713 U. S. DEPARTMENT OF AGRICULTURE. OFFICE OF EXPERIMENT STATIONS, I A. C. TRUE, Director. INVESTIGATIONS NUTRITION OF MAN IN THE UNITED STATES. C. F. LANGWORTHY, Ph. D., and R. D. MILNER, Pii. B. Of the Office of Experimeni Statiom^. WASHINGTON: GOVERNMENT PRINTING OFFICE. 1904. LIST OF PUBLICATIONS OF THE OFFICE OF EXPERIMENT STATIONS ON THE FOOD AND NUTRITION OF MAN. E. — For those publications to which a price is aflixoil application should be made to the Super- lent of Documents, Government Printing Office, Washington, I). C, the oflflcer designated by KOTE.- intenden law to sell Government publications. Publications marked with an asterisk (*) are not available for distribution. ♦Charts. Food and Diet. By W. O. Atwater. (Four charts, 26 by 40 inches.) Price per set, unmounted, 75 cents. *Bul. -Jl. Methods and Results of Investigations on the Chemistry and Economy of Food. By W. O. Atwater. Pp. 222. Price, 15 cents. Bui. 2S. (Kevisud edition.) The Chemical Composition of American Food Materials. By W. 0. Atwater and A. P. Bryant. Pp. 87. Price, 5 cents. Bui. 29. Dietary Studies at the University of Tennessee in 1895. By C. E. Wait, with comments by W. ()! Atwater and C. D. Woods. Pp. 45. Price, 5 cents. Bui. 31. Dietary Studies at the University of Missouri in 1895, and Data Relating to Bread and Meat " Con.-i'umption in Missouri. By H. B. Gibson, S. Calvert, and D. W. May, witli comments by W. O. Atwater and C. D. Woods. Pp. 24. Price, 5 cents. *Bul. 32. Dietary Studies at Purdue Univensitv, Lafayette, Ind., in 1895. By W.E.Stone, with com- ments bv W. O. Atwater and C. D. Woods. Pp. 28. Price, 6 cents. Bui. 35. Food and Nutrition Investigations in New Jersey in 1895 and 1890. By E. B. Voorhees. Pp. 40. Price, 5 cents. * Bui. 37. Dietary Studies at the Maine State College in 1895. By W. H. .Jordan. Pp. 57. Price, 5 cents. Bui. 3H. Dietary Studies witli Reference to the Food of the Negro in Alabama in 1.895 and 1S96. Con- ducted with the cooperation of the Tuskegee Normal and Industrial Institute and the Agri- cultural and Mechanical College of Alabama. Reported by W. O. Atwater and C. D. Woods. Pp. 69. Price, 5 cents. Hul. 10. Dietary Studies in New Mexico in 1895. ByA. Goss. Pp.23. Price, 5 cents. Bui. 13. Losses'in Boiling Vegetables and the Composition and Digestibility of Potatoes and Eggs. By. H. Snyder, A. .7. Frisby, and A. P. Bryant. Pp. 31. Price, 5 cents. Bui. 41. Report of Preliminary Investigations on the Metal)olism of Nitrogen and Carbon in the Human Organism with a Respiration Calorimeter of Special Construction. By W. O. Atwater, CD. Woods, and F. G. Benedict. Pp. 64. Price, 5 cents. Bui. 15. A Digest of Metabolism Experiments in which the Balance of Income and Outgo was Determined. By W. O. Atwater and C. F. Laugworthy. Pp. 434. Price,- 25 cents. *Bul. 16. Dietary Studies in New York City in 1895 and 1896. By W. O. Atwater and C. D. Woods. Pp. 117. Price, 10 cents. Bui. .52. Nutrition Investigations in Pittsburg, Pa., 1894-1896. By Isabel Bevier. Pp. 48. Price, 5 cents. Bui. .53. Nutrition Investigations at the University of Tennessee in 1896 and 1897. By C. E. Wait. Pp. 10. Price, 5 cents. *Bul. 54, Nutrition Investigations in New Mexico in 1897. By A. Gos.s. Pp.20. Price, 5 cents. Bui. 65. Dietary Studies in Chicago in 1S95 and 1896. Conducted with the cooperation of Jane Addams and Caroline L. Hunt, of Hull House. Reported by W. O. Atwater and A. P. Bryant. Pp.76. Price, Scents. * Bui. ,56. His'tory and Present Status of Instruction in Cooking in the Public Schools of New York City. "Reported by Mrs. Louise E. Hogan, with an introduction by A. C. True, Ph.D. Pp. 70. Price, 5 cents. Bui. 63. Description of a New Respiration Calorimeter and Experiments on the Con.servation of Energy in the Human Body. By W. O. Atwater and E. B. Rosa. Pp. 94. Price, 10 cents. * Bui. 6C. The Physiological KiTect of Creatin and Creatinin and their Value as Nutrients. By J. W. Mallet. Pp.24. Price, 5 cents. Bui. 67. Studies on Bread and Bread Making. By Harry Snyder and L. A. Voorhees. Pp. 51. Price, 10 cents. Bui. 68. A Description of Some Chinese Vegetable Food Materials and Their Nutritive and Economic Value. By W. C. Blasdale. Pp. 48. Price, 10 cents. B)il. 69. Experiments on the Metabolism of Matter and Energy in the Human Body. By W. O. Atwaterand F. G. Benedict, with the cooperation of A. W. Smithand A. P. Bryant. Pp.112. Price, 10 cents. Bui. 71. Dietary Studies of Negroes in Eastern Virginia in 1897 and 1898. By H. B. Frissell and Isabel Bevier. Pp. 45. Price, 5 cents. Bui. 7.5. Dietary Studies of University Boat Crews. By W. 0. Atwater and A. P. Bryant. Pp. 72. Price, 5 cents. Bui. 84. Nutrition Investigations at the California Agricultural Experiiment Station, 1896-1898. By M. E. Jaffa. Pp. 39. Price, 6 cents. Bui. 85. A Repr)rt of Investigations on the Digestibility and Nutritive Value of Bread. By C. D. Woods and L. H. Merrill. Pp. 51. Price, 5 cents. [Continued on third page of cover.] U. S. DEPARTMENT OF AGRICULTURE, l\.S. OFFICE OF EXPERIMENT STATIONS, A. C. TRUE, Director. 1 INVESTIGATIONS NUTRITION OF MAN IX THE UNITED STATES. BY C. F. LANG WORTHY, Ph. D., and R. D. MILNER, Ph. B., OJ tlie Office of Exjie7'i7nen1 Staticnts. WASHINGTON: GOVEKNMENT PRIXTIXG OFFICE. 19 04-.; 6^^ > ,' 3 3 3 3 OFFICE OF EXPERIMENT STATIONS. A. C. True, Ph. D., Director. E. W. Allen, Ph. D., Assistant Director and Editor of Kvperime)tt Statio)i Record. C. F. L.\X(;woRTHY, Ph. I)., Editor and Expert on Foods and Aiiimal Production. NUTRITION INVESTIG.