S H T6 FISH-CULTURAL PRACTICES IN THE UNITED STATES BUREAU OF FISHERIES From BULIvETIN OF THE BUREAU OF FISHERIES, Volume XXVIII, 1908 Proceedings of the Fourth International Fishery Congress : Washington, igo8 WASHINGTON ::;::: GOVERNMENT PRINTING OFFICE :::::: 1910 Class :^B \ 5 \ Book _— L^ FISH-CULTURAL PRACTICES IN THE UNITED STATES BUREAU OF FISHERIES From BULLETIN OF THE BUREAU OF FISHERIES, Volume XXVIII, 1908 Proceedings of the Fourth International Fishery Congress : : Washington, igo8 -\;\ WASHINGTON :::::: GOVERNMENT PRINTING OFFICE 1910 5^ s^^ BUREAU OF nSHERIES DOCUMENT NO. 675 Issued April, 1910 016i OS >^!l' ■ ^ to 3 feet deep, and are placed in the mouth of a small creek tributary to the Potomac reached by tide water from the river. A small number of fish are also kept in a large tank at the hatchery, through which there is a constant flow of river water in connection with the regular station supply. The collecting of fish begins about the middle of March or when the water temperature ranges from 34° to 37°. The water temperature in which spawn- ing occurs ranges from 42° to 46°. In 1908 the first eggs were obtained March B. B. F. 1908—45 7o6 BULI^ETIN OF THE BUREAU OF FISHERIES. 21 in a water temperature of 46°. Practically all the fish had finished spawning on April i, at which time the water temperature was 58°. The spawn is taken daily from the crates and transferred to the hatchery in buckets. The eggs are comparatively heavy, and are slightly adhesive when first deposited. The period of incubation in a water temperature of 47° to 54° is ten to twenty days. The absorption of the yolk sac requires a period of eight days in a mean temperature of 54°. On the Potomac River the average number of eggs from fish of one-half pound weight is about 15,000. When measured into the jars the eggs are computed at 100,000 to the quart. Actual measurements of 10 eggs average 1.662 millimeters or 0.065 inch in diameter, but on account of the peculiar gelatinous envelope of the egg, these individual egg measurements are of no use whatever in estimating the number in a given volume. STRIPED BASS. At Weldon, N. C, is a field station for the propagation of the striped bass {Roccus lineatus). It has been operated for a number of years with rather negative results as to the number of eggs collected, but with experience of much value as suggesting lines of improvement in methods of manipulating ripe fish and the eggs. The chief obstacles to successful work in the propagation of this fish are the difficulties of obtaining ripe spawning females in numbers sufficient to pro- duce large results, and of obtaining a ripe male at the time a female is available. Penning the fish has not proved successful. The delicate nature of the eggs and the limited period of incubation — 1% days — are reasons for believing that they can not be successfully transported from the source of supply. The McDonald automatic hatching jars have been somewhat successfully used at Weldon, but the sac fry of the striped bass are more tender than those of any other fish and it was found that many were injured in their passage to the aquarium through the rubber tube of the closed top. The style of jar was then changed to open top, and has given highly satisfactory results. An elongation of the pitcher mouth by means of a trough of canvas delivers the fry from the jar to the aquarium without friction and concussion. Owing to the buoyancy of the eggs less than one quart of water per minute is supplied to each jar and at time of hatching only about one pint. When water hardened the eggs are computed at 35,000 to the liquid quart and it is customary to incubate 80,000 in each McDonald jar. In the latitude of Weldon, striped bass spawn in a temperature of 70° to 77°, the season being from the middle of April to early in June. Bui,. U. vS. B. F., 190S. Plate LXXIX. Fig. 5. — Spawiitaking operatious oti the Detroit River, Michigan. Whitefish caught in commercial fishing are held in crates and pounds until ripe. The crates are provided with false bottoms, wliich may be raised for easier access to the fish. Fig. 6.— Two men with dip nets are lifting Iisli In^ni crates (male whitcnsli m i.iie crate, fL-niaks in anntherj into the tubs at the spawntaker's right. The pan of eggs is passed to the man at the extreme left, who " washes them up " before they are put into the neighboring tub for water hardening and removal to the hatcher>'. (Detroit River. Michigan.) FISH-CUI^ inches long by c)yi inches wide, on each of which may be placed, if crowded, 10,000 eggs. The total maximum capacity of each pair of Lonffifudino/ Secfion Fig. I.— Clark-Williamson trough. troughs is then 4,800,000. For best results, 8,000 eggs to the tray, or a total of 3,840,000 to each pair of troughs, is a proper number. During incubation the eggs seem to do equally well in either up or down current of the Clark- Williamson troughs ; at other stations where eggs are incu- bated in stacks of trays the Williamson type of trough is used. As lake trout eggs are taken from fish that have been caught in nets hauled into fishing tugs by steam power, often during rough weather and frequently after the nets have been inaccessible for several days, the percentage of good eggs is not equal to that secured from most of the species manipulated. Con- sequently a large force of young women, who are more deft with their fingers than are men, are temporarily employed to pick over the eggs. A shallow trough with water flowing through it is provided for this work; in this trough, standing 1 For full description of these troughs see Manual of Fish Culture, p. 97-99. 712 BULLETIN OF THE BUREAU OF FISHERIES. above the water on four short legs, are wire baskets, one for each egg picker, into which to throw the dead eggs. The farther side of each basket, to the picker's right, has a high back to stop the eggs as thrown from the tweezers, thus making it unnecessary for the eyes of the picker to follow each egg, and thereby facilitating the entire operation. THE BROOK TROUTS. CHARS. AND EASTERN SALMONS. For brook trout eggs {Salvelinus fontinalis) the Bureau depends largely upon commercial trout raisers, eyed eggs being obtained from them at lower cost than it is possible to collect from wild fish at most places or from brood fish maintained only for their eggs. About 8,000,000 eggs are annually pur- chased from ten to eleven dealers. For the purpose of making a just com- parison as to quality and final cost of fish produced from each purchased lot the eggs received from each dealer are distributed to several hatcheries, that all may be alike subject to different conditions of quality and temperature of the water supply. At some stations, however, eggs from wild trout are more satisfactory. It has been found that eggs from the domesticated fish hatched and reared in spring water which is not subject to seasonal variations do not produce good results where the temperature of the water supplying the hatchery is below 35° or is subject to variations of several degrees. Vermont and Colorado are the only states in which eggs of the wild brook trout are collected in sufficient numbers to stock the Bureau's hatcheries in those states as well as to have a surplus for distribution to other hatcheries. It is interesting to note, further, that in Colorado, where the eastern brook trout is an introduced species, the eggs can be collected in greater numbers and at less cost than in any other state. In Vermont" eggs are obtained from trout inhabiting artificial lakes on private preserves. During September and October principally, but in some localities beginning sometimes as early as July and continuing into Novem- ber, the fish ascend the streams in large schools on each rise of water. The fish culturist has only to provide suitable racks and traps in anticipation of the period of migration, constructing them in the streams that have been dammed to make the lakes. The fish are dipped from the trap into adjacent pens above the rack, the pens being kept covered to guard against the escape of the fish m case of a possible flood. A field station of this character is sometimes managed by one man, who constructs the trap, rack, and pens, cares for and strips the fish, and then cares for the eggs, which are incubated until eyed in stacks of trays in the William- son type of troughs, then are packed and shipped to the central station at St. Johnsbury. »Titcomb,*J. W.: Wild trout spawn; methods of collection and utility. Proceedings of the American Fisheries Society for 1897, p. 73-86. BuL. U. S. B. F., 1908. Plate I.XXXI. Fig. 9.— Capturing spawning blackspotted trout in small tributary of Grand Rlcsa Lake, Colorado. Fig. 10. — Trap fur o.tiiiiiniiL,- ■-pa\\-niii- r.tinliow trout at niuiUh <>i priii Colorado. ■ il triliut,i:\- 1.. I. ;kr -^t. Chn-lnbal, FISH-CULTURAL PRACTICES IN THE BUREAU OF FISHERIES. 713 The construction of the racks under the many varying conditions which are wont to prevail requires good judgment and extreme care. Such barriers must be made in anticipation of and providing for floods and must be fish-tight. One small hole large enough for the entrance of one fish may result in the escape of the entire lot. The eying stations adjacent to these collecting stations are small inexpensive structures, a shanty 12 by 16 feet being adequate to eye a million eggs. To compensate for the eggs taken from these waters, about 25 per cent of the fish produced therefrom are returned to them, this being an ample propor- tion to keep them well stocked. The parent fish are always returned to the waters from which they were captured. Eggs of the landlocked salmon {Salmo sebago) are collected by methods quite similar to those pursued in the brook trout work. Although the range of this species has been extended, the field of egg-collecting operations is almost exclusively the native habitat in Maine. Perhaps the most extensive trout-culture operations in the world are con- ducted from the station at Leadville, Colo., in connection with which are field stations for the collection of eggs of wild trout of three species. The output of the lycadville station for 1908 was as follows: Species. Eggs. Fry. Fingerlings. yearlings, and adults. Landlocked salmon 8. 400 144- 000 210, 000 380. 500 45. 000 I. 404. 100 1.833,900 100. 000 3, 736,000 I, 905. 000 50.000 Brook trout Grayling ._ . .. _. ._ _ __ SPAWNTAKING IN COLORADO. To see the methods of work in Colorado it may be well to follow the spawn- takers as they leave their camp on one of the Grand Mesa Lakes for a day's work with the native trout of the Rocky Mountains {Salmo darki) at Big Island Lake, 10,000 feet above the sea level. Each spawntaker is provided with a neck yoke and two lo-quart buckets in which to bring in the results of the day's operations. The fish have assembled in great numbers around the outlet of the lake, where as many as can be con- veniently handled are caught at each haul of the seine and the ripe ones imme- diately stripped. Work at this point may continue all day or it may be advisable after a time to seek other spawning grounds, perhaps at the mouths of small inlets where the water from melting snow is flowing into the lake. A most interesting phenomenon in connection with this work is the run of trout around the island from which the lake derives its name. Every two or 714 BULLETIN OF THE BUREAU OF FISHERIES. three years, and possibly more often, at some period during the spawning season there is a procession of fish in twos, threes, and fours around this island. They follow the indentations of the shore line closely. There is no apparent break in the procession, the line being visible from any view point on the shore. It usually continues, moreover, for several days. A 200-foot Baird collecting seine run from the shore line of the island to form an obtuse angle intercepting the run for ten minutes will be full of fish. The spawntakers, standing about the bag of the seine, in two or three feet of water, proceed to strip the fish in the seine while the procession closes in, the line of trout winding in and out about the legs of the men and apparently in as large numbers as before. The spawntakers with their full pails of spawn proceed to a station near their headquarters, where all eggs are spread on trays and the latter are stacked closely in Williamson troughs supplied with water from an adjacent lake. Here the eggs are eyed preparatory to shipping a portion of them to the Leadville and other stations. Some are hatched at the field station to replenish the waters from which they were collected, or other waters in the vicinity. REARING METHODS. At most stations a portion of the fry are reared to fingerlings, and at some stations it has been found advisable to carry brood fish, both of brook trout and rainbow {Salmo irideus). The latter, a native of the streams on the Pacific coast, has been domesti- cated and successfully propagated at stations in Missouri, Iowa, Virginia, West Virginia, and Tennessee. At stations farther north whose minimum water tem- perature is usually lower and subject to extreme changes, it has been cultivated with varying, but on the whole rather negative, results. It has been successfully acclimatized in some of the more northerly states, notably in Michigan; but it does not thrive in waters subject to extremely low temperatures during the winter months, and in New York and the New England States has proved in most streams a failure. The domesticated brood fish of either species are usually the product of eggs collected from wild fish, and are reared in the usual manner. The young fish may be confined for the first four or five months, or until 3 to 5 inches in length, in the hatching troughs or in a battery of outdoor rearing troughs of dimensions and in other respects quite similar to the indoor troughs, about 12 feet long by 14 inches wide. Care must be taken, however, to guard against overcrowding as the alevins increase in growth. The actual number of young fish of a given age which can be successfully carried is dependent upon the quality of the water supply, temperature being an important factor, not only as to the number for a given space, but also as to their rapidity in growth. At the White Sulphur Springs (W. Va.) station, with a supply per trough of 10 BuL. U. S. B. F., 1908. Plate LXXXII. -l-iuld hatchciy at Graiul :Mcsa l,akub, Colorado. Blackspotted trout eggs Uj thu iiiimbL-r uf 7,wo,ooo in one season have been developed here to the eyed stage with only a normal loss. Fig. 12. — Tray of trout eggs in hatching trough. The fry fall or work through the rectangular niesh of the tray bottom into the trough, where they are visible at the left of this picture. Tray is wedged at proper depth in trough during incubation, and, for convenience in removing dead eggs from time to time, may be floated by releasing the wedges. FISH-CULTURAL PRACTICES IN THE BUREAU OF FISHERIES. 