A_Kr. ITS FEODUGTS FARR.INOTON and WOLL g»tatf (EoUpgf of AgrtruUur? At (HnrnpU Uniuprfiitg ©brary I.F. Roberts Collection ^;^.^^....^.^ Roger v.. Roberts. SF 253.F282"" """""'' '"'"^ Testing milk and its products: a manual f 3 1924 003 049 347 The original of tliis book is in tine Cornell University Library. There are no known copyright restrictions in the United States on the use of the text. http://www.archive.org/details/cu31924003049347 .■^1 Im Ti!^ S. M. BABCOCK, Ph. D, TESTING MILK AND ITS PRODUCTS. A MANUAL FUR DA1F?Y STUDENTS, CREAMERY ANL) CHEESE- FACTORY OPERATORS AND DAIRY FARMERS. BY E. H. FARRINGTON and F. W. WOLL Professor in Chargo o/ Dairy Sc/jooi Ass/ . Proy'. o/\ li,-ri. Choiidstry Of tlie Uqiversitij of Wisconsiq. JAUtli aHusiraticrno. FIRST EDITION. M.iDISijN', WIS. Mendota Book Company. 1897. ALL RIGHTS RESERVED. Copyright, i?,o-j By E. H. FARRINGTON and F. W. WQLL M. J. Cantwell, Printer Madison, Wis. PREFACE. The present volume is intended for the use of dairy students, creamery and clieese-factory operators, practical dairymen, and others interested in the testing or analysis of milk and its prod- ucts. The subject has been largely treated in a popular manner ; accuracy and clearness of statement, and systematic arrangement of the subject matter has, however, been constantly kept in mind. The aim has been to make the presentation intelligible to students with no further training than a common-school education, but their work will naturally be greatly lightened by the aid and guidance of an able teacher. Complete directions for making tests of milk and other dairy products are given; the difficulties which the beginner may meet with, are considered in detail, and suggestions offered for avoiding them. It is expected that a factory operator or practical dairy- man, by exercising ordinar}- common sense and care, can obtain a sufficient knowledge of the subject through a study of the various chapters of this book to make tests of milk, cream, etc., even if he has had no previous experience in this line. For the benefit of advanced dairy students who are somewhat familiar with chemistrv and chemical operations. Chapter XIV has been added giving detailed instruction for the complete chem- ical anah'sis of milk and other dairy products. The detection of preservatives and of artificial butter or filled cheese has also been treated in this connection. As the subject of milk testing is intimatelv connected with the paj'inent for the milk delivered at butter- and cheese factories, and with factory dividends, a chapter has been devoted to a discus- sion of the various systems of factory book-keeping, and tables iv 1 cstino jMilh and Its Products. greath' facilitating the work of the factory secretary or book keeper have been prepared and are included in the Appendix. Acknowledgment is due to the following parties for the use of electrotypes, viz: Vermont Farm Machine Co., Bellows Falls, Vt.; Cornish, Curtis and Greene Mfg. Co., Fort Atkinson, Wis.; Elgin Mfg. Co., Elgin, 111.; F.B. Fargo & Co., Lake Mills, Wis.; DeLaval Separator Co., N. Y. City: Henry Troemner, Philadelphia, Pa.; Springer Torsion Balance Co., N. Y. City; J. H. Monrad, Win- netka. 111.; Borden & Selleck Co., Chicago, 111.; Dairymen's Sup- ply Co., Philadelphia, Pa.; and the agricultural experiment sta- tions at New Haven, Conn., and Madison, Wis. University of Wisconsin, Madison, Wis., Oct. 1, 1S97. E. H. FARRIXGTOX. F. W. WOLL. TABLE OF CONTENTS. Introduction. p./yey. The need of a practical milk- and cream test. Introduction of milk tests. Short's test. Other milk tests. The Babcock test. Foreign methods. Gerber's method. DeLaval butj-rometcr. Fjord's centrifugal cream-test, ---_. 1-10 Chap. I. COMPI.ISITIOX OF MILK .\.\D ITS PRODUCTS. Water. Fat. Casein and albumen. Milk sugar (lactose). .\sh. Other components. Colostrum milk, 11-19 Cliaj). II. S.iMPLI.NG MILK. Sweet milk. Partially churned milk. Sour milk. Frozen milk, ------_... 20-24 C'liaj). III. The B.^hcock test. Directions for making the test: Sampling. Add- ing acid. Mixing milk and acid. Whirling bottles. Add- ing water. Measuring the fat. Discussion of the details of tlie test: 1. fTlass- ware. Test bottles. Marking test bottles. Cleaning test bottles. Pipettes. " Fool pipettes." .Acid measures. The Swedish acid bottle. Calibration of glassware. Cal- ibration \vith mercur}-. Cleaning mercury. Calibration with -water, a. Measuring the water, b. Weighing the water. 2. Centrifugal machines. Speed required for the complete separation of the fat. Ascertaining the neces- sary speed of testers. Hand testers. Power testers. 3. Sulfuric acid. Testing the strength of the acid. The Swedish acid tester. The color of the fat column an index to the strength of the acid used. Influence of tem- perature on the separation of fat. 4. Water to be used in the Babcock test. Reservoir for water. 5. Modifica- tions of the Babcock test. The Russian milk test. Bart- lett's modification, .-------. 25-63 vi Tcs/iii^' Jllilk (Hid //s Proditrts. ('Iiap. IV. CidiAM tkstim;. /vjes. Errors of measuring cream. Avoiding errors of measuring cream. Cream test bottles. Tlie bulb-necked cream bottles. The Winton cream bottle. T'se of milk test bottle. Use of .^i cc. pipette. Weighing the cream, (;4--73 ('liap. A'. B.MICOCK TliST FOR OTIIKK .MILK I'KODUCTS. Skim milk, butter milk, and whey. The double- necked test b(jttle. The doublesized skim milk bottle. Cheese. Condensed mill<, ------- 74-70 Cliiip. VI. Tim: i.ACTOMiiTiiK and its ai'I'I.ication. The (hievenne l.ictometer. Influence ol temper.'i- ture. N. \. Hoard of Health lactometer. Reading the lactometer. Time of taking lactometer readings. Cal- cidation *jf milk solids. Adulter,'Ltu>Ti of milk. L'fileula- tion oi extent of adulteration. Skimming. Watering. Watering and skimming. Other methods of adulteration Sii-;)3 ('hap. VII. Tksiinc, THi: ACinn "i' oiMii.K andck'Kam. Cause of aeiditv in miliv. MetlK>ds of testing acidity. Manns' test. Devarda's acidimeter. 'I'he alka- line tablet test. Acidity of cre.'im. l)eterminntion of acidity in sour cream. The standard solution used. S]iill- man's cylinder. Ka])id estimation of the acidity of a]j- parently sweet milk and cream. Detecting ]jreser\ aline in milk. " Alkaline tabs," ------- Dt-llO (.'liap. VIII. Testing tiii-: piikit'i' (jf mii.k. The Wisconsin curd test. The fermentation test, 111-f 15 €ha|). IX. Testing milk cj.^j the i-akm. \^ari.'itions in milk r>f single cows. Cause of ^-ari- ation in fat content. Number of tests r(.i|uired during a period of lactation in testing cows. When to test a cow. a. As to quality of milk produced, b. ,\s to i|uantity of milk ijroduced. fjurler's method. Rcciu-d of tests. Sam[iling milk of single cows. Size ol test sani]jle. Variations in herd mill<. Ranges in variatitjus of herd milk. Inlluence of he-ivy grain feeding im the i|u.ility of milk. Influence ol yi.isturc on the cpiality (jf milk. Method of improving the qu.'dity of milk, ----- 110-1,33 Tabic of Contents. vn C'liai). X. Composite samples of milk. rages. Methods of taking composite samples, a. Use of tin dipper, b. Drip sample, c. Scovell sampling tube. One-third sample pipette. .Accuracy of the described methods of sampling. Preservatives for composite sam- ples. Bi-chromate of potash. Other preservatives. Care of composite samples. Fallacy of averaging percent- ages. A patron's dilemma, -.,.._ 13-t-]4& Clia|». XT. Cre.am testlng at cream-gathekino CREAMEKIKS. The space system. l~he oil test churn. The Bab- cock test tor cream. Sampling tulje. Sampling cream for composite testing, - 150-159 Chap. XII. Calculation of butter and cheese YIELDS. Calculation oi yield oi butter: Butter fat test and yiehl of butter. Variations in composition of but- ter. Overrun of chvirn over test. Factors influencing the overrun. Calculation of overrun. Conversion factor for butter fat. Butter j'ield from milk of different richness, a. Use of butter chart, b. T^se of overrun table. Calcu- tion of yield of cheese: a. From fat. b. From solids not fat and fat. c. Froni casein and fat, - - - . 16i)-173 Chap. XIII. Calculatlng dividends. Calculating dividends at creameries: Proprie- tary creameries. Co-operative creameries. Illustrations of calculations of dividends. Other systems of payments. Paving for butter delivered. Relative value tables. Cal- culating dividends at cheese factories: Proprietary fac- tories. Co-operative faciories. Illvistrations of calcula- tions of dividends, .----.-- 174-185 Chap. XIV. Chemical analysis of milk and its PRODUCTS. A. Milk: Specific gravity. Water. .Alternate method. Fat. Casein and albumen. Van Slyke's method. Alilk sugar. Ash. Acidity of milk. Detection of preser- vatives in milk. Boracic acid. Bi-carbonate of soda. Fluorids. Salicylic acid. Formalin. B. Skim milk, but- viii Testing- jl/ilk and lis Products. ter milk ami whey- C. Butter. Sampling. Determina- Pages. tion of water. Fat. Casein. Ash. Complete analj'sis of butter in the same sample. Detection of artificial but- ter. Filtering the butter fat. Specific gravit3'. Reichert- Wollnj' method (Volatile acids). D. Cheese. Water. Fat. Casein. Ash. Other constituents. Detection of oleomargarine cheese {" Filled " cheese ) - - - - 186-20.3 Aiipeudix. Table I. Composition of milk and its products. Table II. Milk standards. Table III. Ouevenne lactometer degrees corres- ponding to the scale of N. Y. Board of Health lactome- ters. Table IV. Correction table for specific gravit_v of milk. Table V. Percent of solids not fat, correspond- ing to to G percent of fat and lactometer readings of 26 to 36. Directions for the use of tables YI, YII and IX. Table YI. Pounds of fat in 1 to 10,000 pounds of milk testing 3 to 5.35 percent. Table YII. Amount due for butter fat, in dol- lars and cents, at 12 to 25 cents per pound. Table YIII. Relative value tables. Table IX. Butter chart, showing calculated yield of butter, in pounds, from 1 to 10,000 pounds of milk testing 3.0 to 5.3 percent of fat. Table X. Overrun table, showing pounds of but- ter from 100 pounds of milk. Table XI. Yield of cheese, corresponding to 2.5 to 6 percent of fat, with lactometer readings of 26 to 36. Table XII. Comparisons of Fahrenheit and Centigrade (Celcius) thermometer scales. Table XIII. Comparison of metric and custom- ary weights and measures, Suggestions regarding the organization of co- operative creameries and cheese factories, - - - 205-232 Index, - - - - - 233-236 TESTING MITK AND TFS PRODUCTS. INTRODUCTION. The need of a rapid, accurate and inex[iensiYe method (.>f determining the amount of butler fat in milt; and other dairy products hecame more and more apparent, in this country and al.n'oad, with tlie progress of the dairy industry, and especially' with the growth of the factory system of but- ter and cheese making during the last few decades. So long as each farmer made his own butter and sold it to |:iri- vate customers or at the village gr<:ieery, it was not a matter of much importance to others wliether the milk produced bv his cows was rich or poor. P)Ut as creameries and cheese factories multiplied, and farmers in the dairy sections of our country became to a large extent patrons of one or the other of these, a system of equitalile payment for the milk or cream delivered became a vital question. 1. The need of a practical milk= and cream test. The creameries in existence in this couutrj' up to within ten years were nearly all conducted on the cream-gatheiing plan: the different patrons set their milk, and cream gatherers hauled the cream to the creamery, usualh' twice or three times a week, where the mixed lots of cream were then ripened and churned. The patrons were paid per inch of cream furnished; a creamery inch is a quantity of cream which fills a can twelve inches in diameter, one inch high, or 113 cubic inches. This ijuantity of cream is sup- 2 Testing Milk and Its Products. posed to in:ikc a pound of Ijutter, but cream from different sources, or even from the same sources at different times, varies greatly in butter-producing capacit}^, as will be shown under the subject of cream testing (140"). The sj'stem of paj'ing for the number of creamery inches delivered could not therefore long give satisfaction. The proposition to take out a small portion, a pint or half a pint of the cream furnished by each patron, and deter- mine the amount of butter which these samples would make on being churned in so-called test churns, found l:)ut a ver}' limited acceptance, on account of the labor involved and the difficult}' of producing a first-class article of all the small batches of butter thus obtained. 2. The introduction of the so-called o/? test claxrn (1S7) in creameries, which followed the creamery inch system, marked a decided step in advance, and it soon came into general use in cream-gathering districts. In this test, glass tubes of al)Out ^ inch internal diameter and nine inches long, are filled with cream to a depth of 5 inches, and the cream churned; the tubes are then placed in hot water, and the column of melted butter formed at the top is read off by means of a scale showing the number of pounds of but- ter per creamery inch corresp(jnding to different depths of melted butter. A\'hile the oil test is capalile of showing the difference between good and poor cream, it can not, accord- ing to investigations conducted at the Wisconsin experiment station, make strictly accurate distinctions between dilfercnt grades of good anariU i vi' iiualyHr.s madt.' Iiy the Balirock tust and gravimetric allaly^i^; up to ]>yL', .^l-o Ilnai'dV I>airyuiaii, Cict. 7. IS'.tL', p. 2.^i;0; also Bclirott, jnirlizriluiiH, l.s'.iii, |i. 183, cl serj. Introdiictivii. 7 or other dairy products may be left for months, if desired, before the test is completed, and correct results still fie obtained. The completed tests will keep indefinitely in the fiottles. so that the results may fie verified at any future time, if desired. Sour milk uiaj' be anal3zed with perfect :issurance of accurate results, provided it can be proi)er!y sampled. The test is finally applicalile, besides to full milk, to cream, skim milk, butter milk, whe}'. condensed milk and (if a small scale for weighing out the sample is available) to cheese. 10. With all these advantages, the Babcock milk test is not in every respect an ideal test. The handling of the very corrosive sulfuric acid requires constant care and attention; the speed of the tester, the strength of the acid, the temperature ot the milk to be tested, and other points, alwaj's require watching, lest the results olituined lie too low, or otherwise unsatisfactory. The test is. however, for general purposes, in the opinion nf the writers, the verv best at our disposal, and in the hands of careful intelligent operators, will easil}- give most satisfactory results. 11. Foreign methods. In I'luropeau countries three practical milk and cream tests, besides the liabeock test, are in use at the present time, \\7. ; Gerljers aciJ-hnli/nime- ter, De Laval's hutjjranieler. :\ni\ FjariTs cent rifuijal cream ttst.'' Of these, the last test gi\'en has ne\'er been introduced into this country, to our knowledge, and the former two. only on a small scale. * The Lister-Balicock milk test advertised in EngHsli papers and known as such ill England, is the regular Babcock test, to which llie linglish manufacturers have preli.Ked tlieir name. s Testing- Milk and /Is Producls. 12. The Qerber method* (fig. ]) is essentially the old iJeimling method worked out independently by the Swiss chemist, Dr. N. Gerber. In this test sulfuric acid of the same strength is used as in the liabcock test, and a small quantity of amyl alcohol is added. The amyl alcohol facili- tates the separation of the fat, but introduces a source of error which may Iteeome serious, and especially so, where the results obtained with a Fig. 1. Tli.Mlerbcra,.i.J-l,utyrornetrr. ng^v lot yf amyl alcohol can not be compared with gravimetric anal3'sis or with tests made with amyl alcohol known to give correct results. ll{. In the De Laval butyrometer (flg. 1') the same acid is used as in the Babcock test, but the tubes employed, and the manipulations of the method differ materially from this test; a smaller sample of milk is taken (only 2 cc.) and a correspondingly' small <|uantity of acid used. The results oljtained are correct. Where a large number of milk samples are tested every day, as is the case, for instance, in European milk control stations, the but\romcter may be preferable to the iJabcock test; liut it requires more skill of the operator and is more dilHcult to work satisfactorily in case of milk whirh cannot be easily sampled, as sour, lop- pered, or [jartially churned milk. 'J'he machines placed on * (;erl)c:r, l>Mt Cruklisc hr Milch-l'riicCiniL'. Introducliou. 9 the market both by Dr. Gerber ami the De Laval Company are more expensive than the Bal3cock testers sold in this country; the De Laval test requires a high speed. 5-0000 Fl '^ DeLa\al3 1 tyr meter revolutions per minute, and therefore places greater de- mands for soliditjMn the machine than does the Babeoek test. 1-t. Fjord's centrifugal cream tester* (fig. 3) is ex- tensively used in Denmark and is mentioned in this connec- tion as it furnishes a fairly reliable method for compar- ing the quality of different lots of milk. The method was pul)lished in 1S7S, hj the late X. J. Fjord, director of the state experiment sta- tion in Copenhagen, through whose exertions and on state Danisli experiment station, < 'openLiagen, sixtli ananish herd milk, the results obtained correspond to the percents of fat present in the samples, one per cent, of cream being equal to aliout 0.7 per cent, of fat; outside of these limits the test is, however, unrelialile, especiallj' in case of very rich milk and strippers' milk. Only sweet milk can be tested by this method. The recent introduction of milk tests proper into Jieiunark, like the Gerber, Babcock and J)e Laval tests, will, however, in all probabilit}' in time force the Fjord cream test out of ]>anish creameries, for similar reasons that relegated to obscurity the gravit}- cream tests (creamometers.) Hefore going over to the main part of the present work, the discussion of the Babcock milk test, a brief description of the chemistry of milk and its products is given, so that the student may understand what are the components of dairy produi;ts, and the relation of these to each other. Only such points as have a direct bearing on the subject of milk testing and the use of milk tests in butler and cheese fac- tories or pri\'ate dairies will be treated in this cha|)tcr. and the reader is referred to standard works on dairying for further information in regard to the composition of dairy products. Composition of Alilk and Its Products. ii Cli AFTER I, COMPOSITION OF MILK AND ITS PRODUCTS. 15. Jlilk is comi)osed of the following substances: water, fat, casein, albumen, millv sugar and ash. A few other sul)stances are present in small quantities, but are of no practical importance and will not l)e considered here. The components of the milk less the water are known, collect- ively, as mill- solids, or total solids, and the total solids less the fat, i. e. casein, albumen, milk sugar, and ash, are often spoken of as solids not fat, or the non-fatty milk solids. The mil/c serum includes all components of the milk less the fat; the serum solids are therefore another name for the solids not fat; when given, they are, however, generalh' calculated to per cent, of milk serum, not of milk. If e. g., a sample of milk contains per cent, of solids not fat, and .■■; per cent, of fat, the milk serum will make up '.'T per cent, of the milk, and the serum solids, ..IV = '.I.2S pjer cent, of the milk serum. 1(). Water. The amount of water contained in milk ranges from si) to 90 per cent. Normal cows' milk will not as a rule contain more than sS per cent, of water, nor less than S4 per cent. In states where there are laws regulating the sale of milk, as is the case m eighteen states in the I'nion (see Ajq-iendi.c), the maximum limit for water in milk in all instances tint one (South Carolina) is SS per cent.: the state mentioned allows S.s.5 per cent, of water in milk offered 12 Testing Milk end Its Products. for sale within her borders. The effect of fraudulently in- creasing the water content of milk by watering is considered under Adulteration of Milk. 17. Fat. The fat in milk is not in solution, but sus- pended as very minute globules, which form iin emulsion with the milk serum; the globules are present in immense numbers, viz: on the average about one hundred million in a single drop of milk; a quart of milk will contain aljout two thousand billions of fat globules, a number written with thirteen ciphers. The size of the globules in the milk from the same cows varies according to the stage of the period of lactation, the globules being largest at the beginning of the lactation period, and gradually decreasing in size with its progress. Different breeds of cows have fat globules of different average sizes; the Channel Island cows are thus noted for the relatively large fat globules of their milk, while the Lowland breeds, the Ayrshire, and other breeds have uniformly smaller globules. The diameter of average sized fat globules in fresh milkers is about 0.0<14 millimeter, or one-six thousandth of an inch; that is, it takes about six tliousand sucli globules placed side by side to cover one inch in length. The globules in any sample of milk vary greatly in size; tlie largest globules are recovered in the cream when the milk is set, or run through a cream sepa- rator, and the smallest ones remain in the skim milk; prop- erly skimmed separator skim milk will contain (jnly a small number of very minute fat globuli'S. Milk fat is comiiosed of so-called glycerides of the fatty acids, i. e. compounds of the latter with gl^'cerine; some of the fatty acids are insoluble in water, viz: palmitic, stearic, and oleic acids, while otiiers are soluble and volatile, the Cojiiposition of JMilk and Its Products. 13 cbief ones among the latter being butyric, capr\'lic, and caproic acids. Tlie glycerides of tLie insoIiil)le fatty acids make up about 92 per cent, of tlie pure millc fat. and about S per cent, of the glycerides of volatile fatty acids are there- fore found in natural milk- (and butter-) fat. Tlie distinc- tion between natural and artificial butter lies mainly in this point, since artificial l)utter (butterine, oleomargarine) as well as other solid animal fats contain only a very small (juantity of volatile fatty acids. The glycerides of the vola- tile fatty acids are unstable compounds, easily decomposed through the action of liacteria or light; the "\(jlatile fatty acids thus set free, mainly Ijutvric acid, are the cause of the unpleasant odor met with in rancid butter. The per cent, of fat in cows' milk is generally between three and six per cent, American milk contains on the average toward four per cent, of fat. The milk from single cows in perfect health ^yill occasionally go below or above the limits gi\-eu, but the mixed milk from a whole herd rarely falls outside of these limits. The legal standard for fat in milk in most states of the Union is :! per cent. ; l\bode Island allows milk containing 2.5 per cent, of, fat to be sold as pure, while Georgia and Minnesota require it to contain 3.5 per cent., and jNIassachusetts 3.7 per cent, (in the mouths of Maj' and June; see Appendix.) IS. Casein and albumen. These belong to the so- called nitrogenous substances, distinguished from the other components of the milk by the fact that they contain the element nitrogen. Another name is albuminoids or protein compounds. Casein is precipitated by rennet in the presence of soluble calcium salts, and by dilute acids and certain chemicals; albumen is not acted upon by these I |, Trs/i/ii;- j\lilk and lis Products. agents, but is coagulated \)^ heat, a temperature of 170 F. being suflieient to effect a perfect coagulation. The casein, with fat and water, form the main components of most kinds of cheese; in the manufacture of cheddar and most other solid cheeses, casein is coagulated by rennet, and the curd thus formed holds fat anil whey mechanically, the latter containing in solution small quantities of non-fatty milk solids. The albumen goes into the whey, and in some countries is also made into cheese by evaporating the whey under constant stirring; usually whole milk of cows or goats is added and incorporated into such cheese i prbnost, goat cheese). Casein is present in milk partly in solution, in the same way as milk sugar, soluble ash-materials and albumen, and partly in suspension, in an extremely fine colloidal condi- tion, mixed or combined with insoluble calcium phosphates. The casein and calcium phosphates in suspension in milk may be retained on a filter made of porous cla}' (so-called Chamlerluiid fillers). About S(l [jer cent, of the nitrogenous coini.iounds nf nor- mal cows' milk are made up of casein; the rest is largely albumen. If the amount of casein in milk be determined by precipitation with rennet or dilute acids, and the albu- men bj' boiling the filtrate from the casein precipitate, it will be found that the sum of these two compounds does not make up the total quantity of nitrogenous constituents in the milk. The small remaining portion (about five per cent, of the total nitrogenous constituents) is called by vari- ous authors, globulin, albumosc, hemi-albumosc, nuclein, proteose, etc. The nitrogenous constituents of milk are very unstable com[)ounds, and their stud}' presents manj' and great dilliculties; as a result we find that no two seien- Composition of j\[ilk iiinl /ts Produrls. 15 tists who have made a special study of these compoimds agree as to their properties, aside from those of casein and allniuien, or their relation to the nitrogenous substances found elsewhere in the animal body. For our purpose we maj', however, consider the nitrogen compounds of milk as made up of casein and albumen, and the term casein and alhumen used in this book is meant to include the total nitrogenous constituents of milk, as obtained liv multiplying the total nitrogen content of the milk by (1.25. ■ The quantitj' of casein in normal cows milk will vary from 2 to 3.5 per cent., and of albumen from .5 to .^^ per cent. The total content of casein and albumen will range between 2.5 and 4.2 per cent., the average being about 3.5 per cent. ]Milk with a low fat content will contain more casein and albumen, than fat, while the reverse is generally true in case of milk containing more than 3.5 per cent, of fat. HI. Milk sugar or lactose belongs to the group of or- ganic compounds known as carln/JiyJrates. It is a commer- cial product manufactured from wliey, and is ol)tained in this process as pale white crystals, of less sweet taste, and less soluble in water than ordinary sugar (cane sugar, sucrose). About TO per cent, of the solids in the whey, and .33 per cent, of the milk solids, are composed of milk sugar. When milk is left standing for some time, viz: from one to several days, according to the temperature of the surround- ing medium, it will turn sour, and soon liecome thick and loppered. This change in the composition and the appear- ance of the milk is brought about through the action of * The lactor ij:ld is generally used for oljtaiiiitig the caseiD aud alhumeu from the total Ditrogen in the millc, although 6.37 would be more correct, siDce these suhstauces, accordiug to our best authorities, coutaiu ou the average 1.5,7 per ceut. of uitrogeu. 1 6 Tcslin^' Milk mid lis Prodiicls. acid-forming bacteria on tlie milk sugar; these are present in ordinarj' milk in immense numbers, and under favoraljle conditions of temperature multiply rapidly, feeding on the milk sugar as they grow, and decomposing it into lactic acid. When this change alone occurs, there is not neces- sarily a loss in the nutritive value of the milk, since the milk sugar breaks up directly into lactic acid; this is shown by the following chemical formala: C,„H.,„0,,. II,_,0 (lfictose) = \ C;;H,0;, {laclic aciiLf (Ordinarily the souring of milk is, however, more com- plicated, and other organic bodies, like butyric acid, alcohol, etc., and gases like carbonic acid and hydrogen arc formed, resulting in a loss in the feeding value of the milk, While sour milk may therefore contain a somewhat smaller proportion of food elements than sweet milk, the feeding of it to farm animals, especially' pigs, will generally produce better results than is obtained in feeding similar milk in a sweet condition. The cause of this ma}' lie in the stimulaling elfect of the lactic acid of the sour milk on the appetites of the animals, or in its aiding digestion by in- creasing the acidity of the stomach juices. That the souring of milk is due to the activities of bac- teria present therein is shown clearh- l.iy the fact that steril milk, i. e., milk in which all germ life has been killed, will remain sweet for any length ot time when kept free from infection. The amount of milk sugar found in normal cows' milk varies from 3 to fl per cent., the average content being about ■*■ One molecule of milk sugar is LOiupo^ed of 12 atoms of (.arbon (C)^ 22 atoms of hydrogen (H), 11 atoms of oxyRCn (O), and one molecule of water (H2O). In the name way, the lactic acid molecule consists of ;i atoms of carbon, 1; atoms of hydrogen, and ;t atoms of oxygen. Composition of iMili: and lis Products. 17 5 per cent.; in sour milk this content will be decreased to toward 4 per cent. '20. Ash. The ash or mineral substances of milk are largely composed of chlorids and phosphates of sodium, potassium, magnesium and calcium; iron oxid, and sulfuric and citric acids are also present in small quantities among the normal mineral milk constituents. The amounts of the different bases and acids found in milk ash have been deter- mined by a number of chemists; the average figures ob- tained are given in the following table, calculated per lOU parts of milk (containing .75 per cent, of ash) and per lOo parts of milk ash. Mineral Components c)f Mill;. In per CPiil. of Milk. In jicr cenf. of .4.^h. Potassium oxid ( K.,0 1 19 per ct. 25. 111. per ct. Sodium oxid iNaolTl 00 12.4-5 LimelCaOl is 24.38 Magnesia ( .MgO 1 02 3.09 Iron oxid (Fe,0.,) 002 .34- Phosphoric anliydrid ( I'.jO-, ) 16 21.24 Chlorin (Cll 12 16.31 .7(.".2 per ct. I(i3.6s per ct. Less oxygen, corresponding to chlorin 012 3. 68 .To per ct. lOO.OO per ct. The combinations in which the preceding bases and acids are contained in the milk are not known with certainty; the following scheme is, however, given on the best authority and is believed to be substantially correct. 2 l8 Tcslint; Milk and Its Prodiirls. Percfiifagf Composition of Milk Anh, (iSi-eldner). Sodium chlurid (common salt ) 10.02 per ct. Potassium chlorifl 'J. 16 Mono-potassium jihosphate 12.77 Di-|JOtassium jjliosphate 9.22 Potassium citr. ate 5.47 Di-maKnesium phosphate .3.71 Magnesium citrate 4-. 05 Di-caleium phosphate 7.-12 Tri-calcium phosphate K.UO Calcium citrate... 23.55 Lime comljined with casein 5.13 100.00 According to the same author, 36 to 50 per cent, of the pho.sphoric acid found in milk, and from 58 to 7i' per cent, of the lime, are present in suspension in the milk, as di and tri-calcium phosphates, and may be filtered out by means of ( 'hamberland filters (18), or Ijy long continued eentrifuging (Babcock*.) The rest of the ash constituents are dissolved in the milk serum. The ash content of normal cows' milk varies but little, as a rule only between .(5 and .8 per cent., with an average of .7 per cent. !Milk with a high fat content generally con- tains about .8 per cent, of ash; strippers' milk always has a high ash content, at times even exeeeiling one per cent. Ordinarily, the mineral constituents of milk are, however, the components least lialile to variations. 21. Other components. iSesides the milk constituents enumerated and described in the preceding pages, normal milk contains a numl)er of substances which are only pres- ent in small (juantities and have only scientific interest, such * Wi^cocsin experiiiienl Htatiur), twulftli rej»ort, p. 93. Composiiio)i of Milk and Its Products. rg as the milk gases (carhoiiio acid, oxyg'oii, nitrogen), citric acid, lecithin, cholesterin, urea, hj'poxanthin, lactochrome, etc. The percentage composition of cows' millv will be seen from the tallies given in the Appendix. Tallies are also given showing the average composition of milk products, like skim milk, butter milk, whey, cream, butter, cheese and condensed milk. 22. Colostrum milk. The liquid secreted directly after parturition is known as colostrum milk or Ijiestings. It is a thick, yellowish, viscous licjuid; its high content of albumen and ash is characteristic, and also its low content of milk sugar. Owing to the large quantity of alliumen which colostrum contains it will coagulate on being heated to boiling. The secretion of the udder gradually changes from colostrum to normal milk in the course of tour to fne days; the milk is considered lit for direct consumption, or for the manufacture of cheese and butter when it docs not coagu- lated on boiling, and is of normal appearance as regards color, taste, and other properties. For composition of colos- trum milk, see Appendix. 20 Tcs/ino- Milk and /Is Products. CriAPTKR 11 SAMPLING MILK. 2;{. The hutler lat in millc is not in solution, lilte sugar dissolved in water, but tlie minute fat globules or drops, in which form it occurs, are held in suspension in the milk scrum (17). Being lighter than the serum, the fat globules have a tendenc}' to rise to the surface of the niill^. If, there- fore, a sample of milk is left undisturbed for e\-en a short time, the upper layer will contain more fat than the lower portion. Tliis fact should always l)e liorne in mind when millc is sampled. The rapidity with which fat rises in milk can be easily demonstrated by leaving a cpiantity of sweet milk undistui'lied in a cylinder or milk can for a few minutes, and testing separately the top, middle and bottom layer of this milk. Exi'I':kimI':xt. Fill the cylinder used for making the lactoiiieter test ( 1 no I with milk, tlKjrouf^dilv tni.xed l)y pouriiyt;; me.isure a |)i|)- ctteful of milk immediately into test l)(>tt]c .'\. Alliuv the milk in the eyiinder to remain ii ndistiirljcil fur ten minntes, and then measure a jjipctteful of milk from the top of tli.at in the e^dinder, into test bottle H. Ne.xt pour out most of the milk from the cylinder, and measirre into test bottle C, :\ pipcttefnl of the last ])ortion of the milk in the c\dinder. Alter eompleliirn the tests ol .\, Ii, and C, in the iisu.al manner (.'!!!), record the results of e.aeh test in the note book. 24. 'i'lie amountof mixing necessary to evenly distribute the constituents of milk throughout its mass, can also be Sampling Milk. 21 demoustvated by adding a few drops of cheese color to a quart of milk. The yellow streaks through the milk will be noticed until it has been poured several times from one vessel to another, when the milk will have a uniform pale yellow color. Stirring with a stick or a dipper will not produce an even mixture so quicklj' or so completelv as pouring the milk a few times from one vessel to another, and in sampling milk for testing it should always be mixed l>v pouring just before the milk is measured into the Ijottle; if se\'eral tests are made of a sample, the milk should be poured ).)efore each sampling. ^l7^. Partially churned milk. A second difficulty some- times met with in sampling whole milk arises from the fact that a part of the Initter fat may be separated in the form of small flatter granules by too zealous mixing, or by reckless shaking in preparing the sample for testing. This will happen most readilv in case of milk from fresh cows, or with milk containing exceptionally large fat gloliules. ^Vhen some of the ))utter granules are thus churned out, they very quickly rise to the surface of the milk after pouring, and cannot again fie incorporated in the milk hx simple mixing; it is, therefore, impossible to olitain a fair sample of such milk for testing, without taking special measures which will be explained in the following. The granules of butter may be so small as to pass into the pipette with the milk, and the quantity measured thus contain a fair proportion of them, but they will be found sticking to the inside of the pipette when this is emptied, and so fail to be carried into the test bottle with the milk, A similar partial churning of the milk will sometimes take pla(.'e in the transportation cans. AVhen such milk is received 2 2 TcslDiii A/ill- (Hid Its Products.. at the rui'toi'V, the Imtter granules are eaiiglit ))y the strainer cloth through which the milk is poured, and thus lost both to the factory and to the farmer. This separated fat cannot ))e put into tlie cream, or added to the granular butter, with- out running the risk (if making mottled l)utter, and it will not enter into the sample of milk taken for testing purposes. When milk samples are sent in small bottles liy mail or express, or carried to the [ilacc of testing, they verj' often arrive with lumps of l)utter floating in the milk or sticking to the glass. This churning of the milk can lie easily pre- vented by filling the liottle or the can comiiletely with milk. If there is no space left for the milk in which to splash around, tlie fat will not be churned out in transit. 2(). Approximatelj' accurate results ma}' generallj- be obtained with a partiallj' churned sample of milk, if a tea- spoonful of ether is added to it. After adding the ether, cork the bottle and shake it until the lumps of butter are dissolved in the ether. This ether solution of the butter will mix with the milk, and from the mixture a uniform sample may generally be taken without difliculty. The dilution of milk by the ether introduces an crrfir in the test- ing, and only the smallest (juantity of ether necessarj' to dissolve the lumps of butter should ))e used. If desired, a definite (juantitj' of ether, say 5 or 10 per cent, of the volume of the sample of milk to be tested, may Im^ added; in such cases the result of the test must lie increased liy the percent, of ether added. liXAMi'i.K. Tci a 4 oz. sample (11!0 cc.l of ]iartl.'illy cliurned milk, fi per cent., or Ci cc, i>rcommon ether- arc added: the mixture gives an average test of 4-, 2 per cent. The test must be increased l-iy I [;,,X-'---^--l, and the original milk, therefnre contained 4-. 2 -{-.21^4.41 per cent, of fat. Siinipliiii; Milk. 23 Instead of adding ether to pavliallv cLiarued ^auiiiles. it has been suggested to warm tlie milk to lln" F. for a sutfi- cieutl)' long time to melt the butter granules; the sample is now shaken vigorousl}' until a uniform mixture of milk and melted butter is olitainetl, and a pipetteful then drawn from the sample. 'I "i . Sampling sour milk. When milk fiecomes sour, the casein is coagulated and the mechanical condition of the milk therebj' changed so as to render a proper sampling verj' diffi- cult. The l3utter fat is not. however, changed in the process of souring: this has Ijeen shown b]- one of us in a series of tests which were measured from one sample of sweet milk into six test bottles. A test ol' the milk in one of these test bottles was made everj- month for six months, and ap- proximatelj' the same amount of fat was olitained in the tests throughout the series, as was found originall_y in the milk when tested in a sweet condition.* If the milk is in condition to be sampled, the souring of it does not therefore interfere with its being tested by the Babcock test, or with the accurac)- of the results olitained. In order to facilitate the sampling of sour or loppered milk, some chemical is added which will re-dissolve the coagulated casein and produce a uniform mixture, that can be readil}- measured with a pipette. Any alkali (powdered potash or soda, or licpiid ammonia) will produce this effect. Only a very small quantit}- of powdered alkali is necessary for this purpose. The complete action of the alkali on sour milk requires a little time, and the operator should not try to hasten the solution Viy adding too much alkali. An ex- *See Hoard's Dairyman, April 3, 18'J2, Tlie same liolds true for cream, a3 shown by Winton, (U. S. Dept. Agr., Div. of Cliemistry, bull. 43, p. \V1.) 