuses with a second female cell to form the endosperm of the seed. This endosperm is the surrounding tissue in which the food material is stored. The embryo of the corn seed is the germ or chit, the rest of the seed within the outer covering is the endosperm. The fusion of the cells just described, called fertilization, is at once followed by rapid growth and the production of a seed. When the pollen comes from the same plant which bears the silk, there is "self-fertilization ;" when it comes from another plant of the same variety there is "cross-fertilization;" when from another variety there results "hybridization." As a commercial variety of corn is generally composed of many types in a complex hybrid condition, due to constant inter-crossing, there is no exact distinction between "cross-fertilization" and "hybridization" as applied to corn. The Law of Heredity The transmission from one generation to another of the promi- nent features, such as sweet, dent and flint characters, color of seeds, etc., which distinguish varieties of corn, follows what is known as Mendel's Law of Heredity, which may be illustrated from his own work with peas. For this discussion Darbishire's book, "Breeding and the Mendelian Discovery," has been freely used. The edible pea may be divided into two classes, the tall and the dwarf. One difference between them is in the length of the internodes, i. e., the sections of the stem between two nodes or joints where the leaves are attached. This causes the tallness or the shortness of the whole plant. Moreover, the pea is a normally self-fertilized plant, i. e., seed is formed by the union of male and female cells of the same plant. This seed, if from a tall race, will produce nothing but tall plants, and if from a dwarf plant will yield dwarfs only. Now, if a cross is made between a tall and a dwarf race by applying the pollen of one variety to the stigma or female recep- tive organ of another variety, and the seeds produced by this "cross" are sown, nothing but tall plants will appear. These are no shorter than the pure tall plants and in many cases they are THE LAW OF HEREDITY. 409 somewhat taller because of the increased vigor due to crossing two pure races. When we sow the seed from the above hybrid generation we obtain tall and dwarf plants in the ratio of about three tall to one dwarf in every four. These dwarfs, if self-fertilized, will all breed true as to height in later generations, but while some of the tall plants will breed true others will again give tall and dwarf plants in the ratio of three to one. These facts are easily explained by the present Mendelian theory, which is not essentially different from Mendel's inter- pretation. A plant or animal does not transmit its characters in a bunch as it were, as if the entire organism were the unit, but its various characters are inherited separately. Each reproductive cell, whether in pollen or ovule of the tall pea, contains a factor, structure or unit quality of tallness which we may represent by T. A union of male and female cells of tall peas will be a union of reproductive cells, all of which contain T, and a tall race will result. Each such reproductive cell of the dwarf pea lacks the structure or factor which produces the tall race. This lack may be represented by t, and from their fusion only dwarfs result. But when the tall and dwarf races are crossed, each seed is the result of a union of reproductive cells, the one from the tall race containing T and the one from the dwarf race containing t. But the tall character in this generation is "dominant;" i. e., it conceals or masks the other. Such characters as tallness and dwarfness are said to be contrasted or allelomorphic pairs, and as only tall plants are produced when tall and dwarf are crossed, the tall character is said to be a dominant one and the dwarf character a recessive condition. Now, if all the seeds of this hybrid are planted and their blos- soms self-fertilized, we may explain the conditions as follows : Half of the male cells are supposed to contain only T, the factor for tallness, and half only t, the factor for dwarfness. The same is true of the female cells. We may represent the situation and the resulting progeny thus : T- -^T Male T^ > t 41 CONNECTICUT EXPERIMENT STATION REPORT, I9II. From the diagram it appears that one-quarter of the resulting seeds have received T from both pollen and ovule, or (T + T). They can produce only tall progeny. One-quarter have received t from both, or (t -j- t) and can produce only dwarf progeny. The remaining half of the seeds are like those from which they themselves were grown, i. e., they are formed by a union of cells, one of which contains T and the other t, or (T -f t). But when the dominant factor T is received from only one reproductive cell the plant when grown is a tall one and can not be distinguished from the pure tall race. The only method of distinguishing between the pure tall and hybrid tall plants is by breeding from them. The pure tall plants will, of course, give only tall progeny, while the hybrid tall plants will again give both tall and dwarf plants. The first important fact which this illustrates is that the external appearance of a plant is not a correct criterion of its breeding qualities, but that the contents of the reproductive cells are the important feature, and that in most cases the only way of determining these contents is to breed from them. We should also note that only one of the factors of a con- trasted pair is found in a single reproductive cell. Thus, in the case above described no reproductive cell consisted of a mixture of the factors for both tall and dwarf plants but contained either the one or the other. The fact that there is often a dominance of one condition over another in the first hybrid generation of a cross is of especial importance to the corn growers of Connecticut. A partial report of this matter was given in 1907 by East. Dominance of Characters in Corn Breeding After crossing, the characters here given behave in the follow- ing manner : Flint or Dent is dominant to Sweet. Yellow " " "No Yellow or White. Purple " " " No Purple or White. Red Pericarp " " " No Red or White. Red Cob " " " No Red or White. FLINT OR DENT — SWEET CROSSES. 4II Flint or Dent — Sweet Crosses If a sweet corn is used as the female parent and is crossed with a starchy corn, either a flint or a dent, there result hybrid seeds which cannot be distinguished from seeds of the starchy parent. This result is due to the fact that the endosperm of corn is hybridized as well as the embryo. (See The Formation of the Seed.) A microscopic study of the starch grains of sweet corn shows them to be small, angular and abortive, while those of a flint or dent are much larger and are circular in outline. Something is evidently introduced by the pollen of the starchy corn which enables the sweet variety to proceed in its starch development and form seeds which are not visibly different from the starchy parent. If, on the other hand, starchy corn, flint or dent, is used as the female parent and is pollinated with sweet corn pollen, no immediate effect is apparent. The starchy corn already contains the dominant factor and masks or hides the presence of the sweet character which is recessive. The practical use of these facts is as follows : No extreme care need be used in isolating sweet com plots from field corn, as any crossing on the sweet corn shows in the first year, and at harvest the hard starchy kernels can be dis- carded. The sweet kernels, those with a wrinkled appearance, when grown v/ill always produce sweet corn. But, on the other hand, as there is no immediate visible change when field corn is pollinated with sweet corn pollen, there is no method of detecting and rejecting the hybrid kernels. The field com seed should always therefore be saved from that part of the field which is farthest from the sweet com plot. Sometimes seeds of a semi-starchy nature appear in sweet corn varieties. In a study of the inheritance of starchy-sweet crosses a few ears of this nature were met with. Semi-starchy seeds have also been mentioned as occurring in a number of pure sweet races by Halsted of New Jersey. To determine the inheritance of this peculiarity, the most starchy and most sweet kernels were selected from a self-fertilized semi-starchy ear. The result of two years' selection of the most wrinkled kernels for planting produced ears nearly all of which were of a pure sweet nature. No ears have as yet been produced of a pure starchy nature. 412 CONNECTICUT EXPERIMENT STATION REPORT, I9II, Such semi-starchy ears are often encountered by sweet corn canning factories and give much trouble. Selection of the most wriukled seeds for planting will assist in eliminating the semi- starchy types. Yellow — White Crosses Our results indicate that in many cases there are two separately inherited characters for yellow color in corn, either of which can produce this color. This fact, although of great scientific in^pprtance, does not materially change the methods of producing pure seed of either the yellow or white variety. The color which produces the yellow varieties of corn is an endosperm character. When white com is crossed with pollen from a yellow variety the resulting progeny always has a yellow color, although it is sometimes lighter than the pure yellow. If the white corn plot is not completely isolated from the yellow variety some hybrid seeds will be produced. On saving the white corn seed, those seeds which are hybrid can be detected by their yellow color and removed, with the assurance that when nothing but white seed is planted the seed is pure for this character. When yellow is the female parent and is crossed with white corn the resulting seed is yellow. In some crosses the hybrid seeds are light yellow, but, on the other hand, some hybrid yellow- white seeds are dark yellow, so it is impossible to separate the pure yellow from the hybrid yellow seed except by breeding. Therefore, yellow corn, when intended for seed, must be grown at a considerable distance from white varieties. Intentional crossing of two varieties is usually done with the idea of obtaining an improved variety by combining the desirable qualities of both parents. Suppose a white corn with a large stdlk has bfeen crossed with a yellow variety with a good ear and that a type has been produced with both these characters (large stalk and good ears), but the ears are composed of such a heterogeneous mixture of )^ellow and white seeds that they have no value as seed corn. The problem is to obtain ears which will produce either all white or all yellow seeds. The easiest method is to select only pure white seeds for planting, which in turn will produce only white corn. If a yellow variety is desired it can be most easily produced by planting all yellow seeds and self- PURPLE — WHITE CROSSES. 