715 gallons of water per minute at a temperature of about 50°, it is customary to hatch and hold in each trough 50,000 sac fry, 25,000 advanced fry, 12,500 i}^- inch fingerlings, 4,000 i^^-inch fingerlings, 2,000 i^-inch fingerlings, and 1,000 fish 2 to 3 inches in length. Much larger numbers are often carried under simi- lar conditions without serious loss, though often with the result that the fish prove weak in transportation. At stations where the facilities permit, a congested condition of the hatch- ing troughs is avoided by transferring some of the fish to outdoor ponds as soon as they have learned to take food readily, or, if weather conditions are suitable, after being fed two or three weeks. In rearing fingerlings for four to six months, concrete ponds 18 to 25 feet in length by 5 to 6 feet in width and 2^4 feet deep, with a fall of 8 to 10 inches in the bottom for drainage, give good results at the Manchester (Iowa) station. The stock ponds at Manchester, 76 feet long, 17 feet wide, and 3 feet deep, supplied with 40 gallons of water per minute at a temperature of 50° to 60°, have a capacity for 3,500 rainbow trout 2 years of age; 1,800, 3 years old; 1,000, 4 years old, and 900, 5 years old. This trough and pond system is typical for rearing any species of brook trout, as well as lake trout and Atlantic and landlocked salmons, for three or four months, which is as long as it is customary to hold young fish intended for dis- tribution. Brood fish may be obtained by selection from the fingerlings intended for distribution, which as they develop are transferred to stock ponds. As soon as the fry swim up looking for food they are fed several times a day an emulsion of finely ground liver. This diet is continued as the young fish develop, with the difference that the liver is less finely ground and is given less frequently — two or three times a day being sufficient when the fish have attained a length of 2 or 3 inches. The kind of liver used varies at different stations, that of sheep, beeves, and hogs being extensively used, and the relative value of each being in the order named. The food for the larger fish consists of the liver, lungs, and hearts of the animals already mentioned. At Manchester, Iowa, it has been found advantageous from an economical standpoint to mix the animal food, after it has been ground, with a mush made by cooking wheat middlings or shorts, to which a moderate amount of salt is usually added. After the mush has been thoroughly cooled the animal matter, uncooked, is stirred into it in the following proportions: For fingerlings, i part animal matter and 2 parts mush; for adults, i part animal matter and 3 parts mush. Twenty gallons of boiling water and 50 pounds of wheat middlings will make about 202 pounds of mush. The provision of food for domesticated fish is one of the greatest problems the fish culturist encounters, and has been the subject of considerable experi- mentation. The intensive production of natural food has not received in the United States so much attention as in Europe nor so much as the subject 7l6 BULLETIN OF THE BUREAU OF FISHERIES. deserves. The American method of raising trout precludes the economic use of natural live food, although unquestionably the edible qualities of the fish might be much improved thereby. For several years the roe of the branch herring {Pomolobus pseudoharengus) has been used at several stations as a substitute for liver in feeding fry and fingerlings. It is purchased from cannery men, who preserve in tins large quan- tities of it for human consumption, and consequently are able to sell it at a price close to or less than the cost of liver, due allowance being made for the waste in liver and the labor involved in its preparation as a fish food. The herring roe has the advantage of always being on hand in condition ready to feed and is therefore especially desirable for isolated stations. It is not customary to feed young fishes on this food after they are 2 or 3 months old. At Craig Brook, Me., fly larvae," used for a number of years in rearing trout and salmon to fingerlings, proved to be a very satisfactory food and the fish attained a more rapid growth than when fed on liver and other dead material. From a financial standpoint it was not as economical as freshly prepared liver or other animal foods, and this, coupled with the objectionable odor attendant on its preparation, had much to do with its discontinuance. It is possible, however, to utilize animal refuse advantageously for the pro- duction of fly larvae by means of a contrivance in which the material on which flies have deposited their eggs may be suspended over the water. This con- trivance consists of a wooden frame, like a box without top or bottom, placed on floats and having an air-tight cover to prevent the escape of foul odors. Within the frame are two trays, the bottoms of which are made of coarse wire cloth (odds and ends of old hatching trays). Excelsior or straw is placed on the trays, and waste meat or other animal refuse is placed on top of this material. As the larvae hatch they work through the excelsior, cleaning them- selves thereby, and drop into the water. Two small trays are preferable to one of larger size, that the meat may alternately be renewed, thus insuring a more constant supply of larvse. The noxious animals killed in the protection of fish may advantageously be disposed of in these trays. SPECIAL DEVICES APPLIED AT TROUT STATIONS. Study and experience in recent years have revealed abnormal aeration as a condition existing in various water supplies at trout-culture stations, with consequent mortality among the fish. Of the methods used to correct such abnormalities, that devised by the superintendent of the station at White Sulphur Springs, W. Va., Mr. R. K. Robinson, has proved the most efficient. » Atkins, Chas. G. : The live food problem, American Fisheries Society, 1903; also Food for young salmonoids, Proceedings Fourth International Fishery Congress, Bulletin Bureau of Fisheries, vol. xxviii, 1908, p. 839-851. FISH-CULTURAL PRACTICES IN THE BUREAU OF FISHERIES. 717 It consists of a series of ordinary milk pans held in a frame, one above another, in numbers to suit conditions, the bottoms of the pans perforated with a nail or other pointed instrument which will leave a ragged edge to each perforation on the underside of the pan. This apparatus is set up at the head of the trough in such position that the supply pipe empties into the topmost pan, and the water must pass through the series before reaching the trough. By this separation into fine streams the water is thoroughly exposed to the air, thus rectifying any abnormality of air content. At nearly all trout hatcheries it has been customary to place horizontally below the supply pipe at the head of each trough a screen consisting of a light frame, bottomed with wire cloth or perforated metal. This is designed not only to break the force of the stream entering the trough but to aerate or deaerate the water and at the same time catch foreign substances and animal life — the latter at times being quite objectionable. Such screens, however, have almost invariably caused the water to spatter over the sides of the trough, resulting in constantly wet surroundings. To overcome this objectionable feature a conical perforated screen has been devised by Mr. M. E- Merrill, of the St. Johnsbury, Vt., station. When the screen is in place the current of water falls directly on the apex of the cone, and thus is spread over the entire perforated surface, accomplishing the objects of all other styles of head screens, and avoiding the spattering of water over the sides. A device for assorting young salmon and trout was introduced in the Bureau's operations by Mr. J. P. Snyder, an employee at one of the stations. It consists of a series of screens by means of which to separate the fingerlings into sizes. In length the screens are slightly less than the width of the troughs, to facilitate sliding them along. There should be two screens for each size of mesh. In use one screen is carefully inserted at the foot of the trough close to the tail screen, due precaution being taken that no fish are pinched and that none are left between the foot screens and the end of the trough; midway of the trough a screen should be securely fastened in a vertical position by wedging. After the first screen is in position a similar one is inserted at the head of the trough and then moved along toward the center. As the two screens are brought closer together the fish between them become frightened, and all that are small enough escape through the mesh of the screens. The distance of the second screen from the first should depend upon the number and size of the fish in the troughs, and also upon the number that escape through the screen. The second screen should also be fastened by wedging. Then the hand of the attendant is moved about among the fish between the screens to guard against any small ones finding a hiding place. In 71 8 BULLETIN OF THE BUREAU OF FISHERIES. their efforts to escape some of the fish will be hung in the wire cloth, but it will be noticed that every trout which gets its head through the screen can pass or be assisted through without injury. The few which are caught in the mesh should be assisted by grasping the tail and pushing them. It would be well to refrain from feeding for about twenty-four hours before assorting the fish. By using two or three sets of screens in different troughs at the same time one man can assort many thousands of fish in a day, and the sizes will be much more uniform than when assorted with a scaff net. Wire cloth, 6 bars to the inch each way, painted with asphaltum varnish, will permit all brook trout under i inch in length to pass through. By varying the mesh of screens brook trout may be assorted into six uniform sizes as follows: Number of bars to the inch : Size of fish. 6 All under i inch. 5 All between i inch and i J^ inches. "■ 4 All between I J-2 and 2 inches. 3 All between 2 and 2 H inches. 2/5 All between 2}'s and 3 inches. 2 All between 3 and 31$ inches. The frames of these screens are made of half-inch wooden strips grooved and tongued at the ends. These frames are one-eighth inch less in length than the inside width of the troughs and in height equal the depth of the troughs, being rectangular in form. They are covered on one side with wire cloth held firmly by copper tacks, both the wire cloth and the frames being painted with asphaltum varnish previous to tacking the wire on the frames. This not only helps to preserve the wood and keep the wire from rusting, but smooths the latter so that there are no rough surfaces or projections to injure the fish as they work their way through. ATLANTIC SALMON. Another important branch of fish culture is conducted at the Craig Brook station, near the Penobscot River, not far from Bucksport, Me. While not restricted in its work to this one species of fish, the primary object of this hatch- ery is the propagation of the Atlantic salmon. The decadence of this important fishery on the North Atlantic coast, due to the ruthless but natural progress of civilization, is too well understood to call for an explanation here. Suffice it to say that to-day the Bureau is maintaining a commercial fishery for the Atlantic salmon on the Penobscot River purely by artificial propagation. It is the only river in the United States where this once abundant salmon is now found in sufficient numbers to support a fishery or to warrant its artificial culture, and here, with the natural conditions so changed, it is with no little difficulty that the extinction of the species is prevented. BuL. U. S. B. F., 1908. Plate LXXXIII. 1 1 1 pPi Si K« P"^^ / ^^J^ a^Z^^I^S ■ ,_ \_ ..:^^ ""^ll^ ^^^^^ ^^^^ r ^^^1 K;^ ^Bt^3E^^^^^^C^^^^^^^^^gr~"_ niiT." 3t ^■■■fei ^^^^^^ ^HHi^H^k^ Fig. 13— Series of covered trout-hatching troughs at White Sulphur Springs station (West Virginia) equippt.(i with thr R..biiisnn pan aerator. (See p. 716.) Fig. 14. — Trout-hatching tninyhs wiih Merrill aerating coni-, (Suf p. 717.) shows also tray basket <'t a I'.ittern used at some trout stations, much deeper than ordinary tray and intended to hold fry after hatching. l^eft trough with tray removed to show shower of water produced by aerating cone. FISH-CULTURAL PRACTICES IN THE BUREAU OF FISHERIES. 7 19 The operations at this hatchery, fully described in the Manual of Fish Culture published Ijy the Bureau of Fisheries in 1900, have undergone slight change of method and need not be dwelt upon here. The source of egg supply is the catch of the fishermen's weirs usually during the month of June, the fish being purchased and towed in live cars to the station, where they are trans- ferred to inclosures and there retained until the spawning season, in October and November. When ripe they are stripped and the eggs placed upon wire trays, which are stacked in troughs and carefully tended until the early spring, when the eggs hatch. The young fish are distributed for the most part as fry, but a considerable number are reared to the fingerling stage. POND CULTURE. Pond culture in the United States is applied only to nest-building fishes, such as the basses, sunfishes, and the common catfish {Ameiurus nebulosus). These species do not submit to manipulation for taking and fertilizing their eggs, but fortunately a very large percentage of the eggs are fertilized when the spawning functions are permitted to occur naturally, and the parent fish care for and protect the young until the latter are free swimmers. The cultivation of these fishes, therefore, consists in providing ponds which shall give to the maximum number of breeding fish and their young all the essential conditions of a natural environment, while at the same time protecting them from their enemies and holding them under control. THE PONDS. Economy in construction usually dictates the shape and area of the ponds, but an independent water supply and drainage to each is desirable. For con- venience of the fish culturist the area usually ranges from one-fourth to one acre, although some ponds of larger size are desirable. It was formerly considered essential to have at least one-fourth the area of the breeding pond not exceeding I foot in depth, but it has been found that the deepening of the shallower por- tions to a minimum depth of from i foot to i yi feet has largely increased the productive area. The presence of aquatic plants in fish ponds is a prime essential. The young of the nest-building fishes do not accept artificial food, and must there- fore have their natural diet of minute animal life, the abundance of which is dependent to a large extent upon the character and abundance of plant growth. Plants