663 6 py 1 XTbe xaniverstt^ of Cbtcago SULPHUR AS A FACTOR IN SOIL FERTILITY A DISSERTATION SUBMITTED TO THE FACULTY or THE OGDEN GRADUATE SCHOOL OP SCIENCE IN CANDIDACY FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BOTANY BY JOHN WOODARD Private Edition, Distributed By THE UNIVERSITY OF CHICAGO LIBRARIES CHICAGO, ILLINOIS Reprinted from The Botanical Gazette, Vol. LXXIII, No. 2, February, 1922 Zbc Tllmversit^ of Cbicaoo SULPHUR AS A FACTOR IN SOIL FERTILITY A DISSERTATION SUBMITTED TO THE FACULTY OF THE OGDEN GRADUATE SCHOOL OF SCIENCE IN CANDIDACY FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BOTANY BY JOHN WOODARD Private Edition, Distributed By THE UNIVERSITY OF CHICAGO LIBRARIES CHICAGO, ILLINOIS Reprinted from The Botanical Gazette, Vol. LXXIII, No. 2, February, 1922 * — S -vi- . Gift Qciversity ^ .,-,-TTT NUMBER VOLUME LXXIII THE BOTANICAL GAZETTE February ig22 SULPHUR AS A FACTOR IN SOIL FERTILITY CONTRIBUTIONS FROM THE HULL BOTANICAL LABORATORY 289 John- Wood a r d Introduction Althou-h sulphur was recognized as an essential element in plant nutrition as early as the middle of the nineteenth century, the use of sulphur and sulphur compounds as fertilizers has never become general. Analyses for sulphur in soils have generally been low yet when compared with the sulphur in the ash of plants, the amount present in the soil seemed sufficient for all the needs of the crop. The use of gypsum as a fertihzer, however, was quite exten- sive for a time, following the discovery of its beneficial effect on plants. Browne (13) credits this discovery to a clergyman m Germany in 1768. From there it spread to France and Great Britain, and was brought to the United States by Benjamin Fr\nklin, who used it on his farm near Philadelphia. For a tmie gypsum was extensively used as a fertilizer both in Europe and the United States and gave remarkable results. Griffiths (25) reports experiments bv Schubert in Germany, and Crocker (15) refers to the experiments of Judge Peters, John Binns, and Edmund RUFFIN in the United States. All these men obtained remarkable results with gypsum on legumes. The use of gypsum alone, however, soon failed to mcrease crop yields, and investigators seeking for an explanation came to the conclusion that the gypsum acts chemically on the phosphorus or potassium compounds in the soil and liberates either phosphorus or 81 82 BOTAMCAL GAZI'.TTE [fkhriarv potassium or both. This view is presented by Griffiths (25), VooRHEES (72), and Hopkins (32). Browne (13) and Bruckner (14) consider the beneficial effect of gypsum due, in part at least, to the nutrient effect of the sulphur; while V'exdelmans (70) and PIilgard (31) mention its beneficial effects, particularly on the legumes, without giving any explanation. In most fertilizer experiments sulphur has been added, together with phosphorus, in acid phosphate or basic slag, or with the potassium in potassium sulphate or kainit. When beneficial results have been obtained, the investigators have invariably ignored the possible effects of the sulphur. This may lead to erroneous con- clusions, as was pointed out by Liebig (37) in 1855. He said that the sulphur or the calcium in the acid phosphate, or both, might have had a beneficial effect on the turnips in the Rothamsted experiments, as well as the phosphorus. Hopkins, Mosier, Pettit, and Reauhimer (33) found that kainit increased the yields of corn, wheat, and oats on the waste hill land of Johnson County, Illinois, when used with bonemeal, ground limestone, and crop residues, over similarly treated plots without kainit. On the plots receiving no kainit, as well as on those receiving the kainit, cowpeas were grown once every three years and turned under as part of the crop residues. Stewart (66) compared potassium chloride and potassium sulphate as fertilizers for apple orchards in Pennsylvania. He found no appreciable difference in the effect of these salts. Smith (65) found a greater yield of oat straw for potassium sulphate than potassium chloride in pots containing Hagerstown silt loam. Brooks (8) compared the effects of potassium sulphate and potassium chloride on alfalfa in field experiments at the Massachu- setts Agricultural Experiment Station. Both ])lots received 600 pounds of bonemeal per annum, and both received 2 tons per acre of hydrated hme before planting the alfalfa. Both Grimm alfalfa and common alfalfa w^ere used. Potassium sulphate gave increased yields of 0.50 tons of Grimm alfalfa and 0.75 tons of com- mon alfalfa over potassium chloride. In every case the alfalfa on the plots receiving potassium sulphate was a darker green than on the plots receiving potassium chloride. The same difference in color 1922] WOODARD—SOIL FERTILITY 83 was reported for the same treatment on other crops. Brooks (9) also made a comparison of different phosphate fertihzers. He found that acid phosphate and dissolved boneblack, which contain sulphur, gave greater increases in crop yields than raw bonemeal and rock phosphate, which contain little or no sulphur. A more rapid early growth of both tops and roots and earlier maturity were observed on the plots receiving the dissolved boneblack and acid phosphate than on the plots receiving raw bonemeal and rock phosphate. The use of flowers of sulphur as a fert'lizer was observed to have an influence aside from its effect in destroying the fungi which cause plant diseases. Mares (50) noticed a much greater vigor in vines that had been sulphured than in those which had not. He found that the sulphur was oxidized to sulphuric acid in the soil, and he thought that the sulphuric acid acted on the insoluble compounds containing potassium and made the potassium soluble. Demolon (16) found that heating the soil prevented the oxida- tion, and so he concluded that oxidation was caused by micro- organisms. Pfeiffer and Blanck (56) obtained no increased yields of oats for the use of flowers of sulphur in field experiments. Feilitzen (21) in Europe, and Sherbakoff (64) in the United States both obtained increased yields of potatoes from the use of flowers of sulphur. Boullanger and Dugardin (3) found flowers of sulphur in- creased ammonification but decreased nitrification. The harmful effect on the nitrifying bacteria was probably due to the acidity, as Lint (38) found that the oxidation of sulphur in the soil in- creased the acidity very much. Fred and Hart (23) report an increase in ammonification from the use of gypsum in peptone so- lutions, and Warington (73) obtained an increase in nitrification when gypsum was applied to solutions of urea. Greaves, Carter, and GoLDTHORPE (24) studied the influence of calcium sulphate on production of nitrates and found it caused a great increase in all concentrations used. The increase was very high for the higher concentrations of calcium sulphate. Brioux and Guerbet (7) found that flowers of sulphur increased availability of calcium and potassium in both calcareous §4 BOTAMCM. GAZETTE [febrl-.