24 Testing Milk and Its Products. cess of alkali will often cause such a violent action of the sulfuric acid on the milk to which the acid is added, (on ac- count of the heat generated or the presence of carljonates in the alkali) that the mixture will spurt out of the neck of the test bottle, when it is shaken in mixing the milk and the acid. When powdered alkali is added to the milk, it should be allowed to stand for a while, with frequent stirring, until the curd is all dissoh'cd, and an even translucent liquid is obtained. Such milk may Ijecome dark colored l)y the action of the alkali, but this color does not interfere with the accuracy of the test. Instead of powdered soda or potash, these substances dis- solved in water (soda or potash 13'e), or strong ammonia water, may be used for the purpose of dissolving the coagu- lated casein in a sample of sour milk. lu this case, a defi- nite proportion of alkali solution must, however, fie taken, 5 per cent, of the volume of milk being usuallj' sufficient, and the results obtained are increased accordiugl}'. (See example cited on p. 22). '.JS. Sampling frozen milk. When milk freezes, it separates into two distinct portions: 3Iilk cr3'stals, largelj' made up of water, with a small admixture of fat and other solids, and a liquid portion, containing nearly all the solids of the milk. In sampling frozen milk it is there- fore essential that hutli the liquid and the frozen part be warmed and mixed thoroughly on tlie disappearance of the crystals, by pouring gently back and forth from one vessel into another; the sample is then taken and the test proceeded with ill the ordinary manner (i!2). Tlie Bahcock Test. 25 CnAl'TER III. THE BABCOCK TEST. '29. The Babeoek test is founded on the fact that strong sulfuric acid will dissolve all non-fatt}- solid constituents of milk and other ilairy products, and will set free the fat. This will separate on standing, but to ett'ect a speedy and complete separa- tion, the bottles holding the mixture of milk and acid are placed in a centrifugal machine — a so-called tester, and whirled for five min- utes; hot water is then added so as tn bring the li(iuid fat into the graduated neck of the test bottles, and after a repeated whirling, the length of the col- umn of fat is read off, Fl(i, 4. The Ij 1st Babcork tester miidc. showing the per Cent. of fat contained in the sample tested. 20 Tcsl/iii^ I\filk and Its Products. Sulfuric acid is preferable to other strong mineral acids for the purpose mentioned, on account of its affinity for water; when mixed with milk, the mixture heats greatly, thus keeping the fat liquid without the a[)plication of arti- ficial heat, and rendering possible a distinct reading of the column of fat brought into the neck of the test Ijottles. 30. So far as is known, any kind of milk can be tested by the Babcock test. Breed, period of lactation, qualitj' or age of the milk is of no importance in using this method, so long as a fair sample of the milk can be secured. Sam- ples of milk or other dairy products, rich in solids, require a little more effort to perfect a thorough mixture witli the acnd than thin milk or other dairy products low in solids, like whey, which ma}' be readily mixed with the acid. A — Directions for Making the Test. 31. The various steps in the manipulation of the Bab- cock test are discussed in the following pages; attention is drawn to the dilHculties which the beginner and others maj' meet with in working tlie test, and the necessary' precautions to be observed in order to obtain accurate and satisfactory results are explained in detail. The effort has been to treat the sul)iect cxhaustivel}', and from a practical point of view, so that persons as 3'ot unfamiliar with the test may turn to the pages of this book for help in any dilHculties which they ma}- encounter in their work in this line. '•VI. Sampling. The sample to be tested is first mixed by pouring the milk from one \'cssel to another two or three times so that every i)ortion thereof will contain a uniform amount of butter fat. The measuring ))ipette which has a capacity of ]7.() cubic centimeters, (see fig. (1), is filled with the milk immediatel}- after the mixing is completed, by The Bahrock Test. sucking the milk into it until this rises a little abo\'c the mark around tiic stem ot the pipette the forefinger is then (juickl^y placed o\er the end of the pipette before the milk runs down Ijclow the mark. By loosening a little the pressure of the finger on the end of the pipette, the milk is now al- ■■SjiSjiP lowed to run down until it just reaches the mark on the stem the (pumtitj' of milk contained in the pipette will then, if this is cor- !jij|| recth' made, be exactl}' 17.6 cc The finger should l.ie dry in meas uring out the milk so that the de- livery of milk may be ('hecked I13 gentle pressure on the upper end of the pipette. The point of the pipette is now placed in the neck of a F>alicock test bottle (fig. 5) and the milk is allowed to flow slowly down the inside of the neck. Care must lie taken that none - Milk and Its Prodtic/s. the bottle in tliis way, there is .1 (hmgor that some of the milk may coiiii)lotely lill the neck of the bottle, and as a result, (low over the top of the neck. I'liJ. 7. Till' rinlit Wiiy itf jilyiii^ piprllc iiilo li'sl Ih.UI,.. ;{;{. Addinjr acid. Tlii' Mcid cylinilci-, (li^f. II), hnlcliiiL,' 17. .'ice.,, is then lilird to the marlc with siiifiii'it^ acid of a spccilii- jfi'avil.y of I.Hl' l.s;;. 'riiis aiiKuiht (if at'id is care- fully poured into llic test bottle containing the milk. In addin}^ the acid, the test bcjLtle is conveniently held at an The, Babcock Test. 29 angle, (see fig. 7), so that the acid will follow the wall of the bottle and not run in a small stream into the center of the milk. ])}' pouring the acid into the middle of the neck of the test bottle, there is also a dan- ger of complete]}' filling this with acid, in which case the plug of acid formed will be pushed over the edge of the neck by the expansion of the air in the bottle, and ma}' ))e spilled on the hands of the operator. The milk and the acid in the test bot- tle should be in two distinct layers, with- out any black por- tion of partiall}- mixed liquids be-^ tween them. Such a dark laj-er is often followed I)}' an indistinct separation of the fat in the final reading. The cause of this is possibh' that a partial mix- ture of acid and milk before the acid is diluted with the water of the milk may bring aliout too strong an action of Fia. S. The trst bottle. wrong way ol emptying pipette into the acid on the milk, and the fat in this small portion may 30 Tcs//ii slmwii that the readings oVilained in this manner gi\e coireel icsults. \\'liih3 the lower line of the fat column is iieaily stra,ight, the njjper one is curved, 77/1? Babcock Test. 33 and errors in the reading of the column are therefore easily made, unless the preceding rule is observed. The readings should he made when the fat has a temperature oi about 14tl- F.. although the results obtained will not be appreciabl_v attected if the temperature falls below 120-. The fat separated in the liabcoek test solidifies at about Kmi F. Xo read- ings should be attempted if the fat is partly solidified, as it is impossiljle to get an accurate reading in this case.* A pair of dividers will f)e found convenient for measuring the fat, and the liafiilit}" of error in reading is decreased by their use. The points of the dividers are placed at the up- per and lower limits of the fat column (from a to h in fig. 10). The dividers are now lowered, one point lieing placed at the zero mark of the scale, and the mark at which the other point touches the scale will then show the per cent, of fat in the sample tested. Yin. 10. Mea.suring the cul- uma of fat in a Baljcock test Viottle. ^- The effect of differences in the teniiierature of tlie fat on tlie readings ob- tained will be seen from the following; If 110 and 1£0'- F. be tal;en as the extreme temperatures, at which readings are roade, tliis difference of Hi'-' F. \22.o'" Cl would make a ditlerence in the volume of the lat column obtained in case of 10 per cent, milk, of ,00004 X 2 s 22.3 =- .028544 cc. or .14 per cent., .0(ii04 being the expansion coeliicient of pure butter fat per degree Centigrade between f n and 100^ C. t Zune, Analyse des Beurrns, I, ,S71, and 2, the volume of the fat in cc, contained in 17.6 cc. of 10 per cent, milk, fin 5 per cent milk tbi,^ extreme differenie would therefore be aliout 07 per cent,, or consideraOly less than one-tenth of one per cent. 34 Tcsliii!^' M/7k uiul lis Products. B. — I)iscussioN (IK TriE Details of the BAiirocK Test. 38. Although the miinipuhitions of the Babcock tost are few and comparatively simple, various difficulties ma}- be met with ill using it, particularly in the hands of beginners. The main points that have to be observed as to apparatus and testing materials in order to obtain correct and satisfac- torj' results by this test will now be considered, and such suggestions and help offered, as has been found desirable IVora an extensive experience with a great variety of milk samples, apparatus, and accessories. 1 . — Glassware. ;{!!. Test bottles. When IT.fi cc, or IS grains of milk, arc measured into the Babcock test bottle, the scale on the neck of the )>ottles shows directly the percent of fat found in the milk. The scale is graduated from U to 10 per cent. 10 per cent, of 18 grams is l.S grams. As the specific gravity of pure butter fat (i. e. its weight compared with that of an equal quantity of pure water) at the tem|)erature at which the readings are made (about 120' F,), is 0.11, l.S grams of fat will oecup}' a volume of '" = 2 cubic centimeters. The space between the and 10 per cent, marks on the necks of the test bottles must therelorc hold 2 cc, if correctly made. The scale is divided into 10 equal parts, each }>art repre- senting one |)er cent., and each of these are again sub-divid- ed into live e(|ual parts. I'lach one of the latter di\'isions therefore represents two-tcutlis of one per cent, of fat when IT.Gcc. of inilk is measured out. The small di\isions are sufficientl}' far ajjart in most Itabcock test bottles to make possible the estimation of ouc-tcuth of one per cent, of tat in the samples tested. The Bahcock Test. 35 The figures and lines of the measuring scale become in- distinct bj' use; the black color maj- be restored bj' rubbing a soft lead pencil over the scale, or by the use of a piece of burnt cork after the scale has been ruljbed with a little tallow. On wiping the necks with a cloth, or a piece of paper, the black color will show in the etchings of the glass, mak- ing these plainl}' visible. ■10. The test bottles should have a capacit}- of al:>out 50 cc, or less than two ounces; the.y should be made of well-annealed glass that will stand sudden changes of temi.ie- rature without breaking, and should be sufficiently heavy to withstand the maximum centrifugal force to which they are likely to be subjected in making tests. This force may not on the average l)e very far from 30. (55 lbs. (see 57) or the pressure exerted in whirling the bottles filled with milk and acid, in a centrifugal machine of IS inches diameter, at a speed of SiiO revolutions per minute. Special forms of test bottles used in testing cream and skim milk are described under the heads of cream- and skim milk testing. -H. Marking test bottles. Test Itottles can now be bought with a small band or portion of their neck or body ground or ''frosted," for numliering the liottles with a lead pencil. Bottles without this ground label can be roughened at any convenient spot by using a wet fine file to rub off the smooth surface of the glass. There is this objection to the latter method tliat unless carefully done, it is apt to weaken the bottles so that they will easil}' f)reak, and to both meth- ods, that the lead pencil marks made on such ground labels are easilj' erased during the test, unless the bottles are carefully handled. Small strips of tin or copper with a 36 Testing Milk and Us Prochtrls. numlier stamped thereon are sometimes attaehed as a collar aroiiiid the necks of the bottles. They are, however, easily lost, especially when the top of the bottle is slightly broken, or at an J' rate, are soon corroded so that the numbers can onl}' lie seen with difflcnltj-. The Iiest and most permanent label for test liottles is made by scratching a number with a marking diamond into the glass direct!}' aljove the scale on the neck of the Ijottles, In ordering an outfit, or test bottles alone, the operator may specify that the bottles are to l>e marked 1 to 24, or as many as are liought, and the dealer will then put the numliers on with a marking diamond. A careful record should be kept of the number of the bottle into which each particular sam- ple of milk is measured. Mistakes are often made when the operator trusts to his memory for locating the different bottles tested at the same time. 4:2. Cleaning test bottles. The fat in the neck of the test bottles must be liquid when these are cleaned. The l)ottle should lie shaken in emptying the acid, in order to remove the white residue of sulfate of lime, etc., from the bottom; if the acid is allowed to drain out of the bottle without this being shaken during the emptying, this residue will be found to stick very tenaciouslj' to the bottom in the subsequent cleaning with water. A convenient method of emptying the test l)ottles is shown in the illustration (fig. 11). After reading the fat, the bottles arc taken from the tester and placed, neck down, in the ,V inch holes of the board cover of a five-gallon stone- ware Jar. An occasional shaking while the li(iuid is running from the bottles will rinse off the precipitate of sulfate of lime. A thorough rinsing with boiling hot water by means The Bahcocl Test. 37 of an apparatus, devised I)}- one of us* (see fiii. 12) is geu- erall}- sufficient to remove all grease and dirt, as well as acid solution, from the inside of the Iwttles. When the bottles have been rinsed, the}- arc placed in an inverted po- sition to drain, on a galvanized iron rack, as shown in fig. 13, where thev are kept un- FiG. 11 Waste acid jar. til needed. The outside of the bottles should occasionally- be wiped clean and dry. 43. The amount of unseen fat that clings to glassware is general!}' not sufficient to be noticed in the results ob- tained in testing whole milk, but it plays an important part in testing samples of separator skim milk. It may be readily noticed by making a bilank test with clean water in bottles which have becQ used for testing ordinary milk, and have been cleaned by simply draining the contents and rinsing once or twice with hot water: at the conclusion of the test the operator will often find that a few drops of fat — sometimes enough to condemn a separator — will collect in the neck of the bottles, although the water tested has not been near a separator. Boiling hot water will generally clean the grease from glassware for a time, but all test bottles should, in adtlition, * l-'arriDgton. 38 Tcstin<> Milk and Its Prochicls. be given an occassional bath in some weak alkali, or other grease-dissolving solution. Persons doing consideraljle milk testing will find it of advantage to provide themselves with a small copper tank, which can be filled with a weak alkali-solution (figs. 14 and 15). After having been rinsed Flo. 12. Apparatus for cleaning test bott]e.s. A, apparatus in position; the water flows from the reservoir through the iron pipe h into the inverted test bot- tle (i through the lirass tube c, screwed into the iron pijte. j^ shows construction of tlic rutilier support on which the top of the test Ijottle rests; /, draining sink. with hot water, the test l^ottles are placed in the hot solu- tion kejjt in the tank, where thej' may be left complete!}' cov- ered with the liquid. If the tank is provided with a small The Bahcock Test. 39 faucet at the bottom, the li(iuid can be drawn otf when the test bottles are wanted. A tablespoonful of Savogran to aliout two gallons of water will make a ver^- satisfactor}- cleaning solution; sal soda, Gold Dust, Lewis li/e or Bahhitts potash are enuallj- efficient. The cleansing properties of solutions of an}- of these substances are increased by warm- ing the liquid. The test bottles must be rinsed twice with hot water after the}- are taken from this Ijath. FIg. 13. Iiraiuiug-r;irk lor test bullies. The black stains that sometimes stick to the inside of test bottles after prolonged use, can be removed with a little muriatic acid. Xi:. Pipette. The difference in the weights of various samples of normal milk generally falls within comparatively narrow limits: if a given cjuantitj' of water weighs 1 pound, the same Cjuantit}- of the usual grades of normal milk will weigh from 1.029 to 1.033 pounds, or on the average 1.03 lbs. 18 grams"'- of water measures IS cc. ; IS grams of * Cubic centimeters (abbreviated: cc.) are tbe standard used for measuring volume in tbe metric system, similar to liie quart or pint measure in our ordinary system of measures. 1 quart is equal to a little more than 1000 cubic centimeters. In tbe same way, grams represent weight, like pounds and ounces. 1 cc. of water at 4° Centigrade weighs 1 gram. 1000 grams (^ 1 kilogram) is equal to 2.2 lbs. Avoir. (See Appends, for Comparis'jns of Metric and Ctis/omary Weights and Measures). 40 Tcslino- Milk and Its Products. milk will therefore take up a smaller volume (measure less) ihau 18 cc, viz: 18 divided bj' 1.03, which is verj' nearly S^^^S2S3'\ !''■->■ This is the quantity of milk taken in the Bab- cock test. A certain amount of milk will adhere to the walls of the pipette when it is emptied, and this thin film has been found to weigh about one-tenth of a gram; conse- quentlj- 17.0 cc. has been adopted as the capaeit}' of the pip- ette used for deliv- ering 18 grams of Fig. 14. Taok lor cleaning test boUles. milk. For convenience in measuring the milk, the shape of the pipette is of importance. The mark on the stem should be two inches or more from the upper end of the pipette. The lower part should be small enough to fit looselj' into the neck of the test bottle, and not contracted 1 to a fine hole at the [xiint; the point should be large enough to allow a quick emptying of the pipette. (Fig. 1 (1). 4.>. Fool pipettes. Soon after the Babcock test A, imiiier con- 1 J. 1- 11 1 J • sfnu-tion; B, Degan to be penerallj' used at creameries as a means , . ., ^ o ^ undesirable of paving for the milk, a creamery supply house put construction. A B Fin. 16. rip- ctte points — The Bahcoch Test. 41 on the market a 20 cc. milk-measuring pipette, which was claimed to show the exact butter value of milk, instead of its content of butter fat, as in the case in using the ordinar_v 17.6 cc. pipette. A 20 cc. pipette will deliver 2.4 cc, or 13.6 per cent, more milk than a 17.6 cc. pipette, and it follows that the results obtained bymeasuring out milk for Babcock tests with these pipettes will be about 13.6 per cent, too high. In con- sidering the subject of Overrun it is noted that the excess oi butter yield over the amount of fat contained in a certain (juantitv of milk will range from about 10 to 15 per cent., or on the average 12-13 per cent. The 20 c:. pipattes may, therefore, give approxi- FiG. 15. Rack for holding test bottk-s in tank shown in fig 14. mately the yield of butter obtained from a quantity' of milk, but as will be seen, this yield is variable, according to the skill of the butter maker, and according to conditions be^-ond his control, and cannot therefore be used as a standard in the same manner as the fat content of the milk. Similar 22 cc. pipettes were also sent out. These pipettes created a great deal of confusion during the short time they were on the market, and were popularly termed "fool pipettes," as the tests obtained b_v them did not give, what they professed to do, an accurate and definite measure of the but- ter-producing qualities of different lots of milk. It is not known that any ot these pipettes are on the market at the present time. 42 Tcsl/iii'' Milk and lis Products. 4-(». Acid measures. A 17,5 cc glass cj'linder for measuring the aciil is generally included in the outfit, when a Babeoek tester is bought. This cylinder answers every purpose if only occasional tests arc made; the acid is poured into the cylinder from the acid bottle, as needed, or a quan- tity of acid sufficient for the numljer of test bottles to be whirled at a time, is poured into a, small glass beaker, pro- vided with a lip, or into a porcelain pitcher; these may be more easily handled than the heavy acid bottle, and the acid measure is then filled from such a vessel. Where a consid(n-able number of tests are made regularly, the acid can be measured into the test Ijottles faster and with less danger of spilling, I13' using some one of the many devices [proposed for this purpose. There is some objection to nearly all of these appliances, automatic pipettes, buret- tes, etc., although they will often give good satisfaction for a time while new. Sulfuric acid is so corrosive, and opera- tors as a rule take sueh poor care of such apparatus, tliat it is a very difficult matter to design a form which will remain in good working order for any length of time. Automatic pipettes attached to acid bottles or reservoirs, to jirove satisfactory, must be made entirely of glass, and strong, of simple construction, tightly closed and quickly operated. J-7. 'I'he Sviedisli Acid Bottle, answers these re()uirements better than any other j the man- ufacturers, as the operator in attemping to do so will be apt to weaken or break the bottles. Fig. 17. S\v,.(.lish acid bottle; the side-tube i.s made to hr>ld 17 .5 cc. of Calibration of glassware. 1. — Test p.oTTLES. The Babcock milk test Ijot- tles are so constructed that the scale or graduation of the neck measures a \'olume of 2 culiic centimeters, lietween the zero and the 10 per cent, marks (30). The correctness of the graduation may be easily ascertained by one of the following methods: 49. Calibration with luerciirj'. 27.18 grams of metallic mercury are weighed into the perfectly clean and diy test bottle; since the specific gravitj- of mercur}- is 13. .lO, double this quantit}' will occupj' a volume of exacth' 2 ctdjic centi- meters (44'"). The neck of the test bottle is then closed witli a small smooth and soft cork, or a wad of absorbent cotton, cut off square at one end, the stopper being pressed down to the first line of the graduation. The bottle is now ■ Maine experiment station, bull. No. 31. 44 Testinif Milk and Its Products. inverted so that the mercur}- will run into its neck. If the total space included between the (i and 10 marks is just filled with the two cubic centimeters of mercury, the grad- uation is correct. Bottles, the whole length of the scale of which vary more than two-tenths of one per cent., are inac- curate, and should not be used. The mercurj' may be convenientlj' transferred from one test bottle to another, bj' means of a thin rubljer tube which is slipped over the end of the necks of both bottles, and one weighing of mercury will thus sufflce for a number of cali- brations. In transferring the mercury, care must be taken that none of it is lost, and that small drops of mercurj' are not left sticking to the walls of the bottle emptied. A sharp tap on the Ijottle with a lead pencil will help to remove minute drops of mercurj- from the inside of it. Unless the bottles to be calibrated are thoroughlj' cleaned and drj', it is impossible to transfer all the mercury from one bottle to another. After several calibrations have been made, the mercury should be weighed again in order to make certain that none has been lost bj' the various manipulations. The scale, fig. 28, shown in (84) is sufBciently delicate for making these weighings. 50. Cleaning mercury. Even with the best of care, mer- curjr used for calibration of glassware will graduall}' become dirtj' so that it will not flow freely over a clean surface of glass. It may be cleaned from mechanical impurities, dust, films of grease, water, etc., hy filtration through heavj- filter paper. This is folded the usual way, placed in an ordinary glass funnel and its point perforated with a couple of pin holes. The mercur}- will pass through in fine streams, leav- ing the impurities on the filter paper. IMercury may Ije The Bdhcork Test. 45 freed from foreign metals, zinc, lead, etc., sometimes noticed as a grayish, thin film on its surface, liy leaving it in contact with common nitric acid for a numl.ier of hours; the mercary is best placed in a shallow porcelain or granite ware dish and the nitric acid poured over it, the dish being cov- ered to keep out dust. The acid solution is then carefully poured otf and the mercury washed with water; the latter is in turn poured otf. and the last traces of water alisorbed by means of heavy clean filter pajjer. The mercur}' to be used for calibration of glassware should be kept in a strong bottle, closed by an ordinary stopper. In handling mercury, care must be taken not to spill anj' portion of it: flnger-rings should lie remo\ed when calibra- tions with mercury are to be made. ^lercury forms the most satisfactor}' and accurate ma- terial for calibration of test bottles, on account of its heavy weight and the case with which it mav be manipulated. Equally correct results may, however, with proper care be obtained by using one of the f(>llowing methods: Calibration witli water. This maj- be done b}- means of a delicate pipette or burette, or by weighing in a somewhat similar manner, as explained in case of calil)ration with mercury. 51. ix, Measuring the icater. Fill the test bottle with water to the zero mark of the scale; remove any surplus water and dry the inside of the neck with a piece of filter paper or clean blotting paper; then measure into the bottle 2 cc. of water from an accurate pipette or a burette, divided to .>V) of a cubic centimeter. If the graduation is correct, 2 cc. will fill the neck exactly to the 10 per cent, mark of the scale. -6 Tcs/n/i;- Milk and lis Produrls. -,•> ). Wri'ihliKj the vmti'r. VWl the bottle with water tf> the zero mark ol the scale and remove anj* surplus water in the neck, as before. Weigh the bottle with the water (•(nitainecl therein. Now (ill the neck with water to the Iti per cent, mark, and weigh again. The difierence be- tween these weights should be 2 grams. In all cases when calibrations are to be made, the test l)ottlcs, or other glassware to Ije calibrated, must be thoroughly cleaned with strong sulfuric acid, or soda Ij-e, and washed repeatedl}' with pure water, and dried. Glass- ware is not clean unless water will run freely over its sur- face, without leaving any adhering drops. 53. Intermediate divisions. The space between and 10 on the scale of the Babcock test bottle is divided into 50 divisif)ns, each five of which, as previously shown (30) representing 1 per cent. Since these intermediate divisions are generally made with a dividing machine, they are as a rule correct, but it has happened that the divisions have been inaccurately placed, although the space between and 10 was correct. The accurac}' of the intermediate divisions can be ascertained b}' sliding along the scale a strip of paper upon which has been marked the space occupied bj' one per cent., and compare the space with those of each per cent, on tlie scale. 54. M. — I^jT'ETTn AN]i ACID ovLiNnEii. The pipcttc and tJie acid cylindei used in the ]{ahcock test may be calibrated by any of the methods already gi\en. Sulliciently accurate results are obtained hy weigliing the quantity of water which oacli of theses pieces of a])paratus will hold, viz: 17.0 iXi-Mus and 17.5 grains, respectively. The necessity of pre- vious thorough cleaning of the glassware is evident from Tlic Bahcock Test. 47 what has been said iii the preeediug. The pipette aud the acid measure ma}- be weighed empt}* and then again when filled with pure water to the mark, or the measureful of water ma}- be emptied into a small weighed vessel, and this weighed a seeond time. In either ease the weight of the water contained in the pipette or the acid measure is ob- tained liy difference.* Calibrations of the acid cylinder are generally not called for, except as a laboratory exercise, since small variations in the amount of acid measured out do not atlect the accu- racy of the test. 2. CEXTRlFl-(iAL ^IaCHIXES. 55. The capacity of the testing machine to be selected should l)e governed by the number of tests which are likely to be made at one time. For factory purposes a twenty-five to thirty liottle tester is generally large enough, even if toward a hundred samples of milk are to be tested at a time. The operator can use his time more economically in running a machine of this size than one holding fifty or sixty liottles; the work of filling or cleaning the fiottles and measuring the fat can be done while the centrifuge is run- ning if a double supply of bottles are at hand. Large test- ers require more power than smaller ones, and when sixty tests are completed, many of the bottles will cool, aud the fat column crystalize, before the operator has time to read them, unless special precautious are taken for keeping the bottles warm. ■*• 1 culiic ceDtimeter of dist.illed water wei^di'i 1 gram, wlieo wei.glied Id a viULium at the temperature of the maximum density of water (4- C); for the jjarposes of calibration of glassware used iu the Babcock test, sutflciently aeeurate results are, howevei-, obtained l)y weighiug the water iu the air aud at a low room temperature (60- r.i g Testing Mill^ and Its Products. 50 The tester should be securely fasteneil to a solid foundation and set so that the revolving wheel is level. The latter must l:>e carefully balanced in order that the tester may run smoothly at full speed when empty. A machine that treml)les and shakes when in motion is neither satis- factory nor safe, and the results oljtained are apt to be too low. Uiirh-standing machines are more apt to cause trou- ble in this respect than low machines, and should therefore l)e su)_)jccted to a severe test before they are accepted. If all the sockets are not filled with bottles when a test is to be made, the liottles must be placed diametrically op- posite one another so that the machine will be balanced when run. The bearings should be kept cleaned and oiled with as much care as the Ijeariugs of a cream separator. The cover of the machine should always be kept on while the l)0ttles are whirled, and should not be removed until the machine stops; the cover should be tight-fitting and may be fastened with hooks soldered on the side of the machine; test bottles have sometimes been broken while the machine has been running at full speed, and every possible precau- tion should be taken to protect the operator from any dan- ger from spilled acid or broken glass. 57. Speed required for the complete separation of the fat. There is a definite relation lietweeu the diameter of the Jialicock testers and the speed required for a perfect .seiiaration of the fat. In tiie preliminary work with the lialicock test tlie inventor found that with the machine used, the wheel of which had a diameter of eighteen inches, it was necessary to turn the crank, so as to give the test bot- tles seven (o eight hundred revolutions per minute, in order to effect a maximum sejiaration of fat; later work has The Bahcock Test. 49 shown that this speed is ample. Taking therefore this as a standard, the centrifugal force to which the con- tents of the test bottles arc snbjected when supported on an eighteen inch wheel and turned SOO revolutions per minute, can be calculated as follows : The centrifugal force, F, acting on the bottles is expressed by the formula T^ \v. v'^ Il) 32. 2r in which w = tlie weight of the bottle with contents, in pounds ; V ^ the velocitj", in feet per second, and r ^ the radius of the wheel, in feet. When tlie wheel is turned SOO times a minute, a bottle supported on its rim will travel 2 ;rr X '',.",;' = 2 X 3.14f 5 X /'. X "«"„" = 62.83 feet per second. The weight of a bottle, with milk and acid, is verj' near 3 ounces, or j'",, of a pound. Substituting these values for V and w, gives F= 1 « A ^^-^■?^-> =30.65 lbs. •J x'n 1 2 The bottles are therefore, under conditions given, subjected to a pressure of 30.65 lbs. fn order to calculate the speed rerjuired for obtaining this force in case of machines of other diameters, the value of r in formula ( f ) is found from \v Substituting the values for F and w. ♦ 32^2^211 . . . (H) i/32.2 X 30.65r v= V^--^X^"-0^r _ -^/^2647 I'l: In this equation the values r ^ 5, 6, 7, . . . 12 inches are substituted in each case (/'j, i",, i^, ■ • • || feet), and the velo- city in feet per second then found at which the bottles are whirled when placed in wheels of diameters 10 to 24 inches, and subjected in each case to a centrifugal force of 30.65 lbs. As the number of 60 F revolutions per minute ^ -r, , v being as before the velo- 4 c;,) 7^cs//i/l; Mill- (I lid iLs Products. city in fccl |.cr secoii.l, .-md r llir r;ulius of the wheel, the speed at which the wheel iinisl lie tiinieil.is l.iund by suljstitiitiiiK for v the V iliMS olil Mined in tlie ]ireee(lin^{ ealenhilions in case of wheels of (linirenl iliMnieters. Tlie results of these ealeulations are given in the lollowiii^j, tlilile; !)i,,inr/'r }'tfhir!li/ In feet Numhp.r of rtv(diUiOfis e turned ',\''=(;.'l (.see tabltO, fir al)out once every second, in order to effect a maximum separation of fat. liy counting the num- lier of n^volulions, watch in hand, and consulting the pre- ceding talih', the operator will soon note the speed which mn.st be maintained in case (ifliis jiarticiilar machine. It is vitally imi)ortaijt that the required speed is always kept up; if MiroM-;li carelessnes.s, worn-out or dry bearings, slipping b(dt.s, etc., the .speed is slackened, tlie result will come too The Bahcoch Test. 51 low, it ma}' be a few tenths, or even more than one percent. Care as to this point is so much the more essential as the results obtained bj' too slow whirling may seem to lie all right, a clear separation of fat being often olitaincd even when the fat is not completely separated. 59. Ascertaining the necessary speed of testers. In buj'ing a tester the operator should first of all satisfy himself at what speed the machine must be run to give correct results ; the preceding taljle will serve as a guide on this point. He should measure out a dozen tests of the same sample of millv, and whirl half the number at the speed required for machines of the diameter of his tester. Whirl the other half at a somewhat higher speed. If the averages of the two sets of determinations are the same, within the probable error of the test (sa^-, less than one-tenth of one percent.) the first whirling was sufficient, as it is believed will gener- allj- be the case. If the second set of determinations come higher than the first set, the first whirling was too slow, and a new series of tests of the same sample of milk should be made to ascertain that the second whirling was ample. This method will test not only the speed required with the particular machine at hand, but will also serve to indi- cate the correctness of the calibration of the bottles. A large numlier of tests of the same sample of milk made as directed (pouring the milk once or twice pre\'ious to taking out a pipetteful for each test) should not vary more than three-tenths of one percent, at the outside, and in the hands of a .skilled operator will come within two-tenths of one per- cent. If greater discrepancies occur, the test bottles giving too high or too low results should be further examined, and calibrated according to the directions already given (41)). Tcsfiiio- Jlli'Ik and Its Products. (>(). Hand testers. When only a few tests are made at a time, and at irregular intervals, as in case of dairj'men who test single cows in their herds, a small hand tester answers every purpose. These may lie had in sizes from two to twelve bottles. In selecting a particular make of tester the dairj'men has the choice of a large number of different kinds of machines. It is a source of regret that most of the machines placed on the market for this purpose in the past have been so cheaplj' and poorlj* constructed as to prove very unsatisfactory after having been in use for a time. The sharp competition between manufacturers of dair}- supplies, and the clamor of dairymen for something cheap, full}' account for this condition of affairs. This ap- plies especially to the many machines made with belts or friction application of power. The main objection to these machines is the uncertaint}' of the speed obtained, when the}' have been in use for some time, and the belt or friction appliance begins to slip. Hand testers made with cog gear wheels are more to be depended on for giving the necessary speed than belt or friction machines; thej' are generally noisj', but the bottles arc always whirled at the speed which the number of turns made bj' the crank would indicate. Fig. 18. " No-tin '' test. The Babcock Test. S3 The " No-tin" test (see fig. 18) is, in the opinion of the authors, worthy of special mention among the hand testers made at the present time; it is a six-bottle geared machine, durable of construction, and runs smoothly and without noise. ()1. Power testers. For factory purposes, steam tur- bine machines (fig. I'J) are inost satisfactorj' when well made and well cared for. The}' should alwaj's be provided with a speed indicator and steam gauge, both for the purpose of knowing that sufficient speed is attained, and also to pre- vent wliat ma}' lie serious accidents from a general smash- up, if the turljine " runs wild " by turning on too much steam. The revolving wheel of the tester should be made of wrought or malleable iron, or of wire, so that it will not be broken by the centrifu- gal force, thus avoiding seri- ous accidents. The swinging pockets which hold the test bottles in some machines, should be so made that the bottles will not strike the center of the revolving frame when in a horizontal position. Tests have often been lost by the end of the neck catching at the center, the bottles thus failing to take an upright position when the whirling stops. Fig. 19. Type of Babcock steam turljine testers. 54 Testing Milk and Its Products. 3. — Sulfuric Aoid. 62. The sulfuric acid to be used in the JJahcock test should have a specific gravity of ] .82-1. S3."'' The com- mercial oil of vitriol which can be bought for about 2 cents a pound in carboy lots, is commonly used. One pound of acid is sufHcient for fifteen tests. The acid should be kcfit in stoppered glass bottles, preferably glass or rub- ber stoppered ones, since a cork stopper is soon dissolved by the acid and rendered useless. If the bottle is left un- corked, the acid will absorb moisture from the air and will after a time become too weak for use in this test. Lead is the onl}' common metal which is not dissolved by strong sulfuric acid; where considerable milk testing is done, it is therefore desirable to provide a table covered with sheet lead on which the acid may be handled. 63. The acid dissolves iron, tin, wood and cloth, and burns the skin. If acid is accidently spilled, plenty of water should be used at once to wash it off. Ashes, potash, soda, and ammonia neutralize the action of the acid, and a weak solution of any one of these alkalies can be used after the acid has been washed otT with water. The red color caused by the action of the acid on clothing can be removed by wetting the spot witli weak ammonia water; the ammonia must, however, be ai)plied while the stain is fresh, and is in its turn washed off with water. 6+. Testing the strength of the acid. The strength of the acid can be easily tested l)y the use of such a balance as shown in fig. 27 (SI). A dry test bottle is weighed, and *AHp(.'cinc gravity of 1,82 nieaos that aoy given volume of the acid weighs 1.82 times as much as tlie same volume of water at the same temperature (see also under Lactometer, !'4). The Bahcoch Test. =;5 then filled with ncid exactly to the zero mark, or to an}- other particular line of the scale. It is then again weighed aecurate- !}•; the difl'erence between these two weights will gi\-e the weight of the acid in the bottle. Xext emptj' the bottle and rinse it thoroughlj- with water (until the water has no longer an acid taste) ; fill the bottle with water to the same line as be- fore and weigh; the difl'erence l)etween this weight and that of the empt_y bottle gives the weight of the same ^'ohimo of water as that of the acid weighed. Divide the weight ol the acid by the weight of the water; the quotient gives the spe- cific gravity of the acid. If this is between 1.S2 and L.^o, the strengtli of the acid is correct. The outside of the test bottle should always be wiped dry before the li(iuids are weighed in it. Unless great care is taken in measuring out the acid and the water, and in weighing both these and the test l)ottle, the results o))tained will not he trustworthj'. 05. Too strong acid can sometimes be successfully used bj' taking less than the re(|uired amount of each test, e. g. about 15 cc. (Operators are warned against reducing the strength of the acid l)y adding water to it, as accidents are very apt to occur when this is done. A too strong acid can, if desired, be weakened by simplying leaving the bottle which holds it, uncorked for a time; or b}- pouring the acid into a bottle containing a small quantity of water; in the latter case, the first portions of acid should be added care- fully, a little at a time, shaking the bottle after each addi- tion, so as not to cause it to l.)reak from the great heat evolved Iq mixing the acid and the water. Never dilute acid hy pouring water into it. 66. If tlie acid is too weak, correct results may some- times be ol)tained by using more than the specified quantity. S6 Tesiinor Milk mid Its Products. sa}- 20 cc. If a good test is not obtained with tiiis quantity of acid, a new lot must be secured, as its specific gravity in such a case must be below 1.82. The observing operator will soon be able to judge of the strength of the acid by its action on milk in mixing the two liquids in the Babcock test bottles; it is indeed remarkable what slight differences in the specific gravity of the acid will make themselves apparent in working the test, as regards the rapiditj' with which both the curdled milk is dissolved, and the mixture of acid and milk turns black. 