\TIONS. W. O. Atwater, Ph. D., Chief of Xulrition Invesiif/ations, Middletovn, Conn. C. D. Woods, B. S., Special Agent at Orono, Me. F. G. Benedict, Ph. D., PlHjdological Chemist, Middleiown, Conn. R. D. INIiLNER, Ph. B., Editorial Assistant, MiddJetoicn, Conn. LETTER OF TRANSMITTAL. U. S. Department of Agriculture, Office of Experiment Stations, Washington^ D. 6'., May 25^ 190 Jf.. Sik: I have the honor to transmit herewith and recommend for pub- lication a summary showing- the scope of the cooperative nutrition investigations carried on under the auspices of this Otiice. This includes an account of the origin and development of the investiga- tions and their character, data regarding the collaborators and collab- orating institutions, and also some of the more important results obtained. Respectfully, A. C. True, Dinctor. Hon. James Wilson, Sccreta ry of A f legumes, including ])eans and cowpeas. Studies of the comparative econoniy, digestibility, and nutritive value of fruits and nuts. A convenient and comparatively inexpensive form of bomb calorimeter has been developed and is used in determining the amounts of potential energy in food mate- rials, excretory products, and other substances. Metabolism experiments have l>een carried on with men in the respiration calo- rimeter. The oV)jects of these investigations have lieen: To develop an apparatus and method for the accurate measurement of the income and outgo of the animal organism as expressed in terms of matter and energy; to confirm the belief that the law of the conservation of energy obtains in the living body; and, after such demon- stration of the accuracy of the apparatus and methods and of the action of the law of the conservation of energy, to study some of the more important fundamental laws of nutrition. The fact was early recognized that compilations and summaries of the investiga- tions which have been carried on in Europe and this country were neces.sary. In order to conduct any kind of research most efficiently it is necessary to know what has been accomplished by others in order that the investigator may profit by their results and that an unnecessary duplication of effort may Ije avoided. Furthermore, summaries of previous investigations are of great value in suggesting lines of research and in awakening the interest of investigators by showing past achievements. To this end the current literature of the nutrition of man, w-hich is now quite volumi- nous, is being regularly followed up, and such abstracts and compilations are being made as will promote the intere.st of the investigations. The rapid accumulation of material which must l)e made ready for publication necessitates a large amount of editorial work. The results of the investigations are given in bulletins, some of popular nature and others technical in character. The results of analyses of food materials in the United States have been compiled and printed m a bulletin for popular use, Avhich is revised from time to time as data accumulate. The results of dietary studies also appear in bulletins prepared for popular use. The data of the digestion experiments and those obtained in investi- gations with the respiration calorimeter are given in bulletins more or less technical in character, and intended rather for the scientific student of the subject. Abstracts of the current literature of the subject of food and nutrition appear regularly in the Experiment Station Record. More popular abstracts of some of this work, particu- larly that of American investigators, appear in the series of Farmers' Bulletins, entitled "Experiment Station Work." The correspondence connected with the nutrition investigations has grown to very considerable dimensions. 10 DISTRIBUTION OF THE WORK. The following is a brief snmmaiy, alphabeticalh' bj' States, of the localities in which the inquiries have been prosecuted, the cooperating institutions and investigators, and the nature of the investigations conducted : Alahmna. — Tnskegee Normal and Agricultural InstiUite; Prof. Booker T. Wash- ington. Alabama Polytefhnic Institute and the Alabama Experiment Station, Auburn; Prof. B. B. Ross. Study of the food consumption of the negroes in the "black belt" of Alabama, and of the character and nutritive value of the food materials used by negroes. California. — University of California; Prof. M. E. Jaffa. Cost and nutritive value of California food materials, especially fruits and nuts; dietary studies of infants, athletes, professional men, fruitarians, and Chinese; digestion and nitrogen meta- bolism experiments with an infant and with fruitarians. In addition, INIr. W. C. Blasdale, instructor in chemistry at the university, made a detailed study of the nutritive and economic value of Chinese vegetal)le food materials found in the markets of San Francisco. Comiedicut. — Wesleyan University and Storrs Experiment Station; Prof. W. O. Atwater, Prof. F. G. Benedict, and associates. Cost and nutritive value of various food materials; dietary studies; digestion and nitrogen metabolism experiments; development of bomb calorimeter and respiration calorimeter, and of methods of using these in investigations of the metabolism of matter and energy in the human body. The work of the office of Professor Atwater, who is chief of the nutrition investi- gations, has also included the planning and direct supervision of the cooperative inves- tigations in different parts of the country, the compilation of the results of nutrition investigations in the United States and foreign countries, editorial work in the prepa- ration of the reports of the eooperators for publication, and a large correspondence relating to the investigations. Georgia. — University of Georgia; Dr. H. C. White. Dietary studies of t-ollege boarding, clubs, and of families in the