CORNELL UNIVERSITY. THE Bostncll P» ^lotocr Cibrarg THE GIFT OF ROSWELL P. FLOWER FOR THE USE OF THE N. Y. STATE VETERINARY COLLEGE. 1897 Cornell University Library QP 34.A82 1893 Notes on physiology, 3 1924 001 040 207 Cornell University Library The original of this book is in the Cornell University Library. There are no known copyright restrictions in the United States on the use of the text. http://archive.org/details/cu31924001040207 PHYSIOLOGY LIBRARY. NOTES %. ^ ON PHYSIOLOGY HENRY ASHBY, M.D. Lond., F.R.C.P. THYSICIAN TO THE GENERAL HOSPITAL FOR SICK CHILDREN, MANCHESTER LECTURER ON DISEASES OF CHILDREN, FORMERLY LECTURER ON ANIMAL PHYSIOLOGY, OWENS COLLEGE ; DEMONSTRATOR OF PHYSIOLOGY, LIVERPOOL SCHOOL OF MEDICINE §i*ijr (Sbiiion, xltasiraiefr LONDON LONGMANS, GREEN, AND CO. AND NEW YORK : 15 EAST 16'" STREET 1893 ft Ale rights reserved PREFACE TO THE SIXTH EDITION In preparing the sixth edition, the text has been thoroughly revised and many chapters re-written. Seven new woodcuts have been added. H. A. St. John Street, Manchester : March 1893. PREFACE TO THE FIRST EDITION These Notes were originally compiled for the use of students of the Liverpool School of Medicine, when preparing for the primary examination of the College of Surgeons. They now appear in print, in the hope that they may prove useful to a wider class of students. The information they contain is founded, to a large extent, on Quain's 'Anatomy' (8th ed.), Gray's 'Anatomy,' and Foster's 'Text-Book of Physiology,' to which works the student is referred for his general reading. Fifty questions, taken for the most part from the Calendar of the College of Surgeons, are added. H. A. Manchester : Sept. 1878. CONTENTS CHAPTER I PHYSIOLOGICAL CHEMISTRY PAGE Inorganic salts — Organic crystalline bodies — Carbo- hydrates — Fats — Proteids— Albuminoids . . i- CHAPTER II PHYSIOLOGICAL HISTOLOGY Epithelium — Pigment ...... 19-28 CHAPTER III THE CONNECTIVE TISSUES Connective tissue— Retiform tissue — Adipose tissue . 28-35 CHAPTER IV CARTILAGE AND BONE Hyaline cartilage — White fibro-cartilage —Yellow elastic cartilage — Structureof bone— Development ofbone 35-48 CHAPTER V MUSCLE Striated muscle — Muscular fibre of heart — Non-striated muscular fibre — Chemistry of muscle — Physical pro- perties of muscle — Effects of muscular exercise — Stand- ing — Sitting — Walking ..... 48-64 viii Physiology CHAPTER VI SKIN PAGE The epidermis— The dermis— Sweat-glands —Hairs —The perspiration ....... 64-71 CHAPTER VII THE BLOOD Red corpuscles— Haemoglobin — White corpuscles — Liq. sanguinis — Serum— Gases of the blood — Coagulation of the blood -Quantity in the body . . . 72-86 CHAPTER VIII THE CIRCULATION The heart —A cardiac revolution —Cardiac impulse— Work done by heart— Frequency of pulsations —Endocardial pressure — Innervation of the, heart — Ganglia — Action of vagus — The arteries — Arterial pressure — The pulse — The capillaries —The veins — Innervation of the blood- vessels —Action of poisons on the circulation . 87-116 CHAPTER IX LYMPHATIC SYSTEM Modes of origin -Lymphatic vessels —Thoracic duct - Lymph —Chyle —Movements of lymph — Lymphatic glands 117-124 CHAPTER X RESPIRATION Trachea and bronchi — Infundibula— Air-cells - Mechanism of respiration— Vital capacity —Changes of the air in respiration— Dyspnoea— Nervous mechanism of respira- tion —Coughing — Effects of respiration on the circula- tion 124-143 Contents ix CHAPTER XI ANIMAL HEAT PAGE Cold- and warm-blooded animals — Gain and loss of heat to the body — Heat generated in the body— Distribution of heat— Regulation of heat ..... 143-147 CHAPTER XII FOOD Nitrogenous food — Meat — Eggs — Cheese — Destiny of nitrogenous food — Dynamic value of proteids — Fats — Destiny of fats — Amyloids — Inorganic materials — Dietetics — Diets — Effects of insufficient diet— Effects of starvation ....... 147-163 CHAPTER XIII' DIGESTION Teeth — Structure of teeth— Chemical composition — De- velopment — The tongue — The mouth — Papilla — Tonsils — Mastication — Salivary glands — Saliva — In- nervation of salivary glands — Deglutition — The oeso- phagus — Stomach — Gastric glands — Gastric juice- Chyme — Vomiting — Structure of small intestine— Bile — Pancreas — Pancreatic juice — Large intestine — De- fecation . . . . . . . . 163-200 CHAPTER XIV ABSORPTION AND NUTRITION Albuminous foods — Starches — Fats .... 200-203 CHAPTER XV THE LIVER Fissures — Lobes— Lobules — Vessels — Functions of liver — Glycogen — Secretion of bile .... 203-210 Physiology CHAPTER XVI THE KIDNEYS PAGE Structure— Malpighian bodies — Convoluted tubes— Blood- vessels — Urine — Estimation of urea — Secretion of urine— The bladder— Micturition . . . 211-228 CHAPTER XVII THE DUCTLESS GLANDS Spleen — Supra-renals — Thyroid — Thymus . 229-236 CHAPTER XVIII NERVOUS SYSTEM Nerves — Terminal end-organs — Structure of grey matter — Ganglia — Functions of nerves — Electrical pheno- mena of nerves — Classification of nerves — Nerve centres — Automatic actions — Reflex actions — Spinal cord — Functions of the cord — Medulla oblongata — Pons Varolii — Mesencephalon — Corpora quadrigemina — Crura cerebri — Cerebellum — Thalamencephalon — Corpus striatum — Cerebrum — Speech centre — Cranial nerves— Sympathetic ...... 237-306 CHAPTER XIX THE SENSES Smell — Taste — Tactile impressions — Feeling — The eye — The ear 306-333 CHAPTER XX MECHANISM OF SPEECH The larynx— Articulate sounds . .... 333-340 Contents xi CHAPTER XXI ORGANS OF GENERATION PAGE Uterus — Ovaries— Ovum — Impregnation — Chorion — Yolk- sac — Amnion — Allantois — The placenta — Foetal circula- tion — The mammary glands — Secretion of milk— The testes — The spermatozoa ..... 341-363 CHAPTER XXII THE PHASES OF LIFE Infancy— Childhood— Youth— Adult age— Old age . 363-364 APPENDIX Metric System 365 Questions on Physiology 366 Index 375 CHAPTER I PHYSIOLOGICAL CHEMISTRY The ultimate constituents of the human body com- prise some fifteen or sixteen of the elements. They are — • Oxygen Sulphur Sodium Silicon Hydrogen Phosphorus Potassium Fluorine Carbon Chlorine Magnesium Lithium Nitrogen Calcium Iron Manganese Only three of the above elements occur in the body in their free state ; viz. Oxygen, which enters through the lungs and is found in all the fluids of the body, either in solution or loosely combined ; Nitrogen, also found dissolved in the fluids of the body ; Hydrogen, which occurs as a product of decomposition in the alimentary canal. These sixteen elements are combined in various proportions, to form the compounds which exist in the tissues of the body. The simpler bodies are crystal- line in form, as chloride of sodium and urea ; the more complex, as albumin, are amorphous. The former, being crystalloids, readily pass out of the body through the excretory organs ; the latter, being colloids, are better suited to form part of its tissues. 2 Physiological Chemistry These bodies may be divided into the following classes : — I. Inorganic Compounds. II. Organic Crystalline Salts, or the Urea Group. III. Carbo-hydrates, or Sugars. IV. Hydro-carbons, or Fats and their Allies. V. Albuminous, or Proteid Compounds. VI. Albuminoid, or Gelatinous Compounds. I. The Inorganic Compounds include water, acids, bases, and salts. Water, H 2 0, forms about 70. per cent, of the whole body ; it is one of the chief constituents of the juices and tissues, and is a general solvent, by means of which various materials may be taken in as food, or excreted from the body. The various organs or liquids contain variable quantities ; thus, enamel con- tains 2 per cent. ; saliva, 99-5 per cent. Acids consist of — Hydrochloric, which exists free in the gastric juice and in combination with bases in all the tissues and fluids. Carbonic, with bases in blood, teeth, and bones. Phosphoric, in combination with bases, in the bones, teeth, corpuscles, brain, &c. Sulphuric, with bases in blood, serum, and secretions. Hydrofluoric, with bases in bones and teeth. Silicic, with bases in hair, epidermis. Bases — Sodium, in all tissues and fluids. Potassium, in the muscles, red blood corpuscles, nervous tissues, secretions. Ammonium, sparely in the gastric juice, urine, saliva. Urea 3 Calcium, in bones and teeth and fluids. Magnesium accompanies lime. II. The Organic Crystalline Bodies are very numerous ; for the most part they are the result of the disintegration of albuminous material, and nearly all contain nitrogen. The principal members of this group are urea, uric acid, xanthin, hypoxanthin, hippu- ric acid, kreatin, kreatinin, lactic acid, lecithin, neurin, cerebrin, leucin, tyrosin, and cholesterin. Urea, CH 4 N 2 or CO(NH 2 ) 2 , forms the chief- constituent of the solid portions of the urine of man and the carnivorous animals ; it is also found, but less freely, in the urine of herbivorous animals, rep- tiles, and birds. It exists in minute quantities in the blood, lymph, and liver. It is found in larger quan- tities in all the fluids of the body in advanced Bright's disease. Preparation — artificially. — By heating a mixture of potassic ferrocyanide and manganic dioxide on an iron sheet, potassic cyanate is formed, and is dissolved out with water. The potassic cyanate is treated with ammonium sulphate, ammonium cyanate and potassic sulphate being formed ; the potassic salt is removed by crystallisation, and the mother liquor, on evapora- tion to dryness, and extraction of the dried residue with alcohol, yields urea. It can also be made artificially by heating ammo- nium cyanate, a rearrangement of atoms taking place. NH 4 CNO = (NH 2 ) 2 CO Ammonium cyanate = Urea. From urine. — The urine is evaporated to a thin syrup, and its own volume of colourless nitric acid added ; nitrate of urea is formed and readily crystal- lises. The nitrate is dissolved in water, decolorised by animal charcoal and recrystallised. To obtain the urea pure, the nitrate is decomposed by barium 4 Physiological Chemistry carbonate, the barium nitrate which is formed is allowed to crystallise out, and the liquor containing urea evaporated to dryness and extracted with alcohol. Properties. — Urea crystallises from water in long thin colourless needles. If formed slowly the crystals are four-sided, and have pyramidal ends. It is a colourless substance of saline taste, soluble in water and alcohol, insoluble in ether. Urea is closely related (being isomeric) with ammonium cyanate, NH 4 CNO, and carba- mide, (NH 2 ) 2 CO. Amides are com- pounds in which hy- Fig. i.-Crystals of nitrate of urea. drOXyl HO is replaced by NH 2 . Thus, if in the molecule of carbonic acid, CO(HO) 2 , we replace one HO we get monamide or carbamic acid ; if we replace both we get carbamide or urea. CO HO Carbonic acid. CO HO Monamide. co NH 2 Carbamide, or urea. Characteristic reactions and tests. — (i) Pure nitric acid gives in a strong solution of urea a crystalline precipitate of urea nitrate. These crystals are colour- less six-sided prisms, and are sparingly soluble in alcohol (fig. i). (2) Mercuric nitrate gives a white precipitate in the absence of chlorides. (3) Nitrous and hypobromous acids decompose urea, nitrogen and carbonic acid being liberated. Uric Acid CO(NH 2 ) 2 + 3 HBrO = C0 2 + N 2 + 2 H 2 + 3 HBr Urea + Hypo- =Carbonic + Nitro- + Water + Hydro- bromous acid acid gen bromic acid. (4) Fused with caustic potass, or treated with con- centrated sulphuric acid, urea is resolved into ammonia and carbonic acid. The same change takes place in the presence of decom- posing animal matters as in stale urine, the urine becoming ammoniacal : — CO(H 2 N) 2 + 2 H 2 Urea + Aq. = (NH 4 ) 2 C0 3 . = Ammonium carbonate. Uric Acid, C 5 H 4 N 4 03, is present in small quantities in the urine of man and the carnivora, in smaller quantities in that of the herbivora. It never occurs in the free state in normal urine, but in com- bination with soda, potash, or ammonia. It is present in the spleen, liver, and also in the blood in gout ; and 'urinary calculi are often composed of it. It forms about 90 per cent, of the solid residue of the urine of snakes, and is present in large proportion in the urine of birds. In birds and reptiles uric acid is the medium by which nitrogen escapes from the system, thus taking the place of urea. Preparation.— -It is best obtained from the ex- crement of snakes, by boiling with caustic potass until the urate of ammonium of which it consists is Fig. 2. — Crystals of uric acid from urine. 6 Physiological Chemistry decomposed, the ammonia being driven off. The uric acid is precipitated in an impure state by adding hydro- chloric acid. The precipitate is re-dissolved in potass and re-precipitated by acid. From urine. — By acidulating with hydrochloric acid, and allowing to stand for twenty-four hours, reddish crystals of impure uric acid being precipitated (fig. 2). Properties. — Pure uric acid is a white crystalline powder, almost insoluble in cold water, insoluble in alcohol and ether. The crystals vary in shape, but are for the most part of a rhombic form. It is di- basic, and combines with bases to form soluble salts, as the urates of ammonium, potassium, and sodium. By oxidation, uric acid yields, in the presence of acids, alloxan and urea ; in the presence of alkalies, allantoine and carbonic acid. Uric acid can be prepared by heating urea and glycin together. Tests — Murexide test. — A small portion of uric acid is moistened with strong nitric acid, and evaporated at a gentle heat. It effervesces, and leaves a reddish coloration, which on adding ammonia becomes purple. Schifs test. — Uric acid is dissolved in a solution of sodium carbonate ; and dropped on paper moistened with silver nitrate a brown stain is formed. Hippuric Acid, C 9 H 9 N0 3 , occurs in small quan- tities in the urine of man and carnivora, but abun- dantly in the urine of herbivora ; in the latter it is the chief means of passing nitrogen out of the body. It is precipitated by iron salts. Heated in a test-tube, it is decomposed into benzoic acid and ammonic ben- zoate, which condense on the sides of the tube, and an oily substance remains behind. Most of its other Kreatinin 7 salts are soluble. It crystallises in fine needles (see fig. 3). _ Kreatin, C 4 H D N 3 02, exists in the muscles, and can be obtained from extract of meat. It occurs in colourless oblique rhombic prisms ; soluble in hot, Fig. 3.— Crystals of hippuric acid (Landois and Stirling). sparingly soluble in cold water. It has a neutral re- action, and when boiled with baryta water, splits up into urea and sarcosin. Kreatinin, C 4 H 7 N 3 0, is an alkaline body which exists in small quantities in muscle-extract and in urine. It crystallises in colourless prisms. Kreatin, on boiling with HC1, loses H 2 and forms krea- tinin. It can be separated from the urine by pre- cipitating with mercuric chloride. It unites with zinc chloride, forming crystals, which help to identify it (see fig. 4)- 8 Physiological Chemistry Xanthin, C 5 H 4 N 4 2 , exists in small quantities in urine, in the spleen, and muscles. It is insoluble in water, soluble in nitric and hydrochloric acid. When heated with nitric acid and evaporated, a yellow resi- due is left. It occurs in some calculi. Hypoxanthin, C 5 H 4 N 4 0, occurs in the tissues of the spleen and muscles, and has been noticed in the urine of leukaemia ; when oxidised it forms xanthin. Fig. 4. — Kreatinin, zinc chloride, a, in balls with radiating lines ; £, crystallised from water ; c, from alcohol (Landois and Stirling).' Lactic Acid, C 3 H 6 3 , is the acid formed during lactic fermentation, and is found in sour milk and in the alimentary canal. Sarco-lactic acid has the same composition as lactic, but differs from it in the solu- bility and crystalline form of its zinc and calcium salts. It is found in the muscles, and can be obtained from muscle-extract. Leucin g Indican, C 8 H 7 NS0 4 , is a substance derived from indol, the basis of indigo, formed in the intestine as a product of digestion ; it is present in variable quan- tities in the urine ; when treated with an equal quantity of HC1 and a drop of solution of chloride of lime, indigo blue is formed. Lecithin, C 44 H 90 NPO 9 , occurs in the brain, yolk of egg, pus, and in smaller quantities in the blood and bile. It is a white crystalline substance, soluble in hot alcohol and ether. Cerebrin and Neurin are two substances which occur in the brain, and the latter also in yolk of egg. Leucin, C 6 H 13 N0 2 , in conjunction with tyrosin, is found in many of the organs and fluids of the body, Fig. 5.— Crystals ofleucin and tyrosin (Salkowski and Leube). in the pancreas, liver, spleen, in the peptones of the alimentary canal, and in the urine in acute yellow atrophy and other diseases of the liver. These substances are formed during the decomposition of io Physiological Chemistry albuminous substances. They may be prepared by the artificial decomposition of albumin, fibrin, casein, gelatin, &c, but are most readily obtained by boiling horn-chips in dilute sulphuric acid. Leucin can also be obtained synthetically. Impure leucin appears under the microscope in the form of oily lumps clus- tering together (fig. 5) ; when pure it forms white flat crystals. It is soluble in water and alkalies, less so in alcohol. Scherer's test. — Place a small portion on platinum foil with a drop of nitric acid and evaporate gently. A colourless residue will be left, which, on the addition of liq. potassse, will become yellow and form an oily drop. Tyrosin, C 9 H u N0 3 , is generally found in con- nection with leucin, and consists of minute colour- less microscopic needles of a silky lustre (fig. 5). It is less soluble in water than leucin, and is insoluble in alcohol, but soluble in liq. potassse and dilute acids. Ifoff)?iann's test. — Add mercuric nitrate and boil ; the liquid will become rose-coloured and de- posit a red precipitate. Piria's test. — Add a few drops of concentrated sulphuric acid, warm, neutralise with chalk, filter, and add ferric chloride ; the liquid will become of a violet colour. Cholesterin, C 26 H 44 0, cannot be said to belong to the Urea group, for chemically it is an alcohol, and is the only member of the alcohols present in the system. It occurs in small quantities in the blood, bile, and nervous tissues. It is insoluble in water and cold alcohol ; soluble in ether, chloroform, and boiling alcohol. It occurs in white crystals, for the most part in rhombic plates (fig. 6). It is generally prepared from gall-stones by boiling with alcohol, filtering, and allowing to crystallise. With strong Grape Sugar 1 1 H 2 S0 4 and a trace of iodine it becomes of a violet colour, which afterwards changes to green and then red. III. Carbo-Hydrates. — The principal carbo- hydrates found in the animal body are : i. Grape sugar. 2. Maltose. 3. Milk sugar. 4. Inosit. 5. Glycogen. 6. Dextrin. 1 . Grape Sugar or Dextrose, C 6 H , 2 6 , occurs in small quantities in the blood and urine, and in larger quantities in the contents of the alimentary canal. When pure it forms four-sided prisms, but is generally seen in irregular warty lumps. It is soluble in water and alcohol. It un- dergoes decomposi- tion in the presence of certain ferments. It is precipitated by acetate of lead and ammonia. (a) Alcoholic fer- mentation takes place under the influence of yeast ; alcohol and carbonic acid are formed : — C 6 H l2 6 = 2 C 2 H 6 + 2C0 2 . (0) Lactic fermentation. — Under the influence of the bacilli found in decomposing animal matters, lactic acid is formed in the first instance, and after- wards butyric acid, carbonic acid and hydrogen. 1st stage, C 6 H 12 6 =2C 3 H 6 3 . 2nd stage, 2C 3 H 6 3 =C 4 H 8 2 + 2C0 2 + 2H 2 . The acidity of the contents of the large intestine is due to the presence of lactic acid. Fig. 6. — Crystals of cholesterin. 1 2 Physiological Chemistry Trommel's test. — Boil the solution with a few drops of solution of cupric sulphate and excess of caustic potass ; if dextrose is present an abundant reddish-yellow precipitate of cuprous oxide will fall. Moore's test. — Boil with caustic potass ; if sugar is present the liquid will become first light yellow and afterwards brown. Fermentation test. — Add a small quantity of yeast, and leave in a warm place for 24 hours ; a considerable quantity of carbonic acid will be evolved, which can be collected in a suitable apparatus. Alcohol will be present in the liquid. 2. Maltose, C^H^On + H 2 0, is the form of sugar which is mainly produced by the action of diastase such as ptyalin on starch. It possesses more rotatory power ( + 150) over polarised light than dex- trose ( + 58)- Its reducing power over cupric oxide is less than that exercised by dextrose. Maltose is converted into dextrose under the influence of some of the intestinal ferments, and by boiling with dilute acids, but not by the action of malt diastase. 3. Milk Sugar or Lactose, C 12 H 2i O n , is found in milk. It differs from dextrose in being more insoluble in -water, and not readily undergoing the alcoholic fermentation. It readily undergoes the lac- tic fermentation. It precipitates cuprous oxide from alkaline solutions in the same manner as dextrose. It is insoluble in alcohol. 4. Inosit, C 6 H 12 6 , occurs in small quantities in the spleen, liver, and brain, and appears in the urine in uraemia. It undergoes the lactic but not the alcoholic fermentation. 5. Glycogen, C 6 H, O 6 , is found in considerable quantities in the livers of well-fed animals, in smaller quantities in the white corpuscles of the blood, Dextrin 1 3 placenta, and foetal tissues. It is an amorphous, white, tasteless powder, soluble in water, insoluble in alcohol. Its aqueous solution is opalescent. Preparation. — Kill a well-fed rabbit shortly after a meal, quickly remove the liver, and after cutting it in slices, throw it into boiling water without loss of time. After boiling for a short time (to prevent the ordinary post-mortem change which glycogen under- goes into grape sugar) pound the liver, boil again and filter. The filtrate contains the glycogen, and certain albuminous substances which must be removed. The latter are precipitated with potassio-mercuric iodide in the presence of hydrochloric acid. The glycogen is then precipitated by adding alcohol. Tests. — Dilute mineral acids (except nitric) con- vert it into grape sugar. Iodine gives a red coloration, which disappears on warming and reappears on cooling. (Starch gives blue with iodine, dextrin red, which disappears on warm- ing, and does not reappear on cooling.) 6. Dextrin, C 6 H 10 O 5 . — Starch is converted into dextrin by the action of ferments, the dextrin formed being in turn converted into maltose if the action of the ferment is continuous. Dextrin is found in the alimentary canal and also in the blood. It becomes of a red colour on addition of iodine ; the colour disappears on warming, and does not, as in the case of glycogen, reappear on cooling. IV. Hydro-Carbons, or Fats.— The principal fats present in the animal body are : — •i Stearine ,£ h r\\ f O ( ( -18 J:1 35 < J)3 J Palmitin^ 3i0)3 }0 3 01ein (CAO)J ' 1 4 Physiological Chemistry These neutral fats, when submitted to the action of superheated steam, or heated with lead oxide, com- bine with water, and form glycerine and a fatty acid. Palmitin Glycerine Palmitic acid C3H.5 ) n H) n _C 3 H 6 l r . C 16 H 31 o (C 16 H 31 0)3f 03 + 3 Hl - H 3 I° 3+3 H U - Stearin is best obtained from beef or mutton suet. It is the hardest of the fats, and crystallises in white shining plates. It has the highest melting- point (60° C.) Palmitin is best prepared from palm oil ; it crystallises in needles and has a lower melting-point than stearin (40 C.) Olein is prepared from olive oil, and is fluid at ordinary temperatures. Glycerine, C,^* } 0,, or C 3 H 5 (OH) 3 , is a syrupy fluid with sweet taste and a neutral reaction ; it is soluble in water and alcohol, but not in ether. It dissolves many metallic oxides, and on heating decomposes, acrolein being formed. V. The Albuminous Bodies or Proteids occur ,in almost all the tissues and fluids of the body. They ^derive their name from the white of egg, which is taken as a type of the group. They will not crystal- lise, and are obtained pure with difficulty. They are insoluble in alcohol and ether, soluble in strong acids and alkalies, undergoing decomposition in the process. They are not formed in the animal body, but enter the body in the form of food derived from the vege- table kingdom. Urea is the chief product of their oxidationjwithin the body, carrying away all their N ; C0 2 and H 2 are also formed. They have the following average percentage composition : Albumin 15 O H C N S 2 1 per cent. 7-5 54 16 1 All proteids in solution give the following reac- tions : — 1. Xantlwprotein Reaction. — Heat with strong nitric acid, cool, and add ammonia. An orange colour is produced. 2. Milton's Reaction. — Add some Millon's reagent (Hg(N0 3 ) 2 + HgN0 3 ) and heat ; the fluid will become red, and if sufficient albumen is pre- sent, a precipitate will fall. 3. Biuret Reaction. — Add some liq. potassae and a drop or two of solution of cupric sulphate ; heat : a violet colour is produced. The proteids include several groups : — 1. Albumins. 4. Fibrin. 2. Derived albumins. 5. Coagulated proteids. 3. Globulins. 6. Albumoses. 7. Peptones. 1. Albumins. — The albumins are soluble in water, insoluble in alcohol and ether. They are coagulated at a temperature of 70° C. If dried at a lower temperature, they form a tasteless yellow mass. The albumins are precipitated in the following ways : — (a) By boiling and acidulating with nitric acid. \p) By concentrated nitric acid in the cold. (c) By the addition of acetic acid and potassic ferrocyanide. (d) Boiling with acetic acid and strong solution of sodium sulphate. „.7^t' 1 6 Physiological Chemistry The two different forms of albumin are serum- albumin and egg-albumin. They differ in that — (a) Egg-albumin is coagulated by ether, serum- albumin is not. (b) Coagulated serum-albumin is soluble in strong nitric acid, egg-albumin is not. (c) Serum-albumin injected beneath the skin does not appear in the urine, egg-albumin does. 2. Derived Albumins.— (a) Alkali Albumin. — If albumin in solution is treated with dilute caustic potash and gently warmed, some of its properties undergo change. The alkaline solution will no longer be precipitated by boiling. It is precipitated on neutralisation with acids, and is soluble in excess of the acid. It is not precipitated on neutralisation in presence of the alkaline phosphates. (6) Casein. — This substance closely resembles alkali albuminate, but differs from it in containing sulphur. It can readily be prepared from milk by saturating with magnesium sulphate, or by acidifying and gently warming ; it is- precipitated when milk comes in contact with the walls of the stomach, (c) Acid Albumin. — If albumin in solution is treated with HC1 or other acids, it under- goes a change in its properties. It is no longer coagulated by heat. It is precipitated on neutralisa- tion with an alkali, and is redissolved by excess ; its precipitation is not prevented by alkaline phosphates. It is precipitated on boiling with lime-water. If muscle be dissolved in dilute HC1, a body termed syntonin, closely resembling, if not identical with, acid albumin, is formed. 3. Globulins. — These bodies differ from the albumins in being insoluble in water, precipitated by C0 2 , or on saturating their solutions with NaCl. They are converted into acid albumen by HC1. They The Proteids 17 are soluble in dilute solutions of NaCl, the solution being precipitated by heat. ' They include (a) globulin, (b) paraglobulin, (c) fibrinogen, (d) myosin, (e) vitellin. (a) Globulin exists in the crystalline lens, and closely resembles paraglobulin in its properties, but differs from it in not assisting to form fibrin, (b) Paraglobulin occurs in blood and serum, and in -smaller quantities in some of the tissues. It gives rise to fibrin when mixed with any fluid, as hydrocele fluid, containing fibrinogen, (c) Fibrinogen exists in blood, peri- cardial, pleural, and hydrocele fluids. It closely resembles paraglobulin, but when thrown down by C0 2 it is less flocculent and more viscous, (d) Myosin is present in dead muscle. It is not so soluble as fibrinogen. It is converted into syntonin by dissolving in HC1. (e) Vitellin exists in yolk of egg ; it is soluble in dilute NaCl solutions, but differs from other members of the group in not being precipi- tated by saturating with NaCl. 4. Fibrin is obtained by whipping freshly-drawn blood. It forms tough, white strings, which are insoluble in water and dilute NaCl solution ; is con- verted into syntonin by digestion with HC1. 5. Coagulated Proteids. — When solutions of the albumens or globulins are heated to 70 C, they are coagulated. In this condition they are insoluble in water and saline solutions, but soluble in strong acids or alkalies. They are dissolved during gastric and pancreatic digestion, peptones being formed. 6. Albumoses. — When proteids are acted on by ferments (such as pepsin or trypsin) in the presence of an acid, they are dissolved and certain bodies termed albumoses and peptones are formed. The albumoses are the first products, and later pep- tones are formed. Three albumoses have been de- scribed, froto-, hetero-, and deutero-albutnose. They are C 1 8 Physiological Chemistry distinguished from the peptones by being precipitated from their solutions by saturating with ammonium sulphate. Some of the albumoses are virulent poisons if injected into the blood. 7. Peptones. — The peptones are soluble in water, they readily diffuse through animal membranes, are not precipitated by boiling, acids, or by potass-fero- cyanide and acetic acid ; thus differing from other proteids. They are precipitated by tannin, iodine, and acetate of lead. A trace of copper sulphate with excess of caustic soda produces a rose colour (pink biuret reaction). Summary of the Distinguishing Tests of the principal Proteids ( Waller) /"egg-albumin . . . ether ppt. CCi boiHn| SOn -^ serum-albumin . . . no ether ppt. No MgSO, ppt. \ globulins .... MgSO, ppt. .acid and alkali albumins . ppt. on neutralisation No coagulation J ^bumoses .... HNO^ppt. in cold, soluble on 01 ing I pe p tones .... p; n k biuret reaction of fil- 1 trate after saturation with * Am 2 SO, VI. The Albuminoids or Gelatinous Bodies. These substances, which occur as the principal con- stituents of many tissues, resemble the albuminous bodies in their composition, but differ from them in many of their reactions. They include — • 1. Mucin. 3. Chondrin. 2. Gelatin. 4. Elastin. 1. Mucin is found in fcetal connective tissue and in tendons. It occurs also in the mucous secretions, saliva, bile, gastric juice, &c, giving them their ropy consistence. It is not coagulated by boiling. It is Histology 1 9 precipitated by acetic acid. It gives the proteid action with Millon's reagent and nitric acid, but not with sulphate of copper and liq. potass. 2. Gelatin. — Bones, connective tissues, tendons yield gelatin on boiling. When dry it is a colourless, transparent body ; it swells up in cold and dissolves in hot water ; the solution, on cooling, forms a jelly. It is precipitated by tannic acid and mercuric chloride, not by acetic acid. It does not yield the proteid reactions with nitric acid, Millon's reagent, or copper sulphate. 3. Chondrin forms the bulk of the matrix of cartilage, and can be prepared by boiling cartilaginous substances in water, the solutions forming a jelly on cooling. It is precipitated by acetic acid and lead acetate. 4. Elastin. — The yellow elastic fibres present in the lig. subflava, and other parts of the body, consist of elastin. It does not dissolve in boiling water, but is soluble in boiling caustic potass. CHAPTER II HISTOLOGY The animal tissues, when examined by the aid of the higher powers of the microscope, are seen to consist of certain (1) structural elements, embedded in or sur- rounded by (2) an intercellular substance or matrix. The structural elements are either cellular in form, or consist of various forms of fibres which have originally been derived from cells. The intercellular substance consists of a finely granular or homogeneous substance in which the cells are embedded, or it may be c 2 20 Histology present only in small quantities, acting as a sort of cement to connect the cells together. When in small quantities, as between epithelial cells, it is invisible unless stained by silver nitrate. THE ANIMAL CELL The cells found in the animal body vary from ^ to -g-^ inch in diameter, and consist of two parts, (i) The protoplasm, or chief substance of the cell. (2) The nucleus, a small dark body situated near the centre of the cell, and which takes up colouring matters more readily than the protoplasm. In some cells an external membrane may be distinguished, but it is not universally present. I. Protoplasm. — By means of the higher powers of the microscope, and aided by chemical reagents, it has been demonstrated that the protoplasm — in some cells at least — is not homogeneous, but consists of a fine network of fibres resembling a sponge, and has hence received the name of the spongioplasm, or reticulum, and a hyaline substance occupying the meshes known as the hyaloplasm (enchylemd). In some cells the outer layer of protoplasm becomes hardened'so as to form a sort of enclosing membrane or capsule. The protoplasm in some of the cells is so soft that various minute bodies, such as oil globuleSj starch granules, and water globules, may be found embedded in its substance. These matters may be taken up by the cells, as in the case of the epithelial cells lining the villi of the intestines, or they may be formed in the protoplasm of the cells, as in the cells which line the acini of the mammary gland. The chief proteid present in protoplasm is plastin, a muco-globulin containing phosphorus ; lime is also present, Protoplasm 2 1 Protoplasm in an active state is able to effect im- portant changes in the blood or lymph with which it comes in contact. Thus all forms of protoplasm (1) absorb O from the lymph and give up C0 2 , heat and other forms of energy being produced. (2) The protoplasm of some cells, as, for instance, those of the mammary gland form milk, and those lining the peptic glands, pepsine. (3) Under some circumstances protoplasm is able to select certain substances from the blood ; thus, the epithelium of the kidney selects urea. The term metabolism is applied to all the changes which go on in protoplasm. Under some circumstances protoplasm is capable of certain movements ; these are best seen in the white blood corpuscle. If a white blood corpuscle is ob- served under suitable conditions, it will be seen that a portion of the protoplasm is protruded, so that the corpuscle is no longer of a rounded shape, but has become more or less elongated ; the protruded portion may be withdrawn and other processes protruded. These movements, which resemble those of an inde- pendent animal, are termed amoeboid. By means of these movements the corpuscle is able to change its position, and to find its way through the walls of the minute blood-vessels and capillaries, and wander in the surrounding tissues. These movements also enable the corpuscles to take minute particles into their interior, such as minute granules of colouring matter. These amoeboid movements are dependent upon a supply of oxygen, as the movements cease after awhile if oxygen is withdrawn ; in warm-blooded animals the movements cease if the temperature falls to 10° C. ; a few degrees of temperature above that of the normal temperature of the body increases the activity of the movements, while a still higher tem- perature arrests them. 22 Histology II. Nucleus. — The nucleus is usually a small rounded body situated near the centre of the cell. In some cases, as in some nerve cells, the nucleus occu- pies nearly the whole of the cell. In typical cases the nucleus is bounded by a wall or capsule. Examined by a high power the nucleus is seen to consist of (i) a network of fibres connected with the nucleus wall, termed the chromoplasm or nuchoplas7ii ; (2) a homo- geneous substance occupying the interstices of the chromoplasm, termed the nuclear matrix. The chromo- plasm differs from protoplasm in that it is more readily stained by logwood, carmine, and gentian violet. One or more small rounded bodies, the nucleoli, may be present in the nucleus. During life new cells are constantly being formed to take the place of those which are worn out. New cells are formed by the division of existing cells. A cell divides either by what is called ' direct ' or ' in- direct ' division. In the former case, which appears to take place rarely, there is a simple fission of the nucleus followed by a division of the cell. In ' in- direct ' division (Karyokinesis) the process of fission is preceded by some peculiar changes in the chromo- plasm of the nucleus. These changes, which are very complicated, have been observed in the cells of living animals, the time of complete fission lasting from half an hour to three hours. EPITHELIUM The various free surfaces of the body— as, for ex- ample, the external surface of the skin, the mucous membranes, the internal membrane of the arteries, and the serous sacs— are lined by cells of different characters, which form the epithelium or endothelium. The latter term is applied by some to the flattened Epithelium 23 cells which line the serous sacs, blood-vessels, and lymphatics. The epithelial cells differ very considerably in shape and size, but they agree in possessing nuclei and finely granular cell contents. Epithelial cells are connected together by a small quantity of a homo- geneous albuminous substance, termed the intercellular cement ; it is stained of a dark brown colour by silver nitrate. Blood-vessels do not penetrate between the cells, the latter being nourished by the exuded plasma. On the other hand, numerous nerve fibres may be distinguished between the epithelial cells. Epithelium may be divided into the following varieties : — 1. Tesselated or pavement epithelium 2. Stratified epithelium 3. Columnar ,, 4. Transitional ,, 5. Glandular „ 6. Ciliated „ 1. Tesselated or Pavement Epithelium.— A single layer of epithelium is found lining the pleura, pericardium, peritoneum, arachnoid, arteries, veins, capillaries, lymphatic vessels, acini of the lungs, anterior and posterior aqueous chambers of the eye, and looped tubes of Henle in the kidney. The cells consist of a thin plate with an oval nucleus, but differ considerably in shape; those lining the serous sacs being polyhedral or nearly circular (fig. 7) ; the cells lining the arteries and capillaries being elongated, and the lymphatics having epithelium, with an irregular or wavy border. The outline of these cells is readily shown by staining their intercellular cement with silver nitrate. 2. Stratified Epithelium.— In this variety there are several layers of cells ; it covers surfaces which 24 Histology ire especially liable to friction. It covers the true ;kin, forming the epidermis (fig. 8) ; it forms the superficial layer of the mucous membrane of the ;avity of the mouth, tongue, oesophagus, conjunctiva, ind vocal cords, vagina, external aperture and fossa lavicularis of the urethra. The deeper cells of the iermis are more or less round, though the deepest are Fig. 7.— Pavement epithelium Fig. 8.— Skin of negro, vertical section from a serous membrane ; (Quain's Anatomy). _ a, a, papilla? ; magnified 410 diameters £, layer containing pigment ; c. Mal- (Quain's Anatomy), a, cell- pighian layer or rete mucosum ; d, horny body ; b, nucleus ; c, nucleoli. layer of flattened cells. columnar, and form the rete mucosum ; the superficial cells are flattened, overlap at their edges, and form the horny layer or stratum corneum, which covers the soles of the feet and palms of the hand. The deeper cells are not closely applied to one another at their edges, but are separated from one another b"y minute spikes or prickles, the spaces between the spikes Epithelium 2 5 forming channels. When the cells are isolated they appear to be surrounded by the spikes, and are called '■prickle-cells' 3. Columnar (fig. 9). — This variety consists of cylindrical or club- shaped nucleated cells, the thick ends being towards the free surface. Their sides are often more or less flattened from mutual pressure, or more or less irregular from the pre- sence of lymphoid cells situated between adjoining epithelial cells. The protoplasm of the cell is granu- lar from the presence of minute vacuoles ; it may also contain mucin and fatty globules. At times the outer ends of the cells are distended by mucus, forming the so-called 'goblet' cells (fig. 10). There is always a nucleus containing a fine network. The free border of the cell is more refractile than the rest (G. 9. — Columnar cells from the rabbit's intestine (Quain's Anatomy). **, an ir- regular projection ; «, nucleus with its network ; str, the fine striated border ; a fat-globule is visible in the left-hand cell. Fig. 10— Goblet cells, highly magnified (Quain s Anatomy). The right- hand cell shows distinctly the intra-nuclear network, which radiates in the lower part of the cell, and also into the mucus-containing part. of the protoplasm of the cell, and is finely striated. Columnar cells are found lining the alimentary canal from the oesophageal end of the stomach to the anus, 26 Histology lining the ducts of glands and the olfactory region of the nose. 4. Transitional. — This variety consists of flat- tened cells on the surface, a middle layer of pear- shaped cells, their rounded ends fitting into the under surface of the flattened cells, and an inferior layer of rounded or pyriform cells fitting between the thin ends of the middle layer. The bladder, ureters, pelvis of kidney are lined by transitional epithelium, and also the larynx and pharynx, where the columnar and flat- tened cells come in contact. 5. Glandular. — The acini of the various glands of the body, as the convoluted tubes of the kidney, the salivary and peptic glands, are lined by spheroidal or cubical cells. These cells are nucleated ; their protoplasm performs the important work of separating or elaborating from the blood the materials which form the secretion of the gland. 6. Ciliated. — In some parts of the body the epi- thelial' cells are provided with minute rods which are constantly in motion, and serve to propel mucus or any minute particles in contact with them towards the orifice of the chamber or tubes whose walls they line. They vibrate at the rate of about 700 per minute. These minute rods are probably prolongations of the intra-cellular network, and their movements are independent of any nervous mechanism. Chloro- form vapour and carbonic acid gas arrest their move- ments. Weak acids or alkalies and moderate electric currents stimulate them. Ciliated epithelium is for the most part columnar in shape (fig. n). They are present in man — (a) Lining the mucous membrane of the air- passages. Commencing near the nostrils, they line the nasal cavity (except the olfactory region), the antrum, ethmoidal and frontal sinuses, the nasal and lachrymal ducts, the upper part of the pharynx, the Pigment 2 7 Eustachian tube, tympanic cavity, larynx, except the vocal cords, trachea, and bronchi till they enter the infundibula of the lungs. {b) Lining the mucous membrane of the uterus, commencing at the middle of the cervix and con- tinuing along the Fallopian tubes to their fimbriated extremities. (c) Lining the vasa efferentia, coni vasculosi, and upper part of the globus major of the testis. (d) Lining the lateral ven- tricles of the brain and central canal of the spinal cord in the child. Many of the animalcula and algae, as the paramecia, rotifera, vorticella, volvox, are provided with cilia as a means of locomotion, or for producing currents in the water, so as to carry their prey within their reach. Cilia are also found in the gills of the oyster and salt-water mussel, and doubtless serve to bring a fresh supply of oxygenated water in contact with the capillaries of their gills. In man, they probably prevent the accumulation of mucus or foreign particles on the surfaces they line, and possibly in the testicle help forward the immature spermatozoa. They are most readily obtained for the microscope by snipping a small piece from the gills of the mussel, and covering with thin glass ; they will continue to work for hours if evaporation be prevented. Fie. 11.— Columnar ciliated epithelium from nasal mucous membrane x 300 (Quain's Anatomy). PIGMENT Pigment is met with in various parts of the body, for the most part in epithelium and connective tissue cells. It is met with in epithelium cells in the external layer of the retina and posterior surface of the iris ; 28 The Connective Tissues in the deep layers of the cuticle in the dark races (fig. 8, b), the membranous labyrinth of the ear, and olfactory region. In connective tissue cells which are ir- regularly branched, it is found in the outer layer of the choroid coat, in the iris and pia mater. It also occurs in some nerve-cells. The pigment itself consists of minute brown particles, which when they escape from the cells exhibit the ' Brownian ' movements. Pigment occurs in some pathological states, as in the rete mucosum in Addison's disease and in melanotic tumours. The pigment of the choroid is evidently of use in absorbing any redundant light which enters the eye. Chemically it is characterised by the large percentage (nearly 60 per cent.) of carbon which it contains. CHAPTER III THE CONNECTIVE TISSUES Connective or Areolar Tissue is present almost universally throughout the body, serving to connect the various organs with one another, as well as to bind together the parts of which an organ consists. The muscles are surrounded by a connective tissue sheath, which penetrates into their substance, binding to- gether the fasciculi and fibres. The same tissue is present beneath the skin and mucous membranes, and forms a sheath for the arteries, veins, and nerves. It is plentifully supplied with blood-vessels and lymph- atics, and many nerves pass through its substance. Microscopically, four different elements may be seen — 1. Connective tissue cells or corpuscles. 