are also important as oxygenators of the water and afford shelter and shade for the fish. The selection and control of aquatic vegetation, therefore, is a matter to which the fish culturist must give much attention, and experience at the various stations indicates that it oft'ers a direct means by which the output 720 BULLETIN OF THE BUREAU OF FISHERIES. of the ponds may be increased. The subject has not been sufficiently studied, but observations so far made suggest various practical possibilities of much interest." The supposed loss of young fish by the voracity of their parents induced the practice of partitioning the ponds in such a manner as to confine the adults in one portion while permitting the young to escape through the partitions to safety. It has been found, however, that the loss from cannibalism is due chiefly to the young fish themselves, and accordingly they are separated from their parents or not, merely as a matter of convenience. The principal pre- caution against cannibalism is, instead, the provision of an abundant food supply, to divert the fish from each other. FOOD FOR THE ADULT FISHES. Food for the adult fishes is largely a matter of local conditions and con- venience. Chopped fish is extensively used at some stations, and crawfish, so abundant in some localities, when chopped make admirable food for the adult stock. The basses, although not appearing to care for pollywogs as naturally present in the ponds, will devour frog tadpoles voraciously if the latter are seined out and thrown back by the fish culturist, but they absolutely refuse toad pollywogs when similarly served to them. Minnows are a good food, but should not be introduced into the ponds near the spawning season, as they eat not only the small forms of life upon which the fry depend but often eat the fry as well. Dead minnows thrown into the water one at a time are greedily taken by the adult basses. Adult bass may also be advantageously fed on strips of beef liver about 2 to 3 inches long and from one-half to one-fourth inch in width or thickness; and prepared food consisting of ground liver or other animal substance mixed with a mush of cooked shorts, com meal, or middlings has been employed in a rather limited way. It is worthy of note that for this prepared food to be attractive to bass it must ordinarily contain at least two-thirds of the animal substance, whereas prepared food containing only 10 per cent of the animal material is taken with avidity by trout. It has been quite conclusively demonstrated that one of the principal causes of loss among brood fish is overfeeding, resulting in a fatty degeneration. This loss has been largely overcome by reducing the food supply and at the same time varying the kind of food furnished. ARTIFICIAL NESTS. In the cultivation of the small-mouth bass, and to some extent the other species, it has been found profitable to provide artificial nests. These are of " Titcomb, J. W. : Aquatic plants in pond culture. Bureau of Fisheries Document 643. 1909. BuL. U. vS. B. F., 1 90S. Plate LXXXIV, ^^.. ''I > " ^m^ Fig. 15. — Placing cheese-cloth retainers over nests containing bass fn.- about ready to -^wiiu up. Most of the nests are seen covered with retainers. When a nest is occupied it is numbered and complete record kept of its different stages. The retainers are to confine the fry for convenience in transferring to other ponds to make room for following broods. (Mammoth Spring station. Arkansas.) JO. — Pond drawn down und ne>t buxes phiLt. i 1-: 150 breeding smallmouth black ba: .', i;:il_;. .Vli-ra of pond, o,,.. . .-,....:;. i.i (^Mammoth Spring station, Arkansas. j i.:5L? Ill pond; r I FISH-CULTURAL PRACTICES IN THE BUREAU OF FISHERIES. 72 1 various design, but embody in general some sort of a container for the gravel the fish require, together with a shield or screen on two or three sides." The primary use of screens was for the purpose of shielding the fish from view of passers-by, but the practice resulted in the discovery that the fish will accept shielded nests at more frequent intervals than when visible to each other. It is therefore of first importance that in the placing of nests the screens be arranged to meet this condition. Wlien shielded artificial nests are not provided it is customary to deposit here and there in the ponds mounds of coarse gravel, 18 inches to 2 feet in diameter and about 6 inches in height, that the breeders may select and prepare their nests with these. Large- mouth black bass, though they sometimes accept gravel mounds as nests, naturally seek a weedy bottom, devoid of gravel. Peat-like sods or similar substances put into the pond prove acceptable to this species as nesting material. The superintendent of the Cold Springs, Ga., station endeavors to imitate nature by providing "homes" for all adult fishes whether spawning or not. For the large-mouth black bass and rock bass, boards 3 feet to 10 feet in length are laid flat under the water so that by the natural contour of the bottom spaces 5 inches to 8 inches deep are formed under the boards. Catfish {Amciurus nebu- losus), which prefer to dig their own nests, are provided with boards either laid flat on the pond bottom or where there will be under the center of the board a de- pression an inch or two in depth. Where necessary, the boards are fastened by stakes at both ends, but when placed along the bank, where conditions are favor- able for such a course, one end of the board may be driven a short distance into the embankment, while the other end is staked. After having been submerged a month or so the boards will remain in place without fastening. With proper precautions against projections these shelters do not materially interfere with seining operations. For the crappie, roily water seems to be essential during the spawning season. At stations where there are no naturally roily ponds it has sometimes been found desirable to introduce a few carp, which roil the water in rooting around the bottom of the pond and do not seem to disturb the crappie. NUMBER OF BROOD FISH. The desirability of maintaining a maximum number of brood fish to a given pond area has led to a comparison of experience at the different stations in an effort to arrive at some approximate average for a working basis. Conclusive "One form of nest in use is the design of A. E. Fuller, described in his paper entitled "New and improved devices for fish culturists," Proceedings Fourth International Fishery Congress, Bulletin U. S. Bureau of Fisheries, vol. xxviii, igo8, p. 991-1000, pi. civ-cvi. B. B. F. 1908—46 722 BULLETIN OF THE BUREAU OF FISHERIES. determination can not be made, of course, owing to the various factors of quality and temperature of water, abundance of vegetation and of natural food, etc., but reports from the several localities are of interest. At San Marcos, Tex., the best results are obtained with 24 to 30 large- mouth bass to the half acre; of the smaller fishes — bream, rock bass, crappie — 60 to the half acre. A i-acre pond at Mammoth Spring, Ark., supports 100 small-mouth black bass, and an average of 2,000 fry to each productive nest has been obtained, the maximum number from one nest being 5,200. At Cold Springs, Ga., 100 adult large-mouth black bass in a pond of three- fifths of an acre in area proved too many, and the number had to be reduced to 60 or 70 for satisfactory results. In general, 50 to 75 brood bass to three- fourths of an acre to an acre have been found the best number at this station. Of catfish {Ameiurus nebulosus niannoratus) , 100 to the acre have l)een found satisfactory. Ponds at Wytheville, Va., accommodate 75 pairs of large-mouth black bass to the acre with good results; of rock bass, 300 fish to the acre. At Northville, Mich., in a pond three-fifths of an acre in area it has been observed that most satisfactory results were obtained with small-mouth bass to the number of 29 females and 23 males, allowance being made for the occa- sional polygamous tendency of the male. At White Sulphur Springs, W. Va., 36 pairs of small-mouth black bass is considered the number for a i-acre pond. The maintenance of an abnormally large number of brood fish to a given area results in more or less loss according to species, the mortality among the brood fish of the small-mouth black bass being greater than with the large-mouth bass and other pond fishes. The replenishing of the stock is most advanta- geously accomplished by securing wild fish, preferably in the spring of the year, and they may be advantageously transferred up to within two or three weeks of the spawning season. COLLECTING THE YOUNG FISH. It is often desirable to remove the surplus fry from a pond before they have left their nests, and there is now in use for this purpose a combination fry trap and retainer which is placed over the nests, taking advantage of the fact that the fry rise vertically." This trap has proved of practical value in fish-cultural ponds for small-mouth bass, and, where the nest area was not too great, for large-mouth also. It has been used likewise in collecting small-mouth bass fry from natural lakes, and is believed to be applicable to fry of other nest- building fishes than the basses. "See Fuller, op. cit. FISH-CULTURAL PRACTICES IN THE BUREAU OF FISHERIES. 723 Soon after the yolk sac has been absorbed, or after the fry have been feeding for two or three weeks, a portion of them are removed from the ponds and dis- tributed to the waters they are to stock. The first crop may often be obtained by seining around the edges of the pond without the prehminary clearing away of vegetation, and for this purpose a novel casting seine has come into use at Northville, Mich. The web is rigged upon two long bamboo poles, so that the device may be operated entirely from shore, without roiling the water or unduly disturbing the fish." After the young fish have sought the deeper portions of the ponds, pre- liminary to drawing off' the water to effect their capture, it is necessary to remove the aquatic vegetation, a process of much labor and expense, consisting in general of mowing under water, and carrying away the foliage by means of pitchforks and boats. Various methods and devices for this purpose have been evolved at the different stations, as described elsewhere.'' Ordinarily the assorting of young pond fishes by size is accomplished by hand manipulation with a scaff net. To some extent, however, the separation may be accomplished by regulating the size of the mesh in the nets used to effect their capture. The superintendent of the San Marcos, Tex., station suggests having an ample bag to the dip net in which quite a large lot of fish may be taken from the tub or other retainer, then passing the net gently to and fro in the water to allow the fry and smaller fish to escape, while the larger ones are retained. This method is principally used for assorting black bass, as it frequently happens there are schools of both fry and fingerlings in the ponds at the same time. Nets of one-fourth inch square mesh will permit the escape of all fish up to I j/2 inches ; one-half inch square mesh will permit the escape of all fish tinder 3 inches in length. At the Cold Springs, Ga., station the superintendent uses a box 3 or 4 feet long by I foot wide and i foot deep, and water-tight to a depth of 2 inches. Above this 2 inches one side is covered with wire cloth instead of being closed in, the size of wire mesh being regulated for known sizes of fish. The box is par- tially submerged in the pond in which it is intended to place the smaller fish of a lot to be assorted. The young fish as caught are placed in the box, and then are left undisturbed for an hour or two. At the end of this time the smaller fish will have escaped from the box through the screen into the pond, when the box with the larger fish remaining in it may be transferred to another pond and emptied, or the contents may be poured into a suitable receptacle for transpor- tation by tipping the box toward its solid side. Square-meshed galvanized cloth is used for the screen, and if the fish are given plenty of time to separate none of them are gilled, hung, or otherwise injured. o Fuller, op. cit. 6 Titcomb, J. W. : Aquatic plants in pond culture, Bureau of Fisheries Document 643. 1909. 724 BULLETIN OF THE BUREAU OF FISHERIES. RESCUE OF FISHES FROM OVERFLOWED LANDS. In the upper Mississippi and Illinois rivers there is an annual spring flood period caused by the melting of the snow in the northern forests and freshets in the local tributaries after heavy rains. The period begins with the approach of warm weather, usually about March 15, and continues until about June i, when the crest of the high water has been reached. Soon after this date the water begins slowly to recede, and usually by July 1 5 the river has reached its normal stage. Between the extreme low and high water marks there is a variation of 1 2 or 1 5 feet. There is, of course, a variation in the extremes of the water level in different seasons, but seldom, if ever, does'the water fail to rise high enough to flood the lowlands. The adult fishes are thus permitted to enter the overflow basins and bayous, and invariably do so during the spawning season. After spawning most of the adult fish escape to the river before the water has receded sufficiently to cause them to be hemmed in, but immense numbers of theif progeny are left in the lakes and bayous where they were hatched. These waters gradually dry up, become choked with vegetation, or overheated and unfit for fish life; some of the larger and deeper lakes and bayous, although cut off from the main river, may contain water the year around, but on account of the seepage and evaporation during the summer the depth of water in them de- creases to such an extent that they freeze solid during the winter months. Sometimes the lakes from which fishes are rescued are in the hollows on farm lands, where in dry seasons crops are cultivated. Thus it will be seen that the fish imprisoned in overflow waters are doomed to destruction in one way or another. One branch of the Bureau's operations is annually to rescue large numbers of these fishes. At present the work is confined to waters convenient of access — namely, the overflow lakes and bayous on the low islands in the rivers and on the adjacent mainland. Many of the fishes are returned to the rivers. Another portion of the more desirable species is distributed in various other waters, often far from the source of supply. It has been found, however, that the fishes rescued from these warm waters do not bear transportation long distances without heavy losses if immediately started upon their journey. Therefore a hardening process is resorted to, which consists in holding the fish in large tanks flowing through which are streams of clear cool water. To facilitate the work the Bureau has a number of field stations — one on the Illinois River and three on the Mississippi — convenient of access to the railroad, and each equipped for holding one or more carloads of fishes for several days, or until they have become sufficiently hardened to bear transportation bv cars. Adjunct to these stations are vessels, launches, and boats of various types suited to the work. BuL. U. S. B. F., 1908. Plate LXXXV. Fig. 17. — Both trout and bass are cultivated at many of the stations. This view of the station at Manchester, Iowa, shows stock ponds in foreground, then the smaller nursery ponds, all of these for trout and built of cement. Beyond, in front of the hatchery building, is a bass pond, with earth bottom and sides. KiG. iS. — Preparing a shipment of sniallmouth bass. Tanks used for hardening the young fish prior to transportation. The fingerlings are held for 12 hours in cold s])ring water, then dipped into tubs, counted into pails, and transferred to the transportation cans. These are then placed under a -^-inch stream of spring water and held until train time. For an early morning shipment the fish are "canned" the evening before. (Mammoth Spring station, Arkansas.) FISH-CULTURAL PRACTICES IN THE BUREAU OF FISHERIES. 