413 fertilizing a number of ears, i. e., the pollination of the silks of a plant by its own pollen grains. This is most easily performed as follows : Just before the silk appears a bag should be placed over the ear and another over the tassel. About five days later, when the silk is well showing, the stalk should be bent over and the tassel bag carefully removed. This will contain a certain amount of yellow dust or pollen which should be carefully dusted over the silks, and the ear again covered. After maturity, these hand- fertilized ears should be harvested and examined. All ears which contain only yellow seeds will produce only yellow progeny. It is necessary to self-fertilize a large number of ears so that several pure ears may be allowed to cross naturally the following season in order not to obtain evil effects from inbreeding. Purple — White Crosses Only a few races of purple com are grown in Connecticut, of which Black Mexican sweet is the best known example, although a purple pop variety is sometimes seen in the market. This purple color is due to a dye which is present in a single row of cells, known as the aleurone layer, which is found just under- neath the pericarp or outer hull of the seed. As this layer is a part of the endosperm, there is an immediate effect when non- purple races are crossed with purple varieties. The conditions, however, are different than in the crosses previously mentioned. From an analysis of crosses between Black Mexican sweet and several non-purple races the following facts have been established. The purple color is due to at least two separately inherited factors found in the reproductive cells, both of which must be present in order to produce a purple condition. By crossing a white race which we found to contain one of these factors with another white race which contained the other factor, purple seeds were obtained. In one cross there was no visible effect in some kernels when a non-purple race was crossed with pollen from a purple variety. This we explain by the presence of something in this race Avhich inhibited the production of purple. These facts, however, will not be further discussed here. When pure seed of a purple race is desired, it is necessary that it be isolated from other corn plots. Of course, when Black 414 CONNECTICUT EXPERIMENT STATION REPORT, I9II, Mexican sweet is fertilized with pollen from starchy races, either dent, flint, or pop, those seeds which are of a hybrid nature will be of a hard starchy condition and can therefore be rejected. Non-purple races, of either flint, dent, pop, or sweet, from which seed is saved, should not be grown near a purple variety, as there is no surety that hybrid seeds can be rejected. All kernels showing any purple color will be found to be hybrids and by rejecting these the greater part of the hybrid seeds can be removed. Red Pericarp — White Pericarp Crosses The pericarp of corn is the outer hull. It is in this portion of the seed that the red color of the common red races of corn is found. If a paper bag be placed upon an ear before silking time and be removed after the silks have fully developed, thus leaving it unfertilized, the places on the ear where the seeds would have been formed, had pollination taken place, will be found to consist almost entirely of this outer hull or pericarp. This portion of the seed is therefore just as much a part of the mother plant as its tassels or silks and is not immediately affected by pollination. For this reason an ear has either a red pericarp color on all of its seeds or on none of them. If a seed is soaked for a short time in water this outer hull may be easily removed. This red color conceals all other colors which may be present in the seed. Thus, an ear of corn may contain both yellow and white, or purple seeds, or any of these colors in a pure condition, yet if the red pericarp is present these facts are obscured. Thus we are further impressed with the fact that all of the characters so far discussed are separately inherited. If a red pericarp corn is crossed with a non-colored race, no matter which is the female parent, there will be no immediate visible effect. If these hybrid seeds are planted, the crop for the following season will consist of all red pericarp ears. If these ears are self-fertilized and grown, red and non-red pericarp ears will be received in the ratio of three to one. Besides this solid red pericarp there are mosaic red, commonly called "Bloody Butcher," varieties. This mosaic red is inherited as a pattern color. In common with many variegated races of RED COB — WHITE COB CROSSES. 415 plants this condition does not breed true but always gives some non-colored and, in some cases, some deep red progeny. There is also a pericarp color which varies in intensity due to light condi- tions. If the ear is stripped of its husks before maturity all seeds are partially covered with red, in other cases the red color is only seen as a slight blush on some seeds near the tip of the ear. This color is inherited in the same manner as the deep red pericarp color. As there is no method of detecting a cross between red and non-red races, seed of either sort should only be grown in isolated plots at some distance from the other variety. Red Cob — White Cob Crosses The directions for selecting exhibition com, issued by the extension department of the Massachusetts Agricultural College, state that white corn usually has white cobs, that red cobs in yellow corn are preferred, while a variation in cob color shows a mixture and poor breeding. It is not believed that the color of the cob is of any practical importance, although for the sake of a uniform appearance a "mixture" is not advisable. In a study of a cross between a red pericarp, red cobbed variety, and a race which lacked these colors, the pericarp and cob colors were coupled in inheritance, i. e., when separation took place all red cobs had red pericarps and all white cobs had white pericarps. In other cases no coupling has been observed and each character is separately inherited. The cob color behaves exactly similar to the pericarp color in crosses. Red is dominant to white and in the second generation there are, on the average, three red cobs to one white. In case of a mixture of red and white cobs, or pericarp colors, pure races for either the one or the other can be most quickly got by self-fertilizing a number of ears, as explained under the heading Yellow-White Crosses. All non-colored self-fertilized ears for either cob or pericarp will give non-colored progeny. Of the self fertilized red ears, one-third will breed true and two- thirds will again give a mixture. The breeding nature of these can be tested by growing about ten hills of each and noting results. The remainder of the seeds from each ear should be reserved and all ears which, by test, give only red-eared progeny can be used to m.ultiply the variety the following year. 4l6 CONNECTICUT EXPERIMENT STATION REPORT, I9II. Summary of Crosses Showing Dominance In the preceding discussion we have considered the appearance of crosses, the production of seed of pure varieties, and the inheritance of characters. Summing up these results, we find that when a white corn is crossed with a yellow variety the resulting seeds are always yellow. Likewise when sweet corn is pollinated with starchy races (dent, flint, or pop) the resulting seeds are always of a starchy appearance. Pure seeds for the characters, sweetness, or white color can therefore be told by inspection. When ears show a mixture of yellow and white or starchy and sweet seeds we may be sure that the white seeds and the sweet seeds are pure for these respective characters. Thus no extreme isola- tion from other varieties of either sweet or white corn seed plots need be practiced, as the crossed seeds can be detected and elim- inated. Seed plots in all other cases must be isolated from other varieties. In case a mixture has been received of yellow and white seeds and a yellow race is desired, this can be obtained by planting yellow seeds and self-fertilizing a number of ears, as we know that all self-fertilized ears which contain only yellow seeds will thereafter give yellow progeny. When a variety produces some colored and some non-colored ears for either cob or pericarp colors, there is no surety that either selected color will breed true. As a larger part of the seeds of an ear is normally "cross-fertilized" there is a small chance of receiving either a red or white ear in which some seeds will not be fertilized with pollen from a plant bearing an opposite color and, when planted, give a mixture. Pure varieties can be most quickly obtained by self-fertilizing a number of ears as previously explained under the heading "Red Cob-White Cob Crosses." It is always necessary to self-fertiHze a number of ears so that several pure ears may be allowed to cross naturally the following season. When attempting to produce an improved variety by crossing two types, each of which contains a desirable character, the aim of the breeder is to combine both desirable characters in one variety. As we have learned, each character is generally sep- SUMMARY OF CROSSES SHOWING DOMINANCE. 417 aratel)^ inherited, although some cases of coupHng have been reported in which two or more characters are inherited together. The method of inheritance of any character can be determined by crossing a variety which contains this character with another in which it is either absent or is present in a modified condition and by studying the appearance of this character in later generations. The knowledge of the manner in which each character is inherited enables the breeder to combine desirable features of one variety with those of another. When the parents differ by two separately inherited characters we may illustrate the results as follows : Suppose one parent is a yellow sweet corn in which there is only one inherited factor for the yellow color and the other parent is a white flint corn. As we have seen, yellow is dominant to white, and flint or starchiness is dominant to sweetness. Since these are endosperm characters, the immediate cross of the above parents will be a yellow flint corn. But the following generation, if self-fertilized, will consist of ears containing four sorts of seeds in the ratio of nine yellow starchy, three white starchy, three yellow sweet and one white sweet. The yellow starchy com is produced when the factors for yellow and starchiness are both present. As both are dominant factors, only a part of the nine yellow starchy seeds will breed true. Theoretically, one out of every nine will give ears containing only yellow starchy seeds, two will breed true for the yellow color but will be hybrid for the starchy character, two others will breed true for the starchy con- dition but will give both yellow and white seeds, and four will again produce ears containing all four sorts of seeds. Of the three white starchy seeds, all will breed true to the white color, but only one of every three will give pure sta;rchy ears. Likewise, of the three yellow sweet seeds, all will give sweet progeny, but only one out of every three will give pure yellow ears. The white sweet seeds will all breed true, giving only white sweet progeny. Our parent varieties were yellow sweet and white flint. From crossing these varieties two new sorts have therefore been pro- duced, namely, white sweet and yellow flint. This cross illus- trates what we mean bv a recombination of characters. 