mountain regions of Georgia. Illinois. — Hull House, Chicago; ISIi.ss Jane Addams and ISIiss Caroline Hunt; and Lewis Institute, Chicago; Prof. G. N. Carman. Dietary studies. ]Mrs. Ellen H. Richards and Mi.ss Amelia Shapleigh also carried on dietary studies with the cooperation of Hull House. The data obtained were eventually submitted to the Department of Agriculture for calculation and publication. University of Illinois; Prof. H. S. Grindley. Cost and nutritive value of food materials; dietary studies: special investigations on meat, including losses in different modes of cooking, and digestibility of meats cooked in different ways. Prof. Isabel Bevier and Miss Elizabeth Sj)rague. Studies in cooking meat. • Indiana. — Purdue University; Prof. W. E. Stone. Dietary studies. Maine. — University of Maine and ]\Iaine p]xi)eriment Station; Prof. W. H. Jordan, Prof. C. D. AVoods, and L. H. Merrill. Studies of the nutritive value of a number of food materials; feeding experiments to determine the value oi milk in the diet; and dietary studies and digestion experiments, especially with lumbermen in the Maine woods. Experiments on the digestibility and nutritive value of bread made from different grades of flour; study of experimental methods, particularly means of separation of feces in digestion experiments and determination of metabolic nitrogen in feces. Maniknah — Mrs. Mary Hinnian Abel cooperated with the D('i)artiuent in the l)rei)aration of summaries of data regarding the nutritive value and ]>]ace in theMiet of sugar and of leguminous vegetables, and in connection with this work made a number of cooking experiments. 11 ^fassac)^vselt.t. — School of Housekeeping, Boston; Miss Lydia Southard, Miss Susannah Usher, and INIiss Bertlia M. Terrill carried on dietary studies, in a number of which the attempt was made to regulate»the cost of the food and at the same time make it compare with commonly accepted dietary standards. The results of these investigations were submitted to the Department for calculation and publication. Harvard University, Cambridge; Dr. E. A. Darling. Dietary studies of athletes. Prof. C. R. Sanger and Mr. Edward INIallinckrodt, jr. Dietary studies of students. Springfield Bible Normal College; Miss Bertha M. Terrill. Dietary study. In this investigation the attempt was made to regulate the cost of the daily food and at the same time make it correspond with the connnonly accepted dietary standards. The results were submitted to the Department for calculation and publication. Minnesota. — University of Minnesota and Minnesota Experiment Station; Prof. Harry Snyder. Study of losses in cooking vegetables and in making bread; special studies of cereal products, including relative nutritive value of milling products of wheat, and digestibility and nutritive value of bread made from different grades of hard and soft wheat flours. A considerable part of the latter investigation is carried on coordinately with similar work by Prof. C. D. Woods at the Maine Experiment Station. Missouri — University of Missouri; Prof. H. B. Gibson. Dietary studies; inve.sti- gation of the relative consumption of different kinds of meat and liread. New Jersey. — New Jersey Experiment Station; Prof. E. B. Voorhees and Mr. L. A. Voorhees. Dietary study; studies of the cost and comijosition of milk and of bread, of the relative cost of bread and the raw ingredients from which it was made, and of the losses in baking bread. Neiv Mexico. — New Mexico College of Agriculture and Mechanic Arts and New Mexico Experiment Station; Prof. Arthur Goss. Sturmo»/.— Vermont Experiment Station; Prof. J. L. Hills. Dietary studies of farmers' families. 12 Virginia. — Hampton Normal and Agricultural Institute; Dr. H. B. Frissell. Dietary studies of negroes. Under special authorization Miss Isabel Bevier also made similar studies in another part of the State. State University; Prof. J. W. ^Mallett. Studies uf the physiological effects of meat bases, i. e., creatin and creatinin. Washington, D. C. — Besides the general supervision vi the plans and expenditures, the work of the Washington office in relation to the nutrition investigations has included the preparation of popular bulletins; the compilation of technical summa- ries of investigations; editorial work in perfecting the details of reports of investiga- tions; collection of bibliographical data; the abstracting of the literature of nutrition, partly for pul)lication in the Experiment Station Record; the conducting of a large correspondence growing out of nutrition investigations, and the distribution of pub- lications on this subject. This w^ork is in charge of Dr. C. F. Langworthy. Cooperating with the U. S. Department of the Interior a series of studies of food consumption has been conchicted in the Government Hospital for the Insane at Washington, D. C. The results obtained were interesting and valuable, and are being utilized to make the diet, which was found to be ample and of good quality, conform to theoretical requirements. This cooperative investigation was begun by Dr. A. B. Richardson and was continued by his successor. Dr. W. A. White, the experimental work having been carried on by Mr. H. A. Pratt. Mention should also be made here of other inve.stioations which, while not strictl}' a part of the cooperative inquirv, are yet .so closely related to it that they may be included in this summary: On behalf of the New York State Commission in Lunacy an extended series of studies of the food consumption of the New York hospitals for the insane was car- ried on under the supervision of Professor Atwater. A study of the food consumption at the Elmira Reformatory, in