2. White fibrous tissue. 3. Yellow elastic tissue. 4. Ground substance. Connective Tissue Cells 29 1. Connective Tissue Cells (fig. 12).— On examining the connective tissue of young animals, various cells will be seen with fine granular contents and nuclei lying in spaces in the ground substance. Some are branched, others flattened or rounded. Three kinds of cells may be distinguished, these are \h& flattened or lamellar, the granular, and vacuolated or plasma cells. The flattened cells, as their name implies, are more or less flat in shape, resembling epithelium. They Fig. 12. — Connective tissue from a young guinea-pig (Quain's Anatomy). c, flattened cell ; d, branched corpuscle ; g t granular corpuscle ; /, leu- cocyte. are often branched (fig. 12, d), their branches joining together to form a kind of network, as in the cornea. The cells in the fibrous tissue of tendons are square or oblong, and form continuous rows, as seen when the tendon is viewed longitudinally ; when viewed in transverse section they appear irregularly branched, sending their branches between the bundles of the tendon. The granular cells (fig. 12, g) are mostly rounded in form, and are coarsely granular. The 30 The Connective Tissues plasma cells are mostly elongated in shape, and are distinguished from the others by their containing vacuoles in their protoplasm. 2. White Fibrous Tissue.— When areolar or fibrous tissue is examined with a high power, it will be seen that it is principally composed of fine, wavy, parallel fibres ; these are united in bundles by a very small amount of the ground substance (fig. 13). Fig. 13. — White fibrous tissue x 400 (Quain's Anatomy). Acetic acid causes the fibres to swell up and become indistinct. On boiling they yield gelatine. 3. Yellow Elastic Tissue forms a variable proportion of connective tissue, being especially abundant beneath the skin, mucous and serous mem- branes. Microscopically, it consists of yellow, elastic, curling, branching fibres, of a larger size than the fibres of white fibrous tissue. It is unchanged by Distribtition of Connective Tissue 3 1 acetic acid and the weaker alkalies. Chemically it yields elastin (fig. 14). 4. Ground Substance. — The intercellular ma- terial present in connective tissue consists of a homo- geneous material, surrounding the cells and fibres, cementing them together. It is stained by silver ni- trate like the intercellular cement of epithelium. Distribution of White Fibrous Tis- sue. — Those connecting tissues of the body which require to be inelastic, tough, unyielding, are formed of pure white fibrous tissue without ad- mixture of yellow. Such are the tendons, fasciae, aponeuroses, most liga- ments, the periosteum, the dura mater, pericar- dium, &c. They are white in colour, and will not readily stretch. Besides the ordinary wavy fibres, they contain connective tissue corpuscles. Distribution of Yellow Elastic Tissue.— In some parts of the body an elastic material is required to connect bones together or to form the walls of blood- vessels. Yellow elastic tissue enters largely into the following structures : — 1. Ligamenta subflava of the vertebras. 2. The stylo-hyoid, thyro-hyoid, crico-thyroid ligaments, the vocal cords, and calcaneo- scaphoid ligament. 3. The middle coat of the larger arteries and veins Fig. 14. — Elastic fibres from the lig. subflava x 200 (Quain's Anatomy). 32 The Connective Tissues 4. It is present beneath the mucous membrane of the trachea, and forms the walls of the infundibula. 5. The capsule and trabecule of the spleen, lymphatic glands, and erectile tissues. 6. Forming the ligamentum nuchse of horse and ox. RETIFORM TISSUE Retiform or Adenoid Tissue consists of a delicate network formed by connective tissue cor- puscles joining their branches together. In some parts the corpuscles and their nuclei are very apparent, Fig. 15.— Thin section from the cortical part of a lymphatic gland (Quain's Anatomy), a, b, network uf fine trabecular ; c, lymph corpuscles still remaining in the meshes. whilst elsewhere but little can be seen of nuclei at the intersections of the fibres. Retiform tissue forms the stroma or framework of lymphoid tissue. In lymphoid tissue, the spaces in the network are occupied by leucocytes. It is found in lymphatic glands, solitary glands of the intestine, tonsils, spleen, &c. Adipose Tissue 33 ADIPOSE TISSUE Adipose Tissue is present in many parts of the body. It forms a layer beneath the skin, in the sub- cutaneous connective tissues, except beneath the skin of the eyelids and penis ; it forms a layer of con- siderable thickness covering the buttocks, thighs, and abdomen, in well-nourished subjects. In the internal organs it is collected around the kidneys, heart, in the joints, and folds of the omentum, but it is absent from the cranium and lungs. Structure. — Fat, to the naked eye, has a coarse or finely granular appearance from the presence of large or small lobes ; these are again made up of lobules ; each lobule has its afferent arteriole, meshwork of capillaries, efferent vein, and fat cells. The tubes and lobules are bound together by areolar tissue. Fat Cells. — When examined microscopically .the vesicles or cells are, in well-nourished bodies, round or oval in shape, 3^ to -^-n mcn in diameter (fig. 16). They are derived from ordinary connective tissue cells, and consist of a delicate envelope, m, the remains of the original protoplasm of the cell, which includes a nucleus more or less flattened, n, and fat globule distending the cell, fg. After death a bunch of crystals may be seen within the cell, cr. Under favourable con- ditions of nutrition, connective tissue cells may be seen to contain oil globules in their protoplasm, others in which, the globules are fused together and are com- mencing to push the nucleus to one side, distending the cell and converting it into a fat vesicle. In starvation the reverse takes place, the oil globules are absorbed, serous fluid takes their place, and finally these may disappear and a small branched connective tissue cell be left. The contents of the vesicles in the human body consist of olein, palmitin, and stearin. 34 The Connective Tissues Uses.— i. Adipose tissue serves as a convenient packing material, which fits in between the tissues and organs, and from its fatty nature it serves to diminish friction. For example, the subcutaneous fat covering the buttock forms a soft pad, and allows the skin to work smoothly over subjacent structures. 2. Adipose tissue is an excellent non-conductor, and "serves to retain the heat of the body. Fin. 16.— Fat cells, highly magnified (Quain's Anatomy), fg, fat globule distending a cell ; «, nucleus ; nl, membranous envelope ; cr, bunch of crystals ; c, capillary ; v, vein ; c t, connective tissue cell. 3. Adipose tissue serves to store up for future use a substance rich in carbon and hydrogen. The destiny of fat is eventually to be converted into C0 2 and H 2 0, its oxidation serving to maintain the heat of the body and give rise to muscular energy. Hy- Cartilage 35 bernating animals fatten during the autumn on starchy foods, the stored fat serving to maintain them during their winter sleep. CHAPTER IV CARTILAGE AND BONE Cartilage is a bluish or yellowish -white semi-trans- lucent elastic substance, without vessels or nerves, and surrounded by a fibrous membrane, the peri- chondrium. This membrane is richly supplied with blood-vessels, lymphatics, and nerves. It is absent on the articular surfaces. Cartilage, on boiling for some hours, yields an albuminoid called chondrin, which, like gelatine, sets into a jelly on cooling, but differs from gelatine in being thrown down by tannic acid Cartilage may be divided into — (Temporary 1. Hyaline . . \ Costal (Articular „., ,.., (White 2. Fibro-cartilage . | Ye]low 1. Hyaline Cartilage (fig. 17) is present in many parts of the body. In the foetus it forms a firm, elastic material for the skeleton, prior to the deposition of lime salts and consolidation of the bones. In the adult it supplies an elastic material as . the costal cartilages, to assist in forming the walls of the chest, its elasticity aiding in an important manner the expiratory act. It caps the ends of bones at the joints, and helps to diminish friction and lessen shock. It forms in large measure the walls of the trachea and bronchi, serving to maintain their rigidity D 2 36 Cartilage and Bone and prevent collapse. It also forms the septum and lateral cartilages of the nose, the thyroid and cricoid cartilages in the larynx. Structure. — The matrix or ground substance is finely granular and transparent, and, like the matrix m&L — 9 m '<&.: , : «'; Fig k* w e (Quain's Anatomy), a, group of two cells /», group of four ; //, protoplasm of cell ; g t fatty granules ; , nucle of connective tissue, is stained brown by silver nitrate. By long maceration the matrix can be broken up into fine fibres. The cells occupy special cavities in the matrix, called the cartilage lacuna;. Each cavity is lined by Cartilage 37 a membrane, the capsule, which in growing cartilage is thickened by the addition of a layer of the hyaline matrix, distinct from the rest. The cells consist of round, oval, or elongated little masses of protoplasm with one or two nuclei : under a high power fine fibres and minute granules can be distinguished in the protoplasm. The nuclei are finely granular in appear- ance, but under a high power this granular appearance is seen to be caused by a fine network of chromoplasm. Each lacuna generally contains one cell, but it may contain 2-8 cells ; in the latter case, cell-division is proceeding more rapidly than the formation of the ground substance. Hyaline cartilage is modified in different situa- tions : — (a) Temporary. — Cartilage forms a support for the fcetus, and a bed for the deposition of the lime salts. The cells are small, for the most part angular, provided with tails, and uniformly scattered through the matrix, except where ossification is proceeding, when they arrange themselves in columns. The matrix is very finely granular. (b) Costal. — The cells are large and collected into groups, and contain oil globules ; near the ex- terior surface the cells are flattened and lie parallel with the surface. The matrix exhibits a tendency to the deposition of lime salts, beginning from the cir- cumference of the cells, though no true bone is formed. The matrix contains some scattered fibres. The cartilages of the nose, thyroid, cricoid, trachea, and bronchi resemble costal, though for the most part no fibres are to be seen in the matrix. (c) Articular. — In the layer near the bone the cells are arranged in columns, though irregularly dis- tributed near the surface. The matrix is not prone to calcify, like rib-cartilage. Near the articular surface the cells resemble the connective tissue cells of syno- vial membrane. 3S Cartilage and Bone FIBRO-CARTILAGE i. White fibro-cartilage 2. Yellow fibro-cartilage White Fibro-cartilage differs from hyaline in having the matrix occupied by fibres of white fibrous tissue. It is consequently tougher and less elastic. Its microscopic characters resemble white fibrous tissue rather than car- tilage, consisting of parallel wavy fibres with a few cartilage cells (fig. 1 8). It is distributed in the following manner : — i. Inter-articular fibro-car- tilagesform small pads occupy- ing a movable joint, their surfaces being free and lined by synovial membrane. They greatly assist in deadening the effects of shock. They are present in the temporo-maxil- lary, sterno-clavicular,acromio- clavicular, inferior radioulnar a; articulations, and also in the £ knee-joint. "\ 2. Circumferential, serving , bright stripe ; c, row of clots in bright stripe which are enlargements of the intercolumnar septa or sarcoplasm. These septa are represented by the longitudinal lines d. The continuity of these lines through the bright stripe is difficult to see in the fresh fibre, but is distinct after treatment with acid. The columns between the longitudinal lines are the fibrillar or sarcostyles. these lines are, in reality, the sarcoplasm or interstitial substance which unites the fibrillar together, and which appear dark (fig. 27, d) ; seen in cross section the sarco- plasm appears as a fine network, as it is seen here surrounding the fibrillar These longitudinal lines Cardiac Muscular Fibre exhibit enlargements at regular intervals, the being situated in or near the clear transverse (fig. 27, c). A fine membrane (Krause's membrane) can be demonstrated running across each light stripe. Cardiac Muscular Fibre differs from ordinary striated muscle in having very faint cross stripes and no sarcolemma ; the fibres are also branched. If the fibres are acted on by osmic acid, they are seen to consist (in mammals') of oblong nucleated cells, some being forked at their extremities, and joined end to end (fig. 28). Fig. 28.— Muscular fibre cells from heart * 4 2 5 (Quain's Anatomy). «. 'ine °f junc- tion between two cells ; b, c, branching cells. 53 latter stripe s = 54 Muscle II. Non-Striated Muscular Fibre is pale in colour, is not under voluntary control, and consists of bundles of contractile cells (fig. 29). It is found in many parts of the body — walls of stomach and in- testines, blood-vessels, trachea, oesophagus, ducts, iris, &c. The cells are elongated or spindle-shaped, with an oblong rod-shaped nucleus, and are surrounded by a very delicate homogeneous sheath. The proto- plasm of the cells exhibits faint longitudinal striation (fig. 29 a) ; the nucleus shows a fine network similar to the nucleus of epithelial and other cells. They vary in length, and are ttiVo i ncn t0 Woo i ncn in breadth. The cells are held together by a transparent semi-fluid cement substance. Chemistry of Muscle Muscle when removed from the body, or shortly after general death takes place, enters into the con- dition of rigor mortis. The chemical features of dead muscle, or muscle in a condition of rigor mortis, differ considerably from living muscle. Dead Muscle is acid in reaction, contains myosin, various albumins, sugar, extractive bodies, as kreatin, sarco-lactic acid, xanthin, hypoxanthin, inosit, salts, &c. (solids, 25 per cent.). Its acidity is due to the presence of sarco-lactic acid. Potassium salts and phosphates are especially abundant. Living Muscle is faintly alkaline, contains no myosin, but a substance or substances from which myosin is formed on coagulation of the muscle- plasma, no sarco-lactic acid, glycogen instead of sugar, various albumins and extractives, as in dead muscle. The chemical changes taking place in muscle when passing into a condition of rigor mortis consist in the formation of myosin and sarco-lactic acid, change of Physical Properties of Muscle 5 5 glycogen into sugar ; carbonic acid is set free. Healthy living muscle even while at rest absorbs O and gives out C0 2 . The exchange of gases is greatly augmented during contraction. Muscle Plasma can be prepared from the muscles of cold-blooded animals by pounding the minced muscle with a 1 per cent, solution of salt, kept at a temperature of 0° C, The filtered fluid {muscle plasma) is fluid at 0° C, but clots if the temperature is raised. Muscle plasma is faintly alkaline, but becomes acid on coagulation, due to the formation of sarco-lactic acid. The body which separates from the muscle plasma on coagulation is myosin, the fluid which remains is muscle serum. Myosin may be prepared by allowing muscle plasma to fall, drop by drop, into water ; little balls of myosin are formed. Physical Properties of Muscular Tissue The most important properties of muscles are extensibility, elasticity, contractility. Extensibility. — Living muscle is extensile, i.e. capable of being extended or stretched ; this is necessary, inasmuch as when one set of opposing muscles as the extensors of the fingers contract, the opposing flexors are stretched. The muscles of the body are always in a state of extension, i.e. always slightly stretched. Dead muscle is less extensile, and its elasticity is less perfect than living muscle. Elasticity. — Muscle possesses but little elas- ticity ; a small weight will stretch it, but that little is very perfect, as it returns rapidly and perfectly to its original length. Contractility. — Both muscle and nerve in a living state are irritable, that is, they respond when a stimulus is applied. The muscle responds by con- 56 Mtiscle trading, the nerve by transmitting the stimulus to its termination. This contractility is the characteristic property of muscle. If a muscle of a recently killed frog be laid bare, and any form of stimulus applied, such as the electrodes of a battery or coil, a hot wire, a chemical substance, or a mechanical injury, it will be thrown into a state of contraction. The stimulus may be applied to muscle itself, or to a nerve in con- nection with the muscle. Rigor Mortis. — This term is applied to the stiffening which muscle undergoes at death. In the human subject rigor mortis is complete in four to six hours after death, and lasts twenty-four hours to several days. In exhaustion of muscular power prior to death, as in animals hunted to death, or in soldiers killed on the field of battle, it sets in very rapidly, and is well marked- On the other hand, in wasted bedridden patients it is ill-developed, and soon passes off. It commences in the muscles of the jaw, then affects those of the neck and trunk, next the lower and finally the upper limbs. The cause of rigor mortis is the coagulation of the myosin ; the stiffening is arrested by the injection of a 10 per cent, solution of common salt. Muscle in this condition is thicker, shorter, and firmer than living muscle ; it cannot be excited by any stimuli, it is acid in reaction, from the formation of lactic acid. It is opaque, and the electric currents have disappeared. Idio-muscular Contractions. — If in a patient suffering from phthisis or some wasting disease, a superficial muscle, as the pectoralis major, be smartly tapped, a local contraction, or wheal, is produced which slowly travels along the muscle in the form of a wave. This phenomenon is only observed in exhausted muscle or muscles in which the nutrition is impaired. Fibrillar contractions may also be seen in wasted muscles, as in progressive muscular atrophy. These Muscular Contraction 57 consist in the quivering of local muscular fibres, with- out any external stimulus being applied. Phenomena of Muscular Contraction • 1. Change in Form. — When a muscle contracts it shortens -that is, its ends come nearer together, while the muscle itself becomes thicker ; but there is no change of bulk : what it loses in length it gains in thickness ; according to Landois the volume is slightly diminished. 2. Chemical Changes during Contraction. (a) Oxygen is used up. Living muscle is constantly consuming oxygen, but more carbonic acid appears than can be accounted for by the oxygen used, (b) Carbonic acid is set free, not accompanied by a cor- responding consumption of oxygen. Probably some complex body splits up, producing these two acids. (c) Muscle is normally neutral or faintly alkaline ; when it contracts it becomes acid, the acidity being due to the formation of sarco-lactic acid. Other changes doubtless take place, of which little is known. 3. Negative Variation of Muscle Current. Whenever a muscle contracts, a change takes place in its electrical current. If a muscle when at rest, arranged so as to show its normal current, be made to contract or enter into a state of tetanus, the normal current will undergo diminution during the contrac- tion. By refined methods it has been shown that the negative variation occurs during the ' latent period ' of stimulation. 4. Production of Heat during Contraction. Venous blood coming from an active muscle is warmer than blood from muscle in a state of rest. The gastrocnemius of the frog shows an increase of about one-tenth of a degree C. for each contraction. The 58 Muscle heat developed depends to some extent on the work done. 5. Production of Sound during Contrac- tion. — A sound is emitted from a muscle during contraction. By placing the ear over a contracting muscle a deep-toned sound will be heard. 6. Phenomena of a single Muscular Con- traction. — If the sciatic nerve of a frog while still attached to the gastrocnemius, and recently removed Fig. 30. — Muscle curve obtained by pendulum myograph (Foster). A, moment when the shock is sent into the nerve ; v. the commencement, c the maximum, n the close of the contraction ; E, curve made by chronograph. from the animal, be placed upon the electrodes of an induction apparatus, and a single shock, either making or breaking, be made, the muscle gives a short, sharp contraction. If the muscle nerve pre- paration be arranged in connection with a pendulum myograph, in which the tendon of the muscle is attached to a lever recording its movements on a moving surface, the lever rising during contraction and falling during relaxation, a curve similar to fig. 30 will be produced. The time occupied in tracing the curve is marked by the vibrations of a tuning-fork, recording on the same surface ; the apparatus also marks the exact moment when the induction-shock is Mttscular Contraction 59 sent into the nerve. By this means three facts will be demonstrated : — ■ (a) There is a latent period (A to B, fig. 30), that is, a short time elapses after the entrance of the shock into the nerve before the contraction of the muscle commences. This latent period is occupied by (1) the passage of the impulse along the nerve, and (2) certain changes taking place in the muscle itself before it begins to contract. (b) There is a period of ascent or contraction (B to C). This is slow at first, then more rapid, and slower again A B B' Fig. 31. — Diagrammatic muscle curves representing electrodes placed on nerve (producing curve with dotted line) and on muscle (continuous line) (Foster). A — vj, represents whole latent period, including the time occupied by nerve impulse in travelling along the nerve, and changes in the muscle ; R - s' represents time occupied by impulse along nerve ; A — B represents latent period of muscle. before the ascent is gained. The rapidity or slowness of the ascent at the various stages depends upon the weight to be raised and the exhaustion of the muscle. (c) A period of descent or relaxation (C to D). This is more prolonged than the ascent ; it is more rapid at first than towards the end of relaxation. Exhausted muscles relax slowly. If instead of placing the electrodes on the nerve they are placed upon the muscle itself, a muscle-curve (fig. 31) will be produced ; the latent period A to B will be shorter than A to B', in consequence of the 6o Muscle time occupied by the impulse travelling down the nerve being eliminated, the latent period in this case representing preparatory changes in the muscle itself. Nerve-impulses in the frog travel at the rate of about 28 metres per second ; in man 33 metres per second. The latent period of a frog's muscle varies from -pini-th to o fryth of a second. It is shorter when the muscle is fresh and when under the influence of strychnia ; it is prolonged when the muscle is heavily weighted, and in poisoning with curare. 7. Tetanus. — If single induction shocks are made to follow each other slowly, a succession of Fig. 32.— Curve of Incomplete tetanus or clonus (Hermann^. The stimula- tions succeed each other sufficiently rapidly to conceal the influence of each stimulation. Had they been more frequent an unbroken curve would have been produced. curves are produced. But if they follow more rapidly, so that there is not time between the shocks for the muscle to relax, a condition of constant spasm is pro- duced, known as tetanus. If the tetanus be in- complete, as in fig. 32, the waves produced by successive shocks are still perceptible. This is sometimes termed clonus. In complete tetanus, as produced by the magnetic interrupter in an induction machine, these are fused into one continuous curve. In the gastrocnemius of the. frog twenty-seven shocks Muscular Exercise 6 1 per second, and rorty per second in man, are re- quired to throw the muscle into a state of complete tetanus. 8. Action of Poison on Muscle.— Curare paralyses the terminations of the motor nerves, so that stimulation of the nerve produces no contraction, while direct stimulation of the muscle produces contraction. The sensory nerves are unaffected. It produces death by paralysing the respiratory muscles. Veratria causes an excessive prolongation of the muscular contraction. 9. Fatigue. — Tired muscles are sluggish in their movements, and their contractions are weaker than normal muscles. At first the contractions increase in height and duration ; then they increase in duration, but diminish in height. Rest refreshes the muscles, the blood in the meantime bringing it nutrient materials and carrying away fatigue products. Serum- albumin, if injected into the blood, acts as a refresher, Egg-albumin, albumose, and peptones are non- nutritive, and the latter two are poisonous. 10. Properties of Non-striated Muscle.— When any stimulus is applied directly to an involuntary muscle, or to a nerve in connection with it, it contracts. The latent period is longer than in striated muscle ; the contraction takes place more slowly, but lasts longer. The force exerted, as in the uterus in parturition, or the bladder in expelling urine, may be very great. Effects of Muscular Exercise 1. On the Lungs. — Elimination of Carbon. The most important effect of muscular exercise is to increase the number of the respirations, and thereby the quantity of air passing in and out of the lungs, leading to an increased absorption of oxygen, and elimination of carbonic acid. An adult, under ordinary 62 Muscle circumstances, during inspiration draws in 480 cu. in. per minute ; if he walk four miles an hour, he draws in five times as much, or 2,400 cu. in. ; if he walk six miles an hour, he draws in seven times as much, or 3,360 cu. in. Probably the excessive absorption of oxygen and formation of carbonic acid takes place in the muscles. For the effects of exercise on carbonic acid given off during respiration, see p. 137. 2. On the Circulation. — The increased work performed by the muscles requires increased activity on the part of the heart, to keep up the supply of arterial blood. The amount of increase is usually from 10 to 30 beats during exercise. After exercise the heart's action becomes slower. Excessive exertion may lead to hypertrophy of the left ventricle. 3. On the Skin. — The minute arteries of the skin become dilated, the perspiration is increased, more water, salts, and acids pass off from the system. The amount of perspiration may be more than double the usual amount. The evaporation reduces the tem- perature of the body, which would tend to rise. There is danger of a chill after the exertion is over, the skin still remaining wet while the heat of the body has declined. 4. On the Voluntary Muscles. — The muscles grow and become firmer in substance. If, however, the exercise be excessive, after growing to a certain extent, they will waste. 5. On the Digestive System.— The appetite increases with exercise, especially for meat and fats ; this is doubtless the result of the wear and tear of the muscles and the increased elimination of carbon. Digestion is more perfectly performed, and the cir- culation through the liver and portal system quickened. 6. On the Kidneys.— The water of the urine and the salts are probably lessened in consequence of the increased perspiration. It has been shown by Muscular Movements 63 various observers, including Parkes, that during active exercise the urea in the urine is not increased, but active exercise is followed by an increased appearance of urea. It appears, therefore, that to a certain extent muscular exercise increases the elimination of urea, the urea making its appearance in the period of rest succeeding the exercise. 7- On the Temperature.— The temperature will not be increased ; the extra consumption of oxygen and the friction of the muscles tend to raise the temperature, but the evaporation from the surface of- the skin prevents much increased heat of body. Various Muscular Movements Standing. — In standing the muscles fix all the joints of the vertebras and lower extremities so as to form a rigid column ; any disturbance of equilibrium, i.e. any tendency to fall, is counteracted by muscular action. The head is fixed upon the vertebral column by the muscles of the neck ; the vertebral column is maintained in a state of rigidity by the erector spina? and other muscles ; the hip, knee, ankle, and tarsal joints are fixed by the rigid contraction of their exten- sors and flexors. Sitting. — In sitting, the body is supported on the tuber ischii, whilst the head and spine are fixed by their muscles, the muscles of the legs are relaxed and therefore at rest ; in leaning back on a support, the muscles of the back are also in part relaxed. Walking. — In walking the legs move alternately, the movements being divided into two acts : — 1. One leg, the active one, is vertical and slightly flexed at the knee and supports the weight of the body ; the passive leg is extended, being behind the other, and touching the ground with the tip of the great toe. 64 Skin 2. For the forward movement of the body, the active leg is inclined forward and the knee straight- ened out (extended), the heel being lifted off the ground, so that only the tips of the toes touch the ground ; in the meantime the passive leg leaves the ground and swings with a ' pendulum-like movement ' forward, touching the ground in front. The passive leg now becomes the active one, and act 1 is re- peated. Running. — In running the active leg as it is forcibly extended gives the impetus forward, and only one leg touches the ground at a time. CHAPTER AT SKIN Consists of — 1. Epidermis or cuticle. 2. 1 )crmis, corium or cutis vera. 3. Sweat glands, nails, hair, and sebaceous glands. 1. The Epidermis forms a protective covering over the whole surface of the body. It varies in thickness in different parts, being especially thick on the palms of the hands and soles of the feet, and wherever the skin is exposed to friction. It is moulded over the surface of the corium, covering the ridges, the depressions and the papilte. It is made up of three principal layers : (a) the horny layer, or stratum corneum, is the most superficial, and consists of layers of flattened cells, which are dry and horny, without any nucleus ; (b) the stratum lucidum, composed of several layers of nucleated cells, which are more or less indistinct, and in section appear as an almost homogeneous layer ; (c) the rete mucosa m or Malpighian The Dermis 65 layer contains, in its upper part, layers of ' prickle ' cells, and its inferior layer consists of a single stratum of columnar cells. Pigment is principally found in the lowest layer (fig. 8, b). According to Ranvier, fine varicose nerve-fibrils penetrate into the Mal- pighian layer, and end in knob-like swellings. L..M H -6 -c/ Fig. 33. — Vertical section of skin and subcutaneous tissue x 20 (Quain's Anatomy), a, horny ; b, Malpighian layer : c, corium ; e, papillae ; f, fat clusters ; £•, sweat glands ; h, ducts ; z, their openings. 2. The Dermis, or true skin, is made up of an interlacing network of connective tissue, formed of white fibrous tissue, yellow elastic tissue, corpuscles, vessels, and nerves. In some parts of the body, as F 66 Skin in the skin of the scrotum, perineum, penis, the cutis vera contains unstriated muscular fibres. There are also small muscular fibres in connection with the hair- follicles. Beneath the skin the subcutaneous tissues contain abundant adipose tissue. Numerous fine ridges are seen on the surface of the skin of the palm of the hand, and sole of the foot. The ridges are caused by rows of little elevations of the cutis vera, termed papilla. These little eminences are more or less conical, or sometimes club-shaped ; they may be Fig. 34.— Section of a sweat gland (distal end) (Quain's Anatomy). a, basement membrane ; b, lining cells ; c, lumen of tube. compound, and contain a capillary loop, nerve, and touch-corpuscle ; they project into the epidermis, and by raising it up as it were, form a ridge on the surface of the skin. They serve to increase the sensitiveness of the part, lodging a touch-corpuscle in a favourable position for receiving sensations of touch (fig. 8). 3. Sweat Glands are situated in the subcu- taneous tissue, and consist of a fine tube which forms the duct, continuous with a blind extremity which is coiled up into a ball of -^ inch in diam., and is sur- rounded by a plexus of capillaries to form the gland (fig. 33). The distal part of the gland— namely, Nails 67 some three-fourths of the coiled-up tube nearest the blind extremity — is of greater diameter than the rest, and is formed of a single layer of columnar cells, while between this and the limiting membrane is a layer of non-striated muscular cells (Klein) ; the lower fourth of the coil and also the sudoriferous canal as far as the rete mucosum consists of several layers of polyhedral cells, an external limiting membrane and also an internal limiting membrane ; the epithelium of the duct is at its mouth continuous with the epithelium of the epidermis. The largest number of sweat glands are present in the palm of the hand ; next, in the sole of the foot. Nails. — The nail consists of a root and body. The root is that part of the nail which is covered by the skin, the body the external part which ends in the free edge. The lunula is the whitish portion of the body near the root, where the skin beneath is less vascular. Structure. — The nail closely resembles the epider- mis, and is, in fact, a modification of that structure, consisting of hard and thin layers of cells on the sur- face and round moist cells beneath, corresponding to the rete mucosum. Posteriorly the nail fits into a groove which lodges its root. The part of the cutis vera to which the root is attached is called the matrix, and is provided with large papillae. The part to which the body of the nail is attached is called the nail-bed. Hairs consist of a shaft and root. The shaft of the hair is cylindrical, and covered with a layer of imbricated scales, arranged with their edges upwards. The substance of the hair consists of fibres, or elon- gated fusiform cells, in which nuclei may be dis- covered. There are also present in some hairs small air-spaces, or lacuna?. In the coarser hair of the body there is the medulla, or pith, which is occupied by small angular cells and fine fat-granules. 68 Skin The root of the hair swells out into a knob, and fits into a -recess in the skin, called a hair follicle. The follicle consists of two coats, an outer, or dermic coat, continuous with the corium, and an inner, con- tinuous with the epidermis, and called the root-sheath (figs. 35 and 36). The outer, or dermic, consists of three layers : (a) formed of connective tissue, blood- vessels and nerves ; (b) principally of corpuscles and a fibrous matrix ; (c) inner coat consists of a homogeneous membrane. The inner, or epidermic coat, comes away when the hair is pulled out, and hence is called the root-sheath. It is made up of two layers, the outer root-sheath and inner root-sheath. The outer root-sheath corresponds with the rete mucosum, and is thicker than the inner, and is composed of large rounded cells. The inner root-sheath corresponds with the horny layer. It is composed of flattened cells. The deeper cells of the inner root-sheath form what is called Huxley's layer. The bulbous root of the hair fits on to a papilla, which is very large in the tactile nasal hairs of the cat. Small bundles of involuntary muscular fibres connect Fig. 35. — Magnified view of a hair-follicle - (Quain's Anatomy). a, hair showing medulla, fibrous substance, and cuticle ; b, inner, and c t outer root-sheath ; d, dermic or external coat ; e, imbricated scales forming a cortical layer on the surface of the hair. Functions of the Skin 69 the corium with the root of the hair, so that in con- tracting they elevate the hair. The Sebaceous Glands consist of a small duct, which opens into the hair follicle, and is connected by its other end with a cluster of saccules lined with epithelium, which secrete fatty matters. Functions of the Skin. — 1. The skin every- where clothes the external surface of the body, pro- tecting the underlying parts from injury. 2. It affords support and pro- tection to the termina- tions of the sensory nerves, which render it an important sense organ. 3. It is a bad conductor of heat, and thus serves to preserve the heat of the body. 4. It is supplied with a large extent of capillary blood-vessels, and thus by its means a large sur- face of blood is exposed to the cooling influence of surrounding bodies. The dilatation or con- traction of the blood- vessels supplying the skin will help to regu- late the heat of the body. 5. The sweat- glands which it contains make it an important excretory organ. 6. It plays a subsidiary part as an organ of respiration. 7. Under exceptional circumstances, absorption takes place from its surface. Fig. 36. — Section of hair follicle (Quain's Anatomy). I, dermic coat ; a, outer layer of dermic coat ; bb, blood-vessels ; c, middle layer ; d, inner or hyaline layer : 2, epidermic coat or root-sheath ; e, outer root-sheath ; fg, inner root-sheath ; k, cuticle of root- sheath ; i, hair. 7 tne mus k deer the smallest, ^j T inch. They are oval in the camel tribe. In birds, reptiles, amphibians, and fishes the coloured corpuscles are elliptical discs, the proteus having the largest, ^^ inch by -j^ inch ; they have also a pro- minent central nucleus. The red corpuscles are soft, elastic, and while pressure changes their shape, they readily regain it. When examined shortly after being drawn from the vessels, they adhere together by their surfaces, and appear like rolls of coins. Number.— There are about 5,000,000 red cor- puscles per cubic millimetre in the body in health. This number is diminished after haemorrhages and in anaemia from whatever cause ; in one case of anaemia recorded by Gowers there were only 1,290,000 per cub. mill, present in the blood. The estimation of the number for clinical purposes is made by means of the haemacytometer of Gowers. This apparatus pro- vides the means of mixing five cub. mill, of blood with 995 cub. mill, of a solution of sodium acetate of s.g. 1025 ; a drop of this solution is then placed in a cell in the centre of a glass slip such as are used for microscopic purposes, the floor of which is divided into squares, each being ^ mill, square ; the cor- puscles settle on to these squares, and when placed under a microscope the number in each square can be counted and the total number thus calculated. Structure. — The red blood-corpuscles consist of (1) the stroma, i.e. a transparent soft framework of protoplasm ; (2) haemoglobin, a crystalline colouring matter which pervades the stroma. Effects of Reagents.— Water.— Salt Solu- tion. — According to Schafer the red blood-corpuscles consist of an envelope or membrane with fluid coloured o Effects of Reagents 75 contents. The effect of water on the corpuscles appears to favour this view, as they gradually imbibe the water and swell out (fig. 38). On the other hand, a three-quarter per cent, salt solution, or any fluid of greater density than the blood plasma, causes them to shrink by exosmosis and become crenated or horse- chestnut-shaped (fig. 38, /). Carbonic Acid.— If the horse-chestnut-shaped corpuscles be treated with carbonic acid gas, they again become smooth, , j though they do not regain their original biconcave form, but are more or less concavo-convex. 1, Tannic Acid.— If the •-£$' ^Q| horse-chestnut-shaped cor- ^* puscles are treated with F ^^ ™ H *=£££ °- 2 per Cent, tannic acid, "•, corpuscle seen edgeways, , t . , 1 l • slightly swollen ; b-c, one of the their haemoglobin Sepa-, sides bulged out; d, spherical rates itself from the stroma *°™ ; ": decolorised stroma; _ , . . _/, horse-chestnut-shaped effect of Ot the COrpUSCleS, and IS salt solution ; g, action of tannin extruded in drop-like «p°n a red corpuscle, masses. From this experiment it has been stated that the corpuscles are formed of a colourless stroma con- taining hemoglobin (fig. 38, g). Boracic Acid. — In newt's blood, treated with 2 per cent, boracic acid, the nucleus becomes of deeper colour at the expense of the disc, and a fine network of fibrils is displayed, which pervades both disc and nucleus. This fine network is occupied normally by haemoglobin, and a homogeneous inter- stitial substance. Chemical Constituents of Red Corpuscles. 1. Haemoglobin. 3. Salts. 2. Globulin. 4. Gases. 5. Water. 7 6 The Blood i. Haemoglobin contains C.H.O.N.S.Fe., and forms 90 per cent, of (dried) red corpuscles. It is soluble in water and serum, crystallising in man and many mammals in elongated rhombic prisms, octa- hedral in the guinea-pig, and hexagonal in the squirrel. It can be obtained in crystals from the guinea-pig, Fig. 39. — Blood-crystals, magnified. 1, human blood ; 2, guinea-pig ; 3, squirrel ; 4, hamsler. dog, rat, or mouse, but with difficulty from the blood of sheep, ox, or pig (fig. 39). Preparatioti. — The haemoglobin is made to leave the corpuscles by shaking with ether or by alternately freezing and thawing the blood. The blood is thus rendered translucent or ' laky ' ; one quarter of its bulk of alcohol is added, and it is placed in a tem- perature of 0° C. to crystallise. Hsemoglobin exists in the human blood in two forms, one in loose combination with oxygen — oxy- Hcemoglobin hemoglobin— and the other as reduced haemoglobin. During the circulation of the blood, the O in combina- Red. Orange Blue. Reduced Hsematin bo A a b C Eh F Fig. 40.— Spectra of haemoglobin and its compounds. tion with haemoglobin is very readily given up to the tissues. If oxy "hemoglobin be acted upon in solu- tion with a reducing agent, as a solution of ferrous 78 The Blood sulphate and tartaric acid with excess of ammonia, it is reduced and becomes of a purplish red colour. Oxy-haemoglobin gives in the spectrum two narrow dark bands in the yellow and green, reduced haemo- globin a single broad dark band intermediate in posi- tion between the two (fig. 40). Haemoglobin readily decomposes, forming haematin and globulin. Haemo- globin gives a characteristic blue colour when treated with tr. guaiaci and solution of peroxide of hydrogen. Methaemoglobin is found in old blood-stains and in bloody urine ; it gives four bands in the spec- trum (fig. 40, 5.). CO-Haemoglobin is a more stable compound than oxy-haemoglobin, and is formed in the body when CO is inhaled, carbonic oxide displacing the oxygen in the haemoglobin, the animal quickly dying. CO-haemoglobin is of a florid red colour, and gives two absorption bands in the spectrum very like- those of oxy-haemoglobin, but they are nearer the violet end of the spectrum and somewhat nearer together. These bands may be seen in the examination of the blood of persons poisoned by coke or charcoal fumes (fig. 40, 3). Haematin is a black amorphous body containing iron, and is formed when haemoglobin is decomposed ; it is insoluble in water but soluble in dilute acids or alkalies ; acid haematin gives four absorption bands in the spectrum, alkaline haematin gives one absorp- tion band, and reduced alkali-hsematin gives two (fig. 40, 6, 7). Hajmin. — Haematin forms with HC1 a com- pound called hasmin, which crystallises in minute rhombic prisms. Haemin crystals are prepared by adding a small crystal of common salt to dried blood on a slide, and an excess of acetic acid : on gently heating and allowing to cool, the crystals form. The presence of these crystals is used as a test for blood. Origin of the Red Corpuscles 79 Haematoidin. — Crystals of hasmatoidin are found where haemoglobin has decomposed, as in old clots of blood in the body. It is supposed to be identical with bilirubin. 2. Globulin or Paraglobulin. See Coagulation. 3. Salts. — These amount to 1 per cent, of the dried solids, the principal salts being those of potas- sium and phosphates. 4. Gases. — Oxygen loosely combines with the haemoglobin. Nitrogen in small quantity. The amount of carbonic acid gas in the corpuscles is un- certain ; by far the greater part exists in the serum (see p. 83). 