\ry and noncalcareoLis soils, but had no effect on phosphorus. LiP- MAN' and McLean (42) found that composting rock phosphate with sulphur increased the solubilit\- of phosphorus. AIcLkax (48) found an increase of solubility of phosphorus in the sulphur- rock phosphate compost when compost was inoculated. The presence of soluble phosphates and sulphates did not inhibit the action. Lipmax, McLean, and Lint (43) found a great increase in acidity in the sulphur-floats mixture. Lipaian and Joffe (41) found no increased availability in phosphorus when acidity was increased by the addition of sulphuric acid. Ellett and Harris (20) found greater availability of phosphorus in a manure-soil- floats-sulphur compost than in a soil-floats-sulphur compost. Ames and Richmond (2) found no increased availability of phosphorus in a compost to which calcium carbonate had been added. Acid conditions are necessary for the solution of the phosphorus. Brown and Gwinn (id) found an increased solu- bility of phosphorus in soil treated with sulphur as well as in com- posts. Brown and Warner (12) found no increased solubility of phosphorus in a manure-floats compost, but a great increase w'hen flowers of sulphur were added to the compost. The use of gypsum as a preservative of the nitrogen in manure has been investigated by Heinricii (30), Vivien (71). Xolte (53), and by Ames and Richmond (i). All these investigators report a saving of nitrogen from the use of gypsum on the manure. Investigations on the effect of flowers of sulphur on the a\ail- ability of potassium in greensands were conducted by McCall and Smith '45^. They found an increase in the a\ailability of potassium in composts of sulphur, greensands, and manure, but no increase in a\-ailability of potassium in composts of sulphur, greensands, and soil. Reports of investigators who studied the influence of gypsum on the availability of potassium do not agree. ]\rcCooL and Millar (46.) found calcium sul[)hate api)lied to soil lowered the freezing point of the soil. Xo rej)ort was gi\-en as to the character of the compounds that lowered the freezing point. Bradley (4) found an increase in solubility of potassium but not of phosphorus in Oregon soils. Hriocs and Brezkale (6) found a decrease in 1922] WOODARD—SOIL FERTILITY 85 solubility of potassium in California soils when gypsum was added, and the solubility of potassium decreased as the amount of gypsum used was increased. Brezeale and Briggs (5) grew wheat in water cultures, using extracts from orthoclase minerals with and without gypsum. The gypsum did not increase the availabihty of the potassium to the wheat. Morse and Curry (52) treated feldspars with gypsum for ten weeks in water, filtered oft" the solu- tion and analyzed for potassium. Only sHghtly more potassium was found than when no gypsum was used. McMillar (49) treated five different soils with gypsum for three months and analyzed for soluble potassium. Gypsum was used at the rate of ten tons per acre and resulted in an increase in soluble potassium in every case. Tressler (69) found an increase in soluble potassium in some soils, but no increase in others when treated with gypsum. Lipman and Gericke (39) obtained an increase of available potassium in greenhouse soil, a shght increase in adobe soil, and no increase in sand. Fraps (22) grew plants in pots of soil treated with gypsum and analyzed the plants for potassium. He found no increase in potassium in plants grown on the gypsum-treated soil above that on the soil without gypsum. He reports no analyses of the soils used, however, so it is not known whether these soils were deficient in potassium or not. If the soil has sufficient potassium in an available form to supply all the plants' needs, there would not likely be any increased absorption even if the soil treatment dis- solved some of the insoluble potassium compounds in the soil. On the other hand, in a soil deficient in potassium and sulphur, the application of gypsum or any other fertihzer containing sulphur would stimulate the growth of roots, and the increased size of the root system would make it possible for the plant to absorb more potassium. This increased absorption would take place regardless of any possible eftects on the solubility of the potassium compounds in the soil. The experiments of McMillar (49), Tressler (69), and Lipman (39) indicate a greater solubihty of potassium in some soils when treated with gypsum, but other soils show no effect, while Briggs and Brezeale (6) report a decrease in solubihty when gypsum was used. It seems, therefore, that the beneficial 86 IU)TA\ICAL GAZETTE [fkbrl-ary elTects of gypsum can hardlv- he ascribed to its effect on the solu- bilit>' of the potassium in the soil. It seems more likely that the soils that respond to the use of <:;>{)sum are delicient in some element that is supplied by the gypsum. Recent studies of methods for the analysis of organic material for sulphur have shown that all the sulphur is not recovered in the ash when organic material is burned. H.\rt and Peterson (27, 28) found one hunflred times as much SO, in the rice grain as in the ash of that grain, and forty times as much in the corn grain. Similar results were obtained with other grains, but the ratios were less in some cases. Onions, potatoes, crucifers, and legumes use large quantities of sulphur. Alfalfa removes twice as much sulphur as phosphorus from the soil. Peterson (55) studied the sulphur compounds in plants and found proteins, volatile compounds, mustard oils, and sulphates. In ashing the plant material the sulphates remain, but at best part of the sulphur in other com- pounds is lost. IMost soils are low in sulphur, which is present in the soil in the form of sulphates and organic matter. Sulphates are all soluble, and, like nitrates, they are not adsorbed to any great extent, and therefore are quickly leached out of the soil in the humid regions. The organic sulphur is insoluble but is readily oxidized to sulphates, so that it is gradually being lost unless taken up by the plant. Lvox and Bizzell (44) in their lysimeter studies at Cornell found that the loss of sulphur in the drainage from uncropped lysimeters was as great as the loss in drainage and in the crops from cropped soil. The oxidation of organic sulphur to sulphates seemed to continue at the same rate in cropped and uncropped soil, and that not taken up by plants was lost in the drainage. Cultivation stimulates oxidation and consequently the loss of sulphur. S W.ANSON and Miller (68) report a loss of 38.53 per cent of sulphur from the surface and 41.56 per cent from the sub- soil of Kansas soils due to cropping. The surface soil of virgin land had 0.044 per cent sulphur, while adjoining cropped land had 0.027 per cent. The sulj)hur content of the subsoil was 0.062 per cent in the virgin land and 0.036 per cent in the cropped land. On the other hand, phosphorus was j)ractically the same in the 192 2] WOOD A RD— SOIL FERTILITY 87 cropped as in the virgin land in both surface and subsoil. The cultivated soils had been cropped for thirty to forty years. Lyon and Bizzell (44) found an increased loss of sulphur in the drainage when burnt lime was used, while MacIntire, Willis, and Holding (47) found the loss greater for calcium carbonate than for calcium oxide. It seems the carbonate favors bacterial action much more than the oxide. Robinson (59, 60) analyzed a large number of soil samples from different parts of the United States for sulphur and phos- phorus. Most of them were low, some extremely low, in both phosphorus and sulphur. Many of the samples were much lower in sulphur than phosphorus. Brown and Kellogg (ii) analyzed samples of Iowa soils and found the sulphur content varied from 719 to 938 pounds per acre in the surface soil, while the phosphorus content varied from 1289 to 1538 pounds per acre. Shedd (62) analyzed samples of Kentucky soils and found the sulphur content in the surface soil varied from 213 to 1080 pounds per acre in virgin soil, and from 180 to 560 pounds per acre in cultivated soils. The phosphorus content in the surface soil ranged from 320 to 5860 pounds in virgin soil, and from 320 to 7240 pounds in cultivated soil. Some sulphur is brought down from the air in rain water. The amount is probably greater during periods of heavy rainfall than when the precipitation is slight. Near cities, where a large amount of coal is burned, the amount is probably much greater than in country districts far from cities and railroads. The data, however, are too meager to form any definite conclusions. Hall (26) reports sulphur analyses of rain water at Rothamsted from 1881 to 1887 which give an annual average of seven pounds of sulphur in the rainwater per acre per year. Analyses by Hart and Peterson (27) at the University of Wisconsin for part of a year led them to the conclusion that the amount in one year would be approximately the same as found at Rothamsted. Stewart (67) analyzed rain water at the University of Illinois and obtained as a seven-year average 45.1 pounds of sulphur per annum. All of these analyses are of rain water collected near cities. The water in the rain gauges is likely to be contaminated by dust and soot and by the droppings of birds which roost on the rain gauges. 