New York State, was also made under the supervision of Professor Atwater. A sum of money has been granted by the Carnegie Institute to Professor Atwater for the development of accessory apparatus and methods for determination of oxygen in connection with the experiments in the respiration calorimeter, and for investiga- tions of the income and outgo of oxygen in the animal organism. The apparatus has been completed and found to be very accurate (see p. 16). SOME RESULTS OF NUTRITION INVESTIGATIONS. Among the more important results obtained in these investiofations are tho.se from studies of the composition of food material, from dietary studies, digestion experiments, and investigations with the respiratioji calormieter. COMPOSITION OF FOOD MATERIALS. One result of the investigations of food and nutrition in the United States, including botii the work done as a part of the cooperative incjuiiy and that carried on in other connections, is that we have now a tolerably clear idea of the composition and nutritive values of our ordinal V American food materials. In the latest revised edition of a U. S. Dept. of Agr., Office of Expt. Stationb, 713. Plate II. PiilcsZible nutrients n_ _j „ J. Car-bo- MinerctV i^aicin Fats byoU-octct 7na.Ue.rs Indiy^-iAtibU nutncnti Non mitnents. Water Kefu.se. Composition of Food Materials. Nutritive Ingredients, Refuse, and Fuel Value. U. S, Dept. cf Agr., Office of Expt- Stations, 713. Pla-e mi. 18 bulletin" puhlishcd in 1903. in wliich these results are compiled, the total number of analyses included was 4,063. Of these, 1,618 were of animal foods, 2,375 of veg-etable foods, and TO of unclassified foods. At the present time the number of analyses thus compiled is more than 4,5(H) and is still rapidly increasing-. The}' are now derived more largely from other sources than the cooperative nutrition investi- gations, because in connection with the latter they are made onh' when necessar}', as in digestion and metabolism experiments. They are no longer made simplj' to increase the amount of such data available. Plate II shows in graphic form the composition and fuel value of some of the more common food materials. DIETARY STUDIES. The number of studies of the actual food consumption of people of dirt'erent classes that have formed part of the cooperative inquiry in ditferent parts of the United States is now over 500. These include studies of families of day laborers, farmers, mechanics, and men in professional life; of peoj^le in congested districts of the slums of New York and Chicago, and poor families in other cities; of negroes in the South; of Spaniards in the extreme Southwest; and of Chinese and fruitarians on the Pacific coast. In a large number of these studies actual analyses were made of food materials and of waste. In addition to the above, which were made entirely as a part of the cooperative nutrition investigations, nearly half as many studies of a similar nature not directly related to the cooperative inquiry have also l^een completed. The latter include studies in private families, in boarding- houses and clubs of men and women in colleges, in hospitals for the insane, and in other institutions. The total number of pei'sons — men, women, and children — included in all these studies is iK)t far from 15,000. Results of some of the more important dietary studies thus far made are sunnuarized in the following table. These are typical of the studies already published. The data of a consideralile iuunl>er of other studies are now being prepared for publication. «U. S. Dept. Agr., Office of Experiment Stations Bui. 28: The Chemical Composi- tion of American Food Materials. Revised edition, 1903. 14 Summarized results of dktanj studies in the United States [Quantities per man per day.] PERSONS WITH ACTIVE WORK. Rowing clubs in New England Bicyclists in New York Football teams in Connecticut and Cali- fornia PERSONS WITH ORDINARY WORK. Farmers' families Mechanics' families La) hirers' families in large cities Lahiirers' families in more comfortable circumstances PROFESSIONAL MEN. Lawyers, teachers, etc. College clubs MEN WITH LITTLE OR NO EXERCISE. Men in respiration calorimeter PERSONS IN DESTITUTE CIRCCMSTANCES. Poor families in New York City Laborers' families in Pittsburg", Pa. MISCELLANEOUS. Negro families in Alal>ama Negro families in Virginia Italian families in ('hicago French Canadians in Cliicago Bohemian families in Cliicago Inhiibitantsdf .lava Village, C'olumbian E:xi)osition, 1893 Russian .lews in Chicago Mexican families in New Mexico Chinese dentist in ('alifornia Cliinesc lanndrymeii in California Chinese farm lal)orers in California Fruitarians lUr.TAr.Y STANDARDS. Man with very hard muscular work f Atwater) . . .' Man with hard niuscularwork (Atwater) Man wiih niodiTately active muscular work (Atwater) ...". Man with light to moderate muscular work (Atwater) Man at "seileiitary " or woman with moderately active work {.\twater) Woman at light to uKjderate muscular work (Atwater) Actually eaten. Digestible. Gms. 1.55 186 97 103 101 104 107 62 109 103 lis ll.T 66 137 94 115 135 144 .50 175 150 125 112 100 90 Gms. 177 186 354 130 150 116 125 148 132 1.59 111 1.58 101 19 103 71 113 76 95 102 («) (") («) (") C) 1 Si O d 'S Gms. Gms. i 440 143 651 171 634 208 467 89 402 95 344 93 534 110 i 423 96 459 98 305 103 407 86 308 74 436 57 444 100 391 95 345 109 360 106 254 61 418 126 613 86 289 106 566 124 ■ 640 132 237 43 {«) 161 («) 138 (") 115 («) 103 ; (") 92 («) 83 168 177 336 124 143 110 119 141 125 151 105 150 96 18 98 67 107 72 90 92 (") (") («) («) («) Gins. I Cals. 427 ' 3,955 631 5, 005 453 3,415 390 I 3,355 334 2,810 518 3, 925 410 3, 220 445 3,580 296 2, 380 395 299 423 342 379 335 349 246 405 595 281 549 621 («) (") («) («) 2, 845 2,400 3,165 3, 625 2, 965 3. 