• • • 111 tJt • • • Queen Acme No. 5 Microscope NEW MODEL n £ n. !^ E r" Co '-A 55 ^r^ ■— t 5Cf < 5 s: S > ^ '•y) ^^ »*— • '7 ACME No. 5 Outfit No. 3101 with % in. and ^ In. Objectives, one Ocular and Double Nose-piece. We cfaim that this instrument fills, as does no other at the price, the wants of the amateur and the teacher. In the smooth, precise and permanent action of its focal and other adjust- ments it stands unapproached. QUEEN & CO., Inc. NEW YORK OFFICE ■ 16 FULTON STREET loiQ Chestnut Street PHILADELPHIA CORNELL UNfVERSITY LIBRARY 3 1924 089 431 997 DATE DUE 1 GAYLORD PRINTED IN U.S A Trom the Estate of Bruce Voeller ANALYTICAL KEYS TO THE GENERA AND SPECIES Fresh Water Alg^ The iJesmidieae of the Tnited States, KOUNUEIJ l)N THE CLASSIFICATION OF THE REV. FRANCIS WOLI-E'S MONOGRAPHS, DR. ALFRED C. STOKES, Author of "Microscopy for Beginners;" "A Contribution toward a Natural Histor\' of the Fresh Water Infusoria of the Lfnited States," etc. PORTLAND, CONN.: ElJWARD F. BiGELOW, PUBLISHER. '893. ManM 51 I SSlx l^^3 Entered according to act of Congress, in the year iSpj, by E. F. BIGELOW, Publisher, in the office of the Librarian of Congress, at Washington. CONTENTS. PAGE. Explanation of Plate, 7 Value of of Mikrons in parts of an inch, 9 Introduction, 11 Key to the Classes of the Fresh-water AlgK, 25 Key to the Genera of the Fresh-water Algae, 25 Key to the Species of the Fresh-water Algae, 37 Key to the Genera of the Desmidiese, 78 Key to the Species of the Desmidiese, 80 Glossary, in Index, 115 Cornell University Library The original of this book is in the Cornell University Library. There are no known copyright restrictions in the United States on the use of the text. http://www.archive.org/details/cu31924089431997 Explanation of Plate. [FRONTISPIECE.] CxENERA OF DESMIDS. Each Figure is numbered to correspond with the number of the genus. FIG I 2 3 4. 5 6 9 lO II 12 13 14 15 16, 17 18, 19 20 21 22 23 24 Gonatozygon. Omitted; the cell needs no figure. Genicularia. Hyalotheca. Bambusina. Leptozosma. Omitted; the cell needs no figure. Desmidium. Phymatodocis. Sphaerozosma. Onychonema. Mesotsenium. Spirotaenia. Penium. Closterium; three figures, showing central inflation and ends produced into beaks. Docidium. Triploceras; end and part of cell. Calocylindrus. Cosmarium. Tetmemorus. Xanthidium. Arthrodesmus; 20a, front view, h, end view. Euastrum. Micrasterias; five forms. Staurastrum; four forms; 23a, front view, b, end. Pediastrum; three forms. Value of Microns (m) in fractions of an inch. /< Inches. /x Inches. 18.0=1-1383 /t Inches. 45-° = i-S33 /J. Inches. 79.0=1-316 /x Inches. 1.0=1-25000 113.0=1-221 1.5 = 1-16666 18.5 = 1-1351 46.0=1-543 80.0=1-312 114.0 = 1-219 2.0=1-12500 19.0=1-1315 47.0=1-532 81.0=1-308 115.0=1-217 2.5 = 1-10000 19.5 = 1-3333 48.0=1-521 82.0=1-304 116.0=1-215 3.0 = 1-8333 20.0=1-1250 49.0=1-510 83.0=1-301 117.0=1-213 3.5 = 1-7222 20.5 = 1-1214 50.0=1-500 84.0=1-297 118.0=1-211 4.0 = 1-6250 21.0=1-1143 51.0=1-490 85.0=1-295 119.0=1-209 4-5 = 1-5555 21.5 = 1-1162 52.0=1-480 86.0=1-291 120.0=1-208 5.0 = 1-5000 22.0 = 1-1131 53.0=1-471 87.0=1-287 121.0=1-206 5-5 = 1-4545 22.5 = 1-1111 54.0=1-464 88.0=1-284 122.0=1-205 6-0=1-4333 23.0=1-1087 55-0=1-454 89.0=1-281 123.0=1-203 6.5 = 1-3846 23.5 = 1-1064 56.0 = 1-446 90.0=1-277 124.0=1-201 7.0=1-3285 24.0=1-1042 57.0=1-438 91.0 = 1-274 125.0^1-200 7-5 = 1-3333 24.5 = 1-1020 58.0 = 1-431 92.0 = 1-271 126.0=1-198 8.0=1-3125 25.0=1-1000 59.0=1-423 93.0=1-268 127.0=1-196 8.5 = 1-2941 26.0^1-961 60.0=1-416 94.0 = 1-266 128.0=1-195 9.0=1-2777 27.0=1-926 61.0=1-409 95.0=1-263 129.0=1-193 9.5=1-2631 28.0 = 1-893 62.0 = 1-403 96.0^1-260 130.0=1-192 10.0 = 1-2500 29.0 = 1-862 63.0=1-396 97.0=1-259 131.0=1-191 10.5 = 1-2381 30.0=1-833 64.0=1-390 98.0=1-255 132.0=1-189 11.0=1-2272 31.0 = 1-806 65-0 = 1-384 99.0=1-252 133-0=1-188 11.5 = 1-2173 32.0=1-781 66.0=1-378 100.0=1-250 134.0=1-186 12.0=1-2166 33.0=1-787 67-0=1 373 101.0=1-247 135.0=1-185 12.5 = 1-2000 34.0=1-706 68.0=1-368 102.0=1-245 136.0=1-183 13.0=1-1923 35.0=1-714 69.0=1-390 103.0=1-242 137.0=1-182 13.5 = 1-1851 36.0=1-694 70-0=1-357 104.0=1-240 138.0=1-181 14.0=1-1785 37.0 = 1-675 71-0=1-352 105.0=1-238 139.0 = 1-179 14-5 = 1-1724 38.0=1-657 72.0=1-345 106.0 = 1-235 140.0=1-178 15.0 = 1-1666 39.0 = 1-641 73.0 = 1-342 107.0=1-233 150.0 = 1-166 15-5 = 1-1612 40.0 = 1-625 74-0 = 1-337 108.0=1-231 175-0=1-142 16.0=1-1562 41.0=1-609 75-0 = 1-333 109.0=1-228 200.0^1-125 16.5 = 1-1515 42.0=1-593 76.0=1-328 1 10.0^1-227 17.0=1-1470 43-0=1-583 77.0=1-324 111.0 = 1-225 17.5 = 1-1421 44.0 = 1-567 78.0=1-320 112.0=1-223 INTRODUCTION. To know the origin of any object, event or act, is always of in- terest. To know what lead up to a certain acceptable innovation or aid is pleasing to everyone, and often important to the historian of any department