88 BOTA XICA L GA ZE TTE FKBRUARY Laan'KS and Ciii.iiKRT (36) found, in tlu'ir fertilizer c\j)criments with red clo\-er, that "the ]>rc)duce was considerably increased by the apphcation of gypsum, and still more so by that of the sul- phates of potash, soda, and magnesia, and superphosphate of lime." In four years the increased }-ield from the use of gypsum was 3.5 tons of dry ha>'. or an a\-erage of 0.9 ton per acre ])er year. IlrxT (35). at the Pennsylvania Agricultural I-lxjieriment Sta- tion, used gyj)sum in a rotation of corn, oats, wheat, and hay (timothy and clover). (lypsum was applied at the rate of 320 pounds per acre per rotation in two applications, 160 i)0unds to the corn and 160 pounds to the wheat. Xo other fertilizers were used, and no increases in }'ields were obtained from the use of g}psum. 'Jdiese experiments would be more valuable if the gypsum had been ai)plied to the clover anfl other fertilizers had been used to remove the possibility of another limiting factor. Miller (51) grew clover in pots containing Oregon soils. Applications of sulphur were made in the form of flowers of sul- phur, sodium sulphate, and gypsum. (i}j)sum and sodium sul])hate gave increased \'ields, but the llowers of suli)hur had little effect. SciiREiXER (61) studied the effect of different salts on oxida- tion in soil extracts in which wdieat seedlings were grown. He reports increased oxidation from the use of calcium suljihate, potassium sulphate, and sodium sul]:)hate. DvMoxD, Hughes, and Jupe (18) comi)ared the effect of ammonium sulphate and ammonium chloride on cabbages grown on non-calcareous soil. Oreater yields were obtained with the ammonium suli)hate tlian with the ammonium chloride. In their experiments with clover they obtained a 20 per cent increase in hay from the use of g>psum. In pastures they observed that legumes predominated where sulphates were aj^plied, and grasses where no sulphates were used, (jyj)sum increased the A'ields of red clover, maize, and vetch in sand cultures, and of vetch in soil cultures. All the pots received applications of calcium and mag- nesium carbonates. LiPM.AX and Gericke (40) compared the effects of different nitrogenous fertilizers on barley grown on Oakley's vitro sand, and found the greatest increase with ammonium sulphate. When 1922] WOOD ARD— SOIL FERTILITY 89 sulphur containing substances were added to the non-sulphur containing nitrogenous fertilizers, they produced yields equal to those from ammonium sulphate. Shedd (63) grew soy beans, oats, alfalfa, and wheat in pots containing Kentucky soils. Eight different soils were used, and flowers of sulphur added at the rate of 100 and 200 pounds per acre. Both controls and sulphur treated pots received tricalcium phosphate, potassium nitrate, and calcium carbonate. There were some increases but also some decreases. Eaton (19) grew sweet corn in pots containing sand. He compared the effect of gypsum, flowers of sulphur, and sodium sulphate. The controls as well as the different sulphur treatments were watered with a nutrient solution which contained no sulphur. Gypsum increased the yield, while flowers of sulphur and sodium sulphate gaye increases for the smaller applications and decreases for the larger applications. DULEY (17) reported a darker green in sweet clover and corn when fertilized with gypsum or sulphur. More nodules were also produced on the roots. PiTZ (57) grew clover in agar-agar containing dipotassmm phosphate with and without calcium sulphate. Greater length of roots was obtained with the calcium sulphate. Clover was also grown in Miami silt loam with and without calcium sulphate. The calcium sulphate increased the root length. Hart and Tottingh.^m (29) found a decided increase in develop- ment of beans, red clover, and peas when fertiUzed with either calcium sulphate or sodium sulphate. In beans and peas the increase was in the seed, in clover it was in the hay and roots. Sulphates increased the yields of both tops and roots in radishes. The yield of rape tops was increased by both calcium and sodium sulphates. Barley was not affected by the sulphates, and oats to only a slight extent. Olson (54) conducted field e.xperiments with alfalfa at the Washington Agricultural Experiment Station and obtained in- creased yields from the use of acid phosphate and gypsum, but not from other forms of phosphorus. Two hundred pounds ot g^-psum per acre increased the yields of alfalfa from 100 to 500 per cent. 9© BOTAXICAI. CAZETTE [ferriary Rkimfr and Tartar (58) conducted held experiments on sev- eral Oregon soils. Superphosphate, flowers of sulphur, rock phos- phate, potassium chloride, potassium sulj)hate, iron sulphate, <^'psum, monocalcium jihosphate, sodium nitrate, ammonium suli)hate, magnesium sul])hale, sodium sulphate, iron pyrites, Cjuick lime, and ground limestone were used as fertilizers. In almost every case enormous increases in fields (from two to ten times as much as the checks) were obtained for all the fertilizers containing sulphur, and no increase or only a small increase for the fertihzers which contained no sulphur. Acid ])hosphate was compared with gy])sum and rock j)hosj)hate and with rock phosphate and tlowers of suli)hur. The \ield on the plot recei\ing rock phosphate and gypsum was consi(leral)ly greater, and that from the plot receiving rock phosphate and flowers of sulphur slightly greater, than the yield from the acid phosphate treated plot. The alfalfa on all the i)lots receiving sulphur in any form was a darker green than on the plots which received no sulphur. Chemical analyses of soil samples from these exi)erimental lields were made. The sulphur content varied from 0.015 to 0.038 per cent in the surface soil, and from 0.014 to 0.030 i)er cent in the subsoil. The phosphorus content varied from 0.048 to 0.076 per cent in the surface, and from 0.066 to 0.085 pcr cent in the subsoil. AH were high in calcium, magnesium, and j)otassiun-i. Investigation The analyses made by Rouixsox (59, 60) show wide variation in the sulphur content oi dilTerent soil types. His investigations, although extensive, have included onl\- a part of the numerous soil types found in the United States, so that other soil tyj^es should l^e anal}'zed to discover their suli)hur as well as their j)ho.'^j)horus content. It is also necessary to conduct held experiments on the different soils, as analytical data alone arc not sufficient evidence on which to base fertilizer practice. This investigation includes soil analyses and fiekl experiments. Soil samples from Indiana, Kentucky, ^Michigan, Ohio, and Wisconsin were analyzed for phosphorus, sulphur, and volatile matter (loss on ignition). Field 1922] WOODARDSOIL FERTILITY QI experiments were conducted in Indiana and Kentucky on the fields from which the soil samples were taken. Soil analysis Methods of sampling.