4l8 CONNECTICUT EXPERIMENT STATION REPORT, I9II. Inheritance of Characters Which do not Show Dominance From the viewpoint of the improvement of corn by a cross between two varieties, each of which contains some desirable character, the phenomenon of dominance is of little importance. The result desired is to obtain a race which contains both bene- ficial characters. The following crosses conform to the essential feature of Mendel's law, i. e., the separation of characters in the reproduc- tive cells of hybrids and their chance recombination — although the conditions are of a complex nature. After crossing, the characters given below do not show domi- nance. The first hybrid generation is intermediate in appearance and the second hybrid generation gives both intermediate and parent types. Crosses between Flint and Dent races. Crosses between races which differ in row numbers. Crosses between races which differ in height of plants. Crosses between races which differ in length of ears. Crosses between races which differ in size of seeds. Crosses between Flint and Dent Flint corn has hard, smooth and oval grains generally nearly as broad as long, while dent corn is indented on the top and the length of the seeds is much greater than their breadth. The characteristic difference between flint and dent is due to the amount and position of homy starch in the endosperm. Flint seeds have the embryo and soft starch surrounded by the horny starch. In dent races the soft starch extends from the center to the cap, the sides of the seed being composed of homy starch. There is no immediate visible effect when dent and flint races are crossed, the character being inherited as a plant character. For this reason seed of either sort must be produced in isolated plots. Frequently an ear from a flint-dent cross contains both indented and flinty seeds, yet selection of either kind for planting gives like results. An ear which contains both flint and dent seeds is generally a hybrid. The first generation of a cross between a flint and dent is of a uniform nature and intermediate in appearance. Sometimes this generation is more like the flint parent, sometimes more like dent CROSSES BETWEEN RACES WITH DIFFERENT ROW NUMBERS. 419 in appearance and in other cases strictly intermediate. Self- fertilized ears of this generation produce a wide range of varia- tion the following season, a few ears resembling flint, others resembling dent, and the greater part again of an intermediate nature. Self-fertilized ears either of the pure dent or pure flint type produce pure races when grown, but the intermediates again show wide variabilities. In this cross there is often difficulty in separating pure dent or pure flint from intermediates, by inspec- tion. As in some previous cases, the only sure method of deter- mining the breeding nature of an ear is by growing it and examining its progeny. Crosses between Races which Differ in Row Numbers There is a wide range of row classes in corn. The most com- mon flint races are eight-rowed sorts and some dent races produce as high as thirty-six rows. This character is quite markedly affected by conditions ; thus, an eight-rowed flint frequently produces some twelve-rowed ears. A sixteen-rowed selection which has been inbred for a number of years has a normal fluctu- ation of from twelve to twenty rows with a mean value of sixteen rows. In pure races these fluctuations, due to environmental or physiological conditions, are non-inherited. Crosses between races which differ in row numbers are of an intermediate nature in the first generation, the range of classes being somewhat larger in the second generation than in the first. Some of these second generation plants breed comparatively true, although the greater part again break up the following year. There must be several inherited factors for row classes in corn, as different varieties breed true to different row classes. The row classes of most commercial varieties of corn can be changed by selection and either increased or decreased. This is due to the fact that corn is naturally cross pollinated, and a commercial variety really consists of a complex hybrid condition. Crosses Between Races which Differ in Height of Plants Plant height is a complex character and, without doubt, due to many inherited factors. Our reasons for this belief are the large number of known varieties which differ from each other in plant height. In common with other size characters, environmental 420 CONNECTICUT EXPERIMENT STATION REPORT, I9II, conditions have the power to greatly modify the height of plants. The fluctuations within a pure race are very large and make the analysis of the inheritance of such characters almost impossible. Some of our experimental races have been constantly inbred for a period of six years and are very uniform in appearance. Under ordinary field conditions, the difference in height of dif- ferent plants of such pure races is often as great as two and a half feet. Two crosses have been studied between races which differ in plant height, both giving similar results. One was a dent-flint cross and the other a cross between Tom Thumb pop and Black Mexican sweet. The results were as follows : The first genera- tion of the cross proved to be as uniform as either parent and was somewhat larger than the average of the parents. This increased height over the average of the parents is due to increased vigor and not to dominance. Self-fertilized ears of this first generation, when grown, pro- duced a wide range of variability for plant height, embracing the range of both parent forms. Of course, it is not to be expected that many of these forms will breed true the following year, yet selection of forms like either parent will doubtless give an approach toward the parental condition. Length of Ears and Size of Seeds Only one cross between races which differ in ear length has been carefully studied. This was between Tom Thumb pop, with an ear length of from five to eight centimeters, and Black Mexican sweet, with an ear length of from thirteen to twenty-one centi- meters. The first generation of this cross had a range of varia- tion of nine to fifteen centimeters. This generation was no more variable than the parent races, and was of an intermediate ear length, although somewhat larger than the average of the parents. The second generation was very variable and produced some ears which closely approached the parental forms in length. The above cross was also used to study the inheritance of weight of seeds. The size of the seeds is determined in a large measure by the pericarp, which is a mother plant character. For this study, twenty-five seeds were weighed from each ear. The first generation was intermediate in weight of seeds and no more INHERITED DWARF FORMS. 42 1 variable than the parent forms. The second generation presented a wide range of variation. Some short ears produced large seeds, while some long ears produced small seeds. Summary of Crosses not Showing Dominance The preceding crosses do not show the phenomenon of domi- nance, yet all behave in a similar manner. The first generation of these crosses is of an intermediate appearance and the second generation has a range of variation from one parent form to the other. Some of these second generation forms will breed true, but it is impossible to determine, by inspection, which these are. The only method of determining the breeding nature of these second generation forms is by row tests. Abnormalities There are a number of abnormalities of corn which are often found in commercial races. These appear to be of two sorts ; first, those which arei produced by some unusual environmental condition and are non-inherited, and second, inherited abnormali- ties. Of these two classes the heritable one is of interest to the commercial grower or seedsman because it is important to know the quickest method of getting rid of these abnormalities if they appear. The following abnormalities have been found to be inherited: Dwarf forms. Divided ears. Irregularity of rows of seeds. Dwarf Forms Dwarfs have appeared several times in our cultures, and in all cases but one in strains which have been inbred for one or more seasons. Inbreeding, as used here, means the pollinating of the silks of a plant by its own pollen grains. In one instance, some dwarf plants appeared in a commercial culture of Stowell's Evergreen Sweet. A normal plant from this culture was inbred and gave both dwarf and normal plants the following season. A cross was made between a normal plant and a race in which dwarfs had never appeared, which, when grown, 42 2 CONNECTICUT EXPERIMENT STATION REPORT, I9II. gave only normal plants, two of which were self-fertilized. One of these ears produced only normal plants, and the other gave both normal and dwarf forms. Several attempts to self-fertilize such dwarfs have proved unsuccessful and in one case when a few mature seeds were produced they failed to germinate. Emerson of Nebraska suc- ceeded in self-fertilizing some similar dwarfs which appeared in his cultures and when grown only dwarfs were produced. It seems very probable that we are dealing with a condition in which normal plants are dominant to dwarfs, as is the case of the tall and dwarf peas. The dwarfs which have appeared in our corn cultures, unlike dwarf peas, are absolutely valueless. The low per cent, of seeds which germinate from strains which pro- duce some dwarfs points to the fact that in some cases such abnormalities have not been able to develop. If dwarfs appear in an otherwise valuable strain they can be most quickly eliminated as follows : By hand pollinating a large number of ears we may expect to receive one out of every three which will give only normal plants. The hand pollinated ears should be tested by planting a part of each and reserving the remainder of the seeds. The seed from all ears which in this test gives only normal plants may be used to develop the strain the following season. Such a method should not be used unless the strain is a valuable one. Divided Ears A desirable ear of corn should be cylindrical in form and should not have too large a cob, i. e., the proportion of shelled corn to cob should be large. There are several classes of abnormalities which show different degrees of division of the cob. A form with a monstrous, flat- tened, and in extreme cases a divided tip, is frequently seen in races which have a large number of rows, such as dents and pops. At our State Fair this year, one entry for the best six-ear lot of yellow dent corn contained one such ear. Such an ear produces a large proportion of cob which is valueless and also an ear which is hard to shell. The above abnormality belongs to the inherited class, although environment has the effect of retarding or accelerating its mani- INHERITANCE OF IRREGULAR ROWS. 423 festation. One of our inbred races of dent produces, on the average, about two-thirds of its progeny with a flattened ear and one-third with both a flattened ear and a divided tip. A cross between this strain and a strain with a flattened cob gave, in the first generation, about the same per cent, of divided tip ears as is produced by the divided tip parent. Divided tip is dominant in crosses to normal tip, but the domi- nance is not complete. In such cases there is the added difficulty of not being able to tell the recessive form from the dominant by inspection. Flattened cobs have proved dominant in crosses over cylindrical cobs. There are different degrees of cob division and in the extreme case the cob is grooved and only four rows are produced. Although we have not found this four-rowed condition to be inherited, there is no doubt of the fact that the tendency to division is sometimes inherited. We have often found the same pknt producing one ear which is four-rowed and another with eight rows. When the tendency to division is an inherited char- acter an inspection of the eight-rowed ear showed a tendency towards row splitting at the butt of the cob. In some cases we found this character to be non-inherited. Any row culture pro- ducing aver five per cent, of such ears should probably be discarded. Irregularity of Rows of Seeds The greater part of the ears of our commercial varieties of com have regular rows. Sometimes, as we have