5- Water forms 56-5 per cent, of the corpuscles. Origin of the Nucleated Red Blood-cor- puscles in the Embryo. — From cells in the vas- cular area of the mesoblast. These mesoblastic cells become branched, and their processes join together, so that an irregular network of granular corpuscles is formed. The nuclei multiply, some form corpuscles which acquire a reddish colour, others remain to form the epithelium of the capillaries. The first formed red corpuscles are nucleated cells, exhibit amceboid movements, and multiply by division. In the human embryo up to the end of the first month all the red corpuscles are nucleated. These primary nucleated cor- puscles are gradually succeeded by ordinary red cor- puscles, and before the end of intra-uterine life the nucleated ones have disappeared. Origin of the Red Blood-discs in the Adult. — 1. From the nucleated corpuscles in the red marrow of bone (Neuman's cells). Peculiar cells are seen in the marrow filling the spaces of the cancellous tissue of the ribs, flat bones, and the ends of the long bones, which are apparently intermediate forms between marrow cells and red blood-corpuscles. They closely resemble the nucleated red corpuscles 80 The Blood of the embryo. 2. From the leucocytes or white blood- corpuscles. 3. From the blood-platelets. Fate of Red Corpuscles. — Probably broken up in spleen and liver. Haemoglobin probably forms bile-pigments. White Corpuscles or Leucocytes The white corpuscles in human blood are spheroidal, finely granular masses of g-gVo inch, in diameter. In a cubic millimetre of human blood there are about 10,000 white corpuscles. Some of them are of less size, being smaller than the red cor- puscles. They have a lower specific gravity than the red. They have no cell-wall, and their substance consists of protoplasm. According to Heitzmann, their granular appearance is due to a fine intercellular network, having small dots at the intersections of the network. In the meshes of the network there is a hyaline substance. They possess one or two nuclei, which are readily brought out by acetic acid. When examined in a fresh state, especially if placed on a warm stage, they exhibit spontaneous change of shape like the amoebae, these movements being termed amoeboid. The movements consist in a protrusion of processes of protoplasm, which are retracted and other processes protruded. Both in human and newt's blood there are some colourless corpuscles which contain coarser granules than others ; these are called granular corpuscles. The white corpuscles will take up coloured foreign particles, as vermilion. They are found in various tissues of the body, as in the meshes of the retiform tissue of lymphatic glands, tonsils, solitary glands, &c. In inflammation they pass through the walls of the capillaries into the tissues. They are present in the blood in the proportion of 1 per 300 red corpuscles White Corpuscles 81 after a meal, and i per 800 during fasting ; they are much more numerous in some diseases, as in leuco- cythaemia. Composition :— 1. Several albuminous substances. 2. Lecithin and glycogen. 3. Salts, mainly potassium and phosphates. 4. Water. Origin. — Probably from the lymphoid tissues of the body, i.e. lymphatic glands, solitary glands, spleen, &c, by division of the leucocytes existing there. The thoracic duct and lymphatics are constantly pouring white cells into the blood, derived from the mesenteric and other lymphatic glands. Fate. — They are possibly converted into red cor- puscles. During inflammation they pass through the capillary walls, and are converted into pus cells ; it is also probable they are utilised in other ways than in forming pus, possibly being converted into the cell- elements of new tissues, or taking the place of worn- out cells throughout the body. They seem to play a part in the formation of fibrin ferment. Blood-platelets. — These are colourless, oval discs, found in the blood, and named blood-platelets, by Bizzozero. They are best seen in the blood of the I guinea-pig. Liq. Sanguinis is a clear yellow alkaline fluid in which the corpuscles float. It may be obtained by allowing the slowly coagulable blood of the horse to stand in a tall vessel surrounded by ice. The tem- perature of o° C. prevents coagulation, the corpuscles subside, and the clear fluid may be removed by pipette. Its Composition may be described as blood minus the blood corpuscles. Serum. — When blood has coagulated, and the G 82 The Blood clot separated, a thin yellow transparent alkaline fluid is left, of specific gravity 1028. Human serum consists of — I. Albumin 4-5 per cent 2. Paraglobulin . 3'i 3- Extractives . ■2 „ 4- Fatty matters •2 ,, 5- Salts . ■85 „ 6. Water and gases . 9i- 1. Albumin exists in combination with the so- dium as an albuminate. It is in the form of serum- albumen, differing from egg-albumen in not being coagulated by ether. On boiling the serum, the albumen coagulates ; the fluid, after being deprived of its albumen, is called serosity. 2. Paraglobulin (serum-globulin), one of the fibrin factors, is present, all the fibrinogen disappearing during coagulation. 3. Extractives include kreatin, kreatinin, urea, uric acid, and traces of grape sugar. 4. Fatty Matters in minute division, and com- bined with sodium as soaps. 5. Salts, principally sodium salts, in combination with CI and C0 2 , smaller quantities of potassium and calcium phosphates and sulphates. 6. Gases. — C0. 2 , partly free, and partly in com- bination with the sodium. Gases of the Blood In human blood it has been calculated that 100 vols, of blood contain : — o co = Arterial blood 20 vols. 39 vols. Venous blood 8-12 „ 46 „ 1-2 measured at o° C. and 760 mm. N 1-2 VOls. Gases of the Blood 83 Oxygen is present in arterial blood in the pro- portion of 20 per cent. ; in venous blood the amount necessarily varies according to whether the blood has passed through an organ in a state of activity or in a state of rest. In asphyxia oxygen may be entirely absent from the blood. Nearly the whole of the oxy- gen is in loose chemical combination with the hemo- globin of the corpuscles. The oxygen of the blood can be expelled from it by means of the mercurial air- pump ; no oxygen escapes till the pressure is reduced to 125 mm., or about -j=th of the ordinary atmospheric pressure ; the gas is then rapidly given off and the blood becomes dark. The oxygen can also be ex- pelled by passing other gases, CO, NO, N or H, through the blood. It can also be extracted by reduc- ing agents, as ammonium sulphide and Stokes's fluid (sulphate of iron, tartaric acid with excess of ammonia). Carbonic Acid.— This gas is present in arterial blood in the proportion of about 39 per cent. ; in venous in variable amount, according to the vein from which it is taken and the activity of the organ yielding it ; it averages 46-50 per cent. C0 2 is present in the liq. sanguinis in combination with sodium ; a part, the ' loose ' C0 2 , so called on account of its being readily given off in the mercurial air-pump, is in com- bination with sodium as the hydro-sodic carbonate (NaHC0 3 ) ; and part also as 'fixed' C0 2 , as it can only be expelled by an acid, being in the form of sodic carbonate, Na 2 C0 3 . When hydro-sodic car- bonate is exposed to diminished pressure in the air- pump, C0 2 is given off as follows : — 2 NaHC0 3 =C0 2 + Na 2 C0 3 + H 2 0. Carbonic acid -is also present in small quantities in the red corpuscles in loose chemical combination (Ludwig). Nitrogen exists simply dissolved in the blood. 84 The Blood Coagulation of the Blood Blood drawn from a living animal into a beaker first becomes viscid and then is converted into a jelly. This jelly is of the same bulk as the previous blood. Finally, the jelly contracts, forming the clot, and a yellow clear liquid, the serum, oozes out. In man blood becomes viscid in two or three minutes, forms a jelly in five or six minutes later, and a few minutes later still the serum begins to appear. In the horse coagulation goes on more slowly, so that the cor- puscles have time to sink before the jelly stage is reached ; so that a yellowish stratum is formed on the top, free from red, but containing white corpuscles, called the ' buffy coat.' This buffy coat appears in human blood in certain inflammatory conditions. Many circumstances favour or postpone the co- agulation of the blood. The principal are — Circumstances favouring Coagulation 1. Contact with foreign matter. 2. Moderate temperature, ioo c to 120° F. 3. Stasis of blood in the vessels, or injury to or inflammation of the lining membrane. Circumstances retarding Coagulation 1. Contact with lining membrane of the blood- vessels. 2. Cold (o° C.) indefinitely postpones. 3. Addition of neutral salts, or the caustic alkalis. Contact with foreign matter quickly determines coagulation, while contact with the endothelium of the blood-vessels exercises a restraining influence. If the jugular vein of a horse be ligatured at both ends Coagulation of the Blood 85 and cut out, the blood will remain fluid, apparently indefinitely or until decomposition sets in, but will clot on being withdrawn. Blood will remain fluid for several days in the excised heart of the turtle. Horse's blood allowed to stand surrounded by ice will remain fluid indefinitely. Blood drawn into a saturated solution of sodic phosphate will remain fluid, but will clot if diluted. The immediate cause of coagulation is the forma- tion of fibrin, of which blood yields about -2 per cent. According to A. Schmidt, fibrin is formed by the union of two albuminous bodies present in the blood — paraglobulin and fibrinogen ; a third body, of the nature of a ferment, is essential, or at any rate favours the process. The ferment is derived from the white blood-corpuscles. According to Hammarsten coagu- lation is brought about by the conversion of fibrinogen into fibrin, under the influence of fibrin-ferment. Paraglobulin playing only a minor part in that, it leads to a more abundant clot when present. Injec- tion of albumose into the blood-vessels of a dog prevents coagulation, as blood drawn shortly after fails to clot. In some diseases, such as diphtheria, albu- moses are formed in the blood and tissues, and act as virulent poisons, profoundly altering the blood, and giving rise to a non-coagulation of the blood and a tendency to haemorrhage. Fibrin. — This substance may be obtained by stirring some freshly- drawn blood with a stick or bundle of twigs. It is a white stringy body, insoluble in water or alcohol, soluble in alkalies, lactic, phos- phoric, and acetic acids. HC1 converts it into syntonin. Paraglobulin (Serum-globulin) may be ob- tained from serum by passing through it a stream of C0 2 , or saturating it with NaCl or MgS0 4 . It is thrown down as a granular white precipitate. 86 The Blood Fibrinogen may be obtained in a similar manner by passing C0 2 through hydrocele or pericardial fluid, or saturating with NaCl. These fluids, however, are not constant in their compositions, and sometimes they contain no fibrinogen. The Ferment is obtained by adding defibrinated blood to twenty times its bulk of alcohol ; a precipitate of albuminous bodies with the ferment is thrown down. Distilled water dissolves out the latter, and if added to a solution containing fibrinogen and paraglobulin, coagulation quickly ensues. It has not been isolated as a solid substance. Amount of Blood in Body. — Probably about ■yzth. of the body-weight as estimated by the hemo- globin of the blood. By calculating the amount of blood escaping from the body of a decapitated criminal, by weighing the solid residue, after the addition of the blood washed out of the blood-vessels by injection of water, Lehmann estimated the amount of blood as being i in 8. In a new-born child it is i in 19. Quantitative Composition of Human Blood as a whole : — Water . . 790 parts Fibrin . 2 „ Haemoglobin • 140 » Albumen, &c. • 60 „ Salts . ■ 8 „ The Circulation 87 CHAPTER VIII THE CIRCULATION The circulation is carried ori by means of the — 1. Heart, beating about seventy per minute, alternately receiving blood from the venous system, and discharging it into the pulmonary artery and aorta. 2. Arteries, with elastic and muscular walls, form- ing channels for the blood to the system, assisting the heart in maintaining the circulation, and regulating the supply of blood to different parts. 3. Capillaries. — Canals of minute calibre, with thin permeable elastic walls, allowing both liq. san- guinis and white corpuscles to pass through their walls into the surrounding tissues. 4. Veins, forming channels back to the heart, provided with muscular walls and valves, and being sufficiently capacious to hold the total blood of the body. THE HEART The heart consists of four chambers with con- tractile walls, situated in the chest, and surrounded by a fibro-serous sac — the pericardium — in which it works. The Pericardium. — This membranous sac is attached below to the diaphragm, while its upper and narrower part surrounds and is attached to the great vessels connected with the base of the heart. ' It consists of an external fibrous layer, and an internal serous sac. The fibrous layer is a tough, dense 88 The Circulation membrane, attached below to the central tendon and muscular fibres of the diaphragm ; above it is at- tached to the great vessels, and is continuous with their external coats. The serous covering consists of a parietal layer, which is united to the inner surface of the fibrous layer, and a visceral, which is reflected round the great vessels enclosing the aorta and pul- monary artery in a common sheath. In structure the serous layer resembles other serous membranes. General Description of the Heart. — In form, the heart resembles a cone, its base being directed upwards, backwards, and to the right, its apex downwards, forwards, and to the left. In part it is covered by the lungs, especially during inspiration. Its apex-hesX is felt at the fifth intercostal space, two inches below the nipple, and one to the inner side of the left nipple line. In order to map the outline of the heart on the chest-wall, define the base by drawing a transverse line across the sternum corresponding with the upper border of the third costal cartilages, continuing it i inch to right of sternum, and i inch to left. Loiver border. — Draw a line from the apex- beat through the sterno-xiphoid articulation to the right edge of sternum. Right border. — Continue last line with an outward curve to join the right end of the base line. Left border. — Draw a line curving to left (inside nipple) from the apex-beat to the left end of the base line. Cavities of Heart. — The heart contains four chambers, two auricles and two ventricles. The Right Auricle receives the blood from the superior and inferior venae cavse at its upper and lower posterior angles. The septum between the two auricles forms the posterior wall, and presents the fossa ova/is (fig. 41, 3'), the remains of the foramen ovale, which is surrounded by a border (except below), the annulus ovalis. Between the two orifices of the Cavities of the Heart 89 venae cavse is the tubercle of Lower (fig. 41, 3), and in front of the opening of the inferior vena cava is the m_S: Fig. 41. — The right auricle and ventricle opened and part of the wall removed so as to show their interior (Quain's Anatomy), i, superior vena cava ; 2, inferior vena at the place where it passes through the diaphragm ; 2', the hepatic veins cut short ; 3, tubercle of Lower ; j, fossa ovalis, the Eustachian valve is just below ; 3", opening of the great coronary vein and valve ; 4, 4, right ventricle ; 4', large anterior columnar cornea ; 5, the anterior ; 5', the inferior ; 5", septal segment of the tri- cuspid valve; 6, interior of the pulmonary artery; 7, 8, aorta; 9, inno- minate and left carotid artery; 10, left auricular appendix; n, 11, left ventricle, 90 The Circulation Eustachian valve. The coronary vein opens into the auricle between the inferior cava and auriculo-ven- tricular opening, and is guarded by the valve of Thebesius (fig. 41, 3"). The auricular appendix is a tongue- shaped appendage, which projects from the anterior angle, and covers the root of the aorta. The cavity of the auricle is smooth, except that of the auricular appendix, which presents the muscular bands called musculi pectinati. The openings into the right auricle are the following : (1) Openings of vena? cavae ; (2) auriculo-ventricular opening ; (3) orifice of coronary sinus ; (4) openings of one or two small veins of right ventricle ; (5) foramina Thebesii, which are small depressions, some of them transmitting minute veins. The Right Ventricle forms the right border and chief part of the anterior surface of the heart. At its base are two orifices guarded by valves, the auriculo- ventricular and the pulmonary artery. The inner surface presents muscular elevations termed columna carnea, some of which are attached by their ex- tremities to the wall of the ventricle, others are at- tached along their whole length, while a third set are connected by their bases to the ventricular wall, and are connected by their other extremities to the seg- ments of the tricuspid valves, by means of the chordce tendinece (see fig. 41). The Left Auricle is situated at the posterior part of the base of the heart. It receives two pul- monary veins on each side, and opens into the left ventricle through the mitral valve. The interior of the left auricle is smooth like the right, its appendix presenting musculi pectinati. The Left Ventricle forms the left margin of the heart, the greater part of the posterior, and a small part of the anterior surface. Its walls are some three times as thick as the right ventricle, its musculi papil- Valves of the Heart 91 lares are larger, and the chordae stronger. Like the right ventricle, it has two orifices, auriculo-ventricular, guarded by the mitral, and the aortic, guarded by the semilzttiar valves. Endocardium. — The internal membrane lining the heart closely resembles the lining membrane of the arteries. It consists of a single layer of tesselated epithelium, with a connective-tissue layer beneath. Valves of Heart. — The mitral and tricuspid valves are situated at the auriculo-ventricular orifices, and prevent the passage of blood into the auricles during the ventricular systole. They consist of flaps or cusps, two in the mitral, and three in the tricuspid, connected by their bases to the auriculo-ventricular orifices ; their free margins and lower surfaces give attachment to the chordae tendineEe which connect them with the musculi papillares. They are formed of a duplicature of the lining membrane of the heart, strengthened by connective tissue. During the ven- tricular systole, the pressure of the blood in the ventricles presses their free edges, or rather their marginal surfaces, together, the musculi papillares regulating the tension of the chords and preventing the valves from becoming retroverted into the au- ricles. The semilunar valves guard the aortic and pul- monary openings. They consist of three semicircular folds attached by their convex margin to the wall of the artery at its junction with the ventricle, and are formed by a reduplication of the lining membrane strengthened by fibrous tissue. In the centre of each free margin is a little nodule, the corpus Arantii, the three meeting in the centre when the valves are closed. On each side of the corpora Arantii is a thin semilunar marginal surface, where the fibrous tissue is absent, called the lunula ; these surfaces come in contact when the valves close. After the systole of 92 The Circulation the ventricles, the tension of blood in the aorta and pulmonary artery closes the valves by distending them and pressing the marginal surfaces together. The semilunar valves during the ventricular systole are pressed back against the walls of the aorta, and hence, according to Briicke, prevent the filling of the coronary arteries which arise from the sinus of Valsalva during the ventricular systole, the coronary arteries being filled after the closure of the valves and during the diastole of the ventricle. Sounds of the Heart. — First sound. — Best heard at the apex-beat. It is synchronous with the ventricular systole, commencing immediately the ventricle begins to contract, but ceases before its completion (see fig. 42, outer ring). It is louder, longer, duller, than the second sound. Various explanations have been given as to its cause ; none of them are entirely satisfactory. It has been ascribed to : — (1) Closure of auriculo-ventricular valves ; (2) muscular sound of contraction of ventricles ; (3) car- diac impulse against chest-wall. The closure of the auriculo-ventricular valves seems the most probable cause, as when these valves are diseased the normal first sound is replaced by a murmur. The last explanation (3) is improbable. A systolic murmur or blowing sound is heard at the apex of the heart when the mitral valve is imperfect and allows of regurgitation. In ' button-hole ' mitral — that is, when the passage between the mitral valves is narrow so as to resemble a small button-hole — a presystolic murmur is heard, the murmur in this case being synchronous with the auricular systole. The second sound is short and sharp ; it is heard best at the junction of the third right costal cartilage with sternum, and corresponds to the closure of the semilunar valves, Between the first and second Sounds of the Heart 93 sounds the pause is very short, but between the second and succeeding first the pause is longer, and is about equal in duration to the time occupied by the first and second sounds together (fig. 42). In certain abnormal conditions of the aortic valves, when the valves fit together imperfectly and allow of re- gurgitation, ' double ' murmurs are heard at the base of the heart, replacing the first and second sounds. Fig. 42. — Diagram illustrating sequence of events in a cardiac revolution. The roughened valves both obstruct the blood-current and allow also of regurgitation, and thus an abnormal sound is produced during both systole and diastole. Doubling, or reduplication of the first or second sound, is produced by the two sides of the heart not acting at the same moment ; this condition is sometimes present- in Bright's disease, when the tension is abnormally high in the arterial system. Normally the aortic valves close £ s to ¥ V sec. before the pulmonary valves, on account 94 The Circulation of the greater tension in the aorta (see p. 97). Accord- ing to Potain, normally there is a reduplication of the second sound at the end of inspiration and beginning of expiration. The sounds have been likened to the pronuncia- tion of the syllables lubb, dup. A Cardiac Revolution.— A complete cardiac cycle includes : (1) systole of the auricles ; (2) sys- tole of the ventricles ; and (3) a passive interval (see fig. 42). (1) The auricular systole commences in the muscular fibres surrounding the great veins, the contraction running through vessels and auricles in a peristaltic wave, emptying the contents of the vessels into the auricle, and then emptying the auricle itself, the appendix being the last part to contract, the ventricles be- coming filled. Regurgitation into the great veins is ^hindered by (a) Peristaltic contraction of the muscular walls of the veins, their mouths becoming narrowed ; (b) Aspirating power of thorax during inspiration ; (c) Valves at junc- tion of subclavian and internal jugular veins. Regurgitation into the coronary sinus is pre- vented by the valve of Thebesius. (2) Then follows immediately the systole of the ventricles. The ventricles become tense and hard, change from a rounded to a more conical form ; the heart twists on its long axis from left to right, and ejects the ventricular contents — 5 to 6 oz. of blood — into the aorta or pulmonary artery ; the auriculo- ventricular valves close at the commencement of the Fig. 43. — Transverse sec- tion through the middle of the ventricles of a dog's heart in diastole and in systole (Ludwig.) A Cardiac Revolution 95 ventricular systole, while the semilunar valves open. The ventricular systole is not so simple as it at first sight appears ; the first stage or act consists in the sudden hardening of the ventricular walls at the com- mencement of their contraction ; the second is the forcible ejection of their contents, followed by closure of the semilunar valves ; thirdly, there succeeds a quiescent period, when they remain empty and con- tracted. The first stage, which is synchronous with the ' cardiac impulse,' is registered on the cardio- a le & a' h' Fie. 44. — Tracing obtained by a cardiograph placed directly on the ventricle of a cat's heart (Foster), a — b, corresponds to the distension of the ven- tricle, during the auricular sj'Stole ; b — c, the time during which the ven- tricles change their shape from a flattened to a rounded form ; e, marks the closure of the auriculo-ventricular and opening of the semilunar valves ; c — d, the expulsion of the ventricular contents, and time during which the ventricle remains contracted ; d — a', relaxation of the ventricles. The semilunar valves probably close atyC graphic tracing (fig. 44) by the sudden rise of the lever, b e ; the other two are included in that part of the tracing between e and f. The semilunar valves open and the auriculo-ventricular close at e, at the commencement of the active contraction, and the semilunar valves close and the auriculo-ventricular open at f, at the end of the systole. The time oc- cupied by a complete beat is about "8 sec. It will be 96 The Circulation divided in the following manner, supposing that the heart is beating at 60 per minute : — Contraction of auricles = -!- sec. Dilatation of auricles = f- sec. Contraction of ventricles = -f sec. Dilatation of ventricles = f sec. or, Auricular systole = \ sec. Ventricular systole = % sec. Pause = -f sec. (See fig. 42.) Some observers make the auricular systole occupy •j' v sec. instead of i. Thus : — Auricular systole = T V sec. Auricular diastole = T 9 a sec. Ventricular systole = x 4 - sec. Ventricular diastole = -^ sec. (3) The passive interval which follows the ventricular systole corresponds with the auricular diastole, blood pouring from the great veins into the auricle. Cardiac Impulse. — The impulse of the heart may be both seen and felt in the fifth intercostal space, midway between the left edge of the sternum and a line drawn vertically through the nipple. It is most marked during expiration, and disappears or diminishes at the end of inspiration, inasmuch as the heart's apex is separated from the chest-wall by lung. It is caused by the sudden hardening of the left ventricle during the systole, and probably also in part by the heart twisting slightly on its axis, the apex being brought more forward. A tracing can be obtained from the cardiac im- pulse in a man by means of a cardiograph. Fig. 45 shows the curve registered by the impulse of the Work done by Heart 97 heart of a healthy man ; a b corresponds to the auri- cular systole, the ventricles being filled with blood ; b c, c e, correspond with the apex-beat and ventricular sys- tole ; d and e mark the closure of the aortic and pulmonary valves, the aortic closing J n sec. before the pulmonary ; e / marks the diastole of the ventricles. Work done by the Heart. — It has been calculated that the daily work of the right ventricle, is equal to 15,000 kilo- gramme-metres ; the left ventricle is equal to 60,000, so that the total work of the heart in twenty-four hours may be estimated at 7 5, 000 kilogramme-metres, or about one-fifth of the total work performed by the body. This is about the amount of work performed by a man in the ascent of Snowdon (Foster). Frequency of Cardiac Pulsations.— In the adult, 65-75 (average 72) per minute. In the foetus, 150-200. At birth it is 140 ; end of second year, no; end of fifth, 100 ; end of fourteenth, 86 ; at twenty-one, 75. It is affected by position, being five beats more when sitting than lying down, and ten more in standing than sitting, a result due to the greater number of muscles brought into a state of contraction. The number of beats is increased by active exercise, during digestion, and by excitement. Increased resistance to the flow of blood at first in- H Fig. 45.— Tracing obtained by cardiograph from apex-beat of a healthy man. ad, contraction of the auricles ; b c, contrac- tion of ventricles ; d, closure of the aortic ; e } closure of pulmonary valves ; cf, diastole of ventricles. (Landoisand Stirling.) 98 The Circulation creases, then, if continued, diminishes the number of beats. Diminished pressure, as in a large hemorrhage, increases the number of beats. When the heart beats faster the duration of both systole and dgistole is diminished, but the diminution is most marked in the diastole. Normally the systole or working time of the ventricles is equal to about nine hours out of the twenty-four, and the rest is equal to fifteen hours. When the heart-beats are increased in number, as in the fevers, the total systole or work of the heart in twenty-four hours is increased as compared with the total resting time. Thus, if the heart beat 100 times per minute, the systole will amount to nearly eleven hours and the diastole to thirteen hours (Waller). Endocardial Pressure.— Goltz and Gaule found the maximum pressure in the left ventricle of a dog amount to 140 mm. of mercury, 60 mm. in the right ventricle, and 20 mm. in right auricle. Imme- diately after the systole a negative pressure of — 52 to — 20 mm. was observed in the left ventricle, in the right ventricle about —17 mm., and in the right auricle —12 to — 7 mm. While to some extent this negative pressure is due to the aspirating power of the thorax during inspiration ; yet, as a considerable nega- tive pressure is observed after the chest is opened, it would appear that the suction-power or active dilata- tion of the ventricles, and in a lesser degree the auricles, is of considerable service in carrying on the venous circulation. Innervation of the Heart The nervous mechanism of the heart consists of — i. Intra-cardiac ganglia. 2. Extra- cardiac — (a) inhibitory centre, (b) accele- rating centre. Cardiac Ganglia 99 3. Inhibitory nerves, i.e. vagi. 4. Accelerator nerves, i.e. sympathetic. 1. Intra-cardiac Ganglia —Automatic Ac- tion. — If the heart of a mammal be removed from the living body it will continue to pulsate for a few mo- ments, but the movements quickly cease, though they can be prolonged for a short time if some arrangement be made for supplying it with arterial blood. If a frog's heart be removed from the body it will continue to beat for hours, or even days, if it is kept moist, or, better still, supplied with a fluid containing O and also some nutrient fluid, as serum-albumin. It has been as- sumed that this automatic action of the heart is the result of the motor ganglia which the heart contains. Left auricle and pulmo- v, Superior venae cava; and nary veins i ) {\ . J vagi nerves Aortic bulb ~\^-*/ J_^^£^_^/ Sinus venosus and Re- V \^ZF^ /~~ mak's ganglia 7 * *V^ ( Inferior Bidder's ganglia ? ^^\ \ J interior vena cava Ventricle \ / Fig. 46. — Diagram of the frog's heart. This, however, is not universally true, as when a liga- ture is placed between the sinus venosus and the rest of the heart (auricles and ventricle), the latter part ceases to beat, although it contains Bidder's ganglia (see figs. 46 and 47). Moreover, parts of the heart, such as the venae cavas and upper part of the sinus venosus will pulsate when separated from the rest of the heart, though no ganglia have been demonstrated in them ; and the heart of the snail contains no nervous elements, yet it beats rhythmically. It would seem that the property of rhythmical contraction is an attribute of heart muscle. While it is unsafe, in the present state of our knowledge, to make any dogmatic statement about the functions of the cardiac ganglia, loo The Circulation it would seem they are capable of originating rhythmical pulsations and retaining this power after the muscular fibres themselves have ceased to con- tract automatically. The ganglia appear also to be more sensitive to outside stimuli than the muscular fibres. The principal facts known concerning the cardiac ganglia have been studied in the frog's heart. The frog's heart consists of two auricles above and a single ventricle below ; the latter is continuous in front with the aortic bulb, which divides into two aortas — right and left. Pos- teriorly the right auri- cle receives the sinus venosus, a small cham- ber formed by the junction of the two r , . superior venae cava; Jig. 47. Scheme of nerves or frogs x , . r heart (Landois and Stirling), r, Re- and inferior vena cava. maf S) and b, Bidder's ganglia ; s v, Thecardiac branches sinus venosus; A, auricles; V, ven- tricle;. IS A, bulbus arteriosus; Vag, of the Vagi paSS to the vagi; I V C, inferior vena cava; .:_„,. ..„,.„,,,, whprp S V C, superior vena cava. SlnUS \ UIOSUS, Wliere they are connected with some nerve-cells which form Remak's ganglia; branches proceed along the auricular septum to two ganglia situated in the auriculo-ventricular groove, called Bidder's ganglia (see figs. 46 and 47). The most important experiments in connection with the ganglia of the frog's heart are the following : — Stannius's Experiment.— If the sinus venosus be separated from the auricles by tying a ligature round the line of junction between the two (see fig. 48, 1), the sinus venosus and veins continue to beat, while the auricles and ventricles stand still in diastole. If an incision be made at the auricular- ventricular groove, so as to separate the ventricle from the auricles, Bidder's ganglia being included with the ventricle, the ventricle commences to pulsate again. Extra-cardiac Centres 101 Thus the sinus venosus and ventricle are pulsating, though with a different rhythm, while the auricles are motionless (see fig. 48, 2). This experiment has been thought to prove that both Bidder's and Remak's ganglia are motor in function, while the auricles contain in- hibitory ganglia. Section of the Heart— If the ven- Y Vw^ tricle be Separated Fir.. 4 S.— Stannius's expeiiment (Landois frnm the- nnrir]pu Vw a,Hi Stirling). A. auricle ; V, ventricle ; irom tne auriCleb Dy sv , s i„ us venosus; the zigzag lines in- meanS Of SCisSOrS, the dicate which part continues to beat ; in , i • j 7 7 2 the ventricle beats at a different rate. cut being made below the auriculo-ventricular groove, so that the upper part of the ventricle goes with the auricles, Bidder's ganglia being included, the auricles will continue to beat rhythmically, while the lower part of the ventricle is motionless. If the heart be divided longitudinally, each half — an auricle and half- a ventricle — will pulsate. 2. Extra-cardiac Centres. — The inhibitory (vagus) centre is situated in the medulla, and is con- stantly in action. It is capable of being influenced by the excitation of various sensory nerves. The accele- rating centre is also in the medulla ; it is not constantly in action. These centres are largely influenced by afferent nerves from various parts of the body. Thus a ghastly sight, good news, an inflamed pericardium or peritoneum, may profoundly influence the pulsations of the heart through its regulating centres in the medulla. 3. Inhibitory Action of Vagus —If the vagus of a frog or rabbit be excited by an interrupted current, the heart's action will become slower, and the blood-pressure in the arteries will be diminished ; or if the current be strong, it will be arrested in diastole. The researches of Roy and Adami show that even weak excitation of the vagus reduces considerably the 102 The Circulation amount of blood passing through the heart, by reducing the force and frequency of the cardiac beats. A strong excitation will stop the contraction of the auricles and keep them quiescent ; it will temporarily arrest the ventricles, but the ventricles will recom- mence their contractions in spite of the continued excitation of the vagus. Section of the vagi is VACUS CENTRE ACCELERANS CENTRE /NH/BITORY CA/VCL/ON, ACCELERATING GANGLION Fig. 49.— Diagram illustrating the connexions of the inhibitory and accelerator} 7 nerves of the heart. followed by an acceleration of the cardiac beats. If atropin be injected, even a strong current passed along the vagi will not diminish the cardiac beats. Reflex Inhibition. — If the intestines of a frog be struck sharply, or the mesenteric nerves stimulated, the heart is brought to a standstill in diastole. If the The Arteries 103 vagi are divided, or the medulla destroyed, this effect will not take place. The stimulus ascends to the medulla along the mesenteric nerves, and descends to the cardiac ganglia along the vagi. Irritation of other sensory nerves, as the posterior auricular, will have a similar effect. 4. Accelerator Nerves. — The sympathetic nerves which pass from the cervical cord to the last cervical and first dorsal ganglia, and from thence to the heart, are called the accelerator nerves. Stimu- lation of these nerves with the interrupted current causes quickening of the heart's action, and causes it to beat with greater force. The output of blood is also increased. The accelerator nerves exert no influence on the heart if the cardiac branches of the 'vagus have been paralysed by section or by atropine. It would thus appear as if the accelerator nerves act as a check on the vagus (Roy and Adami). Passing along with the accelerators are some branches which are vaso-constrictors for the coronary arteries. THE ARTERIES Structure. — The arteries have three coats — I Epithelial. t. Internal j Sub-epithelial. 1 Elastic. ,,.,-,, I Muscular. 2. M.ddle |E]astic _ 3. External — Connective tissue. 1. Internal (fig. 51, a, I?).— This coat may be readily stripped off the inner surface of the artery as a transparent, colourless, elastic and brittle membrane. It is formed of — (a) An epithelial layer, consisting of a single layer of thin, elongated cells, with nuclei (fig. 50). io4 The Circulation (b) Sub-epithelial layer, composed of branching corpuscles lying in cell-spaces of homogeneous con- nective tissue, (c) Elastic layer, consisting of a fine membrane marked with interlacing network of fibres and per- forated with round openings, and termed fenestrated membrane of Henle (fig. 5 1 b). 2. Middle or Muscular (fig, 51, c)— In the small and medium-sized arteries the middle coat consists of pure non -striated muscular fibre, arranged transversely round the artery with only a slight admixture of elastic tissue. In the larger arteries yellow elastic fibre predominates, and, indeed, the aorta consists of nearly pure yellow elastic tissue. 3. External coat, or tunica adventitia (fig. 51, d), consists ot fine connective tissue, with a vari- able amount of elastic tissue ar- ranged longitudinally. Circulation in the Arteries. The arteries are elastic and con- tractile tubes which convey the blood from the heart to the capil- laries. The larger arteries are ex- ceedingly elastic, but feebly con- tractile ; the small arteries are contractile on account of the mus- cular tissue in their walls, while they are less yielding and elastic than the larger arteries. Their elasticity allows them to dilate during the systole of the heart, thus diminishing any risk of rupture, and their elastic recoil during the diastole, when the aortic valves are closed, assists in maintaining the circulation, and con- Fig. 50. — Epithelial layer lining the pos- terior tibial artery of man x 250 (Qnain's Anatomy). The Arteries 105 verts what would be an intermittent supply of blood to the capillaries into a constant stream. The contractile power possessed by the smaller arteries is of great importance (1) in regulating the supply of blood to an organ. Thus during digestion the minute gastric arteries dilate and supply the peptic glands with a larger supply of blood than during fasting. The arterial muscular tissue is regulated by the vaso-motor nerves. (2) It assists in arresting haemorrhage, when an artery is completely divided, by occlusion of the Fig. 51.— Transverse section of part of the wall of the posterior tibial artery x 75 (Quain's Anatomy). a, epithelial and sub-epithelial layers ; b, elastic layer of inner coat ; c, muscular layer ; d, outer coat, consisting of connective-tissue bundles. divided ends. (3) It enables the arterial system to accommodate itself to the amount of blood in the body. At each ventricular systole some 5 oz. of blood are forced into an already overfilled aorta and arterial system ; the effect of this being (1) to increase the tension in the arterial system and distend the elastic walls of the aorta and large arteries, (2) to send a wave-impulse along the blood in the arteries, which is gradually lost before reaching the capillaries, and which can be felt in the radial as the pulse. If the arteries were rigid tubes, the intermittent action of the heart would cause an intermittent flow of blood from the arteries to the capillaries. The effect of the ven- tricular systole is to distend the walls of the aorta, to store up force during the systole to be utilised in io6 The Circulation continuing the circulation during diastole, the recoil of the elastic walls assisting to convert the intermittent blood-stream into a continuous one. If a large vessel, as the carotid, is divided, an intermittent stream of blood flows out, but a wound of a small artery yields a steady stream. Arterial Pres- sure. — The pressure of blood in the arteries is measured by con- necting the carotid artery of a rabbit or dog with a |j -shaped tube containing mer- cury. If afloat on- the mercury be made to carry a small camel's- hair brush or pen, the oscillations of the mer- cury caused by the varying tension in the blood-vessels can be recorded by the brush or pen writing on a re- volving surface. Such an arrangement is called a kymograph. The pressure in the arteries undergoes vari - ations which correspond — (i) with each systole of the left ventricle, (2) with the movements of" respiration (see p. 141). The mean pressure in the carotid of man probably amounts to about 150-200 mm. (6-8 in.), in the aorta 250 mm. (9-8 in.), and in the brachial 1 10-120 mm. (4-3-47 in.) of mercury. Fig. 52.- Transverse section through a small artery and vein (Gray's Anatomy). A, artery ; V, vein ; e, epithelial lining ; 111, circular muscular fibres with nuclei ; «, external or connective tissue coat. The Arteries 107 The arterial pressure or tension decreases in pass- ing from the larger to the smaller arteries, in spite of the increased friction which results from the blood travelling through smaller channels. The reason for this decrease in pressure is to be found in the fact that the total sectional area of the smaller arteries is greater than the trunk from which they were derived, and the blood is therefore travelling as it were through a wider stream. The blood-pressure varies under different circumstances, depending upon three factors : (1) the force and frequency of the heart's contrac- tions, (2) the elasticity and tone of the arteries, (3) the resistance in the capillaries. The force of the heart and tonus of the arteries may vary to suit altered conditions of the circulation. Thus, ligature of the large arteries or an injection of saline fluid into the circulation (when the pressure is normal) leads to only a transitory rise in the pressure, the pressure quickly falling to normal. The injection of a greater volume of fluid than is equal to the volume of the blood in the body leads to high pressure and death. A moderate haemorrhage, less than 3 per cent, of the weight of the body, does not lower the pressure ; more than this leads to a lowering, and perhaps death. Velocity of the Flow. — The rate of movement of the blood in the arteries has been measured prin- cipally in the carotids of the horse, dog, and rabbit. In the horse Volkmann found the velocity to be 300 mm. per sec. in the carotid, 165 mm. in the maxillary, and 56 mm. in the metatarsal. Various instruments are employed for this purpose, the Stromuhr of Ludwig and the Hamatachometer of Vierordt being the principal. In order to measure the time occupied by the circulation of any portion of blood, ferrocyanide of potassium is injected into the jugular vein, and the blood from the peripheral io8 The Circulation end of the same vein tested from time to time. In this way a complete circulation has been found to take place in 15 sees, in the dog, and 23 sees, in the human subject {Hermann). The Pulse. — The impulse or shock caused by the overfilling of the aorta during the ventricular systole is the cause of the pulse. This pulse-wave travels at the rate of 5-10 metres (15-3° ft.) per second along the arteries, and is lost at the capillaries. This pulse-wave must be carefully distinguished from the blood-current, the latter travelling only some 300 mm. (12 in.) per second; the former stands in the same relation to the moving blood as does a wave on the surface to the cur- rent of a slowly flowing river. The duration of the ventricular systole being fths second, before the end of the systole the pulse-wave would have travelled about 12 ft., if that were possible, so that the beginning of each wave is lost at the peri- phery before the end of it has left the ventricle. The more rigid the arteries the faster the wave travels ; the more distensible the more slowly it travels. If the finger be applied to the radial artery, the artery will be felt to expand beneath the finger some 75 times a minute ; under some circumstances the pulse- wave will feel to be double or dicrotic. This di- crotism is shown by the sphygmograph to be constant in health, but is more marked when the tension in the arteries is low, and the arterial walls more dis- Fig. 53. — A normal pulse-trace mag- nified, a li c, primary wave ; c d e, predicrotic wave; efg, di- crotic wave ; e, aortic notch ; a— e, systole ; /— a', diastole of the ventricles. Pulse 109 / Fig. 54. — Tracing of a dicrotic pulse (fever). tensible than usual, as in febrile conditions of the system. In a sphygmographic tracing, fig. 53, the up-stroke a — b is caused by the pulse-wave as it travels along distending the artery and raising up the lever of the sphymograph. The down-stroke b—a' is more gradual than the up-stroke, and is caused by the descent of the lever consequent on the artery regaining its normal calibre. The descent is marked by several minor waves. The largest of these, efg, is called the dicrotic wave, and is due to a wave or vibration sent along the arteries by the sudden closure of the aortic valves. The cause of the other minor waves, the pre- and post-dicrotic, is uncertain. The character of the pulse as regards frequency, tension, &c, is modified by changes occurring (1) in the beat of the heart, (2) or changes taking place in the arterial walls or capillaries. Thus in the low ten- sion of some fevers, due to a relaxed con- dition of the arterial system, there is a sharp, short ventricular contraction, giving a straight up-stroke (fig. 54, a — b) to the primary wave, and a prominent dicrotic wave, efg. The same result occurs after a warm bath, the arteries relaxing and the tension becoming low. In Bright's disease the tension is high and the ascent of the primary Fig. 55. -Yortic regurgitation (Landois and Stirling). 