of the world's work. What was the form of the first analytical key, who made it and what induced him to make it, are in- teresting questions that the writer at least cannot answer. Such tables have become very popular in the various departments of bot- any and of zoology, but who originated them is not known; nor when the first was published, nor where. Like many other good things, these keys may have been the result of simultaneous thoug'ht and experiment on the part of several workers that felt the need of some outside aid to the inside treasures of the ever increasing mass of scientific knowledge. But to whomsoever belongs the credit of or- iginating these devices, to him belong much praise and many hearty thanks. That analytical keys were needed is proved by their popularity. They are welcomed by the student in all the sections into which Na- ture is for convenience divided. It is true that there are some ob- servers who scoff and laugh in derision when "keys'" are mentioned, but these few belong to that class, fortunately limited, whose mem- bers are unable to use them. There are persons that seem to be de- ficient in those mental qualities that make the use of such tables a pleasure and a delight, as there are others unable to arrange even the simplest of keys. If in a scheme of the kind there are three groups, a blue, a black and a green, these unfortunate opponents of keys seem unable to decide what to do next, when the specimen hap- pens to be green while the first description in the list calls for a blue 12 INTRODUCTION. object. That they should glance down the list to see if by any chance a green specimen may have been provided for, does not oc- cur to them; they toss the paper aside, and laugh at the utter folly of the statement that any intelligent person should be able to use a key. ■ The truth is that inability to use these valuable aids is an evi- dence of a defect in the mental organization. Such critics are forced to page through the monographs they attempt to study, with great loss of time and with much labor, to find the information that may be wanted; whereas with the object and a key to its class in hand the labor becomes a delight, and the result a valuable acquisition, be- cause no key to any department can be used even to a limited extent without teaching the student many facts that he might otherwise have overlooked. A table of the kind will draw attention to the morphology of the object in a way that will lead not only to its proper classification, but to something much more important, the enlarging of the stu- dent's powers of observation. Some of those that oppose the use of artificial keys are themselves compelled not only to page through an unfamiliar monograph, but in many instances to scan the pictures for the light they are groping after; and failing pictures they guess as to the character of the object from its resemblance to something re- motely similar, and in the end, at great expense of time, and perhaps of temper, may get a clue that may lead them to the goal they have been so laboriously seeking. With the intelligent use of an artificial key the place, the character, and even so unimportant a matter as the name, can be rapidly obtained, and the student be led on to fur- ther study and investigation. It is always encouraging to feel that by one's efforts a way has been opened to further conquests, or even that a path has been cut to some previously unknown view. This the use of artificial keys always accomplishes. When the beginner feels that he can do some- thing unaided he is inspired to do more. When he feels that he has travelled alone through soine pathway in some department of natural science, he is anxious to go further. One step taken with the aid of an artificial key is sure to be an encouragement toward another. The powers of observation are increased. The ability to balance one thing against another is cultivated. In a word, an artificial key can be made one of the most important aids in the mental development of the beginner that can be imagined. The ob- server that scoffs at these aids, the observer that refuses to take advantage of them, is doing a foolish thing and losing a good one. INTRODUCTION. 