— The soil samples from Michigan and Ohio (nos. 1-9) were taken by Dr. William Crocker and those from Wisconsin (nos. lo-ii) by Mr. E. H. Hall. The samples were taken in the usual way by means of a soil auger. The samples from Indiana and Kentucky were taken when the soil was very wet, and as only the surface soil was sampled, it was beheved that more accurate samphng could be done by using a spade or shovel. Some soil was removed to a depth of seven inches, leaving one side of the hole vertical, then a thin shce of soil was cut with the spade to the full depth of seven inches. A narrow strip of this extending from top to bottom was removed for the sample. Three or four such samples from different parts of the field were taken and mixed to form a composite sample. The samples from Indiana were taken by John Woodard. except no. 18, which was taken by Mr. V. G. Mann, and those from Kentucky by John Woodard, except nos. 32-34, which were taken by Mr. J. C. Gentry. All the soil samples were air dried, sifted through a 2 mm. sieve, and thoroughly mixed. Analytical methods. Phosphorus was determined according to the oflicial magnesium nitrate method of the Official Agricultural Chemists. A blank determination was run to determine the possible presence of phosphorus in the chemicals, but no phos- phorus was found. Sulphur was determined by a modification of the methods of Shedd and of Brown and Kellogg. In preliminary work it was found that higher results were obtained when the iron and aluminum were removed. In soils low in sulphur the barium sulphate pre- cipitated very slowly, so, at the suggestion of Dr. Frederick Koch,' 10 cc. of approximately N/io H,SO, was added immedi- ately before heating the solution and adding the barium chloride. This sulphuric acid was measured in a burette, and exactly the ' Unpublished work of Dr. Frederick Koch. 92 BOTAXICAL CA/r.TTE [fkijrcary same ([uaiilit}' of the same acid was added lo the l)Iank determina- tion, so that subtracting the bhink subtracted the sulphur added in the sulphuric acid as well as that present in the reagents. In every case the lo cc. was measured between the lo and 20 marks on the burette. According to Kocii, barium sulphate does not precipitate readily when the concentration of the SO^ ion is low. The addition of the sulphuric acid is then necessary to bring the concentration of the SO4 ion up to the j^oint where precipitation takes place readily. The method as linally adopted is as follows: The ecjuivalent of 10 gm. of oven dry soil was weighed into a nickel crucible, moistened with a few drops of distilled water, and ])art of a weighed 20 gm. of sodium {"jcro-xide stirred in a little at a time with a nickel rod. (If the moisture was just right, reaction took place immediately without the application of heat, and the charge was fairly dry by the time most of the sodium pero.xide had been stirred in. If too little water had been added, it was neces- sary to heat with an alcohol lamj) to start the reaction. If too much water was added, it was necessary to heat with the alcohol lamp to bring lo the desired degree of dryness before adding the last of the sodium pero.xide.) After the charge was fairly dr}-. the rest of the sodium peroxide was placed o\-er the charge, the crucible covered, and heated o\er a bunsen burner, raising the temperature gradually to a fairly high temperature which was maintained for an hour. After cooling, the fused mass was remo\ed with hot dis- tilled water to a 600 cc. beaker, neutralized with concentrated HCl, and then 10 cc. additional concentrated HCl added. The beaker was then heated for tive or six hours on the steam bath with occasional stirring. It was then transferretl to a 500 cc. tlask, covered, and made uf) to the mark. The solution was shaken frequently for several hours and the 250 cc. tiltered olY. The 250 cc. of tiltrate was transferred to a 600 cc. beaker, heated on the steam bath, and the iron, aluminium, and silica precipitated with ammonium hydroxide, allowed to stand a few minutes, and then llltered into a one liter beaker. The precipitate was washed with hot distilled water until the combined filtrate and washings had a \-olume of approximately 600 cc. Exactly 10 cc. of approx- imatelv X'lo H.SO, was then added, heated to boilimr. and 10 cc. 19-2-^] WOODARD—SOIL FERTILITY 93 of hot lo per cent BaCl, solution added a drop at a time from a pipette. The sohition was boiled for ten minutes, placed on the steam bath for two or three hours, and then removed and allowed to stand over night. The barium sulphate precipitate was then filtered off, washed with cold distilled water, transferred to a weighed porcelain crucible, ignited to a dull red in a muffle furnace, cooled in a desiccator, and weighed. Blanks were determined using the same reagents and adding the same quality of the same sulphuric acid that was used in the determination. The loss on ignitioii was determined on samples which had been used for determining moisture. The moisture was deter- mined by heating lo gm. of air dry soil in the oven for five or six hours. Part of the samples were heated to ioo° C. in an ordinary oven and part of them to 35° C. in a vacuum oven. After weighing for the moisture determination, the sample was placed in the mufl^e furnace, heated to a dull red for an hour, cooled in a desic- cator, and weighed. The loss on ignition was calculated as percent- age of oven dry soil. Table I gives the results of the analytical work on all the soils analyzed. Phosphorus, sulphur, and volatile matter (loss on ignition) are reported as percentage of oven dry soil. Sulphur is present in the soil either in the form of sulphates of calcium, magnesium, and iron, or in the form of organic matter. All the sulphates are quite soluble and are not readily adsorbed, so that they are leached out rapidly and only small amounts are present in the soil. On the other hand, the organic sulphur is insoluble and remains in the soil until oxidized to sulphates. One would expect, therefore, some sort of relation between the sulphur content of the soil and the volatile matter (loss on ignition), which is a rough method of determining the organic matter. The data in table I, however, indicate only a general relation, and that only when samples from the same soil type or closely related soil types are compared. The soil samples from Wisconsin are from the same soil type, but differ in amount of organic matter. There is also a difference in content of sulphur, and the higher sulphur con- tent is found in the sample with the higher content of organic mat- ter. This is true for both surface soil and subsoil. The Michigan 94 BOT.IMCAI. GAZETTE TAIILI. I I-KHRIAKV Sample no. Soil strata (inches) Xajiic of farm or farm owner Location I'ir. entaRC of \olatile matter Percentage of sulphur Percentage of phos- phorus I A. .. 0-() Wah-Bec-Mce-Mcc farm Michigan 2 .076 0.0158 0.0360 I ]^ . . 7-U Wali-Bcc-Mee-Mee farm Micliigan 2..U1 0.0157 0330 I C. . . 15-24 Wah-Bec-Mcc-Mcc farm Michigan 2.bh2 0.0216 - 0305 2 A... o-() Wah-Bee-Mee-.Mee farm Michigan 4 . C)88 . 0486 0.051 8 2 B . . . 7-14 \Vali-Bee-,Mce-Mef farm Michigan 4.4>Si 0405 0.0561 3 A... 0-0 Wah-Bec-Mce-Mec farm Michigan 2.863 0.0183 . 0390 3R... 7-14 Wah-Bce-Mce-Mce farm Micliigan 2.522 0159 0.0324 f Not 4 A... o-() \\ah-Bce-i\Ice ;\Iee farm Micliigan 4.802 . 036 1 ■j deter- [ mined Not deter- 4B... 7^14 Wah-Iii'o-Mee-Mee farm Michigan 3-754 0.0263 [ mined 5 A... o-() Wah-Bee-Moc-Arce farm Michigan 4 ■ 3 1 1 0.0319 0.0514 5B... 7-14 Wah-Bee-Mec-Mce farm Michigan 3.822 . 0283 046S 5C... 15-24 Wah-Bec-Mee-Mee farm Michigan 3-462 0.0177 • 0305 6 A... 0-6 KvtTctt's farm Ohio i'-^i'i 0.0232 0.0788 6 B . . . 7-14 Everett's farm Ohio 2 . 466 0.0140 . 04 11 7 A... o-() Arnold's farm Ohio 4-642 . 0334 0.0771 7B... 7- Arnold's farm Ohio 2.984 O.OT95 0.0423 8A. .. 0-6 JacoI)_\'s farm Ohio 5.228 0.0281 0.0582 SB... 7- Jacohy's farm Ohio 3.14s 0.0050 0.0326 A . . . o-() Jacoln-'s farm Ohio 14-327 . ogo5 . 0939 gB... 1~ Jacoby's farm Ohio 5 ■ 969 0.0194 034,5 loA. .. 0-6 WajJ^cr's farm Wisconsin 8. 116 0.0351 0.0744 loB. .. 7~ Wafer's farm Wisconsin 6.954 0.0202 . 0649 II A. .. 0-6 Wager's farm Wisconsin 6 . 836 0.0245 0.0795 iiB. .. 