260 2,800 1,4.50 3, 135 3, 460 2, 620 3, 480 3,980 2, 055 5, .500 4,1.50 3,400 3, 050 2, 700 2, 4.50 6.6 6 8.2 7.5 6.3 4.5 6.9 6.8 12.4 6.8 6.5 6. 2 5.3 4.7 5 8.7 4.9 5.7 6.2 10 7.2 6.2 0.2 6.1 6.1 6.1 " Fats ai;a.sed upon the results of diovstion experiments. The 15 fuel value of the diet — i. c, the amount of energy actually available to the body — has been calculated by the use of the most recent factors for fuel value of nutrients. The table also shows dietary standards that have been proposed on the basis of the data obtained in dietary studies, digestion experiments, and investigations with the respiration calorimeter. From the results of these investigations it is apparent that varia- tions in diet are in part such as naturally follow differences in the actual food supply; but the}' are also intluenced to some extent by race habits, and to a still larger extent by the amount of muscular work performed and by the material circumstances of the consumer, including espe- cially his income. One of the most important ways in which practical application may be made of the results of these studies to the benetit of large numbers of people is in teaching the relative nutritive value of difl'erent food materials and their pecuniar}^ economy. Much is already being done in this direction in different parts of the United States. The proper nourishment of the inmates of institutions where large num))ers must be fed, such as schools, reformatories, prisons, and hos- pitals, is a subject that is attracting no little attention at the present time. In many instances dietary studies have been made in schools, college clubs, etc., and the information obtained has been of much use. That such studies have been found to have a practical value, and that the interest in them is widespread, is shown by the fact that a consid- erable number have been undertaken by instructors and others inter- ested, aside from those carried on by the Department of Agriculture. The dietar}" studies were made under widely varying conditions. Plate III, showing Chinese farm laborers at dinner, is an illustration of this. These laborers were one of the groups studied in an investigation of the dietary of Chinese on the Pacific coast. As a result of such studies as those described above, the dietary standards included in the table have been suggested. These are intended to show the actual food requirements of persons under dif- ferent conditions of life and work, and how these requirements may be most economically and efficiently supplied by the available food materials. It is not claimed, however, that the food each da}- should contain exactly the kind and amounts of the different nutrients required by the standards. A slight deficiency one da}^ will be made good l)y an excess the next, the body serving as a storehouse for reserve material. Experience has, however, shown that the bodj' is best nourished when through long periods the food approximates the requirements of the so-called standards. Individual requirements and individual peculiarities will always affect the choice of foods.. The exact knowledge which comes with such researches is showing how the diet of largo classes of our population ma}' be materially improved while often its cost may be considerably reduced. 16 DIGESTION EXPERIMENTS. Nearly 600 digestion experiments, mosth^ with men but a few with women and children, have been made in connection with the nutrition investigations of the Department. In connection with nearly half of these the income and outgo of nitrogen has been determined, thus making them nitrogen metabolism experiments also. The results of these digestion experiments show the digestibili't}- of various sorts of mixed diet and of individual food materials. Taking these results into account, together with the results of analyses, we are able to prepare tables showing the average quantities of digestible nutrients in a large numl)er of the food materials in most common use. METABOLISM EXPERIMENTS WITH THE RESPIRATION CALO- RIMETER. From the more purelv scientific standpoint, the interest of this cooperative nutrition inquiry culminates in the experiments with the respiration calorimeter. These have for their object the stud}' of the transformation of matter and energy in the living organism. In other words, they represent an inquiry into the most fundamental and most important laws of nutrition. The apparatus used for the purpose, known as the Atwater-Rosa respiration calorimeter, wdiich has been developed in connection with these investigations, suffices for the accurate measurement of the income and outgo of all chemical elements except oxygen, of the potential energy of food, of unoxidized excre- tory products, of body material gained or lost, and of the kinetic energy given otf from the body in the forms of heat and external muscular work. Accessory apparatus and a method for the direct determination of income and outgo of oxygen have very recenth' been completed by Professors Atwater and Benedict, and it is possible with the calorimeter as thus modified to determine directly all the elements of income and outgo and in addition to study the respiratory quotient which is known to be a very delicate index of the processes going on in the body. Plate IV gives ageneral view of the respiration calorim- eter, and Plate V a ground plan of the cidorinieter laboratory. One very important result thus far obtained by the use of this apparatus is what amounts practically to a demonstration that the law of the conservation of energy applies