13 There are two distinct varieties of these valuable aids, the nat- ural and the artificial. The former is founded only on the charac- ters that the advanced scientist uses in his classification. These are often so exceedingly natural that to learn the proper position of the object it must be destroyed. To learn something of an animal through them the animal must be dissected, and the anatomy of its nervous system, and the morphology of its osseous system, and the structure of its heart be accurately observed, before it can be classed. These arrangements may be, they often are exceedingly valuable to the advanced scientist; to the amateur and the beginner they are terrible. They haunt his dreams like horrid night-mares, provided he is incautious enough, or ignorant enough to attempt to use them-. I have not a word to say against such analytical tables. I commend them for use in their proper places. They are important for their purpose, but that purpose cannot be the beginner's or the amateur's. It is the learned man that can delight his soul with such a scientific key. To him it is instructive and helpful. To any one else it is a bugbear and a horror. The beginner is not prepared to begin with the nervous system of his "find," oreven with the structure of its heart. He must have something nearer the surface and more easily seen than are these parts. His key must deal with the external and the evident characters. It is for him that the artificial key is in- tended, and to him it is inspiring and helpful. This second kind of key, this artificial kind, is as its name indi- cates, founded not on what the advanced investigator would use in his classification of the animal or the plant, but on some obvious, prefer- ably external, points that may be of no use in the art of classification, but that can be used as crutches to help the student over the miry and the stony ground to the hills of science where he will be able to look back and smile at his former helplessness. These artificial keys have this for their object, and only this. They aim to help over the hard places; to encourage the user to go further, and to do more, so that finally he may become an expert, when he will no longer need any but the natural aids, or may even get along without any other assistance than skilled eyes and a "learned touch." The beginner, however, must have a guide, especially if he be trying to work alone. This is true in every department of natural science. It is especially true in microscopy. The field is so im- mense, the outlook so vast, the work so apparently endless, that with- out an aid the beginner at once begins to flounder in his sea of troubles, and before long he sinks discouraged to rise no more. He 14 INTRODUCTION. becomes so disheartened since everything is so new and strange, and there seems to be no one within call to lead him out of the difficult- ies, or out of the embarrassment of scientific riches, that he is entirely discouraged, his enthusiasm is lost, and with this loss is abandoned a study that would have lessened his cares, increased his joy in living and lengthened his life. The beginner working alone wastes much time. To be sure, he may before long learn that a green object that stands still is a plant, or may be; and also before long he hears that all green microscopic objects that stand still are not always plants. If he have a teacher his condition is a more favorable one; but if he be alone he must have an artificial guide, or fail. For such students the artificial keys are providential. Once introduced to them he is ready to go on to new conquests. The use of such tables in the popular treatises on botany has done more to popularize that beautiful study than a score of learned monographs on the subject. As soon as the pupil learns that he can by his own efforts' obtain even the bo- tanical name of his plant, a feeling of enthusiasm fills his heart, and he is eager for another specimen to analyse. And in the analysis he is learning the principles of classification, the structure of the specimen and the function of the various organs. By means of the key he goes with little trouble and waste of time to the order, there he finds another key that leads him pleasantly to the genus, and perhaps a third that takes him to the species. There he finds the plant exactly described. He has had the prominent characters brought to his no- tice in a pleasing way, and the final result is that he not only learns to love the science of botany, but that he soon learns to know at a glance, without the use of the artificial key, to what order a strange specimen may belong. He has become scientific without knowing how he became so. He has travelled by the royal road. It is so, or should be so, in the science of microscopy, to call for convenience a science that which is really a combination of all the sciences. If every department of microscopy could have an artificial key to open the doors of its treasure-houses, the amateur would have a hap- pier time, science would be benefitted and the maker of the key, the scientific artisan, would himself be blessed both in this world and in the next. Such a pleasing state of affairs can not be attained. The doors are too numerous to be opened by a single key. To make the proper number is not possible for one worker, however willing he may be to try. Each specialist could forge a key to the doors or to the dark passage-ways of his- special scientific castle, and offer it to those that would follow after him if they could. But the specialists are not INTRODUCTION. 