1 1 o The Circulation wave is gradual, and the secondary waves small. The same effect is produced by a cold bath. In aortic regurgitation (fig 55) the up-stroke is sudden and high, in consequence of the dilatation and hypertrophy of the left ventricle, a larger volume of blood than usual being propelled into the arteries ; the down-stroke is abrupt and the dicrotic wave very slightly marked on account of the imperfect closure of the aortic valves. The characters of the pulse which are of most importance clinically are (1) frequency, (2) compressi- bility, (3) rhythm. The terms large or small, strong or weak, are also used, but are of less importance and apt to be misleading. The pulse-rate is easily ascertained with the finger on the radial artery and watch in hand. The relative compressibility may be ascertained by pressing the finger on the artery ; the pulse may be felt to be liard or soft. A hard, slow pulse signifies high arterial tension, as in Bright's disease ; a soft, quick pulse, low tension, as in fevers. In such cases the pulse is usually markedly dicrotic. The rhythm may be altered so that the beats are irregular or are intermittent. Pulse-rate. — In health the normal pulse-rate in an adult is 70 in a male and 80 in a female ; in the newly-born it is 130-140 ; at three years of age it has fallen to 100 ; at ten years of age to 90 ; at twenty- one years to 70 ; after middle-life it is slightly higher. THE CAPILLARIES Structure. — The smaller arteries end in a fine network of vessels, which differ in structure from the arteries and veins, in that their walls contain no muscular elements, but consist of a single layer of elongated epithelium continuous with that of the arteries ; the epithelium is rendered apparent by The Capillaries 1 1 1 injection of solution of silver nitrate, and exposing to light, the reagent darkening the intercellular material and rendering the outline of the cell apparent (fig. 56). The nuclei can be stained with logwood. In the vessels slightly larger than the capillaries a layer of elongated muscular fibre cells is added. Size. — Their average size in the human body is about -joVoth of an inch, but they differ in different parts of the body. They are comparatively large in the marrow of bone, skin, and mucous membrane ; small in lung, muscle, and brain. The net- work is close in lung and muscle. Circulation in Capillaries. — The velocity of the blood in the capillaries is very much less than in arteries or veins, being about '57 mm. to 75 mm. per second ( 1 '4-1 - 8 inches per minute). But a very small portion of the capillary system is tra- versed by any one blood-corpuscle. The flow is constant, not intermittent, as in the larger arteries. Under some abnormal circumstances, as in hypertrophy of the left ventricle and a rigid condition of the arteries, an in- termittent flow or pulse may occur in the capillaries. This may be demonstrated by pressing the finger on the forehead ; alternations of redness and pallor are noted lit the border of the pressure-mark. The red blood-corpuscles, for the most part, travel in the Fig. 56. - Capillary vessels from the bladder of a cat (Chrzonszczewsky). 1 1 2 The Circulation mid-stream, the white corpuscles moving more slowly along the side. The thin capillary walls allow the liq. sanguinis readily to pass through, and so bring the blood in direct contact with the tissues, and also nourish parts by irrigation in which there are no capillaries, as cartilage and the cornea. Under certain circumstances, as in inflammation, when the capillaries are distended, the white corpuscles push through the capillary wall into the tissues passing through the intercellular substance between the en- dothelial plates. This is termed Diapedesis. The capillary circulation can be readily seen when the web of a frog's foot is spread out beneath the microscope. It may also be seen, suitable precautions being taken, in the mesentery of some of the smaller mammals. The capillary walls, though they contain no muscular element, are apparently contractile. The calibre of the capillary channels is distended when a large supply of blood reaches the part, and the channels shrink when the supply is less. It appears that some- times they can change their form independently of any engorgement with blood, the endothelial cells which form their walls being apparently slightly con- tractile. The movement of blood in the capillaries is dependent upon the action of the heart, modified by the arteries. Veins Distribution.— The veins carry the blood from the capillaries to the heart. They ramify through the body like the arteries, but they are more numerous, anastomose more freely, and are of greater capacity. They usually accompany the arteries ; but there are exceptions, as the hepatic, sinuses of the skull, and veins of spinal cord. Structure. — The veins have thinner walls than the arteries. They have the following coats :— The Veins ii3 1. Internal. — This coat closely resembles the inner coat of the arteries (fig. 57). 2. Middle.— This coat is thinner and less mus- cular and contains more white fibrous tissue than the middle coat of the arteries. The muscularity of the middle coat is best marked in the splenic and portal, and least marked in the hepatic part of the inferior vena cava and subclavian veins (fig. 57). 3. External. — This coat consists of connective tissue and elastic fibres. In certain veins this coat contains a considerable quantity of muscular tissue, as in the abdominal cava, iliac and renal. The FlG.57. — Transverse section of part of the wall of one of the posterior tibial veins (Schafer). a, epithelial and subepithelial layers ; b, elastic layer of inner coat ; c, middle coat of muscular and connective tissue ; d, con- nective-tissue coat. striated muscular fibre of the heart is prolonged for some distance on the walls of the pulmonary veins and venae cavse. Muscular tissue is wanting in most of the veins of the brain and pia mater, retina, venous sinuses of dura mater, and cancellous veins of bone. Valves. — The valves consist of semilunar folds of lining membrane, strengthened by including con- nective tissue. They consist for the most part of two flaps or pockets, which come in contact by their free margins, and prevent reflux of blood towards the capillaries. The veins of the extremities, neck, and 1 1 1 4 The Circulation scalp have, numerous valves, while they are absent for the most part in the deep veins of the abdomen, chest, and cranium. Many other veins are destitute of valves. Such are the vena? cavse, portal, hepatic, renal, uterine, pulmonary, and sinuses of skull. There are a few in the intercostal and azygos. The forces which propel the blood in the veins are — i. Vis a tergo — heart's action. 2. Vis a fronte — aspiration of the thorax. 3. Muscular contraction. (1) The vis a tergo or force exerted by the heart in assisting the flow of blood in the venous system is probably not great, the velocity of the blood in the small veins being small. (2) The vis a fronte or force supplied by the suction action of the chest during inspiration is much more considerable. When an ordinary inspiration is taken, not only is air drawn into the air-passages by the expanding chest, but the blood in the great veins external to the chest is sucked towards the right auricle. The effect is more powerful if a deep in- spiration is taken. During an ordinary expiration the sucking action becomes nil, while during a powerful expiration, as in blowing or coughing, the expiratory effort obstructs the flow of blood into the chest and causes congestion of the venous system. (3) During muscular exercise the veins are com- pressed by the contracting muscles, the effect being to drive the blood towards the heart, the valves pre- venting its return towards the capillaries. The velocity of the blood in the venous system is small when compared with the arteries, though greater in- the large veins near the heart than in the smaller veins. It is about 200 mm. per sec. (7 to 8 inches) in the jugular vein of the dog. The pressure Vaso-motor Centres 115 in the crural vein of the sheep has been shown to be 1 1 "4 mm. of mercury (-4 inches), while in the sub- clavian it was —1 mm. to —5 mm. during inspiration, the mean pressure being — -i mm. Venous Pulse.— The flow of blood in the veins is, unlike the flow in the arteries, continuous and not intermittent. In the large veins, however, the aspi- rating power of the thorax draws the blood to the chest during inspiration, and thus leads to more or less intermittency. In case of regurgitation through the tricuspid valve, there is a ' back-stroke ' seen in the veins of the neck, sometimes called a 'venous pulse.' Innervation of the Blood-vessels 1. Vaso-motor centres — medulla, cord, ganglia. 2. Vaso-motor nerves, i.e. (a) vaso -constrictor, (b) vaso-dilator. Vaso-motor Centres.— The principal vaso- motor centre is situated in the medulla. Nothing is known of this centre anatomically, its position having been determined by experiment. Excitation with the interrupted current of the medulla of a frog will cause the vessels in the web of the foot (when seen beneath the microscope) to contract. The same result can be witnessed in the rabbit by exposing a small artery. Section of the cord below the medulla causes the vessels to dilate. The latter experiment shows that the muscular fibre of the arteries is in a continual state of contraction or tonus. Various subsidiary vaso-motor centres are situated in the spinal cord. Besides the vaso-motor, or rather vasoconstrictor nerves, there are vaso-inhibitory or vaso-dilators. Such are the chorda tympani to vessels of the submaxil- lary glands, and the nervi erigentes to the arteries of the erectile tissue of the penis. The vaso-motor centre 1 2 1 1 6 The Circulation can also be influenced by various afferent nerves : this may occur through the higher nerve-centres, as in blushing ; excitation of the central end of various sensory nerves will bring about contraction of arteries ; while the vagus contains, especially in the superior laryngeal branch, fibres which excite and also fibres which, when stimulated, lead to inhibition of the vaso-motor centre. Action of Poisons on the Heart and Circulation Muscarin, an alkaloid extracted from a poisonous mushroom, injected into the circulation, slows the heart and finally arrests it in diastole. It is assumed to stimulate the inhibitory ganglia of the heart. Pilocarpin has the same effect. Atropine increases the frequency of the heart-beats, and appears to paralyse the inhibitory ganglia, thus antagonising muscarin. Excitation of the vagus has no effect. Nicotine, like atropine, paralyses the inhibitory ganglia. Physostigmin, or Eserin, in small doses increases the effect of stimulation of the vagus, and to this extent antagonises atropine. Digi/alin. — The first effect is to slow the heart and give increased strength to the systole. In the second stage the heart becomes irregular in action and in- creased in frequency ; finally the heart is arrested in systole. Aconitin and Veratrin slow the heart by exciting the vagus. Alcohol and Etlier in small doses stimulate the heart, so that it beats more strongly and more quickly. Origin of Lymphatics 1 1 7 CHAPTER IX LYMPHATIC SYSTEM Distribution. - The lymphatic vessels may be said to take their origin in every tissue of the body supplied with blood ; they carry back into the vas- cular system any excess of the plasma of the blood which has transuded from the capillaries, and which is not required for the nutrition of the tissues. The lymphatics or lacteals which originate in the mucous membrane of the alimentary canal, perform the im- portant office of taking up certain of the products of digestion and conveying them into the vascular system after their passage through the mesenteric glands. The lymphatic capillaries commence in various ways in the tissues ; by their junction they form the larger lymph-vessels ; these finally join either the left or right thoracic duct, and by their means the contents of the lymphatic vessels enter the subclavian veins. Modes of Origin. — The lymphatics have varied modes of origin — (1) in plexuses or networks of capillaries ; (2) in lacuna or clefts in connective tissue ; (3) in lymph spaces or cavities. 1. Plexi- form. — Networks of capillary lymph-vessels are pre- sent beneath the skin, and mucous membrane of the stomach and intestines, some of the plexuses being joined by small blind vessels, as in the villi. 2. Lacunar. — Various spaces or interstices in the connective tissue of various organs are connected with the lymph capillaries. These clefts are generally without a complete endothelial lining, but the endo- thelial cells forming the wall of the lymph capillaries are directly continuous with the connective-tissue 1 1 8 Lymphatic System cells in the clefts or lacuna?. 3. Lymph spaces or cavities. — In some parts the lymphatic capillaries commence in spaces or sinuses lined by a single layer of squamous epithelium, or rather, endothelium, with sinuous outlines, similar to the endothelium lining the lymph vessels. Such are found beneath the skin and mucous membranes, in the diaphragm, lungs, liver, &c. Resembling these, only very much larger, are the serous sacs, such as the pleural, pericardial, and peritoneal cavities ; also the synovial cavities and the subdural and subarachnoid spaces. The cavities are directly connected with the lymphatics by minute holes — the stomata surrounded by a layer of poly- hedral cells. By means of these openings fluids and solid matters can enter the lymphatics. Lymphatic capillaries. — These consist of channels, for the most part larger than the capillaries of the vascular system, their walls consisting of a single layer of flattened nucleated epithelium, with sinuous outlines. Sometimes a small artery is com- pletely surrounded or ensheathed in a lymphatic capillary ; the space surrounding the artery is termed the perivascular lymph space. Lymphatic vessels. — The capillaries empty themselves into vessels which closely resemble veins. These are lined by a single layer of elongated nucleated flattened cells with sinuous outlines ; outside the epithelial coat is a thin layer of longitudinal elastic tissue, the middle coat consists of muscular tissue, and the external of a mixture of connective and mus- cular tissue. They are provided with valves so closely approximated as to give them a beaded appearance. The valves resemble those described with the veins. Thoracic duct. — All the lymphatics of the body, except those of the right side of the head, right thorax, right upper extremity, and right side of the heart, empty themselves into the thoracic duct. The Lymph 1 1 9 thoracic duct commences opposite the second lum- bar vertebra, this part being dilated and termed the receptaculum chyli, and terminates in the subclavian in the neck near its junction with the jugular. Functions.— Liq. sanguinis exudes from the capillary blood-vessels to supply the tissues with materials for their nutrition. The excess of liq. sanguinis thus supplied enters the lymphatic capil- laries, passes through the lymph glands into the thoracic duct, and thence into the venous circulation. The liq. sanguinis that has passed out of the capil- laries accumulates in the connective-tissue spaces, or lacunae, from which the lymphatics arise. The lymphatics which arise in the villi of the small intestines are termed lacteals, and during digestion absorb fatty matters, and to a smaller extent soluble matters and albumen from the contents of the intestine. During the digestion of food, the columnar epithelium covering the villi may be seen to be dis- tended with oil globules (though some observers assert the oil globules pass between the cells), these globules passing from the epithelium into the retiform tissue, and thence into the fine lacteal present in the villus. Lymph has been described as blood minus the red corpuscles. It is a yellow alkaline fluid of sp. gr. 1045 and 6-7 per cent, of solids. It consists of — White corpuscles. Extractives. Elements of fibrin. Salts. Albumen. Water. It has been obtained for examination from the thoracic duct during a fasting period, or from some large lymphatic vessel. The white corpuscles are more numerous in lymph which has passed through lymph glands. They vary in size ; the larger con- 120 Lymphatic System tain two or three nuclei and show more active amoeboid movements than the smaller ones. Chyle may be described as lymph plus fatty matters. It may be obtained from the thoracic duct during a period of digestion. It is an opaque milky fluid which clots when drawn from the duct : the clot exhibits a pink colour. It contains 8-9 per cent, of solids. It consists of — White corpuscles. Albumen. Immature red. Extractives. Fatty matters. Salts. Elements of fibrin. Water. Examined miscropically, white corpuscles are seen in abundance in chyle drawn from the upper part of the thoracic duct. Many of these white corpuscles are of a reddish colour, and are probably in process of being converted into red. The fatty matters consist of oil globules of various sizes and finely divided matter of a granular appear- ance, which forms the molecular basis of chyle. Chyle undergoes changes in its passage from the villi to the thoracic duct ; these changes are effected through the agency of the mesenteric glands. They consist in a diminution of the molecular basis and an increase of the white corpuscles and elements of fibrin. Some of the white corpuscles appear to be of a reddish colour. Movements of the Lymph 1. Vis a tergo. Pressure of blood in the blood- vessels. 2. Contraction of muscular fibres in their walls and in the villi. Lymphatic Glands 121 3. Compression by muscular action of voluntary muscles. 4. Vis a f route. Aspiration of thorax. 1. If a ligature be applied to the thoracic duct, the chyle will tend to accumulate behind it, or if a tumour compress it the lacteals will become dilated and tortuous. This shows the existence of some vis a tergo. The liq. sanguinis leaves the capillaries under considerable pressure, and accumulating in the spaces of the tissues readily passes into the lymphatic vessels. Increase of pressure in the arteries causes increased tension in the lymphatics. 2. The muscular fibres in the walls of the lym- phatic vessels act after the manner of the lymph- hearts in the frog. The contraction of the muscular fibres of the villi assists in emptying the contents of the contained lacteal. 3. Contraction of the voluntary muscles com- presses the lymphatic vessels in the same way as the veins, driving the lymph forwards, the valves prevent- ing reflux. 4. The enlargement of the chest during inspiration sucks the blood in the large veins towards the heart ; the rapid motion of the blood in the subclavian over the orifice of the thoracic duct will tend to make the contents of the duct discharge into the vein, thus sup- plying the vis a fronte. Lymphatic Glands.— The basis of all lymph- glands is the so-called lymphoid tissue, which consists of retiform or adenoid tissue with lymph corpuscles occupying the meshes of the network (fig 58). Lym- phoid tissue therefore consists of (a) a network of fine fibres ; (b) small nucleated cells at the intersections of the network, which may be separated from the fibrils of the network ; (c) lymph-corpuscles occupying the meshes. Some of the corpuscles are small with 122 Lymphatic System a large nucleus, and are probably the newest formed ; others are larger and probably older, with two or three nuclei, and exhibit more lively movements on the warm stage than the smaller ones. Lymphoid tissue, according to Klein, occurs in the body in the following ways — (i) Diffuse lymphoid Fig. 58. — Diagrammatic section of lymphatic gland (Sharpey). a i, afferent, c /, efferent lymphatics ; C, cortical substance ; M, reticulating cords of medullary substance ; Is, lymph-sinus ; c, fibrous coat sending trabeculse / r into the substance of the gland ; Ik, lymphoid tissue. tissue, which is found extensively beneath the epithe- lium of mucous membranes, notably of the trachea, soft palate, tonsils, root of tongue, pharynx, small and large intestines. (2) Cords, cylinders, or patches in Lymphatic Glands 12 • the pleura and spleen. (3) Lymph follicles, being oval or spherical masses, in the tonsils, root of tongue, upper part of pharynx, stomach, intestines (solitary glands), nasal and tracheal mucous membranes. In the Malpighian corpuscles of the spleen. Compound Lymphatic Glands.— These are the commop lymph-glands, which are small rounded bodies placed in the course of the lymphatic and Fig. 59. - Thin section from the cortical part of a lymphatic gland (Hi;.). ad, network of fine trabecular, formed by retiform tissue, from the meshes of which the lymph-corpuscles have been washed out, except at c, where they are left. lacteal vessels, and through which the lymph and chyle pass on their way to the thoracic duct. They are collected in groups, such as the mesenteric, portal, bronchial, splenic, cervical, lumbar and inguinal glands. The afferent lymphatics enter the glands on its outer or convex surface, and emerge as the efferent lymph-vessels at the hilum. Each gland is surrounded by a fibrous capsule which passes into the interior as the trabeculce or septa. The trabecular pass one-third or one-fourth of the way into the gland (the cortex), dividing it into oval compartments (fig. 58, tr), whilst in the central portion 124 Lymphatic System they join together and form small compartments of an irregular shape (medullary portion). The capsule and trabecular are formed of fibrous tissue and non-striped muscular tissue, and carry the vessels which enter the gland, and are distributed to the lymphoid tissue of the gland. The compartments of the cortex contain oval masses of lymphoid tissue which do not com- pletely fill them, and which form the lymph follicles of the cortex ; the medullary portion contains .irregularly shaped or elongated masses called the medullary cylinders (fig. -58, C, M). The space left free between the lymph follicles and the cortical trabecular, and between the medullary cylinders and the trabecular, forms the lymph sinus (Is). The lymph path or sinus is occupied by coarse retiform tissue. The afferent lymphatic vessels, having entered at the cortex at the external surface, open into the lymph sinuses of the cortex, then the lymph passes into the lymph sinuses of the medulla, and leaves the gland by the efferent lymphatics at the hilum. The passage of the lymph through the sinuses is delayed by the reticulum, and any foreign bodies or inflammatory products may be arrested in the passage. CHAPTER X RESPIRATION TRACHEA AND BRONCHI The walls of the trachea and two bronchi consist of several constituents — 1. Connective tissue. 3. Muscular. 2. Cartilages. 4. Submucous. 5. Mucous membrane. Trachea 125 1. The Connective Tissue coat forms an ex- ternal sheath for the trachea, surrounding and joining together the cartilages (fig. 60, /). 2. The Cartilaginous Rings are incomplete behind, being C-shaped, are 16-20 in number, consist Fig 60 —Longitudinal section of the human trachea (Klein), a, ciliated epithelium ; />, basement membrane ; c, superficial part of the mucous membrane, containing capillary vessels and lymphoid tissue ; d, deeper layer of mucous membrane, consisting mainly of elastic, fibres ; e sub- mucous tissue containing the larger blood-vessels, mucous glands and fat ;/, fibrous tissue, investing cartilages ; g, fat-cells ; A, cartilage. of hyaline cartilage, and serve to maintain a certain amount of rigidity in the walls. 126 Respiration 3. The Muscular Layer is present behind, connecting the tips of the cartilages together, and is also present behind in the intervals between the rings. Its fibres belong to the unstriated variety, and serve by their contraction to diminish the diameter of the tube. 4. The Submucous Coat (fig. 60, e) consists of loose connective tissue containing mucous glands, blood-vessels, and adipose tissue, and serves to con- nect the mucous membrane with the cartilages and their sheath. 5. The Mucous Membrane consists of from within outwards (fig 60) — (a) a single layer of columnar ciliated epithelium cells, with a few ' goblet cells ' ; {/>) a basement membrane ; (c) a layer of lymphoid tissue, with a network of capillary blood- vessels ; (d) a layer of longitudinal elastic fibres, collected into bundles, which are readily seen as longitudinal striae on slitting up the trachea. The bronchi external to the lungs exactly resemble the trachea in structure, the right having 6 to 8, the left 6 to 12 incomplete cartilaginous rings. Lungs (weight — right, 24 oz., left, 21 oz.) The lungs are surrounded by the pleurae, the smooth surfaces of the latter diminishing friction during the movements of respiration. In shape they are conical, the apex projecting into the root of the neck, the base resting upon the arch of the diaphragm ; the inner surface being flattened where the bronchi and vessels enter. The lungs consist of — 1. Lobes ; 2. lobules ; 3. bronchi ; 4. terminal bronchioles, alveolar passages and infundubila ; 5. air-sacs : 6. blood-vessels and nerves. Lungs 127 1. The Lobes are the primary divisions, the right having three, the left two. 2. Lobules. — The lobes are divided into lobules of various sizes, their outline being most readily seen on the cut surface of foetal lungs ; they are separated by fine connective tissue. In structure they resemble a lung in miniature, having a terminal bronchiole, and a branch of the pulmonary artery and vein. 3. The Bronchi, on entering the lung, divide and redivide, each of the smaller divisions entering a lobule. In structure they resemble the trachea, with some, modifications. The cartilages in the larger tubes form more or less complete rings, but as the tubes get smaller the cartilages form in- complete rings, consist- ing of small plates in the walls arranged in a circular manner, and finally are wanting al- together in tubes of 1 mm. in diameter. The muscular fibres entirely surround the tubes, and may be traced into the finest ramifications. The elastic fibres extend to tubes of the smallest size, and become continuous with the elastic fibres forming the walls of the infun- dibula. The ciliated epithelium ceases before their entrance into the infundibula. 4. Terminal Bronchioles and Infundibula. After repeated sub-divisions, the bronchial tube, when reduced to 1 mm., is called a terminal, lobular, or respiratory bronchiole. Each terminal bronchiole ends in one or more enlarged passages called the alveolar passages or ducts, from which are given off blind Fir, 61. — Diagrammatic representation of a terminal bronchiole, alveolar pas- sages, and infundibula (Schafer). 128 Respiration dilatations, the infundibula or end-sacs. The walls of the terminal bronchioles are in part, and the walls of the alveolar passages and infundibula are completely, beset with a number of air-cells or alveoli, which open into them by wide apertures (fig. 61). In the terminal bronchioles the cartilages have disappeared, and the cylindrical ciliated epithelium is being replaced by a layer of small polyhedral granular cells, so that their walls consist of (a) a layer of granular cells ; (b) a muscular coat of non-striated fibres ; (c) a thin layer of elastic fibres, {a) The granular cells are gradually replaced by flat, trans- parent, nucleated cells, as the alveolar passages open into the infundibula ; these flattened cells also line the air-vesicles ; (b) the muscular coat is continued into the alveolar passages and infundibula, but does not surround the air-cells ; (c) the elastic fibres forming the outer coat of the terminal bronchioles are continued on to the air-cells, and form their walls (fig. 62). 5. Air-cells (fig 62) are about -25 mm. ( T ^„ in.) in diameter ; they are lined internally by flattened, transparent nucleated cells, continuous with those of the infundibula, and their walls are formed by the elastic fibres continuous with the elastic tissue of the bronchi and its divisions. According to Klein, the walls of the air-cells contain connective-tissue cells, the spaces which they occupy being continuous with the lymphatic capillaries. 6. Pulmonary Vessels and Lymphatics. — The pulmonary arteries accompany the bronchial tubes, and, like them, do not anastomose. Their terminal arterial branches, '025 mm. (-fuVo m -) m diameter, lie between the air-sacs, and send a net- work of capillaries over them. The bronchial arteries, two or three in number, arise from the aorta, are dis- tributed to the bronchi, lymphatic glands, connective Lungs 129 tissue, and mucous membrane. The right bronchial vein enters the vena-azygos, and the left the superior intercostal vein. The deep lymphatics arise from the Fig. 62.— Section of cat's lung, highly magnified, stained with nitrate of silver (Klein). In the centre is seen an infundibulum in cross section, lin. d with flattened epithelium, groups of polyhedral cells are seen on one side ; the alveoli are lined by flattened cells and here and there a polyhedral cell. lacunae or spaces occupied by the connective-tissue cells in the elastic tissue around the air-sacs. They K 1 30 Respiration empty themselves into the perivascular lymphatics. The superficial lymphatics enter the subpleural lym- phatics, and all eventually enter the bronchial lymph- glands. Pleurae. — The lungs are surrounded by a serous membrane which adheres to the pulmonary surface and is also reflected on to, and lines the inner surface of, the thoracic cavity. The pulmonary and costal pleural surfaces are always in contact under normal conditions, so that there is no pleural cavity, and the smooth moist surfaces prevent friction. When the pleura is inflamed the pleural surfaces become roughened by the exudation of lymph, a ' friction sound ' or ' rub ' is heard, being caused by the rough surfaces rubbing together. Sometimes serum is effused, and then the pleural and costal surfaces are separated from one another by the effused fluid (pleuritic effusion). Mechanism of Respiration The lungs are compound elastic bags communi- cating with the outside air by means of the bronchi and trachea and suspended in a semi-distended state in an air-tight cavity with movable walls. When the cavity of the thorax is enlarged by the contraction of certain muscles, the lungs become distended by draw- ing in air through the trachea. When the muscles relax, the lungs tend to collapse, expelling most of their contained air — a result due in part to the -con- traction of the elastic tissue they contain, and also to the recoil of the elastic rib cartilages. If an opening is made (as the result of accident or disease) either through the chest wall from outside or through the lung so that a bronchus communicates with a pleural cavity, the lung collapses, and air enters the pleural cavity {pneumo- thorax). If there is a Inspiration m wound on both sides, death immediately follows as neither lung can become inflated. Inspiration. — The chest enlarges in three direc- tions, viz., downwards, forwards, and laterally. The enlargement downwards is effected by the contraction of the diaphragm. At rest the diaphragm presents a convex surface to the thorax ; in contracting this sur- face becomes flatter, the floor of the chest is conse- quently lowered and the cavity of the thorax enlarged, and air enters to distend the lungs. The contraction of the diaphragm tends to press the abdominal viscera downwards and causes the walls of the abdomen to project during inspiration. The antero-lateral enlargement is effected by raising the ribs. A vertebro-sternal rib has two movable joints, the posterior where the head articu- lates with the sides of the bodies of the vertebne, and the anterior at the junction of the costal cartilage with the sternum. The anterior end occupies a lower position than the posterior, so that the rib is in more or less of an oblique position. When the anterior ends are raised the sternum will be pushed forward, and the antero-posterior diameter of the chest en- larged. In normal inspiration the antero-posterior diameter is increased 5 mill., in a deep inspiration about 30 mill. When the ribs are raised by the ex- ternal intercostal muscles, the angles which they make with the sternum become more obtuse, and the chest enlarged in the tranverse diameter. Muscles in action in easy inspiration : t. Diaphragm, enlarges the chest downwards. 2. Scaleni, fix the upper two ribs. 3. External intercostals J , , , ' 4. Levatores costarum 1 , ° ,, 1 3 2 Respiration Muscles which may be brought into action in laboured inspiration : i. Serratus posticus superior, raise 2nd, 3rd, 4th, and 5th ribs. 2. Sterno-mastoid, raise the clavicle. I raise the ribs, the scapula and arm being fixed. 3. Serratus magnus 4. Pedoralis major tres. Hydro-Carbons, or Fats. — These are neutral bodies derived from both animal and vegetable foods. They consist of olein, palmitin, and stearin. Olein and palmitin are met with both in animal and vegetable products ; olein is fluid at ordinary temperatures ; palmitin has a semi-fluid consistence. Stearin is a solid fat, is found only in animal products, and exists largely in suet. They all have glycerine for a base in combination with the corresponding fatty acids, oleic, palmitic, and stearic. The fats are remarkable for the small quantity of O they contain ; thus in palmitic acid C| , H 32 , O, the amount of O is about 12 per cent, of its weight, leaving from 80 to 90 per cent, available for force-production. Digestion of Fats. — The gastric juice dissolves the connective tissue binding together the fat -vesicles and sets free the fat. The fatty matters are emulsified in the small intestine by the action of the pancreatic juice, and in a lesser degree by the other secretions, and for the most part enter the lacteals, though a certain proportion, which has possibly become saponi- fied, enters the portal vein. Destiny of Fats. — The fats are utilised in the body for force- production, either immediately, or are stored as adipose tissue to be used when required. They therefore serve for the maintenance of heat and 1 54 Food performance of muscular work. The capacity of a material for force-production depends upon the amount of unoxidised C and H it contains, and of all alimen- tary substances fats take the highest place. Experi- mentally, Frankland has shown that the actual heat developed by the various alimentary substances when burnt in O is as follows : — i gramme beef fat . 9,069 gramme-degrees, butter . 7,264 „ „ beef muscle 5,103 ,, ,, ,, arrowroot . 3,912 „ „ That is to say, 1 gramme of fat, when burnt, will give off heat sufficient to raise 9,069 grammes of water i°C, whereas the same amount of arrowroot would, when burnt, only raise 3,912 grammes of water i°C. It is found that the inhabitants of arctic regions readily devour all kinds of fat, while in the tropics the foods of the inhabitants consist largely of farinaceous and saccharine matters. The force generated by the oxidation of the hydro-carbons is available for muscular work. A large amount of muscular work can be performed on a fatty or starchy diet. During muscular exercise the amount of C0 2 given off by the lungs varies according to the work done : thus, 5 grains per minute during sleep, and 25 grains per minute walking at the rate of three miles an hour. The amount of mechanical work obtainable from the oxidation of — 1 gramme beef fat = 3,841 kilogramme-metres, butter = 3,077 ,, beef muscle = 2,161 „ ,, „ arrowroot = 1,657 ,, „ that is, the force derivable from the oxidation of 1 gramme of fat is sufficient to raise 3,841 kilo- Starches , 155 grammes one metre. (1 kilogramme-metre = 7^32 foot-pounds.) The products of the combustion of fat are H 2 and C0 2 . Animal life cannot long be maintained on a non- nitrogenous diet. Dogs fed on fat, or fat and starches, emaciate and die. Nitrogenous food is re- quired to renew the tissues, which become wasted and worn during the processes of life. Carbo-Hydrates or Amyloids These comprise starch, cane sugar, grape sugar, milk sugar, glycogen. Chemically these bodies differ from the fats in containing a smaller quantity of uncombined carbon and hydrogen, the O existing in sufficient quantity to form water with all the H present, as in starch (C H| O.-,), grape sugar (C H, 2 O fi ). Starch is met with in vegetable products. It is prepared for absorption by being converted into grape sugar in the mouth and small intestine. Cane sugar and glycogen are converted into grape sugar in the stomach and intestines. Milk sugar (C] 2 H. 22 M + H 2 0) and grape sugar (C 6 H l2 6 + H 2 0) are readily absorbed by the portal vein and submitted to the action of the liver. Here some change takes place. Sugar injected into the jugular vein rapidly appears in the urine ; injected into the portal, it does not, unless in large quantity. The grape sugar is con- verted in the liver into glycogen (C (i H l2 6 - H 2 0— - C (i H, O s ), and probably also into fat. It is uncertain whether the glycogen is reconverted into sugar and oxidised in the system, or whether it enters the system as glycogen or some similar body. In any case it is oxidised, being converted into C0 2 and H 2 0, and giving rise to heat, and supplying force for the performance of work. 156 Food INORGANIC MATERIALS Various salts exist in the body in combination with the organic materials that form the tissues. The chief salts consist of calcium, sodium, potassium, magnesium, and iron, in combination with chlorine, and phosphoric, carbonic, and sulphuric acids. The various salts form an essential part of the food, inasmuch as they exist in every tissue of the body. They exist in most forms of food consumed, both animal and vegetable, in milk, in drinking- water. Water is an important element in the food ; it forms nearly 60 per cent, of the body-weight, and is constantly being lost to the body through the lungs, kidneys, and skin. DIETETICS Experience proves that a mixed diet is the best to maintain the body in health. Dogs will not live on hydro-carbons or carbo-hydrates alone. Too much nitrogenous food leads to an excessive amount of urea and uric acid, and throws increased work on the excretory organs. Milk, the food of early life, may be taken as a typical illustration of a natural combination of the various foods. Milk contains — Water .... Solids .... Nitrogenous matters (casein- Woman 87-88 12-13 Cow 86-87 ogen and albumen) . Milk sugar , . . . Fat . " . Ash 1 7 4- -2 ■5 "2 4 4" 4- -s -5 '7 Cow's milk differs from woman's milk in that it contains a larger amount of caseinogen, it curdles more quickly, and the precipitated casein is heavier Dietetics 1 5 7 and more solid than the casein of woman's milk. There is less sugar in cow's milk than woman's milk. In comparing cow's milk with the diet of an adult given below, it will be noted that starches take the place of some of the fat. If cow's milk were substi- tuted for the diet given for moderate work, some eight pints would have to be taken daily. The amount of water taken would be an objection. Milk is, however, an easily digested food, and is much used as a food in illness. Diet for Moderate Work. — The normal diet for a man in health can only be arrived at by experi- ence. Taking the average of a large number of healthy persons, it has been found that the following diet will suffice : — Albuminous matter . ozs. avoir. • 4'5 gramme I30 Fatty matter Carbo-hydrates Salts • 3'° I4'2 I'O 3 4 404 3° Thus about 23 ozs. of dry solid food are contained in this standard diet, about ith of which is nitrogenous. If we reckon that 50 per cent, of ordinary food is water, these 23 ozs. will correspond to 46 ozs. of ordinary solid food. In addition about 50-80 ozs. of water are taken. The force -producing value of this standard diet is nearly 4,000 foot-tons. The standard diet will necessarily be altered under different conditions. It is said that an Esquimaux eats about 20 lbs. of flesh and oil daily, and men working heavily necessarily require more than when at rest. Diet for Idleness. — ozs. • grammes Albuminous matter . . . 2-5 77 Fats i'o 28 Carbo-hydrates. . . . 12-0 340 Salts 0-5 14 158 Food This diet will keep a man alive, but is not sufficient if he performs any work. Hard Work Diet. — The average dietary of a labourer performing hard work has been calculated at— Nitrogenous matter . ozs. 5'° grammes 142 Fat Carbo-hydrates . Mineral .... 3'° 22'2 I'O 84 630 3° Dynamic value, 5,232 foot-tons. Taking the model diet for ordinary men N. c. OZS. grains grains Nitrogenous matter . • 4'S 316 I,o68 Fat . • 3'° 1,024 Carbo-hydrates • 14*25 2,768 316 4,860 It appears, therefore, that a man on ordinary diet and doing an ordinary amount of work requires 300 grains of N and 4,800 grains of C. (N 20 grammes and C 320 grammes.) The ratio of the quantities is 1 : 15. In albumen the ratio is 1 : 3'5. Hence, if albumen alone were used, and the 300 grains of N were supplied, there would be a deficiency of C, and if the 4,800 grains of C were supplied, there would be more N than required. In bread the ratio is as 1 : 30 ; so that if bread alone were used there would be a superfluity or deficiency of either N or C. Two pounds of bread and three-quarters of a pound of meat will fulfil the above conditions, though they will do so better if 1-2 ozs. of butter be added. Or the following more varied diet may be adopted : — Dietaries 159 1 1 lb. bread Foundation j i lb. meat 1 1 lb. fat . 1 1 lb. potatoes . ■ U pint milk Accessories -\ f ii I t lb - eggs . -L lb. cheese c grammes 117 34 84 45 20 J5 20 335 N grammes 5'5 7'5 ''3 2 3 21 Table showing amount of different constituents in some foods In 100 parts Articles Water Albu- minates Fats Carbo- hydrates Salts Beef and mutton . 75 IS 8-4 1-6 Bacon . 15 8-8 73'3 — 2-9 Salt beef 49-1 29-6 •2 — 211 White fish 78 i8-i 2-9 — 1 Flour . IS 11 2 70-3 17 Bread . 40 8 i-5 49 '2 i - 3 Rice 10 5 •8 83-2 •5 Oatmeal IS 126 5-6 63 3 Peas (dry) is 22 2 S3 2-4 Potatoes 74 i-5 ■1 23-4 1 Eggs • 73"5 i3'5 n-6 — 1 • Cheese . 36-8 33'5 24-3 — 5'4 Milk . 867 4 37 5 •6 Butter . 6 •3 9i 27 Sugar . 3 96'S •5 (After Parkes) Dietaries. — Infancy. — The newly-born infant is generally put to the breast a few hours after birth, but a few days generally elapse before the flow of the breast-milk is thoroughly established, especially in the 1 60 Food case of women who are mothers for the first time. The infant at first takes the breast every two hours during the day, and every four to six hours during the night for the first six weeks or two months. After this the infant takes more at a meal, and only takes the breast every four hours during the day, and sleeps most of the night. The infant is to be nursed entirely from the breast for the first six or seven months. If artificially fed for the first month, it should take 10 ozs. of good cow's milk, diluted with an equal quantity of barley-water or freshly-made whey ; 2 ozs. being given every two or three hours during the day, and every four hours at night. This amount should be gradually increased so that at three months of age 20 ozs. of milk diluted with 10 ozs. of barley-water or whey may be given every four or five hours. This amount should be again increased so that when the infant is six months of age it is taking 30 ozs. of undiluted milk. From six months to twelve months. — From 30 ozs. to 40 ozs. of milk should be taken, the milk being thickened at some of the meals with some starchy form of food, as rusks, biscuit powder, &c. As the salivary and pancreatic secretions are not thoroughly established before the age of six months, it is unwise to give children any starchy foods before that date. From one to two years. — At a year old, thicker food, taken with a spoon instead of through a bottle, may be given. First meal, 7.30 a.m. — 8 to 10 ozs. of milk, with finely crumbled bread or porridge. Second meal, 11 a.m. — 6 to 8 ozs. of milk and water. Third meal, 1.30 p.m. — Some gravy or beef-tea, with sopped bread-crumbs ; or a tablespoonful of Diets 161 finely minced mutton chop and bread-crumbs. Light pudding. Fourth meal, 6 p.m. — Same as th,e first. Diet for Children : Breakfast. — Bread and butter, or porridge, 5 ozs. ; milk, 10 ozs. or ad lib., may be diluted with weak tea and sugar added. Dinner. — Cooked meat, 3 ozs. ; vegetables. 