67. The relation between the strength of sulfuric acid and its specific gravity will be seen from the following table: Strength of Sulfuric Acid {Lunge and Isler, 1800). Stdjurlc Acid 97 per cent. 96 95 94 93 92 91 90 89 Specific Gravity (1.5° C, water i° C). 1.841 1.840 1.839 1.837 1.834 1.830 1.825 1.820 1.815 1.808 It will be noticed that the sulfuric acid to be used in the Babcock test should contain 90 to 92 per cent, of acid (II.jSOj); slightly weaker or stronger acid than this may, as previously stated, lie used )iy adjusting the quantity of acid taken I'or each test to the strength of the acid, but success- ful tests cannot be made with acid weaker than 80 per cent., or stronger than 05 per cent. The Babcoch Test. 57 f-S (>(S. The Swedish acid tester (fig. 20) is a small h}-- drometer, intended to show whether the acid to be used in the IJabcock test is of the correct strength. "We have examined a number of these testers, and have found them practicallj- useless for the purpose in- tended. The reason for this lies in insufficient sensitiveness in the instrument; while the testers examined were found to sink to the line marked Correct on the scale, when lowered into sulfuric acid of a specific gravity' of I.80, they would sink to a ( ; \ I point much nearer the same mark when lowered ^^*9 into either too strong or too weak acid, than to the '' lines marked Too strong or Too tceak. respectivelj'. An examination of the proportionate parts of the testers shows that such must be the case: The total weight of the testers varies between 7 and 8 grams ; the diameter ot the stem is nearly 5 millimeters, and the dis- tance between the two lines marked Too strong and Too weak is 13.5 millimeter. A good hydrometer, such as used in chemical laboratories for determining the spe- FlG '^0 . - - . . , o ',. ,' cine gravity ot lirruids of l.S to 2.(1, weighs about 75 Swedish .-^ - L 1 <-i acid tester, grams; the diameter of the stem is 6 mm., and the dis- tance between the 1.S2 and l.S-t marks on the scale is 15.5 mm.; these limits ma3' be taken to represent too weak and too strong acid, respectivelv. Comparying now this hydrometer with the Swedish tester, the weight of the for- mer would make it ten times as sensitive as the latter, if the size of the stem was the same in either case; as it is, the tester has the advantage in point of thinness of stem I see 94), as the volumes of the same lengths of stem in the two instruments aie as the square of their diameters, i. e., as 25:36. This meansthatthc Swedish tes- ters are onlv = as sensitive asthe hydrometer, or a ■ 10 X 25 7 ■difference on the scale of the latter amounting to 15.5 mm. (see 58 Testing Alilk and lis Producls. above), would represent only 2.2 mm., on the scale of the Swedish tester. The line marked Too strong must therefore be only 1.1 mm. ( .}.j of an inch) below the Correct line; and that marked Too weak the same distance above the line. But this is too small a distance to be differentiated Ijy persons unfamiliar with the use of delicate hydrometers, especially since the meniscus of the licpiid formed around the stem of the tester renders an accurate reading somewhat difficult. The Swedish acid tester can be made more delicate by changes in one or two directions: b}' making the bulb larger, thus necessi- tating an increase in weight, or by making the stem thinner. Viy way of comparison it maj-be stated thatthe hydrometers used for determining the specific gravity of the ether-fat solution in Sox- hlet's areomcLrie method of milk analysis have a stem only 2 mm. in diameter, and the distance of the scale between. 705 and .~i7> is 70 mm., or 2'"'i. inches. Even if these testers are changed as suggested, their practicabil- ity still remains an open question. The action of sulfuric acid of difterent strength is very characteristic (GG), and in the hands of experienced operators, is as delicate an index to the strength of the acid as can be desired, making rather unnecessary a separate instrument for ascertaining the correctness of the strength of the acid used in inilk testing. (j!(. The color of the fat column an index to the strength of the acid used. The strength of the acid is indicated to a certain extent l:)y the color of the fat which separates in the neck of the test bottle when ntilk is tested. If the directions given for making the test arc care- full}' followed, the fat separated out will be of a golden yellow color. It the fat is liglit colored or whitish, it gen- erall}' indicates that the acid is too weak, and a dark colored fat, with a layer of Ijlack material beneath it, shows that the acid is too strong. The strength of the acid used in the test is not sullicient at ordinary temperatures of testing to appreciablj- dissolve TIic Babcoch Test. 59 the fat, but a variation in the strength of the aeiJ or in the temperature of the millc iiilhiences the intensity of the ac- tion of the acid on the fat, as shown in the color of the fat obtained. The following experiment shows the relation between the strength of the acid, the temperature of the milk, and the color of the fat. First: — From a sample of milk measure the usual (|uantit_\' for testing into each of three bottles, A, B and C. I-'lace A in ice v/ater, and C in warm water, leaving bottle B at the ordinary temperature. After the Ijottles have been left for twentv minutes under these conditions, add the normal quantity of acid to each and proceed with the test in the ordinary manner. Second: — Measure some of the same milk into three otlier Ijfit- tles, D, E and P. Into test bottle D pour the usual amount of rather weak acid; add the same amount of acid of normal strength ( 1 .82-1.93) to bottle E, and add 17.5 cc. of a still stronger acid, (concentrated sulfuric acid, sp. gr. 1.84) in test bottle F; complete these tests in the usual way. On the completion of the preceding six tests the operator will notice that the fat in the necks of test bottles A {cold milli) and D ( weak ac^'d I is much lighter colored than that in C I warm mill; ) and F {strong acid), and that the color of the fat in B {normal temperature] and E { normal acid) is somewhere between that of these two series. 70. Influence of temperature on the separation of fat. The intensit}^ of the action of the sulfuric acid on the milk is influenced bj* the temperature of either liquid; the higher the temperature, the more intense will be the action of the acid on the solids of the milk. It ma}- be noticed that acid from the same carboy will act differently on milk in summer than in winter time, if the acid and the milk are not brought to the same temperature before testing during 6o Testintr Milk and lis Products. both seasons, by cooling or heating, respectively. The temperature of the liquids may be as low as 40'' F. in winter and as high as SO"" F. in summer. This difference of forty degrees will often have considerable influence on the clear- ness of the fat separated, showing white curdy substances, and a light colored fat in winter, and black flocculent specks, with a dark colored column of fat in summer. Both these defects can be avoided when the acid is of the proper strength, by -bringing the temperature of the milk and the acid to a):iout 70^ F. before the milk is tested. The operator should be particularly cautious against over- heating either milk or acid; so intense an action ma}^ be caused thereby as to force the hot acid out of the neck of the test bottle when it is added to the milk, thus spoiling the test and possiblj- causing an accident. 4. — Water to be Used in the Babcock Test. 71. Rain water, condensed steam, or soft water should be used for the purpose of bringing the fat into the neck of the test bottles. The surface of the fat column will then usually be clear and distinct. The foam or IniliViles that sometimes obscure the upper line (meniscus) of the fat, making the point indistinct from which to measure it, is gen- erally caused by the action of the acid on the carbonates in hard water. The carbonic acid gas liberated from hard water by the sulfuric acid is more or less held by the viscid fat and produces a layer of foam on its surface. If clean soft water cannot be obtained for this purpose, hard water ma}' be used ))y adding a few drops of sulfuric acid to the water before it is heated, thus causing the carbonic acid to be driven out of it. By simply boiling, most hard waters will be rendered soft, and adapted to use in the Babcock The Babcock Test. 6i test, as the carbonates which cause this foaming are thereby precipitated. If the test has been completed, and a la3'er of foam ap- pears over tlie fat, it may be removed by adding a drop or two of alcohol which will destroy the foam. If this is done, the fat column should be read as rapidly as possible after the alcohol is added, and before the latter unites with the fat and increases its volume. Fig. 21. The Russian test. 73. Reservoir for water. When only a few tests are made at one time, the hot water can be added with the 17.6 cc. pipette. If many tests are made, the water is more con- 62 Tcstino- Milk and lis Produrls. venicntly and qiiicklj' flUeil into the test })ott]e.s by drawing it from a small copper reservoir or tin pail suspended over the testing machine.* The How of water through a rubber tube connected with the reservoir, is regulated by means of a pinch cock. The water must ))e hot when added to the test bottles so as to keep the fat in a melted condition until the readings are taken. The use of zinc or steel oilers, or perfection oil cans has been suggested, as a handy and rapid method of adding hot water to the test bottles. 5. IMODIFICATIO.N'S OF THE BaKCOOK TeST. 73. The Russian milk test. The same chemical and mechanical principles applied in the regular Babcock test, A) are used in the Russian milk test, ex- // cept that in this case the machine in // which the bottles are whirled, and // the bottles themselves, are so con- H-3 // structed that the latter can lie filled with hot water while the machine is running at full speed, thus saving time and trouldc incident to the stopping of tlie tester and filling the bottles bj- means of a pipette. The milk-measuring pipette (fig. 22.) and the acid measure used in the Russian test are one-half the ordinary size, and the test bottles are made in two pieces, with a detacliable narrow graduated stem (see fig. 2!!). The machine is substantiallj' made of cast iron; it is provided with a very satisfactory speed indi- cator which shows at any time the number of FiQ. 22. Pip- revolutions at which the l)ottles are being turned. ette used in Uie rm - •^■^ , ,- i , i Eaisian test The accompanying illustrations show the appa- * Ordinary tinware rnnts very soon wlnni water is left standing in it, and cop- per re8ervoir.s are tliercforo more ocononiieal. Tlic Babcocl- Test. 63 n ratus used in the Russian test. In our experience witli tliis maehiue there seemed to be a tendency toward too low results. 7-t. Bartlett's modification. Bartlett ' proposed a modilication of the method of procedure in the Babcock f-="-^ test, which aims to simplif}- the manipu- lations. '!{) cc. of acid are added, instead of 17.5 cc, and the bottles filled with the milk-acid mixture are left standing for not less than five minutes and then filled to within the scale, with hot water; the l^ottles are then whirled for five minutes at the regular rate (4S). In the experience of the authors the modification cannot alwa3S lie depended upon to give satisf actor}' results. It was tried by each of the one hundred students in the Wisconsin Dairy School during 1890-07; while some of these operators oljtained a clear separation of fat, and results that compared favorablj' with those made b}- the regular Bal)cock test, others failed to ol)tain correct results with the method as modified. It is not known that the modification has proved superior to, or taken the place of the regular Babcock test to anj- extent. >■ ]"].;. 23 Test lioltle: used in the Russian test ' ]Maine experiment station, bull. No. r;i, (S. S.) 64 Tcstino- Milk and /Is Proditcls. CHAPTER IV. CREAM TESTING. 75. Cream may be tested by the Balicock test in the same manner as milk, and the results obtained are accurate Fig. 24. Students when the necessary care has been taken in sampling the cream and in measuring the fat. The composition of cream varies greatly according to the process of creaming, tem- perature of milk during the creaming, quality and composi- tion of the milk to be creamed, etc. The cream usually met with iu the creameries or on the market will contain from Cream Testing: 65 15 to 30 per cent, of fat; during late j'ears the practice in man_y creameries has been to churn a very rich cream, containing from 30 to 40 per cent, of fat. Such cream is, however, rarely' met with, and cream containing 25 per cent, of fat may be considered of average composition tox a good quality of cream.* If 1 8 grams of such cream is measured into an ordinary Babcock test bottle, it follows that there will be IS X .25 = 4.5 grams (or ir* = 5 cc.) of pure butter fat in the bottle. It is shown, however, (p. 34), that the space from to 10 in the neck of these bottles holds exactly 2 cc. The neck of the milk test Ijottles will not therefore lie large enough to show the per cent, of fat in a sample of cream if IS grams are taken for testing, so that less cream must be measured out, or special forms of test bottles used (70). 76. Errors of measuring cream. Several factors tend to render inaccurate the measuring of cream for the Babcock test, and in exact work, it is recommended to weigh the amount taken for a test. If a 17. G cc. pipette is used for measuring the cream, it will not deliver 18 grams of cream, as it will of milk, for the following reasons: 1. The specific gravitj' of cream is lower than that of milk; if a certain quantity of milk weighs 1030 lbs., the same C[uantity of cream will weigh from 1020 lbs. to below 1000 lbs., the weight being determined hy the richness of the cream; the more fat the cream contains, the less a certain quantity of it, e. g., a gallon, will weigh. t 2. Cream is thicker (more viscous) than milk at the same temperatures, and more of it will adhere to the sides of the measuring pipette than in case of milk. This is of special importance in testing ver}' rich or sour cream. * For average quality of cream fumisheii at five Connecticut creameries 3ee (194). f For specific quantity of cream of different richness, see table on p . G6. 66 Tcsiivi^ Alilk and Its. Products. 3. In case of separator cream, more or less air will become incorporated with the cream during tiie process of separa- tion. In the ripening of cream, fermentation gases are de- veloped and held in the cream in the same wa}' as bread dough holds the gases generated by the j'east. In either case the weight of a certain measure of cream is diminished. 77. As an illustration of the effect of the preceding fac- tors on the amount of cream measured out b}' a Babcock 17.6 cc. pipette, the following weighings of separator cream are given. The cream was in all cases fresh from the sep- arator; it was weighed as delivered by the pipette into a Winton cream bottle (81), and the test proceeded with at once. The specific gravitj^ of the cream measured out was determined by means of a picnometer. The data given are in all cases averages of several determinations; the samples of cream have been grouped according to their average fat contents.* Weight of cream delivered hi/ a 17,(1 cc. pipette. Per ci. of fat in cream Specific gravily (17. -5° C.) Weight, of cream delirered, grams- 10 1.023 17.9 15 1,012 17,7 20 1.008 17„S 25 1.002 17.2 30 .996 17.0 35 .980 16,4 40 .966 16.3 45 .950 16,2 50 ,947 15.8 The data given in the table show plainly the variations in the specific gravity of cream of different richness and the * For iofluenee of condition of cream on the amount measured out with a 17.6 cc, pipette, aee alao Ijartlett, Maine exp, sta,, buil, .'il (a, 8,) Cream Testing-. 67 error of roaking tests of cream b)' measuring it ivith a 17.6 cc. pipette; if tiie cream to be sampled is fresh separator cream, testing over 30 per cent., less tlian 17.0 grams of cream will lie delivered into tire test bottle, and the results of the reading will be at least one- eighteenth too low, or about 1.4 per cent, on a 25 per cent, cream. If the cream is sour, the error will of course be still greater. 7 S.^ Avoiding errors of measuring cream. The pre- ceding table shows that a 17.0 cc. pipelte in case of cream containing less than 25 per cent, of fat, and fresh from the separator, will deliver onlj- about 17.2 grams of cream; it is therefore evident that the pipette to deliver cream for the Babcock test must be made larger than the \1A> cc. p.iipette used in testing milk. Quite satifactory results maj- be ob- tained in testing such cream, by using a is cc, measuring pipette; to avoid the expense and trouble of using two dif- ferent pipettes, one for milk and one for cream, a pipette with two marks on the stem, at 17. C cc. and at 18 cc, has been placed on the market, the former mark lieing used when milk is tested, and the latter for cream. In testing cream b}' the Babcock test, one of two methods may be followed: First, one of the special forms of cream test bottles which have been devised is used; or. Second, only sufBcient cream to be tested in a regular Babcock milk test bottle is taken for a sample. 79. Cream test bottles. Three special forms of bot- tles have been devised for testing samples of cream hy the Babcock test; two of these were suggested by Bartlett of !Maine,* in 1892; one with a long detachable neck designed - iMaine experiment station, Ijulletins 3 and 4 (second series). 6S Tcsliiiij- Milk (Did Its Products. for testing very rich cream (up to 35 per cent, fat), and the other with a neck wid- ened into a bulb in the mi(hlle so as to allow a large quantity of fat to be meas- ured. The former kind of cream bottle has, so far as is known to the writers, found liut a limited distribution; the neck is too long to be used in the ordinary centrifugal machines, and is not attached until the base portion, containing the cream, acid and first filling with water, has been whirled. This cream bottle is more difficult to handle and cannot be considered as practical as either of the two other forms of bottles devised for testing cream. (SO. The Jjulh-neclct^d cream buttles, (fig. 25), allow the testing of cream containing liri or 25 per cent, of fat, the usual fjuantity of cream (LS grams) being measured out. The neck is graduated from (I to 23 per cent., and in some cases to 25 per cent, the graduation extending both below and above the bull). This is sometimes an in- conA'cnience, as the water must be added carefully so that tlu^ lower end of the col- umn of fat will always come below the bull), in the graduated })artof the neck, and not in the bulb itself l<]specially in case of beginners, tests are often lost when this bottle is first used, for the reason given, uiiUl the ojjcrator learns to add the proper amount of hot Fig. 2."., Tliu liu necked cream t liciltlc. Cream Tcsti no: 69 water to float the fat to some point within the scale. It is recommended to fill those bottles with the first portion of hot water to jnst above the buli), so that one can see how much water to add the second time in order to bring the fat within the scale. Each division of the scale on these cream bottles represents two-tenths of one per cent, of fat, as in case of the milk test bottles. 81. The ^Yinton cream hottle. Tlie cream test bottle devised by Winton.'' (fig. 20), has a neck of the usual length, and suffl- cientlj' wide to measure ?>i) per cent, of fat. The scale of the neck is divided into one- half percents, but readings of a quarter of a percent can easily be estimated. De- terminations of fat in cream accurate to a quarter of a percent are sufflcieutl}' exact for most commercial purposes, e. g., in creameries, and this form of cream Ijottle will be found very convenient in making tests of composite samples of cream. 82- Use of milk test bottle. Cream maj' be tested by emptying a 17.(1 cc. pip- etteful of the sample into two or more test bottles, dividing the amount about equally between the bottles, and filling the pipette with water once or twice, which is then in turn divided about equally between the test bottles; the Fis. 26. The Win ton cream bottle. Mi'onnecticut experiment station (New Haven), bull. No. 117; report ISCU, p. 224. 7o Testing Milk and Its Products. per cent of fat in the cream is found by adding the readings obtained in each of the Ijottles. This method does awaj' with the error incident to the adhesion of cream to the side of the pipette, but not that due to the low speci- fic gravitj' of the cream, and the results obtained will there- fore be somewhat too low. The dilution of the cream with water in the test bottles not oul}- makes it possible to bring into the bottle all the cream measured out, but also insures a clear test. If ordinarj' cream is mixed with the usual quantity of sulfuric acid used in the I'abcock test, a dark- colored fat will generallj- lae obtained, while the cream diluted with an equal or twice its volume of water, when mixed with the ordinary amount of acid, will give a light yellow, clear column of fat, which will allow of a very dis- tinct and sharp reading. The number of bottles to be used for testing a sample of cream by this method must be regulated by the richness of the cream. If a sample probabl}' contains 20 per cent, or more, one pipetteful should be divided nearlj' equallj' be- tween three milk test bottles, and two-thirds of a pipetteful of water is added to each bottle. If the cream contains less than 20 per cent, of fat, it will only be necessarj' to use two milk test bottles, dividing the pipetteful between these, and adding one-half of a pipetteful of water to each bottle. By using cream test bottles (70), more accurate tests may be obtained, in case of cream containing as much as 25 per cent, of fat, by dividing one i)ipetteful between two bottles, rinsing half a pipette of water into each one, than b}' add- ing all the cream to one bottle, without rinsing the pipette, for reasons apparent from what has been said in the pre- ceding. Cream Testini 71 83. Use of 5 cc. pipette. When the cream is in good condition lor sampling, satisfactory- results can be obtained by tlie use of a 5 cc. pipette; 5 cc, of cream are measured into a milk test bottle, and two pipettefuls of water are added. In this wa}' all the cream in the pipette is easily rinsed into the test bottle. The readings multiplied by li ^ :i.G will give the per cent, of fat in the cream. If the specific gravity of the cream tested varies appreciabl}^ from 1, corrections should be made accordingly; e. g,. if the specific gravity is 1.02, the factor should read _■!_ = 3.63; if .05, - ''- = 3.79, etc. S-l:. Weighing the cream. For the reasons already given, it is always to l)e preferred to weigh the cream into the test bottles when accurate tests are required. When a small delicate balance is used, this can be done quite rap- idly. Either of the scales shown in the accompanying illus- tration (figs. 27 and 28), will be found useful and sufliciently accurate for this purpose; a small scale of this kind is also FKt 27 S( ale used for weighini; cream, cheese, etc., in the Babcock test. Fig. 2>!. Torsion balance used for weighing cream, cheese, etc., in the Babco'.'k test. convenient and helpful in testing cheese, butter and con- densed milk, and in determining the strength of sulfuric acid, and the accurac}* of test bottles and pipettes (q. v.). In test- ing cream bv weight, the test bottle is first weighed empty, 72 Testing j\IUk and lis Products. and again when 5 to 10 cc. of cream have been measured into it; the difference between the two weights gives the weight of cream taken for the test. If the cream contains less than 30 per cent, of fat, the regular milk test bottle can be used for testing the cream, if not much more than 5 grams are weighed out; if more cream is taken, or if this is richer than 30 per cent., it is advisable to use the bulb-necked cream bottle. The operator should be careful in weighing the cream not to spill it on the outside of the test bottle, as the Ijal- ance does not discriminate between cream inside and out- side of the bottle. Snflicient water is added to the Ijottle to make the total volume al)Out 15 cc. The usual quantity of acid (17.5 cc.) is then added, and the test completed in the ordinary manner. The reading of the amount of fat in the neck of the test bottle in this case does not show the correct per cent, of fat in the cream, because less than 18 grams were weighed out. The per cent, of fat in the cream tested is obtained b}' multiplying the reading bj* 1^, and dividing the product b}' the weight of the cream taken. Example: Weight of cream tested, .5.2 grams; reading of col- umns of fat "4-. 8, '>4.7, average 4.75; per cent, of fat in the cream. 1:15X18=16.44. 5.2 The weighing of cream and the reading of the fat column must Ije made very carefuUj'; a division of one-tenth on the neck of the test bottle has a value of over three-tenths of one per cent, of fat when 5 grams of cream are tested, and six-tenths of one per cent, if only 3 grams of cream are weighed out. The reading is rendered more accurate and certain if a number of tests of a sample are made, at least two or three, and the results averaged. Cream TestiiiQ-. n The accompanying illustration, (fig. 29). shows the proper method of reading the fat column in cream tests; readings are made from a to h, not to d or to c. 85. No special precautions other than those required in testing milk have been found necessarj- in testing cream, except that it is sometimes advisable not to whirl the test bottles in the centrifuge at once after mixing, but to let the cream- acid mixture stand for a while, until it ^ — d turns dark colored. At first, the mixture of cream and acid is much lighter colored than that of milk and acid, owing to the smaller amount of solids not fat contained in the cream. The liquid Ijeneath the fat in a completed test of cream is sometimes milkj', and the fat appears white and cloud}', making an exact reading diflfcult. Such defects can usually be overcome by placing the test bottles in hot water for about 15 minutes previous to the whirling, or by allowing Fig. 29. Measuring ,, ^ . , t ii' / i ■ i • i i tbe fat coiumii in the the fat to Crystallize (which is done by neck of a cream bot- i- ., , iii ■ • . lit tie. ueadings should cooling the bottles in ice water or cold water be made from « to 6, not to (J or to c. after the last whirling) and remelting it by placing the bottles in hot water. 74 Tcstiiii^- Milk and Its Prochicls. CHAPTER V. BABCOCK TEST FOR OTHER MILK PRODUCTS. S(). Skim milk, butter milk, and whey. Each di- vision on tlie scale of tlie neck of the regular Babcoek test bottle represents two-tenths of one per cent. (31()- When a sample of skim milk or butter milk containing less than this per cent, of fat, is tested, the estimated amount is ex- pressed b_y different operators as one-tenth, a trace, one- tenth trace, or one to five hundreths of one per cent. Gravi- metric chemical analj'ses of skim milk have shown tiiat samjjles which give only a few small drops of fat floating on the water in the neck of the test bottle, or adhering to the side of the neck, generallj' contain one-tenth of one per cent, of fat, and often more. Samples of skim milk containing less than one-tenth of a per cent, of fat are very rare, and it is douljtful whether a sample of separator skim milk representing a full run of, saj' 5000 lbs. of milk has ever shown less than flve-hundreths of one per cent, of fat. Under ordinary factory conditions, few separators will deliver skim milk containing under one-tenth of one per cent, of fat, when the sample is taken from a whole day's run. This must be considered a most satisfactory separation.'-" 87. The reason wh^' the Bal)C0ck test fails to show all the fat present in skim milk must be sought in one or two <= IVjr oompiiraLive analypca of separator akim mUk by the gravimetric metliod aod by the Babcoek test, see bull. 52, Wis. exp. station. Bahcock Test for Other Milk Products. /D causes: a trace of fat ma_y lie dissolved in tlie sulfuric acid, or owing to the minuteness of the fat globules of such milk they ma}- not lie brought together in the neck of the bottles at the speed used with Babcock test. If a drop of the dark liquid obtained in a Babcock bottle from a test of whole milk, Ije placed on a slide under the microscope, it will be seen that a fair number of ver}- minute fat globules are found in the liquiil. These globules are not brought into the column of fat in the neck of the bottle by the cen- trifugal force exerted in the Babcock test; the loss of the fat contained in these fine globules is compensated for, in the testing of whole milk, by a liberal reading of the column of fat separated out, the reading being taken from the lower meniscus of the fat to the top of the upper one (see p. 32); in some se])arator skim milk, on the other hand, not enough fat remains to completel}' fill the neck, and the apparent result of the reading must there- fore be increased by from flve-huudredths to one-tenth of one per cent. It follows from what has been said that tests of skim milk showing no fat in the neck of the test bottles on completion of the test, generall}- indicate inefficient work of the centri- fugal tester, or the operator, or both. The test should be repeated in such cases, using more acid and whirling for full four minutes. In order to Ijring as much fat as possible into the neck of the bottles, in testing skim milk, it is advisable to add some- what more acid than when whole milk is tested, viz: about 20 cc, and to whirl the bottles at full speed for four to five minutes. The readings must be taken as soon as the whirl- ing is completed, as owing to the contraction of the liquid by cooling, the fat is otherwise spread over the inside of Icstino; Milk and lis Products. the neck of the test bottle as a film of grease which cannot be measured by the scale. 8S. The douhle-neched test bottle, (fig. HO), suggested by one of us,* is made especiall}- for measuring small quanti- ties of fat and gives most satisfactory re- sults in testing skim milk and butter milk. Each division of the scale in these bottles represents five-hundredths of one per cent., and the marks are so far apart that the small fat column can be easily estimated to single hundredths of one per cent. In the first forms, now not in common use. the neck was graduated to hundredths of one per cent. The value of the divisions of the scale on the double-necked test bottles has been dis- cussed of late in dairy papers, and various opinions have been expressed whether thej' show one-tenth or one-twentieth (.05) of one per cent, of fat. I5y calibration with mercury the value of the divisions will be found to be .05 or one-twentieth of one per cent., but, as shown above, the results obtained in using the bottles for thin separator skim milk come .05 to .1 per cent, too low, so that, practicallj- speaking, each division may be taken to show one-tenth of one per cent., if the column of fat obtained fills onlj' one or two divisions of the scale. The double-necked bottle is very convenient for the testing of separator skim milk, thin butter milk and whey. The milk, aeid, and water are added to the bottl& ^' FarriDgtoD, and constructed liy Mr. J. .1. Nussljaumer, of Illinois. Fig. 30. The double-necked skira milk bot- tle, tsnmetimes called the Ohls- son or B. A W . bottle). Babcoch Test for Other Milk Products. 77 through the large side-tube; the mixing of milk and acid, anil the addition of hot water, must be done with great care so that none of the contents are forced into the fine measur- ing tube and lost. When the fat is in the lower end of the measuring tube, it can be forced up into the scale b}- press- ing tightly with the finger on the top of the side tube. This test bottle is more fragile and expensive than the ordinary Babcock bottles, and unless carefully handled, will not prove a good investment; the bottle has recently- been made of heavier glass and this form is to be highlj' recommended. Sy. The doul)le-si::ed shim milk Lottie is of no particular value. It is difficult to ol)tain a thorough mixture of the milk and the acid in these bottles, and the tests invariablj' come too low, more so than in case of the regular Baljcock bottles, or the double-necked skim milk bottles. *)0. The testing of hutter milk or wliey by the Babcock test oflers no special difflculties, and what has been said in regard to tests of separator skim milk is equally true in case of these b3'-products. Whej' contains only a small quantit}' of solids not fat (less than 7 per cent), and the mixing with acid, and the solution of the whe^' solids therein, is therefore readily accomplished; the acid solution is of a light reddish color, turning black but verj' slowlj'. 01. Cheese. Cheese can be easily tested by the Bab- cock test if a small scale (fig. 27-8) is at hand for weighing the sample; the results obtained will furnish accurate information as to the amount of fat in the cheese, provided good judgment and exactness is used in sampling and weighing. The following method of sampling the cheese is recommended: * ■ U. S. Dept. of Agriculture, ( hemieul Division, bull. No. 46, p. 37. 78 Testing Milk and Its Products. ■' Where the cheese can be cut, a narrow wedge reaching from the edge to the center of the cheese will more nearly represent the average composition of the cheese than any otlier sample. This maj- be cut quite fine, with care to avoid evaporation of water, and the portion for analysis taken from the mixed mass. When the sample is taken with a cheese trier, a plug taken pjerpendicular to the sur- face, one-third of the distance from the edge to the center of the cheese, should more nearly represent the average composition than anj' other. The plug should either reach entirely through or only half through the cheese. " For inspection purposes the rind may be rejected, but for investigations, where the absolute Cjuantity of fat in the cheese is required, the rind should be included in the sam- ple. It is well, when admissible, to take two or three plugs on different sides of the cheese and after splitting them lengthwise with a sharp knife, take portions of each for the test." 92. When a satisfactory sample of the cheese has been obtained, about 5 grams are weighed into a milk test bottle, or a larger quantit)- may be used with a cream test bottle. The test bottle is first weighed empty, and again after the pieces of cheese have been added. About 15 cc. of hot water is added to the cheese in the test bottle, and this is shaken occasionally until the cheese softens and forms a creamy emulsion with the water. A few drops of strong ammonia will aid in this mixing and disintegration, the pro- cess being hastened by placing the test bottles in hot water. When all lumps of cheese have disappeared in the liquid, the test bottles are cooled to about 70° F., acid is added, and the test completed in the ordinarj' manner. Bahcock Test for Other Milk Products. 79 The percent, of fat in the cheese is oljtained by miiltiply- ing the reading of the fat column Ijy IS and dividing the product by the weight of cheese added to the test bottle. The weighing of the cheese and the reading of the fat must be done with gi'eat care, since an}- error introduced is more than trcl)led in calculating the per cent, of fat in the cheese. Zi 0;j. Condensed milk. The per cent, of fat in con- densed milk can l)e obtained bj' "weighing about s grams into a test bottle and proceeding in exactly the same way as given under testing of cheese. It is not nccessarj- to add ammonia or to warm the condensed milk in the test bottles, since the solution of this in water is readily effected with- out any outside agenc}'. Enough water should be added to make the total volume of liquid in the bottles about 17.6 cc. If a scale is not available for weighing the sample, fairly accurate results maj' be obtained by diluting the condensed milk with water (1:1), and completing the test in the ordi- narj' manner. When this is done, the results must be cor- rected for the dilution which the sample received. So Teslino- Milk and lis Products CHAPraR VI. THE LACTOMETER AND ITS APPLICATION. 94. The Quevenne lactometer. This instrument, (see fig. 31), consists of a hollow glass cylinder weighted down bj' means of mercury or iine shot so as to float in milk in an upright position, and provided with a narrow stem at its upper end, inside of which is found a graduated paper scale. In the better forms, like the Quevenne lactometer shown in the figure, a thermometer is melted into the cj'linder, with its bulb at the lower end of the lactometer, and its stem rising above the lactometer scale. The lactometer is used for the determination of the spe- cific gravity of milk. The term specific gravity^ means the weight of a certain volume of a solid or a liquid substance compared with the weight of the same volume of water at 4P C (39.2^ Fahr.); for gases the standard of comparison is air or hj'drogen. If the milli which a can will hold, weighs exactly 103.2 lbs., this can will hold a smaller weight of water, saj', 100 lbs., as milk is heavier than water; the spe- cific gravity of this milk will then be 11^ = 1.032. 1)5. The lactometer enables us to determine rapidl}' the relative weight of milk and water. Its application rests on ■well-known laws of phj'sics: When a bodj- floats in a liquid, the weight of the amount of liijuid which it replaces, is equal to the weight of the bodj'. It will sink further into a light liquid than inio a heavy one, because a larger vol- Tlic Lactometer and /Is Amplication. Si Fig. :>1. (^ueveuDe lactometer ftouthig io milk in a tin cylinder (100). 6 ume of the former will be required to equal the weight of the bod}". A lac- tocQeter will therefore sink deeper into milk of a low specific gravitj' than into milk of a high specific gravit}-. The scale of the Quevenne lactome- ter is marked at 15 and 40, and divided into 25 equal parts, with figures at each five divisions of the scale. The single divisions are called degrees. The 15 de- gree mark is placed at the point to which the lactometer will sink when lowered into a liquid of a specific gravity- of 1.01 5, and the 40 degree mark, at the point to which it will sink when placed in a liquid of a specific gravity of 1.040. The specific gravit}' therefore is changed to lactometer degrees I)}- multiph-ing I13' 1000 and substractiug lOiiO from the product. Example: Given, specific gravity 1.034-5; corresponding lactometer degree, 1.0345 X 1000 — 1000 = 34.5. {)(). Influence of temperature. Like most li(iuids, milk will expand on being warmed, and the same volume will therefoic weigh less when warmed than before; that is, its specific gravitj' will be decreased. It follows then that a lactometer is onlj- correct for the tem- perature at which it is standardized. If 82 Testing ]\lilk and Its Products. a lactometer sinks to the 32 mark in a sample of milk of a temperature of liO'^ F., it will sink below this mark if the temperature of the milk is 50'' F., and will not sink so far down as :>2, if the temperature is 70" F. Lactometers in the market at present are generallj- standardized at CO'" F., and to show the correct specific gravity, the milk to be tested should first be warmed (or cooled, as the case may be) to exactly 00'" F. As this is a somewhat slow process, tables have been constructed for correcting the results tor errors due to differences in temperature (see Appendix). 97. As the fat content of a sample of milk has a marked influence on its specific gravity at different temperatures, the coeflBcient of expansion of fat differing greatly from that of the milk serum, the table cannot give absolutelj- accurate corrections for all kinds of milk, whether rich or poor. But the errors introduced by the use of one table for any kind of whole milk within a comparatively small range of tem- perature, like ten degrees a))ove or below 60°, ai-e too small to have anj- importance outside of exact scientific work, and in such, the specific gravity is alwaj's determined by means of a picnometer, or a specific gravit}- bottle, at the tempera- ture at which the calibration has been made. In taking the specific gravity of a sample of milk by means of a lacto- meter, the milk is always warmed or cooled so that its tem- perature does not var}' ten degrees either way from Cm- F. 98. The temperature correction table for whole milk, given in the Ap)pendi.i: shows that if, e. g., the specific grav- itj' of a sample of milk taken at 08° F. was found to be 1.034, its specific gravity would be 1.0352 if the milk was cooled down to GO ". If the specific gravity given was found at a temperature of 50°, the correct specific gravity of the TIic LactoDictcr (tiid Its Aj^flication. S^^ In practical work in factories or at tiie farm, sulilciently accurate temperature corrections ma}' generally be made liy adding .1 to the lactometer reading for each degree above (KF F., and l.i}- subtracting .1 for each degree below lii.i : o. g.. if the reading at <\\' is 20.5. it will be about 20.5 - .4 = 2'.