noted, two or more rows are dropped from a part of the ear, but even then the rows of seeds are regular and straight. Nearly all varieties produce some ears which show irregular rows, the kernels being packed closely together on the ear, making it almost impossible to count the row number. Such irregular ears are undesirable, the seeds presenting differences of size and shape which are not conducive to even dropping by cornplanters. Regular-rowed ears also present a more attractive appearance. Country Gentleman sweet corn is an irregular-rowed variety which proves that in some cases irregularity of rows is an inherited character. Irregular-rowed ears have often appeared 424 CONNECTICUT EXPERIMENT STATION REPORT, I91I. in our cultures and proved to be non-inherited, in all but one case. A self-fertilized, irregular-rowed ear of a white flint strain produced thirty-three normal and fifteen irregular ears, while a normal self-fertilized ear produced one hundred and twenty-five normal and five irregular ears. One of these five irregular ears was self-fertilized, and the following year produced fifty-six reg- ular ears and one irregular ear. A regular-rowed ear from the irregular-rowed strain produced seventy-nine regular and twenty- nine irregular-rowed. The manner of the inheritance of this character cannot be definitely stated, yet the character is an inherited one. Any commercial row culture which contains much over five per cent, of irregular ears should be discarded to eliminate the undesirable character. Summary of Results Corn is a normally cross-fertilized plant, and for this reason a commercial variety is composed of many types. Any variety can be made more uniform by selection. The benefit of selection seems chiefly due to the elimination of the poorer types, leaving only the better sorts. The improvement of corn by selection has been thoroughly dis- cussed in previous bulletins and will be only briefly mentioned here. Bulletin 152 gives directions for producing a breeding plot in which each row is grown from a single ear. Each row is then harvested separately, the yield and appearance determining the value of the selection. Such commercial row cultures have been and will continue to be of great value. We should not, however, expect too much from such a method. The increased yields which have been received by such methods are believed to be due to the elimination of undesirable types of which the pre- ceding abnormalities are examples. Row cultures are of ines- timable value in such work. In Bulletin 168 we discussed corn breeding methods and came to the conclusion that the growing of first generation hybrids would prove beneficial and materially increase corn yields. Some varieties will doubtless prove more beneficial for this work than others ; however, all investigators agree that crosses between highly selected sorts will prove most valuable. GENERAL SUMMARY. 425 It is hoped that the corn growers of Connecticut will not discard this article because of its technical nature and the diffi- culty of understanding this class of results. The subject of the inheritance of plant characters is a complex one, yet the seedsman or farmer who applies the known principles of inheritance and breeding to his own work will certainly receive a benefit thereby. PLATE XXV. a, Rhode Island white flint (starchy parent) ; b, Early Crosby (sweet parent) ; c, result of immediate cross of a and b, showing dominance of the flint type; d, result of planting seeds of c. The result of planting starchy seeds of d is shown by e, f, g. The result of planting sweet seeds is shown by /;. Pr.ATE XXVI. a, At left, upper ear, Jllmois low protein dent. Middle ear, immediate result of cross between low protein dent and Stowell's Evergreen sweet, and lower ear, Stowell's Evergreen sweet. At right, result of planting seeds of the hybrid ear. .u ' III n h i U n Hill fM I n It u II 1 1 II » b, At left, Rhode Island white flint; at right, Longfellow yellow (lint; in center, immediate result of cross between yellow and white flint, show- ing dominance of the yellow color. Lower ear is a self-fertilized ear grown from hybrid seeds of central ear. PLATE XXVII. ':3W a. At left, the color which develops in sunlight; in center, variegated color which does not breed true ; at right, common red pericarp. b, The second generation of a cross between the pericarp color which develops in the light and a white pericarp variety, giving, on the average, 3 colored ears to i white. The non-colored ear, if self-fertilized, will breed true. One out of every three, on the average, of self-fertilized colored ears will breed true. PLATE XXVIII. a, The first generation of a cross between a red and white pericarp, sliowing dominance of the red pericarp. The pericarp has lieen removed from two rows of seeds, sliowing a mixture of yellow and white endo- sperm colors. b, At left, Longfellow flint; at right, Illinois high-protein dent; in renter, result of growing a cross between them. PLATE XXIX. 1 a, The ear above is a self-fertilized dent ear, received from growing the hybrid shown in Plate IV, h (frequency about i in lo). Random sample of its progeny below. b, The ear above is a self-fertilized flint ear, received from i^rowing the hybrid shown in Plate IV', b (frequency about i in i6). Random sample of its progeny below. PLATE XXX, a, Tom Thumb pop, showing variation in length of ear. Length is given in even centimeters and the number of individuals in each class is given below (one-sixth of natural size). /.,-ff.i. /3 H iS lb /y /s /7 20 21 y.Ki. 3 11 u ;s lb' 7S 10 7 1 h. Black Mexican sweet, showing variation in length of ear (one-sixth of natural size). PLATE XXXI. i 7 b ISPI !3 "fSiii LervjtW lO II 1-: 14 /Vo, Va, / /I /£ /+ ,'7 ? a, Variation in length of ear of the first generation of a cross between Tom Thumi? and Black Mexican (one-sixth of natnral size). b. \ ariation in length of ear of second generation of cross between Tom Thnmb and lUack Mexican (one-sixth natural size). PLATE XXXII. A dwarf, appearing in a commercial culture of Stowell's Evergreen sweet, compared with a normal ear of the latter. INDEX. PAGE Abortion medicine .• 212 Acetanilid in headache powders 179 Acid phosphate, analyses of 39 Alabama (Aletia) argillacea 267, 339 Aletia argillacea 339 Alfalfa, notes regarding yield of 237 bread 212 meal 227, 250 American Agricultural Chemical Co. : — A. A. C. Co.'s Complete manure with 10% potash 62 Complete tobacco manure 62 (Carb.) 62 Grass and lawn top dressing 62 H. G. Fertilizer with 10% potash 62 Tobacco manure 62 Tobacco starter and grower 62 Bone meal 46 Dry ground fish 55 Fine ground bone 46, 48 Grass and oats fertilizer 54, 56 Ground tankage 6 and 30 50 Acid phosphate 40, 41 Castor pomace 27 Double manure salt 43 Genuine German kainit 2 H. G. dried blood 2 sulphate of potash 2 Muriate of potash 44 Nitrate of soda 23 Thomas phosphate powder 38 Bradley's Complete manure for potatoes and vegetables . . 62 top dressing grass and grain 62 with 10% potash 62 Corn phosphate 62 Eclipse phosphate 62 Farmers' new method fertilizer 62 Niagara phosphate 62 Potato fertilizer 62 manure 62 428 CONNECTICUT EXPERIMENT STATION REPORT, I9II. Page American Agricultural Chemical Co., cont'd: — Bradley's X. L. Superphosphate 62 Church's Fish and potash 62 Great Eastern General 62 H. G. Vegetable, vine and tobacco fertilizer 62 Northern corn special 62 North Western Fish, bone and potash 62 Market garden phosphate 62 Potato and truck guano 2 10% potato fertilizer 62 Universal fertilizer 62 Packers' Union Animal corn fertilizer 64 Gardeners' complete manure 64 Potato manure 64 Quinnipiac Climax phosphate 64 Corn manure 64 Market garden manure 64 Phosphate 64 Potato . manure 64 phosphate 64 Read's Practical potato special 64 Standard superphosphate 64 Vegetable and vine fertilizer 64 Wheeler's Corn fertilizer 64 Havana tobacco grower 64 Potato manure 64 Grass and oats 54-56 Williams & Clark's Americus Ammoniated bone super- phosphate 64 Corn phosphate 64 H. G. Special fertilizer . . 64 Potato manure 58, 64 Potato phosphate 64 Royal bone phosphate 2 Anthracnose of cherries and plums 398, 401 currants 405 Ants, how to get rid of 3^6 Aphiochata nigriceps 328 Aphis, apple, green 265 rosy 26s woolly 343 pine 343 Aphis pomi 265 sorbi 265 Apiary inspection 275 Apple aphis, green 265 rosy 265 INDEX. 429 Page Apple aphis, woolly 343 leaf crumpler 341 tent caterpillar 342 Apples, benefits of spraying 366 candied 202 details of spraying experiments 376 immune and susceptible varieties 359 leafburn 360 recommendations for spraying 370 results of two years' experiments 358 russeting 362 scald of 363 spray inj uries 360 Archips cerasivorana 309 Armour Fertilizer Works : — All soluble 58, 64 Ammoniated bone and potash 58, 64 Bidwell's Formula for all crops 58, 66 Bone, blood and potash 66 Complete potato 58, 66 Corn king 66 Fish and potash 58, 66 Fruit and root crop special 58, 66 High grade potato 58, 66 Market garden 66 Bone meal 46 5-35 Tankage 52, 53 German kainit 43 Nitrate of soda 23 Star phosphate 40, 41 Aspidiotus perniciosus 268 Atlantic Fertilizer Co. : — Atlantic Dissolved phosphate 40, 41 Atlantic gluten feed 222, 238 Atomic sulphur 356, 361, 369, 273, 375, 400, 402, 405 Baker Caster Oil Co. : — Pure castor pomace 37 Barberry hedges, a pest of 292 Basic slag, analyses of 38 Basilona imperialis 341 Beef scrap 231, 254 Beet pulp, dried 227, 250 molasses 227, 250 Berheris canadensis 292 Thunbergii 292 vulgaris 292 43° CONNECTICUT EXPERIMENT STATION REPORT, I9II. i' Page Berkshire Fertilizer Co. : — Berkshire Ammoniated bone phosphate 66 Complete fertilizer 66 Grass special 58, 66 Long Island special fertilizer 66 Potato and vegetable phosphate 66 Tobacco special (carb.) 66 Ground bone 46 Black knot of cherries and plums 399 Blatchford's calf meal 229, 252 Blissus leucopterus 265 Boardman, F. E. : — Boardman's Complete for potatoes and general crops 66 Bohl, Valentine : — Self recommending fertilizer 46 Bone, method of valuation 7 manures, analyses of 47 Bordeaux mixture 350, 361, 362, 369, 397 Bowker Fertilizer Co. : — Bowker's Bone and wood ash fertilizer 66 Complete alkaline tobacco grower 66 (carb.) 66, 82 Corn phosphate 66 Early potato manure 66 Farm and garden phosphate 66 Fisherman's brand fish and potash 66 Gloucester fish and potash 66 Highly nitrogenized mixture 2 Hill and drill phosphate 66 Lawn and garden dressing S8, 66 Market garden fertilizer 59, 66 Potato and vegetable fertilizer 66 phosphate 68 Special crop grower 2 Sure crop phosphate 68 Tobacco starter 68 Stockbridge Special complete manure for corn and all grain crops 68 Special complete manure for potatoes and vegetables 68 Special complete manure for seeding down, permanent dressing and legumes 59, 68 Special complete manure for tobacco 68 Special complete manure for top dressing and for forcing 59, 68 Bowker's Dry ground fish 55 INDEX. 431 Page Bowker Fertilizer Co., cont'd: — Bowker's Fresh ground bone 46 Market bone 46 Pure unleached Canada hardwood ashes 93) 94 Tobacco ash elements 59. 