2 ozs. ; light pudding, 4 ozs. Tea. — Bread and butter, 5 ozs, ; milk, 10 ozs. or ad lib. Diet for Adults: — {Full Hospital Diet) — Breakfast. — Bread, 10 ozs. ; butter, § oz. ; coffee or tea, with milk and sugar, 15 ozs. Dinner. — Cooked meat, 6 ozs. ; potatoes, 8 ozs. ; light pudding, 8 ozs. Tea. — Bread, 10 ozs. ; butter, \ oz. ; coffee or tea, 15 ozs. Supper. — Milk or bread and milk, 10 ozs. An examination of the gains and losses of an adult man in twenty-four hours, as estimated by direct experiment when the body-weight was remaining nearly stationary, showed that the gains in the form of food, &c, roughly amounted to 20 grammes of N, 315 grammes of C, 2,000 grammes of water, and 24 grammes of salts ; 709 grammes of O in respiration ; these being equal to about v.'nth the weight of the body. His losses by the urine, feces, and expired products amounted to 20 grammes of N, 274 of C, 248 of H, mostly in form of water, 2,630 of O in the form of C0 2 or H 2 and 24 of salts ; leaving him 145 grammes heavier than before. Effects of an Insufficient or Improper Diet. — Infants or adults supplied with insufficient M 1 62 Food food become pale, emaciated and weak, and often die of some intercurrent disease, to which their weak state predisposes, as diarrhoea or dysentery. The effects of an improper diet are especially seen in infants when fed on thick starchy foods. They suffer from indigestion, flatulence, constipation or diarrhoea, are fretful and sleepless, become wasted, suffer from skin eruptions, and are frequently convulsed before death. Effects of Food in Excess— One of the principal effects of an excess of proteid food is to throw extra work on the excretory organs, as • the liver and kidneys : this result is especially marked when little exercise is taken. Gout, lithaemia with disordered liver and kidneys, and indigestion are apt to follow. The effects of an excessive amount of fatty or starchy matters in the food is sometimes shown in the excessive formation of adipose tissue, and often in indigestion. Effects of Starvation. — The most prominent symptoms are, first, pain in the epigastrium, relieved by pressure ; this subsides in a day or two, and is succeeded by a feeling of weakness and of intense thirst. The countenance becomes pale, the body exhales a peculiar fcetor, and the bodily strength rapidly fails. The temperature is lower than normal. The mental powers exhibit similar weakness, first stupidity, then imbecility, which sometimes is suc- ceeded by maniacal delirium. Life terminates by gradually increasing torpidity, or, occasionally, by a convulsive paroxysm. With entire abstinence from food and drink, death occurs in from eight to ten days. The Welsh fasting girl lived eight days. This time may be prolonged if water can be obtained, or if surrounded by a warm damp medium. The loss during starvation falls most heavily on Digestion 163 the fat, next the glandular organs, then the muscles, the heart and brain being affected least The post- mortem examination shows extreme emaciation and complete absence of fat. All the organs, with per- haps the exception of the brain, are bloodless ; the coats of the intestines are thin and empty of contents ; the gall-bladder full, the bile staining the surrounding parts. The body rapidly passes into decomposition. The percentage of dry solid matter lost during thirteen days by the most important tissues of a cat was as follows :— Adipose tissue 97 "o per cent Spleen 63'i V Liver . 56-6 „ Muscles 3°' 2 » Blood 17-6 „ Brain and cord o'o „ CHAPTER XIII DIGI iSl 'ION Teeth. Two sets of teeth make their appearance during the life of man :— I. The temporary or milk teeth (20). II. The permanent set (32). I. The Temporary Set appear during the first two years of life. They consist of two incisors, one canine, and two molars in each half-jaw, making twenty in all. They make their appearance through the gums in M 2 164 Teeth five groups, in the following order (though exceptions occur even in healthy children) : — First group — Two lower central incisors .... 6th- 8th months. Second group — Four upper in- cisors ..... 8th-ioth „ Third group — Two lateral lower incisors and first four molars . i2th-i4th ,, Fourth group — Four canines . i8th-2oth „ Fifth group — Four back molars . 2oth~3oth „ II. The Permanent Set. — The first six months of life arc passed without any teeth ; by the end of the second year or middle of the third the milk teeth have all appeared, and these begin to be replaced by the permanent set at the sixth year, and are completely replaced by them at the twelfth or thirteenth year ; the teeth being completed by the eruption of the wisdom teeth at the age of about twenty-one. When com- plete there are thirty-two, there being two incisors, one canine, two bicuspids, and and three molars, in the half of either jaw. The molars of the temporary set are replaced by the permanent bicuspids ; the three permanent molars appear in the jaw behind the molars of the milk teeth. The permanent teeth appear in the following order : — 6th year First molars. 7th „ Two central incisors 8th ., Two lateral incisors. 9th „ First bicuspids. 10th ,,. Second bicuspids. nth-i2th years Canines. i2th-i3th ,, . Second molars. i7th-2ist ,, Wisdom teeth. The Incisors (8) are arranged side by side in the Structure of the Teeth 165 front of the jaws. They have a single long conical fang, and a sharp chisel-shaped edge, for dividing the food. The Canines (4) are placed singly next to the lateral incisors. Their fangs are single, large, and conical, compressed laterally, and cause a prominent ridge on the alveolus of the jaw. The crown is more pointed than in the incisors. The Bicuspids (8) are arranged four in each jaw. The fangs are bifid at their apices, more marked in the upper and second bicuspids, and are grooved laterally. The crown is compressed from before back- wards, and is surmounted by two tubercles, or cusps, separated by a groove. The Molars (12) are arranged three in each jaw, behind the bicuspids. They have from two to three fangs. In the two anterior molars of the upper jaw there are three in number, two external and one internal. The two anterior molars of the lower jaw- have two fangs, one anterior and one posterior. In the third molar, or wisdom tooth, the fang is irregular and single. The crowns of the molar teeth are cuboidal in form, rounded on each lateral surface, and flattened in front and behind. The upper molars have four cusps at the angles of the grinding surface, separated by a crucial depression ; the lower molars have five cusps. The molars, from the great breadth of their crowns, are suitable for grinding and pound- ing the food. Structure. — Minute anatomy : A tooth consists of a croiifn, which projects from the gum ; a root, or fangs, which are fixed in a socket in the bone ; and a short intermediate ?ieck. Each is supplied with an artery and nerve, and has a central cavity filled with a soft vascular sensitive substance, the pulp. On ver- tical section a tooth shows — 1. Pulp. 3. Dentine. 2. Crusta petrosa. 4. Enamel. 1 66 Teeth i. The Pulp .occupies the central cavity of the tooth, and consists of fine connective tissue, nucle- ated cells, blood-vessels, and nerves, The cells, or Fig. 67.— Vertical section of the upper part of an incisor tooth (IColliker). «, the pulp cavity ; £, dentine ; c, arched incremental fibres ; d, cement : c, enamel with bands indicating the direction of the range of fibres ',/, coloured lines of the enamel. odontoblasts, form a stratum on the surface of the pulp, and send fine processes into the dentine tubules. The arteries are derived from the internal maxillary, and the nerves from the fifth pair. Development of the Teeth 167 2. The Crusta petrosa, or cement, covers the fang of the tooth, its place being taken below by the enamel which covers the crown. In structure it re- sembles bone, containing lacunas and canaliculi, but they are larger and more irregular. 3. The Dentine forms the principal mass of the teeth ; it is protected by the crusta petrosa and enamel, and hollowed out in the centre to form the pulp- cavity. It is somewhat harder than bone, and differs from it in structure. It is penetrated by numerous fine tubes, giving it a striated appearance beneath the microscope, the tubes appearing dark and the matrix in which they lie transparent. The tubules open into the pulp-cavity, and radiate to the periphery, giving off small branches. They are 5 ,-,'„„■ in. in breadth, and have a distinct wall, the dental sheath. As the dentine is sensitive, it is possible they may convey nerve-fibres as well as prolongations' of the cells of the pulp-cavity. The matrix is homogeneous. 4. The Enamel is very hard and covers the crown. It is made up of microscopic prisms arranged side by side ; these prisms are six-sided and -50V0 m> m diameter, and are marked at intervals by transverse lines. Chemical Composition. — The hard tissues of the teeth, like bone, consist of animal and mineral matter : the former yields gelatin on boiling, and exists in different amounts in the tissues — Bone . . 33 per cent, animal matter. Crusta petrosa 30 ,, „ ,, Dentine . . 28 ,, ,, ,, Enamel . . 3-5 „ „ „ The mineral matter consists of calcic phosphate and carbonate, magnesic phosphate and calcic fluo- ride. Development.- At the seventh week of intra- i68 Teeth uterine life a groove appears on the surface of the jaws, which involves the soft embryonic tissues of the jaw as well as the Malpighian layer of the epithelium. It was called by Goodsir the primitive dental groove. This down-growth of epithelium forms the common enamel-germ (fig. 68, /), and from it the enamel is developed. From the bottom of this groove, which has become flask-shaped in section, papilla;, ten in number, arise. These papillae, as they grow up- wards, push before them and become surrounded by the enamel-germ, and the portion of the primi- tive groove in which each is situated becomes cut off from the rest, so that now the papilla has be- come enclosed in a cavity. In the meantime the cavity containing the papilla becomes sur- rounded by a vascular membrane, the dental sac. The papilla now acquires more and more the shape of the future tooth (fig. 69/). By the end of the fourth month of foetal life, thin caps of dentine are formed on the papillae of all the temporary set. At the time of birth, the crowns of the anterior milk teeth are fully formed (see fig. 70). After birth de- velopment proceeds, the fangs being formed, so that they are ready to be cut in the order given (p. 164). The sacs in which the ten permanent teeth which replace the temporary are formed are derived from the neck of the enamel-germ (fig. 69 B,/p), and these form the enamel-germs of the future teeth. The pos- terior permanent teeth (the three molars) arise from Fig. 68.— Section across the upper jaw of a fecial sheep (Waldeyer). c c', epithelium of mouth ; j\ neck ; _/"', body of special enamel genu. Development of the Teeth 169 Fig. 69.— Sections at later stages than fig. 68, the papilla having become formed (KLolliker). c, epithelium of the gum ;/ t neck of enamel germ ; f\ enamel organ ; e, its deeper columnar cells ; p, papilla ; s, dental sac ; B,y^*, enamel germ of permanent tooth. 170 Digestion sacs formed by an extension backwards of the original groove. At birth only one of the permanent set, namely, the first molar, has its cap of dentine already formed though this is not constant ; the calcification of the incisors commences a month or so after birth, the "ig. 70. — The dental sac exposed in the jaw of a child at birth (Quain's Anatomy). ) sympathetic from the cervical sympathetic. Deglutition Deglutition is a complicated act by means of which food passes from the mouth into the oesophagus without any part of it being allowed to enter the nasal cavity or the larynx. It is usually divided into three acts : — i. The passage of food to the back of the mouth. 2. Its passage across the orifice of the larynx. 3. Its seizure by the constrictors and its passage through the oesophagus to the stomach. 1. The bolus having been prepared, the tongue carries it back through the anterior pillars of the fauces, the movement being effected through the agency of the stylo-glossus and intrinsic muscles of the tongue. 2. The soft palate is raised by the action of its muscles, and, assisted by the contraction of the upper part of the superior constrictor, shuts off the cavity of (Esophagus 1 8 1 the nose from the pharynx. The larynx is raised behind the hyoid bone by the action of the stylo- pharyngeus and thyro-hyoid, the vocal chords are approximated, and the epiglottis closely fitted over the rima glottidis by the action of the depressor. The passages both into the nares and larynx being closed, the descending bolus passes over the root of tongue, the epiglottis, and beneath a roof formed by the contraction and approximation of the palato- pharyngeal muscle, is seized by the constrictors, and propelled into the cesophagus. The first stage in which the tongue passes the bolus to the isthmus faucium is a voluntary act. The second and third are purely reflex, and can take place independently of the will and during sleep, or in a state of coma. Patients who are unconscious will swallow if partly raised up and liquids out of a ' feeder ' be allowed slowly to trickle over the back of the tongue. In profound coma swallowing becomes impossible. The respiration centre is lower in the medulla than the deglutition centre, and may continue in action when the former will not. Deglutition is a reflex act. The afferent nerves are the glosso-pharyngeal and branches of the 5th. The nerve centre is in the medulla. The efferent nerves are the pharyngeal branch of vagus, hypo-glossal, glosso- pharyngeal, and facial. The (Esophagus The cesophagus is the muscular tube extending from the pharynx to the stomach. It consists of three coats : — 1. External, or muscular. 2. Middle, or submucous. 3. Internal, or mucous. 1 82 Digestion i. The External consists of an outer layer of longitudinal and an inner layer of circular muscular fibres. The muscular fibres in the upper part are striated, but are gradually replaced by non-striated in the lower half. 2. The Submucous coat consists of connective tissue, and contains some mucous glands. 3. The Mucous coat is pale in colour, and when the oesophagus is contracted is thrown into longitu- dinal folds. In structure it resembles the skin, having a cutis, papilte, rete mucosum, lined by stratified flattened cells. The food is propelled along the oesophagus by the peristaltic action of its muscular walls. It is a reflex act ; the afferent and efferent nerves are supplied by the vagus. The centre is in the medulla. Food ac- cumulates in the oesophagus of an animal in which the vagus is divided below the pharyngeal branches, the animal being able to swallow the food, but the oesophagus fails to pass it on into the stomach, from paralysis of its muscular walls. Stomach The stomach is a somewhat conical or pyriform- shaped sac, the left extremity or cardiac end being the larger, the right or pyloric extremity being the smaller. It has two orifices, the cardiac, where the oesophagus enters, and the pyloric orifice, at the entrance into the duodenum. When moderately distended it measures 10 to 12 inches in length, and from 4 to 5 inches in breadth. Structure. — Four coats : 1. Serous. 3. Submucous. 2. Muscular. 4. Mucous. 1. The Serous is divided from the peritoneum ; it invests the whole organ, except at the curvatures. Stomach 183 2. The Muscular contains fibres of the non- striated variety. Longitudinal , best marked along the curvatures and near the pylorus. Circular, forming a complete layer over the whole extent of stomach, becoming thick and strong at the pylorus, and form- ing the sphincter. Oblique, scattered over surface and continuous with circular of cesophagus. 3. The Submucous consists of a layer of con- nective tissue between the muscular and mucous. 4. The Mucous is a smooth pink membrane, which is loosely attached to the tissue beneath, and when the stomach is empty is thrown into ruga;. The mucous membrane contains a fine layer of muscular tissue, the muscularis mucosa, internal to which are the tubular glands. The epithelium of the surface consists of a layer of columnar cells extending into the mouths or ducts of the gastric glands. Gastric Glands. — On examining a section of stomach stained with logwood or aniline, rendered transparent with glycerine, the gastric glands are seen parallel to one another and closely crowded together with their blind extremities towards the muscularis mucosa?, and opening on the surface of the mucous membrane. Their length varies from -£$ to -^ in., and diameter ^^ to -g^ v in. in breadth. Two different kinds of glands are distinguished ; some in larger numbers near the pyloric orifice are lined throughout with columnar epithelium, and are supposed to secrete mucus, and are called pyloric glands. The cardiac glands, so named from the portion of the stomach where they occur most numerously, have columnar epithelium at their mouths only, the rest of the gland being lined by two different sets of cells ; those at the circumference of the tubule resting on the base- ment membrane, being oval in shape, are called ovoid ox parietal, and another set, occupying a more central 1 84 Digestion position, are cubical or columnar in shape, and are termed central cells. These cells are finely granular, and contain an oval nucleus. They are occupied by num- erous granules during fast- ing, but which, like the granules in the cells of the salivary glands, mostly dis- appear during a period of activity. Delicate lymphoid tissue may be seen in places in the mucous membrane, resembling the solitary glands of the intestine. The Gastric Juice is a thin colourless acid fluid of specific gravity iooi to ioio, containing | to i per cent, of solids in man. The daily amount varies, an average being 10 to 12 pints (7 to 8 litres). It contains — 1. Pepsin. 2. A curdling ferment. 3. Hydrochloric acid (free). 4. Mucin. 5. Salts and water. About two-thirds of the solid matter consists of peptones and pepsin, and one-third of salts. Amount of free HC1='2 per cent. Artificial Gastric Juice is best prepared by dissecting off the mucous membrane of the stomach Fie. 75. — A cardiac gland from the bat's slomach (Langley). c, columnar epithelium of the surface ; n, neck of the gland with central and parietal cells ; /, base or fundus, occupied only hy principal or central cells, which exhibit the granules ac- cumulated near the lumen of the gland. Gastric Juice 185 of a pig, cutting it into small pieces, and digesting it in glycerine for a few days, filtering and adding fresh mucous membrane. This may be repeated several times. Each time the glycerine will take up a fresh quantity. A little of the glycerine extract added to •2 per cent, solution of HC1 will form an active arti- ficial-gastric juice. Action of Gastric Juice on Proteids.— The characteristic action of gastric juice is its action on albuminous compounds, converting them into the peptones. If shreds of fibrin be placed in artificial gastric juice and kept at a temperature of 38 C, the shreds first swell up and become transparent, then gradually dissolve, leaving only some slight fiocculent remains. If thin slices of white of egg be similarly treated, the edges become translucent, and finally they completely disappear. This completes the first stage of the process, the proteid being converted into acid-albumen or syntonin. As the digestion proceeds, first albuminose and finally peptone is formed. Both albuminose and peptone are present in the final pro- duct. The presence of these three bodies can be de- monstrated in the following way : — (1) by neutralising a portion of the fluid with sodium carbonate, a pre- cipitate of acid-albumen occurs ; (2) on adding HN0 3 to another portion, a precipitate of albuminose occurs, which disappears on warming and reappears on cooling; (3) a third portion of the fluid is shaken with amnion, sulphate, filtered, and the filtrate tested for peptone by adding NaHO and a trace of copper sulphate, a rose colour indicates that peptone is present (see p. 18). Action on various Foods. — Cooking renders meat more digestible by separating and breaking down the fibres. When exposed to the action of gastric juice the connective tissue is dissolved, and the fibres set free ; the transverse striae become well-marked, the fibres show a tendency to transverse cleavage, and 1 86 Digestion finally become broken up and disappear. The fatty matters are set free from their envelopes. Fish and eggs are digested in the stomach in about one hour and a half ; beef, mutton, and fowls in two and a half to three hours. The gluten of bread is dissolved and converted, like albumen, into peptone, the starch being set free. Milk is quickly coagulated by gastric juice, the casein being precipitated ; this is apparently brought about by the action of a curdling ferment. The coagulated casein is quickly redissolved and con- verted into peptone. Gastric juice has no action upon elastic tissue, cellulose, starch, or mucus. The part that pepsin plays in digestion is that of a ferment resembling the action of ptyalin in the saliva. Pepsin is not destroyed in the act of diges- tion ; its digestive power appears to be infinite. Yet, if more and more fibrin be added to artificial gastric juice, it will at last remain undissolved, the arrest of digestion being due to an accumulation of the pep- tones and want of acid. For, if the liquid be diluted and more acid added, digestion will recommence. The activity of the pepsin is greatest at a temperature of 3o°-so° C. (9o°-ii2° F.). It is completely de- stroyed by boiling. Gastric juice contains a distinct ferment which has the property of curdling milk (W. Roberts). Chyme. — The grumous acid fluid, resulting from the digestion of the food in the stomach, is termed chyme. It contains (i) peptones resulting from the conversion of various proteid substances — albumen, fibrin, casein, gelatin, &c. ; (2) various partly-digested proteids, as muscular fibre, connective tissue ; (3) cer- tain substances which are not digested in the stomach, as fat, cellulose, elastic tissue, starch, &c. ; (4) various salts and sugar in solution. Digestion of the Stomach. — If a quantity of Stomach 187 milk be introduced into the stomach of a rabbit and the animal killed an hour after and laid in a warm place for twenty four hours, the walls of the stomach I it 111 ! ■■; ^ , ■ / - " . -' ■■ %dlar Frc 76. — Diagram of the abdominal part of the alimentary canal (Brinton). c, the cardiac ; p, pyloric end of the stomach ; d, the duodenum ; J, 1, convolutions of the small intestine ; cc, csecum ; AC, ascending colon ; tc, transverse, and nc, descending colon ;sf, sigmoid flexure ; r, rectum ; A, anus, will probably be found digested. If a portion of the stomach of a dog be ligatured, the wounded stomach 188 Digestion <•>' sewn up, and the dog allowed to live a few hours, the portion included in the ligature will be digested. The stomach itself is not digested during life, in conse- quence of the circulation through its walls of alkaline blood. Secretion of Gastric Juice. — The stomach has two secretions, one thick, tenacious, and alkaline — the gastric mucus ; the other, thin, acid, and watery — the gastric juice proper. The former is secreted during fasting, while the latter is only secreted when food or fluid enters the stomach. Saliva or alkalies, pepper, alcohol, excite the secretion of gastric juice. Their action is reflex : the vagus is probably the afferent nerve, which acting on the medulla, inhibits the sympathetic and dilates the blood-vessels supply- ing the glands ; the efferent impulses descending along the splanchnics. Movements of the Stomach.— Food during digestion in the stomach is kept in motion by the peristaltic action of its walls. By the contraction of its muscular fibres currents are set up in its contents, the food travelling along the large curvature and re- turning by the lesser, while as digestion proceeds certain portions are passed through the pylorus into the duodenum. Vomiting is a reflex act by which the contents of the stomach are expelled through the oesophagus and mouth. Very different circumstances may give rise to vomiting : — i. Irritation of the terminal fibres of the vagus from the presence in the stomach of certain sub- stances, as ipecacuanha, or a catarrhal state of the mucous membrane. 2. Irritation of the terminal fibres of different branches of the vagus or sympathetic, as in tickling the fauces, an inflamed peritoneum, an enlargement of the uterus, as in the vomiting of pregnancy. Small Intestine 189 » 3. Direct irritation of the nervous centres, as in tumour of the brain, or circulation through the nerve- centres of certain substances, as apomorphia. 4. Vomiting may also be induced by disgusting smells, sights, or tastes. The afferent nerves depend upon the cause ; they may be the vagus, sympathetic, first, second, &c. The nerve centre is probably in the medulla. The efferent nerves are the phrenics and nerves to the abdominal muscles. Mechanism of Vomiting.— Peristaltic waves run from the pylorus to the cardiac end of the stomach, the cardiac aperture being firmly closed. Then, a deep breath having been taken, the diaphragm fixed, and glottis closed, the cardiac sphincter is sud- denly opened by fibres continuous with the longitu- dinal fibres of the oesophagus, the abdominal muscles contract, and, the stomach being fixed by the dia phragm, its contents are expelled. Structure of Small Intestine. — The small intestine commences at the pylorus and empties itself into the caecum, and is about 20 ft. in length. It is divided into three portions, the duodenum, occupying the first 10 or 12 inches, the upper two-fifths of the remainder being jejunum, and the lower three-fifths ileum. It has four coats, serous, muscular, submucous, mucous. The Serous entirely surrounds the gut, except where the vessels enter. The Muscular con- sists of two layers, external longitudinal and internal circular. The Submucous is a loose connective tissue layer between the mucous and muscular. The Mucous lines the intestine, and in the upper part of the jejunum is thrown into numerous transverse folds called the valvules conniventes, which are permanent and extend about two-thirds of the way round the in- testine. They increase the absorbing surface and help to delay the contents of the intestine. The mucous 190 Digestion coat is separated from the areolar by a thin layer of muscular fibres, the muscularis mucosa, and like the stomach, is lined by columnar epithelium. It is pro- vided with — i. Villi. 2. Brunner's glands. 3. Crypts of Lieberkuhn. 4. Solitary glands. 5. Peyer's glands. 6. Lymphoid tissue and vessels. 1. The Villi are small processes of mucous mem- brane which extend from the pylorus to the ileo-csecal Fig. 77. — Transverse section of a villus of the cat's intestine (Schafer). c, columnar epithelium ; g, goblet cell ; /, lymph corpuscles between epithelium ; 6, basement membrane ; c, blood capillaries ; ;«, section of muscular fibre ; ci, central lacteal. valve, and give the inner surface of the intestine a velvety appearance. They are about ¥ ] „ to J a inch in length, and are closely set together. They consist of an external layer of columnar epithelium, a basement membrane, a plexus of capillary vessels, a lacteal Small Intestin ne 191 vessel, a few muscular fibre cells prolonged from the muscularis mucosae, and lymphoid tissue-(fig. 77). 2. Brunner's Glands are small compound glands existing in the duodenum. They consist of clusters of acini in connection with a minute duct, which opens on the surface. 3. The Crypts of Lieberkuhn are minute blind tubes which exist in every part of the intestine Fig. 78.— Vertical section of a portion of a patch of Peyer's glands, with lacteal vessels injected (Frey). a, villi ; 6, tubular glands ; c, muscular layer of the'mucous membrane ; it, cupola or projecting part of a lymphoid gland ; e, their central part ; /", g, g', lacteal vessels. opening between the villi. They are lined by co- lumnar epithelium, and are T \- s inch to 3-^ inch in length (fig. 78 b). 4. The Solitary Glands are small white bodies about the size of millet seeds scattered through the 192 Digestion intestine. They consist of lymphoid tissue surrounded by a plexus of capillaries. 5. Peyer's Glands are a group of glands re- sembling the solitary glands in structure (fig 78). They are situated for the most part in the lower portion of the ileum. The groups are oblong, and placed lengthways in the intestine opposite to the attachment of the mesentery. 6. Lymphoid tissue is found in various places in the submucous tissue, in addition to that of the solitary and Peyer's glands. Secretions poured into the Intestine Bile is an alkaline, golden-yellow fluid of a bitter taste and specific gravity 10 18, and containing about 14 per cent, solid matter. If it remain long in the gall-bladder it becomes viscid, from the presence of mucus. From 30 to 40 ozs., or 1000-1500 grammes, are secreted in 24 hours. Composition : — Per cent. I. Mucin | 2. Bile-pigments . . J 2 ~3 3* Sodium salts of bile-acids . 9-10 4- Cholesterin . . 1 5- Lecithin 1 ' 2_- 3 6. Salts ■5-i 7- Water .... 85-86 Bile-pigments. — The yellow colour of the bile ot man and the carnivora is due to Bilirubin ; the green colour of herbivora and that of man after oxidation is due to Biliverdin. A small quantity of Biliprasin may be present. Gmeliris Test.— When strong yellow nitric acid is added to bilirubin or human bile on a white plate, Bile 193 a succession of colours is produced in the order of the colours of the spectrum — green, blue, violet, indigo, and red. If biliverdin be used the same result occurs, the first colour being blue. In apply- ing the test to urine, care must be taken to notice the colours succeed one another in their right order, as the presence of indican may cause green, blue, and yellow colours. Bilirubin may be prepared from dog's bile by acidulating with acetic acid and shaking with chloro- form ; the chloroform dissolves the bilirubin, and on evaporation leaves the pigment of a red colour. Biliverdin may be obtained by allowing an alkaline solution of bile to become green by exposure to the air. The bilirubin is oxidised and biliverdin formed ; it may be separated by precipitating by HC1, dissolving in alcohol and evaporating. Bili- rubin is believed to be derived from haemoglobin during its passage through the liver. It seems to be identical with the haematoidin found in old blood- clots. The Bile-acids are taurocholic and glycocholic acids. These acids are composed of cholic acid in combination with taurin and glycocine. Pettenkofer 's Test. — A small quantity of dilute bile is mixed with a few drops of sugar (cane sugar) and strong H a S0 4 added ; the solution becomes first cherry red, then of a purple colour. Some other organic substances give a similar colour, but may be distinguished by the spectroscope. Preparation. — Bile is rubbed up with animal char- coal and dried at steam heat ; it is thus rendered colourless. The bile-acids are then dissolved out by absolute alcohol and precipitated by ether, as silky needles which readily take up moisture and form a syrupy fluid. Cholesterin can be obtained best from gall-stones 1 94 Digestion by boiling with alcohol and filtering while warm ; white rhombic crystals of cholesterin form (fig. 6). Uses of Bile i. Slight action in converting starch into sugar 2. Assists in emulsifying and saponifying fats. 3. Assists in the absorption of fats. 4. Increases peristaltic action. 5. Prevents putrefactive changes in intestines. The action that bile exerts in converting starch into sugar and in emulsifying fat is slight. Mucous membrane wetted with bile allows minute globules of fat to pass readily through it, and in this way it aids the absorption of fat. It increases the peri- staltic action of the intestine, thus aiding in the propulsion forwards of the contents of the intestine. It stimulates the contraction of muscular fibres of the villi, emptying the lacteal, and forcing onwards its contents. It checks putrefactive changes. In jaundice, where the bile is prevented from flowing into the intestine, there is a tendency to constipation and flatulence. Bile is being constantly secreted, and accumulates till required in the gall-bladder. When the acid con- tents of the' stomach enter the duodenum a reflex action is set up, leading to the contraction of the gall- bladder, and pouring out of bile into the intestine. Pancreas The pancreas is an elongated lobulated gland, which lies across the abdomen, behind the stomach, and in front of the first lumbar vertebra. It is 6 to 8 inches long, 1 !, inches average thickness, and weighs from -2.\ to 3?? ounces. Pancreas 195 The cells lining the alveoli are columnar or pyra- midal in shape, with a spherical nucleus. Their protoplasm is finely granular near the lumen, and transparent near the basement membrane (fig. 79). After a period of activity, nearly the whole cell be- comes clear. Structure. — The pancreas belongs to the class of compound racemose glands, and closely resembles IK H&iafofifii Fig. 79.— Section of the pancreas of the dog (Klein), a, termination of duct in the tubular alveoli, a. the salivary glands, though of somewhat looser texture the lobules being separated by more connective tissue. Pancreatic Juice is a clear, viscid, alkaline fluid resembling saliva, but of greater specific gravity, and containing from 2 to 5 per cent, of solid matter. About 12 to 16 ounces are secreted in 24 hours. It contains : — 1. Four ferments : (a) Trypsin, changes proteids into peptones. 1 96 Digestion (b) Pancreatic diastase, changes starch into dextrin and maltose. (c) Curdling ferment, precipitates the casein of milk. (d) Emulsive ferment, emulsifies and saponifies fats. 2. Albumen. 3. Mucin 4. Salts and water. Action i. It changes proteids into peptones in alkaline or neutral solutions, afterwards decomposing them into leucine and tyrosine. 2. It converts starch into dextrin and sugar. 3. It emulsifies and saponifies fats. On Proteids. — Pancreatic juice artificially prepared from pancreas acts in a somewhat similar manner on proteids as gastric juice, but can go further and break up peptone into leucine and tyrosine. It acts ener- getically on some proteids, as the casein of milk, pro- vided the solution is alkaline, but is less active than artificial gastric juice on white of egg. Its solutions require to be alkaline, equivalent to one per cent, of sodium carbonate. Its activity depends upon a ferment called trypsin. Pancreatic digestion of proteids differs from gastric in that (1) it requires an alkaline instead of acid medium ; (2) the proteids are dissolved without the preliminary swelling ; (3) leucin, tyrosin, and similar bodies are formed. On Starch. — Pancreatic juice acts with great energy on raw or cooked starch, quickly converting it into dextrin and maltose. It is more energetic than saliva. On Fats. — Pancreatic juice, when shaken up with fats and oils, reduces the oily matters to a state of fine Large Intestine 197 division and suspends them, forming a milky fluid. It also splits up fats into glycerine and their respective acids. An artificial pancreatic juice can be made by pounding up fresh pig's pancreas with powdered glass, and adding dilute spirit or glycerine. If artificial pancreatic juice be added to milk, coagulation takes place ; if the milk is rendered alka- line by carbonate of soda, before the addition of the pancreatic fluid, no curdling takes place ; and at a temperature of about 35° C. the casein is gradually converted into peptone. Succus Entericus. — This is the secretion of the intestinal glands. It appears to act in a similar way to pancreatic juice. It also contains a ferment which converts cane sugar into invert sugar. Large Intestine The large intestine consists of caecum, colon, rectum. Structure —resembles the small intestine, with some modifications. The Serous coat completely surrounds the in- testine in the transverse colon ; is incomplete else- where. The Muscular coat consists of two layers, the longitudinal being arranged in three flat bands, except at the rectum. One band is posterior, another an- terior, and a third lateral or inferior in the transverse colon ; along the latter the appendices epiploicae are attached. These longitudinal fibres, by being shorter than the intestine, throw it into sacculi, which are marked off from one another by constrictions where the circular fibres are most marked. The circular fibres form a thin layer over the intestine, and are best marked at the constrictions. 198 Digestion The Mucous membrane is lined with columnar epithelium, and is destitute of villi. It has numerous glands of Lieberkuhn and solitary glands, also retiform tissue. The junction of the small and large, intestine is guarded by a valve, and the termination of the rectum by the sphincter. But little, if any, digestive action goes on in the large intestine ; the principal work done is absorption ; the contents of the intestine be- come firmer and harder as they approach the rectum. The contents of the large intestines are acid, from the acid fermentations going on in the faecal matters. Movements of the Intestines If the abdomen of a recently killed animal be opened, the muscular fibres of the intestines will be seen alternately contracting and relaxing, but working down the intestine in waves so as to propel the con- tents downwards. This peristaltic action is increased by the presence of food or bile, or by irritation of the vagus nerve. It is checked by irritation of the splanchnic. The exact nervous mechanism is un- known, but it is probably automatic, like the action of the heart. The movements of the large intestine resemble those of the small ; the feces are lodged in the sacculi during the relaxation of the intestine. Defecation.— The sphincter is normally con- tracted under the influence of a nervous centre in the cord. The sigmoid flexure prevents the feces from pressing too heavily on the rectum. The act of defecation consists in an inhibition of the nervous centre in the cord which governs the sphincter, re- laxation of the sphincter taking place. At the same time a deep breath is taken, the glottis is closed, the diaphragm and abdominal muscles contract, press Summary 199 upon the descending colon, and eject the contents of the rectum, the sigmoid flexure having previously become filled by peristaltic action. Summary of Digestive Changes The essential work of digestion is performed by a singular group of bodies termed ferments. These bodies are found in nearly all the secretions poured into the alimentary canal, and play an exceedingly important part in dissolving the food. These fer- ments are soluble in water, and differ in this respect Table of the digestive juices and their ferments (Roberts). Pancreatic juice Intestinal juice Digestive fluid Saliva . Gastric juice . Ptyalin or Diastase ; a. Pepsin b. Curdling 1 ferment a. Trypsin . b. Curdling I ferment 1. Diastase . \d. Emulsive » ferment Invertin . Action Changes starch into dex- trin and sugar Changes proteids into al- bumose-peptone in the acid solution Curdles casein of milk Changes proteids into pep- tone in alkaline solu- tions Curdles the casein of milk Changes starch into sugar Emulsifies and partly sa- ponifies fat Changes cane sugar into invert sugar 2oo Digestion 6 from the organised insoluble forms, as the yeast plant. They diffuse through animal membranes, though with difficulty ; they are rendered inert by a heat of 70° C. (160 F.), and they are precipated by strong alcohol. Mouth. — The food is crushed, mixed with saliva and reduced to a pulp ; a certain amount of starch converted into maltose and rendered slightly alkaline. Fats and proteids unaltered. Stomach. — Contents rendered acid, conversion of starch into sugar ceases, connective tissue of fats dissolved, and fats set free. Proteids dissolved and peptones formed. The albuminous foods are dis- solved for the most part, and a grumous mixture of peptones, liquid fats, and starches is formed, which is termed chyme, and is gradually passed through the pylorus into the intestine. In the Intestine. — The chyme mixes with the bile, pancreatic and intestinal juices, becomes alkaline, conversion of starch into sugar recommences, emul- sifying of fat begins, and the undissolved proteids are converted into peptones. The diffusible peptones and salts enter the portal vein, the fat in a fine state of division entering the lacteals. In the large intes- tine the liquid chyme becomes more and more solid, is rendered acid by fermentative changes, and acquires the odour of fasces. CHAPTER XIV ABSORPTION AND NUTRITION The food must be acted upon by the various secre- tions of the alimentary canal before it can enter the blood-vessels or lacteals with which the walls of the stomach and intestines are well supplied. Absorption 201 The Albuminous Foods are crushed and re- duced to pulp in the mouth, and converted into the peptones by the action of the gastric, pancreatic, and intestinal juices. By far the greater part of the pep- tones thus formed enter the capillary blood-vessels of the stomach and villi. Being diffusible through animal membranes, they pass through the walls of the capillaries by osmosis, enter the portal vein, and are conveyed to the liver. In the liver they are either split up into more oxidised bodies, as glycogen, urea, or kreatin, or are reconverted into albumen to assist in the nutrition of the tissues. In the present state of our knowledge it seems uncertain where or how peptones undergo change, whether before reaching the liver or in their passage through the liver. Some observers have failed to find peptones in the portal vein. The Starches are converted into dextrin and sugar by the action of the saliva, pancreatic, and intestinal juices, and being thus rendered diffusible enter the portal vein, and are conveyed to the liver. The liver probably converts the sugar into glycogen, and stores it up till required to be oxidised for the production of heat and muscular energy. A variable amount of sugar appears to be converted into lactic acid in the intestines. The Fats are crushed and reduced to pulp in the mouth, and their fibrous tissue and vesicular envelopes dissolved in the stomach, so that the oily matters are set free. In the small intestine they undergo two different changes, which are effected by the secretions of the small intestine : — 1. They are emulsified. 2. They are saponified. The emulsification consists in reducing the fat into fine particles, small enough to readily enter the 202 Absorption and Nutrition lacteals. The saponification consists in the formation of soaps : thus olein is decomposed, the glycerine being set free, and the oleic acid forming an oleate with sodium or potassium for a base. Small quantities of the fatty matters find their way into the portal vein, but by far the major quantity enters the lacteals of the villi. The particles of fat enter the protoplasm of the columnar cells surround- ing the villi, so that if these cells be examined during a period of digestion they are seen to be distended with fat particles. They next pass into the retiform tissue of the villi, and thence into the lacteal, which commences in the villus. Finally, the fatty matters forming the chyle pass through the mesenteric glands and into the receptaculum chyli and thoracic duct. The exact forces in operation which determine the entrance of fat into the lacteals are not thoroughly understood. Animal membranes Wetted with bile much more readily allow fatty matters to pass through them than membranes not so treated. The cells surrounding the villi, perhaps, exercise some selective power, as the glandular epithelium does in the con- voluted tubes of the kidney. The fat, once within a villus, is driven onwards by the contraction of the mus- cular fibres present in the villi, compressing the lacteal and forcing onwards its contents, the aspirating power of the thorax supplying the vis a fronte. The fatty matters and albuminous materials present in the chyle are gradually, in part, converted during its passage through the mesenteric glands into the elements of fibrin and white blood-corpuscles. The food that has entered the body in the form of meat, starch, sugar, fats, after being digested passes into the blood-vessels in the form of peptones, fatty matters, and sugar. What processes must they un- dergo before they become formed tissue, such as muscle, nerve, tissue, or gland ? But very little is The Liver 203 known of such changes. Some of the albuminous and fatty matters are converted into white corpuscles and fibrin, probably through the action of the blood- glands, i.e. spleen, lymphatic, lenticular, tonsils, thy- mus glands ; the white corpuscles passing into red or exuding into the tissues to become transformed into the actual cells or fibres of the various tissues. The albumen, fats, and sugars absorbed from the alimentary canal pass out of the body at the kidneys and lungs as urea, salts, and C0 3 . About the inter- mediate stages our knowledge is scanty. Summary Peptones (major part) Sugar The portal vein absorl is Salt Soaps ,, Fats (trace) ^ Water (major part) The lacteals ab- _ sorb Fats (major part) Soaps (small part) Peptones ,, Sugar (trace) Salts „ MVater (small part) CHAPTER XV TH E LIVER The liver is the largest gland in the body, and weighs 50 to 60 ozs. In the foetus and child it is larger in proportion to the body-weight than in the adult, being 1 in 20 to 30 in the child and 1 in 40 in the adult. 