\0at CO' F.; and 34.0 at 52' F. will be about :U.o — .S = .'Jo. 2 at GO" F. By reference to the talile in the Ajipendix. we find it to be 33.0 in either case. The Scale of the thermometer in the lactometer should be placed ahove the lactometer scale so that the temperature maj' be read without taking the lactometer out of the milk; this will give more correct results, will facilitate the reading and save time. 9!>. N. Y. Board of Health lactometer. In the East, and among cit}- milk inspectors generally, the so-called New York I>oard of Health lactometer is often used. This does not give the specific gravity' of the milk directly, as is the case with the Quevenne lactometer, but the scale is divided into 120 efjual parts, known as Board of Health degrees, the mark KiO being placed at the point to which the lactometer sinks when lowered into milk of a specific gravitj* of 1.02'.> (at 00" F.); this is considered the lowest limit for specific grav- ity of normal cow's milk. The zero-mark on the scale shows the point to which the lactometer will sink in water; the distance between these two marks is divided into 100 equal parts, and the scale is continued below the 100 mark to 12(1. As 100" on the Board of Health lactometer corresponds to 29" on the Quevenne lactometer, the zero mark showing in either case a specific gravity of 1, the degrees on the former lactometer ma\' easilj- be changed into Quevenne lactometer degrees by multiplying by .29. To further aid in this 84 Tcslinif Milk and Its Prochicls. transposition, a taljle is given in the Appendix, showing the readings of the two scales, l)etween (!(!"' and 120' on the ]?oard of Health lactometer. 1(H>. Reading the lactometer. For determining the specific gravity of milk in factories or private dairies, tin cylinders, ] .', inches in diameter, and Id inches high, with a base about 4 inches in diameter, are recommended, (see fig. 31); another form of specific gravitj' cylinders, in use in chemical laboratories, is shown in fig. 32. The cylinder is filled with milk of a temperature ranging between 5(1 and 70 ' F. to within an inch of the top, and the lactometer is slowly lowered therein until it floats; it is left in the milk for about half a minute before the lactometer- and the thermometer read- ings are taken, both to allow the cscat)e of air which has lieen mixed with the milk in pouring it pre- paralorj' to the specific gravity' determination, and to allow the thermometer to adjust itself to the temperature of the milk. The lactometer should not fie left in the milk much more than a minute ' before the reading is taken, as cream will verj- soon vw.. 32. ]^Q,y\Yi iQ j-ise on the milk, and the reading, if taken spec i lie Kravitv later, will be too high, as the Ijulb of the lactometer (jiiuci.i. ^111 ^^^^ jVjating in partially skimmed milk. In read- ing the lactometer degree, the mark on the scale plainly- A'isi- blc through the upper portion of the surface meniscus of the milk should l)e noted. Owing to surface tension the milk in immediate contact with the lactometer stem will rise above the h;vel of the surface in the cylinder, and this must be takcii into consideration in reading the degrees. There is no need of reading (doser than one-half of a lactometer degree in the practical work of a factory or dairj-. The Lactometer and Its Apph'catioji. S5 101. Time of taking lactometer readings. The specific gravitj' of milk should not be determined until sis to twelve hours after the milk has been drawn from the udder, as too low results are otherwise obtained.* The cause of this phenomenon is not definitel}' understood; it may lay in the escape of the gases in the milk, or in changes in the mechanical condition of the nitrogenous components of the milk, (most likely of the casein), occurring on stand- ing. The results obtained after twelve hours will as a rule come about one degree higher than when the milk is cooled down directly after milking, and its specific gravit}- then determined. Calculation of Milk Solids. 102. A numl.)erof chemists have prepared formulas for the calculation of milk solids when the fat content and the specific gravitj' (lactometer reading) of the milk are known. By careful work with milk tester and lactometer it is possible by means of these formulas to determine the com- position of samples of milk with considerable accuracy out- side of, as well as in, chemical laboratories. As the com- plete formulas given hy various chemists (Behrend and 3Iorgen, Clauscitzer and Mayer, Fleischmann, Hehner and Richmond, Richmond, Babcock,)t are ver)' involved, and recxuire rather lengthy calculations, tables facilitating the figuring have been prepared. The formulas in use at the present time, in this couutr}' or abroad, are those proposed by Fleischmann, Hehner and Richmond, and Babcock. Babcock's formula forms the foundation of the tallies for solids not fat given in the Appendix, as it is generally taught in American dairy schools. * Bulletin No. 4.3, Chem. Div., r. S. Dept. of Agriculture, p. 191. t Agricultural Science, vol. Ill, p. 1.39. 86 Testin}^ Milk and Its Products. B3' the use of these tables the percent of solids not fat corresponding to lactometer readings from 2(] to 30, and to lat contents from 2 to 6 percent may be found. The for- mula, as amended in 1895, is as follows,* S being specific gravit}', and / the percent of fat in the milk. Solids not fat = ( '^^^ ^ ~ '^^ l\ ( 100 — f ) 2.5 VlOO — 1.0753 Sf / 103. The derivation of this formula is as explained in the following: Milk is considered a mechanical mixture of butter fat and milk serum, the latter being an aqueous solution (or in part semi- sf)lution I of all the solid components of the milk, except the fat. 11/ therefore designate the percent, of fat in a sample of milk, then 100 — f = per cent, serum in the milk. Let furthermore the specific gravit}' of the serum be called x, and the increase in specific gravity of the milk caused by one per ceot. of serum solids = a. The difference between the specific jjravity of milk serum and that of water is nearly directly pro- portional to the amount of serum solids present, and the per cent, of serum solids will therefore be obtained if this difference be divided by the constant factor a, representing the increase in specific gravitj' caused by one per cent, serum solids. If this per cent, be multiplied by the per cent, of serum in the milk, and the product divided by 100, the result will give the per cent, solids not fat in the milk, viz. : I'cr cent, solids not fat^ X • . • . (11 a 100 To find the value of x in terms of S, the specific gravity, and /, the fat of the milk, it is necessary to determine the value of a from a large number of analyses. The volume of a substance in cc. is ecjual to its weight in grams divided by its specific gravity ; therefore Volume in cc. of 100 grams of milk = — ^ ' Wisconsin ex|icritiient station, twelfth report, p. 120. The Lactojuctcr and Its Af plication. 87 Volume in cc. of serum in 100 grams of milk = X The specific gravity of pure butter fat being .93, therefore Volume in cc. of fat in 100 grams of milk ^ - - = 1.0753 f .03 Since milk is made up of fat and serum, we have 1^0 = 1211^ + 1.0753 f S X By solving this equation we find that x=^J211- ^--^'' .... (II) i(.»o — 1.075;! Sf To obtain the value a, the last equation is solved for a large number of analyses of different kinds of milk. 1 is subtracted from the average value obtained for x I equation II ) and the difi'er- ence is divided b3' the per cent, of scrum solids. Such calculations have shown that the value of a with normal cow's milk will come very near .004; substituting therefore this value and that of x (ecjuation II) in eciuation I, and reducing, vve have lOi. The tables made up from this formula, giving the percentages of solids not fat corresponding to certain per- cents of fat and lactometer readings, are given in the Ap- pendif. A careful examination of the same discloses the fact that the percent of solids not fat increases uniformly at the rate of .25 percent for each lactometer degree, and .02 percent for each percent of fat. This relation is ex- pressed b}' the following simple formulas: Solids not fat = J L X .2 f Total solids = I Lx 1,2 f, L being the lactometer reading at G0° F. (specific gravity >: 1000 — loOO), and /the percent of fat in the milk. Rule : a, To find the percent of solids not fat in milk, add two-tenths of the percent of fat to one fourth of the lactometer reading, and Solids not fat= I 1-— -^ ^* l\ (100 — f ) 2.5 100 — 1.0753 Sf / SS Tcsling Milk and Its Products. b, To find percent of total solids In milk, add one and two- tenths times the percent of fat to one-fourth of tJie lactometer reading. These formulas and rules are easily remembered, and can be quickly applied without the use of tables. The results obtained by using them do not differ more than .04 percent from those of the complete formula for milks containing up to (i percent of fat, and may be safely relied upon in prac- tical work. Adulteration of Milk. 105. The problem of determining whether or not a sam- ple of milk is adulterated, becomes an important one in the work of milk inspectors, and dairj' and food chemists. Managers of creameries and cheese factories are also some- times interested in ascertaining possible adulterations in case of some patron's milk, although at present, since the general introduction of the Babcock test in factories, and the payment for the milk on basis of the amount of butter fat delivered, the temptation to water or skim the milk has been largely removed. In the cit}^ milk trade, especially in our larger cities, watered or skimmed milk is still fre- quently met with, in spite of the vigilance of their milk inspectors or the officers of the citj^ boards of health. When the origin of a suspected sample of milk is known, a second sample should always be taken on the premises by, or in the presence of, the inspector, and the composition of the two samples compared. If the suspected sample is con- siderably lower in fat content, than the second, so-called control-sample, with a normal percent of solids not fat, it is skimmed; if the solids not fat are below normal, it is watered; and if both these percentages are abnormally low, the sam- ple is most likely both watered and skimmed. The Lactouictcr and Jls AffJicaiioii. S9 106. In order to determine whether or not a sample of milk is skimmed, or watered, or both skimmed and watered, the percents of fat and of solids not fat in the sample mast be ascertained, and if a control-sample can be secured, tliese determinations for both samples compared. The proper latitude to be allowed for the natural variation in the com- position of milk ditfers according to the origin of the milk; in case of milk from single cows, the variations in fat con- tent from day to da}- may exceed one percent, although under ordinar}' conditions the percent of fat in most cows' milk will not vary that much. The content of solids not fat is more constant, and rarelj' exceeds one-half of a per- cent from day to day with single cows. Cows in heat or sick cows may give milk differing considerablj' in composi- tion from normal milk."- 107. jMixed herd milk is of comparativeh' uniform com- position on consecutive days, and as most milk offered for sale or delivered to factories is of this kind, the task of the milk inspector is made consideraiih- easier and more certain on this account. Paily variations in herd milk beyond one percent of fat, and one-half percent of solids not fat, are sus- picious and may be taken as fairly conclusive evidence of adulteration. This is especiall}- true in case the control- sample shows a comparatively low content of fat or solids not fat. lOS. Where a control-sample cannot lie taken, the legal standards for fat or solids in milk, in force in the various states, are used as a basis for calculating the extent of adulteration of a sample of milk. A list of legal standards for milk in this country and abroad is given in the Appendix. These standards determine the limits below which the milk ^ Blytli, Foods, their (/omposition and Analysis, London, lS.s2, p. 2415 et seq. go Tcs/fu^- Milk and lis Products. oliered for sale must not fall within the respective states. TjCgallj' it matters not whether a sample of milk offered for sale has been skimmed or watered by the dealer or bj' the cow; in the latter case, the cows producing the milk are of a breed or strain that has been bred persistently lor quantity of milk, without regard to its quality. In most states the legal standards for the fat content of milk is 3 per cent., and for solids not fat, 11 or O.fi per cent. There are, however, cows that normally will produce milk containing only 2.5 to 2.8 per cent, of fat. and less than 8.5 per cent, solids not fat. Such milk cannot therefore be legally sold in most states in the Union, and the farmer oifering such milk for sale, even if he does not know the composition of the milk produced by his cows, is as liable to prosecution as if he had directlj' watered the milk. By mixing the milk of several cows, the chances are that the mixed milk will contain more fat and solids not fat, than called for bj- the legal standard; if such should not be the case, cows producing richer milk must be added to the herd so as to raise the qualitj' of the herd milk up to the legal standard. Kti). Calculation of extent of adulteration.* In the following formulas, the percents found in the control- samples, if such are at hand, are always sulistituted lor the legal standards. a. Skimming. — ]. If a sample of milk has been skimmed, the following ibrmula will give the number of pounds of fat alfstracted from 100 lbs. of milk: Fat abstracted ^ (x) ^ legal standard for fat — f, . (T) f being the percent of lat in the suspected sample. 2. The following formula will give the [jcrcent of fat ab- * Woll, Uuiidbuiik for Kariiuira iind Dairyiueu, New York, 1S1I7, pp. '207-3. The Lactometer and Its Application. 91 straeted, calculated on the total quautit)- of tat, originally loinid in the milk: x=10()— , j-- . — j-n — — .... (II legal standard tor tat b. Watering. — 1. If a sample is watered, the calculations are most conveniently based on the percentage of solids not fat in the milt. Percent, of foreign ('■ extraneous ') water in adulterated S X 100 milk=100— , ; r~^ p-j — — . . . . (Ill) leg. stand, tor solids not tat *S being the percent, of solids not fat in the suspected sample. Example: — A sample of milk contains 7.5 percent, solids not fat; if the legal standard for solids not fat is 9 percent., 100 — = H). / , shows the percent, or extraneous water ni the 9 milk. 2. Watering of milk maj- also bo expressed in per cent, of water added to the original milk, by formula IT: Percent, water added to original milk , , 100 X lea;, stand, for sol. not fat .,-,,-, ^t-x = (x) = 5 _ 100 . . (R) 100 X 9 In the example given above, ^ — ^ — 100 = 12 percent, of 7.5 water was added to the original milk. c, Watering and slcimniing. — If a sample has been both watered and skimmed, the extent of watering is ascertained by means of formula (III) or (lY), and the fat alistracted found according to the following formula: Percent, tat abstracted = , ^ , , 1 J3 . J- les. stand, for sol. not fat ^. ,,rx .(x)=leg. stand, for fat ■ ^^ Xf .(\ ) 92 Testiiw- Alilk (itid Its Products. Example : — A sample of milk contains 2.4 per cent, of fat, and S.l per cent, solids not fat; then bxtraneous water in milk = 100 — 9 X 2.4. S.l 10 per cent. Fat abstracted = 3 -^ .'.'>?> per cent. 100 lbs. of the milk contained 10 lbs. of extraneous water and ..33 lbs. of fat had been skimmed from it." 110. other methods of adulteration. ^lilk which has l)een watered, or skimmed, or both, is sometimes further adulterated l)y unscrupulous milk peddlers through the addition of a small quantit}' of cheese color; this will mis thoroughly with the milk, and, if added jadiciouslj', will im- part a rich cream color to it. The presence of foreign col- oring matter in milk is easilj- shown bj' shaking ] cc. of the milk with an equal quantity of ether; on standing, a clear ether solution will rise to the surface; the solution will be yellow colored, if artificial coloring matter has been added to the milk, the intensity of the color indicating the quantity added; natural fresh milk will give a colorless ether solution. "The following blank will Ije found convenient for work in the laboratory or testing room : REPORT BY. DAIRY SCHOOL. DATE. 189. .MILK TEST. No. of sample. Laotunie- ter lead- ing. Tempera lure. ( orrectei] reading at 60° Per cent, of fat. Solids not fat. Adultera- tion, kind and amt. Tlie Lactometer and Its Application. 93 111. The Ibllowiug metliod given b}' Wallace* is claimed to detect one part of coloring matter in 100,000 of milk. About 30 cc. of milk are coagulated with a few drops of acetic acid by the aid of heat. The coloring matter of annatto being insoluble in acids, is precipitated with the casein. The coagulated mass is separated from the whe_v by straining through a coarse cotton cloth, and the excess of liquid pressed out. The casein is placed in a mortar and rubljed with ether. The ether is then poured into a separatory funnel and about 10 cc. of a dilute soda solution (1:100) are added. After thorough shaking, the funnel is set aside to allow the litjuids to separate. The lower layer ■which consists of soda solution in which the annatto is dissolved is drawn off into a ])orcellain dish, and in it are placed two discs of filter-paper, and the liquid is greatly evaporated. If annatto is present, the discs will be dyed orange to buff color. One of the well-washed discs is moistened with a dilute sodium carl5onate solution to fi.K the color; the other is touched with a drop of stan- nous chlorid, and becomes instantly changed in color to a rich pink. "This test may be used in any condition of the milk, and with as small a quantity' as 10 cc." It is not known that other methods of adulterating milk than those mentioned are practiced at the present time. For methods of detection of preservatives in milk, see Chapter X. * N. .J. Dairy I 'oinmissiouer Report, 1896, p. .36. 94 Tcslino- Rlilk and Its Products. CHAPTP:?.. VII, TESTING THE ACIDITY OP MILK AND CREAM. 1 \'l. Cause of acidity in milk. Very soon after milk is drawn I'rom the udder, it will be found to have an acid reaction, when phenolphtalein is used as an indicator. '■' The acidit}' in fresh milk is not due to the presence of free or- ganic acids in the milk, like lactic or citric acid, but to acid phosphates, and possibly also in part to free carbonic acid gas in the milk, and to the acid reaction of casein. Even in case of so-called sweet milk, uearlj' fresh from the cow, a certain amount of acidity, viz: on the average .07 per- cent, is therefore found. When the milk is received at the factory it will rarel}' test less than .10 percent, of acid, calculated as lactic acid; some patrons bring milk day after day that does not test over .15 percent, of acid; others In-ing milk that tests from .20 to .25 percent., and some lots, although very rarely, will test as high as .3 of one per- cent, of acid. It has been found that milk will not usuallj^ smell or taste sour, or "turned," until it contains .3 to .35 percent, of acid. 113. The acidit}' in excess of that found normallj- in milk as drawn from the udder, is due to other causes than those described, viz: the presence of acid milk-components. Bacteriological examinations (jf milk from various sources * Freshly drawn milk sbows an amji/iotfrie rcation lo litnni.s. i. c, it colors blue litmus jtaper red, and red litmus paper blue. Testing- the Aa'ditv of j\JiIh and Cream. 95 and of (lifferent age have shown that there is a direct reha- tion between the bacteria found in normal milli. and its aciditj'; the larger the number of bacteria per unit of milk, the higher the acidity of the milk. The increase in the acidit}' of milk on standing is caused b}' the breaking-down of milk sugar into lactic acid through the inlluence of acid- forming bacteria. Since the bacteria get into the milk through lack of cleanliness during the milking, and careless handling of the milk after the milking, this being kept under conditions that favor the multiplication of the liac- teria contained therein, it follows that an acidity test of fresh milk gives a verj' accurate measure of the care bestowed in handling the milk; such a test will show which patrons take good care of their milk and those who do not wash their cans clean, or their hands and the udders of the cows before milking, and have dirt}' waj's generall}' in milking and caring for the milk. The acidity test will always be high in case of milk kept for more than a daj- (Alonday milk) or delivered after a warm sultry daj- or night. The bacteria have had a chance to multiply enormousl}' in such milk, even if it be kept cooled down to 40"-5()- F., and as a result considerable quantities of lactic acid have been formed. The determination of the acidity of fresh milk is explained in detail below (128). 114. Methods of testing acidity. ^lethods of meas- uring the acidity or alkalinity of liquids by means of certain chemicals giving characteristic color reactions in the pres- ence of acid or alkaline solutions (so-called volumetric meth- ods of analysis) have been in use for many years in chemical laboratories. The}' were applied to milk as earl}- as 1S72 by Soxhlet,* and the method worked out by Soxhlet and f Jour. f. prakt. Cbemie, 1872, p. 6, 19. g6 Testing Milk and lis Products. Henkel has since been in general use by European chemists. They measured out 50 cc. of milk to which was added 2 cc. of a 2 percent alcoholic solution of phenolphtalein, and this was titrated with a one-fourth normal soda solution* (see below). In this country, Dr. A. G. Manns, t in 1890, pub- lished the results of work done in the line of testing the acidity of milk and cream, and the method ol procedure and apparatus proposed by him has become known under the name of Manns test, and has been advertised as such b}^ dealers in dairy products, 115. Manns' test. The acid in milk or cream is measured bj' using an alkali solution of a strength, together with an indicator, which shows bj' a change of color in the milk when all its acid has been neutralized, Anj' of the alkalies, soda, potash, ammonia, lime or barium can be used for making the standard solution, but it requires the skill and apparatus of a chemist to prepare it of the proper strength, A one-tenth normal solution + of caustic soda is the alkali solution used most frequentlj' in determining the acidity of milk, and is the solution labeled Meutralizer of Manns' test. * Fleischmann, Lehrb, d. Milchwirtschaft, p. 23. f Illinois exiieriment statioD, bulletin No. 9, X A normal solution of a chemical is known by the chemist as a solution contain- ing as many grams of the chemical in a liter (1000 cc.) as the figure representing its molecular weight. Caustic soda is made up of an atom each of sodium (Na), oxygen (0), and hydrogen (H); its molecular weight is therefore ■2:) -I- 16 -h 1 = 40 Na O U A normal soda solution then is made by dissolving 40 grams of soda in water, making up the volume to 1000 cc.; a one-tenth nortual solution will contain one- teritb of this amount of soda, or -1 gr, dissolved in one liter. One cubic centimeter of the latter solution will contain .004 grams of soda, and will neutralize .009 grams of lactic acid. The formula for lactic acid is C;) H,.,03 (see p. 10) and its molecu- lar weight therefore 'i / 12 -I- 6 < 1 + 3 >: 10 =- 90. A one-tenth normal solution therefore contains 9 grams per liter, and .009 grams per cubic centimeter. Testing- the AciditY of Milk and Cream. g"] The indicator used for this purpose is phenolpldalein^ a. 3'ellowish hght powder; its compounds with alkalies are red, in weak alkaline solutions, pink colored; while its acid compounds are colorless. The ijhenolphtalein solution used is prepared by dissolving 10 grams in 300 cc. of IJO percent, alcohol (Mohr). IIG. In testing the acidity of either milk or cream it is necessary to measure out with exactness the quantity of lii{uid to be tested; ]\Ianns recommended using a 50 cc. pipette. This amount of milk or cream is measured into a clean tin, porcellain or glass cup, a few drops of the phenol- phtalein solution are added, and the "Neutralizer" (or alkali solution) is cautiousl}* dropped in from a Ijurette, the point at which the solution stands before it is drawn out being noted. By constant stirring during this operation it will be noticed that the pink color formed by the addition of even a drop of alkali solution will at first entirely disappear, but as more and more of the acid in the sample becomes neu- tralized, the color will disappear more slowly-, until finall}- a point is reached when the pink color remains permanent for a time. No more alkali should l)e added after the first ap- pearance of a permanent and uniform pink color in the sample. This color will fade and gradually disappear again on standing, owing to the effect of the carbonic acid of the air, to which acid, phenolphlalein is very sensitive. The amount of the alkali solution used for the test is then ob- tained from the reading on the scale of the burette, and this shows the degree of acidity in the sample. The per cent, of acid in the sample is calculated by multiplj-ing by .009 the numtier of cc. of alkali solution used, and dividing the product b}^ the number of cc. of the sample tested, the quotient being multiplied b)' 100. Tesliiio Milk and lis Prodiicls. Percent acidity = - c. c. alkali x .000 100 c. c. sample tested ' If 5(1 cc. of cream required 32 cc. of alkali solution to produce a permanent pink color, the percent of acid in the ■i2 X .let being always added for each ounce of water: four tablets in a four ounce bottle; six, in a six Testing tlic Aciditx of Milk and Cream. 107 ounce Ijottle, etc., the amount of tablet solution prepared depending on the number of tests to be made at one time. The bottle is filled up to its neck with clean soft water, and the solution prepared in the manner previously given (124). The manner of operating tlie test is as follows: 5 OuiiceT3oUle. "Measure Fig. .36. Apparatus used for rapid estimation of tl:ie acidity of apparently ^weet luillc or cream. 12!>. Operating the test. As each lot of milk is brought to the creamery in the morning and poured into the weigh car, it is weighed, and the cartridge-shell dipper filled with the milk; this is then poured into the white cup. The same or another Xo. 10 shell is now filled twice with the tablet solution, and emptied into the milk in the cup. Instead of dipping twice with one measure or a No. 10 shell, a tin measure can be made holding as much as two No. 10 shells. The liciuids are then mixed in the cup by giving this a (piick rotar}' motion. The color of the mixture is now io8 Testing Milk and lis Products. noticed. If the milk remains while it contains more than two-tenths of one percent of acid, and should not Ije used for pasteurization. If it is colored after having been tlior- oughly mixed with two measures of tablet solution, it con- tains less than this amount of acid and may be safely used, as far as acidity goes, for pasteurization or anj' other pur- pose for which it is neces8ar3- to have thoroughly sweet milk. The shade of color o)>tained will vary with different lots of milk; the sweetest milk will be most highl}' colored, but a milk retaining even a faint pink color with two measures of tablet solution to one measure of milk contains less than .2 percent acid. Ky proceeding in the manner described, the man at the factory weigh can is able to test the acidity of the milk de- livered nearly as quickly as he can weigh the milk; and ac- cording to the results of the test he will send the milk to the general deliver}' vat, or to the pasteurization vat, as the weighing can maj' be provided with two conductor spouts. 130. iiirx of measure, necessary. It is not necessary to use a No. 10 shell for a measure in working the preceding method; one of an}' convenient size that can be filled ac- curately and quickly, will answer the purpose equally well, if a measure of the same size is used for lioth the sample and the tal:)let solution. When this is done, each measure- ful of tablet solution made up as directed, will represent one-tenth percent of acid in the sample tested. 1 :} 1 . b, Cream. Cream can be tested in the waj* already described for testing the acidity of fresh milk, liy adding to one measureful of cream in the cup, as manj- measures of tablet solution as arc necessary to change the color (jf the cream when the two liquids are thoroughly mixed. If one Testing the Aeidity of Milk and Cream. 109 measure of tablet solution colors one measure of cream, this contains less than .1 percent acid; if five measures of tablet soluLion are required, the cream contains about .5 percent acid, etc. By proceeding in the manner described, the ope- rator can estimate the acidit}' to within ,05 per cent, of acid, if half measures of tablet solution used are observed. The results thus obtained are sufficientl}' delicate for all practical purposes. 132, Detecting' j)reservaline in iiiilk. The tablet solution furnishes a simple method of detecting preservalinc in milk. The application of the alkaline tablets for this purpose was first dis- cussed in bulletin No. 52 of Wiseonsin experiment station. The aciditj- of the milk is increased b}- the addition of preservaline, but neither the odor nor taste of tlie inilk is affected thereby. By ad- diiiij to sweet milk the amount of preservaline which the manu- facturers claim will keep it sweet for 36 hours, its acidity nia^' be increased to .3.") per cent., in a sample of milk which before adding the preservaline tested perhaps only .15 per cent. acid. As before stated, unadulterated milk will usually smell or taste sour, or "turned ", when it contains .30-. ,35 per cent, acid ; milk testing as high as this limit, which neither smells nor tastes sour in any wa3', is therefore in all probabilitv adulterated with pre- servaline or some other preparation containing boracic acid, or a similar compovmd. X'i?}. "Alkaline Tabs." These are not alkaline tab- lets, but a substitute which was put on the market )iy a New York firm. The outfit furnished consisted of four packages of paper discs made of filter paper, each of about the size of an old-st^'le copper cent; two packages of square paper; one glass of about 10 cc. capacitj-, and one small * Preservaline is the trade name of an antiseptic extensively advertized for preserving mill^ and creaiD. It consists essentially of boracic acid and boras, the use of which in milk and other dairy prodn.ts offered for sale, is prohibited by law in many states. no Tcstino' Milk and lis Products. i> glass bottle. The directions stated that each paper disc represented .1 per cent, acidity when added to the small glassful of milk or cream, with two of the square papers, the whole to be well shaken in the long glass bottle. The acidity of the sample of milk or cream was claimed to be measured by counting the number of round papers required to produce a pink color in the sample tested. An investigation of the reliability of these " Tabs " soon disclosed the fact that thej' were entirel}' inaccurate, and that no dependence could therefore be put on the results oljtained bj' their use. A report of the comparative work done in testing the acidity of milk or cream by a one-tenth normal alkali solution and these " Alkaline Tabs " was pub- lished in the dair}^ press in 1S95, to which reference is here made as to the details sf the results obtained.* '■ Hoard's Dairyman, Sejit. G, 1395. Testing the Purity of Milli. Ill Fig. 37. Students operating the Wisconsin curd test. CHAPTER VIII. TESTING THE PURITY OF MILK. 134. The Wisconsin curd test. Cheese makers are often troubled with so-called floating or gassy curds which produce cheese defective in flavor and texture. These faults are usuallj' caused by some particular lot of milk containing H2 Tcstino ]\/ilk and lis Prochicls. impnrilies that cannot be detected by ordinaiy means of in- spection. The Wisconsin curd test is used to detect the source of these defects and thus enable the clieese malier to exchide the milk from the particular farm or cow to which the trouble is traced. This test is similar in princi- ple to tests that have for manj' j'ears past been in use in cheese-making districts in Europe, notablj- in Switzerland,* but was worked out independently at the Wisconsin I)air3' School in 1895 and has become generallj' known as the Wis- consin curd test from the description of it in the report of Wis. experiment station for ]8ll5.t The method of operat- ing this test is as follows (see fig. 37). 135. Method of malcing the test. Pint glass jars, thor- oughly cleaned and sterilized with live steam, are provided; they are plainlj' numbered or tagged, one jar being pro- vided for each lot of milk to be tested. The jars are filled about two-thirds full with the milk from the various sources (it is not necessarj' to take any exact quantitj'); thev are then placed in a tank or vat containing water, which is heated until the milk in the jars has a temperature of 98° F. The thermometer used must not fie transferred from one sample to another, unless special precautions are taken, for fear of contaminating the pure lots of milk hy impure ones. When the milk has reached a temperature ol 98-, add to each sample, 10 drops of rennet extract, and mix l)y giving the jar a rotary motion. The milk is thus curdled, and the curd allowed to stand for about twenty minutes until it is firm. It is then cut One with a case knife, and after settling, the whej' is poured off The best tests are made when the separation of the whey is most complete. Bj- allowing the « Iferz, Uutors. d. Kuliniilcli, Borlin, 1883, p. 87. t Twelfth report, p. MS. Testing the Purity of Millc. 113 samples to stand for a short time, more whey can lie poured oft' and the curd tbereb}' rendered firmer. The water around the jars is kept at a temperature of 93^, tlic vat is covered, and the curds allowed to ferment in the sample jars for six to twelve hours. Paring this time the impurities in any particular sample will cause gases to be developed in the curds so that liy examining the same carcfuUv, l)v smelling of them, and cutting them with a sharp knife, those having a liad flavor or a spong}' or in anv way abnormal texture maj' be easilj' detected, and the lot of milk from which it was made, therebj- picked out. 1B6. ]>y proceeding in the same way with the milk from tlie different cows in a herd, the mixed milk of which pro- duced abnormal curds, the source of contamination in the herd ma}' ))e located. Very often the trouljle will lie found to come from the cows' drinking foul stagnant water ov from fermenting matter in the stable. In the former case the pond or marsh must be fenced off, or the cows kept away from it in other ways; in the latter, a tliorough cleaning and disinfection of the premises are recpiired. If the milk of a single cow is the source of contamination, it must be kept by itself, until the milk is again normal; under such conditions the milk from the health}- cows may of course safely be sent to the factory. 137. The fermentation test. The (lerber fermenta- tion test (see fig. oS) furnishes a convenient method for discovering the cause of abnormal fermentations which show themselves in tainted, pin-holey, gassy, or floating curds, aud is also useful in examining the purity of different lots of milk. The test consists of a tin tank which can be heated 14 Tcsliu'i- Milk and Its Products. bj' moans of a small lamp, and into which a rack fits, hold- ing a certain number of cylindrical glass tubes; these are all numbered and provided with a mark and a tin cover. In making the test, the tubes are filled to the mark with milk, the number of each tube being recorded in a note book, opposite the name of the particular patron whose milk was placed therein. The tubes in the rack are put in the tank, which is two-thirds full of water; the tempei'ature of the 3S. The Gerbcr feriumlal i water is kept at 104-100'"^ F. for six hours, when the rack is taken out, the tubes gently shaken, and the a))pearance of the milk, its odor, taste, etc., carefully noted in each case. The tubes are then again heated in the tank at the same temperature as liefore, for another six hours, when oljserva- tions arc once more taken of the appearance of the milk in each tube. The tainted milk may then easily be discovered by the abnormal coagulation of the sample. Testing the Purity of Milk. 115 According to Gcrlier,* good and proijcrh- handled milk sliould not coagulate in less than twelve hours, when kept nnder the conditions described, nor show an3'thing abnor- mal when coagulated. [Milk from sick cows and from cows in heat, or with diseased udders will always coagulate in less than twelve hours. If the milk does not curdle inside of a da}' or two, it shoukl be tested for preservatives (210). ^- I>ie praktisclie itilch-Pruf lung; A\"on, HauiJijook 1'. Farmers auJ Dairymen,, pp. 2.5.';~.5. Tcsliiit!- i\/ilk and Jls. P) odurls. CHAPTER TX. TESTING MILK ON THE FARM. i;»9. Variations in milk of single cows. The varia- tions in tlie tests of milk of sintfle cows from mill- Mill on t/ic Farm. up Average results obtained in loeigliing ami testing a cow's milk elaily during one period of laetation. IMONTII. December January,., February. JNIarcli April May June July August Daily luilk vi'eld. III47 11154 1117:1 110.1 IbSIl 1130 It',. {I 16, 1 14. :i 1:1s I4.,T ri. 1 0, 3 6,4 17.7 17.7 16.0 16.5 17.2 14.0 1-2.2 9.3 10. fl H.o 13..: 12.5 11.5 10. II. 2 6.0 Tfsts of one day's milk. ■i.^ 4.9 3 3. 7 4.6 2. 7 3.(; 5. >■ 3.2 3. H 4.7 3.4 4.0 5.S 3.0 3. S 4.6 3.4 3.9 4.6 3. 2 1.2 6 2 2.S 4.7 7. 9 2. 9 Yield of fat per day. .54 .55 .55 11"'. The average test of this eow's milk for her whole period of lactation was 3.8 per cent, of fat (i. e. the total quantit}- of fat produced ^ total milk j'ield, x 1"") ; the milk of the cow twice during this time tested as higli as fi.S per cent., and once as low as 2.7 per cent,, while tests of 3.0 and 4.6 per cent, were obtained a numlier of times. The aver- age weight of milk produced per da}- bj' the cow was 14 lbs.; this multiplied by her average test, 3.8, shows that she pro- duced on the average .53 lbs., or about one-half a pound of liutter fat per daj' during her lactation period. If, how- ever, her flutter-producing capacitj- had been judged by the test of her milk for one daj- onlj', this test might have been made either on the day when her milk tested 5.8 per cent., or when it was as low as 2.7 per cent, lioth of these tests were made in midwinter when the cow gave about 1(1 lbs. of milk a day. IMultiplying this quantity by 5.8 gives .113 lbs. of fat, and by 2.7 gives .43 lbs. of fat. Either result might show the butter fat produced by the cow on certain daj's, but neither gives a correct record of her actual average daily performance for this lactation period. I20 Tcslriig ]]/ilk (did lis Products. A sufficient numlier and variety of tests of tlie milk of many cows liave been made to prove tliat there is no defi- nite regularity in the daily variations in the richness of the milk of single cows. The only change in the quality of milk common to all cows is, as stated, the natural increase in fat content as the cows are drying off, and even in this case the improvement in the quality of the milk sometimes does not occur until the milk yield has dwindled down very materiallj'. 