725 By this means not only is a conservation effected but the Bureau is enabled to meet the great demand of applicants for the basses, sunfishes, and perches at a less sum than it would cost to produce them at a station maintained especially for their propagation. THE PACIFIC SALMONS. On the Pacific coast the Bureau has six permanent stations, including two in Alaska, all of them maintained primarily for the propagation of the Pacific salmons. Subsidiary to these there are six important field stations and other smaller ones where salmon eggs are collected and hatched. An idea of the extent of the work may be obtained from a statement of the output of these stations during the fiscal year 1908. Output of the Pacific Salmons in igo8. Stations. Species. Eggs. Fry and fingerlings. Alaska 61.369. 000 4.780.855 Chinook - 64. 990. 550 3.S30.00O Silver _ 215. 932 498. 309 13. 262. 714 8 5M.30S 6, 764. 762 Chinook Silver 296. 000 75 000 502. 000 The eggs shown in this table were transferred to state fish hatcheries and other places for incubation. In California, as will be noted, a very large propor- tion of the eggs taken are so distributed. Observations at various field stations indicate that a large percentage of salmon eggs deposited naturally are fertilized but for various reasons only a small percentage hatch. Modern fish-culture methods permit of a much higher percentage of impregnation than under natural conditions, it being possible to actually hatch and distribute as fry more than 95 per cent of all eggs collected. So long, therefore, as a proper number of salmon are permitted to escape the various fishing devices in their ascent to the natural spawning grounds, and it is possible to capture them for the purpose of obtaining and impregnating their eggs, perpetuation of the salmon fishery is assured. In the culture of the Pacific salmon it is impossible to save eggs from the commercial catch, because the latter is made before the fish are ripe, and to retain them until ripe is not feasible. By the time they have ascended the rivers to the spawning grounds and are in condition for the fish culturist the flesh has so deteriorated in quality that they are unfit for market in any form. The Bureau must therefore itself capture the fish it requires, and this is usually done by the construction of barricades to intercept the run at the most suitable point below the spawning grounds. 726 BULLETIN OF THE BUREAU OF FISHERIES. BARRICADES AND TRAPS TO INTERCEPT SPAWNING RUNS. That successful work at the salmon stations depends largely upon stable and suitable barricades, or racks, as they are more commonly called, may be instanced by the results of the work at Battle Creek, Cal., in 1903 and 1904. At the height of the season in 1903 a freshet carried away several sections of the rack grating, permitting the fish to escape upstream." As a consequence only about 27,000,000 eggs were secured. The following season no flood occurred at Battle Creek until near the end of the spawning season, and the collections that year numbered over 57,000,000 eggs. As all of the streams are subject to freshets, the water in some instances rising over 20 feet, the racks must be firmly built, and their successful operation depends not only upon ingenuity in construction but the care that is taken to guard against their becoming clogged with leaves and other debris in times of flood — work which at times is exceedingly hazardous. Methods in the construc- tion of racks vary with local conditions, as do also the methods of capturing the salmon thus intercepted. At the Mill Creek station, in lieu of a main or upper rack, the Bureau is able to take advantage of a mill dam 12 feet high, which effectively stops the passage of salmon. Half a mile below this dam a retaining rack with the usual traps prevents the fish from dropping down stream. Seining and spawning operations are conducted on the streams between the dam and the retaining rack. At Baird, Cal. — At the Baird station on the McCloud River in California are two racks or barriers between which is formed a pool 400 feet in length. The upper rack intercepts the further passage of salmon, and the lower or retaining rack gives the fish free entrance to the pool, but effectually prevents their return. The upper rack reaches across the river, a distance of 250 feet, and is primarily supported by 10 concrete piers averaging 8 feet in height and extending 5 feet above low-water mark. The piers are prdperly fastened to the bed rock of the river bottom by means of heavy iron bolts. They have a flat top 4 feet wide and 6 feet long, and from top to bottom is a beveled nose extending upstream at an angle of 60 degrees, making them 4 feet wide and 10 feet long at the bottom. On either bank a small crib pier filled with rock supports the shore ends of two 10 by 10 inch stringers laid parallel from shore to shore across the tops of the piers. A 2 -foot walk is built between the stringers and the whole is securely wired to eyebolts built in the pier tops. Across the river bottom, against the nose of the piers, is a lo-inch sill. At intervals of 3 feet poles 4 inches in diameter extend at an angle of 60° from the sill at the bottom to the stringer at the top, and are securely fastened to the 2 At Battle Creek the low-water mark is lo feet below the top of the stringers on the rack, and during a recent flood the water was 12 feet above the top, making a 22-foot rise. FISH-CULTURAL PRACTICES IN THE BUREAU OF FISHERIES. 727 latter by large spikes. Against these poles or inclined uprights rest the gratings of the rack, which for the sake of convenience in handling are built in sections 6 feet wide and from 6 to 10 feet long to suit the varying depth of water. The gratings are made of i X '^Y 3H inch slats of dressed lumber set i }4 inches apart, their thin edge facing the current, the edge being convex to facilitate cleaning, and permit the passage of leaves. The ends of the gratings are nailed between two pieces of i^4 by 4 inch material, notched into the slats to make a flush surface. The space between the slats is gaged by nailing on i X by 4 inch blocks to each end. The longer gratings are braced with two strips i>^ by 4 inches nailed on 3 feet from the bottom. In the upper rack is placed a trap 10 feet square with vertical slat sides similar to the rack gratings and having a solid board bottom. The narrow opening which allows the fish to enter is so constructed as to reduce to a mini- mum their chance of escape. The trap is primarily used for observing the general condition of the fish in the pool prior to the beginning of seining or spawning operations. The retaining rack is at the lower end of the pool, where the stream narrows to about 190 feet. It is supported on 6 stone-ballasted crib piers with sides 14 feet long, made by spiking together logs 8 to 12 inches in diameter until the required height is reached. The piers are built on shore, floated into place, and filled with rock. Across the upstream end of each pier are two 10 by 10 inch stringers laid parallel and supporting a board walk, as in the upper rack. Two small temporary piers are also built, to support the shore ends of the rack. Gratings having 2-inch interstices are placed across the stream, similar to those in the upper rack, with the exception that 5 openings 2 feet wide are left between the piers nearest the center of the stream. These openings are covered by the usual traps, which extend upstream into the pool g^i feet. The traps are 4 feet in height and 6 feet in width at the entrance, being shaped to fit the slant of the gratings. The sides are of i 'X by 4 inch material spaced 2 inches apart, and with the broad edge toward the current. Braces are placed across the top, and at the apex of the trap is an opening 3 inches in width from the surface of the water to the bottom. The salmon pass into the pool through this opening and rarely, if ever, find their way out. Before the installation of the retaining rack, some ten years ago, many eggs could not be collected by reason of the loss of fish from their running back down- stream. This violation of the natural instinct of salmon to work ever upstream was due to fright resulting from the continual sweeping of the seine just below the upper rack. In the early days Indians were engaged to walk on either shore for a mile or so below the rack and beat the water with brush in an endeavor to drive the fish up to the seining ground. Since the installation of the retaining rack such measures have been entirely unnecessary. 728 BULLETIN OF THE BUREAU OF FISHEiaES. At Battle Creek, Cal. — The main or 'upper rack at Battle Creek, Cal., is constructed on a comparatively soft and shifty river bottom, and is supported by piling, instead of by the log cribs anchored with rocks, more generally used. There are 12 bents of piling, each bent consisting of 3 piles driven firmly and braced with heavy timbers. The 3 piles comprising a bent are driven parallel with the current, the front one standing some 2 feet above low-water mark and the others about 8 feet above. The front and rear piles are placed about 10 feet apart. On these bents of piling and reaching across the stream are placed three 12 by 12 inch stringers, against which are secured 4 by 4 inch slanting supports, about 6 feet apart, the lower ends of which rest on a mud sill placed in the bottom of the stream. Stringers and these supports are so placed that the face of the rack will meet the current of the stream at an angle of about 60°. The gratings of the rack are built in sections of varying length but of a uniform width of 5^ feet. The slats for these gratings are of dressed lumber i by 3 inches, the sides set parallel with the current, the upstream edge convex. At either side of the stream, in the shallower water, single sections of gratings about ID feet long extend from the bottom to the top stringer. In the deeper portions of the stream two 6 or 8 foot sections of the gratings are used, one above the other, with an opening between the upper and lower sections for convenience during the lower stages of water in the removal, with rakes and hooks, of rubbish and trash drifting downstream. When the water rises the closing of this aperture is easily accomplished by knocking out the blocking between the two, thus permitting the upper rack to slide down flush with the upper edge of the lower section. The length of the rack from shore to shore is about 300 feet, its vertical height above low-water mark being about 8 feet. During low water the front is submerged to a depth of from 18 inches to 2 feet, but there are holes considerably deeper behind the rack. A walk 2 feet wide is built on the top of the rack. A half mile below the barrier is a retaining rack quite similar to the one described for the Baird station. At Battle Creek the racks are usually installed during September in time to intercept the fall run of salmon, and unless carried out by high water, remain until the close of the work in December. Gratings, stringers, etc., are then removed and stored for use another season. At Birdsview, Wash. — A permanent barrier at the Birdsview station, an auxiliary of the Baker Lake station, in Washington, is of novel construction and calls for more than passing notice. This barrier is located in a portion of Phin- ney Creek where formerly there was a dam built for the purpose of obstructing the passage of steel-head trout. When the dam washed out, a new channel formed and the river bed was very much broadened. The first step in the construction of the new barrier was the laying of four heavy log stringers across this new channel from the abutment on the north to BuL. U. S. B. F., 190S. Plate LXXXVI. Fig. 19. -Main rack across Battle Creek near the Battle Creek station, California. Upper sections of rack raised to facilitate disposal of leaves and other debris. Fig. 20.— Seining spawning almon on the McCloud River, California, at the Baird station, replaced the hand windlass. Steam power has now FISH-CULTURAL PRACTICES IN THE BUREAU OF FISHERIES. 729 the new bank on the south side of the stream. The logs were let down through the dam foundation to low-water level on the north side and the deep charmel under them on the south side was filled with brush and gravel. The logs were spotted down to form a practically level bed, reaching the width of the stream. Heavy piles were then driven Ijehind each stringer to fonn alternate single and double rows extending up and down stream. The log stringers were next planked over, forming a platform 18 feet wide, similar to a regular dam apron, extending from the north abutment to the final row of piles on the south side, a distance of about 140 feet. By planking the sides of the single rows of piles and all around the double rows and filling the space with rocks, piers 4 feet high and approximately 2 feet and 4 feet wide were formed. Through each pier at the bottom, behind the upstream pile, openings i foot square were left, connecting the spaces between the piers. These spaces, 12 in number, are approximately 8 feet wide and are filled by swinging gates hinged to a 3 by 12 inch timber, spiked securely to the piers on either side and forming a dam or flashboard across the space above. By the insertion of other flashboards above this one a tight dam 4 feet high can be quickly formed at any time. The utility of this feature will be explained elsewhere. The gates are made of i by 4 inch fir set on edge and nailed to 2 by 4 inch joist, being strengthened by 2-inch blocks set between the rack bars and nailed to them and the joists. These blocks thus determine the width of the inter- stices in the gates. At the upper end of each gate an auger hole is bored through the bars and blocks, to accommodate a 2-inch iron pipe, which passes through the entire upper end of the gates. Ringbolts clasp these pipes and are fastened to the 3 by 12 inch timber forming the flashboard, acting as hinges upon which the gates swing. At the lower end of each gate a wide board, i}4 hy 16 inches, is secured by means of braces, forming an angle of 45° with the lower end of the gate. At any ordinary stage of the stream the downstream ends of 'the gates rest on supports which hold them a foot or more higher than the upper ends, the water passing down through them to the floor of the apron, where it runs away. The fish working up under the gates to the dam board find the cross passages through the front end of the piers and finally reach the trap. It was expected that during freshets the current acting on the flashboard would always keep the lower ends of the gates above the surface of the water, and up to a certain point this expectation was realized, but at very high stages of the stream the large quantity of gravel in the water soon clogs and sinks the gates. As the gates are only two-thirds the length of the apron, however, and rise toward the lower end, the water shoots over them with such force that it is projected some distance below the end of the apron, and fish attempting to 730 BULLETIN OF THE BUREAU OF FISHERIES. u-S a: >-; !