204 The Liver The liver receives the blood of the portal vein and hepatic artery, the hepatic veins carrying away the blood from trie organ. Its under surface is divided into lobes by five fissures. The Fissures are the transverse, where the vessels and nerves enter ; the longitudinal, situated between the right and left lobes, is divided into two by the transverse fissure, the anterior part forming the um- bilical fissure and containing the round ligament, and the posterior the fissure of the ductus venosus, con- taining the obliterated remains of the ductus venosus of the foetus. The fissure of the gall-bladder, or rather fossa, makes the fifth. The Lobes are, right and left, separated by the longitudinal fissure. The lobulus quadratus situated between the gall-bladder and longitudinal fissure. The lobulus Spigelii, between the fissure for the ductus venosus and inf. vena cava. The lobulus caudatus forms a sort of ridge extending from the base of the Spigelian lobe to the under surface of the right lobe. Structure. — The liver has two coverings, the serous and fibrous coats. The Serous is derived from the peritoneum, and is reflected round the organ, except where the vessels enter, and at the posterior border. The Fibrous or Connective Tissue coat in- vests the whole gland, and at the transverse fissure becomes continuous with the fibrous tissue which accompanies the blood-vessels into the substance of the liver, and forms the capsule of Glisson. Hepatic Lobules.— On section of the liver its substance will be seen to be composed of closely- packed bodies of rounded outline and of about i to 2 mm. (j\j to „ J | T inch) in diameter. These lobules for the most part have a darkish-red centre and lighter circumference, and in some animals, at least, are separated by a small quantity of connective tissue Hepatic Cells 205 The centre of the lobule is occupied by an intralobular vein, which collects the blood from the capillaries of the lobule and empties itself into the sublobular ; the latter opens into the hepatic veins. The circum- ference of the lobule is surrounded by the interlobular veins, which are branches of the portal system ; capil- laries passing from the circumference to the centre of the lobule connect the interlobular veins with the intralobular. The Hepatic Artery enters the liver at the transverse fissure, accompanies the portal vein and Fig. 80. — Section of a portal canal (Schafer). a, branch of hepatic artery; v, branch of portal vein ; d, bile duct; //, lymphatics. ducts, and supplies the connective tissue of the liver (fig. 80, a). The Hepatic Cells are packed in between the network of capillaries in the lobule. They are of rounded or polyhedral form, ¥ J- U - to T -^ v - ff inch in dia- meter. They have a yellow granular appearance and a well-marked prominent nucleus. Whilst in a 206 The Liver quiescent state the liver-cells are larger and more granular than after action. On examining the cells by a high power, they may be seen to contain a fine network which extends into the nucleus. They are joined together by an albuminous cement, which contains fine channels for the bile-capillaries ; during digestion they contain minute oil-globules and gly- cogen. The Biliary Ducts commence by a fine plexus of capillaries which run between and surround the cells (fig. 81). In a very thin section minute openings may be seen between the cells, which are the aper- tures of the capillary ducts. The larger bile-ducts are lined with columnar epithelium, their coats being formed of fibrous and elastic tissue, with a mixture of unstriated muscular fibre. The branches of the portal vein, artery, and duct accompany one another through the liver, the hepatic veins travelling by themselves (fig. 80). Functions of the Liver The portal vein carries the blood which has circu- lated through the walls of the stomach and intestines, pancreas, and spleen. It is loaded with material absorbed from the contents of the stomach and in- testines. This blood is submitted to the liver before entering the general circulation. In its circulation through the liver it enters the interlobular plexus, travels through the capillaries of the lobule, coming into close relation with the hepatic cells, enters the intralobular veins, and finally the hepatic veins convey it to the inferior vena cava. The liver in the adult has at least three func- tions : — 1. Formation of glycogen. ? Conversion of gly- cogen into sugar. Glycogen 207 2. Action on albuminous substances. 3. Secretion of bile. 4. In the foetus it appears to give origin to white blood-corpuscles. 1. Glycogen, or Amyloid Substance (C 6 H 10 O s ), is present in the cells of the healthy liver. The liver contains from 1^ to 2\ per cent. When pure Fig. 81.— Section of rabbit's liver with the intercellular network of bile- canaliculi injected (Hering). Two or three layers of cells are represented. I?, capillaries. it is a white, tasteless, inodorous powder, insoluble in alcohol, soluble in water, forming a white opalescent solution. It closely resembles starch in appearance, but differs from it in being stained reddish-brown by iodine. Like starch, it is readily converted into sugar by the action of dilute acids or ferments. Besides 208 The Liver being present in the liver it is found in living muscle, white corpuscles of the blood, brain, placenta, and most tissues of the fcetus. Preparation. — Fresh liver is boiled with strong solution of KHO, which dissolves the liver-tissue and the glyclogen, and on pouring it into alcohol the gly- cogen is precipitated tolerably pure. Another method consists in making a decoction of liver, precipitating the albuminous matters with potassic hydrarg. iodide and HC1, and afterwards precipitating the glycogen with alcohol (see p. 13). Origin. — Glycogen is principally formed in the liver from the saccharine elements of the food. C 6 H 12 O b -H 2 O=C 6 H I0 O 5 Grape sugar - Water = Glycogen, Dogs fed on starch or sugar rapidly accumulate large quantities of glycogen in the liver ; when fed on a purely animal diet very much smaller quantities are found. This appears to show that while glycogen is formed in small quantities from albumen, yet by far the major part originates from the saccharine elements of the food. In badly nourished or half-starved animals no glycogen is found. Fatty foods do not increase the amount. Destiny of Glycogen. — The fate of glycogen is un- certain. There can be no doubt it serves to store up material rich in C and H ; but the exact manner in which it is utilised is not fully understood. Bernard maintained that it is gradually reconverted into sugar as required, and oxidised in the capillaries of the body to maintain the heat or to supply muscular energy. He based this view on his analysis of the blood, which showed that a larger quantity of sugar existed in the hepatic than in the portal vein. He also found a greater quantity in the arteries than in the veins, which seemed to suggest that sugar disappeared in Functions of Liver 209 the capillaries. Pavy maintains that the hepatic veins during life only contain a trace of sugar, and the arteries contain no more than the veins. He argues that the large quantities of sugar found in the hepatic veins after death are due to a post-mortem change of glycogen into sugar, and that during life only traces are to be found. He does not believe that glycogen is reconverted into sugar during life, and that if it were in any quantity, it would run off at the kidneys, as in diabetes. The question is still subjudice. Recent analyses, however, show that the amount of sugar in portal blood is 1 per 1,000, in the blood of the hepatic veins 2 per 1,000 : this certainly suggests that glycogen is converted into sugar in the liver, and that glycogen is stored in the liver as a sort of carbohydrate reserve. It is probable that the sugar in the blood is con- sumed by active muscle, and is discharged as CO, and H 2 0. Diabetes is a disease characterised by an abnor- mal quantity of sugar in the urine. Its immediate cause is a rapid conversion of glycogen into sugar in the liver, depending probably on some disturbed innervation of the blood-vessels. It can be induced artificially in animals by puncture with a needle of the vaso-motor centre of the medulla. This leads to dilatation of the blood-vessels of the liver, an in- creased supply of arterial blood, and an increased conversion of glycogen into sugar, which makes its appearance in the urine. 2. Action on Albuminous Substances. — (a) Preparation of the "peptones for assimilation. (6) Splitting up of various bodies into urea, &c. (a) The portal vein contains the peptones which have been absorbed from the alimentary canal. These bodies disappear during their passage through the liver, being probably converted into serum-albumen. p 210 The Liver (b) The liver probably splits up various substances, as albumen, kreatin, leucin, and tyrosin, the products being glycogen, urea, and uric acid. In certain diseases, as acute yellow atrophy of the liver, the urea in the urine is lessened, and tyrosin and leucin appear to take its place. 3. Secretion of Bile. — In all probability the pigments and biliary acids are formed in the liver, and not merely separated from the blood. No trace of either of them has been found in frogs whose livers have been extirpated. To what extent the secretion of bile gets rid of effete matters from the system is uncertain. The biliary acids are in large part re- absorbed after having taken part in the digestion of the contents of the small intestine. 4. Foetus. — The relative size of the liver in early fcetal life is about half the body-weight ; at full time it is about 1 in 18. It receives blood from two sources — (a) the umbilical vein, a portion of which escapes through the ductus venosus directly into the inf. vena cava ; (b) the portal vein, which carries venous blood resembling that of the body generally. The functions of the fcetal liver differ from those of the adult principally in its being a blood-making organ. After the formation of the placenta the um- bilical vein brings various nutritive materials from the maternal system ; the liver seems, out of these materials, to produce numerous colourless nucleated corpuscles which are poured into the blood. Probably before birth it ceases to do so, the spleen and lymphatic glands taking its place. The biliary secretion (meconium) of the foetus is purely excrementitious in character. The Kidneys 2 r 1 CHAPTER XVI THE KIDNEYS The kidneys are situated in the lumbar region, opposite the last dorsal and two or three upper lumbar vertebrae. They are about 4 inches in length, and weigh 4 to 5 oz. each. Structure. — The kidneys are invested by a thin fibrous capsule, which is attached by connective tissue and blood-vessels. It is easily stripped off. On making a longitudinal section through a kidney the glandular structure will appear to be divided into two portions : (1) the outer or cortical portion, for the most part occupying the surface, except at the hilus ; (2) the medullary portion, consisting of a number of pyramids separated from one another by cortical sub- stance. 1. The Cortical Substance occupies the greater part of the gland, being ^ to \ inch . in depth at the surface, and extends into the centre of the gland between the pyramids ; the cortical portion between the pyramids being termed the columns of Bertini. It is of a light red colour, and more or less dis- tinctly striated in appearance, the striations being due to the interlobular vessels and bundles of straight tubes which pass from the base of the pyramids to the capsule. The Malpighian bodies may be seen as reddish points, or sometimes standing out from the surface like grains of sand. The bundles of straight tubes are called the medullary rays (fig. 82, m), 2. The Medullary Portion occupies the centre of the gland, and consists of eight to twelve of the pyramids of Malpighi. These pyramids are sur- p 2 212 The Kidneys rounded at their bases and sides by cortical sub- stance, while their apices project into the dilated portion of the ureter at the hilus, which forms the pelvis. The pyramids are divided by Ludwig into two layers or zones — the boundary layer (fig. 82, g), and the. papillary layer (p). The boundary layer has well-marked stria- tions, this appearance being due to the vasa recta (fig. 84, ab, vb), and to the bundles of tubules (fig. 82, h) passing down the pyramid towards the papilla. The papillary layer is of a more uniform dull red colour. The Malpighian Bodies are about ,-Jj inch in diameter, and are situated in the cortical portion between the fig. 8=.-Sectioii through part of medullary rays. They the dog's kidney (I.udwig). r, cor- consist of a tllft of capil- tical layer ;g, boundary layer ;fi, pa- , . *• pillary layer ; /;, bundles of tubules lary vessels in a Capsule in the boundary layer prolonged f orme( i Dy tne dilated end into the cortex as the medullary ; J rays, m ; i, spaces containing vasa of a Urinary tubule. The recta (not represented in the figure) ; f r ■ terme( l j-Ug a-/ ome _ c, portion of cortex between the luu ls tciuicu tltc gtumt medullary rays containing inter- rulltS, the membranous lobular vessels, glomeruli, and con- ^ t> ) x voiuted tubes. envelope Joowmans cap- sule. The glomerulus receives an arterial twig from an interlobular artery, and its efferent vein joins a plexus which surrounds the convoluted tube before joining an interlobular vein. Bowman's capsule consists of a homogeneous membrane, lined by flattened epithelium, and joins a convoluted tube by a constricted neck. Urina ry Tub ules 213 The Convoluted Tubes (fig. 83) commence as capsules in the cortex, twist upon themselves several times, then, on joining a medullary ray, take nearly a CURBED COLLECTING TUBULE PROXIMAL) CONVOLUTED?' TUBULEj STRAIGHT - * COLLECTING-)- TUBE/ DESCENDING L1MB\ OF HENLE'S LOOP/ 1 Fig. 83.— Diagram of the course of the uriniferous tubules (G Anatomy). ■rays 214 The Kidneys straight course. They have a distinct lumen, and are lined by short columnar cells, narrower at the lumen than at the basement membrane. The outer part of the cell is distinctly striated ; their inner part is granular. They have a well-marked nucleus. The convoluted tubule passes into a spiral tubule of Schachowa, which is situated in the medullary rays ; these are more or less spirally arranged : the epithelium is the same as in the convoluted tubes. The spiral tubule on entering the boundary layer becomes narrower, and forms the descending loop-tube of Henle ; the epithelium is 'here flattened, with a prominent nucleus. The ascending loop-tube, which has re- entered the boundary layer, is wider than the descend- ing, and is lined by a layer of polyhedral fibrillated cells. It next enters the cortex, is somewhat narrower and wavy, and passes upwards in a medullary ray. It then leaves the medullary ray and forms an irregular tubule. Its shape is irregular, its lumen small, and its epithelium consists of short, columnar, fibrillated cells. The irregular tubule passes into the second convoluted tube, closely resembling the "first convoluted tube. It then passes into a more or less wavy collecting tube before it joins a straight collecting tube. The collect- ing tubes commence in the cortex and pass through the boundary layer into the papillary layer, are lined by columnar or cubical epithelium, and have a distinct lumen. Blood-vessels. — The renal artery, on entering the kidney, breaks up into numerous primary branches, which travel along. the columns of Bertini, and are called the arteria propria renales. These divide at the base of the pyramids and form arches with their neighbours ; these arches give off (i) branches into the cortex termed the interlobular arteries, from which the afferent vessels to the Malpighian tuft arise (fig. 84) ; the efferent vein from the glomerulus breaks Fig. 84.— Diagram of the distribution of the blood- vessels in the kidney (Lud- wig), ai, ai, interlobular arteries ; vr\ vz } interlobu- lar veins; g, glomerulus; vs, stellate vein ; ar, vr-, artenae et venee rectce forming bundles, ab and vb\ vp } venous plexus in the papillae. 216 The Kidneys up into a capillary network which ramifies on the urinary tubules in the cortex, and after an extended course joins the interlobular veins ; the efferent vessels of the lowermost glomeruli break up into capillaries, which pass straight down into the boundary layer, and surround the straight tubules : (2) branches downwards into the pyramids running between the bundles of collecting tubes, and termed the vasa recta or arterice recta. The Renal Veins. — The vence iiiterlobulares cor- respond with the arteries, and receive some veins termed stellate from beneath the capsule, and also the small veins which receive the blood from the minute plexus surrounding the convoluted tubes. The vence rectce run along the pyramids accom- panying the corresponding arteries. The vence proprice renales pass along the columns of Bertini after having been joined by the vena? inter- lobulares and vena? recta?. Pelvis and Ureter. — The ureters convey the urine to the bladder, the upper dilated portion form- ing the pelvis. The pelvis is divided into two or three primary divisions, and these again divide into shorter ones termed calicesox infundibula, which receive the papilla? or apices of the pyramids of Malpighi. The collecting tubes open at the papilla? and discharge their contents into the pelvis. The pelvis and ureters are lined by transitional epithelium. Urine The urine is a clear yellow fluid of specific gravity 1020, of peculiar odour and acid reaction. It is con- stantly being secreted by the kidneys, and is collected in the bladder. On an average 52 ozs. (1,500 c.c.) are passed per diem. The solids amount to about 4 per Urine 217 cent. The principal constituents are the following, with their amounts in 24 hours : — Urea . . 500 grs., or 33 grammes=2 - 2 per cent. Uric acid . 7-8 „ -5 gramme = '03 „ Kreatinin . 14 „ "9 „ Hippuric acid 6 ., -4 ,, Chlorides . 105 „ 7 „ Sulphates Phosphates Sodium Potassium \ Smaller quantities. Ammonia Earthy salts Pigment, &c. Urea. — Properties, tests, &c. (see p. 3). Quantity of the Urine. — The average quantity of the urine amounts to about 50 ozs., or 1,500 c.c. The amount varies at different ages and under dif- ferent circumstances. In infants the amount passed is about 10 ozs. in the 24 hours. In children gene- rally the amount passed is less than in adults. The temperature and moisture of the surrounding atmo- sphere by their action on the skin greatly influence the secretion of urine. In a Turkish bath, or in summer, where the skin is acting freely, small quantities of concentrated urine are passed, while, on the other hand, exposure to a dry east wind causes the secretion of large quantities of pale limpid urine. The amount of fluid ingested, as well as an increased or decreased blood-pressure, influences the amount of the urine. Colour of the Urine. — The colour varies according to the degree of concentration of the urine. The pale golden yellow colour is caused by the presence of a pigment, urobilin, derived from the haemoglobin of the blood. In some conditions of anaemia the urine is pale though its solid contents 218 The Kidneys may not be low. In children it is paler than in adults. Specific Gravity. — The average specific gravity is 1020, though it may vary from 1002 to 1040, ac- cording to the amount of fluid ingested as well as the amount of perspiration taking place. In infants the specific gravity is 1003 to 1006. Acidity.— Normal urine is slightly acid. This acidity is not due to the presence of free acid, but to the presence of acid phosphates. This acidity is in- creased after muscular exercise and much animal food. After much vegetable food, or organic acids, as tar- trates and citrates or alkalies, the urine becomes neutral or alkaline. Sources of Urea. — The greater part of the effete nitrogen of the body passes out of the system in the form of urea, a much smaller quantity in the uric acid, kreatinin, hippuric acid, and other minor constituents. The stages of the process by which proteid matters are converted into urea are not well understood. It is probable that leucin, tyrosin, kreatin, glycin, uric acid, hippuric acid, which are nitrogenous bodies less complex than proteid, and more complex than urea, may be intermediate stages between proteid and urea. It must not be supposed that all the urea secreted passes through all these stages. The two most probable sources of urea are (1) from kreatin ; (2) from leucin and tyrosin. 1. Kreatin is found in the blood, and in most ot the tissues of the body — muscle contains from -2 to •4 per cent. ; while urea does not exist in muscle, and only to a very small extent in the various organs. It is possible that kreatin represents the waste product of the albumen of the tissues ; that, in consequence of the changes necessitated by life, there is a constant formation of kreatin in all the tissues of the body, and that this kreatin passes into urea in the blood, Urea 2 1 9 the liver, or in the kidneys. The small increase of urea in the urine after active exertion would, on this view, represent an increased wear and tear, leading to an increased formation of kreatin and urea. 2. If the amount of nitrogenous food be increased in quantity, the amount of urea excreted in the urine is also increased. This would indicate that a certain part of the albumen of the food is split up into urea, &c, without its having taken part in the formation of any tissue. Leucin and tyrosin are found in the small intestine, and are formed when pancreatic juice acts upon albuminous foods. It is probable that the leucin and tyrosin enter the portal veins, and are con- verted into urea in the liver. This is rendered the more probable from the fact already mentioned, that in acute yellow atrophy of the liver, leucin and tyrosin replace urea in the urine. Amount of Urea — About 500 grains of urea escape through the kidneys during 24 hours ; but this amount varies according to circumstances, the amount being increased after large quantities of animal food, slightly after exercise, and also during fevers. The urea is diminished after vegetable food or fasting, and in certain forms of kidney disease. Urzemia. — In certain conditions of the body, such as Bright's disease, and in fevers, there is a greater accumulation of effete material in the body than can be carried off by the kidneys. Certain toxic effects, such as convulsions and coma, result. This is probably, though not certainly, due to the accumulation of urea in the blood. Estimation of Urea. — There are two methods : (1) Liebig's ; (2) Russell and West's. 1. Liebig's method depends upon the fact that urea forms an insoluble precipitate with mercuric nitrate. Before the estimation can be made, the sul- phates and phosphates present are precipitated by 220 The Kidneys baryta water, and the liquid filtered. A certain quan- tity of the filtrate is taken, and a solution of mercuric nitrate of known strength (10 c.c. = 'i gramme of urea) is dropped into the urine with frequent agitation ; a white precipitate falls. From time to time, as the mercuric solution is added, a drop of the liquid is tested on a white slab with a drop of solution of sodic carbonate ; when all the urea is precipitated and free mercuric nitrate present in the solution, a yellow precipitate occurs with the sodic carbonate. The amount of mercuric solution added is read off, and the corresponding amount of urea estimated. If great accuracy is required, the amount of CI present must be estimated, and an allowance made in esti- mating the urea, as no precipitate of urea occurs until all the chlorine present has combined with the mercury. 2. Russell and West's method depends upon the fact that urea is decomposed by hypobromous acid into C0 2) N, H. 2 0. The C0 2 is absorbed in passing through a solution of NaHO and the N measured in a graduated tube (see fig. 85). The amount of N given off indicates the amount of urea present in the urine (see p. 5). Uric Acid.— Some 7 to 8 grains of uric acid are excreted daily in the urine, for the most part in the form of urates of potassium, sodium, or ammonium. The amount varies, being increased after animal diet, and in certain diseases, as gout. Its source is uncer- tain, being, like urea, a waste product formed from the breaking up of nitrogenous compounds. It is probable that in certain derangements of the liver uric acid is formed instead of urea. It represents a less oxidised form than urea (see p. 5). Kreatinin. — Some 14 grains daily of kreatinin escape by the kidneys. Probably the greater portion of kreatin formed in the body has been converted Hippuric Acid 2 2 1 the into urea, a small amount being converted into kreatinin which escapes with the urine (see p. 7). Hippuric Acid.— Only about 6 grains of hip- Fig. 85. — Gerrard's apparatus for estimating the amount of urea in urine. a is a wide-mouthed bottle connected to b by means of a flexible tube and T-shaped glass tube ; b is a cylindrical vessel graduated so as to measure the amount of gas given off from a ; c is a vessel connected to b by a flexible tube below, and arranged to slide up and down. To use the apparatus both c and b are nearly filled with water, and c is raised or lowered so that the water stands at a level marked o on b ; 25 c.c, of a solution of hypobromite of soda is placed in the bottle «, and 5 c.c. of urine in a test-tube, the latter being placed without spilling, by means of forceps, in a, and the india-rubber stopper placed in the bottle. On shaking the latter so as to mix the urine and hypobromite solution, nitrogen gas is evolved, the carbonic acid being absorbed by the excess of soda present. The gas collects in the graduated cylinder, and the amount is indicated by the marks on the side. The reservoir c is lowered so that the water in both stands at the same height before being read off. The hypobromite solution is made by adding 25 c.c, of bromine to 250 c.c. of a 40 per cent, solution of NaHO. 222 The Kiditeys puric acid are secreted daily in man, though a very much larger amount is present in the urine of the herbivora (see p. 6). Pigments. — The yellow colour of the urine is due to several pigments, urobilin, urochrome, urorubin, the nature of which is ill understood. Indican also occurs in the urine in variable quantities ; it is known by the presence of a blue colour, due to the formation of indigo, when strong acids are added to the urine (see p. 9). Inorganic Salts. — These are numerous, the most abundant being sodium chloride, smaller quan- tities of potassium, magnesium, calcium in the form of phosphates, sulphates, chlorides, and carbonates. The amount and variety of the salts in the urine differ according to the food taken, the alkalies being increased during a vegetable diet, the urine becoming alkaline, the earthy salts being increased when animal food is taken.- Secretion of Urine The Malpighian bodies, and that portion of the urinary tubules known as the convoluted tubes, are both engaged in the separation of the urine from trie blood. The Malpighian bodies, as before explained, consist of a tuft of capillaries fitting inside a capsule communicating with the convoluted tubes, and are probably engaged in secreting the greater part of the water and inorganic salts of the urine, the process depending to some extent upon the pressure in the capillary tuft. The convoluted tubes are lined by glandular epithelium, and are surrounded by a plexus of ca- pillaries. The epithelium lining them appears to exercise a certain selective influence in secreting the urea and uric acid and pigment. These substances, Secretion of Urine 223 having been separated from the blood and entered the urinary tubules, are washed down by the water and salines transuding through the capillary tufts into the capsule above. Secretion of Urine by the Glomerulus. — The amount and character of the urine largely depend upon the blood-pressure in the glomeruli of the kidney. If the pressure be increased, larger quantities of water will be passed, and under certain circumstances,- as in nephritis, albumen and blood. The passage, however, of fluid from the capillary tuft is not a mere filtration, inasmuch as the urine must pass through the wall of the 'capillary and through the epithelium covering it. Injury to, or an alteration in, the epithelium may possibly allow albu- men or other constituents of the blood to pass. The experiments of Roy have shown that the volume of the kidney readily undergoes change, the dilatation of the arteries causes increase, and the con- traction of the arteries decrease, of volume. If this alteration in volume be registered by a suitable apparatus on a moving surface, a curve will be pro- duced resembling the blood- pressure curve, both respiratory undulations and pulse being well marked. The arteries of the kidney are supplied with vaso- motor nerves, and thus the blood-supply to the kidney and the blood-pressure in the glomeruli are regulated. As the amount of urine depends so largely on the blood-pressure in the glomeruli, it will be well to state concisely the conditions upon which this depends. According to Foster the blood-pressure in the glome- ruli is increased — 1. By an increase of the general blood-pressure, brought about (a) by an increased force of the heart's beat ; (b) by the constriction of the arteries supplying the skin or other part. 224 The Kidneys 2. By a relaxation of the renal artery. It may be diminished — i. By a lowering of the general blood-pressure (a) by diminished force of the heart's beat ; (b) by a dilatation of the arteries of the skin or other areas. 2. By a constriction of the renal artery. Section of the renal nerves causes a dilatation of the renal arteries and a copious secretion of watery urine. Stimulation of the renal nerves has an oppo- site effect. Section of the splanchnic nerves is followed by an increased flow. Stimulation arrests the flow. Stimulation of a sensory nerve produces constric- tion of the renal vessels and diminished urine. Section of the cord, below the medulla, leads to a general dilatation of the arteries of the body, lowers the general blood-pressure, and arrests the secretion of urine. Stimulation of the cord produces a similar effect by constricting the renal arteries. The rise in general blood-pressure is not sufficient to overcome the re- sistance offered by the constricted renals. Injection of urea into the blood causes first a con- striction, and then a dilatation, of the renal arteries, followed by an increased flow of urine. Some diuretics, as sodium acetate, cause a dilata- tion of the vessels and an increased flow at once. These diuretics appear to act directly on the vessels, as they increase the urine after the nerves are divided. Secretion by the Renal Epithelium. — Whilst the secretion of the watery and saline constituents of the urine takes place at the glomerulus, and is largely dependent upon blood- pressure, the urea, uric acid, and other substances present in the blood are ap- parently secreted or separated from the blood through the agency of the epithelium lining the convoluted Secretion of Urine 225 tubes. Even after the medulla has been divided, and the urine, in consequence, has ceased to flow, the in- jection of urea, sodium acetate, and some other bodies, is followed by a free secretion of urine. Blood con- tains about '02 per cent, of urea in health, and as much as '2 in the urasmia of nephritis, while urine contains 2 per cent. It is evident that the epithelium of the kidneys is actively employed in selecting the urea in the blood and passing it into the urine. Heidenhain's experiment with indigo-carmine also shows this. The spinal cord of a rabbit is divided so as to lower the blood-pressure, a solution of indigo- carmine is injected into the blood, and the rabbit is killed an hour later. The cortical layer of the kidneys is found blue in colour, which is due to the presence of blue granules in the epithelium and in the lumen of the convoluted tubes and the ascending lines of Henle's loop ;- the capsules of the glomeruli are free from the blue granules. From these facts it appears probable that the secretion of urine takes place both through the medium of the glomerulus and through the capillary plexus and the epithelium lining the convoluted tubes. The primary work carried on by the former is to discharge water from the blood, and flush, as it were, the convoluted tubes, the amount of urine se- creted being dependent upon blood-pressure. The primary office of the epithelium lining the convoluted tubes is to select certain substances circulating in the blood, and secondarily a secretion of sufficient water to enable them to pass through the epithelium and tubes. The pressure under which urine is secreted has been determined in the dog by placing a manometer in the ureter. It amounted to 60 mm. of mercury, the pressure in the aorta at the same time being 100 mm. The Kidneys The Urinary Bladder The bladder has an average capacity of about 20 ozs., but is capable of becoming distended to a much greater extent. It is situated in the pelvis, its base or fundus being seated on the rectum or vagina in the female. When completely distended its apex rises out of the pelvic cavity. Structure of the Bladder. — The bladder is com- posed of a serous, muscular, submucous, and mucous coat. The Serous coat only partly invests it, covering the upper half or more of the posterior wall, and being reflected from the sides and apex to the sur- rounding parts. The Muscular consists of unstriated fibres ar- ranged in three layers. The external or longitudinal is most distinctly marked on the anterior and posterior surfaces of the organ. This layer forms what is sometimes called the detrusor urince, muscle. The circular or middle layer surrounds the bladder in a more or less oblique direction ; it is more circular in direction towards the base and around the neck ; it consists of a dense layer of fibres, which forms the sphincter vesica. The internal layer is thin and more or less longitudinal in direction. The Submucous coat is formed of connective tissue and blood-vessels. The Mucous Membrane is pink and smooth ; it is thrown into wrinkles or rugae, except at the trigone, where it is adherent to the muscular layer beneath. It is lined by transitional epithelium (p. 26) re- sembling that of the ureters, and consisting of three layers, the most superficial being cubical ; the second Micturition 227 more or less pear-shaped, fitting into the layer above ; the deepest more or less rounded or oval, sending processes into the mucous membrane beneath. Micturition The urine trickles drop by drop down the ureters, into the bladder, where it collects and gradually dis- tends it. The exit from the bladder is opposed by the sphincter vesicae, or, as some believe, by the elastic and muscular fibres of the urethra. The urine is expelled by the contraction of the walls of the bladder, more especially by the detrusor vesicas, and is assisted when much distended by the contraction of the abdominal walls. Micturition is a reflex act, but one which (with the exception of infants) is under the influence, if not under the control, of the will. The mechanism appears to consist of an automatic centre in the lumbar part of the cord, maintaining the constant contraction of the sphincter (fig. 86, m s), a second centre, which when stimulated excites con- traction in the detrusor u d. These centres are anta- gonistic, so that an afferent impulse from the bladder s excites the detrusor centre, at the same time inhibiting the sphincter centre. These centres are normally under the control of the will, so that although an afferent impulse may ascend from the bladder to the centres of the cord and up to the sensorium, yet by an effort of will the sphincter centre may be assisted and the detrusor inhibited, or vice versa. Thus when the bladder becomes distended an afferent impulse from the blad- der reaches both lumbar centre s and brain ; if the opportunity is favourable for micturition the controlling Q 2 228 The Kidneys influence of the will is removed, the reflex contraction of the detrusor taking place, assisted perhaps by the voluntary abdo- minal muscles. In animals when the cord is divided, or in man injured above the lumbar centre, so that the controlling in- fluence of the will is cut off, an involuntary emptying of the bladder takes place. In chil- dren, when sleeping heavily, or when the spinal centres are un- duly irritable, a dis- tended bladder or the irritation of worms in the bladder or rectum will cause incontinence of urine. In animals Fig. 86. —Diagram showing probable plan of the centre for micturition (after Gowers). M T, motor tract ; s t, sensory tract in the spinal cord ; MS, centre, and ms, motor nerve Or man, when the luill- for sphincter ; M D, centre, and md, U„ r r p n (- rpt . arp i n ii, rP H motor nerve for detrusor; s, afferent Udf i ( - Lncres *« injured nerve from mucous membrane to s, sensory portion of centre;?, bladder; at r the condition during rest is indicated, the sphincter centre in action, the detrusor centre not . acting ; at a the condition during quence Ot relaxation 01 action is indicated the sphincter the sphincter. At other centre inhibited, the detrusor act- . r ing. times urine accumu- lates, distends the blad- der, and then escapes in drops, from failure of the detrusor to act. or destroyed, the urine may dribble away as it is formed, in conse- The Ductless Glands 229 CHAPTER XVII THE DUCTLESS GLANDS The spleen, lymphatic glands (including lenticular glands of alimentary canal and tonsils'), supra-renals, thymus and thyroid, form the ductless glands. The pituitary, peneal, and coccygeal bodies are not in any sense glands, nor have they probably any analogous function to them. Spleen The spleen is the largest and most import ant of the ductless glands. It is a soft, red, vascular organ, situated at the cardiac end of the stomach beneath the diaphragm. It has two coats, a serous and fibro- elastic. The Serous closely invests its surface except at the hilus and at the spot where it is reflected to the stomach and diaphragm. The Fibro-elastic or Tunica propria is a strong capsule surrounding the organ, and, passing into its substance at the hilus, forms a sheath for the vessels and trabecule, which divide the gland into spaces occupied by the pulp. It consists of white and yellow fibrous tissue, and non-striated muscular fibre, the latter well marked in the pig and dog, but more scanty in man. The capsule is highly elastic, and capable of great distension. The Spleen Pulp occupies the spaces between the trabeculae, is of a dark-red colour, and semi-solid consistence. The pulp, when examined in thin section beneath the microscope, is seen to consist of a network of branched connective tissue corpuscles (fig. 87, p) — 230 The Ductless Glands the supporting cells of the pulp — the branches joining one another, and forming a fine retiform tissue. Many of these connective tissue corpuscles contain a clear oval nucleus, and some contain yellowish pigment granules, possibly derived from the blood-corpuscles, The spaces between these branched cells contain (i) red corpuscles, (2) white corpuscles of various sizes and more or less granular, (3) transitional forms between red and white corpuscles, (4) cells containing red corpuscles, (5) pigment granules. Fir,. 87. — Thin section of spleen pulp, showing the origin of a small vein (Quain's Anatomy). 7<, vein filled with blood corpuscles, which are in continuity with others, />l, filling up the interstices of the retiform tissue of the pulp. At / the blood corpuscles have been omitted from the figure, and the branched cells are better seen ; w, wall of the vein. The Splenic Artery enters the spleen by divid- ing into six or more branches, which ramify in the interior, supported by the trabecular, and break up in the pulp into fine branches. The small arteries ter- minate in capillaries, the walls of which eventually are lost, their cells becoming gradually transformed into the connective tissue corpuscles of the pulp, and their contained blood wanders freely through the Spleen 2 3 1 retiform tissue of the pulp. The minute veins arise in a manner similar to that in which the arteries terminate and eventually empty themselves into the splenic vein. Thus the blood in its course through the spleen, after leaving the arteries, wanders freely through the pulp before entering the veins (fig. 87). The terminal arterial branches do not anastomose with one another. The Malpighian Corpuscles are small bodies, about tjV in. in diameter, and may readily be seen in the child's spleen as small white dots scattered thickly over the cut surface. They are seated upon the small arteries, their sheath being continuous with that of the arteries, though in man the sheath is not very distinct, and the tissue of the Malpighian body is continuous with that of the spleen pulp. In structure they con- sist of lymphoid tissue, the leucocytes being densely packed in a fine network. A small artery enters their substance. Functions. — The exact functions performed by the spleen in the animal economy are ill understood ; the most certain are the following : — (1) During digestion the spleen becomes con- gested, the arteries and trabeculae being relaxed, the elastic tissue yielding, and the organ containing more blood. This has been attributed, by some, to the necessity of having an excessive quantity of blood in the portal system during digestion, the spleen acting as a reservoir, but more probably it is connected with important changes going on in the spleen pulp. Rapid contraction of the spleen takes place when the vagus or splanchnics are stimulated ; it also contracts on galvanising the medulla. Rhythmical contractions of the spleen have been noted in the cat and dog. (2) The spleen is a source of white blood-cor- puscles to the blood, the splenic vein containing 1 white to 60 red, whereas in ordinary blood it is 1 to 232 The Ductless Glands 400. The blood in passing through the pulp conies into close relation with the lymphoid tissue, and new corpuscles are formed. (3) Red corpuscles are probably broken up and disintegrated in the spleen. The spleen pulp shows yellowish granular matter, which may be derived from the haemoglobin of the red corpuscles. This colour- ing matter may be converted into pigment in the liver. (4) The conversion of white corpuscles into red has been attributed to the spleen, but this is very uncertain. (5) The spleen has been successfully removed from a dog, and no great change has been noted in the animal. In some cases the lymphatic glands have become enlarged after the removal of the spleen. The human spleen has been removed successfully. (6) The spleen is apt to be enlarged in some morbid conditions, as anaemia, leucocythagmia, ague, and some fevers. Lymphatic Glands Have already been referred to (p 122). Supra-renals The supra-renals are two small bodies of a somewhat triangular shape which surmount the kidneys. They are about \\ in. in height and \\ in. in width. They weigh 1 to 2 drms. each. Structure.— -They are invested by a fibrous coat which surrounds each organ. On section they arc seen to consist of a cortical portion, forming the greater part of the organ, of firm consistence and yellow colour, and a medullary portion, which is soft and pulpy, and of a brownish black colour. Sttpra-renals 23: The Cortical portion consists of a fibrous stroma, in the meshes of which are cells arranged in columns which radiate from the centre of the gland. Fig. 88. —Vertical seclion of supra-renal body (Eberth). 