14:{. Causes of variations in fat content. The quality of a cow's milk is as a rule decidedlj- influenced by the fol- lowing conditions; Rough treatment. Exposure to rain or rough weather. Change of feed. Change of milkers. Rapidit}' of milking. Length of interval between milkings. Unusual excitement or sickness. Itt-t. Pisturbances like those enumerated frequently in- crease the richness of the milk for one, and sometimes for several milkings, but a decrease in qualitj' follows during the reaction or the gradual return to normal conditions, and taken as a whole, there is a considerable falling off in the total production of milk and butter fat by the cow, on ac- count of the nervous excitement which she has gone through. Aside from changes due to well-definable causes, like those given aliove, the quality of some cow's milk will often change very considerablj- without any apparent cause. The dairj'man who is in the habit of making tests of the milk of his individual cows at regular intervals, will have abundant material for study in the results obtained, and he will soon Testing iMilk on the Farm. 121 be able to tell i'rom the tests made, if these are continued for several days, whether or not the cotts are in a n(.irmal healthy condition, or have been sultjected to excitement or abuse in an}- way. \\7). Number of tests required during a period of lactation in testing cows. Tlie daily records of the six cows referred to on p. 118 give data for comparing their total production of milk and liutter fat during one period of lac- tation, as found from the daily weights and tests of their milk, with the total amount calculated from weights and tests made at intervals of 7, 10, 15 or 3(1 days. The aver- ages of all results obtained with each of the six cows show that weighing and testing the milk of a cow ever}' seventh day, gave 98 per cent, of the total milk and butter fat, which according to her daily record was the total product. Tests made once in two weeks gave 97. G per cent, of the total milk, and 98.5 per cent, of the total butter fat, and tests made once a month, or onlj' ten times during the period of lactation, gave 96. J: per cent, of the total milk, and 97 per cent, of the total production of butter fat. 146. The record of one of the cows will show how these calculations are made: It was found from the daily weights and tests that cow No. 1, in one lactation period of 3(17 days, gave 5,01:4 lbs. of milk which contained 254 lbs. of Initter fat. Selecting every thirtieth day of her record as testing day, the total production of milk and fat is shown to be as follows: Testing J\lrlk and //s Products. ProduclJon of milk and hatter fat per day. Testing day. Weight of millt. Test of Ijlilk. Yield of liutter fat. Nov. 4 Dec. 4 Jan 3 lbs. 20.5 IS. 7 17.7 20.0 1R.2 19.5 17.7 13.1 12.2 ]jer cent. 4.7 4.G 4.0 4.5 4.7 4.4 4.8 5.5 0.2 7.2 lbs. .06 .86 86 Feb. 2 Mar. 3 .00 .86 ■ April 2 Mav 2 .81 .85 Tune 1 Tulv 1 July 31 .72 .76 Total, 150.7 lbs. 15.07 lbs. 7 SI lbs. Average per day. 4.80 .7s lbs. The average dailj' production of the cow, according to the figures given in the preceding table, was nearlj* 1(» lbs. of milk, containing ."78 lbs. of butter fat. Multiplying these figures b}- !!07, the number of days during which the cow was milked, gives 4003 lbs. of milk and 240 Ujs. of fat. This is 141 lbs. of milk and 14 lbs. of fat less than the total weights of milk and butter fat, as found by the daily weights and tests, or 2.8 and 5.5 percent less, for milk and fat pro- duction, respectivel3'. This is. however, calculated from onl}' ten single weights and tests, while it required over GOf) weighings and IjOO tests of the milk to obtain the exact amount. Similar calculations from the records of the other cows gave fully as close results, showing that quite satisfactory records of the total proiluution of milk and l)utter of a cow ma}' be obtained )iy making correct weighings and tests of her full day's milk once every thirty days. Tcsliiio- 2/ ilk on //ic Farm. i2'3 147. When to test a cow. The Vermont experiment station for sevei-al years made a special study ol' the ques- tion when a cow should be tested in order to give a correct idea of the whole 3-ear's performance, when only one or two tests are to be made during the lactation period. ' The results obtained may be brieH3- summarized as follows: a. As to (quality of mil]; produced. If two tests of each cow's milk are to be made during the same lactation period, it is recommended to take composite sami)les at the inter- vals given below. For sprintj co\vs. ('» weeks atter calving, (i"! ■2—~^-2 mos. after calving summer " 8 " " " (5 —7 tnos. " fall " S-10 " " " 15I2-7 mos. If only one test is to be made, approximately correct re- sults may be obtained b}' testing the milk during the sixth month from calving, in case of spring cows; during the tliird to fifth month in case of summer calving cows, and daring the fifth to seventh month for fall calving cows. In all cases composite samples of the milk for at least four days should lie taken (lt!2). " The test of a single sample, drawn from a single milking or daj', will not of necessity, or indeed, usually, give trustworthy results," This method of obtaining tlie average composition of the milk produced duriug a lactation period is naturally more correct in case of mixed herd milk, than when single cows are tested, b. As to ijuantity of milk produced. The milk may be weighed for four days in the middle of the month, and the entire month's yield obtained with considerable accuracj- (barring sickness and drying off), liy multiplying the sum by ■ Sixth rei'Ort, IS'J'2, ]) lOG: Ninth report, rstl.T, p. 1,6. 1-4 Tcslhi''- Mill; and Its Products. 7, 7,', or 7:,', accoriling to the luimher of ilays in the different monllis. TIic weighing is most readily done hy means of a spring balance, the hand of whicli is set l)ack so tliat the empty i)ail Ijrings it to zero (lig. :i!i). If several pails are to be used, thej' should first Ije made to weigh tlie same Ijy putting a little '^THE DAIRYMENl'S SUPPLY C0> ' PHILA. PA. '^y^M'^jki imwi%. Fic;. :I9. Milk scale. l-'io. 40. Automat]!, milk rcgiHterint^ the tiiilk i for woif,'liing and individual cows. solder on the lighter [jails. IMilk scales which weigh and automatically register the yield ol' milk from twenty cows have recently been placed on the market (see fig. 40); the pail is hung on the hook, and liy pressing the button show- ing the number of the i)articuiar cow, the weight is recorded on the m ilk sheet. Tcslhig Milk on the Fudu. 125 14-8. n. B. Gurler, the well-known Illinois dairyman, suggests"'' a method of determining the total prodaetiou of a cow during one lactation period from the test of her milk for one week only. This i)lan is, however, onl}- recom- mended to those who cannot or do not care to take the neces- sary time to make a more reliable test. The test should be made after the cow has been in milk for about three months. It is necessary that the cow should be producing a normal cjuantity and ciualit}' of milk at the time when the the test is made; if, e. g., a cow is fresh Slarch 1, and tested June 1, she is probalily on good pasture feed, and produces more milk and butter fat than at any previous or subsequent time. She should, therefore, be tested either liefore she is put on pasture or after the luxuriance of the pasture is gone. Mr. Gurler suggests that the milk be weighed for a week, and a composite sample taken of the milkings. At the end of this time it may be found that the cow gave 154 lbs. of milk during the week, and the com- posite sample tested 4.3 percent fat; the cow, therefore, pro- duced 'CiXi lbs. of Initter fat during the week, or on the average, .04 llis. per day. This average yield is now multi- plied by 252, the number of daj's in S.4 months, and a yield of 2:>7 lbs. of butter fat is obtained. This will very nearly represent the total production of fat by the cow during the whole period of lactation. It is assumed in this calculation that the cow gives milk more than 8.4 months, and that what is produced beyond this time will bring the production during the last 2.4 months up to the same average per month as in the first six months. Amtriiiui Dairying, |i. I^. Tcstiii!'- Milk and lis Products. WW. Record of tests. 'W'here tests of the single cows ill a herd are made regularly, a complete record should ))e kept in a cote liook arranged in about the manner shown on the following blank. RECORD OF TP:STS OF COWS. MONTH.-- , IS!) Name of cow. Pounds of iiiilk. No. of test liotllo. Percent. of luitlcr I'al Yir-M (,r Imllcf fill pun rll]^^ l.")(). Sampling milk of single cows. In sampling the milk of single cows, all the milk ol)taincd at the milking must lie thoroughly mixed, b}- pouring it from one vessel to another for a few times, or stirring it thoroughly b}- means of a dipper moved up ami down, as well as horizon- tally in the pail or can in which it is held; the sample for testing purposes is then taken at once. \ correct sample of a cow's milk cannot be obtained by milking directly into a small bottle from one teat, or I.)}' filling the bottle with a little milk from each teat, or hy taking some of the first, midiUe, and last milk drawn from the udder. Such samples cannot possibly represent the ({utility of the milk of one entire milking, since there is almost as much difference hc- tween the first and the last portions of a milking, as between skim milk and cream.* Lack of care in ttiking a fair sam[)le is the cause of many surprising results obtained in testing milk of single cows. 151 . When a cow is to lie tested, she should lie milkeil (lr3' the last milking i)i'cvious to the dti}' when the test is to *Woll, llajidl.ook lor I'luiiicre ;uh1 liairyiorn, p. I'j-I; .XKrieultural Scit'Mco, i'., Testiuq Milk on the Farm. 127 be made. The entire quantitj? of milk obtained at each milking is mixed and sampled separate!}'. On account of the variations in the composition of the milk, a number of tests of successive milkings must be made. As this involves considerable labor, the plan of taking composite samples is preferable; the method of composite sampling and testing is explained in detail under the second subdivision of Chapter X (1C2) ; suflice it here to say that the method followed in case of single cows' or herd milk, is to take about an ounce of the thoroughly mixed milk of each milking; this is placed in a pint or quart fruit jar containing a small quantity of some preservative, preferablj' about one-half a gram of powdered potassium l)i-chi-omate. If a number of composite samples of the milk of single cows are taken, each jar should be labeled with the number or name of the particular cows. Composite test.s are generally taken for four daj's or for a week. If continued for a week, the jars will contain at the end of this time a mixture of the milk of fourteen milk- ings. The composite sample is then carefullj' mix:ed by pouring it gentlj' a few times from fine jar to another, and is tested in the ordinary manner. .The result of this test shows the average quality of the milk produced by the cow during the time the milk was sampled. 152. As the amount as well as the qualit}' of the milk produced b}' single cows vary somewhat from day to da}% and from milking to milking, it is quite important in testing single cows, especiallj? when the test includes only a few days, to take a proportionate part (an aliqnot) of each milk- ing for the composite test sample. This is easilj' done b}^ means of a Scovell sampling tube, the use of which is ex- plained in another place (Iii6). 128 Tcs/iiii' Jlfilk and Its Producls. 153. Size of the testing sample. Four ounces is a suflicient quantity for a sample of milk if it is desired to determine its percent of fat only; if tlie milli is to be tested witli a lactometer, when adulteration is suspected, as much as a pint is needed for a sample. If this sample of' milk is put into a bottle and carried or sent away from the farm to be tested, the liottle should be completelj- filled with milk, to prevent a partial churning of butter in the sample during transportation (25). \'^^r. Variations in herd milk. While considerable variations in the quality of milk of single cows are often met with, a mixture of the milk of several cows, or of a whole herd, is comparative!}- uniform from day to day; the individual differences tend to balance one another so that variations when the}' do occur, are less marked than in case of milk of single cows. There are, however, at times marked variations also in the test of herd milk on successive days; the following figures from the dairy tests conducted at the AVorld's Columliian Exposition in Chicago in 1803 will show the correctness of this statement. The test included twenty- five cows each of the Jersey and the Guernsej- breeds, and twenty-four of the Shorthorn breed. Tests of lierd milk on successive days. Date. July 16, ISD.'i. July 17, IH'.i;!. luly IS, is'.i;i. Inly I'J, IS!).''.. July 20, 1S'J3. Jersey. ■1 ,S pcrccut. ,'",.(1 4.7 4.(1 r>.o f.C) ])crceiit. f.r> 1. 1 t.C. 4.ri Sliorthor ;i.S |RTcrnt. ;^.s ;-<.s a. 7 8.S Tcsling Milk uii t/ic Farm. 129 (.)ii July 17, ISil.';, the mixed milk of the Jersey cows tested two-teuths of oue percent higher than on the preceding day; the Claernsey herd milk tested oue-tenth of oue per- cent lower, while the Shorthorn milk did not change in composition; comijaring the tests on duly l!i and I'll, we find that the Jersey anil Shorthorn milk tested rour-teuths and one-tenth of one |)crcent higher. rcspecti\"cly. on the hitter day than on the former, and the (juernsey milk tested one- tenth of one percent lowi^r, 1."),"). Ranges in variations of herd milk. According to Fleischmaun,* herd milk on single days may \-ary from the average values for the year, as follows, expressed in [ler- cent of the latter; The s|>ecific gravity (expressed in degrees) may go ahove or below the ^-early average by more than 1 percent. The percent of fat content may gf> above or below the yearly average by more than 0(1 percent. The percent of total solids maj' go above or lielow the yearly' average fi)' more than 14 percent. The percent of solids not fat ma}- go al)o\-e or lielow the yearl}' average by more than 10 percent. To illustrate, if the average test of a herd during a whole period of lactation is 4.0 percent, the test on a single day may exceed 4.1) + ,■',,",, X 4,0^ 5.2, or niay go below I'.S per- cent, (viz. 4.0 — 1!", >; 40); if the average specific gra\-ity is l.o:-il (lactometer degrees o1),t the specific gravit}- of the milk on a single da\' ma}' vary between I.Ol.'7'.i and 1.0341 (31 + ,',;;, X 31 = 34.1; 31 — ,',;:, x 3,1 =27.11. • Book of thf Hairy, p. ::2. f Se.^ pa^e 81. 9 130 TcsliiiL^ Milk and lis Prod mis. 15<). Influence of heavy grain feeding on the quality of milk. If cows are not starved or underfed, an incrase in the foedinj;- ration will not materially (-liaiiiie the richness of the milk produced, as has lu^eii shuwn b}- careful feciling experiments, conducted under a great variety of conditions and in many countries. (!ows that are fairly well led will almost invariably give more milk when their rations are in- creased, but the milk will remain of about the same quality after the lirst few days are passed as before this time, pro- vided the cows are in good health and uns. of oil meal. All cows gaiuiMl in milk on this fiH'd ; cow N". .'! thus gave an averagt^ of I lbs. more milk per day in -lauu.-iry lliau December, but llie avei'age test of her milk was :',.7 [lerccnt, or one-tenth of (ine |iercent Testing- Milk on I lie Fari)i. 131 lower than during the preceding month. Tiie heavy grain feeding was continued through February and March, when it reached 12 llis. of timothy hay, 12 lbs. of corn and cob meal, 6 lbs. of wheat bran and 6 lbs. of oil meal per day. The records show that the flow ot milk kept up t(.) 1(1 lbs. per daj' in February, in case of this cow, but fell to 14 lbs. in 3Iarch and April, the average test of the milk lieing, in February, 3.6; in March 3.S, and in April, 4.0 percent. The milk was, therefore, somewhat richer in April than in December, but not more so than is found normally, owing to the progress of the period of lactation. 158. Influence of pasture on the quality of milk. On May 1, the cows were given luxuriant pasture *'eed and no grain; a slight increase in the average amount of milk produced per day followed, witli a reduction in the test, this being 3.8 percent, — the same as in December. During all these changes of feed, there was, therefore, not much change in the richness of the milk, while the How of milk was increased b_y tlie heavy grain feeding for several months, as well as I)}' the change from grain feeding in the barn to pasture feed with no grain.* 159. The increase in the amount of Initter produced by a cow, which has often been observed as a result of a cliange in feed, doubtless as a rule, comes from the fact that more, but not richer milk is produced. The qualit)' of; milk which a cow produces is as natural to her as is the color of her hair, and is not materially changed b}' any special system of normal feeding.! * For further data on thi.s point, see Cornell (N. Y.) e.\|i. stu., bulletins 1.-;, 22, 36 and 49; N. D. exp. sta , bull, lii; Kansas exp. sla., report ISSS; Hoard's 1 'airy- man, 1896, pp. 92i-,5. f On this point almost endless discussions have in recent years taken jtlace in the agricultural press of this and foreign countries, and the subject has been uoder 132 Testing Milk and lis Proditcls. 1()(). Method of improving the quality of milk. The quality of the milk produced liy a herd can generally be im- proved by selection and breeding, i. e., by disposing of the cows giving poor milk, say below '.', percent of fat, and by breeding to pure-bred or high-grade bulls of a strain that is known to produce rich milk. This method cannot work wonders in a day, or even in a year, liut it is the only cer- tain way which we have to improve the quality of the milk produced by our cows. It may be well in this connection to call attention to the fact that the quality of the milk which a cow produces is only one side of the question: the <|aantity is another, and eijually important one. Much dissatisfaction and grumbling aljoiit low tests among patrons of creameries and cheese factories would be stopped if this fact was more generally borne in mind. A cow giving 3 percent milk should not be condemned because her milk does not test 5 percent; she may give twice as much milk per daj' as a 5 percent cow, and will therefore produce considerabl3' more 1)utter fat. deliatH iit Dearly evt^ry gatliering of farmers where feeding problems have I)een cod- sidered. Maoy fanners are lirm in their Vjelief tliat butter fat can be ■' fed into" the inilli of a cow, and would talce exception to the conclusion drawn in the pre- ceding. The results of cireful investigations by our best dai ry authorities point conclusively, however, in the direction stated, and the evidtunje on this point is overwhelmingly against the opinion that the fat content of the milk can be ujate- rially and for any length of time increased by changes in the system of feeding. The moit conclusive evidence in this line is jierhaps the Danish co-operative cow feeding experiments, conducted during the pasi fen years, with over 1^, nun cows in all. The conclusion arrived at by the director of the Copenhagen experiment sta- tion, UNI I ir wliDse Slip ; -vision and direction the experiments have been ( ondncted has belli stati-d over ami over agnin in the piiblisbisl repnrls of the stalion: that till' ili;iiigi-s of firil iiiale In I he il i H.-rrnl bits iil cows incluibd in tbe experiments have liail priiclirally nu i ii (liiinr ■ on tin' r liriiiiral innipusil icni (I be fat content) of thciiiilk piijil iir -il 111 I lirsr rx |ii riinnils griii n lot lis have been fed against roots, against oil ralte. and against win at bran oi sborl>; grain anil oil eaki s have further- more been bit against roots, .iiiil roots have lieen given as an additional feed to the standaril ralimis tiieii, — in all cases with tlie same negative results as far as changes la tbe fat eontents of tin: milk produced are concerned. Tcs/iiit^' Milk oil l/ic Farm. 133 The point wlietbor or not a cow is a persistent milker is also of primar}- importance; a prodiicliou of yoO llis. of butter fat during a whole period of lactation is a rather high daii y standard, but one reached in' many' herds, even as the aver- age for all mature cows in the herd. I)air3'racu should re- member that a high production of butter fat in the course of the whole period of lactation is of more importance than a very high test. f34 Tcsiiiii'- Mill': cind Jls Products. CHAPTER X. COMPOSITE SAMPLES OF MILK. 1()1. Shortly after milk testing Lad been introduced to some extent in creameries and cheese factories, it was sug- gested by Patrick, then of Iowa experiment station,* that a great saving in laljor, without a coincident diminution in the accuracy of the results, could be obtained by mixing the daily samples of milk from one source, and testing this mix- ture, instead of each sample contributing thereto. Such a mixture is called a composite sample. The usual methods of taking such samples at creameries and cheese factories dur- ing the past few years have been as follows: 1()!2. Methods of taking composite samples, a. Use of tin dipper. Either pint or quart !Mason fruit jars, or milk bottles provided with a cover, are used for receiving the daily samples. (_)ne of these jars is supplied for each patron of the factor^' and is labeled with his name or numlier. A small cjuantity of preservative (bi-chromate of potash, biclilorid of mercur3', etc., (see 172) is added to each jar; these are placed on shelves, or somewhere within easy reach of the operator inspecting and weighing the milk as it is re- ceived at the factory. When all the milk delivered by a patron is poured into the weighing can and weighed, a small portion tliereof, usually about an ounce, is put into the jar ialjeled with the name or number of the patron. The samples * Rullctin No. '.I, May ISllU. Comj^ositc Samples of Milk. 135 are conveuientl}' taken bj' means of a small tin dipper holding about an ounce. This sampling is continued for a certain number of days, a week, ten days, or sometimes two weeks, a portion of each patron's milk being added to his particular jar every time he delivered milk. Each of these composite samples are then tested; this test takes the place of separate daily tests, and gives accurate information regarding the average quality of the milk delivered by each patron during the period of sampling. The weight of Initter fat which each patron brought to the factory in his milk during this time, is obtained by multiplying the total weight of milk delivered during the sampling period b}' the test of the composite sample. 163. This method of taking composite samples has been proved to be practically correct. It is absolutely' correct onlj- when the same weight of milk is delivered daily by the particular patron. If this is not the case, the size of the various small samples should bear a definite relation to the milk delivered; one-sixteen hundredth, or one-two thousandth of the amount of milk furnished should, for instance, be taken for the composite sample from each lot of milk. This can easily be done by means of special sampling devices (see 165). As the quantities of the milk delivered from day to day by each patron varjr but little, perhaps not exceed- ing 10 percent of the milk delivered, the error introduced by taking a uniform sample, e. g., an ounce of milk, each time is, however, too small to be worth considering in fac- tory work, and the method of composite sampling described is generally adopted in separator creameries, and in cheese factories, where the payment of the milk is based on its quality. 136 Tci/ini^- Milk II ml /Is Pnnliirls. 1()4-. liy tliis method of compOHile sampling each lot of rich, medium or thin milk receives credit for the amount of butter fat which it contains, and com[)licalions that might arise trom testing only one day's milk at irregular intervals are avoided. Tn order to obtain rclialile results liy com- posite sampling it is essential that each lot of milk sampled shall be sweet and in good condition, containing no lumps of curdled milk or small butter granules churned out. The milk is of course always evenly mixed before the sample is taken. 1G5. b. Driji samjiJe. Composite samples are sometimes taken at creameries and cheese factories by collecting the milk that drips through a small hole or tube placed in the conductor spout through which the milk runs from the weighing can to the receiving vat or tank. A small portion of the drip is then each day placed in the composite sample jar, or the quantity of drip obtained is regulated so that all of it may be taken. In the latter case the i|uantily of milk delivered will I'uter into the composite sampling as well as its quality, and the sample from, say 10(1(1 Ihs. of milk will be twice as large as the sample Irom 5(10 lbs. of milk. Where it is desired to vary the size of the sub-samples, according to the ([uantity of milk delivered from day to day, it is necessary to adopt the method of collecting drip sam- ples, just ex|)lained, or to make use of special sampling de- vices, lik(^ the " milk thief," a Scovell sampling tube, etc. 'J'he principle of both these tubes is the same, and it will be sutTicient to describe here only one. 1 (■>(». c. The tScDvcll aiimpjiiuj tiihc:'^ This convenient de- vice lor sampling milk (tig. 41"), consists of a drawn copper '•■ Ki'MliH'li) rx j.iMiinriit s(;ili(.ii, sill rt'itort. ]tp. xxvi-.xxxii. Coinfositc Sin///>/cs of Milk. 137 or brass tube, one-half to one inch in diameter: it is "pen at l)otli ends, the lower end sliding snugly in a cap prii\'ided with three elliptical oi)enings at the side, llirough which the milk is admitted. Tlic milk to be sampled is poured into a cj-lindrical pail, or the factor}' weighing can, and the tulie, with the cap set so that the apertures are left open, is lowered into the milk until it touches the bottom of the can. The tube will be filled instantly to the level of the milk in the can, and is then pushed down, thereby closing the apertures of the cap and confining within the tube a column of milk representing exactly the quality of the railk in the can, and forming an aliquot part ihereof. The milk in the sanii)ling tufie is then emptied into the composite sample jar by turning the tube upside down. 1()7. If the diameter of the samjjliiig pail used is S inches, and that of the sampling tube I inch (these dimensions will lie found con\'enient in sampling milk from single cows), then the ]^ quantit}' of milk secured in the tube will always stand in the ratio to that of the milk in the pail, of [);) - to 8 ■-, * that is, very nearly 1 :2.5C; no matter how much or how little milk there is in the pail, the sample will repre- sent .;.',,; part of the milk. For composite sampling ol the milk of single cows, this proposition will prove about right; if more milk is wanted for a sub-sample, tlie milk to be sami *Tlie contents of a cvliiidcrare represented l.)_v the formula ,Tr'-li, r Ijeinf the radius oi the cylinder, and h its height. The relation between two cylinders ot the same height, the radii of which are R and r. is, therefore, as ;rR~h to ,Tr-'h, or as R- to r-. 138 Testing Milk and Its Products. sampled maj^ be poured into a can of smaller diameter. If the mixed milk from a number of cows is to be sampled, a wider sampling can is used. By adjusting the diameters of the tube and the can, any desired proportion of milk can be obtained in the sample. For factory sampling, with a 26-inch weighing can, a tube three-quarters of an inch in diameter will be found of proper dimensions. 168. The sampling tube will farnish a correct sample of the milk in the can, even if this has been left standing for some time; it is better, however, to take out the sample soon after the milk has been poured into the can, as the possible error of cream adhering to the side of the sampling tube is then avoided. 109. The accuracy of the sampling of milk bj' means of the Scovell tube was proved be3'ond dispute in the breed tests conducted at the World's Columbian Exposition in 1893, in which tests this method of sampling the milk pro- duced by the single cows, and the different herds was adopted.* The data obtained in these breed tests also far- nish abundant material proving the accuracj^ of the Babcock test. la using an}^ one of these tubes, the size of the sample is regulated tiy the amount of milk in the sampling can, as the milk always rises to the same height in the tube as in the can. In all cases cylindrical sampling cans must be used. 170. Composite sampling with a " one=third sam= pie pipette." Milk is sometimes sampled directly from the weighing can into the Babcock test bottle by means of a ^Kentucky experiment .station, 8tli report, pp. xx.\:-.\-xxi. Aoother form of a milk sampling tube in use at the Iowa e.xperiment station is descrilied and illus- trated by Mr. Eckles, in Breeder's Gazette, May 19, 1897. CoinJ^osiic Sanifilcs of Milk. 139 pipette boldinii' 5. ST cf, which is one-thinl the size of the regular pipette. This quantity is measured into tlie testhottle fromtliree successive lots of millv, and the test then made in the ordinary manner. In this wa}' one test shows the average composition of the milk delivered during three successive davs. or deliveries. When this method is adopted, as manj' test liottles are provided as there are patrons; there is no need of using an}' preservatives for the milk in this case. Fig. 42 shows a convenient rack for holding the test bottles used in composite sampling with a "one-third sample pipette." Accurate results can be obtained by this method of sam- pling, if care is taken in measuring out the milk, and if it is not frozen or contains lumps of cream. It is doubtful if the method has any advantage over the usual method of composite samp- ling. If milk is delivered daily and each lot is sampled with the one-third pipette, twice or three times the num- ber of tests are required as when composite sam- ples are taken and tested once every week, or every ten days. This method fur- thermore takes more time in the dail}' sampling than the latter, as the Fig. 42. Test bottle Tacli for use in creameries and cbeese factories. 140 Tci/iiig- Milk and Jls Products. quantity of milk must lie measured out accurately each time. Tl' the test Ijottle is accidentally- broken, or some milk spilled, the opportunity' of ascertaining the fat con- tent of the milk delivered during the three days is lost; if a similar accident should occur in testing composite samples collected in jars, another test can readily V)e made. 171. Accuracy of the described methods of sam- pling. An experiment made at the Wisconsin Ciiry School may hero be cited, showing that concordant results will be obtained by the use of the drip sampling method and the Scovell tube. Two composite samples were taken from fifty different lots of milk, amounting to about GOOO lbs. in the aggregate. One sample was taken of the drip from a hole in the conductor spout through which the milk passed from the weighing can; the other was taken in the weighing can by means of a Scovell sampling tube. The following percentages of fat were found in each of these samples: JSiihrock Ifsl. Griiri,„rlrii: rn:nhjsis. Drip cntn|K)site sample 4.0 ])ercent. +.04 percent. Scovell tulie composite sainj5le.. 4.1) " 4.06 " Preservatives for Co.mposite Sa.mj'LEs. When milk is kept for any length of time under ordin- ary cf)nditi(jns, it will soon turn sour and Ijecome lop- pered, and further decomposition shortly sets in, which renders the sampling of the milk both dillicult and unsatis- factory ( I It), 'flic changes which occur are due to the forma- tion of lactic acid through the action ol'l.)acteria on milk sugar; the acid in turn coagulates the casein of the milk, but does not destroy or attack the butter fat (I'T). The period dur- ing which milk will remain in an apparently swei^t, or fresh condition varies, with the temperature at which it is kept, Composite Sciiiiplcs of Milk. i^i and wilh the cleanliness of the milk, from less than a day to a week or more; milk will not generally remain sweet longer than two days at the outside, at ordinary summer or room temperature. In order to preserve comi)Osite samples of milk iu a proper condition for testing, some chemical which will check or prevent the fermentation of the milk must lie added to it. A number of substances have lieen proposed for tliis purpose. 172. Bi=chromate of potash. Of these, luchromate of potash is to lie preferred, in the opinion of the aiUhors, on account of its relative harndessness, its cheapness and efflcienc}'. The bi-chromate method for preser\'ing samples of milk was i)roposed hy ^Ir. J. A. Alen, citv chemist of Gothenbuig, Sweden, in ISII-!,* and has been generally adopted in dair}- regions in this country and abroad. While not perfectl}- harmless, the bi-chromate is not a violent poison like other chemicals proposed for this purpose, and no accidents are liable to result from its use; at least none have been known to the writers to occur during the j-ears that it has been used in creameries or dairies as a preserv- ing agent. 173. The (piantit}' of bi-chromate necessar_y for preserv- ing half a pint to a pint of milk for a period of one or two weeks is about one-half gram (nearly 8 grains). As there are about 000 half-grams in a pound, this quantity will (-uHice for nine weeks for a creamery having one hundred patrons, if tests are made once a week, or for three months (00 days), if tests are made every ten da)'s. According to Winton and Ogden,t a .22-inch pistol cart- * Biedermaiin's Centrallilatt. lS:r2, p. .119 tConnecticut experiment statiou repoit 1,SS4, p. 'I'll I42 Tcsliiii^- Milk and lis Products. ridge shell cut to .'. inch long, or a .P)2-inch calibre shell cut to \ inch long will hold, when loosel}' filled, enough of pow- dered bi-chromate to preserve i pint, a .32-inch calibre shell cut to .', inch long will hokl enough to preserve one pint. These shells maj' be convenientlj- handled by soldering on to them a piece of stiff wire to serve as a handle. The amount of bi-chromate placed in each composite sample jar would fill about half the space representing one percent in the neck of the Babcock milk test bottle. 174. The first portions of milk added to the composite sample jars containing the specified amount of lii chromate will be colored almost red, but as more milk is added, day b3r day, its color will become lighter yellow. The complete sample should have a light straw color; such samples are most easily mixed with acid when tested. If more bi-chromate is used, the solution of the casein in the acid is rendered difficult and calls for more persistent shaking. Bi-chromate can l)e bought at drug stores or from dairy sujjpl}' dealers at about od cents a pound, and will cost about 2.") cents a pound at wholesale. Powdered bi-chromate of potash should be ordered, and not crystals, as the latter dissolve onh' slowly in the milk. Farrington's bichromate tablets contaiu the correct quantity of preservative for a quart sample, and will be found convenient; price per l,(U)(l tablets, $2.00. 175. Other preservatives for composite samples. Among other substances recommended for use in butter or cheese factories as milk preservatives for composite samples are boracic acid compounds, formalin, chloroform, carbon bi- sulfld,* copper-ammonium sulfate, sodium Huorid, ammonia- * I>el;LW!in; c^pcriincnt ^^atinn, pit;litti report. IS'.Ki, wliirli also see for trials with a large iiiuuber uf dillereut preservatives. Composite Samples of Milk. 143 glycerin (sp. gr. l.((31), and mixtures containing mercuric clilorid (corrosive sulilimate) witli anilin color (rosanilin).* Ttie coloring matter in the latter compounds is added to give a rose color to the sample preserved, tlius showing that the milk is not fit for consumption; the bi-chromate giving naturally a yellow color to the milk, renders the addition of any special coloring matter unnecessarj'. None of the substances mentioned are as cheap as bi- chromate or more effective for the purpose for which the}' are used, when milk is to l>e kept not to exceed two or three weeks. The compounds containing corrosive sublimate are violent poisons, and must always be handled with the greatest care, lest they get into the hands of children or persons un- familiar with their poisonous properties; they will preserve the milk longer than bi-chromate when applied in sufficient quantities, but for factorv use the latter is amply effective, and has, as already stated, the advantage in several other respects. I'JO. Care of composite samples. The composite sample jars should be kept covered to prevent loss by evaporation, and in a cool place. They should be kept in the dark, in a special closed- closet, or at least out of direct sun- light: the chromic acid formed Ijy the reducing influence of light on chromate solutions i)rodiices a leather}- cream which is very ditficultly dissolved in sulfuric acid. A coating of white shellac will protect the labels of the composite sample jars so that thej- may be used for a long time, allowing the jars to be washed and cleaned after each period of testing. The shellac is applied after the names of the patrons have been written on the labels, and these have ^- Iowa expeiiment station, bulletins ii, 11, o2. 144 7'cs//iio- Milk and lis Prodiicls. l)een |nit on tlie jiirs. (luinmed laljels, lx2,V in., answer this piir|)(ise well. Tn keeping the milk from da}- to da}-, care should be taken tliat the cream forming on the milk does not stick to the sides of the jars in patches above the level of the milk. Unless the daily handling of the jars, and the adf milk should reiircsent the average quality of the various lots of milk of which it is made up. This will invai'ialily be true if a definite aliqnot portion or fraction of the dillerent lots of milk is taken. If the weights of, say ten dillerent lots of milk are a. 4.0 Iha. „..et. 4.0 4,:; 4. 1 4.4 lbs. in.."; II II Ill III ](i 3 IV V IV -. 2:i.s ',1 7 10, ,s " Total 1187 J. 14 4. 73* 'iC, " Total 1 187 237 Average True av'ge tnst.. 1 1 . 24 Average True av'ge test 4,20 4,22t 10, 11S7 ■ 1187 171>. The flgurcs given in the table show that when the difl'ereut lots of milk vary in test and weight, as in the first case, the correct average test of the 1187 His. of milk is not found b)' dividing the sum of these tests liy five, which would give 4.14 percent; l.iut the percentage which 5t!.l* (the total amount of fat in the mixed milk, in Ifis.) is of 11 ST 10 146 Testing Milk and lis Prodiicls. (the total amount of milk, in lbs.) is 4.73, and this is the correct average test of the mixed milk made up of the five different lots. lu the second case, the variations in both tlie weiglits of the different lots of milk, and their tests, are comparatively small, and both methods of calculation give therefore practically the same average test; but also in this case, the correct average test is found by dividing the total amount of fat l)y the total quantit}' of milk, making 4.22 percent, instead of 4.20 percent, which is tlie arithmetical mean of the five tests, the quantities of milk in the various lots do not enter into the calculation of the latter,* 180. The second example represents more nearly tlian the first one the actual conditions met with at creameries and cheese factories. As a rule the mixed milk from a herd of cows docs not vary more in total weight or tests, within a short period of time like one to two weeks, than the fig- ures given in this example. On account of this fact, sam- ples taken, for instance, with a small dipper may give per- fectly satisfactory results to all parlies. If the difl^erent lots of milk varied in weight and test from day to day, as shown in the first case, it would be necessary to use a " milk thief " or a Scovell sampling tube for taking the composite samples, as the size of the samples taken would tlien repre- sent an exact aliquot portion of each lot of milk (KiG). 181. A patron's dilemma. The following incident which occurred at the Wisconsin Dairy School creamery during the past winter, will further explain the difficulties met with in calculating the average tests of various lots of milk. « In Ihe experimeiil given on p. Kl, (he arithmi'lifal mean of tlie tesis given i O.l.'i percent, wliile tlie true average fat content of tlie milk is 4.8',l pcrccLt. Composite Samples of Milk. izj.7 The ■weckl3' composite sample of the milk supplied )\y a patron of the creamery from his herd of 21 cows tested 4.0 percent. One day the farmer brought to the creamery a sample of the morning's milk from each of his cows, and had them tested; after adding the single tests together, and dividing the sum bj' 21, he obtained an average figure of 5.1 percent of fat. From this he concluded that the aver- age test of the milk from his cows ought to be 5.1, instead of 4.0, and uaturall}- asked for an explanation. 182. The first thing done was to show him that while 5.1 was the cojTect average of the figures representing the tests of his twenty-one cows, it was not a correct average test of the mixetl milk of all his cows, as he had not con- sidered in calculating this average, the cjuantities of milk yielded by each cow; the following illustration was used: Cow No. 1 yield -5 lbs. of milk, test ^.r, perceot. = n.;i Ilis. ef Imtter fat. Cow No. 2, yield 6 lbs. ot milk, test .'i.O pereeot. = n x ll.s, uf biiiter fat. Total 31 llis. Sl.s.i; 1.2 lbs. 4 3 pereent. The two cows gave 31 lbs. of milk containing 1.2 lbs. of fat; the test of the mixed milk would therefore not be 4.3 percent (^^— ), but ''"^^"'° = 3.87 percent. If the fat in the mixed milk was calculated by the average figure 4.3 percent, 1.33 lbs. of fat would be obtained, i. e., ,13 lbs. more than the cows produced. In order to further demonstrate the actual composition of the mixed milk of the twenty-one cows, the milk of each cow was weighed and tested at each of the two milkings of one daj'. The weights and tests showed that the cows pro- duced the following total numljer of pounds of milk and fat; 14S 7\^stiiii^- Milk and Its, Prodiirls. Morning iiiilkin;^, 1 l.'i.S lbs. of milk, containinp; r>.17 lbs. of fat. Nij;lit milkiiiK, 1.30. 1) lbs. of milk, containing 4.9H lbs. of fat. riM ■ 11 i. • 1 5.17X100 , _,, . , The monunjT milk contained ~r, -5 — =4.;)b percent 01 1 1 o . ._> ,. , , ^, . , . .„ 4.98X100 ., „,, .<•+<. fat, and the night milk =.!.S0 percent 01 tat. " 130.9 ^ The average of 21 tests of morning milk was 4 8 percent, and of 21 tests of night milk, I!. 8 percent. Tlie sura of the morning and night milkings gave: mill;; 211.2 lbs. fat lfl.15 ll)s. The mixed morn'ug and night milk, therefore, con- , . , 10.1,5-100 ... , ,■ J- t rni ■ ■ .1 4- tamed ^ ,— =4.1 perci'iit ol fat. J his is the true aver- age test of the morning and night milkings of these twenty- one cows, as found by weighing and testing separatelj' the milk of each eow at both milkings, 183. The total milk was strained into a large can at the farm, both in the morning and in the evening, A sample of the mixed milk was taken in each case with a longdiandled dipper as soon as the milkings were finished. When the cans of milk were delivered at the creamerj', a sample of each was taken with a Scovell sampling tube. The tests of these four samples are given below, together with the re- sults from the individul tests. Morning miU;. Nighl milk. Sample taken at the farm, with dipper.. 