to the living organism. The proof that this law obtains in the inorganic world was one of the great scientific achievements of the last century. It has, of course, been assumed and very generally believed that it nmst also apply in the organic world — in the living being; but a complete and satisfactory demonstration has not hitherto been made, although an approximate proof was found in a iuim})er of European exp(>ri- ments with dogs. As the outcome of 04 experiments by Professors U. S. Dept. of Agr., Of ice of Expt. Stations, 713. Plate IV. U. S. Dept. of Agr., Office of Expt. Stations, 713. Plate V. ^\^ 1^ nH O -< z. < z 17 Atwater and Benedict, and their associates, at Wesleyan University, made with (> different men, covering- an aggregate of one hundred and eighty-four days, the ratio of the potential energy of the material oxidized in the bod}- to the energy given otf from the body in the forms of heat and muscular work, as measured by the respiration calorimeter, is as 1,000 to 99!>. The same ratio of 1,000 to 999 was found in the average of a large number of experiments made to test the accuracy of the apparatus. Closer agreement than this could not be looked for. The practical usefulness of this apparatus and method of inquiry will be realized more clearly when we consider that it gives us a means for measuring more exactl}' than has hitherto been possible, the trans- formations of matter and energy which take place in the bodies of different persons with different kinds and amounts of food or in fast- ing, and under widely varying conditions of muscular and mental activity. The experiments furnish a mass of data needed for various sciences of physiolog}^ and hygiene. These data have to do with food, drink, and excretory products and with the metabolic processes and changes of bod}^ tissue as they are influenced by work, sleep, diet, temperature, and other circumstances. They show the nutritive values of food and the demands of the body in health and disease. Incidentally the}^ throw valuable light on problems of ventilation and numerous other questions of hygiene. They supply the foundations of the doctrine of nutrition and belong to the highest order of scien- tific research. FACTORS FOR DIGESTIBILITY AND FUEL VALUE OF NUTRIENTS. The data obtained in the nutrition investigations ma}' be used in deducing certain factors which are useful in various ways to students and investigators of similar problems, and to some extent also to those who wish to make practical application of the results. Such factors, for the digestibility and fuel value of the nutrients of ordinar}- mixed diet have been epitomized b}' Professor Atwater in the following table: 31^823— IM- — 2 Factors for digestibility and fuel value of nutrients in mixed diet. Protein. Fat. Carbohydrate i- 5 ^ 1 5 Fuel value per gram. 5 " Si 1 5 Fuel value per gram. pi 3 a be s Fuel value per gram. materials. 5S II 5 £ il Eh 11 .£fa 1 ^^ C ^ ^ Meal nnd fish Pa- cent. 43 6 Per cent. 97 97 97 Calor- ics. 4.27 4.37 Calor- ics. 4.40 4.50 Per cent. \ 60 32 Per cent. 95 95 Cator- 9.03 8.79 ffS. 9.50 9.25 Per cent. Per cent. 98 Calor- ies. 3.82 Cator- P?)- •es. 1 cent. 1 87 3.90 ' S9 Dairy products V> 4.27 j 4.40 ■ 1 93 Animal food (of mixed diet) 61 97 4.27 4.40 92 95 8.93 9.40 5 98 3.82 3. 90 89 Cereals 31 85 78 3.87 3.47 4.55 4.45 r ^ 90 8.37 9.30 55 1 1 21 "is' I 5 98 97 98 98 95 90 4.11 4.07 3.87 4.11 3.99 3.60 4.20 ! 91 Legumes (dried) ... 2 4.20 3.95 4.20 4.20 4 00 83 98 98 5 1 83 85 3.11 3. 36 3.75 91 Fruit 3.95 J «.s Vegetable food (of mixeddiet). 39 So 3.74 4.40 8 90 8.37 9.30 95 97 4.03 4.15 92 Total food (of mixeddiet). 100 92 4.05 4. 40 100 95 8.93 9.40 100 97 4.03 4.15 91 Briefly stated, on an average about 96 per cent of the total organic matter of mixed diet will l)e digested and !)1 per cent of the energy will be available to the bod}- or, in other words, the body rejects about 4 per cent of the nutrients and al)out 9 per cent of the energy supplied by the food. With the exception of .some important European determinations of heats of combustion, the figures given in the above talde are derived from late investigations in the United States. These included over 4,500 analyses of food materials; over 500 dietary .studies; nearly (iOO digestion experiments, mostly with men; several thousand determina- tionsof heatof combustion of food materials and excretor}" products, and CA experiments, covering in the aggregate 184 days, with men in the respiration calorimeter, besides a considerable number of other experi- mental iiKjiiirics, including especially the determinations of the con- stitution of protein compounds in various materials. The results of comi)utati()ns by means of these factoivs have been found to agree very closely with those obtained in actual experiments, showing that the factors are reasonablv accunite. THE PECUNIARY ECONOMY OF FOOD. The sum expended for food is the princii)al item in the living expenses of a large majority of families, and Net very few of e\en the most intelligent housekeepers have clear ideas regarding the actual nutritive Nalue of difl'erent food materials. Very generally, even those who endeavor to economize know little of the combinations which are best fitted for nourishment and have still less information as to the U. S. Dept. of Agr., Office of Expt. Stations, 71; Plate VI. protein. Fhts Carbohydrates Fuel Value. FOOD MATERIALS Prue pound ec^ 7V« rents will Xv/€> Pounds o{nu,Lritnts aruL talones of^ fuft value in. 10 cenb worth. lib zlbs ilbs 7000 (hi foooCai 6000 Cat/ Jlcey, lOAMvd i\ , SA.ltOA, %ic^ , -a*3ou£cW &(i\AJOCav\/, Zi. .67> <&zXi,X.o I CO taclftefe , /TAVwic' I.YJ &v^iXO, ^$ /ta^x-xymvtt' .bi I a>1l\C& , ^/i(A\Xs ^utvtt' 'i'-Vcittl MO tn: m v^f-'vii:-?! \2c\AS , 2t ccivla' .c>a:;cu ■VSiliatAc.