68, 82 Acid phosphate 40, 41 Muriate of potash 44 Nitrate of soda 23 Brandy 212 Brewers' grains, dried 227, 248 Brown rot of cherries and plums 402 Browntail moth, checking the spread of the 281 work, Killingly 285 Pomf ret 284 Putnam 283 Thompson 282 Woodstock 284 Buckwheat products 225, 248 Buffalo Fertilizer Co. : — Buffalo Celery and potato special 68 Farmers choice S8, 68 Fish guano 68 H. G. Manure 68, 82 New England special 68 Tobacco producer 68 Top dresser 60, 68 Vegetable and potato 68 Bone meal 46 Ground bone 46 Lime-kiln ashes 91 Tankage 49, 50 Butter and butter substitutes 195, 211 Buttermilk 210 Cocoa butter 163 Camphor liniment 162, 205 Castor pomace, analyses of 37 Chermes pinicorticis 343 pinifolicB 343 Cherries, experiments in spraying 398 recommendations for spraying 404 Cherry tent-maker 309 tortrix 309 Chili sauce 1 10 Chittenden, The E. D., Co. : — Chittenden's Complete tobacco and onion grower 68 Connecticut tobacco grower 68 Fish and potash 68 432 CONNECTICUT EXPERIMENT STATION REPORT, I9II. Page Chittenden, The E. D., Co., cont'd: — Chittenden's Potato and grain 70 manure 60, 70 Tobacco special 70 Chocolate 102 methods of analysis of 109 milk 108 sweet 104 Chop feeds 228, 250 Chrysanthemum leaf-miner 342 Chrysopa 289 Cicada, appearance of the periodical 2^6 Cider 112 Clark Seed Co., The Everett B. :— Clark's Special mixture for general use 58, 70 10% brand 58, 70 Clisiocampa americana 342 Cob meal 225, 246 Cocoa 114, 195 Cocoas, misbranded 124 Cod liver oil, methods of analysis of 170 wine of the extract of 171 emulsions 163, 205 Coe-Mortimer Co. : — E. Frank Coe's Celebrated special potato fertilizer 60, 70 Complete manure with 10% potash 70 Gold brand excelsior guano 58, 60, 70 H. G. Ammoniated bone superphosphate . . 70 New Englander corn and potato fertilizer 60, 70 Red brand excelsior guano 60, 70 Tobacco and onion special 70 Peruvian Tobacco fertilizer 70, 82 Vegetable grower 60, 70 XXX Pure ground bone 46 Muriate of potash 44 Nitrate of soda 23 Thomas phosphate powder 38 Coffee 211 cereal 160 Condensed milk 200 Connecticut Valley Orchard Co. : — C. V. O. Co.'s H. G. complete fertilizer 70, 84 Cooper's Fertilizer, Peter: — Peter Cooper's Pure bone dust 46 Copidosoma truncatella 328 Corn, see Maize. and cobmeal 225 INDEX. 433 Page Corn and oat feeds 228, 250 breeding, dominance of characters in 410 crosses between races differing in height of plants 419 having different row number 419 of flint and dent varieties 418 or dent with sweet varieties of 411 purple and white varieties of 413 yellow and white varieties of 412 having red cob, crosses with varieties having white cob . . . 415 pericarp, crosses with varieties having white pericarp 414 feed 225 inheritance in 407 of crosses not showing dominance 418 dwarf forms of 421 divided ears 422 irregularity of rows of 423 length of ears and size of seeds 420 summary of crosses showing dominance 416 Cotton hull ashes, analyses of 92 moth 267, 339 seed feed meal 221, 238 meal 220, 238 analyses of 25 Cracker waste ' 232 Cream 127, 195, 210 of tartar 160, 196 Currant worm 405 Currants, anthracnose of 405 the spraying of 405 Cutworm food 217 Cylindrosporium Podi 398 Cyllene rohinice 345 Dairy feeds 229, 252 Diabetic foods. See gluten preparations. Digestibility of feeds 233 Digestible nutrients purchasable for one dollar 234 Dioryctria abietella 341 Dissolved rock phosphate, analyses of 39 Distillers' grains, dried 227, 250 Dominance of characters 409 Dried blood, analyses of 24 Drug products, report on 162 Egg, dried 212 Eldredge, T. H. :— Eldredge's Special fish and potash fertilizer 70 superphosphate 70 434 CONNECTICUT EXPERIMENT STATION REPORT, IQII. Page Elm scale 344 tree food, analysis of 98 Entomosporium maculatum 396 Essex Fertilizer Co. : — Essex Complete manure for corn, grain and grass 70 potatoes, roots and vegetables 60, 72 Fertilizer for grass and top dressing 72 Market garden and potato manure 72 Special tobacco manure 72 Tobacco starter and grower 72 XXX Fish and potash 72 Ground bone 46 Euproctis chrysorrhcea 272, 281 Euvanessa antiopa 268 Exhibits of entomological department • 264 Expenditures of entomological department 259 Feeding stuffs, abstract of law concerning 219 Feeds, average composition and digestibility 235 digestibility of 233 summary of examinations of 232 Fertilizer elements, trade values of, in 191 1 6 Law, summary of I Fertilizer Materials Supply Co : — Acid phosphate 40, 41 Basic slag 39 Double manure salt 43 Dry ground fish 55 Muriate of potash 42, 44 Tankage 52, 53, 87 Tuttle's No. 4 corn mixture 72 Fertilizers, classification of 2 explanations concerning analyses 3 mixed, method of valuation of 8 number entered for sale i sampling and collection of 2 valuation of 4 Fish 211 analyses of dry ground and acidulated 54 Flies, how to get rid of 338 Flour 211 Food and drug products, summary of examinations of 215 products, report on loi Forest tent caterpillar 343 Frisbie, L. T., Co. : — Frisbie's Corn and grain fertilizer 72 Potato manure 72 Vegetable grower 60, 72 INDEX. 435 Page Frisbie, L. T., Co., cont'd: — Frisbie's Fine bone meal 46 Ground tankage 50 Acid phosphate 40, 41 Muriate of potash 44, 45 Nitrate of soda 23 Fusicladiufu pyrinum 396 Galerucella luteola 268 German Kali Works : — Kainit 43 Muriate of potash 44 Nitrate of soda 23 Sulphate of potash 43 Gloeosporhim Ribis 405 Gluten feed 224, 244 meal, analysis of 98 preparations 134, 196 Glycerophosphates, elixir of 212 Gossyparia spuria 344 Grape j nice 129 Gymnosporangium Japonicum 274 Gypsy moth, progress in controlling 277 work, cost of 280 Stonington infestation 278 Wallingford infestation 278 Hlair wastes, analyses of 98 Headache preparations 179, 205 Heredity, the law of 409 Hickory bark borer 341 Home mixtures, analyses of 88, 89 Hominy feed 224, 246 Horse feeds 228, 252 Husted's molasses feed 230, 254 steam cooked feed 229, 252 Ice cream 136, 195, 21 1 powders 137 Ichthyura inchisa 310 Imperial moth, parasite of 341 Inspection, apiary 275 nursery 269 imported stock 271 work, summary of entomological 264 Iodine, tincture of 180 James, Ernest L. : — James' ground bone 46 Jams 160, 211 Jtiniperiis chinensis var. compacta 274 43^ CONNECTICUT EXPERIMENT STATION REPORT, I9II, Page Juniperus tachikyaku 274 Kainit, analyses of 43 Ketchup 196 Lathromeris cicada 303 Latreilliniyia bifasciata 341 Lead arsenate, analyses of 218 effects of 352, 357 Lemon extract 196 Lemonade sugar 201 Leopard moth 266, 317 bibliography 334 summary 337 Leptinotarsa decemlineata 3H Lime, air-slaked, analyses of 89 and sulphur, self-boiled ...353, 361, 362, 373, 375, 400, 401, 402, 405 in various forms, analyses of 88, 89, 90 juice 129 water 181 Lime-kiln ashes, analyses of 91 Lime-sulphur, Blanchard's 352 Grasselli's 352, 362, 397 Niagara 353 Sherwin-Williams 353> 402 Sterling 353 Thomsen 353, 362 Vreeland 353 spray mixtures 35i Limestone, analyses of 88 Linseed meal 222, 238 Listers Agricultural Chemical Works : — Lister's Ammoniated dissolved bone phosphate 72 Complete tobacco manure 7^ (carb.) 72 Corn and potato fertilizer 72 Potato manure 7'^ Special grass mixture 72 10% fertilizer 7^ Standard pure bone superphosphate of lime 7^. Success fertilizer 72 3-6-10 for potatoes 72 Celebrated ground bone and tankage 50 Litomastix (Copidosoma) truncatella 328 Liquid manure, analyses of 9° Locust, appearance of seventeen-year 266, 296 borer 345 Mace ^96 INDEX. 437 Page Maize 224 meal 224, 246 red dog flour 225, 246 Malacosoma (Clisiocampa) americana 342 disstria 343 Malt sprouts 226, 248 Manchester, E., & Sons : — Manchester's Formula 72, 84 Special 72, 84 Mapes F. & P. G. Co. :— Average soil complete manure 58, 74 Cereal brand 58, 74 Complete manure "A" brand 58, 74 Corn manure 58, 74 Economical potato manure S8, 74 Fruit and vine manure 58, 74 Potato manure 58, 74 Seeding down manure 74 Tobacco ash constituents 74 manure, wrapper brand 74 starter, improved 58, 74 Top dresser improved, full strength 74 half strength 74 Vegetable or complete manure for light soils 58, 74 Dissolved bone 74 Maple leaf scale, woolly 345 leaf-stem borer 266, 305 sugar 211 Alarguerite fly 342 Marl, analyses of 92 Mash feeds for poultry 231, 254 Massaspora cicadina 304 Melalopha (Ichthyura) inclusa 310 Meltose 161 Mendel's law of heredity 408 Milk 138, 196, 210 adulterated 139 Mineola indiginella 342 Mocha-stone moth, poplar 310 Molasses 200, 211 feed, Quaker dairy 229, 230 Muck, analyses of 97 National Fertilizer Co. : — National Ammoniated bone phosphate 74 Complete corn and grain fertilizer 74 grass fertilizer 74 438 CONNECTICUT EXPERIMENT STATION REPORT, I9II. Page National Fertilizer Co., cont'd: — National Complete root fertilizer 60, 74 tobacco fertilizer 74 Connecticut Valley tobacco grower 74 Eureka potato fertilizer 74 Fish and potash 74 Formula "A" 76 Market garden fertilizer 76 Potato phosphate , 76 XXX Fish and potash 76 Tobacco special 76 (carb.) 76 Soluble bone and potash 54, 56 Dry ground fish 55 Ground bone 46, 48 Natural Guano Co. : — Sheep's head brand of pulverized sheep manure 95 New England Fertilizer Co. : — Corn and grain fertilizer 76 phosphate 76 H. G. Potato fertilizer 76 Perfect tobacco grower 76 Potato fertilizer 76 Superphosphate 76 Ground bone 46 Tankage 2 Niantic Menhaden Oil & Guano Co. : — Bone, fish and potash 76 Corn and grain fertilizer 76 H. G. Tobacco fertilizer 76 Market garden 76 Potato and vegetable manure 76 Sheepino natural guano 95 Acidulated fish guano 55 Dry ground fish guano 55 Nitrate Agencies Co. : — Peruvian guano 76 Acid phosphate 40, 41 Kainit 43 Muriate of potash 43 Nitrate of soda 23 Sulphate of potash 43 Nitrate of soda, analyses of 23 Nitrogen availability, pot experiments on 14 in mixed fertilizers, solubility of organic 56 raw materials, cost of 99 INDEX. 439 Page Nitrogen "inactive" 57 modified neutral permanganate method to determine solubility of 9 of raw materials, solubility of organic by the alkaline permanganate method 12 solubility of organic 8 Nitrogenous superphosphates, guaranties of 60 valuation of 61 and guanos, analyses of 62-85 Nitrous ether, spirit of 181, 205 Nurseries, inspection of Connecticut 269 Nursery firms receiving certificates in 191 1, list of 270 stock, inspection of imported , 271 Oat hulls 226, 248 Oats ^ 226, 248 Oil cake feed 222 Olds & Whipple :— O. & W.'s Complete tobacco fertilizer 76 Corn and potato 76 Fish and potash 76 Grass fertilizer 76 H. G. Potato fertilizer "^d Special phosphate 76 Dry ground fish 55 Grey pomace 37 Vegetable potash 46 Acid phosphate 40, 41 Carbonate of potash 45 Cotton hull ashes 92 H. G. Sulphate of potash 43 Lime 90 Precipitated bone 39 Tankage 50 Olive oil 200, 212 Omphalocera cariosa 294, 295 dcntosa 260, 263, 267, 292, 294, 295 Onion maggot 286 Opium 205 Orange preparations 129 sugar 134 Ortho-arsenite of zinc 346 Pamphilius persicum 314 Paprika 146 methods of analysis of 148 Parmenter & Polsey Fertilizer Co. : — P. & P. Grain grower 78 34 440 CONNECTICUT EXPERIMENT STATION REPORT, I9II. Page Parmenter & Polsey Fertilizer Co., cont'd: — P. & P. Plymouth Rock brand fertilizer 78 Potato fertilizer 78 grower 78 Special tobacco grower 78 Ground bone 46 Peach sawfly 314 Peaches, benefits of spraying 374 details of spraying experiments 391 recommendations for spraying 376 results of two years' experiments 371 spray injury to 372 Pears, experiments in spraying 396 recommendations for spraj-^ing 397 Pcgomyia ceparum 286 Pepsin 183 method of testing 185 Percentage difference, meaning and calculation of 84 Periodical cicada 296 Phenacoccus accricola 268, 345 Phorbia (Pegomyia) ceparum 286 Phosphates with potash salts, analyses of 54 Phosphoric acid in raw materials, cost of 100 Phytomysa chrysarJhemi 342 Pine aphids, v/oolly 343 Pissodes strobi 263, 268, 307 Plowrightia morbosa 399 Plums, experiments in spraying 398 recommendations for spraying 404 Phisia brassic(v 328 Poplar mocha-stone moth or tent-maker 310 Porthetria dispar 277 Potash, analyses of double sulphate of 43 high grade sulphate of 43 m.uriate of 43. 