4.4 percent, 3,S percent, Sai7ii>le taken at creamcr^^ with Scovell tube 1.,") percent. 3.7 percent. Calculated from wci;j:lits and tests of milk from each cow 4.5 jicrcent. 3.S percent. The ligures gi\'cn show that practically uniform tests were obtainc. Butter fat test and yield of butter. The Bab- cock test shows the amount of pure butter fat contained in a sample of milk or other dairy products. The butter ob- tained hy churning cream or milk contains, in addition to pure Ijutter fat, a certain amount of water, salt and curd. While an accurate milk test gives the total quantity of but- ter fat which there is in the sample of milk or cream tested, the churn cannot be depended upon either to leave the same amount of butter fat in the butter milk or to include the same amount of water, salt and curd in the butter at each churning. If a quantity of milk, say 3000 Lbs., be thoroughly mixed in a vat, and then divided into half a dozen equal portions, a Babcock test of the different lots will show the same per- centage of butter fat in each portion. If, on the other hand, each of these lots be skimmed, and the cream ripened in different vats and churned separately, the same weight of luittcr from each lot of 50f) lbs. of milk will not Ije olitained, even by the most expert butter maker, or if all the opera- tions of skimming, cream ripening, churning, sailing and butter-working were made as nearly uniform as possil)le. Careful operators can handle the milk and cream so that very nearly the same proportion of the fat contained in the CaJctilation of Biillcr and Cheese Yield. i6i milk is rerovcred in the butter in different chiirnings, but since the water in butter is lield mechanically, and is not chemically comljined with it, the amount retained by the butter is quite variable in different churnings, espcciall}' since the laws governing the retention of water in butter are bat imperfectly understood. I*)7. Variations in the composition of butter. As an illustration of the variability of batter in its composition, the analyses of the Initter made in the lireed tests at the World's Fair in IS'.Jo, may here be cited; the butter was in all cases made 1)3' as nearly identical methods and under as uniform conditions as could possibly be obtained by the skilled operators having this work in charge; the average composition of 350 samples of this butter, with upper and lower limits, was as shown in the following table. Composition of samples of butter, Worlds Fair, 1S9,). Average of .350 analy- ses Lowerand upper lim- its Percent. 11.57 8.63-15.00 Percent. 81.70 7B. .5.3-38. 26 Salt and asb. ^um of water, curd, salt and a^b. Percent. 15. .30 Analyses of fifty samples of creamery butter taken from the tubs ready for market at as manj- Wisconsin creameries, in 1890, indicated that no two of them were exactly alike in composition, Init varied within the limits given below.* ■ Wisconsin experiment station, bull. oG. 11 l62 Tcsliiiis Milk (iiul lis ProdurLs. Suinnuirij of analyses of Wisconsin creamery hutter. Water. Bulter fat. Curd. Salt and asti. Sum of water, .^alt and curd. Highest Percent, 17.03 '.1. 18 1^.77 Percent 87. .50 77.07 8;-:. 08 Percent. 2. 4.5 0. :% 1.28 Percent. 4.73 1 . 30 2.S7 Percent. 22. :i.-j The preceiling anal3-.ses show the coiuposilion of butter made at one place where ever}- possiltki effort was taken to produce a uniform product, and of butter made at flftj- dif- ferent creameries, where there wa.s more or less variation in the different operations ol' manufacture, and in the appli- ances and machiuerv used. The majority of the samples of butter anal^'zed wore, in either case, uaturall}' of, or very near, the average composition given, but since there is such wide varitttions in the coiuposilion of the batter made fiy the uniform methods adopted in the World's Fair breed tests, butter of a more uniform composition cannot be expected from the thousands of diflerent creameries and pri\'ate dairies which supply the general market with l.iutter. The analyses of the fifty samples of creamery Ijutter, o-iven aliovc, show that the content of the butter fat varied from 77 to over 87 percent, and according to the average of the analyses, 83 i)Oun'ls of Itutler fat was contained in, or made, 100 lbs. of butter. There was, therefore, in this case produced 1^0,5 percent more butter than there was liutter fat, since 83:1(10 : : 100 : .x : therefore lf)0,-: 100 8;^ X i-;o..>. Calc Illation of IJ II Iter and Cheese Yield. 163 IDS. "Overrun "of churn over test. The yield of butter is not, however, as a rule compared with the amount of butter fat contained in the butter, but with the total but- ter fat of the whole milk from which it was made. This "increase of the churn over the test" is what is generally called the overrun in creameries. The overrun obtained in ditferent creameries, or even in the same creameries at different times, will be found to ^'ary considerably. When the milk is accurately tested, and the butter well worked, this overrun will vary from 10 to 1(1 j.ier- cent; that is, if a cjuantity of milk contains exactly IfMJ lbs, of butter fat, as found by the l^abcock test or any other ac- curate methoilof milk testing, from 110 to 1 1(1 lljs. of liutter ready for market may be made from it. Variations will occur in the speed of the se|)arator, in the conduct of the ripening and the churning processes, and in the condition of the butter when the churn is sL<>pi)ed, e\en under the very best of care and attention to details; and abso- lutely uniform losses of I'at in skim milk and batter milk, or the same water content of the t)utter cannot, therefore, be expected. IIMK Factors influencing the overrun. Theoverruu is influenced by two factors: the losses of butter fat sus- tained in separating the milk and churning the cream, and the gain due to the admixture of water, salt, etc., in the manufacture of butter. Considering first the losses of fat in skim milk and butter milk, the separator usually, when run at normal capacit}', will leave the same percent of fat in skim milk, whether rich or poor milk is skimmed; an excep- tion to this may be found in separating rich milk having large fat globules, or milk from fresh milkers, in either of 164 Testing Milk and Its Products. which eases the large size of the fat gloliules occasions a more complete separation of fat by the centrifugal force. But generally, speaking, the statement holds good that the total loss of fat in separator skim milk is a factor of the quantity of milk run through the separator, rather than of its quality. It follows from this, however, that the relatice losses of fat in skim milk will var^- to some extent according to the quality of the milk separated. Selecting two extremes in the qualitj' of milk, 2.5 and 0.0 percent of fat, there will be found, say .2 percent of fat in the skim milk from either lot, provided the separator is not unduly crowded, and the separation is con- ducted under normal conditions in either case. But .2 per- cent fat makes 8 percent of the total fat in the poor milk ( -^^^ "''')' ^'^'^ oiily 3 percent of that in the rich milk. It takes 4000 lbs. of the 2.5 percent milk to furnish 100 lbs. of fat, and only 1666 lbs. of the 6 percent milk; in skim- ming the poor milk, a loss of .2 percent of fat is sustained in the skim milk from 4000 lbs. of milk, while in the rich milk a similar loss is sustained in the skim milk from only 166G lbs. of milk. The example gives an extreme case, and one not likely to be met with in practice. The range in the richness of the milk delivered by different patrons at the factory is usually within one-half a percent of fat. In such cases the propor- tion of fat lost in skimming does not vary much, e. g., in case of milks containing 3 5 and 4 percent of fat, and varia- tions in the overrun occurring when the proper care in skim- ming, ripening and churning is taken, are due, therefore, primarily to differences in the water content of the butter made (107). Calciilaliini of Bitltcr and Cheese I'ield. 165 200. The losses from ver}- poor, ver}' rich and average milk, as received at creameries and cheese factories, can be traced from the following statement; this gives the cjuantitics of fat lost in handling milk of four grades, viz: 2.5, 3.5, 4.0 and CO percent, in case of each grade calculated to a stand- ard of 100 lbs. of fat in the milk. To supplj' 100 lbs. of fat would require the following amounts of the ditferent grades of milk: 4000 lbs. of milk testing 2.5 percent will contain 100 lbs. of fat. 2857 " " " 3,5 ' IOC 2500 " " " 4.0 " " " 100 " " 1666 ■' " " 6.0 100 " " Assuming that the skim milk contains .2 percent of fat and makes up SO percent of the whole milk, and that the butter milk tests .?> percent, and forms 20 percent of the whole milk, the butter fat record of the quantities of differ- ent grades of milk containing 100 lbs. of fat will appear as follows; Fat available for butter in different grades of milk. Grade of milk. WholH milk. Skim milk. Butter milk Total loss. Fat a\ aihit,le lor t..uHer. 4000 til. 2, ,5 per et. 3200 It.. . 2 per et. ,-(00 It.. , .3 per et. 8.8 6.3 5..> 8.0 I'ereent. Fat. 1(10 lb, 28,57 It., 3,,"> pL-r rt. HA It-.. 22,s,'i It.. . 2 per et. 2.4 It,. ,172 It., ,3peret. 91 " Fat 100 It., 2.5110 It., 4 per et. 4.(1 It., 2OO11 Ih, .2 per et. 1.7 It., ,500 11.. ,,3peret. 4.0 percent... Fat 100 11., icr.i;- , It.. 6 p-rct. 4.0 It., i:;3:i It., .2 perct. !..■> 11. 333 11., ,3 per et. W,.> Fat, 100 It., •2.6 It., 1.0 It., l66 7<\s//wi,'- Milk and Jls Producls. The table shows that with 2.5 percent milk, there is a loss of (i. 4 lbs. of Irtt in the skim milk aii;e test ol the niiUx is o.s percent. ,'i4-0 lbs. of butter was made from tliis milk, as shown liy the wei;;hts of the packed tubs. Hv a simple muUI]»lication we find th.at the ndlk contained SOOdX .•18S=304- His. ot butter fat. The (liherence be- tween the wei,L;ht of butter anil butter iat, is, therelore, 36 II3S.; oCiXllio ?>{'< is ~ -=tt.S percent ot the iiuantitv ot liutter lat m the milk; that is, the overrim l(U' thedav considered was 1 1.-'-; percent. The formula for the ovcj-niti is as follows: _ lb— t) TOO X — ^ h and /' designating the quantities of lintter and butter fat, respectively, made from or contained in a certain quantity of milk. In the preceding example, the calculation would be as , ,, i.'UO— 3041 too , , ^, . follows : =1 1.^ i)ercent. 304. ' '20'^. Conversion factor for butter fat. A committee of the Association of American Agricultural Colleges and Experiment Stations reported at the ninth annual conven- tion of the Association that " in the ninet\--day Columbian Dairy Test, iHJ.dT percent of the fat in the whole milk was recovered in the butter. This butter on the average contained Si'. 37 i)ercent butter fat; in other words, 117.3 pounds of but- ter were made from each loO pounds of butter fat in the whole milk.* The exact conversion factor would be 1.173. * When .S2.:;7 Ills, of biutor fat will make 100 lbs. of Lmlter, liow luueh Imtter will '.ti'i 07 Ills, oi butter fat make ? .s_'.;;7 i ;iG.0,7 : : InO: x, i = 117 Z. 1 68 yoV///-,' J\lilk and J is Prudurls. As this is an awkward imraber to use, and as 1,1 is so nearly the same ... it has seemed best to recomuiend that the latter be used as the eonversion faetor." A resolution was adopted by this association recommend- ing that the approximate e(|uivalent of batter be computed ))y multiplying the amount of butter fat by 1,',. These figures represent more than ordinary care in testing, skimming and churning, and probably the minimum loss of fat in the manufacturing processes. The increase of churn over test represented by one-sixth, or 10 percent, therefore may be taken as a maximum " overrun." Butter makers who report overruns of 1(1-20 percent do not show their expert- ness in butter making by such high figures, but their lack of accuracy in testing, or carelessness in working the butter; a large overrun may be oljtained both by reading the test too low, and by leaving an excess of water in the butter thi-ough insudicient working or other causes. 2(H{. Butter yield from milk of different richness. a. Use of huttcr chart. The approximate yield of butter from milk of dittbrent richness is shown in ta))le IX in the Appendix. This table is founded on ordinary creamery ex- perience and will be found to come near to actual every-day conditions of creameries where modern methods are followed in the handling of the milk and its products. The table has been prepared in the following manner: It is assumed that the average lossof fat in the skim milk is .20 iiercent, and that 85 lbs. of skim milk is obtained from each 100 lbs. of whole milk; to this loss of fat is added that taking place in the butter milk; about 1(1 lbs. of butter milk is obtained per 100 lbs. of whole milk, testing on the average .30 percent. Ciih Illation of Butter and Cheese J'iehi. 169 If / designate the fat in 100 lbs. of milk, then the fat recovered in the butter from lOO lbs. of milk will be There is, on the other hand, an increase in weight in the butter made, owing the admixture of non-fatt}' components therein, principall)- water and salt. Butter packed and ready for the market will contain in the neighborhood of 81 percent of fat (197), so that the fat recovered in the but- ter must be increased by '„"" = 1.10. If B therefore desig- nate the yield of butter from 1(10 lbs. of milk, the following formula will express the relation between j'ield and fat con- tent, provided there are no other factors entering into the problem, viz: B = (f — .20) 1.19 Certain mechanical losses are, however, unavoidable in the creamery, as in all other factory operations, viz: milk and cream remaining in vats and separators, butter sticking to the walls of the churn, etc, These losses have been found to average about 3 percent of the total fat in the milk handled, under normal conditions and under good manage- ment (202); we therefore deduct this percent from the pre- ceding value for B, and have: ]!=(f— .2(1)1.16 204. Table IX in the Appendix, founded on this formula, may be used to determine the number of pounds which the milk delivered by the various patrons within the limits of 3 and 5.3 percent will be likelj- to make. It presupposes good and careful work at the separator, churn and butter worker, and will generallj' under such conditions show yields of butter varying but little from those actuallj' obtained. It may also ))e conveniently used liy the butter maker or 170 Tcsliii'j; Milk (iiid Its Produch. the niuiiagei' to check the work in the crcarnery; the aver- age test ijf the milk received daring a certain period is Ibund bj' dividing the total hutter fat received by the total milk, multiplying by Idl); the amount of butter which the total milk of this average fat content will make according to the table, is then compared witli the actual churn yield. lixample. A creamery receives 200,000 llis. of milk during a month; tlie milk of each patron is tested and the fat contained therein calctilatcd. The sum of these amounts of fat may be 75S3 Iti?.; the average test of the milk is then 3.70 percent. Ac- cording to table IX, 10,000 lbs. of milk, testing 3.8, will make 421.2 lbs. of Imtter, and 200,000 lbs., therefore, «l-2-t lbs. of Init- ter. The total f|unntit_v of butter made during the month should not vary appreciably from this figure. 205. b. Use of overrun table. The table referred to al)0ve gives a definite calculated butter 3'ield for each grade of milk, according to the average creamery conditions. As it may be found that this table will either give uniformly too low or too high results, table X in Appendix is included, bj- means of which the butter yield corresponding to overruns from 10-20 percent may be ascertained in a similar way as above described. The total yield of butter is divided by the total number of pounds of fat delivered; the quotient will give the amount of butter made from one pound of fat, and this figure mul- tiplied by the fat delivered by each patron shows the pounds of butter to be credited to each patron. To use the table, find in the upper horizontal line the number corresponding most nearly to the numl)er of pounds ot butter from one pound of fat. The vertical column in which this falls gives the pounds of butter from 1(10 lljs. of milk containing the percents of fat given in the outside columns (Babcock *). *\Voll, llaDdbook for I''ariuers and Dairyiuen, p. 27;i. Cdlciilalioii of J ill tic I' and Cheese 1 lehL 171 1!, CaLCII l,,\TII)N OF Yir.l.K OV ClIlOESE. ■'OU. :i- From fat. The appropriaLc yielil ol' gi-een cIilmI- (lar clieesc IVdm 101) l^)s. fif milk may lie f'uuiKt liy nuilti|ily- iiig the percent of fat in tlie milk by l',7: if /' (iesignate the percent of lat in the milk, the torniula will, thei'elbre, be: Yield .if cheese = 2 7 f (I) The factor 2.7 will only liohl good as the average of a large number of cases. In extensive in\estigations during three consecutive vears, \-a\\ .^h'ke """ found that the numlierof pounds of green cheese ol.)taineiI for each iiound of fat in the milk varied from 2.51 to o.Oii, the average ligiires for the three years 1892- '1)4, inch, lieing 2.7o, 2.71, and 2.72 11)S., respectively. The richer kinds of milk will prddace cheese richer in I'at. and will yield a relatively a larger quantity of cheese, pound for pound, than poor milk, lor the reason that an increase in the fat content of milk is accompanied b}- an increase in the other cheese-producing solids of the milk.t The preceding formula would not, therefore, be cor- rect for small lots of either rich or poor milk, but only- for milk of average composition, and for large (piantities of normal factory milk. For cured cheese the factor will lie somewhat lower, viz: about 2.(), on the average. 207. h. From solids not fat and fat. If the percent- ages of solids not fat and of fat in the milk are known, the following formula by Babcock will give close results: Yield of green cheese = L.'iS (|..'.nf) . . (II) *N. Y. exitijriment statiuo, (Geneva), luiUeliiis No. 03 and S'_', t iDvesti^'iitiuus as to tlie relation l.>etween the qualily of the miik and tlie yield of cheese have heen conducted by a number of exjt^rinifiit stations; the fol- lowing references give the main contributions ])ublished on this point: N. Y. (Geneva) ex]!. sta., reports 10-13, incl.; Wis. exjj, sta., reports 11 and 12; r*nt. A;j;r. College, reports lS9-I-'96, inch; Minn, exp, sta., reports ISDJ-'Ot, incl ; lou'a exp- sta , bull. 21. 172 Tcsliug Milk and Its Products. s being the percent of solids not fat in the milk, and / the percent of fat. Tlie derivation of this formula is as given Ijelow.* Cheese is made ^ip of water, fat and casein, with small quanti- ties of other milk solids, and also salt and a little cheese color ad- ded in the process of manufacture. The green cheese as taken from the press, will contain in the neighborhood of 37 percent of water. t The total c|uantity of solids other than fat in the cheese has been found equal to one-third of the solids not fat in the milk from which the cheese is made. The fat contained in the cheese is that found in the milk less the fat lost in the process of manufacture, i. e., in the ■whey and the drippings. In the manu- factiire of cheddar cheese very nearly 9 percent of the fat in the milk is lost ; a little less than 9 percent has been found in case of very rich milks, and a trifle more with poor milks, but this may be considered a correct average figure. The quantity of cheddar cheese made from 100 lbs. of milk will therefore be found by add- ing one-third of the percent of solids not fat in the milk to 91 per- cent of the fat, and multiph'ing the sum by V;,", or 1.58. The for- mula then is Yield of green cheddar cheese from 100 lbs. of milk = 1 .58 (-^ + 91 f. j The solids not fat can be readily' ascertained from the lac- tometer reading and the percent of fat, as shown on p. ST, b}' means of table V, given in the Appendix. Table XI in the Apipendix gives the j-ield of cheese from 100 lbs. of milk containing from 2..') to CO percent fat, the lactometer readings of which range between 20 and 30. By means of this table cheese makers can calculate very closely the yielils of cheese which certain quantities of milk will make; as it takes into consideration the non-fatty solids as * AVisconsin exiterimciit atation, Lwelftli report, p. ]i).5. t N. Y. (Geneva) experiment station, twelfth report, p. iKj. Calculation of Btdtc7' and Cheese Yield. 173 well as the fat of the milk, the results obtained by the use of this formula will be more correct than those found by means of formula (I). The uncertain element in the for- mula lies in the factor 1.5S, which, as shown above, is based on an average water content of 37 percent in the green cheese. This maj', however, be changed to suit anj- partic- ular case, e. g., 35 percent (',;"," = 1.54), -fO percent (',"" = l.Cu) etc. The average percentages of water in green cheese found by Van Slyke in his investigations referred to above, were for the years 1892-'94:, respectivel}', 36.41, 37.05 and 36.70 percent. 208. c. From casein and fat. If the percentages of casein and fat in the milk are known, the yield of cheese may be calculated by the following formula, also prepared bjr Dr. Babcock: Yield of cheese = 1.1 f + 2.5 casein .... (Ill) This formula will give fairlj' correct results, but no more so than formula (II) ; it is wholly empirical. 174 Testing- Milk and fis Products. ClIAPTKIt XIII. CALCULATING DIVIDENDS. A. — CALoiJr.A'i'iN(; Dividends at Chka-Meriks. '2(10. Tlie simi)lest method of calculating dividends at creameries is to lind the weight of l^utter fat in pounds delivered to the creamery by each patron for a certain length of time, and then miiltipl3' this amount l)y the price l^er pound of i'at. h'armers arc usually paid once a month for their milk at the factoiy. Each lot of milk is weighed, when delivered at the creamer^-, and a small quantity thereof is saved for the composite sami^le, as previously explained under composite tests (134). Some creameries test these samples at the end of each week, and others after collecting them for ten days or two weeks. If the four weeklj- composite samples of a patron's milk tested 3.S, 4.0. .'!.li, 4.1 percent., these four tests are added together, and the sum diviiled b}' 4; the result, 3.75 percent, is used as the average test of this milk. B}- multiplying the total number of pounds of milk delivered b}* this patrf)n, by his average test, the total weight in ptounds of butter fat delivereil to the factoiy during the month is olitained. This weight of fat is then multiplied by the price to be paid by the cream- ery per pound of butter fat; the jn'oduct shows the amount of money due this [latron lor the milk delivered during the time samples wei'c taken. 'nilO. Price per pound of butter fat. The method of obtaining the price to be paid for one pound of butter Ci-iJciihitiiig Dividends. 175 fat varies somewhat in different creameries, on account of the various ways of paying for the cost of manufacturing the butter. The method to be Ibllowcd is generally deter- mined by agreement lietween the manufacturer and the milk |)roducers, in case of proprietar}' creameries, or be- tween the shareholders, in co-oi)erative creameries. The following methods of paying for the cost of manufacture are at the present time met with in American creameries: vll. I. Proprietary creameries, a. When the cream- erj- is owned b}' some one person or company, the owner or owners agree to make the butter for 3 or 4 cents a pound; the difference between the total receipts of the factory and the amount due the owner is then divided between the dif- ferent parties, according to the amount of butter fat con- tained in the milk delivered by them. In the majority of cases, the price charged for making butter is now f cents a pound; 'XI and 3,V cents are sometimes charged. The larger the amount of milk receiveil at a fac- tor}', the lower will naturally be the cost of manufacturing the butter."" I). The proprietor of the creamery sometimes agrees to pay a certain price for 1(10 lbs. of milk delivered, acc^ording to its fat content, the price of milk containing 4 percent of butter fat being the standard. This price may change during the different seasons of the 3'ear li_y mutual agreement. c. A creamery owner ma}- offer to pay 1 to '1 cents, usually 1-^r cents, below the average market price of butter, for each pound of butter fat received in the milk. 'wl'2. II. Co-operative creameries. In this case, where the creameries is owned by the patrons, one of the stockholders ■ ^ViseoDsin experiment staUou, bull. .7pii. p. 2iJ. 176 Testing MUk and Its Products. who is elected secretar)' attends to the details of running the factory and selling the product. His accounts show the amount of monej' received each month for the butter and other products sold, and the expenses of running the factorj' during this time. The expenses are subtracted from the re- ceipts, and the balance is divided among the patrons, each one receiving his proportionate share according to the amounts of Ijutter fat delivered in each case (as shown by the total weight and the average tests of the milk delivered during this time). In nearly all cases, the farmers receive about eight}' pounds of skim milk for each hundred pounds of whole milk they deliver to the factory, in addition to the amount received for the milk, calculated according to one or the other of the preceding methods. 213. Illustrations of calculations of dividends. In order to illustrate the details of calculating dividends, or the amount to be paid each patron for the milk supplied each month, when payments are made by each of the four systems given, it will be assumed that a creamery receives 5000 pounds of milk daily for thirty days, and makes (1050 lbs. of butter from the 150,000 lbs. of milk received during this time. The average test of this milk maj' be found bj' multiplying the total weight of milk delivered by e.ach pa- tron by his average test, and dividing the sum of these pro- ducts by the total weight of milk received at the creamery, (in the example given, by 150,000) the quotient being multi- plied by 100. Such calculations may show that, e. g., 5700 lbs. of butter fat have been received in all in the milk delivered by the different patrons; this multiplied by 100, and divided by 150,000 gives 3.8 as the average test, or the CdlcKhltlili;' JJll'iilcihh. • 7 average amount of butter fat in each HHi Ihs, of milk re- ceived during the month. So far. the method of calculation is common for all differ- ent S3'stems of payment given above; the manner of pro- cedure now differs according to the agreement made between owner and patrfins, or between the shareholders, in case of co-operative creameries. 21-i. I a- If the net returns tor the MuM Wy-i. of flutter sold during the month were .^IlUT, and the creamerv is to receive 4 cents per pound of butter as the cost of manufac- ture, etc., the amount due the creamery is iiG50>;.04^S2(i(), and the patrons would recei\'e $1 197 — s2(iG=|0:n , This sum, $031, is to be paid to the patrons for the .'iTnil lbs. of butter fat, which, as shown above, was the weight of fat contained in the 150,000 Dis. of milk deliAered during the month. The price of one pound of butter fat is then easily found: $9ol^-5T00=l(;.'j cents. This price is then paid to all pa- trons for each pound of butter fat delivered in their milk during the month. The monthly milk record of three pa- trons ma3', e. g., be as given in the following table: FIRST WEEK SECOND WEEK TniRD A\EEK FOURTH WEEK Total Milk lbs. 1 PATRON. Milk lbs. Test perct. Milk Test lbs. per ct. Milk lbs. Test per or. Milk Test lbs perct. No. 1 7011 ■M.SU 3000 3. .5 66.5 1 :^.,■^ 2000 ' 3.S :^600 720 3.63 .3.6 J.O :.M.iO 7.50 1,500 3.45 1 3, 550 3.55 S.73 " '^v 3. 6 . 7,830 s.n ^lultiplyiug each patron's total milk by his average test gives the number of pounds of butter fat in his milk, and this figure multiplied by Itb.J shows the money due for his milk, as given below. 12 i^S Testing Milk and Its Products. PATRON. Total milk lira. Avorit[(e test, per eont. :i..55 ;i.7 ;i9 Butter fat. llw. Prioe of fat per Il».s., cetllrt Arnoiiots due. No, 1 13,,5.i0 2, Si:.5 7,8.50 jm.o 1114.. 5 :!0.i.4 HIV, l''':i $78. .W J 11. 7.5 48. '.)i; " 9 '■ 3... . 215. b. When the proprietor of a creamer^' agrees to pay a certain price for 100 lbs. of 4 percent, milk, tlie receipts for butter sold and the price per pound of Ijutter do not enter into the calculation of the amount due each patron for his milk; Vjut the weight and the test of each patron's milk are just as important as before. If it is agreed to pay 6(j cents per 100 llis. of 4 percent milk (i. e. milk con- taining 4 percent of butter fat), the price of one pound of but- ter fat will be G(j ^4 = 101 cents, and the amount due each patron is found by multiplying the total weight of l;utter fat in his milk by this price. To facilitate this calculation,, so-called lidativeValve Tables have been constructed, the use of which is explained in the following (1'2]). 21G. c. If a creamery agrees to pay for butter fat, say 1 'r cents per pound below the average market price olitained for butter each month, the price of one pound of butter fat is found by averaging the market quotations and subtract- ing 1^ cents therefrom. IE the four weeklj' market prices were 171, 17, 16.} and 19 cents, the average of these would be 17J, cents, and this less H- cents gives 10 cents as the price per pound of fat to be paid to the patrons; this price is then used in calculating the dividend as in case of method I a. I'atroii No. Total m Ik lbs. ::, r,r,n 2, 82.T 7,x:'.i) Averat^e test, per ceul. Butter fat, 11.3. Price per lb., f fat. ;ents. Amounts due. 4.M.I1 nil. 5 :'.! 1.1,4 tr, S7(;.% iri.72 48.86 CalculatiuQ- Dividends. 179 217. II. If the creamer}' is owned b)' the farmers, the running expenses for a month are subtracted from the net returns received for the butter, and the amount left is divided by the total number of pounds of l)utter fat deliv- ered during the month, to get the price to be pjaid per pound of butter fat. This price is used for paying each patron for his milk according to the amount of fat contained therein, as already explained under Proprietary Creameries (■214). The monthlj' running expenses of a co-operative creamery generallj' includes such items as the wages of the butter maker (and manager or secretar}', if these officers are sal- aried), labor (hauling, helper, etc.), cost of butter packages, coal or wood, salt and other supplies, freight and commis- sion on the butter sold, repairs and iusurance on liaildiugs, etc. A certain amount is also paid into a sinking fnnd, which represents the depreciation of the property, wear and tear of l)uilding and machinery, bad debts, etc. These items are added together, and their sum subtracted from the gross receipts for the butter sold during the month. 218. Assuming the receipts for the butter sold during the month to be $1197, and the running expenses of the factor}' .1285, the amount to be divided among the patrons is $912, the quantity of butter fat received was 5700 lbs., and the price per pound of butter fat will therefore be 1 6 cents. The account will then stand as follows: Patron No. Total milk Average test, per cent. Butter fat, lbs. Price per lb. of butter tat, cents Amounts due. 1 ■2 8 13, Kn 7,830 3. .5,5 8.7 3.9 48 1 . int.. 5 3M5. 4 IB Hi $7fi.9ci IB. 72 4S.8G iSo Testing- Milk (Did Its Products. '2111. Other systems of payment. IJesidcs these four systems of pajmeiU, there are various other agreements made between manufaeturer and producer, but witli them all the one important computation of the price to Ije paid per pound of butter fat is the basis of calculating the factory dividends, when milk is paid for by the JJabcock test. 220. Poying for Ivtter dcliierejl. In some instances patrons desire to receive pay for the quantity of butter which the milk delivered by them will make. This can be ascertained quite accuratel\- from the total receipts and the total weight of Ijoth butter fat and butter. The total money to Ije paid for butter (the net receipts) are divided by the number of pounds of butter sold, to get the price to be paid per pound of butter; the total yield of butter divided by the total amount of butter fat delivered in the milk, gives the amount of butter corresponding to one pound of butter fat, and the pounds of fat delivered b}* each patron is then multiplied by this figure. This method requires more figur- ing, than those given in the preceding, and the dividends are no more accurate, in fact, less so, than when calculations are based on the price per pound of fat. 221. Relative value tables. These tables give many of the multiplications used in computiug the amount due for various weights of milk testing from 3 to 6 per cent, of fat. They can be easily constructed by any one as soon as the price of one pound of fat is determined in each ease. If the price to be paid per pound of fat is, say, 1 5 cents, the value of each KIO lbs, of milk of different q\iality is found by multi plying its test by 15. It the average tests of the diflerent patrons' milk vary from 3 to "i percent, the relative value table would be as follows: Calcuhilliio- JDiz-idoids. l8l 3.0 X 15 = 45c. per 100 lbs. .-^.G X 15 = 54c. ]jer 100 lljs. 3.1 X 15 = 46. 5c. " 3.7 X 15 = 55.5c. 3.2Xl5 = 4Sc. " 3.8X15 = 57c. 3.3 X 15 = 49. 5c. " 3.9 X 15 = 5S.5c. 3.4 X 15 = 51c. " j 4.0 X 15 = 00c. 3.5 X 15 = 52.5c. " I etc. Continuing tliis multiplication, or adding the multiplier each time, in the same way for each tenth of a [)ercent, up to 5 percent, of fat, gives a table that can be used for calcu- lating the amount due per 100 lbs. of milk, at this price per pound, and the weight of milk delivered by each patron is multiplied by the price per 100 lbs. of milk shown in the table opposite the figure representing his test. Example. A patron supplies 2470 lbs. of milk testing 3.2 per- cent of fat; price per |)onn(l of fat 15 cents; he should then receive 24.70 X 4Sc.^$ll.s5, (see above table). Another patron deliver- ing 3850 lbs. of inilk testing 3.8 percent, will receive, at the same price per pound of fat, 38.50 X 57c. = $21.94. The relative value tables in the Appendi.r give the price per 100 lbs. of milk testing lietween 3 and li percent fat, when the price of three percent milk varies from :iO to 00c. per 100 lbs. In using tlie tables, first find the figure show- ing the price which it has been determined to pay for 100 lbs. of milk of a certain quality, say 3 or 4 percent milk; the figures in the same \'ertical column then give the price to l)e paid per 100 lbs. of milk testing between 3 and percent. Example 1. ft has been decided to pay 90 cents ])cr f 00 lbs. of 4 percent milk. The figure 90 is then sought in the table in the same line as 4.00 percent, and the vertical column in which it is found, gives the price per 100 lbs. of 3 to ]]ercent milk. 3.8 percent milk is thus worth 85 cents per 100 lbs., and 4.5 percent milk, $1.01, under the conditions given. The prices of other i|ualities of milk are found in the same wav. lS2 TcsLhio- Milk and lis Products. Example 2. In the example referred to under Illustrfitions of Calculating Creamery Dividends (I b, 215), the figures for the patrons No. 1, 2, and 3, would be as follows: Patron No. Milk delivered Ih3. Average te.st. Prii of e per 100 lbs. tiiiik, eents. A mounts due. ] 13.5.50 2325 7830 3. 55 3.7 3.;i 5R. 5 61. OJ. $79. 20 17.23 3 .50. 11 B. CaLUULATINC DlVUlE.NDS AT ClIEESE ]''aCTOKIES. 222. The amount of cheese made from a certain rjuantity of milk depends, as liefore shown (20(;j, in a large measure on the richness of the milk in butter fat. llich milk will give more cliesse per hundred weight than poor milk, and the increased j'ields will be nearl}-, Ijut not entirelj', propor- tional to the fat contents of the different kinds of milk. Since the quality of the cheese produced from rich milk is better than that of cheese made from thin milk, and will de- mand a higher price, it follows that no injustice is clone by rating the value of milk for cheese production bj' its fat content. This subject has been discussed frequontl}- during late years in experiment station publications and in the dairy press (see 20(J). Among others, Babcock has shown that the price of cheese stands in a direct relation to its fat content.* I-'rof. Robertson, the Jiairy Commissioner of Canada, is authority for the statement that the quality of the cheese made from milk containing li.O to 4.0 percent of fat was increased in value by one-eighth of a cent for every two-tenths of a percent of fat in the milk,t a figure which is fully corroborated by Dr. Babcock's results. The injustice f^ Wisconain ex[). station, I 111) r(|iort, p. 134. tlloard'.s Dulryriiaii, Mareli 20, IS'.lo. Cc//r///c/////i;- DiiiJrinh. I S3 of the " ijooling s^ysteui," ])y which all kinds of milk receive the same price, is ex-ident fn^m the preceding; if the milk of a certain patron is richer than that of others, it will make a higher grade of cheese, and more of it, per hundred weight, and hence should be paid a higher j^rice. Payment on the basis of the fat content of milk is, there- fore, the most equitaljle method of valuing milk for cheese making, and in case of patrons of cheese factories as with creamery patrons, dividends should be calculated from the results obtained by testing the milk delivered. The testing may be convenientlj' arranged b}' the method of composite sampling, in the same way as already- described for cream- eries (1112). *2'2;{. Calculation of dividends. As with creameries the first thing to be ascertained is the price to be paid per pound of luitter fat. The factory records should show the number of pounds of cheese made from the total milk de- livered to the factory during a certain time, generally one month, and the mone}- received for this cheese. The cost of making cheese and all other expenses that should be paid for out of the money received for the cheese are deducted from the total receipts, and the difference is divided among the patrons in proportion to the amounts of Initter fat de- livered in the milk. The weights of the milk delivered, and the tests of the composite samples furnish data for calculating the quantities of butter fat to be credited to each patron. The mone}^ to be paid to the patrons is then divided by the total weight of butter fat delivered to the factorj', and the price of one pound of fat thus obtained. The money due each patron is now found by multipl3'ing the total numfier of pounds of butter fat in his milk by this price per pound. 184 Testing Milk and Its Products. The illustrations already given for calculating patrons' dividends at creameries according to the various methods, will serve equally well to show the manner in which divi- dends are calculated at a cheese factory. For the sake of clearness an example is given that applies directly to cheese factories. 224. Illustrations of calculation of dividends. It may be assumed that 15,000 lbs. of green cheese is made from 150,000 lbs. of milk delivered to a factory in a month; according to the weighings and the tests made, the milk con- tained 5700 lbs. of butter fat. If the cheese sold at an average price of *lh cents a pound, the gross receipts would be $1,125.00. The amount to be deducted from the gross receipts will depend on the agreement made between the factory operator and the patrons, in case of proprietary cheese factories, or between the shareholders and the maker, when the factory is run on the co-operative plan. As be- fore we shall consider these sj'stems separatelj'. 225. I- Proprietary cheese factories. The owner of the factory generally agrees to make the cheese for a certain price per pound, and to pay the patrons what is left after deducting this conclusion. If the price agreed on is ],} cents per pound of green cheese, this would amount to $225, in the example given. Subtracting this sum from the gross receipts, .f] ,125, leaves $900, which is to be paid the patrons. The total amount of butter delivered hy the patrons was 5700 lbs.; hence the price of one pound of l)utter fat will be 900 ^-5700 = .] 577, or 15.S cents. Taking the figures for the three patrons already mentioned under creamery divi- dends, we then have: Calcitlaii)ig Dividends. 185 Patron. Total milk. No. 1 ; 13, S.W No. 2 2,825 No. 3 7,830 206. II. Co-operative cheese factories. The method of payment at co-operative cheese factories i.s nearly the same as that already given, except that a certain sum represent- ing the expenses is subtracted from the gross receipts for the cheese, and the balance is divided among the patrons according to the amounts of butter fat furnished by each, in the same manner as in the above case, after the price of a pound of fat has been obtained. The price per 1(MI lbs. of milk can be calculated in the the same way as at creameries, by multiplying the test of each lot b}' the price per pound of fat. 1 86 Tcsli)iu Mi7k (I lid /Is Products. CHAPTER XIV. CHEMICAL ANALYSIS OF MILK AND ITS PRODUCTS 227. An outline of the methods followed in determining quantitative!