^5 VSHp^jik/^^c-v^-^. 1?,. f7 koo (S, -vefetti . c!tiCi? »3a\ e'j la loes , SCcent? MtM^hii Q>v \&Z Pecuniary Economy of Food. Amounts of Actually Nutritive Ingredients Obtained in Different Food Materials for 10 Cents. Amounts of nutrients in pound.s; fuel value in ealories. 19 relation between the true nutritive value of foods and tlieir cost. Of the different food materials which are palatable, nutritious, and other- wise suited for nourishment, the consumer wishes to know what ones are pecuniarily the most economical; in other words, which foods fur- nish the largest amounts of available nutrients at the lowest cost. In answering" this question it is necessary to take into account not only the prices per pound, quart, or bushel of the different materials, but also the kinds and amounts of the actual nutrients they contain and their titness to meet the demands of the l)ody for nourishment. The cheapest food is that which supplies the most nutriment for the least mone}'. The most economical food is that which is cheapest and at the same time best adapted to the needs of the user. In nran}" of the nutrition investigations, especially- the dietary stud- ies carried on by this Department, the cost of food in relation to the nutrients furnished has been considered. Plate VI, which compares a number of common foods from a pecuniary standpoint, shows the amounts of the protein, fat, carbohydrates, and energy which 10 cents worth of each of the food materials selected will supply. In every case the assmiied price per pound is an average value based upon a considerable amount of data collected in different localities. THE EDUCATIONAL INFLUENCE OF THE NUTRITION INVESTIGA- TIONS. If the piactical usefulness of these investigations is important, the educational influence is no less so. This is manifesting itself in a num- l)er of wa3's, but most of all in bringing the results of the inquirj' directly into schools. Taking all the public schools and the colleges in the country together, the number in which the results of these inquiries are being directly taught is relatively small. Nevertheless, the actual number of institutions in which teachers are including more or less of these results in their courses of instruction, especially in phys- iology, is numericall}" large and is growing with the most encouraging- rapidity. It has been the policy of the Department to deal very gen- erously with schools and with teachers in the distribution of nutrition publications. Not only in cities, but in rural districts, there is a large and rapidl}' growing demand from the schools for these publications. The}' appear to meet an actual want — one that has been rather dimly felt hitherto, but is now becoming much more detinite. The most active call, as would naturally be expected, has been from teachers connected with technical schools or the technical departments of colleges and high schools. The demand, however, has been almost as great from schools of medicine. That the time for the development of these inquiries is especially opportune is shown by the use made of the results in the teaching of what is called domestic economy, or household economics. This rep- resents an educational movement of greater import than many I'ealize. 20 The movement is coming- in response to popular demand and lias the earnest support of many of our leading educators, not a few of whom are emphatic in the expression of their belief in the wisdom of the popular demand and the possibility of making such instruction very useful, especially in courses for girls and young women. Educational experience shows that a certain time is required to bring an}' new subject lirst into scientific and then into pedagogic form. The science of food and nutrition has already assumed reasonal)ly clear and accu- rate scientilic form and is being rapidly brought into pedagogic form. CONCLUSION. One most important feature of these investigations is the coopera- tion with scientilic. educational, and philanthropic institutions in so many parts of the countr}'. Among the advantages of this method of cooperation several are especially worthy of mention. First, there is the larger econom}" and effectiveness of scientific eti'orts shown in the development of special methods of investigation, in the planning of general and special lines of inquiry, and in the comparison and publi- cation of results. The cooperating investigators and institutions are contributors to the enterprise, and the spirit of cooperation thus becomes in itself an important agency for diffusing the results and insuring their most useful application. Another advantage is found in the fact that, while institutions and investigators have that liberty of initiative and action which is so essential for scientilic research, tlie several inquiries are so coordinated, and investigators are so aided by counsel and b}' the collating of the results of inquiry elsewhere as to give both the individual investigations and the research as a whole far more influence and usefulness than would otherwise be possible. From the practical standpoint also there is an advantage in the fact that so many different institutions, representing the varied interests of people in widely separate regions, are united in the study of preva- lent conditions and in efforts toward improvement. Besides this the funds provided b}' the Department are used economical!