44 analj'^sis of carbonate of ^ 45 misbranded muriate of 45 in raw materials, cost of 100 Potassium iodide, determination of, in extract of sarsaparilla I93 Potato beetle, Colorado 311 Poultry feeds 230, 254 mash feeds 230, 254 Precipitated bone phosphate, analyses of 39 Priophorus accricaulis 266, 305 Protena dairy feed 230, 254 Pteronus ribesi 405 INDEX. 441 Page Pulverized Manure Co. : — Wizard brand manure 95 Purina feed 229, 252 Pyralid 292 Quinces, experiments in spraying 396 recommendations for spraying 398 Quinine pills 188, 210 Raspberry syrup 129, 196 Recessive characters 409 Red dog flour 223, 244 Relishes 151 Rhagoletis cingulata 399 Rosstelia atirantiaca 396 Rogers & Hubbard Co. : — Hubbard's "Bone Base" Complete phosphate 78 Grass and grain or fruit fertilizer 78 New mai-ket garden phosphate ... 78 Oats and top dressing 78 Potato phosphate 60, 78 Soluble corn and general crops manure 78 Soluble potato manure 78 tobacco manure 78 Pure raw knuckle bone flour 46 Strictly pure fine bone 46 Rogers Mfg. Co. : — All round fertilizer 78 Complete corn and onion 78 potato and vegetable 78 Fish and potash 78 H. G. Grass and grain 78 Oats and top dressing 78 Soluble tobacco 78 Tobacco and potato 78 grower 78 Tobacco starter 78 Pure fine ground bone 46 knuckle bone flour 46 Acid phosphate 40, 41 H. G. Sulphate of potash 43 Muriate of potash 43 Nitrate of soda 23 Root beer extract 150 Rum 212 Rust on juniper from Japan 274 Rye products 225, 248 Salad dressing 151 442 CONNECTICUT EXPERIMENT STATION REPORT, I9II. Page Sanderson Fertilizer & Chemical Co. : — Kelsey's Bone fish and potash 80, 84 Sanderson's Atlantic coast bone fish and potash 78 Corn superphosphate 80 Formula A 80 B 80 Potato manure 80 Special with 10% potash 80 Top dressing for grass and grain 80 Fine ground bone 46, 48 fish 55 Muriate of potash 43, 44 Nitrate of soda 23 Thomas slag 38 Special mixture 4-8-8 84 Sarsaparilla. extract of 189 Sauces, table 158 Schisoneura lanigera 343 Schreineria seuzera 328 Schumacher's calf meal 229, 230, 254 stock feed 230, 254 Scolytus quadrispinosus 341 Septoria pyricola 396 Seventeen-year locust in Connecticut in 191 1 296 Shay, The C. M., Fertilizer Co. : — Shay's Corn fertilizer 80 Grass fertilizer 80 Potato manure 80 Bone 46 Tankage 50 Acid phosphate 40, 41 Chrysanthemum fertilizer 98 Muriate of potash 44, 45 Nitrate of soda 23 Special mixture 80, 84 Sheep manure, analyses of 95 Shoddy waste, analyses of 98 Shoemaker, M. L., & Co. : — "Sv/ift-Sure" Superphosphate for general use 80 potatoes 80 truck, corn and onions .. 80 Bone meal 46 "Snuff" 210 Soda, analyses of nitrate of 23 water 200, 212 colors 202 INDEX. 443 Faee Soda water flavors 202 syrups 200 Soups, condensed 161 Sphecius speciosus 303 Spraj' mixtures, cost of 349 pumps 348 Sprayers, gasoline power 348 Sprays on apples, peaches, etc., tests of summer 347 Steero Bouillon Cubes 162 Stock feeds 229, 252 Sucrene dairy feed 230, 252 Sulphur and lead arsenate 357, 362, 367, 369 sprays, Bogart's sulphur compound 355, 362 One-for-all 355, 362 Sulfocide 355, 362, 369, 373 Swift's Lowell Fertilizer Co.: — Swift's Lowell Animal brand 80 Bone fertilizer 82 Empress brand 82 Potato manure 82 phosphate 82 Perfect tobacco grower 80 Potato grower 80 Special corn and vegetable manure 80 grass mixture 80 potato fertilizer 80 Superior fertilizer 80 Tobacco manure 80 Ground bone 46 Nitrate of soda 2 Tankage, analyses of slaughter house 49, 50 spurious 52 method of valuation 7 Tanner &: Wilcox : — Tanner & Wilcox' Reliable potato and garden phosphate . . 82 Tent caterpillar 342 Theobroma, oil of 163 Thomas slag, analyses of 38 Tibicen septendecini 296 Tmetocera ocellana 267 Tobacco ashes, analyses of 95 stems, analyses of 96 Totty's carnation fertilizer, analysis of 98 Trade values of fertilizer elements in 191 1 6 Unicorn dairy ration 229, 252 Union grains, Biles' ready ration 229, 252 444 CONNECTICUT EXPERIMENT STATION REPORT, I9II. Page Valuation of bone and tankage, method of 7 mixed fertilizers, method of 8 Valuations, explanations concerning 4 Vanilla extract 202 Vegetable potash, analysis of 46 Vinegar 155, 203, 212 methods of analysis of 157 Weather conditions, effect on spraying 363 Wells & Downs : — W. & D. Special economical 6-8-5 82 Wheat and corn feed 227, 252 cob feeds 228, 252 bran 222, 238 feed 223, 242 middlings 223, 240 Whiskey 212 White pine weevil 263, 268, 307 Wilcox Fertilizer Co. : — Wilcox Complete bone superphosphate 82 Fish and potash 82 4-8-10 fertilizer 82 Grass fertilizer 82 H. G. Fish and potash 82 Tobacco special 82 Potato fertilizer 82 onion and vegetable phosphate 82 Special superphosphate 82 Dry ground acidulated fish 54, 55 fish guano 55 Ground steamed bone 2 H. G. Tankage 49, 50 Pure ground bone 46 Acid phosphate 40, 41 Basic slag meal 38 H. G. Sulphate of potash 43 Muriate of potash 44 Nitrate of soda 23 Browning's formula 84 Fielden's formula i 84 2 84 1, grain and potatoes 84 2, grass 84 Wine of the extract of cod liver oil 171 Wirthmore rations 229, 252 Witch hazel, extract of 194 Wood ashes, analyses of 94 INDEX. 445 Page Woodruff, S. D., & Sons :— Woodruff's Home mixture 58, 82 Worcestershire and other sauces 158 Zeuzera asculi 317 pyrina 266, 317, 320 Zinc, ortho-arsenite of 346 analysis of 2i,7 « v,^ INDEX. 439 Page Nitrogen "inactive" 57 modified neutral permanganate method to determine solubility of 9 of raw materials, solubility of organic by the alkaline permanganate method 12 solubility of organic 8 Nitrogenous superphosphates, guaranties of 60 valuation of 61 and guanos, analyses of 62-85 Nitrous ether, spirit of 181, 205 Nurseries, inspection of Connecticut 269 Nursery firms receiving certificates in 191 1, list of 270 stock, inspection of imported 271 Oat hulls 226, 248 Oats 226, 248 Oil cake feed 222 Olds & Whipple :— O. & W.'s Complete tobacco fertilizer 76 Corn and potato 76 Fish and potash 76 Grass fertilizer 76 H. G. Potato fertilizer 76 Special phosphate 76 Dry ground fish 55 Grey pomace ^7 Vegetable potash 46 Acid phosphate 40, 41 Carbonate of potash 45 Cotton hull ashes 92 H. G. Sulphate of potash 43 Lime 90 Precipitated bone 39 Tankage 50 Olive oil 200, 212 Omphalocera cariosa 294, 295 deniosa 260, 263, 267, 292, 294, 295 Onion maggot 286 Opium 205 Orange preparations 129 sugar 134 Ortho-arsenite of zinc 346 Pamphilius persicum , 314 Paprika 146 methods of analysis of 148 Parmenter & Polsey Fertilizer Co. : — P. & P. Grain grower 78 34 440 CONNECTICUT EXPERIMENT STATION REPORT, I91I. Page Parmenter & Polsey Fertilizer Co., cont'd: — P. & P. Plymouth Rock brand fertilizer 78 Potato fertilizer 78 grower 78 Special tobacco grower 78 Ground bone 46 Peach sawfly 314 Peaches, benefits of spra3ang 374 details of spraying experiments 391 recommendations for spraying 376 results of two years' experiments 371 spray inj ury to 372 Pears, experiments in spraying 396 recommendations for spraj'ing 397 Pegomyia ceparum 286 Pepsin 183 method of testing 185 Percentage difference, meaning and calculation of 84 Periodical cicada 296 Phenacoccus acericola 268, 345 Phorhia {Pegomyia) ceparum 286 Phosphates with potash salts, analyses of 54 Phosphoric acid in raw materials, cost of 100 Phytomyza chrysanthemi 342 Pine aphids, woolly 343 Pissodes strobi 263, 268, 307 Plowrightia niorbosa 399 Plums, experiments in spraying 398 recommendations for spraying 404 Plusia brassiccc 328 Poplar mocha-stone moth or tent-maker 310 Porthetria dispar 277 Potash, analyses of double sulphate of 43 high grade sulphate of 43 muriate of 43t 44 analj'sis of carbonate of 45 misbranded muriate of 45 in raw materials, cost of 100 Potassium iodide, determination of, in extract of sarsaparilla .... I93 Potato beetle, Colorado 311 Poultry feeds 230, 254 mash feeds 230, 254 Precipitated bone phosphate, analyses of 39 Priophorus acericaulis 266, 305 Protena dairy feed 230, 254 Pteronus ribesi 405 INDEX. 441 Page Pulverized Manure Co. : — Wizard brand manure 95 Purina feed 229, 252 Pyralid 292 Quinces, experiments in spraying 396 recommendations for spraying 398 Quinine pills 188, 210 Raspberry syrup 129, 196 Recessive characters 409 Red dog flour 223, 244 Relishes 151 Rhagoletis cingulata 399 Rcestelia aurantiaca 396 Rogers & Hubbard Co. : — Hubbard's "Bone Base" Complete phosphate 78 Grass and grain or fruit fertilizer 78 New market garden phosphate ... 78 Oats and top dressing 78 Potato phosphate 60, 78 Soluble corn and general crops manure 78 Soluble potato manure 78 tobacco manure 78 Pure raw knuckle bone flour 46 Strictly pure fine bone 46 Rogers Mfg. Co.: — All round fertilizer 78 Complete corn and onion 78 potato and vegetable 78 Fish and potash 78 H. G. Grass and grain 78 Oats and top dressing 78 Soluble tobacco 78 Tobacco and potato 78 grower 78 Tobacco starter 78 Pure fine ground bone 46 knuckle bone flour 46 Acid phosphate 40, 41 H. G. Sulphate of potash 43 Muriate of potash 43 Nitrate of soda 23 Root beer extract 150 Rum 212 Rust on juniper from Japan 274 Rye products 225, 248 Salad dressing 151 442 CONNECTICUT EXPERIMENT STATION REPORT, I9II. ■ Page Sanderson Fertilizer & Chemical Co. : — Kelsey's Bone fish and potash 80, 84 Sanderson's Atlantic coast bone fish and potash 78 Corn superphosphate 80 Formula A 80 B 80 Potato manure 80 Special with 10% potash 80 Top dressing for grass and grain 80 Fine ground bone 46, 48 fish 55 Muriate of potash 43. 44 Nitrate of soda 23 Thomas slag 38 Special mixture 4-8-8 84 Sarsaparilla, extract of 189 Sauces, table 158 Schisoneura lanigera 343 Schreineria seusera 328 Schumacher's calf meal 229, 230, 254 stock feed 230, 254 Scolytus quadrispinosus 341 Septoria pyricola 396 Seventeen-year locust in Connecticut in 191 1 296 Shay, The C. M., Fertilizer Co. : — Shay's Corn fertilizer 80 Grass fertilizer 80 Potato manure 80 Bone 46 Tankage 50 Acid phosphate 40, 41 Chrysanthemum fertilizer 98 Muriate of potash 44. 45 Nitrate of soda 23 Special mixture 80, 84 Sheep manure, analyses of 95 Shoddy waste, analyses of 98 Shoemaker, M. L., & Co. : — "Swift-Sure" Superphosphate for general use 80 potatoes 80 truck, corn and onions .. 80 Bone meal 46 "Snuff" 210 Soda, analyses of nitrate of 23 water 200, 212 colors 202 INDEX. 443 Page Soda water flavors 202 syrups 200 Soups, condensed 161 Sphecius speciosus 303 Spray mixtures, cost of 349' pumps 348 Sprayers, gasoline power 348 Sprays on apples, peaches, etc., tests of summer 347 Steero Bouillon Cubes 162 Stock feeds 229, 252 Sucrene dairy feed 230, 252 Sulphur and lead arsenate 357, 362, 367, 369 sprays, Bogart's sulphur compound 355, 362 One-f or-all 355, 362 Sulfocide 355, 362, 369, 373 Swift's Lowell Fertilizer Co. : — Swift's Lowell Animal brand 80 Bone fertilizer 82 Empress brand 82 Potato manure 82 phosphate 82 Perfect tobacco grower 80 Potato grower 80 Special corn and vegetable manure 80 grass mixture '. 80 potato fertilizer 80 Superior fertilizer 80 Tobacco manure 80 Groimd bone 46 Nitrate of soda 2 Tankage, analyses of slaughter house 49, 50 spurious 52 method of valuation 7 Tanner & Wilcox: — Tanner & Wilcox' Reliable potato and garden phosphate , . 