}' the components of milk and its products is given in the following, for the guidance of advanced dairy students. This work cannot be done outside of a fairlj- well- equipped chemical liboratorj', or by persons who have not been accustomed to handling delicate chemical apparatus and glassware, anal3'tical liah\nces, etc., and who have not a knowledge of at least the elements of chemistry and chem- ical reactions. A. — Milk. 208. In a complete milk analysis, the specific gravit3'of the milk is determined, and the following milk components, water, fat, casein and albumen, milk sugar, and ash. The methods of analysis descril)ed in the following are those used in the chemical laboratory of the Wisconsin experiment station, which in the main are the same as those adopted by the Association of (Jfflcial Agricultural Chemists, and in use with but slight modifications in the chemical laboratories of all ^Vmcrican experiment stations and schools.* 220. a. Specific gravity is determined bj' means of a picnouieter or specific gravity bottle, since more accurate * Tlie mttliods of analy.sls adoptt^d )iy the Asso. of Official Agr'l Chemista are published annually by the chemical division of the U. S. Department of Agricul- ture; see Bull. No. 46, Washington, 1.S95, edited by Harvey W.Wiley, secretary, l,p. H4. C/icniicnl ^\iuil\'sis of i] J ilk and Its Pi'odiicis. 187 results will thus l)e reached than \)S using an ordinary QaoA-enne laetometer. A thermometer is ground into the neck of the specific gravitj' bottle so as to form a stopper, and the bottle is provided with a glass-stoppered side tube to furnish an exit for liquids on expanding. A specific gra^'it^y liottle holding 100 grams of water is preferably used. The empty and scrupulousl)' cleaned bottle is first weighed on a chemical balance. The Ijottle is then filled with re- centl_y-boiled distilled water of a temperature below 60" F. (15. fi-' C.); the thermometer is inserted, and the l)0ttle is warmed slightly b}' immersing it for a moment in tepid water and left standing until the thermometer shows (iO' F.; the opening of the side tube is then wiped off and closed with the stopper, and the water on the outside of the bottle and in the groove between its neck and the thermometer wiped off with filter paper or a clean handkerchief, when the bottle is again weighed. The weight lieing recorded, the l)ottle is emptied and dried in a water oven, or, if sullicient milk is at hand, the ftottle is repeatedly rinsed with the milk, the spe- cific gravity of which is to be determined. It is then filled with milk in a similar manner as in case of water; the tem- perature of the milk should be slightlj' below GO" F., and is gradually brought up to this degree after the fiottle has been filled, proceeding in the same manner as before with water; the weight of the bottle and milk is then taken. The weights of water and of milk contained in the specific gravity bottle are found by subtracting the weight of the the empty bottle from the second and third weight, respec- tively, and the specific gravity of the milk then found b}' dividing the weight of the milk by that of the water. 1 88 Testing Milk and Its Products. Example: Weight of sp. gr. bottle + water. ..14-6. 0113 grams. Weight of sp. gr. bottle empty 46.94-23 Weight of water 99.9690 grams. Weight of sp. gr. bottle + milk 149.8718 grams. Weight of sp. gr. bottle empty 46.9423 Weight of milk 102.9285 grams. Sp.gr. of milk = -^-1=1^=1.0296 230. If a plain picnometer without a thermometer at- tached, is available, the method of procedure is similar to that described, witli the difference that the temperature of the water and of the milk must be brought to 60° F., before the picnometer is filled, or the picnometer filled with either liquid is placed in water in a small beaker, which is very slowly warmed to 00° P. and kept at this temperature for some time so as to allow the liquid in the picnometer to reach the temperature desired; the temperature of the water in the beaker is ascertained by means of an accurate chemical thermometer. The perforated stopper is then wiped off. the picnometer is taken out of the water, wiped and weighed. It is necessary to weigh very quickly if the room temper- ature is much above 60° F., as in such cases the expanding liquid will flow on to the balance pan, with a resultant loss in weight from evaporation. The weights of specific gravity bottle or picnometer, empty and filled with water, need only be determined a couple of times, and the averages of these weighings used in subsequent determinations. •^:51. b. Water. The milk is weighed into a perforated copper tube filled with prepared dry asbestos. The tubes are made from perforated sheet copper, with holes about Chemical A)ialysis of Milh and J Is Products. 189 .7 mm. in diameter and al)iiut .7 mm. apart; thej' are ilii mm. long, 20 mm. in diameter and closed at the bottom. The asbestos is prepared from clean fibrous asbestos, which is ignited in a mullle oven, treated with dilute IICl (1 ; o) and then with distilled water till all acid is washed out; it is then torn in loose layers and dried at a low temperature in an air bath; when dry it can be easily shredded in fine strings and is placed in a wide-mouth, glass-stoppered l.)ottle. About 2 grams of asbestos are placed in each tulie, pack- ing it rather loosely; the tube is then weighed, a small narrow beaker being inverted over it on the scale pan. 5 cc. of milk are then dropped on to the asbestos from a 5 cc. fixed pipette, the beaker again placed over the tube, and the weight of the 5 cc. of milk delivered + copper tube taken. The weight of the milk is obtained by ditlerence. The tubes are now placed in a steam oven and heated to 100" (J. until thej' no longer decrease in weight, which ordinarilj- will take about three hours. Place in dessiccator until cold, and weigh; the difference between the weight of the tube 4- milk and this last weight gives the water contained in the milk, which is then calculated in percent of the quantity of milk weighed out. Example. Weight of tube -I- breaker + milk 29.3004 grams. Weight of tube + beaker 2-t.l772 Milk weighed out 5.1232 grams. Weight of tube -f beaker + milk 29.3004 grams. Weight oftube + beaker + milk, dry. .24. 9257 Weight of water 4.3747 grams. -n J- r i ■ -11 4.3747X100 or o,^ Percent of water m milk ^= ^ 85.39 percent. 5.1232 ^ Note. The percent of total solids in milk is often given, instead of that of water; this may be readih' obtained by ipo Tcs/mn- Milk and lis Products.. subtracting the weight of the empty tulie from that of the tube filled witli millf solids, and finding the jjercent of the milk weighed out which this difference makes. In the above example, the weight of milk solids thus is 24.0257 — 24.1772 = .74S5 grams, and the percent, of total in the milk = 14.01 percent. '233. Alternate method. 5 cc of milk are measured out on a weighed Hat porcellain dish (50-110 mm. in iliameter; porcellaiu crucible covers will answer the purpose better than any other vessel on the market, provided the handle be broken off or ground off level on an emory wheel); this is weighed rapidly; two or three drops of 30 percent acetic acid are added, and the dish is dried in a steam oven at 1 00"" until no further loss in weight is obtained. After cooling in desiccator, the weight of the milk solids is obtained, and by calculation as before, the percent of water or total solids in the milk. 233. 0. Fat. The dried tubes from the water deter- mination are placed in (..laldwell extractors ami connected with weighed, numbered glass flasks (capacity. 2-1! oz); the extractors are attached to upright Ijiebig condensers and the tubes extracted with pure ether, free from water, alco- hol or acid, until all fat is dissolved; 4-5 hours' extraction is sufficient; in case of samples of skim milk it is well to continue the extraction for (i hours. The ether is then dis- tilled off and recoN'cred, and the flasks dried in a copper oven until constant weight; after cooling they are weighed, and the amount of fat (tontained in the quantity of milk originally weighed into the lubes is thus ascertained, and the percent, present in the milk calculated. Choiiical Auidysis of l\/ilk and lis Prodiicis. 19 I Example: Weight of flask -h fat 15.SO30 grams. Weight of flash 15.5171 Weight of fat 2S68 grams. Milk weighed out 5.1232 grams. I'er cent, of fat m milk = = .j.oS pereent. 5.1232 'i'M. Casein and albumen. The sum of these com- ponents is generally determined by the KJeldahl method,* 5 cc. of milli are measured carefully into a flat-bottom SOO cc. Jena flask, 20 cc. of concentrated sulfuric acid (C. P., sp. gr., l.S-t) are added, and ,7 gram of mercuric oxid (or its equivalent in metallic mercury); the mixture is then heated o\er direct flame until it is straw colored or perfectlj- white; a few crystals of potassiufn perfnanganate are now added till the color of the liquid remains green. All the nitrogen in the milk has now been converted into the form of am- monium sulfate. After cooling, 20(1 cc. of distilled water, free from ammonia, are added, 20 cc. of a solution of pot- assium sulfid (containing -iO grams sulfld per liter), and a fraction of a gram of powdered zink. A small ciuantity of semi-normal Hf'l solution, more than suflicientto neutralize the ammonia obtained in the oxidation of the milk, is now carefull}' measured out from a delicate burette (divided in ~ cc.) into an Erlenmeyer flask, and the flask connected with a distillation apparatus. At the other end, the Jena flask containing the water}' solution of the ammonium sul- fiate is connected, after adding 50 cc. of a concentrated soda solution (1 pound " pure potash " dissolved in 7)00 cc. of distilled water, and allowed to settle); the contents of the Jena flasks are now heated to boiling, and the distillation is -'■ l'""reteeniu8' ZeitHrfirifi, [i. '^-J, 360; U. S. Dejit. Agr,, Cbem, Piv,, biilf , 43, jcj2 Tcslino- Milk and Its Products. coatinued tor 40 minutes to an hour, until all the animonia hag been distilled over. The excess of acid in the Iilrlenmeyer receivinj^ flask is then accurately titrated back by means of a tenth-normal standard ammonia solution, usin^ a cochineal solution^ as an indicator. From the amount of acid used, the percent of nitrotfen, and from it. the percent of casein and albumen in the milk is obtained by multiplying by 6.25.^ The amount of nitrogen contained in the chemicals used is determined b_v blank experiments, and deducted from the nitrogen obtained as descritjed. Example: The weight of ~j cc. of milk Cas obtained in deter- mininff the water in milk, see a. i was ^j.UfJ'j grams. -5 cc, of standard HCl are added in the receiver, and 1..0.5 cc. of-— alkali 10 solution are used in titrating back the excess of acid. 1..!;.!; cc. of ^- alkali = 1 ^ .ol cc. V acid solution; the ammonia distilled 10. o 2 over therefore neutralized o — .ol^iAU cc. acid. By blank trials it was found that the reagents used furnish an equivalent of .02 cc. acid in the distillate: this quantity subtracted from the acid-equivalent of the nitrogen of the milk leaves 4.47 cc. 1 cc. semi-normal HCl. solution corresponds to 7 milligrams or .007 X grams of nitrogen; 4.47 cc. — HCl. therefore represents .03129 grams of nitrogen. This quantity of nitrogen was obtained !rom the .5.140.5 grams of milk measured out: the milk therefore con- tains ^^^^;^-^' — :- = .60S percent of nitrogen, and .608 X 6.25 .5. 146.0 = .3.80 percent of casein and albumen. 235. Casein and albumen may be determined separately by Van .-^lyke s method: 1 10 grams of milk are weighed out * Sutton, \'oli]mGtrio Afialysia, 4th edition, p. 31. t The factor G.;iO or ^;.::7 Ii more correct for the albarfleooids of ruHb, bat haa not yet b^en generally adopted. X Bulletin No. 43, p. 183, Chem. Division, U. S. Dept. of .Kgricultare. Cheniical Analysis of Milk and Its Products. 193 and diluted with about IM) cc. of water at 40-42' ('. 15 ee. of a 10 percent acetic acid solution are then added; the mixture is well stirred with a glass rod and the precipitate allowed to settle for 3-5 minutes. The whey is decanted through a filter and the precipitate washed two or three times with cold water. The nitrogen is determined in the filter paper and its contents by the Kjeldahl method; blank deterniinatitins with the regular nuantitics of chemicals and the lilter paper used are made, and the nitrogen found therein deducted. The percent of nitrogen obtained multi- plied by C.'_'5 gives the percent of casein in the millv. ■2;{(). Albitnicii is determined in the liltrate from the casein precipitate; the liltrate is placed on a watei' bath and heatiMJ to boiling tem|)erature ol' water ri.>r ten to tifteen minutes. The washed preciiiitate is then treated by the Kjeldahl metJKjd for the determination <;)f nitrogen; the amount of nitrogen multiplied b)' tb25 gives the amount of alluiiuen in tiio millv. The ditiercnce between tlje total nitrogenous com[)onents found by tlie l\'jeldahl mctliod. and the sum oi' tlie casein and the alliumen, as given abii\-e. is due to the [irt-sencc in milk of a third class of nitrogen compounds, cidled b_y various authors, globulin, lacto[iepton, nucleiu, etc. (] S). "i:} ( . e. Milk sugar is genei-ally determined fiy difterence, the sum ot fat, casein and alliumeu (total N x i'.-'5), and ash. being sulitracted from the total solids. It may lie de- termined directh' b}' means of a polariscopc, or grax'inictric- ally by Fehling's solution; only the former method, as wiu'ked out fiv A\'iley, * will l)e given here. The specific gravity of the milk is accurately determined, and the folloNving quantities of milk are measured out by •■ A^'riCLiltural Analysis, iii, p. -'',; Am. Clieiu. Jour., fi, p. 2sLi et s^ip 13 194 Tcs/iiig Mill- (lud lis Products. means of a Kill cr. pipette graduated to .2 cc. (or a 04 cc. pjipettc made esjii'cially foi- this purpose, with marks on the stem Ijetween (iS.T and (jl.li ee.), according to the specific gravities given: 1 .(IL'C, 04.!! cc; ].n28, 044 5 cc; 1 4):;0, (144) cc; 1.(132, G3.9 cc; ] 4)o4, G3.S cc; 1.030, 03.7 cc. These quan- tities refer to the Soleil-Ventzke Jialf-shadow polariscopes, re- quiring a normal weight of 20.0 IS grams of sugar. The milk is measured into a small flask graduated at 100 cc. and 102.0 cc; 30 cc of mercuric iodid solution (prepared from .33.2 grams potassium ioditl, 13.5 grams mercuric chlorid, 20 cc. glacial acetic acid and 040 cc water) are added; the flask is filled to 102. G cc mark with distilled water, the contents mixed, filtered through a dr}' filter, and when the filtrate is per-feetl}' clear, the solution is polarized in a 200 millimeter tube. The readings of the scale divided b3' 2 shows the per- cent of lactose (milk sugar) in the milk. Take five readings of two different portions of the filtrate, and average the results. 2;}S. f- Ash. About 20 cc of milk are measured into a flat Ijottom pjorcellain dish and weighed; about one-half of a cc. of 30 percent acetic acid is added, and the milk first drieil on water bath, and then ignited in a muffle oven at a low red heat. Direct heat should not be applied in deter- mining the ash of milk, since alkali chlorids are likelj' to be lost at the temperature to which milk solids have to be heated to ignite all organic carbon. Example. Weifjlit of porccllriin dish + milk 49.0',)07 grams. Weight of porccllain dish 28. 353s " WciRlit of milk 20.7300 grams. Weight of rjisli + milk, ;ifter ignilion..2S.5037 grams. Weiglit of rlish ' 28.::i53S Weight of milk ash 14y'J grams. T, , f , .1409x100 Pereent or asn= = . i J ijerccnt. 20.7300 Chcmicdl Aihtlysis a/ Milk and /Is Products. iq^ 2;}!). Acidity of milk. The acidity of milk is con- veniently determined by means of Farrini^ton's alkaline tablets (see p. It'.)), or by one-tenth normal soda solution. Tu the latter case, l!Occ. of milk are measured into a porcellain casserole; a few drops of an alcoholic phcnolphtalein solu- tion are added, and soda solution is dropped in slowly from a burette until the color of the milk remains unitorinly pinkish on agitation. ] cc. of '- alkali corresoonds to .0(l!> r ^ lo ' " grams lactic acid, or to .ti4.'i [)ercent, when I'd cc. of milk arc taken, (see p. US). Detection of Preteuvatives in IMilk. '24:0. a. Boracic acid (bonu-, borates, jiresi-rvallne, etc.) 100 cc of milk are made alkaline with a soila or potash solution, evaporated to dryness and incinerated. The ash is dissolved in water to which a little hydrochloric acid has been added, and the solution liltered. A strip of turmeric paper moistened with the filtrate will be colored reddish brown when dried at lOi)- C on a watch glass. If a little alcohol is poured over the ash to which cone, sulfuric acid has been added, and fire is set to the alcohol after a little while, it will burn with a yellowish green tint, especially noticeable if the ash is stirred with a glass rod and when the ttaine is about to go out. 341. The following modification of the lirst test given is said to prove the presence of onl}- a thousandth of a gram of boras in a drop of milk* (.13 percent.): Place in a porcellain dish one drop of milk with two drops of strong hydrochloric acid and two drops of saturated turmeric tinct- ure; dry this on the water bath, cool and add a drop of am- * N. .J. Dairy tMjiniuiasioner, report l.'^'.iG, ]i. 37. 196 Testing Milk and Its Products. monia Ij)- means of a glass roil. A slaty blue color, chang- ing to green, is proilueed if borax is present/' *24'2. b. Bi=Carbonate of soda. 100 cc. of milk to which a few drops of alcohol are added, are evaporated and carefully incinerated; the proportion of carbonic acid in the ash, as compared with that of milk of known puritj', is de- termined. If an apparatus for the determination of carbonic acid is availalile, like the Scheibler apparatus, etc., the per- cent, of carbonic acid per gram of ash (and quart of milk) can be easily determined. Normal milk ash contains onlj- a small amount of carlionic acid (less than 2 per cent), pre- sumably formed from the citric acid of the milk in the pro- cess of incineration. The following quantitative test is easily made: To 10 cc. of milk add 10 cc. of alcohol and a little of a one-percent rosolic acid solution. Pure milk will give a brownish yellow color: milk to which soda has been added, a rose red color. X control experiment with milk of known purity should Ije made. '2-1-3. e. Fluorids. 100 cc. of milk are e\'aporated in a platinum or lead crucible, and incinerated; the ash is made strongh' acid with cone, sulfuric acid. If lluorids are present, hydrolluoric acid will tic generated on gentle heat- ing, and will be apparent from its etching a watch glass placed over the crucible. '2':l:-l. d. Salicylic acid (salicyhites, etc.). 2f) cc. of milk are acidulated with sulfurii.- acid and shaken with ether: the ether solution is evaporated, and the residue treated with alcohol and a little iron chlorid solution; a deep \'iolet color will be obtained in the presence of salicj'lic acid. « See also i:i2, i:;7 Chemical A)ialysis of Milk and Its Products. 197 "ilo. e. Formaline (a forU'-percent solution oi funnal- dehijde in water). A solution of diphenj'lamin is made with water and just enough sulfuric acid to secure a proper sol- vent effect. The milk to be tested, or better, the distillate therefrom, is added to this solution and boiled. IE formaline be present, a white flocculent precipitate is formed; if the acid used contained nitrates, a green precipitate will be formed.* B. — Ski.m-'milk, Butter-milk, Whey. 24G. The analj'sis of these products is conducted in the same manner as in case of whole milk, and the same con- stituents are determined, when a complete anal3'sis is wanted. As they generally contain onl}- small quantities of solids, and especially of fat, it is well to weigh out a larger quan- tity than in case of whole milk; if possible, toward 10 grams. The acidity of some milk and butter milk must lie neutral- ized with sodium carlionate prcs'ious to the dr_ying and ex- traction, as lactic acid is soluble in ether and would thus tend to increase the etiier-extract (fat) if not combined with an alkali previous to the extraction. ( '. — Butter. 218. Sampling. A four to eight-ounce sami)le of but- ter is melted in a tiglitlj' closed fruit jar, shaken vigorously and cooled until the butter is hardened, the jar lieing shaken at short intervals during the cooling so as to kee[) the water of the Lmtter evenly distributed in the mass. 24y. a. Determination of water. Small i)ieces of butter (about i' grams in all) are taken from the sample by means of a steel spatula, and placed in glass tubes, sevcn- '■ See also Clieiu, New.*, IMi.;, Nn. 71; JUIchzeitimg, ls;)0, J91; ISOT, ipS Tcs/iiiq- I\filk and Its Products. eightlis of an inch in diameter and two and a half inches long, closed at the bottom by a layer of stringy asbestos, and filled two-thirds full of asbestos prepared as for milk analysis (L'ol). The tuljes arc dried at 100"" C. in the water oven, until no further loss in weight takes place, and are then cooled and weighed. The percent of water is deter- mined as in case of milk analj'ses. 2oO. Ij. Fat. The tuljcs are placed in Caldwell's ex- tractors and extracted for four hours with anhydrous ether; the ether is then distilled off, and the flasks dried in the steam bath and weighed, the increase in weight being the fat in the samples of Ijutter weighed out. '251. c. Casein. 10 grams of l^utter are weighed into a small beaker provided with a lip, and treated twice with about 50 cc. of gasoline each time; the solution is filtered off, and the residue transferred to a filter and dried; its ni- trogen content is then determined by the Kjeldahl method (234). The nitrogen in the filter and chemicals used is deter- mined liy blank trials and deducted. The nitrogen multi- plied by 0.2.5 gives the casein in the liutter. 252. d. Ash. 1. 10 grams of butter are weighed into a porcellain dish and treated twice with gasoline, as in pre- ceding determination, the solution is filtered through an ash-free (quantitative) filter, and the filter when dry, is transferred to the dish. The dish is heated in an air-bath for half an hour and then placed in a muflle oven, where the contents are ))urnt to a light greyish ash; the dish is now cooled in a desiccator and weighed. The ditfercnce between this weight and tliat of the empty dish gives the amount of ash in the butter weighed out. Chcniici-il ^[iihIys/s ''/",]////• mid /Is I'rodiicls. iqg 253. -■ Aliout 1' grams of Ixitter are weighril into a small porcellain dish, half tilled with stringy asliestos; the dish is dried for an hour in the water oven, and the lat then set fire to with a match, the asbestos filler serving as a wick. When the flame has gone out, the dish is placed in a mullle oven, and the residue burnt to a greyish ash. After cool- ing, the dish is weighed, and the percent, of ash in the butter calculated as under method a. 254:. Complete analysis of butter in the same sample. About 2 grams of the flutter are weighed into a platinum gooch, half lilled with strings asbestos, and dried in water oven at 100-' ('. to constant weight, cooled and weighed. The difference gives tvalei- in the sample. The gooch is then treated repeatedly with small portions of gasoline, suction lieing applied, and again dried in the water oven, cooled, and weighed; the fat in the sample is olitained from the ditterence between this and the preceding weight. The gooch is then carefully heated over direct flame until a light greyish ash is obtained; this operation is preferably done in a muffle oven to avoid possible loss of alkali chlo- rids. The loss in weight gives the casein in the sample weighed out, and the increase in the weight of the gooch over that of the empty gooch with asliestos gives the ash (mainly salt) of the butter. The salt in the asli may be dis- solved out by hot water, acidulated with a drop or two of nitric acid, and the chlorin content of the solution deter- mined by means of a standard silver nitrate solution using potassium chromate as an indicator. 200 Testing Milk and Its Prodiicls. Dp:tection of Artificial Butter. 255. Determination of the specific gravity of the filtered butter fat serves as a good preliminary test. A number of practical methods for the detection of artificial butter have been proposed, but they are either worthless, in case of sam- ples containing a considerable proportion of natural butter, or give satisfactory results only in the hands of ex|jerts. The Reichert-Wollny method given in detail below is the standard method the world over, and the results obtained by it are accepted in the courts. 250. Filtering the butter fat. The butter to be ex- amined is placed in a small narrow lieaker and kept at HO' ('. for aljout two hours. The clear supernatant fat is then li Itered through absorl)ent cotton into a 200 cc. Erlenmeyer llask. taking care that none of the milkj- lower portion oi' the contents of the beaker be poured on the filter. In sampling the butter fat, it is poured back and forth repeatedly tVom a small warm beaker into the fiask, and the quantity wanted is then drawn off with a warm pipette. 257. Specific gravity. This is generally determined at 100^ C. The method of procedure is similar to that de- scribed under milk (230). The picnometer (capacity aljout 25 cc.) filled with dry filtered butter fat. free from air l)ubbles; the fat is heated for :!0 minutes in a beaker, the water in which is kept boiling. On cooling, the weight of picnometer and fat is obtained, and liy calculation as usual, the specific gravity of the I'al. The specific gr;ivity of pure natural butter fat at 100^ (J. ranges between .si.lfjO and .8085, while artificial butter fat (i. e. I'at from other sources than cows' milk) has a specific gravity at 100'" C. of below .8010, and generally aliout .85. Chemical Analxsis of Milk and Its Products. 20I •25S. Reichert'WolIny m^W^oA (Yolatile Adds) . .l.Tocc. of tat are measured into a stroDg 250 cc. weiglied saponi- fication flask, by means of a pipette marked to delivcr- this amount, and the flask when cool, is weighed again. 10 cc. of 95 percent, alcohol and 2 cc. of a concentrated soda solution (1:1) are then added to the flask which is securelj- stoppered with a cork stopper tied down witli a piece of twine. The flask is heated for an hour on the water bath, being gentl3' rotated from time to time in order to facilitate the saponification. The flask is then uncorked, the alcohol evajjoratcd slow!}' and the heating continued until the last traces of alcohol are gone. 1()(( cc. of recently-ljoiled, distilled water are now added, and the llask heated on the water bath until the soap formed is completely dissolved. When cooled to about 70' C, 40 cc, of dilute sulfuric acid (25 cc. cone. 11, SOj per liter) are added to the soap solution to decompose the soap into free fatty acids and glycerol. The flask is restoppered and heated until the insolulile fatt)' acids separated out form a clear oilj' layer on the surface of the acid solution of the flask. After cooling to room temperature, a few pieces of pumice stone (prepared I13' throwing the pieces at a white heat into distilled water and keeping them under water until used) are added, the flask connected with a glass condenser, heated slowly till boiling liegins, and the contents then dis- tilled at such a rate as will firing 110 cc. of the distillate over in as nearly thirty minutes as possible. The distillate is mixed thoroughly and filtered through a dr}' filter; 100 cc. of the filtrate are poured into a 250 cc. beaker and titrated with a deci-normal barium hydrate solu- tion, hair a cubic centimeter of pheuolphtaleia solution being used as an indicator. A lilank test is made in the 202 Tcstiii!^- Milk (Did lis Protliifts. same mruiner as dcscribeil, and the alkali solaticjn required dedueted Iroin tlie results obtained with the samples aualj'zed. The nurnlier oi" culiic eentimeters of barium hydrate solution used is inereased by one-tenth, and the so- called Reichert number thus obtained. The li.eichert number for pure butter fat will ordinarily come above 24 cc; butter fat from strippers will have a low Ileichert number. Pure oleomargarine will have a Reichert number of 1-2 cc. ; and mixtures of artificial and natural butters will show intermediate numljers. D. ClIEKSE. For method of sampling, see p. 7s. 2.>'t. a. Water. Five grams of cheese cut into verj- thin slices are weighed into a small porcellain dish filled about one-third full with freshly-ignited stringy asbestos; the dish is placed in a water oven and heated for ten hours. The loss in weight is taken to represent water. '2(>0. b. Pat. About 5 grams of cheese are ground finely in a small porcellain mortar with about twice its weight of anhydrous copper sulfate, until the mixture is of a uniform, light blue color and the cheese evenly distributed through- out the mass. The mixture is transferred to a glass tube, of the kind used in butter analysis (210), only a larger size; a little copper sulfate is placed at the bottom of the tube, then the mi;tture containing the cheese, and on top of it, a little extracted absorbent cotton or ignited, stringy asbestos; the tube is placed in an extraction apparatus and extracted with anhydrous ether for 15 hours. The ether is then dis- tilleil off, the flasks dried in a water oven at 100' C, to con- stant weight, cooled and weighed. The method is apt to Cht'iiiiciil ^[ualvs/i of Milk ciiid lis Prothirfs. 203 give tO(j low rcsultw ami, therefore, not to lie preferred to the Bal)Coek test for cheese ('.tl), 261. e. Casein (total nitrogen ';■' C.25). Aliout 2 grams of cheese are weighed out on a watch glass and transierred to a Jena nitrogen flask, and the nitrogen in tlie sample de- termined according to the Kjeldahl metliod; the percentage ot nitrogen multiplied by G.L'5 gives the total nitrogenous components of the cheese. '2(j'2. d. Ash. The residue from the water determina- tion is taken for the ash; it is preferably set fire to, in the same manner as explained under determination of ash in butter (25.'1), 'lefore it is placed in the muille oven and in- cinerated. The increase in the weight above that of the empt}' disli + asbestos, gives the amount of ash in the sam- ple weighed out. "iGo. e. Other constituents. The sum of the percent- ages of water, fat, casein and ash, sul3tra(.'ted from lOn will give the percent, of other constituents, organic acids, milk sugar, etc., in the cheese. Detection of Oleomargarine Chee.se (-'Filled" Cheese) 20 grams of cheese are extracted with other in a Caldwell extractor or a paper extraction cartridge; the ether is dis- tilled off, and the fat dried in the water oven until there is no further loss in weight. 5.75 cc. of the clear fat are then measured into a 250 cc. saponification flask and treated ac- cording to the Reichert-Wollny method, as already ex- plained under Detection of Artificial Butter (255). APPENDIX. Table I. Composition of milk and its products. No. of 11 Water Fat. C'ayeiii Milk Ash. Authority. llb'men ]'T. Ct ])r rt. lir. il J.r. et l.r. ct Cows' milk 793 87.17 3 69 3.55 4 . S,s .71 Koenig.5 • > >i S7 75 3.40 3.75 3 . 50 3.10 4.60 5 . Kl . 7 5 .65 Fleisclimann. ., 900' 87.40 Van Slvke. .1 .1 2173 8ii.4S 4 . 20 3.51- 1: .71 Holland. 11 Condensed milk ( sugar added 1 1)4 25.61 10.35 11.711 50.06 2.19 " Butter 302 10 13.59 84.39 12.93 84.53 .74 .7(1 .61 .68 .66 1.25 " " sweet cream " " sour cream.. 11 13.08^4.26 .81 .66 1 . 1 9 " unsalted 7S 13,73 84. S2 1 . 36 .09 " World'sFair 350 11.57,S4.7( .95 2.7^ Farrington. " American i premium... 9 10.23'85.74 . tic :-; , 05 Morrow. Cheese, crcim.. 27 36. ",3 4 0.71 1 8 . S4 1 . 07 3.1( Koenig.S " full cream. 143 .38.60 30.27 25.:;5 1 1.4:- 4.9; " " Cheddar, 36.84,33.8:; ■"'3.7-' 5.61 : \'an Slvke. " Cheddar, cured liT 34.3S'32.71 26.:i8 2.95| 3.58 Drew. " Worl'sFair mammoth 1 32.06 34.13 128.(10 ! 5.51 Shutt. " half skim. 21 39. 7923. 9-: 29.67 1 1.71 4.7: Koenig.S " skim 41 46.00 11.67 34.06 3.47 4.87 ' ' " centrifuga 50 5 1 2 43.1 5.2 Storch. * 42 analyses, f S aDalyscs. :;: 13. On iierueDt alliunieu. ^ Mostly liuropean samples. ;, Massachusetts samples. 206 Tcslin>'- Milk and lis Products. Table II Milk standards. Maine New Hampshire A'erm on t in May and June Massaclnisetts in May and June sliimmcd milk Rliode Island New York Newjersey Pennsy 1 vania *' District of Columbia Georgia .South Carolina Ohio Michigan Wisconsin Minnesota!' Iowa Oregoni Washington City of Chicago " St. Louis " " cream, " Omaha " " cream. " Denver lingland.... ("jcrmanv S. Sol Mis. VIS) l.'i.O VI -, 113.0 l.'i.O 12.0 12.0 12.0 12.0 12. ,5 12.0 12.0 11..":; 12.5 12.5 l.'i.O 12.5 12.0 12.0 22.0 12.0 12.0 [11.5] Soli'ln rjo(. fat. rurcenl. 3.0 [3.25] .'; [3.7] 3.0 3.0 o r 3.0 3.0 3.0 3.0 3.5 3.0 3.2 3.0 3.0 2.8 12.0 3.0 10.0 I'lircent. [0.0] 0.25 [■,).0] 9.3 [0.5] [0.0] [9.5] [9.0] [S.5] [,S.5] [9,5] [9., 5] [9.5] [9.2 [9.0] [9.2] [10.0] [9.0] Law or OrrJirjaDC'i:^ of 1893,255 1 883 1888,108 1886,318 1885,252 1893.338 1882i82 1885,100 1889,86 1889,219 1889,425 1889,247 1892,. 50 1893 1892 1887 1893 1893 « Specific gravity, 1.iil;'.i — 1.0:'.:i at Wp V. \ Oheese. 40 percent, fat in soliils. X Jiutter, 1 1 percent, water. 'i Specilic gravity, l.ll'J'J at Vp C. A-ppciidix Table III. Quevenne lactometer degrees correspond- ing to N. Y. Board of Health lactometer degrees. (See p, s;:;). Bd.ofUeiiltli (^levpiiDC r.d.of HeaUli *"^iieveune Bd. of Health QueveDlie decrees. scale. degrees. scale. degrees. scale. 110 17.4 81 23.5 101 29 . 3 01 17.7 82 23.8 102 2; 1 . 6 6'J 18.0 83 24.1 103 29 . 9 03 18.:! 84 24.4 104 30 , 2 04 18.i; 85 20 . 6 105 30.5 65 IS. 8 80 24 . 9 100 30 . 7 00 19.1 87 25 . 2 107 31.0 07 19.4 88 25.5 108 31.3 68 19.7 89 25.8 109 31.0 69 'JO . 90 26.1 110 31.9 70 29.3 91 26.4 111 32.2 71 2(.) . 6 92 26.7 112 32.5 72 20.9 93 27.0 113 32.8 / O 21.2 94 27.3 114 33.1 74 21.5 95 27.6 115 33.4 75 21.7 90 27.8 116 33 . 76 22.0 97 28.1 117 33.9 77 22.3 98 28.4 118 34.2 78 22.6 99 28.7 119 34.5 79 22.9 100 29 120 34.8 80 23.2 2oS Testintj; Milk and Us Products. Table IV. Correction table for specific gravity of milk. Temperature of rnilk (in d igrees F jljrenhe t). as — ~ z 51 52 53 54 ■JO 56 57 58 59 60 20 19.3 19.4 19.4 19.5 19.6 19.7 19.8 19.9 1 9 . 9 20 . 21 20.3 20.3 20 . 4 20.5 20.6 20 7 20 . 8 20.9 20 . 9 21.0 22 21.3 21.3 21.4 21.5 21.6 21.7 21.8 21.9 21.9 22 23 22.3 22.3 22.4 22.5 22.6 22.7 22.8 22.8 22.9 23.0 24 23.3 23 . 3 23.4 23 . 5 23.6 23 . 6 23.7 23.8 23 . 9 24 . 25 24.2 -!4.3 24.4 24.5 24 , 6 24.0 24.7 24.8 24.9 25 . 26 25. 2i 25.2 25 . 3 25.4 25 . 5 25 . 6 25.7 25.8 25 . 9 26.0 27 21;. 1! 2f;.2 26.3 26 . 4 26.5 26 . 26.7 26 . 8 26.9 27.0 2S 27.1' 27.2 2/ . 3 27.4 27 .5 27.1; 27.7 27 8 27 . 9 28 . 211 28.1' 28.2 28.3 28.4 28.5 28 . 6 ''L'6 . 7 28 '. s 28 . 9 29 30 20.1 20.1 29 2 29.3 29.4 29 6 29 . 7 29 s 29 . 9 .30.0 31 30.0 30.1 30.2 30 . 3 30.4 .30.5 30,6 30 '. 8 30.9 31.0 32 31.0 31.1 :-; 1 . 2 31.:; 31 .4 .31 .5 31 .6 31.7 3 1 . 9 32.0 33 31.11 32.0 3::.l i J 2 , .' ! 32.4 ■32. -J 32 6 ?>■' ~ 92.9 33.0 34 32.9 33 . 33.1 33 . 2 .'i3 . 3 33 5 3.3.6 ■'")>') . / 33 . 9 .34.0 35 33.8 33.9 34 . 34.2 34.3 34 ^5 34.6 .34.7 34.9 35.0 61 iVi 03 04 65 66 67 68 69 70 20 20.1 20.2 20.1; 20 . 3 20 . 4 20 . 5 20 . 6 20 7 20.9 21.0 21 21.1 21 V 21 .3 21 .4 21.5 21 .6 21 .7 21.8 22 . 23.1 22 22. r 22 •1 22.3' 31;. 4 '' ■' .5 22 . 6 2'* 7 22 . 8 23 . (1 23.1 '^3 23. ]i 23 •i 2'"J -i 23.4 23 5 2.'. 'i 33 . ' 23 8 24.0 24.1 24 24.1 24 24^3 24.4 24.5 21.6 24 . 7 34^9 25 . 25 . 1 25 25 . 1 25 'J 25 . 3 25 . 4 2') . ■) 25 . 6 25 . 7 2) . 9 26.0 26 . 1 26 26.1 20 Q 20.3! 211.5 36 'i 26 . 7 2''. 8 27 . 27.1 2~ '^ 27 27.1 27 37 . I 27.5 27 .0 '27 7 27.8 28 .0 28.1 2 s . 2 2-S 28. 1 28 3 28.4 28 . 5 28 . 6 28.7 38 , S 2ll.( 30.1 29 . 2 21) 29.1 29 29.4 29 . 5 29.6 29.7 29,9 30.1 30.2 30.3 30 30.1 30 311. 1 3.0.5 311.7 3.(1,8 30.1 3,1.1 3 1 . 2 3,1.3 31 31.2 ;;i 3.1 .4 3 1 . 5 31 .7 31 .s 3 1 . 1 32 . 1 32.1 32 . 4 • •o 32 2 '>■) ".» r '."■) (' . 1 .> - ; \ -J \ .' ', 3 ( 33 . 3 33 3 3,3 . 4 33, :;:; " 3:; 3.3. ,8 WW . 1 :! 1 . 1 .31.2 :; 4 . 3 34.5 34 31. -J :;i 3 1.5 ;;i.c 31.8 w\.\ 35 . 1 35 . '_ 35.: 35.5 35 :jo . ii 35 ■'• 3.) .5 -■ ! 5 . ' i 3.5. S 3.1.9 3,6.1 36.2 36.4 36 . 5 ll[KKrrnnNs. — liriiiK Hi'- l<_Mji]icr;ilLireof llic luilk to witliiu lO"^ from C,iP p. Tyk<: t,ln'. reinlirij^' of tlio l;H:t,oiiii.-i 3 .3 .3 . ;; ;".; .3 - .3 9 8 .2 2 2 .3 3 ^3 .3 .3 , .3 .3 8 ; 2 '~1 ri .2 '2 .2 2 2 '2 2 9 .2 i 6 .1 Q .2 2 • 2 .2 2 . 2 6 5 >■} '2 9 ' 2 . 2 2 2 6 4 J J .1 A .1 '.] .1 .1 J .1 .1 J 4 3 .1 .1 .1 .1 .1 .1 .1 .1 . 1 .1 .1 .1 2 .1 .1 . 1 .1 .1 .1 .1 .1 .1 .1 .1 .1 1 3 . 00 3.06 3.10 3 . 1 5 3 . 2i 1 3 . 25 3.30 3.35 3.40 3.45 3.50 3.55 1 r.. 2 B Appendix. 213 Table YI . Lbs. of fat ill 1 to 10,000 lbs. of milk [Continued] 6 H 3 . 00 ;.65 5.70 >.75 J. 80 3. 85 3 .903. 95 4 . 00 4 . 05 4.104.15 t Milk Mlik lbs. 111.'-. 10, 000 360 365 370 375 380 385 390 395 400 405 410 415 10, 000 9, 000 324 329 333 338 342 347 351 356 360 366 369 374 9,000 S, OOU 288 292 296 300 304 308 312 316 320 32'1 328 332 8,000 7, 000 252 256 259 263 266 270 273 277 280 284 2s7 291 7, 000 6,000 216 219 222 225 228, 231 234 237 240 243 246 249 6, 000 5, 000 ISO 183, 185 188 190 193 195 198 200 203 205 208 5,000 4, 000 144 146 148 150 152 154 156 158, 160 162 164 Kill 4,000 r,, 000 108 110 111 113 114 116 117 119 120 122 123 125 3,000 2,000 72.0 73.0 74.0 75.0 76.077.0 78.079.080.081.0 82 . 83 . 2, 000 1,000 30.0 36.5 37.0 37.5 38 . 38 . 5 39 . 39 . 5 40 . 40 . 5 41 . 41 . 5 1 , 000 900 .32.4 32 9 3:! . 3 33.8 34 . 2 34 . 7 35 . 1 35 . 6 36 . 36 . 5 36 . 9 37 . 4 900 SCO 28.8 29.2 29.6 30.0 30.430.8 31 .231. 6'32 . 32 . 4 32 . 8 33 . 2 80O 700 25.2 25.6 25.9 26.3 26.6 27.0 27. 327. 7,28. 0|28. 4128.7 29.1 700 600 21. (! 21.9 22 2 22.5 22.8 23.1 23 4:23. 7:24. 024. 324.624. 9 60O 500 18.0 18.3 18'5 18.8 19.019.3 19.519.8 20.0 20.3 20.5 20.8' 500 400 14.4 14.6 14.8 15.0 15.2 15.4 15.6 15.8|16.(!l6.2!l6.4 16 6 400 :;00 10.8 11.0 11.1 11.3 11.441.6 11.711.912.012.242.312.5 300 200 7 2 7 . 3 7.4 7.5 7.6 7.7 7.8 7.9i 8.0! S.l: 8.2 8.3 200 100 3 . 3.7 ■"' . ( 3.8 3.8 3.9 3.9 4.0; 4.0' 4,1 4.1 4.2 100 90 3.2 3 3 3.4 3.4 3.5 3.5' 3.6 3.6 3.7' 3.7 3.7 90 80 2.9 2 . 9 3^0 3.0 .3.0 3.1 3.1 3.2 3.2 3.2 3.3 3.3 80 70 2.5 2.6 2.6 2.0 2.7 2.7 2.7 2.8 2.8 2.8 2.9 2.9 70 00 '» '") 2 2 2 2 2.3 2 3 2 3 2^3 2.4 2.4 2.4 2'5 2.5 60 50 1.8 1^8 1^9 1.9 1^9 1.9 2.0 2.0 2.0 2.0 2.1 2,1 50 40 1.4 1.5 1.5 1.5 1.5 1,5 1,6 1.6 1.6 1.6 1.6 1.7 40 30 1.1 1.1 1.1 1.1 1.1 1.2 1.2 1.2 1.2 1.2 1.2 1.2 30 20 . 7 . i . / .8 .8 . ^ .8 .8 .8 .8 .8 .8 20 10 .4 A .4 .4 ^4 '.4 .4 .4 .4 .4 .4 .4 10 9 . 3 3 3 0; 3 .4 .4' .4 .4 4' .4 9 s ,3 •}, 3 3 3 .3 .3 .3 .3 .3 .3 8 ; ;:^ ^3 .3 ^3 ^3 .3 .3 .3 .3 .3 .3 7 G '1 9 (? 5 2 9 -") 2 '"^ '"• •'> 7} 4 .1 '\ J 2 <■") 2 2 " 22 2 4 ;; .1 .1 .1 .1 ''\ .1 '1 '1 '1 '1 A '.\' 3 .1 .1 .1 .1 .1, .1 .1 .1 .1 .1 .1 .1! 2 1 1 H H" 3.65 3.7C 3.76 3.80 3.85 1 ; , 3.903.954.004.064.104.15 f 214 Tcstino- Milk and lis Products. Table VI. Lbs. of fat in ] to 10,000 11)S. of milk (Continued) i . 204 . 25,4 . 30 4 . 35 4 . 40 4 . 45 ,4 . 50 4 . 55,4 . GO 4 . G5 Milk lbs. 10, 9, 000 000' 000, ooo' 420, 425, 378' 3S3' 33G! 340: 294 298 7 6, 000:l 252 255' 5, 4 3, 2, 1, 430 387 344 301 435 392 348 305 ,000 ,000 210 213 IGS' 170 OOOii 12G 128 000 84.0 85.0 000 42.0'42.5 258^ 261 218 174 215 172 129 131 86 . 87 . 43 .043.5 440 396 352 308 264 220 170 4.7014.75 445: 401 35G| 312 267J 2231 178' 450, 455: 460i 405| 410! 414' 3G0| 364 308 315 270 225 900 37 . 800'!33. 700|,29. 600l'25, 500,2!, 400 16 300 12 200,1 8 100 4 8 38.3 0i34.0 4 29.8, 2'25.5 0|21.3| 8 17.0 6I12.8' 4 8.5 2 4.3 38.7 34.4 1 132 134] 88.089.0 44.044.5 319 322 273 276 228| 230 ISO' 1821 184 135, 137| 138' 90.0,91.0,92.0 45.0'45.5 46.0 465 419' 372| 326' 2791 233| 186' 140 93.0 46.5 470, 475i 423' 428'' 3761 380:1 329 333 2821 285'! 235, 238, 188' 190 141 ! 143' 94.0 95.0 47.0'47.5 Milk lbs. 10, 000 9, 000 8, 000 7, 000 6, 000 5, 000 4,000 3, 000 2, 000 1, 000 90 80 70, 60, 50! I 40, 30!' 20,1 10 I 9,1 8 7 6: 5' 4, 2I 1 3.8 3.4 ■ 4 3.4 3.01 2.6' 2.li 1.7| I.3I .9j .4! 30 25 21 17 . 2! 12.9! 8.6| 4.3, 3.9 3.4' 3.0| 2.6 2.2| !| .4 39 . 2 39 . 6 40 . 1 40 . 5'41 . 41 . 4 41 . 9 42 . 3;42 . 8 34.8|35.2|35.6!l36.0|36.4l36.8'37.2'37.6'38.0l,' 30. 5,30. 8:31. 2 131.5,31. 9,32. 2:32. 6 32. 9j33. 3 26 . l|20 . 4|26 . 7 '27 . 0'27 . 3 27 . 6!27 . 9!28 . 2'28 . 5 21. 8122. 0J22. 3, 22. 5122.8123.0,23.3,23. 5123. 8 17. 4 17. 6ll7. 8' 18. 18. 2' 18. 4' 18. 6 18. 8 19.0 13.1 13.2!13.4 13.5 13.7ll3.844.0 14. 114.3 8.7 8.8! 8.9|| 9.0, 9.1, 9.2 9.3 9.4, 9.5, 4.4 4.4 4.5 4.5' 4.6' 4.6 4.7 4.7 4.8' .4, .3' .3 .3, .2! .2 .1 .1 3.9 3.5 3 . 0, 2.6, 2.2I 1.7 i.s! .91 .4 .4 .3 .3 4.0: 3.6'| 3.1i, 2.7 2.2'l 1.8,, 1.3 ■9|i .4', .4;; .4 .3 4.1; 3.6l 3.2i 2.7 2.31 4.1! 4.1 3.g! 3.7I 3.71 3.2; 3.2i 3.3, 2 S' 4.2 4.2' 3.8 3.3, .1 .1 .11' .1 4 . 20 4 . 25 4 . 30,4 . 35,4 . 40,4 . 45 ,4 . 50 2.7 2.3| 1.8 I.4I ■9| .5, :i .31 .3 '.•2\ .1 .it 2.8 2.4I 1.9, 1.9, 1.4' 1.4' .9 9', .5 .5 4.3 3.8 3.3, 2.9 2.4 1.9 1.4 1.0 .4; .3i .3, .2I .3! .1 .1 4.55 4.60 4.65,4.70 •4'! .4 .3 .3, .2, .2 .1 .1, .] .1|! 4.75,1 900 800 700 600 500 400 300 200 100 90 80 70 GO 50 40 30 20 10 9 8 7 6 5 4 3 2 1 Appendix. 215 Table TI. Lbs. of fat in 1 to tO,000 lbs. of milk ( Continued] Test. 4.80 4.85 4.90 4.95 '', . 00 5.05 5.10 5.15 5 . 20 5 . 25 5 . 30 5 . 