< Kj" § ^ n T3 C ^ K UJ « a 0) a c J •«-< 1 ^) & R VI •0 S rt rt •o m cc cq Q ui u; {£ J5 FISH-CULTURAL, PRACTICES IN THE BUREAU OF FISHERIES. 731 ¥ ^ ci t rt il '^ w V B I1 1 a r. bd c & c .- w y= 732 BULLETIxV OK THE BUREAU OF FISHERIES. scale the obstruction fall far. short of the ends of the gates. The barrier has been watched many times when fish were jumping and when the largest drift ran clear, and none has ever been seen to pass it. By means of the dam boards entire control of the current can be had during ordinary stages of water and any desired quantity sent to any section of the barrier. Thus a strong current can be maintained through the trap section, leading the fish to it, and when it is desired to remove the fish from the trap the water can practically all be turned to some other section of the barrier. One of the greatest difficulties in maintaining traps in the streams in this section is due to the tremendous quantities of gravel carried in the water during freshets, a sufficient amount being frequently deposited in front of a trap at such times to change the course of the stream. With the present form of barrier no trouble is experienced from this source, the insertion of the dam boards and the opening of one space at a time quickly clearing away the accumulated gravel. The ninth and tenth piers were continued upstream by driving three addi- tional piles above each. The piers form the sides of the trap. Its floor is a plank bottom, similar in construction to the apron, and the front is barred by i%'-inch pickets placed i^ inches apart, the fish entering by the usual upstream V of pickets. To protect the trap from high water the two piers between which it is located were carried to a height of 8 feet. When it is desired to fish the trap, the gate at its head is closed and entrance is made from below by means of a door in the north side of the V. The upper end of the fishway of the old dam was left in place, the narrow passage between it and the new trap protecting the spaces at the south end of the barrier from the current and from drift. These spaces have been racked above and below to form commodious pens for males and unripe females. The south end of the barrier is protected by a substantial abutment. The maintenance of racks in Phinney Creek has been a very heavy item of expense in past years, and the trap was frequently carried away by freshets just at the height of the season, allowing large numbers of fish to escape and considerably reducing the season's take of eggs. It is believed the new barrier will stand any possible test that may be put upon it and will fish successfully in almost any stage of water. The design is to be credited to Mr. A. H. Dinsmore, superintendent of the station. TAKING AND HATCHING THE EGGS. Seining operations and spawntaking at California stations. — At the Baird station all the salmon are caught by seine with the exception of a very few taken in the trap in the upper rack. Owing to the swift current and the formation of the river banks the seine is always landed at one place, the rope attached to FISH-CULTURAL PRACTICES IN THE BUREAU OF FISHERIES. 733 the upstream end being hauled by steam engine and the lower or down- stream rope by a whim operated by horsepower. When a haul is to be made the seine is carried by boat upstream about 50 yards from the landing before it is paid out from the shore. The downstream rope is paid out automatically as the boat moves and is passed through a snatch block to keep the current from closing the ends of the seine until opposite the landing place, when both ends are drawn in at the same time. As soon as the seine has been laid out above the pool the engine is started, drawing the seine downstream toward the landing. When the last of the seine is in the water the whim is started and the lower end is also hauled down and in. A landing having been made, the ends of the seine are secured to pieces of rope fastened to pins driven into the ground. Hooks on loose ends of these ropes are slipped behind the corks of the seine. A trestle is placed under the rope to hold the cork line high above the water and thus prevent the salmon from leaping over it. The lead line is pulled upon the bank and thrown ovei iron pins to keep it from slipping back into the deep water. The ends having thus been fastened, all the men are free to handle the fish, which must be quickly removed to prevent injury from crowding, this being especially true of the summer run when the water is warm. The men stand in the water and lift the fish from the net one by one until all have been looked over, handling them with the aid of heavy woolen gloves, and grasping them by the tail with the left hand while the right is placed just behind the pectoral fins. A gentle pressure of the fingers at this point forces out a few eggs if the fish is ripe; if not ripe it is thrown over the seine into the river. When a ripe female is found it is carried to the pens beneath the spawning platform a few yards distant, care being taken to hold it vent side up to prevent loss of eggs. The males are handled in a similar manner, the ripe ones being placed in pens near the females. The seining crew are likewise the spawntakers, and after two hauls in the morning they strip the fish caught the day before. The ripe salmon of each day's catch are thus held over as a precaution against any possible immaturity of the eggs. On the spawning platform is a frame 6 or 8 inches wide, with sides con- verging toward the bottom and open at one end, into which a spawning pan can be slipped. The pan is rectangular in shape, about 6 inches wide and 14 inches long, with slanting sides and flaring ends. This shape is preferable to the round pan because of facility in washing the eggs, it being possible to dip a thin stream of water into the pan the entire length of one side. The frame holds the pan secure and at the same time its slanting sides assist in guiding the eggs into the pan. After washing, the eggs are placed in 6-gallon spawn buckets which have previously been filled with water. These buckets are made of galvanized iron, with a wire-cloth strainer inserted near the rim to permit the escape of surplus 734 BULLETIN OF THE BUREAU OF FISHERIES. water as eggs are added; they are painted inside with asphaltum, and are provided with covers. With the eggs in them they are placed on a platform which is built under water at sufficient depth to submerge about two-thirds of the body of the bucket, and thus maintain a proper temperature durin- water hardening. It is an essential feature of this platform that it be entirely independent of the spawning platform in construction. The period between the washing and hardening of the eggs is a critical one, and if they are jarred or disturbed there will be consequent greater loss in the hatchery. In the spawntaking operations five or six male fish are first dipped from the pen and dropped on the floor, where they are allowed to lie until they stop struct ghng and may be more easily handled. Then several females are'dipped ou^t killed by a blow on the head with a piece of iron piping, and laid in the dead box. The bottom of this box is inclined and has a narrow sht at the lower end, through which any eggs that may escape from the fish will fall into a pan underneath, where they will be fertilized by occasional applications of milt The dipping and killing continues until all the fish are stripped, males always being kept on the floor in order to have several ahead of the spawntakers After being stripped the best-appearing males are returned to the pens, where they usually recuperate and are again used to supply milt. The female fish is grasped by forcing the fingers through the gills and the thumb into the mouth, the hand being protected by a stout leather glove The man who does this, the headholder, stands, holding the fish vertically. The spawntaker, in a kneeling position, seizes the fish by the tail, bending its body over the pan, while with a sweeping motion he makes an incision in the thin side of the belly beginning near the pectoral fins and extending to the anal The incision is usually made with a pocketknife, the blade being held between the thumb and index finger within one-half to three-fourths inch of its extreme point to prevent cutting too deep. Most of the eggs follow the knife and fall into the pan, the remaining ripe eggs being released by running the fingers into the body cavity. As soon as the eggs begin to flow into the pan milt is forced over them and they are stirred by the spawntaker with a few movements of the hand. They are then passed to the washer. The milt of one male may serve for several pans of eggs, but it often happens early in the season that several males are required to one pan. As soon as the washer receives the pan of eggs he dips the edge of it in the river, the inflowing water causing the eggs and milt to boil up. The eggs at once settle back and the milt is poured off over the side of the pan. Ordinarily two such dips suffice to clean the eggs, after which they are poured into spawn buckets, and at once settle to the bottom, the surplus water escaping through the wire-cloth strainer near the top. Spawning operations usually consume about an hour. After water hardening the eggs are carried to the hatchery, about 200 yards distant, and turned over to the hatchery crew. BUL. U. S. B. F., iqoS. Plate LXXXVII. Fig. 21. — Spawiitaking operations. Baird. Cal. The fish (chinook salmon) are dipped Irom the pen, killed by a blow on the head, and passed to the spawntakers. The eggs are taken by opening the abdomen, and the stream of eggs may be seen in the picture following the hand making the incision. Fig. 22.— One method of stripping steelhead trout. Since this fish normally does not die after spawning, the ripe fish are not killed as are the salmon, and they are so large, and so powerful in their strug- gles, that the strait-jacket here shown is sometimes resorted to. FISH-CULTURAL PRACTICES IN THE BUREAU OF FISHERIES. 735 The spawning operations at Battle Creek and Mill Creek are practically the same as those at Baird, and the seining differs only in minor details to meet local conditions. The method of taking eggs by incision has many advantages over the older practice of expelling by hand, chief among them being the larger number of good eggs secured and the higher percentage of impregnation. There is also a great saving of time and labor, and, as all of the Pacific salmons die after once spawn- ing, the killing of them in the process of taking the eggs merely hastens the end, and constitutes no loss of adult fish. The eggs of the Pacific salmons are comparatively large, ranging in diameter from one-fourth inch for chinook to one-fifth inch for sockeye. Fortunately, however, these large eggs do not require the same treatment and care as do those of the other Salmonidae, but may be placed 12 to 20 deep in wire-cloth baskets, 30,000 to 50,000 to the basket, water temperature being an important factor in deciding the quantity. The baskets, rectangular in shape, conform in width to the troughs; the latter are of the Williamson type (up-current), the flow of water to each trough varying with local conditions at the different hatcheries from 8 to 20 gallons per minute. Local practices in different regions. — Experience seems to indicate, with regard to the sockeye, or red salmon, that there is an advantage in bleeding the fish before stripping, thus obviating a flow of blood with the eggs when the incision is made. It is therefore now customary at sockeye stations to decapi- tate or else cut off the tails of the female fish as the first step in the spawn- taking process. At Baker Lake, Washington, the practice is as follows: The fish, which have been brought to the pens usually the da}- previous, are dipped out one by one, decapitated, and dropped upon a draining rack, where water is thrown over them to cleanse them for handling by the spawn- taker. The latter impales the fish on a short spike conveniently located to hold them over the pan while he makes the incision and removes the eggs. Two men are occupied in the work so far. A third fertilizes and washes the eggs, then conveys them to the hatchery. A million eggs may be secured in this manner in one forenoon b)' one such crew. Although not suitable for canning or for the market, the sockeye at this period is edible and many are taken by local residents for food. Indians camp at some of the stations and preserve large numbers of the salmon. The majority of those killed, however, go to waste. At the Battle Creek station in one season it is not an unusual thing to btiry during the spawning season 15,000 to 20,000 pounds of fish. A convenient and economical method of separating dead eggs of salmon from living ones is the use of a salt solution." If a basket of eggs is emptied "O'Malley, Henry: Salt solution as an aid to fish culture. Transactions of the American Fish- eries Society for 1905, p. 49. 