82 Tent caterpillar 342 Theobroma, oil of 163 Thomas slag, analyses of 38 Tibicen septendecim 296 Tmetocera ocellana 267 Tobacco ashes, analyses of 95 stems, analyses of 96 Totty's carnation fertilizer, analysis of 98 Trade values of fertilizer elements in 191 1 6 Unicorn dairy ration 229, 252 Union grains, Biles' ready ration 229, 252 444 CONNECTICUT EXPERIMENT STATION REPORT, I9II. Page Valuation of bone and tankage, method of 7 mixed fertilizers, method of 8 Valuations, explanations concerning 4 Vanilla extract 202 Vegetable potash, analysis of 46 Vinegar 155, 203, 212 methods of analysis of 157 Weather conditions, effect on spraying 363 Wells & Downs : — W. & D. Special economical 6-8-5 82 Wheat and corn feed 227, 252 cob feeds 228, 252 bran 222, 238 feed 223, 242 middlings 223, 240 Whiskey 212 White pine weevil 263, 268, 307 Wilcox Fertilizer Co. : — Wilcox Complete bone superphosphate 82 Fish and potash ' 82 4-8-10 fertilizer 82 Grass fertilizer 82 H. G. Fish and potash 82 Tobacco special 82 Potato fertilizer 82 onion and vegetable phosphate 82 Special superphosphate 82 Dry ground acidulated fish 54, 55 fish guano 55 Ground steamed bone 2 H. G. Tankage 49, 50 Pure ground bone 46 Acid phosphate 40, 41 Basic slag meal 38 H. G. Sulphate of potash 43 Muriate of potash 44 Nitrate of soda 23 Browning's formula 84 Fielden's formula i 84 2 84 1, grain and potatoes 84 2, grass 84 Wine of the extract of cod liver oil 171 Wirthmore rations 229, 252 Witch hazel, extract of 194 Wood ashes, analyses of 94 INDEX. 445 Page Woodruff, S. D., & Sons :— Woodruff's Home mixture 58, 82 Worcestershire and other sauces 158 Zeusera asculi 317 pyrina 266, 317, 320 Zinc, ortho-arsenite of 346 analysis of 217 State of Connecticut PUBLIC DOOUMEI^T JSTo. 24 Thirty-fifth Anntml Report OF The Connecticut Agricultural Experiment Station Being the annual report for the year ended October 31 1911 TRINTED BY ORDET{ OF THE LEGISLATURE HARTFORD Published by the State 1912 publication approved by The Board of Control. THE TUTTLE, MOREHOUSE & TAYLOR COMPANY CONNECTICUT AfiRICDLTURAL EIPERIMENT STATION, September 30, 191 1 BOARD OF CONTROL. His Excellency, Simeon E. Baldwin, ex officio. President. Prof. H. W. Conn, Vice President Middletown George A. Hopson, Secretary Wallingford E. H. Jenkins, Director and Treasurer New Haven Joseph W. Alsop • Avon Wilson H. Lee Orange Frank H. Stadtmueller Elmwood James H. Webb Hamden STATION STAFF. Administration. E. H. Jenkins, Ph.D., Director and Treasurer. Miss V. E. Cole, Librarian and Stenographer. Miss L. M. Brautlecht, Bookkeeper and Stenographer. Chemistry. Analytical Laboratory. John Phillips Street, M.S., Chemist in Charge. E. Monroe Bailey, Ph.D., C. B. Morrison, B.S., R. B. Roe, A.B., C. E. Shepard, Assistants. Hugo Lange, Laboratory Helper. V. L. Churchill, Sampling Agent. Proteid Research. Botany. Entomology. Forestry. Plant Breeding. Buildings and Grounds. T. B. Osborne, Ph.D., Chemist in Cliarge. Miss E. L. Ferry, A.B., Assistant. Miss Luva Francis, Stenographer. G. P. Clinton, S.D., Botanist. E. M. Stoddard, B.S., Assistant. Miss M. H. Jagger, Seed Analyst. Miss E. B. Whittlesey, Herbarium Assistant. W. E. Britton, Ph.D., Entomologist; also State Entomologist. B. H. Walden, B.Agr., D. J. Caffrey, B.S., A. B. Champlain, Assistants. Miss E. B. Whittlesey, Stenographer. Samuel N. Spring, M.F., Forester; also State Forester and State Forest Fire Warden. W. O. Filley, Assistant; also Asst. State Forester. Miss E. L. Avery, Stenographer. H. K. Hayes, B.S., Plant Breeder. C. D. HuBBELL, Assistant. ' William Veitch, In Charge. TABLE OF CONTENTS. PAGE Officers and Staff iii Contents v Report of Board of Control ix Report of Treasurer xv Errata xviii Report on Commercial Fertilizers I The Fertilizer Law and its Observance i Sampling and Collection of Fertilizers 2 Explanations concerning Analyses and Valuations 3 The Solubility of Organic Nitrogen 8 A Modified Method to determine Nitrogen Solubility g Solubility of the Organic Nitrogen of Raw Materials 12 Pot Experiments on Nitrogen Availability 14 Analyses of Raw Materials chiefly valuable for Nitrogen ... 23 Raw Materials chiefly valuable for Phosphoric Acid .... 38 Raw Materials chiefly valuable for Potash 42 Raw Materials containing Nitrogen and Phosphoric Acid 47 Mixtures of Phosphates with Potash Salts 54 Nitrogenous Superphosphates 56 Home Mixtures 86 Lime in various Forms 87 Cotton Hull and other Ashes 92 Miscellaneous Manures and Wastes 95 Report on Food Products loi Chocolate 102 Chili Sauce no Cider 112 Cocoa 114 Cream 127 Fruit Juices 129 Gluten Preparations 136 Ice Cream 136 Milk 138 Paprika 146 Root Beer, Relishes, Salad Dressing 150 Vinegar 155 Table Sauces and Miscellaneous 158 Report on Drugs 162 Camphor Liniment. Cocoa Butter 162 Cod Liver Oil and other Emulsions 163 Wine of the Extract of Cod Liver Oil 171 Headache Preparations 179 Iodine Tincture. Lime Water 180 vi CONNECTICUT EXPERIMENT STATION REPORT, I9II. PAGE Report on Drugs, cont'd. Spirit of Nitrous Ether 181 Pepsin 183 Quinine Pills and Compound Extract of Sarsaparilla 188 Extract of Witch Hazel 194 Food and Drug Products examined for the Dairy Commis- sioner 195 Miscellaneous Foods and Drugs 210 Summary of Results 215 Report on Commercial Feeding Stuffs 219 Law regarding Sale 219 Oil Seed Products 220 Wheat Products 222 Maize Products 224 Rye and Buckwheat Products 225 Oat and Barley Products 226 Miscellaneous Feeds 227 Proprietary Feeds 228 Digestibility of Feeds by Ruminants 233 Average Composition Digestibility and Selling Price of Feeds 233 Notes regarding the Yield of Alfalfa 237 Tables of Analyses of Feeds 238 Analyses of Bean Pods and Straw 258 Report of the Entomologist 259 Work of the Department 260 Entomological Features of 191 1 265 Inspection of Nurseries and Imported Stock 269 Inspection of Apiaries 275 Progress in Controlling the Gypsy Moth 277 Checking the Spread of the Brown-Tail Moth 281 The Onion Maggot 286 The Pyralid 292 The Seventeen- Year Locust 296 The Maple Leaf-Stem Borer 305 Preliminary Tests to Prevent Damage by the White Pine Weevil 307 The Cherry Tent Maker 309 The Poplar Mocha-Stone Moth 310 The Colorado Potato Beetle 311 The Peach Saw Fly 314 How to get rid of Ants 316 The Leopard Moth 317 How to get rid of Flies 338 Brief Insect Notes 339 Report on Tests of Summer Sprays on Apples, Quinces, etc 347 Types of Sprays used 349 General Results with Apples 3S8 TABLE OF CONTENTS. Vll Report on Tests of Summer Sprays on Apples, etc., cont'd. Recommendations 370 General Results with Peaches 371 Recommendations 375 Details of the Experiments 376 Experiments with Pears and Quinces 396 Cherries and Plums 398 Currants 405 Inheritance in Corn 407 Index 427 Report of the Board of Control OF THE CONNECTICUT AGRICULTURAL EXPERIMENT STATION. To His Excellency, Simeon E. Baldivin, Governor of Connecticut: The Board of Control of The Connecticut Agricultural Experi- ment Station herewith respectfully submits its report for the year ending November i, 191 1. The following minute regarding William Henry Brewer, for many years a member of this Board, was adopted by the Board at its meeting on December 22, 1910: Professor William H. Brewer, Ph.D., LL.D., a member of this Board for thirty-three years, died at his home in New Haven on November 2, 1910. His manifold work as geographer, botanist, sanitarian and teacher needs no recital here. As one of the pioneers in bringing agricultural science to the knowl- edge of farmers and teaching its value for practical ends, his name will be held in grateful remembrance by the farmers of this State. Born and brought up on a farm and having personal experience of farm work, he began his studies in Yale College with the object of fitting himself for the life of a farmer. Drawn by his tastes for natural science and eagerness to follow up the studies he had begun, he finished the regular course and graduated at the Sheffield Scientific School in 1852. After studying abroad at Paris, Heidelberg and Munich, serving as a college professor at Washington College, Pennsylvania, and the University of California, and working for four years on the survey of California, he returned to the Scientific School of Yale as professor of agriculture in 1864, and held that position until his retirement as professor emeritus in 1903. He was professor of agriculture not onlj^ within college halls but everywhere in the State. His intimate acquaintance with practical farm- ing, his scientific attainments, the wide range of his knowledge and of his interests, together with his broad common sense, made him a welcome and effective speaker at farmers' gatherings everywhere. He was earnest and influential in establishing this Agricultural Experi- ment Station and he served it wisely and effectively until he died. His last public act was to attend a meeting of this Board only a few days before his death. X CONNECTICUT EXPERIMENT STATION REPORT, I9II. Probably no one in the University during the last fifty years was called on so often and so variously for public service outside of the institution as Professor Brevi^er, and in all of it he was faithful, tactful and efficient. The Members of this Board wish here to record their sense of personal loss, their appreciation of his rare qualities as a friend and adviser, and of his long and effective labors for the advance of agriculture. It is Ordered that this minute be entered on the records of this Station and that a copy be sent to Professor Brewer's family. A detailed account of the work of each of the several depart- ments of the Station will appear in the reports of individual members of the staff, which are now in preparation. We therefore only call attention to certain facts relating to the Station welfare which will not be discussed in those publications. The General Assembly, at the January session of 1911, appro- priated $6,500 to this Station to cover the loss caused by the fire of January 10, 1910. Chapter 192 of the Public Acts of 191 1 provides for the printing of an annual report of this Station, which had been the practice for thirty years, in place of the biennial report required by statute during the last four years. Chapter 134 of the Public Acts of 191 1 requires that the net contents of all packages of food products shall be plainly marked on the outside in terms of weight, measure, or numerical count, and that the director of this Station, with the Dairy and Food Commissioner, shall establish rules and regulations as to the reasonable variations and allowances which shall be permitted. No penalty is to be enforced prior to eighteen months from the passage of this Act. Chapter 274 forbids adulteration of turpentine or spirits of turpentine and makes it the duty of the Dairy and Food Com- missioner and the director of this Station "acting jointly" to enforce this law. To the Commissioner alone is given the right of inspection. This statute, unlike any other relating to adultera- tion, makes the Station jointly responsible for enforcing the law. This is a departure from previous policy. Hitherto the Station's responsibility has been limited to testing suspected REPORT OF THE BOARD OF CONTROL. XI articles, reporting its findings to the Commissioner, and giving expert evidence in court when required. There have been no changes of importance in the Station staff except that Mr. Walter O. Filley has been appointed assistant state forester, an office created by the last General Assembly, and Mr. A. B. Champlain, an assistant in the entomological department, resigned October i, 191 1, to accept a position in the State Laboratory of Economic Zoology of Pennsylvania. During the fall, an exhibit illustrative of the several depart- ments of the Station work was made at six agricultural fairs and also at the Station grounds in New Haven, the last espe- cially for the teachers of the State who were attending a convention at the time in New Haven. Members of the Station staff were in constant attendance at all these exhibits to explain them and to answer questions. This involved the almost complete suspension of our regular laboratory work for nearly six weeks, which was a serious interruption. The large attend- ance, however, and the interest shown in the