7- \\'af:;er's farm Wisconsin 4 043 0.0124 0.0457 12 o-() Ross's farm Indiana 5-75« 0.0172 0.1054 13 0-6 Carr's farm Indiana 4.721 0.0165 0.0628 14 0-0 Reich's farm Indiana 4 -075 00118 . 0490 15 0-6 Bentley's farm (cropped soil) Indiana 4 8o() 0155 0.0566 16 0-6 Bentley's farm ('\irKin soil) Indiana 5 24Q 0233 0.0564 17 0-6 Barnett's farm Indiana 4.462 0.0183 0.0492 18 0-6 McCulloch's farm Indiana 4.807 00155 0.0578 19 0-6 Adina farm Kentucky 7.024 0.0258 0. 1897 20 0-6 Adina farm Kentucky 4.526 0.0232 0.079Q 21 0-6 Adina farm Kentucky 7 ■ 496 0.0131 0. 1636 22 0-6 Adina farni Kentucky 4.884 0.0122 0. 1298 23 0-6 Adina farm Kentucky 4318 0.0206 . 0768 24 0-6 Marshall's farm Kentucky 5-517 0.0264 0.1377 25 o-() Downinj^'s farm Kentucky 5.466 0.0159 0.0977 26 0-6 Downing's farm Kentucky 5-229 0.0236 0.1765 27 a-6 Downinf^'s farm Kentucky- 5 327 0.0153 0.1370 28.... 0-6 ( ientry and Curry's farm Kentucky ().02I 0.0245 0.2355 29 0-6 Scott's farm Kentucky 5.088 0.0235 . 1 500 30 0-6 Sharp's farm Kcntuck\' 6 . 540 0.0161 0. 1779 31 0-6 Moore's farm Kentucky 4- 723 0.0253 0. 1007 32 0-6 l-"o\vler's farm Kentucky 5-051 0.0250 0. 1727 ii 0-6 Watt's farm Kentucky 5-836 0.0163 0. 1306 34-.. 0-6 Tuomey's farm Kentucky 11 . I 05 0313 - 3407 1922] WOODARD—SOIL FERTILITY 95 soil samples are also quite similar in texture. Here again we lincl a high sulphur content with a high organic matter content, and a low sulphur content with a low organic matter content. When we compare different soil types or samples from the same type but from fields which have been cropped differently, however, there is Httle evidence of any relation. Samples 7 B and 9 B have approxi- mately the same sulphur content, yet the volatile matter in the latter is twice that in the former. Both these samples are sub- soils from Ohio, and were taken from fields that were not far apart, but 7 B is on upland silt loam while 9 B is a muck soil. Again, the cropped soil (no. 15) and the virgin soil (no. 16) from Bentley's farm, Indiana, differ only slightly in volatile matter, but dift'er widely in sulphur content. Gentry and Curry's soil (no. 28) has shghtly less \-olatile matter than Sharp's soil (no. 30), but con- siderably more sulphur. Sample 10 A from Wager's farm in Wis- consin is a fine sandy loam soil with very little clay but a large amount of organic matter, as may be recognized by its black color, yet it contains considerably less sulphur than sample 2 A from the Wah-Bee-Mee-Mee farm in Michigan, which is also a sandy loam soil, containing considerable coarse sand with sulScient organic matter to give a black color. It seems, then, that from the sulphur standpoint, as well as the nitrogen standpoint, the character of the organic matter is of more importance than the amount. Sulphur, like nitrogen, is mainly present in the proteins, so that a small amount of high protein organic matter, such as one would obtain by plowing under leg- umes, would Ije more valuable than a larger quantity of organic matter from wheat or oat straw or cornstalks. It seems probable also that the proteins are more readily decomposed than the non- protein organic matter, so that the sulphur and nitrogen would be oxidized more rapidly than the carbon, and the sulphur and nitrogen content might become quite low when there was still a consider- able amount of carbonaceous organic matter in the soil. In all the samples analyzed, the sulphur content was less than the phosphorus content. One of the samples from Ohio which was taken in a low wet place was a muck, very high in organic matter. This soil had nearly as much sulphur as phosphorus in 96 BOTAXICAL a.l/.KTTE [fkhkiary the surfaci' sdII (no. c) A), but the subsoil (no. q H) liad onI_\' a little more than half as much sulphur as j)hosi)lu)rus. 'I'hc dilTerence between the sulphur and ])h()si)horus contents in one of the Mich- igan soils was not great. The surface soil (no. 2 A) contained 0.0486 per cent sulphur and 0.0518 per cent phosphorus, while the subsoil (no. 2B) contained 0.0405 per cent sul]:)hur and 0.0561 j)er cent i)hosphorus. All the other samples were much higher in j)hos- ]")horus than in sulj)hur. 'Hie dilYerence was very great in one of the Indiana soils, which had oxer six times as much phosphorus as sulpliur, and in the Kentucky soils, in most of which the j)h()spho- rus content was from live to eleven times as much as the sulphur. In two of the Kentucky soils the phosphorus content was only three times as much as the sulphur, and in one only four times as much. The Michigan soils, sam])les 15, were taken on the Wah-Bee- ]\Iee-]\Iee farm at White Pigeon, ^Michigan. Samples i and 5 were samj^led to three dei)ths and all the others to two de])ths. These soils are allu\"ial sandy loams, \arying from light brown to dark brown on the surface and grading into a \ellow sandy subsoil containing some gravel. The light colored samples contained more sand in both surface and subsoil and were lower in \-olatile matter, sulphur, and phosphorus, than the darker colored ones. All were low in both suli)hur and j)hosj)horus, but the sul])hur is lower than phosi)horus in all the samples. With the exception of sample i, the suli)hur was always lower in the subsoil than in the surface soil. The Ohio soils. sami:)]es 6-q, were taken near Copley, Ohio. Xos. 6, 7. and 8 are upland silt loams containing some sand. The surface soil is a yellow brown grading into a uniformly light yellow- subsoil, w^hich indicates good underdrainage as well as good sur- face drainage. These soils apparently belong to the type mapped as the Wooster silt loam. The sulphur content was low in both surface and subsoil, while the jihosphorus content was fairly good in the surface but low in the subsoil. In every sample the sub- soil was lower in \"olatile matter, suli)hur, and i)hosphorus than the corresponding surface soil. Sample 9 is poorly drained, and the surface soil has a large amount of organic matter with some silt, sand, and a little clay. 1922] WOODARDSOIL FERTILITY 97 The subsoil has much less organic matter, but the proportion of its other constituents is about the same as in the surface. The surface soil is very high in volatile matter, sulphur, and phosphorus, while the subsoil is very low in both sulphur and phosphorus. The Wisconsin soils, samples lo and ii, are from near Beloit, Wisconsin. They are fine sandy loams, dark brown on the surface and a hghter brown in the subsoil. In both samples the volatile matter, sulphur, and phosphorus are higher in the surface soil than in the subsoil. The sulphur content is low in both surface soil and subsoil in both samples, but the phosphorus is good in the surface soil of both samples, fair in the subsoil of sample lo, and poor in the subsoil of sample ii. Both sulphur and phosphorus are lower in the subsoil than the surface soil in both samples. The Indiana soil samples (nos. 12-18) were taken near Charles- town, Clark County, Indiana. This region is underlain by lime- stone rock, but the rock has been covered by a thick layer of windblown material, from which most of the soils were formed. All the soils sampled were fonned from this windblown material except no. 12, which was taken on the bluft" of a small stream where there was considerable erosion. It seems that the erosion has removed the greater part of the windblown material, and to a large extent the soil is formed from the underlying limestone. This is probably the reason why this sample resembles in general appearance and in chemical composition the Kentucky soils rather than the adjacent soils from the windblown material or loess. Sample 12 has a light brown silt loam surface soil grading into a reddish yellow subsoil. Like the other Indiana soils, the volatile matter and sulphur are low, but the phosphorus is high Hke most of the Kentucky soils. The loessal soils include two