736 BULLETIN OF THE BUREAU OF FISHERIES. into the solution, the unfertihzed and dead eggs rise to the surface, where they may be quickly skimmed off. The method is not applicable in the manipulation of small quantities of eggs, but is very useful when, for some cause, there is an abnormal loss. It has not been successfully extended to the eggs of species other than the chinook, silver, and humpback salmons. At the Baker Lake station, operated primarily for the propagation of the sockeye salmon, both sockeye and silver salmon are captured by means of a pound net as they enter the lake, but as the fish are not ripe at this stage of their upstream passage it is necessary to hold them until the spawning season. For this purpose a slough or bay at the head of the lake has been inclosed by racks and webbing to make a pound about 20 feet wide and 500 feet long, with an average depth of 6 feet. The entire bottom area is of soft mud ; in it are two hollows of a maximum surface area of 300 feet and 400 feet, respectively, where the water is approximately 12 feet in depth. The water supply, from 800 to 2,400 gallons per minute, is derived from several small mountain creeks fed by glaciers and snow, and from springs. This has proved an ideal place in which to hold salmon while their eggs are maturing. As many as 8,000 fish have thus been confined for thirty days and 6,000 for three months. After remaining in the inclosure for three or four months the fish are as clean and free from abrasion as fish that have not been penned. A noticeable fact, however, is that very few of these salmon ripen until October or November, while in previous years the spawning season has oc- curred during September and before October 15. The difference is attributed to the low temperature of the water in the inclosure, which remains from 6 to 8 degrees below that of the natural spawning beds of the lake. (During the sum- mer the average water temperature on the spawning beds is about 56°.) The success of the impounding of the salmon is attributed to the low temperature of the water. Silver salmon, as well as sockeye, have been successfully impounded at Baker Lake. A few impounded chinook, however — never more than 12 at a time — fungussed rapidly, and after three weeks usually died before the eggs were taken. MARINE FISH CULTURE. COD, POLLOCK, AND FLATFISH. On the New England coast the Bureau maintains three stations, for the hatching of marine fishes and the lobster. Of the fishes the cod is first in importance. Its cultivation consists prin- cipally in hatching eggs obtained from market fish, the fish being stripped either by the fishermen or by the Bureau's spawntakers. The latter are daily distrib- uted among the fishing vessels for the double purpose of stripping ripe fish as fast as hauled aboard and of collecting and caring for all eggs the fishermen may FISH-CULTURAL PRACTICES IN THE BUREAU OF FISHERIES. 737 have taken. Coming as it does during the winter months, from early December to March on the Massachusetts coast and extending through March and April on the Maine coast, the spawning season is a trying one for the spawntakers, who must share many of the dangers and hardships of the fishermen. At the Woods Hole station advantage has been taken of the presence of a large salt-water reservoir under the hatchery to test the Norwegian method of obtaining eggs. By this process adult fish are penned and allowed to spawn naturally. The eggs float to the outlet, where they are caught in a receptacle placed for the purpose, and thence are transferred to the hatching boxes. It has been demonstrated that a larger percentage of fertilized eggs per fish can be obtained thus than by the former method of stripping penned fish. The increase is not due to a higher percentage in fertilization, but to the fact that in the case of the penned fish there is an almost unavoidable loss of eggs extruded in the crates. Both of these methods are an improvement over nature, in that the eggs are protected from the time of extrusion until they have hatched and the surviving parent fish are returned to the ocean. This is fish culture in the usual sense and not purely conservation of an otherwise waste product, as is the collection of eggs from market fish. Prior to the spawning season for cod, or from the middle of October to the latter part of December, spawntakers are distributed among the pollock fisher- men, and from the eggs thus collected many millions of pollock fry are hatched and distributed annually. The cultivation of flatfish is conducted on a more extensive scale than any other marine fish-culture work. The adult fish are taken from the fyke nets in which captured, usually the Bureau's own, directly to the hatcheries, where they are placed in tanks and held until they have spawned, the eggs being removed daily from the tanks to the hatching apparatus. It is possible to spawn flatfish artificially, and eggs are sometimes obtained in that way. LOBSTERS. Lobster culture also, as conducted by the Bureau, effects a saving of an other- wise lost resource, berried lobsters purchased from the fishermen furnishing eggs for the hatchery and being later retvu-ned to the ocean. At the Boothbay Har- bor, Me., station the parent lobsters are held until the eggs are ripe in a pound similar to those used by the lobster men ; and as it has been found that eggs taken late in the fall or early winter do not hatch successfully, one such pound is utilized to hold some 10,000 or 12,000 lobsters throughout the winter. The losses of lobsters during the period of confinement are only normal, and the quantity and quality of the eggs are superior to those obtained from freshly caught stock. It is noticeable also that the eggs of the impounded stock hatch almost simul- taneously and somewhat earlier than those from freshly caught lobsters, undoubt- B. B. F. 1908 — 47 738 BULIvETIN OF THE BUREAU OF FISHERIES. edly bee use of the warmer, shallow, inshore water. By the middle of April the eggs are sufficiently well advanced to be removed from the parent and put up in the hatching jars; and as at this time the lobsters become quite active in a rising water temperature it is quite important that they then be removed to avoid serious losses from mutilation. The removal of impounded lobsters is effected at low tide, the flood gates being opened and a portion of the water drawn off with care to retain enough water to protect the lobsters from exposure. Men in dories then go about the shallow portions of the pound picking up the lobsters on ordinary clam forks or hoes, the sharp teeth of which have been blunted. It was formerly the custom to use a drag seine for gathering the lobsters, but taken in such quantities they mutilate one another and it has been found preferable to remove them by hand. After a portion of the stock has been removed the water is drawn still lower, until finally only a small area of the pound is flooded, and the remaining lobsters are removed. As it has not been possible to transport lobster eggs successfully when detached, the berried females are always taken to the hatchery to be stripped, the transfer being made in the wells of fishing smacks or the Bureau's vessels. From this time to the close of the season in July berried lobsters are collected from the fishermen and transferred to the hatchery to be stripped. Imme- diately after the close of the season the collection of fresh berried lobsters for stocking the pound is begun and continued into November. It is unquestionable that the impounding of lobsters as practiced in Maine is superior to any other present method of holding the adults for a length of time. The character of the Maine coast, with its numerous natural inlets and its unusual rise and fall of tide, affords especial advantages for the use of pounds. When these conditions do not exist, however, recourse can be had to cars, although data thus far obtainable fully demonstrate that a larger number of lobsters can be held for a longer time and with a smaller percentage of loss in pounds than in cars. Contrary to the custom of the pound fishermen, the ice on the surface of the pound operated by the Bureau of Fisheries is removed from time to time during the winter, with the result that a much larger percentage of lobsters is found in the spring. It has been observed that the rising and falling of ice with the tide frequently crushes lobsters that happen to be in the shallow water near the edges of the pound, and removal of the ice at intervals obviates this difficulty. Experiments have been made as to the poimdkeepers' practice of inserting wooden plugs in the claws of penned lobsters to prevent their mutilating one another. For lobsters intended for market the procedure seems suitable, though it results in an unsightly discoloration of the muscles. There seemed BuL. U. S. B. F., 1908. PI.ATE LXXXVIII. HiG. 23. — Eiliiipiiient of IMcDuiiakl ;iiitonicitic tidal buxtr>. fur hatching cod, liootlibay Harliur. ^Ic. Shuw:^ buxcb lifted out of troughs and bottom upward on farther tables. The bottom is of scrim, and by means of cleats is held i^ inches above the bottom of the trough at the center. By an arrangement of partitions at the head of the trough the eggs receive the supply of water through the scrim bottoms of the boxes, also through a small hole in one end of the box. The distinctive feature of the apparatus, whence it is called " tidal," is the automatic siphon outflow, by means of which the water is alternately drawn down and replenished. Staudpipe with siphon cap is shown in near troughs; waste trough below. Fig. 24.— Beri-ied lobsters, taken fn.in pMund .ii l;. m ,iii!,,,y ILirh.i. -tUiuu iMaine). in course of transfer to wells of the steamer which is to couvey them to the hatchery for stripping. FISH-CULTURAL PRACTICES IN THE BUREAU OF FISHERIES. 739 to be a question, however, whether it would be feasible with the impounded stock of the Bureau destined for liberation in the open waters after removal of their eggs. The experiments showed that out of 2,110 lobsters removed from the pound, all of which had been plugged when put in, 742 had lost both plugs, 563 had lost one plug, and 605 retained both. The warm weather when they are first confined, however, is their most active period, when the plugs do most good. Mutilation is thus prevented and the plugs apparently work no per- manent injury to the lobsters. It is important in the impounding of lobsters to take precautions, so far as possible, for the exclusion of eels, which have an especial liking for the eggs and will strip a female lobster in a very short time. Even with all precautions it seems impossible to exclude eels entirely ; it is probable that many enter when small and grow up in the pound. The rearing of lobster fry to the fourth molt, as practiced by the Rhode Island Fish Commission and so admirably set forth in a paper read at the con- gress," has not as yet been taken up by the Bureau, but is doubtless feasible at the Boothbay Harbor station. Before the first experiments in this direction at Boothbay it was thought that owing to the lower temperature of the water in this more northern latitude the periods of molting would be prolonged and the feeding and care of the fry consequently attended with abnormal losses. It has since been found that this difficulty can probably be met by installing the rearing plant in a lobster pound, where the temperature is higher and more even than in the open waters. The Bureau therefore hopes to enter upon this under- taking in the near future, for the purpose of rearing a portion of the lobster out- put. To attempt to rear to the fourth molt the entire product of the Boothbay station would involve an expenditure far beyond present financial resources. MEASURING AND COUNTING HSH EGGS AND FRY. Immediately after water hardening, for a short period varying with the species and water temperature, the careful handling of fish eggs is not injurious. During this period their numbers may be very definitely ascertained by the use of any receptacle suitable for a measure, the capacity of the receptacle having first been ascertained by counting the whole or a fractional part of its contents. For eggs of the trouts and those of smaller size an apothecary's graduate or the ordinary graduated quart or pint measure is commonly used. For large quantities the long-handled dipper used in transferring them to the hatching apparatus may be advantageously utilized. As many eggs as possible are poured into the measure, nearly all of the water being forced out over the rim. "Mead, A. D.: A method of lobster culture. Proceedings Fourth International Fishery Congress, Bulletin of the Bureau of Fisheries, vol. x.xviii, 1908, p. 219-240, pi. vii-xi. 740 BULLETIN OF THE BUREAU OF FISHERIES. Unless the eggs are to be transferred to a hatchery beyond the jurisdiction of the shipper, the eggs may not be measured until a more convenient time, it being possible from long familiarity with the capacity of the apparatus in actual use to estimate quite accurately the number of eggs on hand at any time. Pro- viding they are spread uniformly, the number of eggs to a square inch is a fairly accurate basis for ascertaining how many eggs are on each tray. Some fish culturists prefer to ascertain the actual number of eggs on hand by weighing them after having determined by actual count the basis for such calculations. These methods are especially applicable to the heavy eggs of the Salmonidge, and may be employed not only after water hardening but also at any stage of incubation after the eggs are eyed up to a day or so before hatching, at which last stage a measurement closely approximates the number of fry that will be in the subsequent hatch. Eggs hatched in jars are usually measured by means of a graduated scale in the form of a square made of wood, the units indicated on the long leg of the square. The square is adjusted to the jar as shown in figure i. The scale reads from the bottom line upward, the first or bottom line being at a height corre- sponding to the level attained in a jar by a measured half pint of water, and each line represents the number of eggs of a given species as established by actual count from a measured half pint. The dead eggs will have been from time to time siphoned oif and when the remainder are fully developed or about to hatch the scale is applied to each jar and a very careful measurement is made to ascer- tain their number. The number of fry available for distribution will be approxi- mately the number of eyed eggs in the jars just before hatching, as the mortality after this stage of development is usually inappreciable. A novel method of obtaining the number of eggs in a given lot has lately come into use, and as the work can be done without counting a large number of eggs, has proved especially valuable in dealing with eggs of small size at field stations where no measurements have been established. This method, devised by Mr. H. von Bayer, architect and engineer of the Btueau, employs a gauge which quickly gives the diameter of the eggs, knowing which it is possible by reference to a diagram to determine at once the number of eggs to the quart." In the keeping of accurate hatching records it is important that the basis for computations be ascertained immediately before any general measuring methods are applied because it is well recognized that eggs of most species vary in diameter with stock from different waters and that eggs from any given col- lecting station vary at different periods of the spawning season, those taken at the height of the season being larger and more uniform than those taken earlier or toward the close of the season. For instance, brook trout eggs taken at a " von Bayer, H. ; A method of measuring fish eggs Proceedings Fourth International Fishery Congress, Bulletin of the Bureau of Fisheries, vol. xxviii, 1908, p. 1009-1014. FISH-CUI-:;•/''; ^•■ ' / .K '.VvM^./A Xi^tycTeoF' '4 Fig. 7- — Atkins-Dinsmore shipping case. Longitudinal section -z;^ »3- -J^ X ^ 1 ■.s^ «^1 \ A lyi^ F^ V fi Fig. 8. — Atkins-Dinsmore shipping case. Plan. FISH-CULTURAL PRACTICES IN THE BUREAU OF FISHERIES. 