35 H p Milk Milk lbs. ! lbs. 10, 000, 480 4S5 490 495 500 505 510 515 520, 525| 530 535 10,000 9, 000' 432 J 37 441 441; 450 455 459 464 468 473 477' 482 9,000 8,000 384 3881 392 396 400 404 408 412 416 420 422! -±28' 8,000 7, 000 336 340 343 347 350 354 357 361 364, 368 371 1 375,, 7, 000 6, OOq! 2881 29 ll 294 297 300 303 306 309 312' 315' 318 3211 6,000 5, 000 240 243| 245 248 250 243 255 258 260, 263 265 26811 5,000 4, 000 192 194' 196 19H 200 202 204 206 208 210 212' 214 4,000 3,000 144 1461 147 149 150 152 153 155 156 1581 159' 161r 3, 000 2, OOC 96.0 97.0,98.0 99.0 100 101 102 103 104| 105; 106, 107 2, 000 1, 000 48.0 48.549.0 49.5 50.0 50.5 51.0 51.5 52 . 52 . 5 53 . 53 . 5 1,000 900 43.2 43.744.) 44.6 45.0 45.5 45.7 46.4 46 . S 47 . 3 47 . 7 48 . 2 900 800 38.4 38 . 8 39 . 2 39 . 6 40.0 40.4 40.8 41.2 41 .642. 0'42 .442,8 800 700 33.6 34.034.3 34.7 35.0 35.4 35.7 36.1 36 4 36.8,37.1 37.5 700 600 28.8 29.129.4 29.7 30.0 .30.3 30.6 30.9 31.231.531.832.1 600 500 24.0 21.324.5 24.8 25 25.3 25 . 5 25 . 8 26.0 26.3 26.5 26.8 50(1 400 19.2 19.4,19.6 19.8 20.0 20.2 20.4 20 . 6 20.8 21.0 21.2 21.4 400 300 14.4 14.0 14.7 14.9 15.0 15.2 15.3 15.5 15.6 15.8 15.9 16.1 300 200 9 6 9.7| 9.8 9.9 10.0 10.1 10.2 10.3 10.410.5|10.610.7 200 100 4.8 4.9 4.9 5.0 5.0 5.1 5.1 5.2 5.2 5.3 5.3 5.4 100 90 4.3, 4.4 4.4 4.5 4.5 4.5 4.0 4.6 4.7 4.7, 4.8 4.8 90 80 3.8 3.9 3.9 4.0 4.0 4.0 4.1 4.1 4.2 4.2 4.2 4.3 80 70 3.4| 3.4 3.4 3.5 3.5 3.5 3.6 3 . 6 3.6^ 3.7' 3.7 3.7 70 60 2.9, 2.9 2.9 3.0 3.0 3.0 3.1, 3.1 3.1| 3.2 3.2 3.2 60 50 2.4I 2.4 2.5 2.0 2.5 2.5 2.6 2.6 2.6' 2.6' 2.7' 2.7 50 40 1.9| 1.9 2.0 2.0 2.0 2.0 2.O1 2.1 2.11 2.1 2.1 2.1 40 30 1.4, 1.5 1.5 1.5 1.5 1.5 1.5, 1.5 1.6 1.6 1.6 1.6 30 20 1.0 1.0 1.0 1.0 1.0 1.0 1.0 l.Q 1.0' 1.1 1.1 1 1 20 10 .5i .5 .5 .5 .5 .5 ■ 5 .5 .5, .5, .5 .5 10 9 .4 .4 .4 .4 .5 .5 .5 .5 .5 .5 .51 .5 9 S .4 .4 .4 .4 .4 .1 .4 .4 A .4 .4 .4 s _ 0! .0 . '-> 3 . 3 .4 .4 .4 .4 .4' .4 .4 .4 7 6 .3 .3 . -J ;-5 . . 3 . .3 .3 .3 .3 6 5 .2, . '2 • > . , r> . 'Z . 3 .3 .3 .3 .3 5 4 yl 2 9 2 2 . "2 .-) 2 2 4 3 !l| A .1 .1 '"2 ' 2, 2 2 2 3 2 .1 .1 .1 .1 .] '.\ .1 .1 A A A A 2 1 .1 5.00 .1 5.05 .1 5.10 .1 5 . 15 A A A A 1 1 4.9f 4.95 1 H 4.80 ! 4.85 5.20'5.25 5.3( 1 H 2l6 Tcstino- Milk and Its Products. Table VII. Amount due for butter fat, in dollars and cents, at 12 to 35 cents per pound. S3 ° Pounds of butter fat. ^ CI 2 -a- 1,000 900 800 700 600 500 400 300 200 100 $ $ $ % $ « $ $ f $ 12 120.00 108.00 96.00 84.00 72.00 60.00 48.00 36 . 00 24.00 12.001 12 12J 122.50 110.25 98.00 85.75 73.50 61 . 25 49.00 36 . 75 24.50 12.25 Vi\ 12J 125.00 112.50 100.00 87.50 75.00 62.50 50.00 37 . 50 25.00 12.60 m 12? 127.50 114.76 102.00 89.25 76.50 63.75 51.00 38.25 25.50 12.75 12? 13 130.00 117.00 104.00 91.00 78.00 65 . 00 52 . 00 39.00 26 . 00 13.00 13 13:1 132.50 119.25 106.00 92.75 79.50 66.25 53.00 39.75 26 . 50 13.25 131- Vil 135.00 121.50 108.00 94.50 81.00 67 . 50 54.00 40 . 50 27.00 13.50 13i 131 137.50 123.75 110.00 96.25 82.50 68.75 55.00 41 . 25 27 . 50 13.75 13? 14 140.00 120.00 112.00 98.00 84.00 70.00 56.00 42.00 28.00 14.00 14 U\ 142.50 128.25 114.00 99.75 85.50 71.25 57.00 42.75 28.50 14.25~ 14J- 14J 145.00 130.50 116.00 101.50 87. (JO 72.50 58.00 43.50 29.00 14 50 14.1 14| 147.50 132.75 118.00 103.25 88 . 50 73.75 59.00 44 . 25 29 . 50 14.75 14? 15 150.00 135.00 120.00 105.00 90 . 00 75.00 60.00 45.00 30.00 15.00 15 15-4 152.50 137.25 122.00 106.75 91.50 76 . 25 61.00 45.76 30.50 15.25 15J- \^ 155.00 139.50 124.00 108.50 93.00 77 . 50 62.00 46 . 50 31.00 15.50 15i 15?- 157. 5C 141.75 126.00 110.25 94.50 78.75 63 . 00 47 . 25 31.50 15 75 15? 16 160.00 144.00 128.00 112.00 96.00 80.00 64 . 00 48.00 32.00 16.00 16 161 1 62 . 50 146.25 1 30 . 00 113.75 97.50 81.25 05 . 00 48.75 32.50 16.25 16i 16J 165.00 148.5C 132.00 115.50 99.00 82.50 66.00 49.50 33.00 16.50 16i 16? 167.50 150.75 134.00 117.25 100.50 83.75 67.00 50.25 33.50 16.75 16? 17 170.00 153.00 13G.0C 119.00 102.00 85.00 68 . 00 51 . 00 : ;4 . 00 17.00 17 171 172.50 155.25 138.00 120.75 103.50 86 . 25 (59 . 00 51.75 34 . 50 17.25 17i 17i 175.00 157.50 140.00 122.50 105.00 87.50 70.00 52.50 35.00 17.50 17 {■ 17? 177.5(i 159.75 142.00 1 24 . 25 106.5f 87.75 71.00 53.26 35.50 17.75 17? 18 ISO.Of 162.0( 144. Of 1 26 . 00 108 00 90.00 72.00 54. Of 36 . Of 18.00 18 181 182.50 l(il.25 1 46 . OC 127.7r 109.50 91.25 73. Of 54.75 36 . 50 18.25 183- m 185.00 166.5C 148.00 129. 5f 111. Of 92 . 50 74.00 55. 5( 37.00 18.50 18.i IS? 1 87 . 5( 168.75 150. Of 131.25 112. 5f 93.75 75.00 56.25 37 . 50 18.75 18? 1,000 900 800 700 600 500 400 300 200 100 Apfciidix. 217 Table VII. Anuniiif diic for Imttor fal 1 Continued. t.1 Toiiuds of butter fat. tg aS O.S Z-6 3-i ^9 1, 000 900 800 700 600 500 400 300 200 100 & $ ij! $ ^ $ s $ $ $ $ 19 190.00 171.00 152.00 133.00 114.00 95 . 00 76.00 57 . 00 38.00 19.00 19 19} 192.50 173.25 154.00 134.75 115.50 96 . 25 77.00 57 , 75 38 . 50 19.25 19] 19} 195.00 175.50 150.00 136.50 117.00 97 . 50 78 . 00 5S.50 39.00 19.50 19} 195 197.50 177.75 158.00 1 38 . 25 118.50 98.75 79.00 59 . 25 39.50 19.75 195 20 200.00 180.00 160.00 140.00 120.00 100.00 80 . 00 60 . 00 40.00 20 . 00 20 20] 202 . 50 182.25 162.00 141.75 121.50 101.25 81.00 60 . 75 40.50 20.25 20] 20J 205.00 184.50 164.00 143.50 123.00 102,50 82.00 61.50 41.00 20 . 50 20.1, 20| 207 . 50 180.75 166.00 1 45 . 25 124.50 103.75 83.00 62.25 41.50 20.75 20] 21 210.00 1S9.00 168.00 147.00 1 26 . 00 105.00 84.00 63.00 42.00 21.00 21 21-1 212.50 191.25 170.00 148.75 127.50 106.25 85.00 63.75 42.50 21 . 25 2U 21} 215.00 193.50 172.00 150.50 129.00 107.50 86.00 64.50 43.00 21.50 2H 21| 217.50 195.75 174.00 152.25 130.50 108.75 87.00 65.25 43.50 21.75 2Vl OQ 220 . 00 198.00 176.00 154.00 1 32 . 00 110.00 88 . 00 66 . 00 44.00 22 00 22 22] 222.50 200.25 178.00 155.75 133.50 111.25 89.00 66.75 44.50 22.25 22] 22o 225 . 00 202.50 180.00 157.50 135.00 112. 50 90.00 67 . 50 45 . 00 22 . 50 22} 22-"^ 227.50 204.75 182.00 159.25 136.50 113.75 9 1 . 00 68 . 25 45 . 50 22.75 22! 23 230 . 00 207.00 184.00 161.00 138.00 115.00 92.00 69.00 40.00 23.00 23 23] 232 . 50 209 . 25 180.00 162.75 139.50 116.25 93.00 69 . 76 46.50 23 . 25 23] 23i 235.00 211.50 188.00 164.50 141.00 117.50 94.00 70.50 47 . 00 23.50i 23} 23i| 237.50 213.75 190.00 166.25 142.50 118.75 95.00 71.25 47 . 50 23 . 75 237 24 240.00 216.00 192.00 168.00 144.00 120.00 96.00 72.00 48 . 00 24.00 21 243 242 . 50 218.25 194.00 169.75 145.50 121.25 97 . 00 72.75 48.50 24.50 24] 24 J 245.00 220.50 196.00 171 .50 147.00 122.50 98 00 '-.', . 50 49.00 24. 6C 24.', 24^ 247.50 222.75 198.00 173.25 148.50 123.75 99 . 00 74.25 49 . 50 24.75 24]' 25 250.00 225 , 00 200 . 00 175.00 150. oil 125.00 100.00 75.01 50 . 00 25. 0( 25 1,000 900 800 700 lioO 500 400 30O 200 100 (For dirertiuus fur uye, see ]"iagf 'Jlli. 2l8 Tcs/iiii/- Milk (ind /Is Products. Table VIII. Relative value tables. (Fur directions fur irf;, s.:': i;p. ]8('-8]). □ , 0.1 — Pkich IIP ^ n.K I'E i 100 1- JlfNDS, IN DOI, LARS AND <_fiNTS, 3.0 .30 .31 .33 .3 + .30 .37 39 .40 .42 .43 .45 3.1 .31 .33 .34 .36 .37 . 39 ^40 .42 .43 .45 .40 3.2 32 .34 .35 .37 .38 .40 .42 .43 .45 .46 .48 3.3 '.ZV, .35 .30 .38 .40 .41 .43 .45 .46 .48 .49 3 . 1 .34 .30 .37 .39 .41 .42 .44 .40 .48 .49 .51 3.5 .35 .37 . 38 .40 .42 .44 .45 .47 .49 .51 .52 3 . (i .30 .38 .40 .41 .43 .45 .47 .49 .50 .62 .54 3.7 .37 . 30 .41 .43 .44 .40 .48 .50 .52 .54 .55 3.8 .38 .40 .42 .44 .40 .47 .49 .51 . 53 .65 . 0/ 3.!) .3!) .41 .43 .45 .47 .49 .51 .53 .f5 .57 . 58 4.0 .40 .42 .44 .40 .48 .50 .52 .54 .50 .58 . 00 4.1 .41 .43 .45 .47 .49 .51 . 53 .65 .57 .59 .01 4.2 .42 .44 .40 .48 .50 .52 . 55 .57 . 69 .(;i .03 4.3 .43 .45 .47 .49 .52 .54 . 50 . 58 .r,o .62 .04 4.4 .44 .40 .48 .51 . 53 . 55 . '>/ . 59 .02 .64 .00 4.r, .45 .47 .49 .52 .54 . 50 .68 .01 .03 . 6 ) .07 4.0 .40 .48 .51 .53 . 55 .57 .60 ,62 .64 .67 .09 4.7 .47 .09 .52 .54 .60 . 59 .01 .63 .66 .68 .70 4..S .48 .50 .53 .55 . 58 . 00 .02 .65 .67 .70 .72 4.9 .4!) .51 .54 .50 . 59 .Bl .04 .i;o .69 .71 .73 5.0 .50 .52 .55 .57 .00 .02 .05 .07 .70 .72 . 75 5.1 .51 .54 .50 .59 .01 .04 .00 .09 .71 .74 .70 5 . 2 .52 .55 .57 .00 . 02 . 05 .OS .70 .73 .75 .78 5.3 . 53 .50 .58 .01 .04 .60) .09 .72 .74 . / 7 .79 5.4 .54 .57 .59 .02 .65 .07 ,70 .73 .76 .78 .81 5 . 5 . 55 .58 . 00 .03 .00 .09 .71 .71 . 1 / .SO 82 5.0 . 50 .59 .02 .04 .07 .70 . iW .70 .78 .81 .84 5.7 .67 .00 .0.3 . 00 OS .71 .74 .77 .80 .83 .85 5 . s .5.S .01 .01 .07 .70 .72 . / '' .7S .81 .S4 .87 5.0 .50 .O'J .05 .08 .71 .74 .77 .so .83 . S(i .S8 0.0 .00 .(■,;; .00 .(iO 7') . ; •' ) .7s .SI .84 .87 .90 Apfcudi.x. 219 Tiilde VIII. Relative valiio tallies {Continued). 3.0 3.1 •i.-l 3.3 3.4 3.5 3.S 3.9 4.0 4.1 4.2 4.3 4.4 4.5 4.6 4.7 4.8 4.9 5 5.1 5.2 5.3 5.4 5.5 5.6 5.7 5.8 5.9 6.0 Price of milk i'kk IGO polwi^s, in i>oli.ars and tents .46 .48 .49 .51 .52 .54 .55 .57 .58 .48 .511 .51 . o3 .54 .56 . 57 .59 ' .60 1 .50 .51 .53 .54 .56 .58 , 59 .61 . 62 .51 . 'j'^ .54 . 56 .58 . 59 . 6 ; . 63 .64 . 53 .54 .56 .58 .59 .61 .63 .65 .66 .54 .56 .58 . 59 .61 .63 .65 .66 .68 .56 . 58 .59 .61 . 63 . 65 .67 .68 .70 .57 . 59 .61 .63 . 65 .67 . 68 .70 .72 .59 .61 .63 . 65 .66 .68 .70 .72 .74 .60 .62 .64 .66 .68 .70 -0 .74 .76 .62 .64 .66 . 68 .70 .72 .74 .76 .78 .64 . M") .68 .70 .72 .74 .76 .78 .80 . 65 . 67 . 69 .71 . * 3 .76 .78 .80 82 .67 .69 .71 .73 .75 .77 .80 .82 .84 . 68 .70 .73 .75 . 1 1 .79 .81 .84 .86 .70 -.> .74 .76 .79 .81 .83 .85 .88 .71 :74 .,6 .78 .SO .83 .85 .87 . 90 .73 .75 .78 .80 .82 .85 .87 .8'.! .92 .74 77 .79 .82 .84 .86 .89 .91 .94 .76 .78 .81 . 83 .86, .88 .91 .93 .96 . / i .80 82 .85 .87 .90 .92 .95 .97 .79 .82 .84 .87 . 89 . 92 ,94 .97 .99 .81 .83 .86 .88 .91 . 94 . 96 . 99 i .01 .83 .85 .87 .90 .93 . 95 .98 1.01 1.03 .84 .86 .89 .92 .91 .97 1.00 1.03 1.05 .85 .88 .91 . 93 .96 99 1.02 1.04 1.07 .87 . 90 .92 .95 . 1'8 1.01 1.04 1 . 06 1 . 09 .88 .91 .94 .97 i!oo 1.03 1.05 1.08 1.11 .90 .93 .96 .99 1.01 1.04 1.07 1.10 1.13 .91 .94 .97 1.00 1.03 1.06 1.09 1.12 1.15 .93 .96. .99 1 . 02 1.05 1.08 1.11 1.14 1.17 . 60 .62 .H4 .66 .68 .70 72 ^74 .76 .78 .82 .84 ..S6 .88 .90 .92 .94 .96 .98 1.00 1.02 .04 1.06 1.08 1.10 1.12 1.14 1.16 1.18 1.20 2 20 Tcsiino- Milk and lis Products. Table Vlir. Reliitive value tables [Continued). Feice OF SULK I'ICR 100 I'0UND3. IN DOLLARS AND CENTS. 3.0 .61 .63 .64 .66 .67 .69 .70 .72 . / o .75 3.1 .64 .65 .67 .68 .70 .71 73 .74 .76 .78 3.2 .66 .67 .69 .70 .72 .74 ^75 . / / .78 .80 3.3 .68 .69 .71 . 73 .74 .76 .78 .79 .81 .83 3.4 .70 .71 .73 .75 .76 .78 .80 .82 .83 .85 3.5 . * li .73 .75 .77 .79 .80 .82 .84 .86 .88 3.6 .74 .76 . / / .79 .81 .83 85 .86 .88 .90 M . 7 .76 78 .80 .81 .83 .85 .87 .89 .91 .93 .3 . 8 .78 .80 .82 .S4 .85 .87 .89 .91 . 93 .95 3.9 .80 .82 .84 .86 .88 .90 .92 .94 .96 .98 4.0 .82 .84 .86 .88 .90 .92 .94 .96 .98 1.00 4.1 .84 .86 .88 .90 .92 .94 .96 .98 1.00 1.03 4.2 .86 .88 .90 .92 .94 .97 99 1.01 1.03 1.05 4.3 .88 .90 .92 . 95 .97 .99 1.01 1.03 1.05 1.08 4.4 .90 .92 .95 .97 .99 1.01 1.03 1.06 1.08 1.10 4.5 92 .94 .97 .99 1.01 1.03 1.06 1.08 1.10 1.13 4.G .94 .97 .99 1.01 1.03 1.06 1.08 1.10 1.13 1.15 4.7 .96 99 1.01 1.03 1 06 1.08 1.10 1.13 1.15 1.18 4.8 .98 1.01 1.03 1.06 1.08 1.10 1.13 1.15 1.18 1.20 4.9 1.00 1.03 1.05 1.08 1.10 1.13 1.15 1.18 1.20 1.23 5.0 1.02 1.05 1 07 1.10 1.12 1.15 1.18 1.20 1 . 23 1.25 5.1 1.05 1.07 1.10 1.12 1.15 1.17 1.20 1 oo 1.25 1.27 5.2 1.07 1.09 1.12 1.14 1.17 1.20 1.22 l'25 1.27 1.30 5.3 1.09 l.U 1.14 1.17 1.19 1.22 1 . 25 1.27 1.30 1.32 5.4 1.11 1.13 1.16 1.19 1.21 1.24 1.27 1.30 1.32 1.35 5.5 1.13 1.15 1.18 1.21 1.24 1.26 1.29 1.32 1,35 1.38 5.6 1.15 1.18 1.20 1.23 1.26 1.29 1.32 1.34 1.37 1.40 5.7 1.17 1.20 1.23 1.25 1.28 1.31 1.34 1 . 37 1.39 1.43 5.8 1.19 1.22 1.25 1.28 1.30 1.33 1 . 36 1 . 39 1.42 1 . 45 5.9 1.21 1.24 1.27 1 . 30 1.33 1.36 1 . 39 1.42 1.45 1.48 C.O 1 . 23 1.26 1 .29 1.32 1.35 1.38 1.41 1.44 1.47 1.50 Ap_pemb'x. 221 'aide VIII. Relative value tallies [Continued] - Price MF MILK PEK ]Oil pr.tL'NDS, IN LiOr.LA ;.< .\NI, ■ENT = , 3.0 .7ti .78 .79 .81 .82 .84 .85 .87 ,88 .90 :;.! .79 .81 .82 S4 . 8.'i .87 .88 . 90 ,01 . '. 1 ■ '■ 3.ii .82 .83 .85 .80, .88 .90 .91 ,93 .94 . ,10, 3 . 3 .84 . SO .87 . 8; 1 .91 .92 .94 , 90 ,97 99 3.4 .87 .88 . 90 '.]'! . 93 .95 ,97 .99 1 , 00 1 , 02 3.5 . 89 .91 . 93 .94 . 90 .98 1,00 1.01 1 , 03 1.05 3.0 .92 .94 . 95 .97 .99 1 , 00 1,0:'. 1.04 1 . 00 1.08 3 . i .94 . 90 . 98 1.00 1.02 1,03 1 . 05 1.07 1 . 09 1.11 3.8 .97 . 99 1.01 1.0:; 1.04 1.00 1 . 08 1 . 10 1.12 1 .14 3.9 .99 1.01 1 . 03 1 . 05 1.07 1 . 09 1.11 1.13 1.15 1.17 4.0 1.02 1.04 1.06 1.08 1.10 1,12 1.14 1.16 1.18 1.20 4.1 1.05 1.07 1.09 1.11 1.13 1.15 1.17 1.19 1.21 1.23 4.2 1.07 1.09 1.11 1.13 1.15 1 .18 1 . 20 1.22 1 .24 1.20 4.3 1.10 1.12 1.14 1.10 1.18 1,20 1.2.3 1 . 25 1.27 1.29 4.4 1.12 1.14 1.17 1.19 1.21 1 . 23 1 . 25 1.28 1.30 1 . 32 4.5 1.15 1.17 1 .19 1.21 1.24 1.20 1.28 1.30 1.33 1.35 4.0 1.17 1.20 1 . 22 1 . 24 1 . 20 1 . 29 1.31 1.33 1 . 36 1 . 38 4.7 1.20 1 22 1.25 1.27 1.29 1.32 1 . 34 1.36 1..39 1.41 4.S 1.22 L25 1.27 1..30 1.32 1.34 1 . 37 1,39 1.42 1.44 4.9 1 . 25 1.27 1 . 30 1 . 32 1 . 35 1.37 1.40 1.42 1.45 1.47 5.0 1.27 1 . 30 1.32 1 . 35 1.37 1.40 1,42 1.45 1.47 1 . 50 5.1 1.30 1.33 1.35 1.38 1.40 1.43 1.45 1.48 1.50 1 . 5:'. 5.2 1 . 33 1.35 1.37 1.40 1.43 1 . 46 1.48 1.51 1 . 53 1 . 50 5.3 1.35 1.38 1 . 40 1 . 43 1 . 40 1.48 1.51 1,54 1.56 1.59 5.4 1.38 1.40 1.43 1 . 40 1.48 1.51 1.54 1.57 1.59 1.62 5.5 1.40 1.43 1 . 40 1.48 1.51 1.54 1.57 1 . (lO 1 . 62 1 . 65 5.(i 1.43 1.46 1.48 1.51 1.54 1.57 1 . 0,0 1 . 62 1 . 05 1 . 68 5.7 1.45 1.48 1.51 1.54 1.57 1 . 00 1 . 02 1.65 1 . t:,^ 1.71 5.8 1.48 1.51 1.54 1.57 1.59 1.62 1 . 05 1 . 68 1.71 1.74 5.9 1.50 1.53 1.50 1.59 1.62 1.65 1.68 1.71 1.74 1,77 6.0 1.53 1.50 1.59 1.02 1.65 1.0,8 1.71 1,74 1.77 1 . SO Tcs/iii!'- Milk and Its Prodiirls. Table IX. Butter chart, showing calculated yield of but= ter (in lbs.) from i to 10,000 lbs. of milk, testing 3.0 to 5.3 per cent. (Se(! directions for use, p. 211). H :; . 00 :;.io :!.20 3 . 30 3 . 40 :i.50 3 . 60 3,7( 3 . 8f 3.90 4.00 4.1( H Milk i Mlik lb3. lbs 10, Ono .32.5! .336 318 360 371 383 394 406 418 429 441 452 10, 000 9, 000 293 302' 3131 324^ 334' 345 355 1365 376 386 397 407 9, 000 8, 000 260 269 278 288 297 306 31.V 325 334 :;43 3-53 362 8,000 7, 000 228; 235; 244 252 260' 268 276 284 293 300 .309 316 7, 000 6,000 196 202 209 216 22:-! ; 230 236 244 251 2.57 265 271 6, 000 .5, 000 163' 168 174 180 186; 192 197 203 209 216 221 226 5, 000 4, 000 130 134 139 144' 148' 1.53 158 102 167 172 176 181 4, 000 Z, OCO '.17.5 101 104; 108 111 115 118: 122 125 129 132 1.36 3, 000 2,000 65.0 67.2 69.0 72.0 74.2 76.6 78.8 81.2 83.6 85.8 88.2 90.4 2, 000 1,000 32.5 33.6.34.8 :i6. 0.37. 1,38. 3 39.4I4O.6 41.8 43,9 44.1 45.2, 1,000 900 29.3.30.2 31.3 .32.4.33.4.34.5 .35.5!:!6.5 37.6 :3S.6 .39.7 40.7' 900 800 26.026.927.828.829.7.30.6 31.5.32.5 33 . 4 34 . 3 35 . 3 36.2 800 700 22.823.524.4 25.2 26.0|26.8 27.6 28.4 29.3 .30.0 .30.9 Jl.C 700 600 19.520.220.9 21.6 22.3|23.0 23.6 24.4 25.1 25.7 26.5 27.1 600 500 10.316.817.4 18.0'l8.619.2 19.7:20.3 20.9 21.5 22.1 22.6' 500 400 13.0 13.4 1.3.9 14.4 14.8 1.5.3 15.8 16.2 10.7 17.2 17.6 18.1 400 .300 9.7 10.1 10.410.811.1 11.5 11.812.2 12.6 12.9 13.2 13.6 300 200 6.5; 6.7i 6.9 7.2, 7.4 7.6 7.9, 8.1 8.3 8.6 8.8 9.0 200 100 3.2! 3.4 1 3.5 3.6: 3.71 3.8 3.9 4.1 4.2 4.3 4.4 4.5, 100 90 2 9 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 3 9 4.1 90 80 2.6 2.7J 2.8 2.9 3.0 3.1 3.2 3.3 3.4 3.4 3.5 3.6 80 70 2.3: 2.3 2.4 2.5 2.6 2.7 2.8 2.8 2.9 3.0 3.1 3.2 70 60 1.9 2.0 2.1 2.2 2.2 2.3 2.4 2.4 2.5 2 . 6 2.7 2.7 60 50 1.6' 1.7 ].7i 1.8' 1.9' 1.9 2.0 2.0 2.1 2.2 2 2 2 . 3 50 40 1.3 1.3 1.4 1.4 1.5 1.5 1.6 1.6 1.7 1.7 \'^i 1.8 40 30 1.0 1.0 1.0 1.1 1.1 1.2 1.2 1.2 1.3 ].:: 1.3 1.4 30 20 .6 .7 .7 .7 .7 .8 8 .8 .8 ,9 .'.1 .1.1 20 10 .3 .3j .4 .4 .4 .4 .4, ,4 4 .4 .4 , .5 10 9 3 .3 .3 .3 .3 .3 .4' .4 .4 . 1 .4 .4 9 X '3 :.: ':■', ^3 .:-; .3 . 3 . 3 .;:; _ :; A .4 8 7 .2 .2 .2 .3 ..3 .;'. . :; . '■'' .:; . .3 . !■> 7 6 2 2' 2 2. 2 2 . 2 . 2 , :; .:; . ;> ..'1 6 .5 1 . ] .1 1 .2 .2 .2 M ''i •_^ ;: 2 2 5 4 ] .1 -1 ,1 .1 .1 .1 .1 .1 .1 .] .1 .1 1 .1 .1 .1 .1 .1 .1 .1 .1 .1 .1 .1 .1 1 H .■',.00 3.10 3.20 3.30::. 10.3.. ^,0 3.60 3.70 !.80 3 . 90 4.00 4.10 H Appendix. Tablo rX. BiiUer fliarl i Continued] "4 . 20'4 . 30 4 . 404 . 50 4 . CO 4.7(1 4 . SO 4 . 00 5 . 00 o .10 5 . 'Jn 5 . 30 Milk ll's. 10,000 4G4 470 4S7 4011 r,10 9,0001 41S 4'J,^ 4:W 440 4ri9 47( 8,000 371 3S1 300 3011 40.o 3 . G .3.8 3.9 4.0 4.1 4.2 4 . 3 4.4 4.. 5 4.6 4.7 4.S 4.9 '>.\ 5.2 5.3 5.4 5.5 5.fi 5.7 5.S 5.9 CO .10 3.30 3.41 3.52 3.63 3.74 3.S5 3 . 96 4.07 4.18 4.29 4.40 4.51 4.62 4.73 4 . Nl 4 . 95 5 . 06 5.17 5.28 5.. 39 5 . 50 5.61 5.72 5.83 5.94 6.05 0.16 6.27 6.38 6.49 60 1.11 3.33 3.44 3.55 3.66 3.77 .3.89 4.00 1 .11 4.22 4.33 .12 3.. 36 3.47 3.-58 3.70 3.81 3.92 4 . 03 4.14 4.20 4.. 37 4.444.48 4 . 55 4 . 59 4.66 4.77 4.70 4.82 4.884.9: I .13 1.11 3.. 39 3.42 3.50 3.53 3.62 3.65 3.733.76 3.84 3.88 5.00 5.11 5.22 5. 5.44 5 . 55 5.66 5.77 5.88 5 . 99 6.11 (i.22 6 . 33 6.44 6.55 6 6i; .04 .16 .26 5.38 5.49 5 . 60 5.71 5.82 5 . 94 (i.O. i;.]6 6. 6 . 38 6 . 50 6.61 3.96 4.07 4.18 4.29 4.41 4.52 4 . 63 4.75 4.86 4.97 5 . 09 5 . 20 5.31 5.42 5 . 54 5.65 5.70 5 . 88 '5 . 99 0,10 6.22 6.3.3 6 . 44 6 . 55 6.67 6.7S 3.99 4.10 4 . 22 4.33 4.45 4.56 4.67 4.79 4.90 5 . 02 5.13 5.24 5.. 36 5.47 5 . 59 1 . 1 5 1 . 1 ( 3.45 3.48 3 . 57 3 . 60 68 3.71 80 3.83 3.913.94 4.03 4.00 4.14 4 . 26 4 . 37 4.49 4 . 60 4.72 4.83 4.95 5 . 06 5.18 5 . 29 5.4 5.52 5.64 5.705.75 5.81 5.8' 5.93 5.98 6.04 6.10 6.166.21 6. 27 '6.33 6.3s'6.44 6.50'6.56 6.61 6.67 6. 731;. 79 6, ,s I 6. '.10 18 29 4.41 4 . 52 64 76 4.87 4.99 5.10 5 . 22 5.. 34 5.45 'i . 57 5 . 68 5 . 80 5.92 6.03 6.15 6.2( 6 . 38 6 . 50 r,.61 6.7.3 6., SI i.r 51 3.63 3.74 3.86 3 . 98 4.10 4.21 4.33 4.45 4.56 3.65 4.80 4.91 5 , 03 15 5.38 5 . 49 5.62 5.73 5 . .s5 5.97 6 . 08 6 . 20 6. 1.18 54 3.66 78 89 4.01 4.13 4.25 4.. 37 4.48 4 . 60 4.72 4.84 4 . 96 07 19 5 . 43 5 . 55 5 . 66 5.78 5.90 6,. 02 6.14 6.25 6.37 6.49 6.61 1 19 57 -J 68 V, 81 ?, 93 4 05 4 17 4 28 4 40 4 52 4 64 4 76 4 88 '0 00 5 12 •J 24 5 36 1.20' .79 6.84 .90 6. '.16 .02 7.(l.s 60 3.72 84 96 4.08 4.26i 4.32, 4.44 4.56' 4.68! 4.S0| 4.92' 5.04 5.161 5. 28 1 5.4715.52 5.59|5.64 5.71 5.76 5.83 5.88 5 . 95 6.07 6.19 6.. 31 6, . 43 6 . 55 6 . 66 6.7S 6 . 90 7.02 7.14 3.0 3.1 3.4 3.6 3 . 7 3.8 3.9 4.0 4.1 4.2 4.3 4.4 4.5 4.6 4.7 4.8 4.9 6.001 5.0 6.12n 5.1 6.24| 5.2 6 . 36' 6.48' 5.4 6.60 6. 6.84 . 96 7 . OS 7.20 - '/'/''" '"''''-^ ^25 Table XI. Yield of cheese, corresponding to 2.5 to 6 percent of fat with lactometer readings 26 to 36. (See p IT-I). L.^i'ToMETRi: DEGRIiES. -' "5 26 27 28 29 30 3 1 32 33 34 35 :iG •2.0 7 . 2> 7.41 7.51 7.67 7.81 7. '.11, S.07 S.20 s. ;-;:;; s.47 s.oo 2 . r> 7.44 7 . 57 7.70 7. S3 7 91; S.O'.I s 22 8 ■ ) - 1 1 S 40. S 02, S.76, '2 7 7.5;) 7 72 7 .So 7 .'.I'.i S 12 S.l!.) S ■ Is s 51 ' s 64 S 77 S.'.U •2 . S 7.74 7 .S7 s.oo! S. 14 S ■_>; s.4{il s .-J 8 67' S SO' s 9) 0.07" 2.9 7.!ii/ s.(i>; s. I6i s. :;o 8 31 S.56i S 69 s S2l 8 05' 00 '..' . 22 3.0 8.0.-) s . 1 s s.:;i| s. 15 S 5S S.71 s SI 8 071 ',1 11| 24 0.37 3.1 S.21 s.:;4 8. 17 8.60 s 74 s S7 11 (.10 13; 26 :.;',! 9.5:;|| '■> '^ s hi; S.49 s . 62 8.75 S sw 0.02; 9 15 li^ 'J !■■ 9 0.0S| 3^3 8.52 s.i;.-, S.7sl S.'.il' ',.1 0"i o.is' :;i 44' '.1 57' '.1 70 0.S4 3.4 8.67 '< 0.4SI 9 62 751 SS 10 01 10.15!! 3.6 8 9S 9.11 6.-J4| ll.:;7| 11 50 '.i.6:;| 9 ',IO|10 0:; 10 17 10.30I 3.7 9,i:'. 9.26 !l 65 ',i,7S 9 !I2 10 05 10 ]'.l 10 ' '. ■ ' 10.46i| 3 . S 9 . 29 '.1.42 9.55 IM'iS 9 sl 0.9440 OS 10 21'! 34 10 48 10.61, 3.9 9.44 il.57 '.1.70 8 . 84 9 '.'7 Ml. 10 10 2" 10 :;o 10 ..0 10 64 10.77' 4.0 9.60 9.73 '.).86 10.00 10 13 10.26 10 :'.9 10 5:! 1 i;6 10 79 10.9::'l 4.1 9.75 9.8S 10.02 10.15 10 2S 10.30,10 54 10 r.s 10 Sl 10 94 1 1 osl 4.2 9 90 10.03 10.17 10.30 10 43 10.57 10 70 10 84|10 07 11 10 11.24(1 4.3 10.06 10.19 10.32 10.45 10 5S 10.7210 85 10 994 1 12 11 25 11 .39, 4.4 10.21 10.34 10.48 10.61 10 74 IO.S7I1I 00 n 14 11 27 11 41 11.55 4.5 10.36 10.49 10.63 10.76 10 79 11.03|ll 16 11 20' 11 42 11 56 11.70 4.6 10.52 10.65 10.78 10.92 11 05 11.18|11 31 11 4541 58 11 71 11.85l| 4.7 10.67 10.81 1 . 94 11.07 11 20 U 34|11 47 11 6011 73,11 87 12.01i| 4.8 10.83 10.96 11.09 11.22 11 36 11.4911 62 11 76,11 SO 12 02 12.16 4.9 10. 9~ 11.11 11.25 11.38 11 51 11.65ln 7s 11 91 12 04 12 18 12.32 5.0 11.14 11.27 11.40 11.54 11 67 n.80lll 93 12 07 12 20 12 34 12.48 5.1 n.29 11.42 11.55 11.69 11 82 Il.!l6il2 09 12 23 1 '^ 36 12 49 12.63 5.2 11.45 II.5.S 11,71 1 1 . 85 11 98 12.1M2 24 12 .■■;s 1 '^ 52 12 66 12.80 5.3 11.60 11.73 11.86. 1 1 . 99 12 13 12.2712 40 12 53 12 67 12 71 12.85 5.4 11.76 11.89 12.02 12.16 12 29 I2.42'r2 55 12 69 TO 83 12 97 13.01 5.5 11.91 12.04 12.17 12.31 12 44 12.5sll2 71 12 85 ]0 99 13 12 13.25 5.6 12.07 12.20 12.33 12.47 12 60 I2.73lr2 87 13 00 13 14 13 28 13.41 5.7 12.22 12.35 12.48 12.52 12 75 12.S9il3 02 13 16 30 13 44 13.57 5.8 12.38 12.51 12.64 12.77 12 91 13.05|13 is 13 ."1 13 15, ly 59 13.72 5.9 12.53 12.66 ll.79'l2.93 13 06 13.19,13 33 13 17 6.0 13 74 13.87 6.0 ' 12.69 12.82 12.95 1 2 . 09 1 3 -)0 13. 35' 13 4'l 13 62 13 75 13 89 14.02 2 26 Tcsli Milk and Its Producls. Table XII. Comparisons of Farenheit and centigrade (Celsius) thermometer scales. Fiihren- Centi- Fahren- (Jenti- Faliren- Centi- lieit. graile. lieit. graile. licit. grade. + 212 + 100 + 170 + 80 + 140 + 60 211 99.44 175 79.44 1.39 •59.44 210 98.89 174 78.89 138 58 . 89 209 98 . 33 173 78.33 1.37 .58.33 208 97.78 172 77.78 1 30 .57 . 78 207 97.22 171 77.22 135 .57.22 200 96 . 07 170 70.07 1.54 60 . 67 20.-. 90 . 1 1 169 70.11 153 .56.11 204 95.55 108 75.55 1.52 .55.55 203 95 167 75 131 55 202 94.44 166 74.44 130 .54.44 201 93 . 89 105 73.89 1 29 .53.89 200 93.. 33 164 72.33 128 .53.33 ]~> culj. meter. 352,j hectoliter. 20 57 c. c. 9404 liter. o -J 7854 liters. 04.8 milHgrams. 2,8 35 grams. 4536 kilogram. [r-lrir wci^ljt'i arjil m.jaMirc-s. 1 meter 1 meter 1 kilometer 1 S(|. centimeter. 1 ?i|uare meter.. 1 square meter., 1 hectare 1 c. c 1 cub. flecimeter, 1 cul). meter 1 hectoliter 1 c. c 1 liter 1 decaliter 1 gram 1 gram 1 kilogram I -.toiiiary .37 inclics. ()!):iO vards. 021 f mile. 1 55 sq. incli. 70 t sq feet. ] '.10 sq. 3'ards. 471 acres. Ot>l cul)ic inch. 02.3 culjic inches. 314 cub. feet. 8377 bushels. 0338 fluid ounce. .flv5G7 Cjuarts. 0417 quarts. .43. grains. 0.3.5274 oLuice. 2046 pounds ( av. 1228 1 Afpcndix. 229 SUGGESTIONS regarding the organization of co= operative creameries and cheese factories. When the farmers of a neighborhood are considering the estab- hshment of a creamery or cheese factory, they should first of all make an accurate canvasof the locality to ascertain the number of cows that can be depended on to supply the factory with milk. The area which ma^^ be drawn from, will vary according to the kind of factor}' which it is desired to operate. A successful sepa- rator creamer^' will need at least -tOO cows within a radius of four to five miles from the proposed factor}'.* Snaall cheese fac- tories can be operated with less milk, and gathered-cream and butter factories, generally cover a much larger territory than that inentioned. In all cases, however, the question of the number of cows con- tributing to the enterprise must be fully settled before further steps are taken, since this is the vital point, and one upon which success will largely depend. Methods of organization. The farmers should form their own organization, and not accept articles of agreement proposed by traveling agents. An agreement to supply milk from a stated number of cows should be signed bj' all who e.xpect to join the association. When a sufficient number of cows has been pledged to insure the successful operation of a factory, the larmers agree- ing to supply milk should meet and form an organization. This may be done according to either of the following plans which have been known to give good satisfaction. Raising money for building and equipment. First. — Each member will sign an agreement to pay on or be- fore a given date for a certain number of shares or stock in the company at dollars per share ; or. Second.— .\n elected board of directors may be authorized to borrow a sum of monev not exceedieg thousand dollars on their individual responsibility, and the sum of cents, (usualh' five cents I per hundred pounds of milk received at the factory shall be reserved for the payment of this borrowed money. ' Bull, ofj, Wisconsin experiment station. 230 Tcslino- Milk and lis Products. Coiisl it iitioii and liy-lii«s of co-o]jcrativc associations are flr.'iwii 11]) and sif^ned Ijy tlie ]jrospcctive members of the associa- tion as soon as possil)le after it lias fieeii determinefl to form such cin association. iVs it is imiiossiblc to include in an illustrtition all the articles and rules th<-i.t may fie found useful in each partic- ular instance, the following suggestions in regard to some of the points to be included in the doeumeots are given as a guide only. It may be found advisable to modify them in -i-arious ways to meet the needs of the organization to be formed. After the constitution and b3'-laws have been drawn up and made plain to all the members of the association, they should be jjrinted and copies distributed to all parties interested. CONSTITUTION' OR Articles of Agreement oe the Associatio.n-.' 1. The undersigned, residents within the counties of , state of hereby agree to become members of the co-operative association, which is formed for the jjurjiose of man- ufacturing butter or cheese from whole milk. 2. The regular meetings of the association shall be held annu- ally on the day of the month of Special meetings may be called by the president, or on written ref|uest ol one-third of the members of the association, provided three days' notice of such meeting is sent to all memfiers. Meetings of the board of directors may be called in the same way, either by the president, or by any two members of the board of directors. '.',. The members of the association, or three of the board of directors, shall constitute a r|uorum for the transaction of busi- ness. 4. The officers of the association shall include president, secre- tary, treasurer, one of whom is also elected manager, and these officers together with three other members of the association * The followiuy j>ijl>lic:tt i'lus liave heeti freely ij,beii in prt-pariug this eon.stitii- tioD and by-laws: WoM, Ilaudbook f. Farmers aod Dairyrueu; Minn, exporiment station, bull. No. 3.5; Ontario Agricultural College, sjiecial bulletin, May 1897. Affoidix. 231 shall constitute the board of directors. Each of these six officers shall be elected at the annual meeting and hold office for one 3'ear, or until their successors have been elected and qualified. Any va- cancies in the board of directors ma\' be filled by the directors un- til the next annual meeting of the association. 5. The duties of the president shall be to preside at all meet- ings of the association, and perform the usual duties of such pre- siding officers. He shall sign all drafts and documents of any kind relating to the business of the association, and pay all money which comes into his possession by virtue of his office, to the treasurer, taking his receipt therefor. He shall call special meetings of the association whenever it is deemed necessarj'. In the absence of the president, one of the board of directors shall temporarihr fill the position. 6. The secretary shall attend all business meetings of the asso- ciation and of the board of directors, and shall keep a careful rec- ord of the minutes of the meetings. He shall also give notices of all meetings and all appointments on committees, etc. He shall sign all papers issued, conduct the correspondence and general business of the association, and keep a correct financial account between the association and its members. He shall have charge of all property of the association, not otherwise disposed of, give bonds for the faithful performance of his duties, and receive such compensation for his services as the board of directors maj' deter- mine. 7. The treasurer shall receive and give receipt for all money belonging to the association, and pay out the same upon orders signed by the president and the secretarj-. He shall give such bonds as the board of directors vas^y recjuire. 8. The board of directors shall audit the accounts of the asso- ciation, invests its funds, ajjpoiQt agents, and determine all com- pensations. They shall prescribe and enforce the rules and regula- tions of the factorj'. They shall cause to be kept a record of the weights and tests of the milk or cream recei%'ed from each patron, the products sold, the running expenses, etc., and shall divide among the patrons the money due them each month. They shall 232 Tcsliiii^- Milk and /Is Products. also make some provision for the withdrawal of anymember from the association, and make a report in detail to the association at the annual meeting. Snch report shall include the gross amount of milk handled during the year, the receipts from prrj- ducts sold, anJ all other receii)ts, the amount paid for milk, also for running expenses, and a com[)]ete statement of all other methods pertaining to the business association. 9. Among the rules and regulations to be enforced by the board of directors may be included some or all of the following : a. Patrons shall furnish all the milk from allthecows promised at the organization of the association. b. Onl^' sweet and pure milk will ht accepted at the factor_v, and any tainted or sour milk milk shall be refused. c. The milk of each patron shall be tested at least three times a month. d. ;\n3- patron proved to be guilty of watering, skimming or otherwise adulterating the milk sent to the factor}', or by taking more than SO pounds of skim milk or whey for every 100 pounds of whole milk delivered to the factory, shall be fined as agreed tjy the association. e. A patron's premises may be inspected at any time by the board of directors, or their authorized agent, for the purpose of suggesting improvements in the methods ol caring for the milk or the cows, in drainage and general cleanliness; or to secure sam- ples of the milk of his cows tor examination when it is deemed necessary. 10. Any changes or amendments to the by-laws or constitu- tion of the association must be made in writing by the parties proposing the same, and posted prominoitly in a conspicuous place at the creamery at least two weeks jircvious to their being acted u|)on. Such changes to be in lorce must be adopted by a two-thirds vote of the stockholders. 11. In voting at any annual or special meeting ot the associa- tion, the mi'ml)L-rs shall be entitled to one vote for each cow sup- plying milk to the factory, or for e.ich share of the stock owned by them, as agreed upon. NDEX. The uumljers refer Acid measuri'S, ■J-. Acidity of cream, 104; estimation of, lOi;. Acidity of milk, cause of, 04; determina- tion of. 04, 105: metliods oftesting, 9.',. Adulteratiou of milk, 8S; calculation of, 00. Adulterated butter, 200: cheese, 2u;i. Albumen lo, determiDation of, in milk, 101, 103. Alliaminoids, 13. Albumose, 14. Alkaline tablet test, 09. Alkaline tal)s, 100. Am]iliotcric reaction of milk, 04. Appendix, 20.j. ArtifiL-ial batter, delection of, L'un. Ash, determintitioti of. in buitur, ]'.7; determination of specific gravity, 20n; volatile fatty adds, 2ui; exjiansion co- efficient, 33; priee per pound, 174; table showing amounts due for, at 12 to 25 eents )ier pound, 21i>; test and yield of butter, 160. Buiter milk, Babco^-k test for, 74, 77; chemical analysis of, l'.t7: composition of, 2J0. CaloLilation of adulteralion, 00; of milk solids, S5; of yield of Initter, 160, 167, 169; of cheese, 171; of dividends, at creameries, 174; at eheese factories, 182. f'aliljration of" glassware, 4.''. Carbohydrates, 15, Casein, 13; determination (A, in Initter, 108; in cheese, 203; in milk, 101, 102. Centrifugal machines, 47. Cliamberland fillers, 14. Cheese, 77; calculating yield of, from casein and fat, 17:;; from fat, 171: f/om FOlids not fat and fat, 171, eomposhioD, 205: chemical analysis of, 202: deter- mination of ash, 26^;; casein, 2')3: fat, 202; water, 202: 'iilled", detection of, 203; sampling. 77; yield, calculation of. 160,171; yield of, and -quality of milk, relation between, 171. Cheese factories, calculating dividends at, lS2;co-operative, ls5; proprietary, 184. Chemical analysis of butter, VJ~, 190: butter milk, 107; cheese, 202: milk, 186; skim milk, 197; whey, 197. Cholesterin in milk. 10. Citric acid in milk, 19. Cleaning solutions for test of iiottles, 30. Cleaning test bottles, 30; apparatus for, 38. Cochran's test, 4. Coloring matter, foreign, in milk, de- tection of, 92. Colostrum milk, 10; comjiosition of, 20.".. Composite samples, 134, care of, 143; 234 Tcslintc Milk and lis Products. CUSr for Ik.Mmil', \'u. Tii.'lliu'ls (,f luk- iDK, l-'-l; |.r<>si-i'vativ'_'H lor, 140. Composile satupling, liy use of drip f;;-irii|.le, KSi'.: one-tliinl .sample pipett*.'. i;;s; Sovell HJimplinK tul>e, \'.\^\\ tirj dipper, 134. f 'oinpositiou of butler. 205; liutter luilk, 205; cheese, 205; colostrum milk, 20.^; cream, 20'); milk, 205; skim milk, 205; whey, 205. Condensed milk, composition of, L'li.O; testing of, 79. €onve^.^ion factor for liutter fat, 107. Conversion tallies for thermometer scales, 22<'i; for weitjtils and measures, 22 H. Cow. a, when to lest, 123. Co PCS, nuiniicTof tests required in test- ing, 121. Cows' milk, composition of, 20.^, Cream, acidity of, 104; avoiding errors of Tiieasiiring in testing, 07; i-5ali''ijck test for, I.'pI; hoLtli-s, the Inil h-neckcl, O.S: the Winton , ';'.!; care in samiding, nccfs>ity of. 154; determination of acidity \\\, 100, 108; errors of mi/asur- ing in testing, 05: separation of, in- fluence of temjjerature, 159; Bfjaces, 15(1; specific gravity, 06; testing. 04; testing out fit, 155; testing at cream- eries, 150; use of 5 c. c pipette in, 71; UHC of milk test Vjottles in, (;9: test hi>t W'"^ 07-. weighing in cream testing. 71; weight delivered by a 17.0 r<-. pi- pette. 00. Creameries, calci dating dividends at, 174, 176; co-Ofierative, 175; eream rest- ing at, 150; proi»rietary, 175. Creamery inch, 1 , Curd test, the Wisconsin. 111. Dclyival's bulyromeler. H . Devarda's atidimeter, If'.i. Diameter of testtsr and SjKH'd reijuired . relation bctwiMMi, :m. Dividends. ..■alniiat ing, at elieese factories. X'^'l-, at cr<'ar]u;t i.'s, 171. I 'raining rark for tfst ).oi I Ics, :;!i. J.x,,;., ethcicnt for butter fat, :;rj. i'ailyer and Willard's te.sf, I. !■ arringtoti's alkaline tablet test, '.I'J. i-'at, 12; color of, an iridc.x to slr<;ng(h of acid used, 5S; content, causijs of variation in, 120; determination of, in bulier, 108; in cheH.4e, 202; in milk, 190; gloljules, 12; iufluenee of tem- perature on separation of, 59; measur- ing of, in cream testing. ?■!; in milk testing, 32; pounds in 1-10,000 lbs. of milk, testing 3 to 5.35 jier cent., 212; sjH-cil re*|uired for complete sr>(para- 1 iou of, !>;. Fermentation test, the, 113. I'^illed cheese, detection of, 203. Fjord's centrifugal cream test, 9. Fluorids, detection of, in milk, 190. I'Vjod, influence of, on quality of rjiilk. 131. J'Vjol pipettes, 40. Formaline, detei;tion of, in rjiilk, 197. l-'rozen milk, sani idling of, 24 (iaugcs of crf'aijj. b'lO. Gerber's acid-buty romei er, 7; lerrncnta- tion test, 1 13. 'ilassware used in tlie liabcock test, 34; calibration of, 43. Globulin, 14. Glycerides of fatty adds, 12. Goat cheese. 1 4. Grain leijding, heavy, induence of. on quality of milk, 130. Hand b-stcrs. 52. Ilerni-alliumo^e, 1 1, Herd milk, varial ions in, 12^; ranges in variation, ol I 2,1, Ilypoxanthir], 19. lilt rodueli.jii, ] . Iowa station test, 5. Lactic acid in milk, 16. LactoiTite, 5. Lactose, 15. I.,actochromc, 10. I.,artometer, the, and its application, >^0; degrc-cs, HI; N. V. board of health, ,S3; 'juevcnnc, HO; reading the, 84; time of Liking j-cadings, H5, Index Lecithin in milk, 19. Lefi'manu and Beam test.o, Lesal standards for milk, ^(',i, -i')'^i. Lieltfirmann's metliud, ■",. Manns' tfst, U6. ileasuriij^r fat column in tc.sting Lream, 7;:i; ialestiug milk, 32. MeiCLiry, calibration witli,4li; clt'aniuL;, ■i-1. r^Ietric ami i ustomary sy.stems of weigliLs and measures. coni|iari.son of, 228. Milk, acidity of, 94; adulteration of. S^; amphuteric reaction of, 91; ash, com- position of, IS; chemical analysis of, 186; cholesterin in, 19; citric acid in, 19; colostrum, 19; composition of, 11; table showing composition of, 205; composite sampling of, 134; condensed, 79, 20o; correction tahle for specific gravity of, 208; detection of preserva- tives in, 109; determinationof acidity, 106, 195; of ash, 194; of casein and albumen, 191, 192, 193; of fat, 190; of milk sugar, 193; of specific gravity, 186; of water, 188, 190; fat available for butter in different grades of, 165; from cows in heat, 89; from sick cows, 89; from single cows, sampling of, 126; variations in, 116; frozen, sampling ot, 24; gases, 19; hypoxaothin, 19; lacto- cbrome, 19; lecithin, 19; mineral com- ponents, 17; partially churned, sampl- ing of, 21; quality of, influence of food, 131; of heavy grain feeding, 130; of pasture, 131; method of improving, 132; sampling, 20; scales, 124; serum, 11; skimming, 90; solids, 11; calcula- tion of, 'i^\ sour, sampling of, 23, 26; standards, 89, 206; sngar, 15; testing purity of, 111; urea, 19; watering of, 91; watering and skimming, 91. Milk test, a practical, need of, 1; re- quirements of, 6; bottle, use of. in testing cream, 69; Kussian, 62. Milk tests, Beimling (Lelfmann and Beam i 5; Cochran, 4; DeLaval butyrometer, 8; Failyer and Wil- lard, 4; Fjord^ 9; foreign, 7; Gerber acid-butyrometer, 8; introduction of. 3; lactocrite, 5; Licbermaun, 5, Par- sun, 4; Patrick (Iowa station te;-t.} 5; Roese-Gottliclj, 5; Schmied, Tv. short, 4; Thoerner, 5. Milk testing, 26; on the farm. ln;. N. Y. board of health lactomch-r, 83; degrees corresponding tu ^Mievt-nne lactometer degrees, 207. Non-fatty milk solids. 11. Normal solution.^, 96. "No-tin" test, 52, Nuclein, 14. Gil test churn, 2, 151, Olilsson tp.,t butile, 76. Oleomargarine, ilelection of. '.^nO; cheese, detection of. 203, One-third sani])ling pipette, use of, 138. Organization of co-operative creauieries and cheese factories, suggestiuns con- cerning, 229. Overrun, 163; calculation of. 167; faetors influencing, 163; t?b]e, 224; use of. 170. Parson's test, 4. Pasture, influence of, on quality of milk, 131 . Patrick's test . 5. Patron's dilemma, a, 146. Percentages, average, methuds of cal- culation, 145; fallacy of averaging, 144. Phenolphtalein, 97. Pipettes, 39; proper constrLictiun of points, 40, Potassium bi-chromate, 141. Power testers, 53. Preservaliue, 109; detection of, in milk. 109. Preservatives, for romposite samples, 140; in milk, detcetiun of, 196. Primost, 14. Proteose, 14. Quevenne lactometer, the, SO; degrees corresponding to scale ofN. V. board of health lactometer, 2o7. Record of tests of cows, 126. Reichert number, 202, Iteichert-Wollny method, 2ul. Tcstiuij- Milk and //s Products. >> Reintive value tables, 17K, 180, 2is. Reservoir fur water in Babcock teat, r,l. Roese-iiottiifb metboil, .1. Rus^iiiii milk test, the, fJ'J. iSalicylii' Jicid, in milk, detection of, SampliDg cheese, 77; niilk. 'JO. 2G; milk from single cows, 120. Schmied ruetliod, the, f>. Scovell yampliog tube, l-'iG. Serurn solids, 11. Short's test, 4. Skimming (jf milk, detection of, OD. Skimiiiilk, Babcock test for, 74; chemi- cal aualysis of, 197; composition of, L'J"; ti-st bottles. 71";, 77. Solids not fat, 11; foimulafor calculat- ing, S6; table showing, corresponding to 0-0 |ier cent, fat and 26-36 lacto- meter degrees, 20'J. Sour milk, sampling of, 23. Spice system, the, LjO. Specific gravity, 80; cylinders, 84; in- fluence of temperature, 81; of butter lat. determination of, 200: of milk, determination of, ISd; temperature correction table, 2ii8. Speed required for com|ilele separation of fat, 48 Spillmau'8 cylinder, VdT). Steam turbine testers, G3. Sulfuric acid,o4; table showing strength of, 56; testing strength of, 54. Swedish acid bottles. 42. Swedish acid tester, 57. Tank for cleaning test bottles, 40 Test botilep, 34; apparatus for clfianing, 38; cleaning, 86; douhle.-necked, 76; drain ing-rack for, 30; marking. 35; for cream testing. 07: for skim milk testing. 70, 77; rack for use in cream- eries and clieese lactorics, 130; tank for clesining, 40. Testers, hand, 52; p wer, 53. Testing cows, number of tests required duiing a period of lactation, 121 . Testing milk and its products, 1; on the farm, 116. Test sample, size of, 128. Tests of cows, record of, 126. Thermometer scales, comparison of. 220, Thoerner's method , 5. Total solids in milk,)]; determination of, 189. Volatile acids, 201. Waste acid jar, 37. "Water, calibration with. 45; determina- tion of, in butter, 107; in cheese, 202; in milk, is"?, 190; reservoir for, 61 ; to be used in the Babcock ttst, 00. Watering of milk, detection of, 91. Watering and skimming of milk, de- tection of. 91. Weights and measures, comparison of metric and customary, 228. Whey, Babcock lest for, 74, 7"; ch'-mical analysis of, 197; composition of, 205. Winton cream bottle, the, 69, Wisconsin creamery butter, summary of analyses, 102. Wisconsin curd test, the, 111. World's Fair breed tests, comji'isitioD of butter from, 101. Yield of butter, calculation of, 100. and butter fat test, 160; from milk of dif- ferent richness, 16^^; table showing, from 1 to 10,000 lbs. of milk, testing 3 to 5 35 per cent.. 222 Yield of cheese, cabulation of, 160, 171; relation between, and quality ofmilk^ 171; table sh'^'wing, corresponding to 2.5 (oii per cent, of fat, with laito- im-ler readings ol 2i; to ::6, 225.