}' and are supplemented by the resources of the institutions and often l)v means from other sources. Thus not only is a large amount of Avork being- done, but the interest is widel}^ extended and the results are given very great practical usefulness. So it has come about that from modest beginnings, in which work was carried on largely with the aid of private individuals, these inves- tigations under the auspices of this Department have assumed a mag- nitude quite out of proportion to their actual cost; have achieved a noteworthy significance in scientific, educational, sociological, and economic results; and by the extensive coo})eration of individuals and institutions of various kinds with this Department a large amount of valuable work is being done in a systematic way, the results of which are made available to the i)ublic. O LIST OF PUBLICATIONS OF THE OFFICE OF EXPERIMENT STATIONS ON THE FOOD AND NUTRITION OF MAN-Continued. Bui. 89. Experiments oil the Effect of Muscular Work upon the Digestibility of Food and the Metab- olism of Nitrogen. Conducted at the University of Tennessee, 1897-1899. By C. E. Wait. Pp. 77. Price, 5 cents. Bui. 91. Nutrition Investigations at the University of Illinois, North Dakota Agricultural College, and Lake Erie College, Ohio, 189G-1900. By H. S. Grindley ant} J. L. Sammis, E. F. Ladd, anil Isabel Be vier and Elizabeth C. Sprague. Pp. 4i2. Price, 5 cents. Bui. 98. The Eft'ect of Severe and Prolonged ;Museular Work on Food Consumption, Digestion, and Metabolism, by W. O. Atwater and H. C. Sherman, and the Mechanical Work and Efficiency of Bicyclers, by R. C. Carpenter. Pp. G7. Price, 5 cents. Bui. 101. Studies on Bread and Bread Making at the University of Minnesota in 1899 and 1900, By Harry Snyder. Pp. f>5. Price, 5 cents. Bui. 102. Experiments on Losses in Cooking Meat, 1898-1900. By H. S. Grindley, with the coopera- tion of H. M(tCormack and H. C. Porter. Pp. 64. Price, 5 cents. Bui. 107. Nutrition Investigations among Fruitarians and Chinese at the California Agricultural Experiment Station, 1899-1901. Bv M. E. Jaffa. Pp.43. Price, 5 cents. Bui. 109. Experiments on the ^Metabolism of Matter and Energy in the Human Body, 1898-1900. By W. O. Atwater and F. G. Benedict, with the cooperation of A. P. Brvant, A. W. Smith, and J. F. Snell. Pp. 147. Price, 10 cents. Bui. 116. Dietary Studies in New York City in 1896 and 1897. By W. O. Atwater and A. P. Bryant. Pp. 83. Price, 5 cents. Bui. 117. Experiments on the Effect of Muscular Work upon the Digestibility of Food and the Metab- olism of Nitrogen. Conducted at the University of Tennessee, 1899-1900. By C. E. Wait. Pp. 43. Price, 5 cents. Bui. 121. Experiments on the Metabolism of Nitrogen, Sulphvir, and Phosphorus in the Human Organism. By H. C. Sherman. Pp.47. Price, 6 cents. Bui. 126. Studies on the Digestibilitv and Nutritive Value of Bread at the University of Minnesota in 1900-1902. By Harry Snyder. Pp. .^2. Price, 5 cents. Bui. 129. Dietary Studies in Boston and Springfield, Ma.ss., Philadelphia, Pa., and Chicago, 111. By Lydia Southard, Ellen H. Richards, Susannah U.sher, Bertha M. Terrill, and Amelia Shapleigh. Edited by R. D. Milner. Pp. 103. Price, 10 cents. Bui. 132. Further Investigations among Fruitarians at the California Agricultural Experttnent Station. By M. E. Jaffa. Pp. 81. Price, ."> cents. Bui. 136. Experiments on the Metabolism of Matter and Energy in the Human Body, 1900-1902. By W. O. Atwater and F. A. Benedict, with the cooperation of A. P. Bryant, R. D. Milner, and PiTul Merrill. Pp. ,3.57. Price, 20 cents. Bui. 141. Exjieriments on Losses in Cooking Meat, 1900-1903. By H. S. Grindley and Timothy Mojonnier. Pp. 96. Price, 5 cents. Bui. 143. Studies on the Digestibility and Nutritive Value of Bread at the Maine Agricultural Experi- ment Station, 1899-1903. By C. D. Woods and L. H. Merrill. Pp. 77. Price, 6 cents. farmers' bulletins. *Bul. 23. Foods: Nutritive Value and Cost. By W. O. Atwater. Pp. 32. Bui. 34. Meats: Composition and Cooking. By C. D. Woods. Pp. 29. Bui. 74. Milk as Food. Pp. :39. Bui. 85. Fish as Food. Bv C. F. Langworthy. Pp. 30. Bui. 93. Sugar as Food. • By Marv H. Abel. Pp. 27. Bui. 112. Bread and the Principles of Bread Making. By Helen W. Atwater. Pp. 39. Bui. 121. Beans, Peas, and other Legumes as Food. By Mary H. Abel. Pp. 32. Bui. 128. Eggs and their Uses as Food. Bv C. F. Langworthv. Pp. 32. Bui. 142. Principles of Nutrition and Nutritive Value of Food. Bv W. O. Atwater. Pp. 48. Bui. 182. Poultry as Food. By Helen Atwater. Pp. 40. CIRCUL.VR. Cir. 46. The Functions and Uses of Food. By C. F. Langworthy. Pp.10. SEPARATES. *Food and Diet. By W. O. Atwater. Reprinted from Yearbook of Department of Agriculture for 1894. Pp. 44. • Some ResuKsof Dietary Studies in the ITnited States. By A. P. Bryant. Reprinted from Y'earbook of Department of Agriculture for 1S9S. Pp. 14. Development of the Nutrition Investigations of the Department of Agriculture. By A. C. True and R. D. Milner. Reprinted from Yearbook of Department of Agriculture for 1899. Pp. 16. The Value of Potatoes as Food. By C. F. Langworthy. Reprinted from Y'earbook of Department of Agricnlmre for 1900. Pp. 16. Dietaries in Public Institutions. By W. 0. Atwater. Reprinted from Yearbook of Department of Agriculture for 1891. Pp.18. The Cost of Food as Related to its Nutritive Value. By R. D. Milner. Reprinted from Yearbook of Department of Agriculture for 1902. Pp.19. Scope and Results of the Nutrition Investigations of the Office of Experiment Stations. Reprinted from Annual Report of the Office of Experiment Stations for the vear ended June 30, 1901. Pp. .50. Dietary Studies of Groups, Especially in Public Institutions. By C. F. Langworthy. Reprinted from Annual Report of the OJBice of Experiment Stations for the year ended June 30, 1902. Pp. 34. ©(tl LB Mr '05