745 Aaja i-l. •-, :i, ' } — ^^ — " ||h .-'3- J V :£ -t3 — rr iht'n^fs purpose they are laid in cheese cloth on wire-bottom trays, between wooden frames also covered with wire cloth, strapped together, and shipped without further packing. Experiments at Havre de Grace, Md., seem to demonstrate that it is prac- ticable to pack eggs of the white perch in the ordinary trout egg case with ice hopper and ship them on journeys of thirty-six hours' duration without apparent injury. Two lots of green eggs taken at a temperature of 56° and 70°, respec- tively, and held in trout egg cases for twenty-four to twenty-six hours, hatched as well as eggs placed directly in the hatching jars. In the first experiment there was no change in temperature, but in the second experiment there was a fall from 70° to 64°. White perch eggs to the number of 3,000,000 have in several instances been .^i"*" shipped by express from Havre de Grace, Md., to Washington, D. C, a half day's travel, in four McDon- ald jars packed in sawdust, ice being used in the packing when the air temperature seemed to require. The jars were equipped with the usual glass tubes, which extended above the packing, but whether this provision for aeration was necessary has not been tested. To in- sure proper aeration, how- ever, it would seem advis- able, with present knowl- edge of the subject, not to ship large numbers of white perch eggs in water for travel of four or more hours without a caretaker. Attempts to transport yellow perch eggs on trays have not given satisfac- tory results, but it is apparently possible to carry them successfully almost any reasonable distance in the ordinary transportation cans, i to 2 gallons of eggs to 8 gallons of water, the proportion varying with the distance to be traveled, and care being taken to aerate and temper the water. Green pike perch eggs may be carried from near-by collecting grounds to the hatchery in tubs or transportation cans, care being taken to renew the water frequently, to keep it well aerated, and of a proper temperature. Ice must be prevented from coming in contact ,vith the eggs, because, unlike most Fig. 9. -Alkius-Diusmure shipping case Cross section. 746 BULLETIN OF THE BUREAU OF FISHERIES. other eggs, they are very sensitive to such exposure. Half-barrel fish kegs or kits with one end knocked out and iron handles attached make very good and economical vessels in which to transport pike perch eggs. It is customary to insert a wire-cloth drain in the top of the kit on one side and the kits are asphalted inside. Canvas is thrown over the top to serve as a cover. When the eggs are held over night in these kits it is expected to supply them with running water whenever possible to do so ; otherwise the water must be period- ically renewed or aerated. At some of the field stations a pipe arranged with pet cocks and rubber tubing for supplying water to a number of kits or trans- portation cans saves much labor in the matter of aerating eggs which must be held for a day or more. Pike perch eggs collected at a distance from the hatchery are conveyed thereto in the usual transportation case on trays, a small amount of ice being placed on the top tray, which is substituted for the usual ice hopper; an inch space around the sides of the stack is also filled with ice. Ice over the stack must be used sparingly or the green eggs may be injured by the cold water where it trickles upon them. For large shipments it is customary to have a caretaker accompany the packages to regulate the temperature, etc., the use of moss on the trays as well as the insulation material then being omitted. Since eyed pike perch eggs are usually shipped during the month of May, for safety ice is used around the stack of trays as well as in the hopper, even on a two days' journey. Whitefish eggs are transported from the field of collection by both of the methods employed in the transfer of pike perch eggs, although they do not require quite so much attention. Eyed whitefish eggs are packed on trays in the ordinary way. The fields for the collection of lake trout eggs being widely distributed, it sometimes happens that green eggs are held in transportation cases for several days before their arrival at the hatchery. They carry as well laid directly on the wire-cloth bottoms as on a layer of cheese cloth. No packing is necessary if they are in the care of an attendant, since the latter can regulate the temper- ature by the use of ice and with water of the proper temperature, the trays being removed and sprinkled at least once in twenty-four hours. In packing lake trout eggs for short distances, say up to a thousand miles, the ice hopper is omitted. Mosquito netting and moss are put on in the usual manner and the top of the moss is slightly frosted before the trays are stacked on the baseboard. A light piece of lumber is used in place of the ice hopper and fine shavings are solidly packed under, above, and on all sides of the stack of trays. For the longer shipments the ordinary ice hopper is used, fine shav- ings being placed around the stack of trays for insulation. FISH-CULTURAL PRACTICES IN THE BUREAU OF FISHERIES. 747 In the handling of eggs of the cod and other marine fishes a so-called kettle, oval and with a concave top, is used to retain them on board ship in a choppy sea. The water in which they are kept must be frequently changed or aerated. The eggs are shipped to the hatchery in large fruit or butter jars, rockweed or moss, together with ice or snow, being used in packing them. It is regarded as impracticable to ship eggs of marine fishes for travel of more than two or three days. ARGENTINE CASE. While the various methods above described have all been successfully employed in the transportation of eggs across the United States and also to Europe without an attendant, shipments of eggs to points south of the equator, usually leaving this country in winter and arriving at their destination in sum- mer, have called for more than usual attention to the methods of packing them, and a caretaker is quite essential. A highly efficient form of shipping case has been developed during the past few years for the transportation of eggs of the Salmonidee from this country to Argentina. It is 3 feet 6 inches long, 2 feet wide, and not exceeding 30 inches high, outside measurement, and is constructed of selected tongued and grooved lumber. It has double walls, with bottom and top common to both, the 2-inch space between the walls being filled with nonconducting material, preferably tightly packed shavings. Between the inner wall and the stack of trays is a 2i'4^-inch space for ice, separated from the trays by perforated zinc. Between the latter and the trays, in a 3^-inch space, are the vertical supports of the zinc, viz, double corner supports, one being ^ by iX inches, the other being % by i inch; two intermediate supports of ^ by i inch material, which are provided on either side of the case and one at each end; and cross braces of X by I inch material, which extend from the uprights to the inner walls of the case. The ice hopper, 3 inches in depth, and having the same outside dimen- sions as the trays, rests upon the latter and fills the space between the upper- most tray and the top of the case. It has a perforated zinc bottom, and, to facilitate handling, cleats of small ropes are attached to it. The top of the case is insulated with a 2 -inch thickness of nonconductor covered with sheet zinc, this insulation fitting closely into the chest when closed, and thus covering not only the ice hopper but the ice spaces around the sides as well. In the bottom grooves lead to a %'-inch drain hole, which is provided with a cork. Two cleats li, by 3 inches are attached lengthwise to the bottom on the outside. The trays are one-half inch deep, 27 inches long, and 9 inches wide inside measurements, the frames being of yi by X inch material. The bottom of each 748 BULLETIN OF THE BUREAU OF FISHERIES. tray is covered with wire cloth no. 25 gauge, about 12 meshes to the inch, stretched tightly to prevent sagging and consequent uneven distribution of the drip water. A narrow binding of cloth is tacked around the bottom of each tray to prevent the wire edge from catching on the mosquito net covering of the tray beneath. On the inside ends of the traj^s are fastened short lifting cleats, and wedges hold the trays securely in place. The bottom tray rests on three X"inch cleats extending lengthwise of the case, one at either side and the other in the middle. It is important to have the trays of uniform size, that they may be interchangeable. The trays and interior of the case are coated with asphaltum. To facili- tate opening from either side, four hasps are used, two on each side of the case. Two rope handles side by side are placed on each end of the case, with a cleat of three -fourth inch material just above the holes for each handle. /■/ra /a trji^ff Fig. 10. — Argentine shipping case. Section. Eggs selected for shipping should barely show the eye spots without the aid of a glass. In packing, a layer of damp moss is spread one-fourth of an inch deep as evenly as possible over the tray bottom, and upon this is placed a covering of mosquito net or bobbinet. The eggs are laid upon the netting one or two layers deep, spread to within one-half inch of the tray frame and covered with another piece of netting to keep them separate from the moss, which is sprinkled in a light layer over it, filling the tray. The netting is cut large enough to extend over the outer edges of the tray, so that the eggs may not be disturbed when a tray is lifted for examination. On shipboard, as the greater part of the journey is made, the cases of eggs are kept in one of the fruit or cold storage rooms having a temperature of about 38° F. To this room the attendant has access, and it is his duty daily to moisten FISH-CULTURAL PRACTICES IN THE BUREAU OF FISHERIES. 749 the eggs by pouring through the ice hopper water of the same temperature as the eggs, 34° to 35°. The ice compartments are frequently replenished and the eggs are picked over whenever necessary. ,4^^ s «». It will be seen that the method of caring for the eggs is not novel. The chief improvements in the case are to make it easy for the caretaker to tend the eggs in the crowded quarters of a ship's storage compartments and to facil- itate handling each individual tray. 750 BUI^LETIN OF THE BUREAU OF FISHERIES. GERMAN-CHILE CASE. Another case used on journeys in which the care of the eggs is the same as above described is the German-Chile case, so called by reason of having first been employed by German fish culturists in shipping trout eggs from Germany to the Chilean Government. It was brought to the attention of fish culturists in this country by Mr. E. A. Tulian, chief of the section of fish culture of the Argentine Government. In 1907 and 1908 this case, somewhat modified, was used by Mr. Tulian in transporting trout and salmon eggs from the United States to Argentina with better results than had been secured with any other form of case. Owing to the absence of moss directly on the eggs, the German- Chile case is especially adapted to the handling of rainbow trout eggs, the mem- brane of which is more delicate than that of any other species of the Salmonidae. From the latest observations it is undoubtedly true that the ideal form of long- distance shipping case for all species, at least when accompanied by an attend- ant, is the one wherein no moss or other substance is placed directlj' on the eggs. The German-Chile case is constructed on the same principles as the Argen- tine case above described. The case proper is built of selected lumber and is 2()yi inches long, 20 inches wide, and 15 inches high, outside measurements. It is so similar in construction to the Argentine case that, aside from a general description, only the differences between the two will be pointed out. The German-Chile case accommodates two stacks of trays 7K t>y 8^ inches, in a double-chambered compartment having walls of unperforated galvanized iron, which is strengthened by a heavy wire around the top edge. A removable metal partition separates the two stacks of trays. The compartment itself is made one-fourth inch larger each way than the tray frames, to allow for swelling and the binding twine which will be placed around the trays. Next to and surrounding the tray compartment is the ice, outside of which is the dry moss or other nonconductor, within wooden walls, the same as in the Argentine case, while resting upon the top of the trays and ice compartments is an ice hopper. For purposes of insulation the ice in the hopper is covered with a cushion filled with dry moss, oilcloth being placed between the cushion and the ice. The metal bottom of the hopper has perforations only over the trays, that the eggs may receive the benefit of all drip water. Small cleats are fixed at either end of the ice hopper to facilitate handling. Under the tray compartment and coextensive with it is a perforated wooden false bottom to the case, between which and the bottom proper is a i-inch air space. A drain hole is provided in the bottom proper. The egg trays are made a trifle deeper than the diameter of the eggs; and the latter are placed on them a single layer deep without any FISH-CUWURAL PRACTICES IN THE BUREAU OF FISHERIES. 751 covering of moss. The tray frames are seven-eighths inch wide, and usuall}" three-sixteenths inch to five-sixteenths inch thick, with a bottom either scrim Fig. 12. — German-Chile shipping case. Longitudinal section. or canton flannel. Instead of being made into one package for each chamber, the trays are bomid together in fives, with strong twine for binding material, and alternating with each package are double-depth trays, or dummies, filled Fig. 13. — German-Chile shipping case. Plan. with wet moss. One of these moss-filled trays is placed also in the bottom of each of the two tray chambers. 