types, the one with good natural underdrainage and the other with poor drainage. The fomier, which includes samples 15-18, is a yellow gray silt loam in the sur- face soil and a yellow silt loam in the subsoil. The latter, which includes samples 13 and 14, has a gray or slightly yellowish gray silt loam surface soil underlam by a gray or gray and yellow mottled silt loam subsoil. Both are poorly drained, but sample 13 is more nearly level and has more gray color in both surface and subsoil. All the samples from both types are low in volatile matter, sulphur. gS BOT.WfCM. GA/.ETTE [February and ]-)hos])horiis. Sami)k's 15 and 16 were taken a few rods apart, the fonncr from a fii'ld \vhi\'h had been in alfalfa for several years, and the latter ixorw vir contains iron concretions. \o. 31 is known locally as white oak land, and both are recognized as poor soils. All the other samples are light brown except no. 34, which is a grayish brown. All the Kentucky soils arc low in volatile matter except the clay loams, in which i)art of the volatile matter is probabl\' water of combination. All are low in sulphur, no. 34 being the only one above 0.03 per cent. This sample is from the Trenton formation and contains many un- weathered fragments of limestone. It is possible that the sul- phur content as well as the phosj)horus content of the Trenton limestone ma}- be higher than in other fonnations. No. 34 con- tains 0.3407 per cent of phosjihorus, which is ele\'en times as great as the sulphur content. 'J'his is much higher than an}' of the others, but all the others are high in phosphorus. Relation between amounts of sulphur and phosphorus removed by crops and sulphur and phosphorus contents of SOILS. — A better idea of the suppl}- of sulphur and phosphorus in the soil can be obtained if the pounds ])er acre of these elements found in the surface soil is com])ared with the amounts removed by some of our commim crops. Table II gives the amounts of sulphur 1922] woo DA RD—SOIL FER TILI T Y 99 and phosphorus removed by some of the common crops. The yields per acre and the amounts of phosphorus removed by these yields are taken from Hopkins and Pettit's (34) table, while the amounts of sulphur removed are computed from Hart and Peterson's analyses. As pointed out by Hopkins and Pettit (34), these yields are exceptionally large, but they have been obtained by some farmers, and others may obtain them under proper systems of farming. If, however, smaller yields are removed, it will not prevent soil deple- tion, but will only delay soil exhaustion if the elements removed TABLE II Pounds per acre removed by farm crops Crop Pounds per acre removed ANNUALLY \IELD PER ACRE Sulphur Phosphorus 100 bushels 7.8 17,0 100 bushels 5-8 1 1 .0 50 bushels 51 12.0 3 tons II. 4 9.0 4 tons 130 20.0 8 tons 46.0 30.0 300 bushels 24.7 13.0 Corn, grain. . . Oats, grain . . . Wheat, grain . Timothy, hay. Clover, hay . . Alfalfa, hay. . Potatoes are not returned in some form. In actual practice, failure to return to the soil the elements of plant food which are removed in the crops will result in a gradual decrease in yields, so that the amounts of plant food removed will gradually become less. It is impossible to determine the time when complete exhaustion will take place, but a comparison of the amounts of plant food removed by large crops with the amounts present in the soil will emphasize the importance of renewing the supply in the soil before the soil supply is reduced below that necessary for satisfactory crop yields. Table III gives the pounds per acre of sulphur and phosphorus in the surface soils analyzed and the number of years' supply of each for several common fami crops, if maximum crops are removed, such as are given in table II. Table III shows that all the soils are too low in sulphur to grow alfalfa for 40 years, while 22 of them have phosphorus enough to BOTAMCAL GA/.ETTE [FEBRrARY <::^ro\v alfalfa 40 years or longer, i")rovi(le(l, of course, none of these elements is added in any way and none removed except in the crops. Sample 9 A, which has the highest sulphur content, has sulphur TABLE III Pounds pkr acrk of sulphur and piiosi'iiorus and xrMHi:R or ykars' supply FOR VARIOUS CROPS IF MAXIMUM CROPS Ml Soil no. 1 A 2 A 3 A 4 A 5 A 6 A 7 A SA qA 10 A 11 A 12 . . 13- ■ 14. . IS- • 16. . 17. . 18. . 19.. 20. . 21 . . 22. . 23- • 24. . 25 • • 26. . 27 . . 28. . 29. . 30. . 3I-- 32.. ? 072 366 638 464 668 562 1810 702 4QO 344 330 236 310 466 566 310 5i(> 464 262 244 412 528 318 472 306 490 470 322 506 500 326 626 Xo. of years' supply for Corn Wheat Timo- thy 12.S igl 47 72 03 142 82 125 60 91 86 13 1 72 1 10 ^?,2 355 go 138 63 q6 44 (,7 42 (>5 30 46 40 61 60 91 47 72 40 61 ()6 lOI Oo 01 34 51 31 48 53 88 68 104 41 64 61 93 39 50 63 96 60 02 41 63 6.S 99 64 98 42 '•4 80 I 2 2 28 85 30 63 41 00 50 159 62 43 30 29 2 I 28 41 ?,2 28 45 41 3f> 46 28 41 27 43 41 28 44 44 29 Clover 24 75 28 5^' 49 36 5' 43 139 54 38 26 25 18 24 3f^ 28 24 40 3(> 20 19 32 41 24 36 24 38 36 25 39 38 -S 48 Alfalfa 14 Phosphorus -do , 1056 780 1028 1576 1542 1 164 1878 14S8 1590 2108 1256 980 I 132 I 128 984 II 56 3794 1598 3272 2596 1536 2754 1954 3530 2740 4710 5000 3558 2014 3454 2612 6814 No. of years' supply for Corn 42 61 46 60 93 91 68 1 10 88 94 124 74 58 67 66 58 68 223 94 192 153 90 162 "5 208 161 277 176 209 118 203 154 401 Wheat 60 86 63 86 131 129 97 X56 124 ^^?, 105 82 94 94 82 96 316 133 273 216 128 230 163 294 228 393 250 296 168 288 218 568 Timo- thy 80 115 83 114 175 171 129 209 165 177 234 139 109 126 125 109 128 422 / / 364 288 171 306 216 392 304 523 395 224 384 290 757 Clover 36 52 39 79 77 58 94 74 80 "05 ''3 49 49 58 190 80 164 130 77 138 98 177 137 236 150 178 lOI 173 131 341 Alfalfa 29 44 43 32 52 41 44 59 35 27 31 31 27 :?,2 105 44 91 72 43 77 54 98 76 131 83 99 56 96 73 189 enough for 39 years of altalfa and phosphorus enough for 52 years of alfalfa. Only one other soil, no. 2 A, had enough sulphur for 20 }'ears of alfalfa, while three soils, nos. 19, 28, and 34, have enough 1922] WOODARD—SOIL FERTILITY lOI phosphorus for loo or more years of ah"alfa. No. 34 has phosphorus enough to grow alfalfa 189 years, but sulphur enough for only 14 years. The phosphorus content of no. 28 is sufficient to grow alfalfa for 131 years, but the same crop would deplete the sulphur in II years. All these soils have sufficient phosphorus to grow maximum yields of alfalfa for 20 years or longer, while all but two would be depleted of sulphur in less than 20 years. Of the other crops mentioned, corn, wheat, and clover remove smaller amounts of sulphur than phosphorus; while timothy, like alfalfa, removes more sulphur than phosphorus. Timothy, how- ever, removes only about one-fourth as much sulphur, and one- fourth as much phosphorus as alfalfa, so that the supply of each would last correspondingly longer, yet soil 9 A is the only one that carries sufficient sulphur for 100 crops of timothy. Soil 9 A has sulphur enough to grow timothy 159 years, clover 139 years, corn 232 years, and wheat 355 years. No. 34 has phosphorus enough for 401 corn crops, 568 wheat crops, and 341 clover crops; yet the sulphur would be depleted by 80 corn crops, 122 wheat crops, or 48 clover crops. The lowest phosphorus content is in soil i A, a sandy loam soil, which has 720 pounds of phosphorus in the surface 7 inches of soil. The phosphorus in this soil would be depleted by growing corn 42 years, wheat 60 years, timothy 80 years, clover 36 years, or alfalfa 20 years. In the same soil the sulphur would be removed by 40 years of corn, 62 of wheat, 28 of timothy, 24 of clover, or 7 of alfalfa. Table III shows the importance of both sulphur and phosphorus if maximum crops of legumes, particularly alfalfa, are to be grown. It also shows that, in most soils, sulphur is more hkely to be defi- cient than phosphorus. It