1, cortical sub- stance ; 2, meduilary substance, a, capsule ; //, zona glomernlosa ; c, zona fasciculata ; d, zona reticularis ; e, groups of medullary cells ; /, section of a vein. 234 The Ductless Glands The cells are granular, yellow, and nucleated, and are about T7 -nro in- in diameter, and contain minute oil- globules. Small arteries run between the columns. The Medullary part is separated from the cor- tical by a layer of connective tissue, and is best marked in the supra-renals of young animals. It consists of a stroma, in the meshes of which are en- closed groups of cells which are coarsely granular, have no oil-globules, and some of them are branched. Nerves. — Bundles of nerves run through the cortex, and form a network in the medullary part. Function. — Nothing is known for certain re- garding the function of these bodies. The most interesting point is their connection with Addison's disease, in which tuberculosis of the supra -renal capsules is accompanied by a bronze tint on the skin, vomiting, and progressive emaciation. Some maintain that, like the spleen, they exercise some in- fluence on the elaboration of nutritive material in the blood. Others believe them to be connected with the nervous system, and the cells of the medullary portion to be nerve-cells. Thyroid Gland The thyroid gland consists of two lateral lobes situated on either side of the trachea and larynx, and joined by an isthmus which crosses in front of the trachea at the third and fourth rings. It is soft, of a reddish colour, and weighs from i to 2 oz., and is larger in the female than in the male. Structure— It is invested by a layer of fibrous tissue which connects it with the surrounding parts. It is composed of a number of closed vesicles, which are from w },u i n - i' 1 diameter to the size of a millet- seed. Each vesicle is surrounded by a plexus of capillaries, and lined by a single layer of epithelium. Thyroid Gland 235 They normally contain a clear yellow viscid fluid, and sometimes white corpuscles and degenerated red cor- puscles. The organ is very vascular, receiving a large blood-supply (fig. 89). Function. — But little is known concerning the function of the thyroid body. It has been supposed that, like the spleen, it pours white corpuscles into the blood. It is enlarged in certain diseases, as in goitre, which is common in Derbyshire and the valleys of Fig. 89. — Section of the thyroid gland of a child (Quain's Anatomy). Two complete vesicles are seen. In the middle of one of the spaces a blood- vessel is seen. Between the epithelium there are small cells like lymph corpuscles. Switzerland, and seems to be connected with the constant use of water impregnated with magnesian limestone ; and in exophthalmic goitre, a disease characterised by enlarged thyroid, prominence of the eyeballs, and irregular action of the heart. Horsley's experiment in removing the thyroid in monkeys and dogs produces tremors of the muscles ; a diminution of the red blood corpuscles, so that profound anaemia results ; the white blood corpuscles are increased in 236 The Ductless Glands number ; the subcutaneous tissues and the salivary glands become distended with mucin. The symptoms produced resemble myxoedema as seen in the human subject. Thymus Gland The thymus gland reaches its full development at the end of the second year of life, and then gradually dwindles away. When examined in an infant it is seen to be an elongated, soft, pinkish body lying behind the sternum, and in front of the pericardium and great vessels : it extends into the neck some distance, being covered in by the sterno-hyoid and thyroid muscles. It consists of a capsule of fibrous tissue, sending trabecular into the gland, dividing it into lobes and lobules. These lobules are again divided into follicles. These follicles are irregular in shape, and contain a central portion or medulla and an external cortical portion. The follicle consists of lymphoid tissue in some respects resembling a solitary gland ; in the cortex there is a retiform network, the meshes being filled with lymphoid cells. In the medulla the retiform tissue is coarser and the cells fewer, but it contains peculiar bodies, known as the concentric corpuscles of Hassall. The thymus is pro- bably a lymph-gland. Nervous System 237 CHAPTER XVIII NERVOUS SYSTEM The nervous system is divided into — 1. The Cerebro-spinal system. 2. The Sympathetic system. 1. The Cerebro-spinal includes the brain, spinal cord, certain ganglia, motor and sensory nerves. The motor nerves are supplied to the striated or voluntary muscles ; the sensory are distributed to the organs of sense, skin, and other parts endowed with sensibility. The nerve-fibres are mostly of the medullated kind. . 2. The Sympathetic consists of a series of gan- glia and nerves, which supply the involuntary mus- cular fibre of the uterus, stomach, intestines, ducts, and blood-vessels. The sympathetic system has a less symmetrical arrangement than the cerebro-spinal ; the nerves are of a reddish colour, and are com- posed, for the most part, of non-medullated or grey fibres. These two sections of the nervous system are intimately connected with each other — indeed, they can hardly be regarded as distinct systems ; the sympathetic may be regarded as that portion of the nervous system which supplies the internal organs and blood-vessels. Structure of the Nervous Mechanism : — I. Purely conducting organs, nerves. II. Terminal end organs. III. Central organs, as brain, cord, ganglia. 2 3 8 Nervous System. I. Nerves The nerves consist of bundles of nerve-fibres bound together by a common tissue sheath. This =3 in v v -=3 ay= «- — 4J OJ 3 (3 E •r; ra oj Jn " oj o ri 43 u .— ' lls-S^ . ,o G *" <"■£ 5-5 > *4i E .2 ■* 4j Si -.b S Si « j;? Or- C ' J3 « S8Eo^. 2E ° p~= " S M °.2 <" g H S ~ c'E-.S— ««- tl cJ ft « « ■ aj'C „, i; ^ '* Si d e- a rt ~ w i e 4) o g j- a; 485 8a .!§ sheath, which is called the epineurium, surrounds the whole nerve and binds its bundles or fasciculi to- gether. It contains blood-vessels, lymphatics, con- Medullated Nerves 239 nective-tissue cells, and adipose tissue. Each nerve- bundle or funiculus is surrounded by a special sheath of its own, termed the perineurium (fig. 90, p). Be- tween the lamella? of the perineurium there are dis- tinct lymph spaces. The nerve-fibres are separated from one another by a delicate connective tissue called the endoneurium, which contains many connec- tive-tissue cells (fig. 90). Nerve-fibres are of two kinds : — (a) Medullated. (b) Non-medullated. (a) The Medullated Nerve-fibres are present, for the most part, in the cerebro-spinal system. They vary very much in size, being from -,o',ro to Tl ,J„ s in. in diameter. When examined shortly after death, they appear as translucent, glistening threads, with a dark border. On addition of various reagents it can be made out that a medullated nerve consists of : (1) Primitive nerve-sheath. (2) Medullary sheath. (3) Axis cylinder. (1) The. primitive nerve-sheath or neurolemma is a thin hyaline membrane which surrounds the nerve- tubule. In this sheath annular constrictions may be seen at intervals, which project into the nerve tubule as far as the axis cylinder ; these constrictions are called the nodes of Ranvier. On the inner surface of the sheath are nuclei surrounded by finely granular protoplasm ; these nuclei do not belong to the neuro- lemma. The neurolemma is absent in the nerves which form the white substance of the brain and cord, optic and acoustic nerves. Many of such nerve- fibres are varicose, owing to small accumulations of fluid between the axis cylinder and medullary sheath. (2) The medullary sheath, or white substance of Schwann, is semi-fluid during life, but coagulates after >40 Nervous System n death. It consists of fatty matters, soluble in ether, which when squeezed out of the primitive sheath appear like bright drops with a dim contour. When c the white substance has coagu- lated, the nerve, which imme- diately after death appears to have a single outline, becomes dark-bordered. According to Klebs, the axis cylinder and medullary sheath are separated by a narrow space, called the periaxial space, containing a cement substance. The me- dullary sheath is stained black by osmic acid. It is absent at the nodes of Ranvier (fig. 91). (3) The Axis cylinder is a narrow thread which runs through the centre of the nerve. It is albuminous in nature, is continuous with the poles of nerve-cells, and stains with car- mine, logwood, chloride of gold, or, better than all, aniline blue- node with nucleus: c, axis bi ac k. In places it can be seen cylinder, projecting at the , ... L . £>.,,., broken end ; /, primitive to be distinctly fibnllated. sheath within which the Medullated nerves when medullary sheath, which is stained dark by osmic acid, coming near their terminations is somewhat refracted. ^ thdr meduUary sheatn . Some medullated nerves, especially in the optic nerve, possess more or less regular varicose enlargements. (<5) Non-medullated Nerves consist of— (1) Primitive nerve-sheath. (2) Axis cylinder. They closely resemble the medullated nerves, but the white substance of Schwann is wanting. They Fig. 91. -Two portions of me dullaled nerve-fibres, afte treatment with osmic acid, showing the axis cylinder and the medullary and pri- mitive sheaths (Quain's Anatomy). A, node of Ran- vier ; b, middle of inter- .Nerve Endings 241 vary in size from ^-^ to - ff J i)tF in. in diameter. They are present for the most part in the nerves of the sympathetic system, but they are also present in the cerebro-spinal nerves. II. Terminal End Organs (A) Sensory Nerves end in — 1. Networks or plexuses. I (a) Pacinian bodies. (b) End bulbs.. (c) Touch corpuscles. (d) Rods and cones,' taste-buds, &c. &c. 2. Special Organs (B) Motor Nerves end in — 1. Non-striated. 2. Striated muscle. 1. Sensory Networks or Plexuses. — The nerve-bundles as they approach their terminations divide and re-divide till the branches consist of only one or two tubules. In the skin and mucous membrane, when the nerves are approaching the surface epithe- lium, they lose their medullary sheath, join together, and form the subepithelial plexus. From this plexus fine fibrils are given off, which, according to Klein, pierce the rete mucosum, and end beneath the cells of the horny layer, or, according to some, in the epithelial cells themselves. In the cornea there are two terminal plexuses, superficial and deep. The superficial forms a sub- epithelial plexus, which gives off minute fibrils, which end in the interstitial substance between the epithelial cells on the surface. The deep plexus is situated in the substance of the cornea ; some of the fine fibrils are said to end in the corneal corpuscles. R 242 Nervous System lip 2. Special Organs— («) Pacinian Bodies are ovoid in shape, about T V to ^ in. in diameter-, and are found attached to the digital, plantar, pudic, infra-orbital nerves, and mesenteric nerves' of cat. These bodies consist of a number of concentric membranes placed inside each other, enclosing a clear space in the centre, which contains the termina- tion of a nerve. Each cap- sule consists of a hyaline membrane marked with fine transverse fibres, and lined on its inner surface by a layer of endothelial cells. There is no fluid between the layers, as sometimes described. The central clear mass contains a hyaline matrix and an axis cylinder, the sheath and white substance of Schwann being lost before the nerve enters the clear space. Besides the nerve a minute artery enters the Pacinian body, and distributes capillaries between the capsules. {b) End Bulbs exist in man in the conjunctiva, lips, mucous membrane of mouth, soft palate, genital organs. They are about ^ m in. in diameter, and consist of an ovoid corpuscle, in which a medul- lated nerve-fibre terminates. They are surrounded by a capsule continuous with the perineurium sur- rounding the nerve. The matrix is a granular mass containing oval nuclei. The nerve loses its medullary sheath and after branching ends in bud-like processes. Fig. 92. — Tactile corpuscle within a papilla of the skin of the hand, stained with chloride of gold (Quain's AnatomyX -£>, epidermis. The convolutions of the nerve-fibres within the cor- puscle are well seen. Nerve Endings 243 (c) Touch Corpuscles or Tactile Corpuscles occur in the papilla; of the corium of the volar side of the hands and feet in man (fig. 92). They are about -^ in. long. They are connected with one or two medullated nerve-fibres. The nerve-fibre winds round the corpuscles several times, then loses its medullary sheath and penetrates into its substance where the axis cylinder divides, is more or less coiled, and ends in slight enlargements, (d) Other end organs, as the a b ii, ," :->- f) i - .y — FlG. 93.— Nerve ending in muscular fibre of a lizard (Quain's Anatomy). In a, the end-plate is seen edgeways ; b, from the surface ; s s, sar- colemma ; pp, expansion of axis cylinder. In b the expansion of the axis cylinder appears as a clear network. rods and cones, taste-buds, organ of Corti, will be described in connection with sight, taste, &c. (B) Terminations in Muscles. — 1. Non-stri- ated muscles are supplied with non-medullated nerves, which form plexuses ; these plexuses give off the primitive fibrils which run in the interstitial substance between the cells ; and, according to some, give off fine branchlets, which enter the nuclei of the cells themselves. 244 Nervous System 2. Striated Muscle. — Nerves surrounded by their perineurium run in the connective tissue forming the sheath of the muscle. Branches are given off which form a plexus ; other branches containing two or three nerve tubules form an intermediate plexus for the supply of the smaller bundles of fibres. The nerve-tubules enter the muscular fibres, the primitive sheath becomes fused with the sarcolemma, while the axis cylinder loses its medullary sheath and passes through the sarcolemma ; the axis cylinder ends on the surface of the muscle substance, becoming im- bedded in a flat granular mass, the end-plate of Kiihne. The end-plates viewed in profile form Doyere's promi- nences (fig. 93). III. Structure of the Central Organs The Grey Matter is present on the surface of the convolutions of the cerebrum, cerebellum, in the Fig. 94. — Two nerve cells from the cortical grey matter .of the cerebellum (Quain's Anatomy). central parts of the spinal cord, corpora striata, optic thalamus, corpora quadrigemina, ganglia, &c. Nerve Cells 245 It consists of : 1. Nerve-cells. 2. Nerve-tubules. 3. Pigment. 4. Blood-vessels. 5. Neuroglia. White Substance consists of : 1. Nerve-tubules. 2. Blood-vessels. 3. Neuroglia. 1. Nerve Cells are small rounded or branched bodies, destitute of a cell wall, formed of finely granu- lar, protoplasm, in reality consisting of a fine network of fibrils. Each cell contains a nucleus, having a well-defined capsule, fine network, and a nucleolus. They sometimes contain pigment. The cells are surrounded by a perivascular space. In shape they are apolar, unipolar, bipolar, or multipolar, according to the number of processes they possess. Each process is continuous with the axis cylinder of a nerve (fig. 94). Neuroglia. — This name is given to the frame- work of the grey and white matter of the cerebrum, cerebellum, and cord. It con- sists of branching nucleated cells (glia-cells), the branches passing between and supporting the nerve * fibres and cells. In some parts of the nervous centres, as, for instance, the grey matter of the cerebrum, the supporting sub- stance appears to be finely granu- lar, but this appearance is really fig. 55. -Part of resi- due to the fine nerve tubules and $£?*■ °Anatlmy). cord branches of the glia-cells seen in section. The glia-cells are fibrillated, the fine fibres passing through the body of the cell. The White Matter is distributed in various places in the brain and cord, connecting the grey matter of different parts. The nerve-fibres are medullated, but have no primitive sheath. They vary in size, often possess varicose swellings, due to an accumulation of fluid between axis cylinder and medullary sheath. 246 Nervous System Ganglia. — These consist of rounded or elongated bodies found in various situations in connection with nerves. They are present in the following places : — 1. Cerebro-spinal. — On the posterior roots of the spinal nerves ; on the roots of the fifth (Gasserian), Nerve Ganglia >47 facial, vagus, glossopharyngeal ; in several other situa- tions, as the ophthalmic, Meckel's, the otic, and sub- FlG. 97. — Two nerve-cells from a spinal ^an^lion (Quain's Anatomy), s/t, nucleated sheath : «, «, nuclei of primitive nerve-sheath. From each cell a nerve-fibre arises, and after a convoluted course bifurcates opposite d, from which point they separate. maxillary. These ganglia are surrounded by a fibrous sheath continuous with the nerve with which they are 248 Nervous System connected ; from this sheath prolongations are sent into the substance of the ganglion. On examining a section under a low power (fig. 96) the nerve-fibres will be seen entering the ganglion at a, and leaving at b, the principal mass of nerve-cells being present at the periphery, but they are also present in the more central parts. Some nerve-fibres apparently pass through the ganglion without being connected with any cell ; all the cells are, however, connected with a nerve-fibre. The cells differ in size, are unipolar, Fig. c -A group of ganglion cells interposed in a bundle of sympathetic nerve-fibres from the bladder of a rabbit (Klein). rounded or pyriform in shape, are surrounded by a sheath, and have a large oval nucleus and nucleoli. The single nerve-fibre with which they are con- nected (fig. 97) divides after leaving the cell, and passes in opposite directions. This bifurcation is often T-shaped. The nerve-cells are not all unipolar in the cerebro-spinal ganglia, for in the otic, spheno- palatine, submaxillary, and ophthalmic there are multi- polar cells. 2. Sympathetic. — There are numerous ganglia in connection with the sympathetic system, some of which, as the semilunar, are of considerable size ; others, as those situated in the walls of the bladder Functions of Nerves 249 or heart, are microscopic. The principal set are (a) forming a chain by the side of the vertebral column ; (b) in numerous places in the walls of the heart, intestines, uterus, and in connection with the plexus. In these ganglia the cells may be unipolar, bipolar, or multipolar. They are mostly oval or pyriform, with a sheath, nucleus, nucleoli (see fig. 98). PROPERTIES AND FUNCTIONS OF NERVES. i. Nutrition. — Nervous matter receives a rich supply of blood ; the network of capillaries in the grey matter is closer than in the white. The nerve- cells receive their nourishment from liq. sanguinis which has exuded from the vessels. Active nerve- cells absorb O and eliminate C0 2 . Some nerve- centres exercise an important influence over the nutri- tion of certain nerves ; thus, if a motor nerve of the spinal cord is cut off from the grey matter in the anterior cornua, it undergoes fatty degeneration, and the muscle it supplies becomes atrophic. If a sensory nerve is divided, the part attached to the posterior ganglion remains normal ; that part which has been separated from the ganglion degenerates. When a nerve is cut in a mammal, the ends often reunite in a few weeks. 2. Nervous Excitability and Conductivity. Nerves, like muscles, are irritable or excitable. If one end of a nerve is irritated by the application of a stimulus, such as the application of heat, the. elec- trodes of a battery, or by other means, the irritation or excitation is conveyed along the nerve to its farthest extremity. If the nerve is attached to muscular fibre, a contraction is produced ; if the nerve ends in a sensory centre, a sensation is produced, or the secre- tion of a gland poured out if the nerve terminates there. The nerves receive impressions through the medium of certain terminal organs, as the touch 250 Nervous System corpuscles, rods and cones of retina, and convey the impression produced to a certain sensory centre, and a sensation is felt ; or they receive an impulse from certain motor centres, and convey the impulse to their termination in the end plates of the muscles, and a muscular contraction ensues. If the nerves are too frequently excited they become fatigued, and a certain amount of repose is necessary for them again to conduct impressions. There are several methods of measuring the velocity of the nerve-current. The ordinary method in motor nerves of frog consists in applying the elec- trodes directly to the muscle, and measuring the time that elapses before the contraction, the contracting muscle recording its movements by means of a lever on a revolving drum (a chronograph marking time) ; then, if the electrodes be applied to the nerve, at some distance from the muscle, and the time again measured, it is evident that the difference between the two will be the time that the nerve-current took to travel through the nerve (fig. 31). The velocity of the nerve current has been cal- culated to be about 80 ft. per sec. in the frog, and 100 to 120 ft. per sec. in man, though some have placed it at 200. Sensory impressions in man have been mea- sured in the following way : — Arrangements are made for a person to give a signal — the moment he feels a prick, say, on his great toe — and the time noted between the prick being administered and the signal given. Another experiment is made in the same way by pricking a point nearer the brain, say the knee, and the time measured. The difference between the two will be the time the impression takes to travel from toe to knee. It has been found that the velocity is about the same as in motor nerves — no to 120 ft. per sec. This method is, however, open to many objections. Electrotonus 2 5 1 3. Electrical Phenomena of Nerves. — Elec- trical currents are present in living nerves. If a piece of a'nerve be cut out and placed upon the elec- trodes of a galvanometer, so that the surface of the nerve touches one electrode and the cut end the other, a current will be observed to pass from the surface through the galvanometer to the cut end. The nerve-currents exactly resemble the muscle- currents. When the nerve is excited there is a dimi- nution or negative variation of the normal current. Electrotonus. — If a constant current be passed along a nerve, the nerve is thrown into a peculiar state termed electrotonus. If the current travel in the direction of the natural nerve-current, the latter is increased ; if in the contrary direction, it is diminished. While a portion of nerve is traversed by the con- stant current, its properties are to some extent altered ; the portion in the neighbourhood of the positive pole is said to be in an aneledrotonic state, while the portion of nerve in the neighbourhood of the negative is in a cathelectrotonic state. The position of the neutral point between the two varies with the strength of the current passing through the nerve. With a current of medium intensity, the neutral point is midway between the poles ; with a weak current the neutral point is nearer to the positive than the nega- tive ; with a strong current the neutral point is nearer the negative than the positive. When a nerve is in theanelectrotonic state, its natural nerve-currents are increased, but its excitability and conductivity are diminished ; when in the cathelectrotonic, its natural nerve-current is diminished, but its conductivity and excitability are increased. Pfliiger's Law of Contraction. — When a constant current of medium strength is passed along a motor nerve, no effect is produced upon the muscle, except on opening and closing the current. The con- 252 Nervous System traction of the muscle is influenced (1) by the direction, (2) by the strength of the current— that is, the strength of the contraction on making and breaking contact varies not only according to the strength of the current applied, but also to the direction, whether the current is passed downwards in a direction from the spinal cord to the muscle, or in an upward direction from the muscle to the cord. The following is a brief statement of the facts : — Stren -;Lh of Current Descending 1 Make ! Break Ascending Make Break Very weak Weak . Medium Strong . '. '. '. 1 C R C R C C C R R— rest. R C C R R R c C C — contraction From this' table it will be seen that, if either a weak or a strong current is passed along a motor nerve in a downward direction, there will be a contraction at making only. With a strong ascending current there is a contraction on breaking only. Pfltiger's law also holds good in the case of man, but the conditions are necessarily different as the elec- trodes are applied to the skin and not directly to the nerves or muscles. It is usual to apply one electrode over a nerve or muscle and another at some distant part, as for instance, the back of the neck. If the anode (4) be placed at the back of the neck, and the kathode ( — ) over the nerve, the current will be a de- scending one, and vice versa. When the kathode is applied over the nerve and the current is closed or opened, we have a kathodic closure contraction or a Functions of Nerves 2 53 kathodic opening contraction ; if the anode is applied, we have an anodal closure or opening contraction. The following formula expresses what occurs normally. Weak currents Medium currents . Strong currents KCC KCC KCC nil ACC ACC nil AOC AOC nil . nil ICOC In disease, as for instance in ' infantile paralysis,' this does not hold good, there being what is termed the reaction of degeneration ; normally KCC appears with a weaker current than ACC, but in the reaction of degeneration ACC appears with a weaker current than KCC, or with a current of the same strength. Moreover, excitability to the induced current is diminished or lost, the excitability to the continuous current being exaggerated. Functions and Classification of Nerves Nerves may be divided into : I. Efferent or Cen- trifugal nerves Motor, supplying the vo- luntary or involuntary muscles. Vaso-motor, supplying the muscular fibres of the blood-vessels. < 3 Secretory, supplying glan- dular epithelium. Inhibitory, which modify the action of nerve- centres. 5. Trophic, regulating the nutrition of a part. 254 Nervous System 1 1. Nerves of common sensa- tion, pain, touch, &c. II. Afferent or Cen-12. Nerves of special sense. tripetal nerves. 1 3. Nerves taking part in re- flex actions, and which cause no sensation. /i. Connecting motor cen- III. Intercentral J tres. nerves. ] 2. Connecting sensory cen- ( tres. 1. Motor Nerves. — Each muscle in the body has its ' nerve-supply,' or its nerve which connects it with motor centres on the surface of the brain. Stimulation of the nerve evokes a muscular contrac- tion. 2. Vaso-motor Nerves. — These nerves are divided into vaso-constrictor and vaso-dilator or vaso-inhibitory. Stimulation of the cervical sympa- thetic produces contraction of the arteries supplied to the ear and face ; stimulation of the splanchnic, con- traction of the arteries of the kidneys : such nerves are vaso-constrictor. Stimulation of the chorda- tympani causes dilatation of the vessels supplied to the submaxillary gland ; stimulation of the nerves supplied to the arteries of corpora cavernosa causes dilatation of the vessels and turgescence of the erectile tissue : such nerves are termed vaso-dilator or vaso- inhibitory. 3. Secretory Nerves. — The chorda-tympani is not only a vaso-inhibitory nerve, but also contains fibres which stimulate the glandular epithelium of the submaxillary gland. There are also nerves supplying the mammary and lachrymal glands, which, when stimulated, increase secretion. 4. Inhibitory Nerves. -Certain centres in the brain or medulla exercise a depressing or hindering Functions of Nerves 255 action on other centres. The nerves which connect these centres are called inhibitory nerves. The brain exercises an inhibiting or controlling influence over the centres for defaecation and micturition in the cord. The vagus is the inhibitory nerve supplying the heart, inasmuch as when stimulated the heart's action is slowed, and if the stimulus be sufficiently strong it stops in diastole. 5. Trophic Nerves. — The nutrition of the body is dependent, to a certain extent, upon the ner- vous system. Thus in certain diseases of the spinal cord bedsores very rapidly form over the sacrum. While the nervous system exercises an important influence over the nutrition of a part, it is doubtful if there are any nerves whose sole office consists in regulating the nutrition of a part. Sensory Nerves. — Many divisions may be made in this group. Thus there are nerves which convey sensations of pain, touch, temperature, or of special sense. The nerves of common sensation con- nected with the spinal cord pass through the posterior roots, and have a ganglion situated in their course, just outside the cord. If sensory nerves are divided, a sensation of pain is experienced if the central end is irritated. Eccentric Reference of Sensations. — The mind refers the origin of every sensation that reaches it through a sensory fibre to the end organ of that fibre, even though stimulation has been applied to the trunk of the nerve. Thus, persons whose arms or legs have been amputated often feel sensations which they refer to their ringers or toes. Any stimulation of the optic nerve, mechanical or electrical, the mind refers to the action of light upon the retina. Functions of Terminal Organs. — Probably all nerves end at their peripheral distribution in some form of terminal organ. The optic nerves are 256 Nervous System connected with the rods and cones of the retina, and other sensory nerves are connected with taste-bulbs, olfactory corpuscles, tactile corpuscles, or epithelium. Motor nerves end in end-plates inside the sarcolemma. Light will not affect the optic nerves, except through the medium of the rods and cones ; sensations of touch will not be received at the brain if the skin is stripped off the fingers. The terminal organs seem to play the part of receivers of impressions, and awaken an excitation in the nerves connected with them. Functions of Nerve Centres Groups of nerve-cells, which form the nerve- centres, are arranged in the body in two systems, the cerebro-spinal and the sympathetic system consisting of ganglia scattered through the body. The centres may be classified in various ways, according to their functions ; thus, on the sur- face of the brain there are motor or discharging centres, centres of special sensations, as of sight touch ; in the Fig. go.-Di ag ra m n-presejiting a medulla there are inhibitory simple reflex act. M, muscle ; Nc, -* nerve-centre ; s, sensory surface, and accelerating Centres. They all, however, fall into two great divisions, though it is not always easy to say to which class they belong ; these are automatic centres and reflex centres. Automatic Actions are actions which are evoked in the absence of any influence external to the nerve-centre. The brain is the seat of the higher automatic centres, those connected with volition and intelligence. In the medulla the respiratory centres, cardiac centres, vaso-motor centres, are in a certain sense automatic. So are also the intrinsic ganglia of Reflex Actions 257 the heart, and the small ganglia found in the walls of the intestines. At the same time it must be remem- bered that many of the centres enumerated above are influenced by sensory or -afferent impulses, and are reflex as well as automatic ; indeed, some would deny them their automatism, and believe that no motor im- ' pulses can be generated in the absence of all eccentric influences. Reflex Actions. — For reflex action the follow- ing apparatus is required : — 1. A sensory surface in connection with an afferent nerve (fig 99, s). 2. A nerve-centre, nc. 3. An efferent nerve connected at its central end with the nerve-centre and by its peripheral end with some muscle, or muscular tissue, or gland, m. The sentient surface or end organ being excited, the im- pulse travels along the afferent nerve to the centre, and is reflected from the centre along the efferent nerve to the muscle. The stimulus may be of various kinds ; it may be a simple tickling of the skin, or a bright light, or a hair in the glottis. Some reflex actions are performed without one being conscious of them, as the contrac- tion of the pupil or the changes in the calibre of the arteries. Others, as winking or swallowing, are attended with consciousness. Some reflex acts can be influenced or controlled by the will, as micturition or coughing ; others are entirely beyond the control of the will, as the second and third acts of swallowing. The excitability of the centres in the cord is increased by severance from the centres in the brain. Thus, reflex movements are more active during sleep, or in a decapitated frog than in an uninjured one. Strychnine in toxic doses increases the irritability of the centres of the cord, while the bromides, chloral, and atropine diminish their excitability. 258 Nervous System In some cases the reflex act seems to be adapted to a purpose, as in the efforts made by a decapitated frog to wipe away a drop of acetic acid placed on its back. The following instances of reflex acts may be taken as examples : — (1) Contraction of iris : aff. nerv., the optic ; ' nerv. centr., the corpora quadrigemina ; eff. nerv., third. (2) Winking : aff. nerv., the fifth or optic ; nerv. centr., the corpora quadrigemina ; eff. nerv., seventh. (3) The first respiration after birth from impres- sion of cold on the skin ; aff. nerv., the sensory of skin ; nerv. centr., the medulla ; eff. nerv., phrenics, intercostals, &c. (4) Vomiting from tickling fauces : aff. nerv., the glossopharyngeal, fifth ; nerv. centr., the medulla ; eff. nerve., phrenics, nerves to abdominal muscles, vagi. (5) Sneezing from a draught of cold air; aff. nerv., the nasal branches of fifth ; nerv. centr., the medulla ; eff. nerv., intercostals, nerves to abdominal muscles, phrenics, &c. (6) The secretion of saliva is a good example of a reflex act in which a more complicated mechanism is brought into action than in some of the examples given. In the secretion of saliva from the submax- illary salivary gland, the afferent nerves capable of stimulating the nerve-centre are as follows : — (1) The nerves of taste ; (2) the sensory branches of the fifth nerve supplied to the mucous membrane of the mouth ; (3) nerves of smell ; (4) optic nerves ; (5) gastric branches of the vagus. The nerve-centre for secretion of saliva is situated in the medulla. The efferent nerve is the chorda tympani, which contains two sets of fibres, vaso-dilato and secretory fibres ; Spinal Cord 259 so that when this nerve is stimulated reflexly or directly, the artery supplying the gland dilates and the cells of the gland are stimulated to secrete (fig. 100). Reflex actions are also seen in various forms of disease or abnormal conditions, such as vomiting ARTERY CLAND VASO -DILATOR NERVE SECRETING/ NER¥E NERVE/ CENTRE & M £MBRAA/E Fig. 100. — Diagram illustrating innervation of salivary glands. from cerebral tumour, vomiting of pregnancy, grind- ing of teeth from irritation of worms, palpitation of heart, &c. Time occupied in Reflex Acts.— The rapidity with which a reflex act is performed varies from -05- •06 second. The stronger the stimulus applied the shorter will be the time.' Spinal Cord The spinal cord has its upper limit at the margin of the occipital foramen, and extends downwards to the lower border of the first lumbar vertebra. It is fifteen to eighteen inches in length, and presents s 2 26o Nervous System two enlargements, the cervical arid lumbar. It ends below in the cauda equina, which consists of a bundle of nervous cords. Structure. — The cord consists of — i. The grey matter in the centre. 2. The white substance externally. 1. The Grey Matter appears in the form of two irregularly shaped crescents, joined to one another by a commissure, in the centre of which is the central Post, median col. Post, septum Post. ex. col. Am. cornu . Cells of ant. cornu Central canal^ Subs, gelat. cart.'' Post, commissure'/ Ant. commissure Ant. root Ant. fissure Direct pyramidal tract Fig. ioi. — Diagrammatic transverse section of the spinal cord x 6, on z level with the eighth thoracic nerve (after Schwalbe). canal. The anterior cornu (fig. ioi), or horn of the crescents, is broad and rounded ; the posterior cornu is long and narrow, tapering towards Spinal Cord 261 the external surface of the cord at the post. lat. fissure ; near its tip it has a pe- culiar semi-transpa- rent appearance — the substantia gelatinosa. Near the outer sur- face of each crescent the grey matter is less sharply marked off from the white than elsewhere, its pro- longations forming a sort of network — the processus reticu- laris. A somewhat pointed projection of the grey matter in the lateral region is called the inter -me- diolateral cornu. The grey crescents vary in shape in different parts of the cord, being narrow in the dorsal ; the ant. cor- nua are large and broad in the cervical and lumbar regions. The nerve-cells of the grey matter are collected into four groups or rather co- lumnar tracts. (1) Vesicular column of the anterior cornu Fig._ ioia. — Diagrammatic sections of the spinal cord at different parts to show the chief localised tracts of fibres in the white substance (Quain's Anatomy). /., at the level of the sixth cervical nerve ; //., of the third dorsal; ///., of the sixth dorsal ; IV., of the twelfth dorsal ; V. t of the fourth lumbar, d.p.t., direct pyramidal tract ; cj.t., crossed or lateral tract ; c.t., direct cerebellar tract ;g, post- median column. 262 Nervous System (fig. 1 01). These, for the most part, are multipolar, and vary in size from ^ Tl - in. to ¥ 'n, in. They are directly connected by their processes with the motor nerve- fibres. They are best marked in the cervical and lumbar enlargements. (2) Posterior vesicular column of Clarke (fig. 101), reaches from the eighth cervical to the third lumbar. The cells are large and fusiform, with their long axes parallel to the cord. (3) Column of the intermedio-lateral tract, ox posterior -lateral group (fig. ior), confined to the dorsal and upper part of the lumbar spinal cord Consists of small spindle-shaped cells. (4) A small group of multipolar cells in the posterior horn. 2. The White Matter is divided into two halves by the anterior median and posterior fissures. Each lateral half is again divided by two lateral fissures, which are merely grooves along the line of attachment of the anterior and posterior branches into an anterior, lateral, and posterior column. The white substance on section, and examination by a high power, displays the cut ends of the nerve-fibres, presenting small rings with a dot in the centre, the dot representing the axis cylinder, and the surrounding space the white sub- stance of Schwann. Course of the Nerve-Fibres in the Cord.— By various methods of research, especially by study- ing the development of the cord, and certain patho- logical changes occurring as the result of injury or disease, the following tracts have been distinguished in the cord : — A. Descending Tracts.— (1) The direct pyra- midal tract, or column of Turk (fig. ioia, d.p.t.), is traced down from the anterior pyramid of the medulla of the same side, and, therefore, has not decussated. Probably the decussation of these fibres goes on along their whole course. This tract cannot be traced farther than the middle of the dorsal region. Spinal Nerves 263 (2) Tlie lateral or crossed pyramidal tract (c.p.t.) can be traced down, diminishing as it goes, to the third or fourth sacral nerves. B. Ascending Tracts. — (1.) The direct lateral cerebellar tract (c.t.) lies between the lat. pyramid, tract and the outer surface of the cord. It disappears at the second or third lumbar nerves. The remainder of the antero-lateral column has not been mapped out ; it is, probably, commissural. (2) The post-median column, or tract of Goll (g), only extends downwards as far as the middle of the dorsal region. (3) The posterior external column is sometimes called Burdock's column, ox fasciculus cuneatus. (4) The anterior division of the lateral column is called the anterior radicular zone. Spinal Nerves. —Thirty-two pair of nerves arise from the cord, each nerve arising by two roots, an anterior and a posterior. The anterior arises by several bundles from the antero-lateral region of the cord ; the posterior arises by a single bundle from the posterior horn of grey matter. The two roots join to form the trunk of the spinal nerve ; there is a ganglion on the posterior.root. Section of the anterior root is followed by paralysis of the muscles supplied by the nerve ; excitation of the peripheral end gives rise to contraction of the muscles ; irritation of central end has no effect. Section of the posterior root causes loss of sensation in the area of its distribution ; stimula- tion of its central end causes cries of pain ; stimula- tion of its peripheral end has no effect. If the anterior root be divided, the whole peripheral part of the fibres degenerates, so that in a section of a mixed nerve the degenerated motor fibres can be identified. If the posterior root be divided between the ganglion and its junction with the anterior root, all the sen- sory fibres in the mixed nerve below the junction 264 Nervous System degenerate. If it be divided between the ganglion and the cord, the sensory fibres in the mixed nerve remain intact, but the central parts of the fibres degenerate up through the cord to the medulla. Functions of the Cord 1. As a conductor of impressions and impulses. 2. As a series of nerve-centres. The Cord as a Conductor 1. The spinal cord forms a channel of communi- cation between the brain and nerves, passing to the periphery of the body. The exact path of the motor and sensory nerves is not satisfactorily settled, as there are discrepancies between the results obtained by different observers. Motor Path. — Motor impulses travelling from the brain to the anterior pyramid of the medulla, for the most part decussate in the medulla, crossing to the crossed pyramidal tract of the opposite side. A minor portion travelling along the direct pyramidal tract (fig. ioia, d.p.t.) decussates by crossing to the crossed pyramidal tract in the cervical and upper dorsal region. Decussation is therefore going on not only in the medulla but in the cervical and upper dorsal regions. The motor impulses pass from the lateral columns into the anterior cornua, become con- nected with the ganglionic cells there, and leave the cord by the anterior root. Sensory Path. — According to the experiments of Gotch and Horsley, the fibres of the posterior roots divide into two classes ; first, those that run straight up the cord in the posterior column without becoming connected with any nerve-cells until they reach the Functions of the Cord 265 medulla ; and secondly, those which enter the grey substance and become connected nerve-cells. 80 per cent, of the afferent impulses travel up the same side of the cord ; this 80 per cent, is made up of 60 per cent, which travel up the posterior column and 20 per cent, up the lateral column ; while of the remainder some 1 5 per cent, pass up the posterior column on the opposite side, and a few up the lateral column of that side. Reflex Functions of the Spinal Cord 2. Frog. — If the spinal cord of a frog be divided immediately below the occipital foramen, the frog will retain its usual sitting attitude, with the ex- ception of sinking down into a somewhat less erect position, the fore limbs being more spread out. It will exhibit no respiratory movements. If one of the hind legs be pulled out straight and let go, it will be drawn up again to its normal position. If the skin of one flank be tickled, the muscle beneath will contract. Pinch the same spot, or apply a drop of acetic acid, and the leg of the same side will make a sweeping movement to clear away the source of irritation ; if the leg of the same side be held or cut off, the leg of the other side will repeat the movement. Place the frog on its back, it will make no effort to regain its position. The above actions of the brainless frog are complicated, co-ordinated, purposeful in character ; but, however stimulated, the animal never leaps. In the Mammal.— For some days after the division of the cord in a dog, very feeble reactions are given by the nervous mechanism of the cord. After some weeks movements of a varied character are evoked by tickling or pinching the toes. In man, when the cord is crushed from the effects 266 Nervous System of accident or disease, the legs will start up on tickling the soles or in passing water. In the normal condition it is generally possible to evoke reflex actions of the cord by gentle stimulation of the skin by a touch or light stroke. Tickling the soles of the feet, more particularly during sleep, will cause a slight with- drawing movement of the muscles of the foot, called the ' plantar reflex,' The centre for this movement is situated in the lower part of the lumbar enlarge- ment. Irritation of the skin of the buttock will often produce a contraction of the glutei (gluteal reflex), the nerve-centre being situated at the origin of the fourth or fifth lumbar nerves. Irritation of the inner side of the thigh will cause a contraction of the cremaster (cremasteric reflex), drawing up the testicles, the centre being connected with the first and second lumbar nerves. There is also an abdominal reflex and an epigastric reflex, which may be produced by stroking the side of the abdomen and side of the chest respectively. The ' patellar reflex ' is obtained by allowing the knee to swing freely, and then sharply tapping the patellar tendon, the leg jerking forward ; this movement is probably due to the stimulus reaching the muscle direct, the time occupied being too short for a reflex act, but it nevertheless depends upon the integrity of a nerve-centre situated in the upper part of the lumbar enlargement of the cord. Action of Strychnia.