752 BULLETIN OF THE BUREAU OF FISHERIES. TRANSPORTATION OF FISH. In distributing young fish from the hatcheries to the waters they are to stock, six special cars are employed. They are equipped with all necessary apparatus for the safe carriage of young and adult fishes and each is provided with a buffet and sleeping accommodations for a crew of five men. The cars are attached to passenger trains, and many of the railroad companies, appre- ciating the benefits arising from stocking waters along their lines, haul the cars gratis; others make special rates for cars and crew. When plants are made off the main railroad lines, the fish are carried in baggage cars in charge of members of the car crew. In addition, distributions are made from many stations without the aid of the special cars, the station employees caring for the fish in baggage cars. For this service the railroad companies usually charge regular fare for the attendants but transport the fish and return the empty cans gratis. Fry and young fish are usually transported in lo-gallon round-shouldered iron cans, tinned for fresh-water work and galvanized for marine work. With fresh water galvanized iron often proves toxic, and should never be used in the transportation of fresh-water fishes. On the cars it is possible to aerate the water in each fish can by air pumped through a reservoir, from which it is taken through lines of piping along the sides of the car. The piping is equipped with pet cocks, from which the air is carried to the cans in 5 s-inch rubber tubing and forced into the water through liberators made of porous wood, preferably the American linden {Tilia americana) , placed in hard rubber holders. For best results these liberators must not be placed in the water until air pressure is on and must be removed when air pressure is stopped. In putting up cod fry and fry of other marine fishes for shipment it is cus- tomary to have several quarts of water in the transportation cans and then carefully dip the fry from the hatching boxes, lowering the dipper to the water in the cans before emptying it. When the box is nearly empty the remaining fry are removed from it to a can by means of a siphon. When transported on a vessel having a conveniently arranged well, linen scrim is securely fastened over the top of each can containing fry and the cans are laid horizontally in the well, the top toward a perforated supply pipe through which water is pumped into the well, thus maintaining a constant current. Lobster fry may be carried in scrim- walled containers, or boxes, suspended in the well of a vessel, the motion of the vessel and the constant circulation of water in the well keeping the fry in good condition and preventing their settling in a mass at the bottom. The boxes are made of a framework covered on the four sides and bottom with scrim, which allows a free circulation from all sides. Each box, 42 inches by 29 inches by 29 inches, will hold from 2,000,000 to FISH-CULTURAL PRACTICES IN THE BUREAU OF FISHERIES. 753 3,000,000 fry. It is customary to take from 12 to 15 cans of fry in addition to those taken in the well, the fry in the cans being the first planted. On vessels having no wells, aeration for all species of the marine fry, includ- ing the lobsters, is accomplished by siphoning off and dipping the water freely, as will be described for whitefish, pike perch, etc. For tempering the water ice is suspended from the cover of the can in a cylinder. Temperature is a most important consideration in the transportation of fishes, and owing to the different conditions under which they are hatched in the various localities, is a feature that requires skilled discretion on the part of the attendant. The general rule is to keep the temperature at least as low as that of the water from which the fish were taken, and lower if the species is not sensitive to changes. The maximum number of fishes to be carried most advantageously in a lo-gallon can is another equally important question. The distance to be trav- eled partly prescribes this number, but must be considered also with reference to the temperature; and these factors, interdependent as they are, go to prevent the formulation of any hard and fast rule. In the following table, however, attempt is made to generalize by means of average conditions, and show the number of fishes of specified kind and size as ordinarily transported in a 10- gallon can. The cooler period of the year in which handled accounts for the lower temperature for fry of the trouts as compared with the higher temperature of fingerlings. It also accounts for the lower temperature for landlocked salmon fingerlings as compared with fry. Ordinarily if landlocked salmon, rainbow trout, and brook trout fry from the same source should be distributed in warm weather it would be desirable to reduce the water temperature for the brook trout considerably lower than for the other two species. Number op Fishes oV Given Kinds and Sizes to be Transported in a io-Gallon Can under Average Conditions. Fry. Advanced fry. Fingerlings no. i. Fingerlings no. 2. Species. Number. .Tempera- ture. Number. Tempera- ture. Number. Tempera- ture. Number. Tempera- ture. Large-mouth and small- mouth black bass Rock bass, bream, and 3. 000 ' F. 60 1, 000 " F. 6! 400 400 I, 500 1.500 I, 2O0 I. 500 62 62 so 48 S3 44 225 225 500 500 500 500 " F. 63 63 so so 55 SO S.ooo S. 000 5.000 4. 000 2. 000 2, 000 30, 000 100, 000 125. 000 200, 000 30. 000 300.000 I, 000. 000 400. 000 100. 000 40 48 48 40 45 48 38 60 50 S3 68 42 43 3. 000 3.000 1. 500 3. 000 I, 000 I, 000 45 48 SO 42 48 48 Blackspotted trout f ^nHIocWprt «;almnn 400 44 Whitefisb 200 60 White perch Shad Cod . --- Flatfish Pollock S8 B. B. F. 1908 — 48 754 BULLETIN OF THE BUREAU OF FISHERIES. Number of Fishes of Given Kinds and Sizes to be Transported in a io-Gallon Can under Average Conditions — Continued. Fingerhngs no. 3. Fingerlings no. 4. Fingerlings no. 5. Fingerlings no. 6. Species. Number. Tempera- ture, Number. Tempera- ture. Number. Tempera- ture. Number. Tempera- ture. Large-mouth and small- mouth black bass Rock bass, bream, and ISO 150 250 200 150 ISO ° F. 63 63 SI 55 44 60 100 100 125 100 100 100 <■ F. 62 63 52 SS 44 60 50 50 61 61 30 30 ■> F. 61 61 75 54 Note. — The varying usage in the classification of young fish as to size has caused such confusion and difficulty that the Bureau has adopted uniform definitions, as follows: Fry = iish up to the time the yolk sac is absorbed and feeding begins. Advanced /ry = fish from the end of the fry period until they have reached a length of i inch. Fingerlings =&sh between the length of i inch and the yearling stage, the various sizes to be desig- nated as follows: No. 1, a fish i inch in length and up to 2 inches; no. 2, a fish 2 inches in length and up to 3 inches; no. 3, a fish 3 inches in length and up to 4 inches, etc. Yearlings=6sh that are i year old, but less than 2 years old from the date of hatching; these may be designated no. i, no. 2, no. 3, etc., after the plan prescribed for fingerlings. In the care of fish away from the air circulation of the cars, aeration is usually accomplished by the use of a long-handled dipper, and this method for 10 or 15 cans, which is the usual number handled by detached messengers, if energetically operated has no equal. Several forms of aerating devices have their good points, their use is permitted, and efforts are being made to improve upon the present methods of hand aeration in order to lighten the labors of the messengers, who not infrequently care for the fish for two or three days before they arrive at a destination. For aeration in cans containing small fry, such as the shad, pike perch, white perch, yellow perch, and whitefish, it is customary to siphon a portion of the water into a pail (the head of the siphon being in a wire cage covered with cheese cloth), aerate it with a dipper, add ice to temper it if necessary, and then pour it back through a large funnel which reaches nearly to the bottom of the can, the lower part of the funnel for about 6 inches being made of perforated tin to break the force of the water. On short trips little, if any, aeration is necessary. Cans holding the larger fry and young fish may be aerated by dipping the water up and pouring it back again from a height of 15 or 20 inches. When transportation is by water it is customary to renew the water in the cans en route instead of resorting to hand aeration. With fishes of the Salmonidae family, ice may be placed directly in the water in the cans. FISH-CULTURAL PRACTICES IN THE BUREAU OF FISHERIES. 755 DISTRIBUTION AND PLANTING OF THE COMMERCIAL FISHES. It being infeasible for various reasons to rear the young of the commercial fishes, practically all are planted as fry and the distributions are usually made by agents of the Bureau. Fry of the marine species are often transferred to small boats in order to plant in shallow water or they are carried from the hatchery in launches. In such cases the cans are lowered into the water by two men and there slowly inverted. Wlien planting from a vessel two lines are made fast to the can, one about the top and one about the bottom; by this means the can is lowered over the side and when partially submerged is emptied. On the Great Lakes the distributions of lake trout, whitefish, and pike perch are usually made by means of steam vessels. In such instances the water in the cans is renewed as often as necessary by siphoning off a portion and replenishing directly from the lake. The manner of liberating the fish after the point of deposit has been reached varies in practice. In planting lake trout and whitefish the method employed by the superintendent of the Duluth, Minn., station is to pour the water and fry, one can at a time, into a large tub full of water, from which the water and fish are siphoned through a heavy 2 -inch rubber hose attached to a pole or outrigger. Thus they are deposited in the lake about 8 feet from the side of the vessel, very close to if not beneath the surface of the lake, the speed of the vessel being slackened while the planting is in progress. As it is customary to transport the fry on passenger or package freight steamers, where they are necessarily stowed in a limited space and as close to one gangway as possible, the cans may be emptied into a tub sitting firmly on the deck more easily and expeditiously than they could be poured directly into the lake, and the element of danger to the men doing the work is avoided also. The superintendent of the Northville station follows a somewhat similar procedure in planting lake trout, pike perch, and whitefish fry. If the deck of the vessel is near the water surface a piece of ordinary tin pipe is attached to a tub and arranged with elbows so as to bring the lower end near the surface of the lake. If the deck of the vessel is high above the water the tub is used with 1 5 or 20 feet of 2-inch fire hose instead of the tin pipe, a weight being attached to the lower end. From the Put-in Bay station the fry are planted by pouring them over the sides of the vessel as it moves slowly along, and the superintendent of this station, from experiments he has made, believes this method preferable to the use of hose or tin conductor as just described. 756 BULLETIN OF THE BUREAU OF FISHERIES. None of the salmon stations on the Pacific coast is of sufficient capacity to hold more than lo per cent of the fry until sac absorption, and in California a large portion of the product is distributed to hatcheries operated by the Cali- fornia Fish Commission. The portion distributed by the Bureau from the Baird station, however, is held tmtil sac absorption. The method of distributing from Baird is as follows: The inflow to a trough is shut off, the drain pipe removed, and the water and fry allowed to pass into a double receptacle consisting of a perforated bucket inside a regular 5-gallon spawn bucket, the inner container being about i inch less in diameter and raised an inch from the bottom by wooden blocks. This receptacle has been filled with water, to prevent injury to the fry as they are poured in, and the surplus water escapes through the perforations and over the rim of the outside bucket, leaving the fry in the center. If the trough is some distance from the floor a box is used to elevate the buckets to proper level, and any fry remaining in the trough after the passage of the water are brushed through the opening with a broad, flat paint brush. By this method the fry can be removed from a trough in two minutes with absolutely no loss. From the bucket the fry can be easily poured into a lo-gallon can, but frequently they are carried to the river (about 100 feet from the hatchery) and planted direct from the buckets. So far as possible the plants are made during flood water and always where there is a strong current. By this means few, if any, free swimmers are caught by the ever-present trout, as their natural tendency quickly to scatter is facilitated by swift water. DISTRIBUTION OF GAMU FISHES. The Bureau does not as a rule attempt to plant the game fishes produced at its hatcheries, but consigns them to individuals, anglers' clubs, protective associations, etc., by whom they are used to stock both public and private waters. It is customary to deliver the fish free of charge to the applicants at the railroad stations nearest the point of deposit. The number of fish allotted to individual applicants is, of course, largely determined by the supply available, which depends to great extent upon the difference in methods of hatching applicable to the different species. The area and character of the water to be stocked must also be considered, of course. Moreover, the same water area that would receive a million pike perch fry would perhaps be assigned no more than 200 or 300 black bass 3 or 4 inches long, or four to eight times that many if the bass are planted as fry. The explanation is in the fact that pike perch can be propagated by the hundred million, while black bass, hatched by other methods, or collected from overflowed lands, are pro- FISH-CULTURAI. PRACTICES IN THE BUREAU OF FISHERIES. 757 duced only in comparatively small numbers. The Bureau does not attempt to assign any applicant more than a liberal brood stock of the basses or sun- fishes. With brook trout, which are distributed both as fry and fingerlings, assignments of fry are twenty-five to fifty times larger than assignments of fingerlings 3 to 4 inches long. Applicants for fish are advised by mail of the approximate date on which the fish will be shipped and later by wire of the hour on which they may be expected to arrive. The advance mail notice also contains the specific instruc- tions for the care of the fish from the time of delivery until they are planted. LIBRARY OF CONGRESS