Station work as illus- trated by the exhibits convince us that this is one of the most effective ways of diffusing information regarding our w^ork among the farmers of the State. The Station has bought a farm of twenty acres at Mount Carmel, not far from the city, including a small house for the caretaker, has set out an orchard of apples and peaches for experiment, has begun an experiment on the effect both on the crop and on the soil of fertilizers and manures, and another, on the handling of an old and neglected orchard. At the Centerville field, which will be given up in the spring of 191 2, we have over 800,000 white pine seedlings, three years old, which are being sold at cost to citizens of this State for forest planting. On this field also com and tobacco breeding work, as well as other experiments, has been carried on during the present year. Hereafter this work will be concentrated on our own fields at Mount Carmel. The Station receives one-half of the so-called Adams Fund which, by Act of Congress and the rulings of the Secretary of Agriculture, must be used wholly for scientific research, prefera- bly in a small number of "projects" approved by the Office of XU CONNECTICUT EXPERIMENT STATION REPORT, I9II. Experiment Stations. The Station's share of this fund is devoted to two projects: a study of the laws of inheritance in maize and tobacco, and a study of the composition, structure and relative nutritive value of the vegetable proteins. This last pro- ject, which has engaged Dr. Osborne's time for many years, now receives very substantial aid from the Carnegie Institution of Washington. Bulletin 167, Inheritance in Maize, gives an account of five years' study of the subject, and Bulletin 168 is a discussion of the facts discovered which may be of immediate practical account. The results of work on the other project are published in physiological and chemical journals and in monographs of the Carnegie Institution and are not further noticed in this report. During the year there have been issued parts of a biennial report aggregating 512 pages with 21 plates in editions of 10,000 copies, and three bulletins aggregating 174 pages with 30 plates. Nine thousand five hundred copies of one of these were printed, of another three thousand, but of the third, which was so tech- nical as to be of value chiefly to investigators, only one thousand were printed. The spray calendar has also been revised to include the results of recent work and is mailed as requested. Fifty-one addresses have been given by members of the staff at farm institutes, field meetings, granges and other gatherings of farmers, and the Station correspondence has involved the writing of more than 9400 letters and manuscript reports. The following summary shows in brief the departments of the Station work and the special directions it has taken: Entomological Department. Inspection of all the nurseries in the State and of imported nursery stock to prevent distribution of insect and fungous pests ; inspection of apiaries on request or complaint ; gypsy moth con- trol work at Stonington and Wallingf ord ; . search for the brown- tail moth and destruction of its winter nests in northeastern Connecticut; studies of life-histories of certain insects, prepara- tion of exhibits; and publications in various journals on entomo- logical subjects ; also cooperation with the botanical department in the study of summer sprays, which is described below. REPORT OF THE BOARD OF CONTROL. Xlll Botanical Department. The preparation of artificial cultures of many fungi, most of them of economic importance, for purposes of investigation; studies on the oospore production of the potato blight in arti- ficial cultures and the relation of media to the stages and char- acter of growth of fungi in artificial cultures ; publication of , papers on botanical subjects; studies in the field of the chestnut blight, calico of tobacco and peach yellows ; tests of Millar's Cream muskmelon; and spraying experiments with melons, cucumbers and potatoes. In cooperation with the Entomological Department, very extensive and careful tests have been made to determine the comparative value of the various summer sprays on apples, peaches, pears, plums, cherries and some other fruits. The tests were made in thirteen orchards and involved the individual examination of 93,000 apples and about 25,000 peaches. Seed Testing. Tests of purity and vitality of field and garden seeds and identification of weed seeds for farmers and dealers ; and studies of methods of seed testing. Forestry Department. The care of the three Station nurseries, containing about 1,100,000 young trees for forest planting; care of the Station forest plantations, including forest planting, cleaning out worth- less species and liberation cutting; a test of basket willows; a demonstration planting of red and white pine at Putnam Memo- rial Camp Ground; inspection of State forests and destruction of pine weevil ; making fire lines and improvement thinning at the Portland forest ; the careful gathering of statistics of the 828 forest fires of the year which caused damage amounting to more than $235,000. New and desirable legislation has been secured regarding forest fires and the work of the forest fire wardens further systematized. Examinations of woodland for private owners have been made and advice given regarding planting, thinning and cutting. In cooperation with the U. S. Forest Service a study is being made of the woodworking industries of XIV CONNECTICUT EXPERIMENT STATION REPORT, I9II. the State, to get if possible a better utilization of home-grown woods and give the woodland owner information as to markets for his product. Chemical Department. Analyses and published reports of all commercial fertilizers sold in the State, of commercial feeds, and of human food pro- ducts and drugs ; examinations of foods and drugs for the Dairy and Food Commissioner, and expert evidence in court as required ; study of methods of analysis ; and analytical work required in connection with field experiments. Plant Breeding. (Supported by Adams Fund) Studies of inheritance of characters in corn and tobacco and of the yields of first year corn hybrids. Protein Research. (Supported by Adams Fund) Studies of the composition, structure and relative nutritive values of the vegetable proteins. The above list of the important parts of the Station work illustrates how it covers the whole State and affects the interests of all its citizens, v/hether farmers or not. Each session of the General Assembly adds to the State's requirements of the Station, the calls made by farmers and others for the help which it is the object of a Station to give, and the natural and inevitable expansion of its work have made the Station's income insufficient for its needs. The strictest economy will be necessary for the next year, and without a larger income than it now has the work which it is doing must thereafter be lessened and its working force cut down. For the general expenses of the Station no increase of appropriation has been made by the State since 1895. All of which is respecctfully submitted. George A. Hopson, Secretary. REPORT OF THE TREASURER, 1911 E. H. Jenkins, in account with The Connecticut Agricultural Experi- ment Station for the fiscal year ending September 30, 1911. Receipts. Balance on hand, October i, 1910 (State Agricultural Appropriation) $1,655.26 State Appropriation, Agriculture $10,000.00 State Appropriation, Food 2,500.00 State Appropriation, Insect Pest 3,000.00 State Appropriation, Gypsy Moth 4,000.00 United States Appropriation, Hatch 7,500.00 United States Appropriation, Adams 7,500.00 Analysis Fees 12,500.00 Sale of Farm Products 80.79 Miscellaneous Receipts 339.8i From the Lockwood Estate 16,611.23 64,031.83 Total $65,687.09 Disbursements. E. H. Jenkins, director, salary $2,800.00 E. H. Jenkins, treasurer, " 400.00 W. H. Brewer, salary 8.34 G. A. Hopson, " 75.00 V. E. Cole, " 850.00 L. M. Brautlecht, " 750.00 J. P. Street, " 2,500.00 T. B. Obsorne, " 2,400.00 E. M. Bailey, " 1,550.00 C. B. Morrison, " 1,158.34 R. B. Roe, " 1,093.75 C. E. Shepard, " 875.00 W. E. Britton, " 2,000.00 G. P. Clinton, " 2,200.00 E. M. Stoddard, " 758.32 S. N. Spring, " 2,500.00 W. O. Filley, " 1,030.73 H. K. Hayes, " 1,125.00 E. L. Ferry, " 1,033.32 H. Lange, " 925.00 V. L. Churchill, " 825.00 Wm. Veitch, " 675.00 Luva Francis, " 350.00 XVI CONNECTICUT EXPERIMENT STATION REPORT, I9II, E. L. Avery, salary $ 390.00 H. W. Kiley (Labor) 728.00 Wm. Pokrob " 728.00 C. D. Hubbell " 728.00 Geo. Graham " 724.00 M. H. Jagger "- 499.00 Jos. Keating " 180.00 L. Robinson " 475.00 L. Nolan " 173.00 Labor 3,842.79 Publications 1,137.82 Postage 357.71 Stationery 709.31 Telephone and Telegraph 161.69 Freight and Express 296.30 Gas, Kerosene and Electricity 545.28 Coal 1,583.00 Water 134-44 Chemicals and Laboratory Supplies 897.82 Agricultural and Horticultural Supplies 2,567.05 Miscellaneous Supplies 575-76 Fertilizers 480.51 Feeding Stufif s 188.98 Library and Periodicals 843.58 Tools and Machinery 334-31 Furniture and Fixtures 294.00 Scientific Apparatus 218.02 Live Stock 11.00 Traveling by the Board 118.11 Traveling by the Staff 1,295.91 Traveling in connection with Adams Fund Investi- gations 139.71 Fertilizer Sampling 231.60 Food Sampling 249.25 Insurance 65.80 Insect Pest Appropriation to State Entomologist .... 3,000.00 Contingent 572.51 Lockwood Expenses 400.00 Gypsy Moth Appropriation to State Entomologist . . . 4,000.00 New Buildings 396.90 Betterments 1,035.39 Repairs 337-14 Rental of Land 105.00 Purchase of Land 6,000.00 $65,634-49 Analysis Fees on hand Sept. 30, 191 1 52.60 Total $65,687.09 UAp'13 REPORT OF THE TREASURER. XVll New Haven, Conn., Nov. 14th, 191 1. This is to Certify that we have examined the accounts of E. H. Jenkins, Treasurer of The Connecticut Agricultural Experiment Station, for the year ending Sept. 30th, 191 1, have compared the same with the vouchers therefor and found them correct. William P. Bailey, Edward S. Roberts, Auditors of Public Accounts. E. H. Jenkins, in account with The Connecticut Agricultural Experi- ment Station for the fiscal year ending Sept. 30, 191 1, Receipts and Disbursements in connection with the rebuilding of the laboratory building destroyed by fire January 10, 1910. Receipts. Balance on hand Oct. i, 1910 $141.48 Salvage 5-89 $147-37 Disbursements. Fixtures $ 3.05 Apparatus, Laboratory Supplies and Miscellaneous 22.97 Part of interest on loan of $6,000 121.35 $147-37 This Certifies that we have examined the account which relates to loss by fire and rebuilding new building, have compared the same with the vouchers therefor and find them correct, the said account being closed and balanced by the expenditure of all moneys in the account, same being evidenced by vouchers on file and bank book. William P. Bailey, Edward S. Roberts, Auditors of Public Accounts. XVlil CONNECTICUT EXPERIMENT STATION REPORT, I9I I. ERRATA. Page 124, Stollwerck's Milk Cocoa is stated to contain glucose. This statement is an error. There is no evidence of the presence of glucose' or other adulterant in this brand. Page 200, nineteenth line from top, for henzoic read benzoic. Page 227, "The Biles' product" mentioned under Dried Dis- tillers' Grains refers only to rye grains, the analysis of which is given on page 250, and not at all to the other and higher grade brands of distillers' grains sold by the Biles Company. Page 229. The manufacturers state that Husted's Steam Cooked Feed contains only whole and cracked corn and whole oats but no wheat.