-— Strychnia gives rise to an excessive excitability of the spinal cord. An animal having received a poisonous dose, dies in a condition of tetanus, the movements of respiration being arrested. A frog does not readily die, as respiration is con- tinued through the skin ; the Contracted condition of the muscles is abolished by destroying the spinal cord. In a frog under the influence of strychnine, the slightest stimulus of the skin evokes a reflex action, Centres in the Cord 267 the slightest touch sending all the muscles into pro- longed tetanus. Inhibition of Reflex Actions. — The brain exercises a powerful influence in restraining or in- hibiting reflex actions. A brainless frog exhibits re- flex actions better than one with brain intact. If the experiment be tried of suspending a frog with cerebral hemispheres only removed, with its toes dipping in dilute acid, and the time which elapses before their withdrawal noticed, and the same experiment repeated, stimulating the optic lobes at the same time, the time elapsing before the withdrawal will be prolonged, showing the optic lobes have inhibited the reflex centres. Man, by an effort of will, can prevent the with- drawal of his feet if the soles are tickled. Special Centres in the Spinal Cord. 1. Centre for maintaining tonus of the muscles. 2. Centre for sphincter of bladder. 3. Centre for sphincter of rectum. 4. Centre for contractions of uterus. 5. Centre for erection of genital organs. 6. Cilio-spinal centre. 1. The muscles of the body are kept in a constant state of contraction or tonus ; this effect is due, pro- bably, not to an automatic but to a reflex mechanism constantly in action. 2, 3. The centres for micturition and defsecation appear to exist in the lumbar region of the spinal cord. 4, 5. The centres that govern the movements of the uterus and erectile tissues are situated in the lumbar region of the cord. The above centres are to be considered reflex rather than automatic. 268 Nervous System The Medulla Oblongata The medulla is bounded above by the lower border of the pons Varolii, and is continuous below with the spinal cord at a level with the foramen magnum. Structure. — The medulla is divided on the surface by fissures into short columns, which have received Hypoglossal n. N. gracilis N. cuneatus F. solitanus F. gracilis i F. cuneatus Restiform body Subs, gelat. R. Root of 5th Fig. 102. — Diagrammatic transverse section of the spinal bulb at about the middle of the olivary body, to illustrate the principal nuclei and tracts at that level ; x 3 (after Schwalbe). different names ; each lateral half having from before backwards : — Anterior pyramid. Lateral tract and olivary body. Restiform body. fasciculus cuneatus. Posterior pyramid fasciculus gracilis. Medulla 269 White Matter of the Medulla.— The ana- tomical connections of the cord and medulla are very complicated. Tracing upwards the different columns of the cord, we find that the anterior column {direct pyramidal tract) is continuous with the anterior pyramid of the same side. The lateral pyramidal tract joins the anterior pyramid of the opposite side. These two are motor, and are continuous with the crusta. The antero-lateral (exclusive of the above) is sen- sory, and probably passes up through the front part of the restiform body, through the superior peduncles of the cerebellum to the superior lobe. The direct cerebellar tract joins the restiform body and forms the inferior peduncles of the cerebellum. The posterior medium column joins the posterior pyramid and ends in the nucleus gracilis ; fas. posterior external column is continuous with the funiculus cuneatus and ends in the nucleus cuneatus. It is probable that both the latter are continuous with the tegmentum of the opposite side, decussating above the pyramids. Grey Substance of the Medulla. — The medulla contains various nuclei ; two masses of grey matter which 1 receive the posterior pyramids and funiculus cuneatus, called the nucleus gracilis and nucleus cuneatus. The anterior lateral nucleus. The lower part of the fourth ventricle contains the nuclei of the hypo-glossal, spinal accessory, vagus, glosso- pharyngeal, and auditory (figs. 102 and 118). Functions of the Medulla (a) Conductor of impulses and impressions. (b) As a collection of nerve-centres. 270 Nervous System (a) The Medulla as a Conductor The Motor impulses travel through the anterior pyramids, decussating to the lateral column of the opposite side of the cord. The Sensory path is not so well known, it pro- bably passes along the posterior pyramids, decussating above the anterior pyramids (see above). (6) Nerve Centres in the Medulla (1) Respiratory centres. (2) Vaso-motor centre. (3) Cardiac centres. (4) Centres for deglutition. (5) Centre for voice. (6) Centre for mastication (7) Centre for expression. (8) Centre for salivary secretion. (1) The Respiratory Centres consist of an inspiratory and expiratory centre, and are both reflex and automatic. Ordinary respiration is a reflex act ; a venous condition, i.e. a want of O in the blood circulating through the capillaries of the lungs, stimu- lates the terminal fibres of the vagus, ths vagus trans- mits the impression to the medulla, it is reflected along the phrenics, intercostals, &c, to the muscles of inspiration, and a fresh supply of air is drawn into the lungs. The more venous the blood the more vigor- ously are the terminal fibres of the vagus excited, and the more muscles brought into play. If the vagi are divided the number of respirations sink to at least one -third, but they are still continued, and the animal does not die of asphyxia. It is probable that the venous blood supplied to the medullary centre itself Pons 2 7 1 excites it, or, like the intracardiac ganglia, it acts in an automatic manner. (2) The Vaso-motor Centre is the centre of the sympathetic system supplied to the muscular fibre of the blood-vessels, intestines, ducts, &c. If stimu- lated, the vessels all over the body contract, and the arterial tension is raised ; if paralysed or inhibited they dilate, and arterial tension is lowered. The vaso- motor centre keeps the blood-vessels of the body in a state of tonic contraction ; it acts reflexly, and any influence which inhibits it will dilate the vessels. (3) Cardiac Centres. — The rhythmical con- traction of the heart is caused by the action of its own intrinsic ganglia, but its action is regulated by ganglia situated in the medulla. There are two extracardiac ganglia, one accelerating, acting on the heart through the sympathetic, and the other inhibitory, associated with the vagus. (See page 102 and fig. 49). The presence of the above ganglia renders the medulla of vital importance to the living mammal. Death immediately results by destroying it. This can readily be accomplished by ' pithing,' i.e. by thrusting an awl-shaped instrument into the medulla, passing it between the occiput and atlas, and breaking up the nervous substance. Pons Varolii The pons shows, on traverse section, (1) super- ficial and deep transverse fibres, derived from middle peduncles of cerebellum ; (2) longitudinal fibres con- tinuous with the ant. pyramids ; (3) longitudinal fibres of the formatio reticularis, continuous with the medulla ; (4) grey matter of upper part of 4th ven- tricle, containing nuclei of facial, motor of 5th, sensory of 5th, auditory, and 6th. 272 Nervous System Mesencephalon The mesencephalon is developed from the middle vesicle of the brain, and represents the optic lobes of fishes and birds. It includes the nuclei around the aqueductus Sylvii, the corpora quadrigemina, and crura cerebri. The Aqueduct of Sylvius (fig. 103) is a closed canal between the third and fourth ventricles. The grey matter of the aqueduct is a prolongation from the floor of the fourth ventricle, and contains the nuclei of the third and fourth nerves, and the upper nucleus of the fifth. The tegmenta lie below the aqueduct. The Corpora Quadrigemina (fig. 103) are four rounded eminences seated over the aqueductus Sylvii. Each of these bodies is covered with a layer of white matter ; the lower or posterior pair contain a grey nucleus, and are separated by a band of white matter, the fillet, from the grey matter of the aqueduct. The superior pair have a layer of grey matter beneath the white layer on the surface, and underneath the former is a longitudinal tract of fibres— the stratum opticum. The Crura Cerebri (fig. 103) lie beneath the corpora quadrigemina, and consist of (1) an inferior or anterior layer of longitudinal fibres called the erusta (cr.), and is a direct prolongation upwards of the pyramid bundles of the pons, and passes upwards to the internal capsule ; (2) the substantia nigra consists of a centre nucleus of grey matter, the cells containing pigment (s.n.) ; (3) superior or posterior longitudinal bundles of nerve-fibres, interspersed with transverse ones, called the tegmenta (t) ; it is a direct prolongation upwards of fibres probably derived from Cerebellum 273 the posterior columns of the cord (see p. 269), and passes upwards to the optic thalamus. The Cerebellum is situated at the posterior part of the brain, and consists of peduncles, various lobes and processes. The peduncles are three in number, the superior, middle, and inferior ; they serve to con- nect the cerebellum with the cerebrum, pons, and medulla respectively. The cortical portion consists of grey matter, and the central portion of white substance with a nucleus of grey, the corpus dentatum. Fig. 103.- Outline of Lwo sections across the mesencephalon (Quain's Anatomy). A, through the middle of the inferior corpora quadrigemina ; B, through the middle of the superior corpora quadrigemina. cr. t crusta ; s.fi., substantia nigra ; t, tegmentum ; s, Sylvian aqueduct, and surround- ing grey matter; s.c.p., superior cerebellar peduncle; J", fillet, c.q., corpora quadrigemina ; ///. 3rd nerve ; d. V., descending root 5th nerve. The cortical substance has three layers (fig. 104) — (1) External. — Consists of small cells sparingly distributed, some rounded, others irregular in shape, with various processes ; fibres which are for the most part^processes of the large cells of the middle layer, and run at right angles to the surface (b\ (2) Middle. — Consists of cells of Purkinje ar- ranged in a single layer. They are pyriform in shape, nucleated, and have long processes running into the external layer, and are -sT^th to nrWrh inch in dia- meter (c). (3) Inner or granule layer. — Consists of small round granular corpuscles, about the size of white 274 Nervous System Fl ^'=i.^ 4 '7 S '? CU ' r , e , 0f corte '; of cereM1 ™ (Quain's Anatomy), a, pia ™ f, ,'. ?' ?"f nal k , y " ; f > la 5' er of corpuscles of Pmkinie ; rf, inner or granule layer ; ?, medullary' centre. Functions of Brain - 275 blood-corpuscles, arranged in dense masses, which in stained specimens form a well-marked coloured layer (d). Phenomena Exhibited after Removal of Cerebral Hemispheres Frog. — After the removal of the cerebral hemi- spheres the animal main-tains its normal attitude. If laid on its back, it will turn over and regain its feet. Voluntary motion lost. Cannot direct movements. Cannot jump. Cannot recover position if-{ aid on back. Olfactory lobes. Cerebral lobes. Fig. 105. — Diagram illustrating higher nerve-centres of the frog (after Lauder Brunton). If its foot is pinched, it will hop away. If thrown into water, it will swim, reach the edge, clamber up, and sit perfectly still. If its back is stroked, it will croak. If placed in water and the temperature raised, it will make efforts to escape. If it jump away after a stimulus has been applied, it will avoid any object in its path. It will never move without some stimulus being applied. All spontaneous action has departed. 276 Nervous System It will not feed itself, but will sit still till it decom- poses (fig. 105). Fish exhibit similar phenomena : they swim about in the water ; the movements are not voluntary, but result from the stimulus of the water in contact with the body. Pigeon with cerebral hemispheres removed sits on its perch and balances itself perfectly. When thrown in the air it flies, when pinched it moves forward. If not meddled with it appears to be in a profound sleep, though occasionally it will dress its feathers or yawn. Its pupils contract normally. It resists any efforts made to open its beak, but swallows when food is placed in its mouth. It makes no spontaneous movements ; the yawning and dressing itself are pro- bably the result of the irritation of the wound. Rabbit. — When the cerebral hemispheres are re- moved the animal is at first prostrate ; after a while it can use its legs, though the fore ones are weak. If pinched it springs forward ; but, unlike frogs in a similar condition, will strike itself blindly against any obstacles in its path. When pinched severely it utters cries. In higher animals, as cats and dogs, motor paralysis is so marked after the removal of the hemispheres that no conclusions concerning equilibrium and co- ordinated movements can be drawn. At first sight it would appear that consciousness was necessary for the performance of complicated movements and the avoidance of objects in the path ; the cries elicited on pinching would appear to indi- cate the sensation of pain. Probably they are the result of a reflex mechanism, and are similar to walk- ing during sleep, or the cries elicited from patients when under chloroform. The medulla contains centres for reflex actions more complicated than the cord, and the corpora quadrigemina and cerebellum Functions of Cerebellum 277 contain centres for still more complex acts, as the reflex expression of emotion, the avoidance of an object when leaping, or the co-ordination of many contracting muscles. Functions of Corpora Quadrigemina In man they contain {nates or subjacent struc- tures') — (1) Centres for co-ordination of the movements of the eyeballs. (2) Centre for the contraction of the pupils. In some of the lower animals they contain — (3) Centres for co-ordination of retinal impres- sions with certain muscular movements. (4) Centre for maintenance of equilibrium. Ferrier found, on applying a weak interrupted current to the surface of the nates in the monkey, that irritation of one side caused the opposite pupil to become widely dilated, followed by dilatation of the pupil of same side. The eyeballs are directed upwards and to the opposite side, and the ears retracted. The legs become extended, the jaws re- tracted, and angles of mouth retracted. Irritation of the testes produces similar results, but in addition cries are elicited. Functions of the Cerebellum Removal of the cerebellum in a pigeon renders co-ordinated movements, such as walking, flying, turning round, imperfectly performed. There is no loss of muscular power or of sensation ; the bird struggles to get on to its legs and flaps its wings in its endeavour to fly, but its movements are awkward and ' irregular. Injury or a tumour of the middle lobe of the cerebellum in man gives rise to a staggering gait, not unlike that of a drunken man ; there is a difficulty 278 Nervous System in maintaining the equilibrium, especially when the eyes are closed, and in turning sharply round. Section of the middle lobe of the cerebellum in monkeys gives rise to difficulty in maintaining the equilibrium ; injury of the anterior extremity of the middle lobe causes the animal to tumble forwards, while when the posterior end is injured there is a tendency to fall backwards. Fig. 106. —Transverse section through the brain (Quain's Anatomy), cc, corpus callosum ; L V, lateral ventricle ; th thalamus ; str, lenticular nucleus of the corpus striatum ; c, caudate nucleus of the same ; between th and str is the internal capsule; outside sir is a thin gray band, the claustrum ; outside this is the fissure of Sylvius, and island of Reil ; sit, v, vessels on the surface : /, pineal gland ; B, basilar process. From these observations it would appear that in man the middle lobe of the cerebellum is in some way or another connected with the co-ordination of muscular movements, especially with those move- Basal Ganglia 279 merits which maintain the equilibrium of the body. Paralysis and muscular contractures often are present in cerebellar tumours, but these are probably due to the cerebellum compressing the mot.or tracts in the pons, or giving rise to distension of the lateral ven- tricles in consequence of pressing on the vena galeni. The functions of the lateral lobes of the cere- bellum are unknown ; they are connected by means of the fibres of the superior and inferior with the cortex of the hemispheres, and according to Gowers they are in some way connected with mental pro- cesses. Basal or Central Ganglia These consist of the corpus striatum and optic thalamus ; passing in close relation with them is the internal capsule (fig. 106). The corpus Striatum consists of two parts — an intra-ventricular portion, projecting into the lateral ventricle of the same side as an elongated grey body called the caudate nucleus (fig. 106 and fig. 107), and an extra-ventricular portion, the lenticular nucleus, more deeply placed, and is separated from the optic thalamus (see fig. 107) by the internal capsule. On its outer side it is in close proximity to the island of Reil, the claustrum intervening (see fig. 106 and fig. 107). It consists of three parts. It is uncertain if the corpus striatum has any connection with the cortex, but fibres coming from it enter the internal capsule. It consists of grey matter, and con- tains many nerve-cells. The optic thalamus is more or less oval-shaped ; it consists of grey matter, containing many nerve- cells ; it has a superficial layer of white fibres. On the inner side it projects into the lateral ventricle, being placed posteriorly to the caudate nucleus ; on its outer 28o Nervous System side is the internal capsule. It is connected with the cortex, optic tracts, and with the tegmentum of the cms. Fig. 107. — Transverse (vertical) section of a cerebral hemisphere, made in front of the optic thalamus (Landois and Stirling). CCa, corpus callo- sum ; N(J, caudate nucleus (the letters themselves are placed in the lateral ventricle); NL, lenticular nucleus; IC, internal capsule; CA, internal carotid ; a SL, lenticular striate artery (' artery of haemor- rhage ') ; F, A, L, T, motor centres governing movements of face, arm, leg, and trunk, the fibres of which converge to pass along the internal capsule. The internal capsule is a broad band of fibres which connects the cortex of the brain with the cms, and in which lie both the motor and sensory paths. Cerebrum 281 The anterior limb separates the caudate and lenticular nuclei (see fig. 107), the posterior limb lies between the latter and optic thalamus. It is best seen in horizontal section of the brain ; the two limbs are seen to join at an acute angle called ' the knee.' The functions of the corpus striatum and optic thalamus are not known with certainty. As they lie deeply, and any injury involving either almost certainly injures the internal capsule also, it is very difficult to ascertain their functions. The optic thalamus has been supposed to be in some way related to the sense of sight. According to the older view, the office of the basal ganglia was the co-ordination of muscular move- ments in the performance of complex acts : thus, to quote Broadbent, 'the corpus striatum translates volition into action or puts in execution the com- mands of the intellect ; that is, selects, so to speak, the motor nerve-nuclei in the medulla and cord appropriate for the performance of the desired action, and sends down the impulses which sets them in motion.' As already stated, the internal capsule contains the motor and sensory paths from and to the cortex, the motor path occupying the anterior two-thirds (fig. 107), and the sensory the posterior third of the hind limb. The Cerebrum The cerebral hemispheres form two ovoid masses of grey and white matter, with convolutions on their surface. The grey matter is mostly present on the surface, and forms a layer from \ to \ inch in depth, the amount being greatly increased by the convolu- tions. The white matter is arranged in various ways : longitudinal fibres, as the fornix ; transverse fibres, as the corpus callosum ; peduncular fibres, connecting 282 Nervous System the grey matter on the surface with the corpora striata (corona radiata), and the latter with the pons (crura). The grey matter of the cerebrum resembles the grey matter elsewhere, though the number and shape of the nerve-cells undergo considerable variation. Five or more layers have been described, but they blend imperceptibly into one another : — (1) The most external layer is composed mostly of neuroglia, and contains a few small cells with fine processes. (2) Contains a large number of small pyramidal or arrow-head cells. (3) Is of greater thickness, and contains large pyramidal or arrow-head cells, with their points to the surface of the convolutions. They are separated by bundles of nerve-fibres running towards the surface. (4) A narrow layer of irregular- shaped cells with fine processes. (5) Wider than the last, and composed of fusiform and irregular cells, mostly extending parallel to the surface. The large pyramidal cells present in the third and fourth layers are especially well-developed in the motor centres in the ascending frontal convolution. In the occipital lobes, and about the calcarine fissure, the large cells are very scanty. Functions of the Convolutions.— The grey matter on the surface is the seat of the higher pro- cesses of mind, including volition, memory, intellect, and the emotions. It also contains perceptive centres of special sense, as sight, hearing, touch, smell, taste. Special motor areas or centres have been localised by Ferrier in the parietal regions, and various perceptive centres in the temporo-sphenoidal lobes. The cere- Convolutions 283 bral cortex appears to contain a collection of centres, towards which incoming sensations converge from all parts of the body ; here they come into relation with one another, and give rise to motor impulses which pass to the corpora striata, and thence to the muscles. Ferrier considers the frontal lobes are connected with the intellectual faculties, and the posterior lobes with the appetites. Reference to fig. 108 will show the motor centres, stimulation of which by a weak Faradic current causes movement in the corresponding group Fig. 108. — Lateral view of left half of brain. Motor areas shaded ; dotted area indicates the speech centre (Landois and Stirling). of muscles, or destruction of which causes paralysis. The perceptive centres of vision and hearing are also mapped out ; those of touch, smell, and taste are situated in the convolution on the inner side of the temporo-sphenoidal lobe. Motor Areas. — It is from the motor centres situated at the cortex of the brain that fibres arise which pass through the white substance in the centre of the brain, along the internal capsule to the anterior pyramids of the medulla, and on to the pyramidal 284 Nervous System tracts of the cord. Injury to these centres causes, first, paralysis, and afterwards descending degeneration along the motor path takes place. The leg-centre (fig. 108) occupies the highest part of the ascending frontal and parietal convolutions on each side of the fissure of Rolando ; it also occupies the paracentral lobule on the inner surface. The arm-centre occupies the middle third of the above convolutions (fig. 108). The face-centre occupies the lower third of the ascending frontal and parietal convolutions ; the lips and tongue have their centre at the lowest part of the ascending frontal. The trunk-centre is apparently situated on the inner surface in the longitudinal fissure in front of the leg-centre. Sensory Centres. — The position of the centre for sensation of the limbs and trunk has not certainly been defined. According to Flechsig, the fibres of the sensory path of the internal capsule pass upwards to the central convolutions, i.e. ascending frontal and parietal convolutions and parietal lobe. If this view is correct, this region contains both motor and sensory centres. Ferrier localised the centre of tactile sensa- tion in the hippocampal region. In cases of injury to the brain followed by loss of motor power and loss of sensation, if recovery takes place sensation returns sooner or later, though the parts paralysed may never regain their power. This return of sensation is, no doubt, due to other parts of the brain taking on the functions of the part destroyed. Smell. — The olfactory centre is probably situated at the anterior extremity of the uncinate convolution. The experiments of Ferrier, as also anatomical and pathological researches, would seem to indicate this. Vision. — The visual area is seated in the occipital lobes ; but the exact spot has not certainly been Speech Centre 285 determined. According to Ferrier's experiments on monkeys, destruction of the angular gyri and occipital lobes cause total and permanent blindness. In man, disease of one occipital lobe causes hemianopia—thsX is, blindness of the lateral halves of both retinae corresponding to the side of the lesion. Auditory Centre, — The centre for hearing in man is situated in the first temporo-sphenoidal convolution. Destruction of this region has been followed by loss of hearing on the opposite side, though this was not permanent (Gowers). This convolution has been found atrophied in cases of congenital deafness. Speech Centre. — The centres connected with speech are in the posterior extremity of the inferior (3rd) frontal convolution of the left hemisphere ; the island of Reil is apparently also concerned. Damage to this centre is followed by the loss of the faculty of speech — a condition to which the term aphasia is applied. The patient may have perfect control over his lips, tongue, &c, and may be able to utter articu- late sounds, but he cannot combine the sounds so as to form words by which he can express his thoughts. He can neither express himself in writing, nor can he read. In some cases the patient can express his thoughts in writing when he cannot by speech. Rapidity of Cerebral Operations. — Mental operations occupy a longer period than simple reflex actions. By means of a suitable apparatus and a system of signalling this period can be approximately measured. A stimulus is applied, as an induction shock ; and the person experimented on gives a signal the instant he feels it, both being recorded on a moving surface. The interval is called ' the reaction period.' This period includes the time occupied by the im- pression in travelling along the nerve to the centre, its perception by the mind, and the time occupied by the motor impulse travelling to the muscles giving rise 286 Nervous System to muscular contraction. The reaction period for feeling is 1 sec, hearing \ sec, and sight -!- sec. But these figures are likely to vary in different persons. Course of the Motor Fibres. — The course of the motor path, or pyramidal tract, as it is called, from the parietal convolutions to the muscles is better known than the. course of the sensory path. The fibres forming the motor path on leaving the cortical centres pass through the white ' centrum ovale,' and converge to the internal capsule (fig. 107), where they occupy the anterior two- thirds or more of the posterior limb ; entering the crus cerebri, they pass along the crusta, occupying the middle two- fifths, extending from the surface below to the substantia nigra above (fig. 103) ; passing on to the pons, the fibres separate into bundles lying between the superficial and deep transverse layers, and being surrounded by much grey matter ; entering the medulla, the motor fibres rejoin one another, forming the anterior pyramids ; at the lower part of the medulla about three-fourths of the fibres decussate, passing to the opposite side to form the lateral or crossed pyramidal tract of the cord ; those which do not decussate pass down their own side of the cord, forming the ante?'ior or direct pyra- midal tract (fig. 109). Hemiplegia. — Damage to the motor centres at the cortex, or to any part of the motor path, is followed by paralysis of the face, arm, and leg of the opposite side. In a severe case the face is paralysed, chiefly the lower half ; the tongue when protruded points towards the paralysed side, and the arm and leg are completely powerless. For the most part, the muscles of mastication, respiration, and the trunk muscles escape ; these muscles, being associated in their action with those of the opposite side, are probably excited to act by the nervv-centres which supply their fellows. The commonest cause of hemiplegia is a rupture of the Sensory Fibres AO, INTERNAL CAPSULE lenticulo-striate artery and a consequent destruction of the internal capsule (fig. 109). In some forms of hemiplegia the 3rd, 4th, 6th, or other cranial nerves may be involved. Course of the Sensory Fibres. — Less is known con- cerning the path of the sensory than the motor fibres. The fibres which enter the cord from the sensory nerves mostly pass up the posterior and lateral columns of the same side and decussate in the medulla. A few pass at once on entering to the posterio-lateral column of the opposite side. In the medulla and pons, the sensory path is believed to lie in the formatio reticularis, from which it passes along the tegmen- tum of the cms and enters the internal capsilk, occupying the posterior third of the hind limb. From the internal capsule the path lies through the central white substance to the cortex, being distributed to that part of the cortex which lies be- neath the parietal bone (Flech- sig), or, according to Ferrier, to the hippocampal region. In the upper part of the pons the path is joined by the sensory fibres from the face (5th nerve), so that the posterior part of the internal capsule contains sensory fibres from the whole tra?a,/couK?ofraotSrpath- 288 Nervous System of the opposite side of the body. Apparently, also, the fibres from the special organs of sense, taste, hear- ing, smell, vision, pass through the internal capsule. Hemianesthesia. — Destruction of the posterior portion of the internal capsule gives rise to loss of sensation to touch, pain, and temperature of the oppo- site side of the body ; the organs of special sense are also involved. If the sensory path is destroyed below the pons, the parts supplied by the 5 th nerve escape. In many cases of hemiplegia there is also more or less loss of sensation. Functions of the Cranial Nerves First, or Olfactory. — The olfactory nerve arises from the under surface of the frontal lobe in front of the anterior perforated space by three roots, the ex- ternal, middle, and internal. It lies on the orbital surface of the frontal lobe, lodged in a sulcus, and swells out into an enlargement, the olfactory bulb. The branches or true olfactory nerves, some twenty in. number, pass through the cribriform plate, and ramify upon the upper part of the septum, and the superior and middle turbinated processes. The olfac- tory bulb or lobe consists of grey substance ; the peripheral nerves consist of nerve-fibres, which are non-medullated. The mucous membrane, over which the olfactory nerve is distributed, is softer and more pulpy than the respiratory region. It is of a yellow colour. The epithelial cells are modified so as to form terminal organs for the olfactory nerves. The columnar cells are continued downwards in a root- like process, and between the cells there are fine threads or rods, connected at their inner extremities with spindle-shaped olfactory cells, the cells being apparently connected with filaments of the olfactory Optic Nerve 289 nerves. The olfactory nerves do not decussate, and are unlike all other nerves in this respect. Optic Nerve. — Each optic tract arises from the posterior part of the optic thalamus and the corpora geniculata. Leaving this attachment, it winds forward, Fig. no.— Cells and terminal nerve-fibres of the olfactory region. 1, from the frog ; 2, from man. a, epithelial cell, extending into a root-like pro- cess ; b, olfactory cells ; c t peripheral rods ; d, their central filaments. 3, olfactory nerve fibre in dog. a, division into fine fibrillse. Irritation of the olfactory terminal organs gives rise to a sensation of smell. crosses the crura cerebri as a flattened band ; then, becoming more cylindrical, it passes forwards, joining its fellow of the opposite side to form the commissure, In front of the commissure the optic nerves, as they u 290 Nervous System are now called, receive a sheath from the dura mater and arachnoid, and pierce the back of the eyeball. Decussation takes place at the commissure, but this decussation is a peculiar one. According to Charcot, the decussation of the optic tracts takes place as represented in fig. 111, there being not only a crossing at the chiasma, but also Fig. hi.- -Scheme of decussation of optic tracts (Charcot), le and RE, le t and right eyes ; c, commissure ; LG and k g, left and right geniculate bojies ; Q, corpora quadrigemina : L H and R H, left and right cerebral c: lire of vision ; b and a, nerve-fibres from left and right sides respec- tively of left eye ; b' and a\ corresponding fibres from right eye. at the corpora quadrigemina. According to this view a lesion at c would intercept the fibres from both nerve-centres going to the inner side of each retina, and cause blindness of each inner half of the retina, a condition to which the term double temporal hemianopia is applied. A lesion in the optic tract behind the chiasma would intercept the fibres going to each lateral half of the retina of the same side — lateral Third Nerve 291 hemianopia. A lesion of the cortical centre (angular gyrus) would, according to this view, produce total blindness in the opposite eye. Gowers dissents from Charcot's views, and does not believe that a second crossing takes place in the corpora quadrigemina. In man, as shown by patho- logical observations, a lesion of the chiasma gives rise to double temporal hemianopia ; a lesion of the optic tract and between the chiasma and the occipital cortex gives rise to lateral hemianopia; a lesion of the cortex itself at the angular gyrus causes partial loss of vision of the opposite eye, the central portion round the yellow spot remaining sensitive to light. This condition is called crossed amblyopia. Stimulation of the optic nerve produces sensations of light ; thus flashes of light are seen when the elec- trodes of a galvanic battery are placed on the temples, and the current slowly interrupted. The optic nerve is also the afferent nerve in the reflex contraction of the pupil, in the closure of the eyelids, when a bright light falls on the retina. Third, or Oculo-motor. — This nerve takes its deep origin in a cluster of cells, situated in the floor of the aqueduct of Sylvius ; its nucleus is joined in- feriorly by the nucleus of the fourth nerve (fig. 118). From this nucleus the fibres pass through the crus to its inner side. The third nerve is purely motor, being distributed to all the muscles of the eyeball, except the superior oblique and external rectus ; it also sup- plies the circular fibres of the iris and the ciliary muscle. Paralysis of this nerve gives rise to (1) ptosis, or drooping of the eyelid in consequence of the un- opposed action of the orbicularis ; (2) the eyeball is turned outwards and downwards by the ext. rectus and sup. oblique ; (3) the pupil is dilated and fixed ; (4) the eye cannot be accommodated to near objects ; (5) there is also double vision. (12 292 Nervous System Fourth Nerve. — This nerve arises from a nu- cleus immediately below that of the third in the floor of the aqueduct. The fibres pass downwards and backwards, emerge at the upper part of the valve of Vieussens, and wind round the cms to the base of the brain. It is purely motor, and supplies the superior oblique. Paralysis of the fourth nerve causes squint, Fie. 112.— Nerves of the orbit from the outer side (Quain's Anatomy). The external rectus has been cut and turned down ; 1, optic nerve ; 2, the trunk of the third nerve; 3, its upper division passing to the levator palpebral and superior rectus ; 4, its long lower branch to the inferior oblique muscle ; 5, the sixth nerve ; 6, the Gasserian ganglion ; 7, the ophthalmic nerve ; 8, its nasal branch ; 9, the ophthalmic ganglion ; 10, its short or motor root ; n, long sensory root from the nasal nerve ; 12, sympathetic from the carotid ; 13, ciliary nerves ; 14, frontal branch of ophthalmic. the eye being directed upwards and inwards, and double vision. Fifth or Trigeminal Nerve. — This nerve emerges from the side of the pons Varolii, and con- sists of two roots, the smaller being the motor. The motor root arises from a nucleus (fig. 118, V,) imme- diately in advance of the 7th nucleus. The sensory root arises from two nuclei, the middle (V 2 ) and inferior (V 3 ) of the 5 th. The fibres pass through the pons, Fifth Nerve 293 and appear at the surface. The sensory root becomes connected with the Gasserian ganglion* from the fore part of which the three primary divisions proceed. They are (a) Ophthalmic, purely sensory to the eye and forehead, eyelids, conjunctiva, tip of nose ; secreto-motor branches to the lachrymal gland (fig. 113). Injury to this nerve causes ulceration and sloughing of the cornea. (b) Superior maxillary or second division (fig. 113) is purely sensory to skin of face, mucous membrane of the nose, and teeth of upper jaw. Pungent odours, as of ammonia, are perceived through this nerve, (c) Inferior or third division (fig. 113) is sensory to the tongue, mouth, teeth, and skin covering lower jaw. It confers tactile sensibility on the tongue, and through it pungent and acid tastes, as of pepper, vinegar, and mustard, are per- ceived. Motor filaments are supplied from its an- terior division to the muscles of mastication, including the buccinator, anterior belly of digastric and mylo- hyoid. There are four ganglia in connection with the fifth nerve : — 1. The ophthalmic or lachrymal ganglion (fig. 112). Motor root from inferior division, third; sensory . from nasal ; sympatetic from carotid. It gives off sensory motor and vaso-motor branches to the eye. 2. The spheno-palatine or Meckel's gan- glion (fig. 113). Motor root from the facial through the greater superficial petrosal ; sensory root from second division of the fifth ; sympathetic root from carotid. It gives off motor fibres to the levator palati and azygos uvulae ; sensory fibres to the mucous mem- brane of the nose and palate. 3. The otic ganglion (figs. 1 13 and 1 14). Motor root from inferior division of the fifth ; sensory root from the glosso-pharnygeal through lesser superficial 2 94 Nervous* System petrosal ; sympathetic root from the middle meningeal artery. It gives off motor fibres to the tensor tympani Fifth N. Facial N, Mylo-hyoid Fig. 113.— Diagram of the fifth nerve, its connections and branches, od, ophthalmic division ; c, frontal ; e, lachrymal ; d, nasal, s m, superior maxillary : 2, terminal branches, nasal, labial, and palpebral ; 2, recur- rent ; 3, orbital ; 4, dental ; 5, to Meckel's ganglion, 1 d, Inferior divi- sion : a, motor division joining anterior division, mostly motor, terminal branch to the mucous membrane of mouth : posterior division ; at, auri- culotemporal ; lingual to tongue ; inferior dental ; mylo-hyoid branch to digastric and mylo-hyoid. and tensor palati ; secretory fibres to the parotid gland. 4. Thesubmaxillaryganglion(rlg. 113). Motor Sixth Nerve 295 root from the facial through the chorda tympani ; sensory root from lingual of the fifth ; sympathetic root from facial artery. It gives off vaso-inhibitory fibres from the chorda tympani to the submaxillary gland ; secretory fibres also from the same source to the glands. Fifth N, Facial N. Meckel's G. Fig. 114. — Diagram of the facial nerve and its connections (after Young). Facial nerve ; r, geniculate ganglion ; gs.p., great superficial petrosal passing to Meckel's ganglion ; s.s.p., small superficial petrosal passing to the middle meningeal ; 2, chorda tympani, joining lingual ; 3, nerve to the stapedius ; 4, communicating branch with ganglion of the root of the vagus; 5, posterior auricular nerve ; 6, branch to stylo-hyoid and digas- tric. M M, middle meningeal artery ; I M, internal maxillary artery ; at, auriculotemporal nerve ; I, lingual ; id, inferior dental. Sixth or Abducent Nerve.— This nerve arises from a nucleus in the floor of the pons (fig. 118, VI.), close to the nuclei of the seventh and motor of the fifth ; it emerges between the medulla and pons. It supplies the external rectus. When this muscle is paralysed there is well-marked internal squint. 296 Nervous System Seventh or Facial. — The nucleus of the facial is in close relation (fig. 118, VII.) with the nucleus of the sixth. It emerges from the medulla between the restiform and olivary bodies. The auditory arises from three nuclei, one of which forms a convex promi- nence in the outer part of the lower half of the fourth ventricle. The facial is motor, and supplies the muscles of the face, lips, stylo-hyoid, digastric, soft palate (through the spheno-palatine ganglion), tensor palati and tensor tympani (through the otic ganglion), stapedius, external muscles of the ear, and supplies vaso-inhibitory and secretory fibres to the submaxillary and sublingual glands. It is the special muscle of expression : when injured the corresponding side of the face becomes a blank, the mouth is oblique, being dragged towards the sound side. The eye is wide open and cannot be closed, and tears run down the cheek ; food accumulates between the gum and cheek, and the pronunciation of labial consonants is rendered difficult, and movements of the nostril cease. Should injury to the nerve occur above the origin of the chorda tympani and petrosal nerves (fig. 114), there will be an interference with the sense of hearing, through paralysis of the stape- dius and tensor tympani ; dryness of one side of the tongue, owing to the implication of the chorda and submaxillary glands ; and relaxation of the soft palate and pointing of the uvula to the sound side, in consequence of paralysis of the azygos and soft palate. The auditory nerve passes into the internal audi- tory meatus, and divides into two branches ; one is distributed to the cochlea, the other to the vestibule. It fulfils two functions : it is the nerve of hearing, and also conveys, by means of the fibres distributed to the semicircular canals, information to the sensorium, Glosso-pharyngeal 297 Carotid Fig. 115.— Diagram of the glosso-pharyngeal and its connection and branches (after Young). Glosso-pharyngeal ; jg, jugular ganglion ; pg, petrosal ganglion: 1, tympanic branch ; 2, filaments to the carotid; 3, to Eusta- chian tube ; 4, to fenestra rotunda ; 5, to fenestra ovalis ; 6 and 7, to small and great superficial petrosal ; 8, pharyngeal branches ; o, to stylopha- ryngeal and constrictors ; 10 and it, tonsillitic and terminal. Vagus branches from ganglion of root : s, superior cervical ganglion. 298 Nervous System which assists in maintaining the equilibrium of the body. The Glosso-pharyngeal.— This nerve arises from a nucleus in close contact with that of the vagus (fig. 118, IX.), and is in part overlapped by the audi- tory nucleus. The fibres emerge from the lateral tract of the medulla below the auditory. This nerve (fig. 115) is the special nerve of taste for the back of the tongue ; it supplies the root of the tongue, soft palate, pharynx, and tympanum with common sensa- tion. It supplies motor fibres to the stylo-pharyngeus, middle constrictor, azygos uvulae, levator palati. It is probable, however, that the last two are supplied by the facial. The Vagus or Pneumogastric. — The nucleus of the vagus (fig. 118, X.) is situated immediately below the nucleus of the glosso-pharyngeal, and is continuous with it. It arises from the medulla, im- mediately below the glosso-pharyngeal. It contains both sensory and motor fibres, though part of the latter are derived from the spinal accessory. The vagus is distributed to three different sets of organs (fig. 116) — (a) the lungs and respiratory passages; (b) the heart ; (c) the pharynx, cesophagus, and stomach. (a) The superior laryngeal is the nerve of sensa- tion to the mucous membrane of the larynx, and supplies one muscle — the crico-thyroid. Paralysis of this nerve causes loss of sensation in the larynx, and interferes with the utterance of high notes from paralysis of the crico-thyroid. The inferior laryngeal is the' motor nerve to the intrinsic muscles of the larynx, except the crico-thyroid. Stimulation of the central end of the superior laryngeal causes a flaccid state of the diaphragm, and excites contractions of the expiratory muscles. The vagus supplies several different sets of fibres to the lungs, motor to the Glosso-Pharyng. Ganglion of trunk Pharyngeal branches Vagus Ganglion of root Hypoglossal r°^ RT^i. Spinal accessory Hepatic Gastric Splenic Fig. ii6.— Diagram of vagus, its branches and connections. 300 Nervous System muscular fibre of the bronchi, ordinary sensory fibres, and fibres which, when stimulated, excite the contrac- tion of the inspiratory muscles. (b) The vagus contains fibres which inhibit the action of the heart, by antagonising the activity of the intracardiac ganglia. Also sensory fibres which convey the sensations of pain, as in angina pectoris. (