15 disposed to become scientific blacksmiths, and to forge keys to the treasures they have locked up in their formidable monographs. In- deed, it is not possible that they should. They have no time to make the entrance ways easy and agreeable. They are after the facts of the science; they are seeking the internal secrets of the creatures they have studied and written about; they are content to leave the en- larging of the building to other specialists that may come after. It is no fault of the writers of learned monographs that they put forth their treatises without a single loop-hole of entrance for those that would, if they could, enter in to see some of the treasures of which rumor has told. When a man has spent his days and his nights and his earthly and bodily substance on the production of a treatise that shall make his name known to the world, he is naturally disin- clined to labor longer and harder to make easy grades and smooth paths and shady retreats along the way to his treasure house; and when the learner has 'arrived at the gates, the learned man is not dis- posed to stop his further investigations to throw a pretty key out of the window and ask the uninvited guest to come m and sup with him. He is too busily engaged in the new investigation that his completed investigations have made necessary. There is no end to the questions to be studied and decided. He cannot, much as he may be willing condescend to make keys for the beginners. If he makes any at all, they will be of the severest kind and intended for his scientific equals only, not for the humble followers in his foot-steps. We should never blame the writers of learned monographs for not offering these gilded keys. They cannot. They have gone up too high to stop for the higher they go the more there is to be done. And they that have climbed that high are the ones best adapted to climb higher, for they find it easier to go up than to come down. But if such books are to be used by any others than the rather limited class of experts that have the knowledge needed to find their way unaided through the pages, then a guide of some kind must be furnished. An index will not answer the purpose, as to use it de- mands just that information not at the disposal of the novice orof the amateur. All of these scientific treatises may be as useful to the modest student as to the learned investigator, provided, as I have so often said and repeated, some means can be devised by which he may be helped to make an intelligent entrance into its various depart- ments. Mr. Wolle's monographs on the Algse and the Desmids are no exception to the rule. They are not adapted to the use of the ama- l6 INTRODUCTION. teur microscopist in the state in which the author has left them. It is not possible for the youthful microscopist to read the description of one hundred and twenty genera of Algse in order to locate a single specimen that he may find in the wayside ditch. To ascertain with any degree of comfort the probable resting place of the speci- men he must have some help. It is that help that this little compila- tion hopes to supply. By its intelligent use the student may ascer- tain the specific name of any fresh water Alga or Desmid in the ponds and the ditches of the United States. This he can do with the ex- penditure of the least time and labor, leaving them to be spent in the subsequent study of the plant, after he has had the satisfaction to learn what it is, and where in the classification it belongs. If the worker has the name of the specimen, he then feels that he has a sup- port on which he may hang his subsequent information, and a starting point for further investigations. Not to know the name of the ob- ject and yet to try to study it, is like an attempted conversation with a stranger whose antecedents and local habitation are unknown. The effort is not an agreeable one. The conversation languishes, and interest is lacking. The name and the habitat of the person add much to the pleasure of the meeting, and many suggestions to the in- terview. So the name of the microscopical or other specimen is al- most essential to the well being of the young student. The name is, too, about the first thing the advanced scientist tries to learn. It is impossible to do otherwise.' The object can not be re- ferred to by speech or in writing until its name is known; what other workers in other parts of the world may have said about it or done with it, can not be known until its name is learned, as without the name all indexes are closed in all the books of all the libraries. The name is the clue to further knowledge, its starting point, even the hook upon which further information is to