does not take into account the leaching of these elements from the soil, which is practically nil in the case of phosphorus and very high in the case of sulphur; nor the supply in the rain water, which is nil in the case of phosphorus and may be quite high in the case of sulphur near cities in the humid regions. Whether the amount of sulphur lost in the drainage water exceeds that gained in the rain water is still unknown. It is certain that the amount of leaching will vary with the character of the soil, the rainfall, and the character of the plant growth. The amount of 102 BOTAXrCAL GAZETTE [ikhrlary sulphur in the rain water will \ar)- with the rainfall and the near- ness to cities where large amounts of soft coal are used. It is possible that, in some places mider certain conditions, the amount of suli)hur brought down in the rain water will equal or exceed that lost in the drainage, but that in other places and under other con- ditions the loss will exceed the gain. Field ex])eriments are needed to see whether the plants will respond to sulphur fertilization under field conditions. Remarkable responses were obtained b}- Judge Peters, John Binxs, and Edmund Ruffix in the Ivistern United States (Crocker, 15), and have recently been obtained on the Pacific Coast by Reimer and Tart.xr (58) in Oregon, and by Olsox (54) in Washington. To secure further information along this line, coo])erative experiments were conducted on some farms in Indiana and Kentuck}- from which some of the samples reported in table I were taken. COOPERATIVK FIELD EXPERIMEXTS WITH GYl'SUM The field experiments were conducted in cooperation with the fami owners. The fami owners were to apply gyj^sum and rei)ort on the eft'ect on yields, if any. Some of the farmers failed to make any report, and those w'ho did gave no w'eights, so that the results are not as satisfactory- as could be desired. Results reported are as follows. In the Indiana exi^eriments, g}i:)sum was applied to alfalfa, red clover, and tobacco. The only report received was with regard to the tobacco. This tobacco field was on the fami of Mr. Ross, southwest of Charlestown, Indiana. This is the field from which sample 12 was taken, and, as show^n in tables I and III, is low in suli)hur and high in phosphorus. Mr. Ross reports a marked increase in }-ield of tobacco from the use of g}-psum on this field, but gives no ciuantitative data. Gypsum was applied to alfalfa, red clo\er, sweet clover, and tobacco in Mason County, Kentuck}-. The crops were injured so badly by weather conditions, however, that no results were obtained. In Mercer County, Kentucky, gypsum was applied to tobacco, clover, and alfalfa. Of the fanners resi)onding, Mr. Sharp reported no increase in tobacco, while Mr. Fowler reported an increase in 1922] WOO DARD— SOIL FERTILITY 103 the second clover crop, and Mr. Tuomey an increase in alfalfa. Neither of these men weighed the hay, so the results are not quan- titative. Mr. Sharp's field, from which sample 30 was taken, is low in sulphur and high in phosphorus, but it showed evidences of being fanned hard, and was evidently low in nitrogen, which was probably the hmiting element for a non-leguminous crop like tobacco. Mr. Fowler's soil, no. ^2, has 0.0250 per cent sulphur and 0.1727 per cent phosphorus, equivalent to 500 pounds of sul- phur, and 3454 pounds of phosphorus, in the surface soil; so sulphur was probably the limiting element for clover. Mr. Tuomey 's field, sample 34, had 6814 pounds of phosphorus, the highest of the samples analyzed. This sample also contained small fragments of limestone, so that there was an abundance of lime. On the other hand, the sulphur content, 626 pounds, although higher than in many samples, is probably rather low for a plant like alfalfa, which uses such large quantities of sulphur. These results are not conclusive, but it seems probable that sulphur may be a limiting element on some of these soils, and that gypsum is a satisfactory source of supply for this element. More field experiments are necessary in the humid part of the United States, and great care in conducting these experiments is necessary if satisfactory results are to be obtained. Experiments should be conducted through several years to avoid weather conditions, which may be the limiting factor in some years. On some soils drainage is necessary, and no fertilizer treatment will have any eft'ect until this is done. Most soils in the humid part of the United States are acid. A large part of them are so acid that Hming is necessary before any other treatment is effective, especially for leguminous crops. Table I shows a high phosphorus content in some of the soils reported in this paper, but those are exceptional soils. As a general rule soils are deficient in phosphorus, and fanners report increases in crop yields for the use of acid phosphate. It is impos- sible, however, to tell how much of the increase is due to the phos- phorus and how much to the sulphur in the acid phosphate. A comparison of acid phosphate with rock phosphate and gypsum, and with g)^psum alone, and rock phosphate alone would give some valuable results. I04 BOTAXICAL GAZETTE [fkbriary ]\Iany of the Illinois cxjx'rimenl iields include three check plots in each series. These check plots arc all untreated and are only a short distance apart, yet some of them differ widely in croj) yields. It is reasonable to assume that nei- 42 years of corn, 60 of wheat, 80 of timothy, 36 of clover, or 20 of alfalfa. On the other hand, it would take 401 years of corn, 568 of wheat, 757 of timothy, 341 of clover, or 189 of alfaha to remove as much phosphorus as is found in the soil with the highest phosphorus content. 5. On some of the soils tobacco, clover, and alfalfa have been benefited by the use of gypsum. The results, however, are not quantitative. More field experiments are needed and greater care should be taken to eliminate other factors as far as possible. Each treatment should be replicated to reduce the probable error. This investigation was conducted under a research fellowship from the Gypsum Industries Association. The work was perfonned at the University of Chicago in the Hull Botanical Laboratory under the direction of Dr. William Crocker. The author wishes to thank the Gypsum Industries Association for their kindness in furnishing the fellowship and Dr. Crocker for his kind and helpful advice and criticism. Thanks are also due Dr. Frederick Koch for his kind advice and criticism of analytical methods. University of Illinois Urban.a, III. lo6 BOTAMCAL CA/.ETTE [i-kbruary I.ITKRAI TRK (VYVA) 1. Ami.s. J. W., and Riciimoxd, 'J'. K., Ft-rnifiitatioii of manure treated with sulphur and sulphates. Changes in nitrogen and phos[)horus content. Soil Science 4:70-80. 1017. 2. — — ■ — Effect of sulphofication and nitrification on rock phosphate. Soil Science 6:351-364. loiS. 3. BouLL.AXGER, E., and Dugakdix, M., Mechanisme de Taction du soufre. Compt. Rend. Acad. Sci. Paris 155:327-320. iqi2. 4. Br.\di.ky. C. E., The reaction of lime and gypsum on some Oregon soils. Jour. Ind. and Engin. Chem. 2:520-530. loio. 5. Brezealk, J.E.. and Briggs, L.J.. Concentration of potassium in ortho- clase solutions not a measure of its availability to wheat seedlings. Jour. Agric. Res. 20:615-621. 1021. 6. Briggs, L. J., and Brezeale, J. F., .\vailability of potash in certain ortho- clase bearing soils as affected by lime or gypsum. Jour. Agric. Res. 8:21-28. 1917. 7. Brioux, Cii., and C.rERBKT, :\I., L'action fertilisante du soufre. Annales Sci. Agron. 30:305-306. 1913. 8. Brooks, W. P., Alfalfa. Mass. Agric. Exp. Sla. Bull. 154. 1914 (p. 158). 9. , Phosphates in .Massachusetts agriculture, importance, selection, and use. Mass. Agric. Exp. Sta. Bull. 162. 1015. 