be hung. Whatever advanced scien- tists may say to the contrary, their first effort, perhaps it is an uncon- scious one, but their first real effort is to ascertain the name of their new specimen. If it has none, they at once proceed to give it one. All the wild talk about the un desirability of learning the name is wrong in principle. The name is, as every one will cheerfully admit, only of secondary importance when compared with a study of habits or of morphology, but it is as essential, since it is and must ever be the starting point for further investigations, at least on the part of the amateur. If he chooses to stop at the name, that is his misfortune; but even then he has gained something. If those whom I may be able to help by these keys shall do nothing more than identify their INTRODUCTION. 17 "finds," I shall feel that I have done some little thing to help them and their friends. I shall feel pretty sure that the mere finding of the name will not be the end; but if it should be, where will be the harm ? Is it a crime to know the scientific name of the plant or of the animal ? Knowing that is knowing something, I think. Would you have the pupil dissect the nervous system before he has learned to recognize a nerve, or before he knows the muscles to which the nerves are distributed ? Of course he might say "This is a nerve," and "That is a nerve and it goes to that muscle," but would you have him carry the dead body about with him in order to dissect it and point out the nerves and the muscles that receive them whenever he wished to com- municate with you or with any of his friends in refere'nce to the nerves and the muscles of that special creature ? This is exactly the result which those that cry out against learning names would accomplish if their desires prevailed. No, no ! Get the name the very first thing, if you wish. It will make a good starting point. It is not the end and the aim of any study; it is only the bridge that takes us over the swamp. After awhile we will be big enough and strong enough to pass the swamp without the bridge, as after awhile we will be able to get along without an artificial key. But do not condemn the bridge that has carried you safely over. The keys in this little book are as artificial as they could be made. In some instances they are perforce somewhat natural; that is, the fruiting plant has been used to the exclusion of the more frequent sterile condition. This, however, has been avoided as much as possi- ble, and in several instances two keys have been prepared to the same genus of plants, one for the fertile, the other for the sterile filaments. They are all, as the title page indicates, founded on the classification contained in the admirable monographs by the Reverend Francis Wolle on the fresh water Algae and the Desmids of this country. Un- til Mr. Wolle took up the subject our American microscopic plants had scarcely been touched, except by a few observers in a desultory way. He has brought order out of chaos, and has prepared two mon- ographs that are monuments to his learning, skill, care and patience. These keys are made not alone with the object of leading the begin- ner to the study of the fresh water Algae and the Desmids, but to in- troduce him to these fine works of Mr. WoUe's. While the beginning mcroscopist in his study of microscopical botany may, to a certain ex- tent, succeed in identifying his "finds" with these keys alone, to do so with entire satisfaction he should have access to the monographs on the Algae and on the Desmids. The references at the end of the 18 INTRODUCTION. classes and of the genera are to the numbers preceeding those classes and genera in this list; those following the species are in every case for reference to the pages on which will be found the extended de- scriptions in the monographs already so often referred to, where ref- erences will also be found to the illustrations. The measurements of the Algse are given in parts of amikron (/i) ; in the Desmids, by both fractions of an inch and of mikrons. The former will necessitate a little calculation, if the reader is not en- tirely familiar with the minute space called a mikron and represented by the Greek letter fA.. It measures the ^g^^ ^, of an inch in length, and the calculation needed to bring it to the fractions of an inch as given in the measurements of the Algse, are slight. Twenty-five mikrons, for instance, being g g^^, ;, inch, or -j-ifW inch. It is only necessary therefore to use the number of mikrons as given with the Algae for the numerator of a fraction of which the 25000 forms the denomi- nator, and reducing the whole to the lowest terms. In the Desmids the calculations have been made, and the measurements given in both forms. The student will therefore need an eye-piece micrometer, but it may be ruled