10. Browx, p. E., and Gwixx, A. R., Effect of sulphur and manure on avail- ability of rock phosphate in soil. Iowa Agric. Exp. Sta. Res. Bull. 43: 373-379. 1017- 11. Browx, p. E., and Kellogg, F2. H., Sulphofication in soils. Iowa Agric. Exp. Sta. Res. Bull. 18:104-110. 1914. 12. Browx, p. E., and W'arxer, H. W., The production of available phosphorus from rock phosphate by composting with sulphur and manure. Soil Science 4: 260-282. ioi7- 13. Browxe, J. D., The field book of manures or the American muck book. 1854 (pp. 68-75). 14. Bruckxer, W. II., American manures. 1872 (p. 65). 15. Crocker, \Vm., History of the use of gypsum as a fertilizer. (Unpub- lished article.) 16. Demolox, M. a., Recherches sur Taction fertilisante du soufre. Compt. Rend. Acad. Sci. Paris 156:725-728. 1913. 17. Duley, F. L., The relation of sulphur to soil productivity. Jour, Amer. Soc. Agron. 8: 154-160. 1016. 18. Dymoxt), T. S., Hughes, F., and Jupe, C. \V. C, The intlucnce of sulphates as manures upon the yield and feeding value of crops. Jour. Agric. Sci. i : 217-229. 1905. 19. Eatox, S. v., Sulphur content of soils and its relation to plant nutrition. (Unpublished article.) i9::2] WOODARD—SOIL FERTILITY 107 20. Ellett, W. B., and Harris, W. G., Cooperative experiments for the composting of phosphate rock and sulphur. Soil Science, 10:315-325. 1920. 21. Feilitzen, H. von, t)ber die Verwendung der Schwefelbliite zur Bekampf- ung des Kartoffelschorfes und als indirekes Diingemittel. Fuhling's Landw. Zeit. 62:239. 1913. 22. Fraps, G. S., The effect of additions on the availability of soil potash, and the preparation of sugar humus. Texas Agric. Exp. Sta. Bull. 190. 1-30, 1916. 23. Fred, E. B., and Hart, E. B., The comparative effect of phosphates and sulphates on soU bacteria. Wis. .^gric. Exp. Sta. Res. Bull. 35. pp. 42-44- 1915- 24. Greaves, J. E., Carter, E. F., and Goldthorpe, H. C, Influence of salts on nitric nitrogen in soUs. Jour. Agric. Res. 16:107-135. 1919. 25. Griffiths, A.B., A treatise on manures. 1889 (pp. 247-248). 26. Hall, A. D., Book of Rothamsted experiments. 1917. 27. Hart, E. B., and Peterson, W. H., Sulphur requirements of farm crops in relation to the soil and air supply. Wis. Agric. Exp. Sta. Res. BuU. no. 14. 1911. 28. , Sulphur requirements of farm crops. Jour. Amer. Chem. Soc. 33:549- 1911- 29. Hart, E. B., and Tottingham, W. E., Relation of sulphur compounds to plant nutrition. Jour, .\gric. Res. 5:233-248. 191 5. 30. Heinrich, R., Concerning the conservation of manure. E.S.R. 5:329, 330. 1893-1894. Abst. from Landw. presse 20:825. 1893. 31. Hilgard, E. W., Soils; their formation, properties, composition, and relation to climate and plant growth. 1906 (p. 43). 32. Hopkins, C. G., Soil fertility and permanent agriculture. 1910 (pp. 39, 1S9). 33. Hopkins, C. G., Hosier, J. G., Pettit, J. H., and Readhimer, J. E., Hardin County soils. 111. Agric. Exp. Sta., Soil Report no. 3. 191 2. 34. Hopkins, C. G., and Pettit, J. H., The fertility in Illinois soils. 111. Agric. Exp. Sta. Bull. no. 123. 1908 (pp. 533-535)- 35. Hunt, Thos. F., Soil fertility. Pa. State Coll. Bull. no. 90. 1909. 36. Lawes, J. B., and Gilbert, J. H., Report on the growth of red clover by different manures. Jour. Roy. Agric. Soc. 21 : 194. i860. 37. LiEBiG, J. VON, Principles of agricultural chemistry. 1855. Transl. by Wm. Gregory, p. 99. 38. Lint, H. C, The influence of sulphur on soil acidity. Jour. Ind. and Engin. Chem. 6:747. 1914. 39. Lipman, C. B., and Gericke, W. F., Does calcium carbonate or calcium sulphate treatment affect the solubility of the soil constituents? Univ. Calif. Publ. Agric. Sci. 3:271-282. 1918. loS BOTAMCM. GAZETTE [fkbrl'ary 40. — ■ — ■ — \ I'lu' sij^nilkaiue of the sulphur in suljjhato of ammonia appUcd to certain soils. Soil Science 5:81-86. IQ18. 41. Lii'M.w. J. (;.. and Jokkk, J. .S., The intluence of initial reaction on the o.xidation of sulphur and the formation of available i)hosphates. Soil Scienci- io:,^-'7-332. i()2o. • 42. Lii'MA.N, j. (;., and McLk.vx, II. ('., Suli)hur-phosphate com|)osts under field conditions. Soil Science 5: 24,-; -2 50. i()i8. 43. Lii'M.v.v, J. (;.. McLkan'. H. C., and Lint, H. C"., Suli)hur o.xidation in soils and its elTect on the availability of mineral phosphates. Soil Science 2 1408 -5 ■58. i()i6. 44. LvDX, T. L., and Bizzkij.. J., Lysimeter experiments. Cornell Univ. .\f;ric. Mx]). Sta. Mem. 12. i<)i8. 45. McCaii., .\. (;., and Smith, .\. M., Effect of manure-sulphur composts upon the availability of the potassium of grecnsands. Jour, .\gric. Res. 19:230-255. 1Q20. 46. McCooL, M. M.. and .Mii.i.ar, C. K., Effect of calcium sulphate on the st)lubility of soils. Jour, .\gric. Res. 19:47-54. iq20. 47. .MacI.xtirk, \V. H., W'ii.i.is, L. (1., and IIoi.di.vg, W. .\., The divergent effects of lime and magnesia upon the conservation of soil sulphur. Soil Science 4:231 237. 1917. 48. .McLkax, H. C, The o.xidation of sulphur by micro-organisms in its rela- tion to the availability of phosphates. Soil Science 5:251-200. 1918. 49. .Mc.MiLi..\R, P. R., Inlluence of gypsum upon the .solubility of potash in soils. Jour. Agric. Res. 14:61-66. 10 1 8. 50. -Marls, M. H., Des transformations que subit le soufre en poudre (lleur de soufre et soufre triture) quand il est repandu sur le sol. Compt. Rend. Acad. Sci. Paris 69:074 -070. i860. 51. Mii.r.KR, II. G., Sulphates affecting plant growth and composition. Jour. .\gric. Res. 17:87-101. i()i(). 52. MoRSK, F. \\'.. and Ci rrv. H. l"... The availability of the soil potash in clay and clay loam soils. X. 11. .\gric. E.xp. Sta. Bull. 142. pp. 40-51. iQog. 53. Xoi.TE, Otto, Uber die Ursache der stickstoffverluste aus Jauche and Stallmist. Landw. \'ers. Stat. 96:309-324. 1920. 54. Oi.so.x, Geo. .'\., Unpublished work of the Chemistry Department, Washing- ton State College. 55. Pi:ti;rso.x. W. H., Forms of sulphur in plant materials and their variation with the soil supply. Jour. .Vnu-r. Chem. Soc. 36:1290-1300. 1914. 56. Pi-KIFFKR, Th., and Bi.axck. Iv, Landw. Vers. Stats. 83:359-383. 1914. 57. PiTZ. \\., F>ffect of elemental sulphur ami calcium sulphate on certain of the higher and lower forms of plant life. Jour, .\gric. Res. 5:771 780. 10 16. 58. Rki.mkr, V. C, and Tartar, II. W, Sulphur as a fertilizer for alfalfa in Southern Oregon. Ore. Agric. l^.xp. Sta. Bull. 163. 1919. 1922] WOODARD—SOIL FERTILITY 109 59. RoBixsoN, \V. O., Inorganic composition of some American soils. U.S. Dept. Agric. Bull. 122. 1914. 60. Robinson, W. O., Steinkonig, L. A., and Fry, W. H., Variation in chemical composition of soils. U.S. Dept. Agric. Bull. 551. 1Q17. 61. ScHREiNER, O., The role of oxidation in soil fertility. U.S. Dept. Agric. Soils Bureau Bull. 56. pp. 30-42. 1909. 62. Shedd, O. ]\I., The sulphur content of some typical Kentucky soils. Ky. Agric. Exp. Sta. Bull. 174. 1913. 63. , Effect of sulphur on different crops and soils. Jour, .\gric. Res. 11:91-103. 1917- 64. Sherbakoff, C. D., Potato scab and sulphur disinfection. Cornell Univ. Agric. Exp. Sta. Bull. 350. 738, 739. 1914. 65. Smith, R. S., Some effects of potassium salts on soils. Cornell Univ. Agric. Exp. Sta. Mem. 35. p. 5S6. 1920. 66. Stewart, J. P., The fertilization of apple orchards. Pa. State Coll. Agric. Exp. Sta. Bull. 153. 1918. 67. Stewart, Robert, Sulphur in relation to soil fertility. 111. Agric. Exp. Sta. Bull. no. 227. 1920. 68. Swanson. C. O., and Miller, R. W., The sulphur content of some typical Kansas soils and the loss of sulphur due to cultivation. Soil Science 3:139-148- IQI7- 69. Tressler, D. K., The solubility of soil potash in various salt solutions. Soil Science 6:237-257. iqiS. 70. Vendelmans, Henry, Manual of Manures. 1916 (p. 142). 71. Vivien, A., Abs. E. S. R. 17:951; from Monit. Sci. 4: ser. 19. no. 2. 773-779- 1905- 72. VooRHEES, E. B., Fertilizers. 191 7- Revised ed., p. 116. 73. Warington, R., Reprint from Jour. Chem. Soc. 47. 1885. LIBRORY OF CONGRESS 000 937 786 4