to parts of an inch. The use of the mikron, like the use of the metric system, has not and probably never will come into popular favor. Does the reader know how to use a key of this kind ? To some this appears to be a mysterious thing, although a little inspection of the key itself should make it plain. It is simply a matter of exami- nation, comparison and rejection. ' With the specimen in hand, which in this instance means under the microscope, begin at the beginning of the key and compare the description in the first sentence with the object under the instrument. Do the two agree in every particular? If not, leave that reference and go to the next having the same letter or other symbol at its front. If that should describe the specimen, notice the letter in the parenthesis at the end of the line, and seek that same letter at the beginning of a line in some other part of the key. You will probably find several lines beginning with that letter. Starting at the first, compare the description there given in a word or two with the actual specimen, and if it does not agree pass to the line next below. If at the end of this line there is another reference let- ter in parenthesis, turn to that, and so continue until at the end of some line you find the generic or the specific name of the plant. Mis- takes are easily made, here as elsewhere. If you should happen to make one at the start every step forward will lead you further and further astray. With care and intelligent observation, errors may be INTRODUCTION. I9 avoided and the specimen readily run down to its proper place. Af- ter a little practice, the use of such artificial keys becomes a great pleasure. The benefit has already been referred to. The only secret of their successful use is to be certain that each progressive step is right before it is abandoned for the next. And after a little practice, too, the student will become so familiar with the Algae or with the Desmids that he will not need to apply to the generic keys for aid, but will be able to turn at once to the proper genus, and there use the keys to the species; and with a little further experience he will be en- abled to recognize at a glance the species, and then will be prepared to proceed with his investigations, or to refer intelligently to the ob- ject so as to communicate with his friends and fellow workers in re- gard to it. It is as impossible to speak or to write of an Alga or of a Desmid without using its proper name, as it is to speak or to write of your most intimate friend without using his. AlgK and Desmids are singly invisible to the naked eye. It is only when they occur in large masses that the eye can take cogni- zance of them. It rarely occurs, however, that the Desmids are so abundantly congregated that they thus obtrude themselves on the observer. When a large quantity has been collected and the vessel placed near a window, they will collect in a green film at the surface of the water on the lighted side, and there become visible in mass. In the ponds and shallows such an occurrence is not common. At times they are found so abundantly that by holding a glass vessel of the water up to the light they may be seen floating about as minute green objects, which the trained eye will recognize and the pocket lens make distinct. But these varieties are among the largest of the forms; according to my experience they are always exclusively con- fined to the Closteriums. Other large forms, like Micrasterias, at least in the writer's locality, rarely occur in such profusion. To col- lect the Desmids, therefore, it is necessary to collect by faith. The microscopist can know exactly what he has only when he gets home and examines the water drop by drop under the microscope. With the Algae it is different. These are usually visible to the naked eye, as they are almost invariably collected in large masses floating on the surface, submerged just beneath the surface, or at- tached in waving tufts or fringes to sticks and stones and other plants in the ponds. The eye of faith is not needed to recognize them. They usually force themselves on the wondering attention of the observing pedestrian in the wayside lanes, beside the ditches and slow brooks. As soft emerald clouds, or graceful streamers floating^ 20 INTRODUCTION. in the sluggisji current, or resting like a green slcum on tiie surface, they are readily seen and as easily gathered. No collecting tools are demanded for either Algse or Desmids, except a dipper of some kind, a common tin dipper is as good as anything, and a few bottles or other vessels to carry the treasures home, and to keep them con- cealed from inquisitive people; the collecting naturalist must always be prepared for a meeting with such persons. Many media have been recommended for the preservation of these beautiful plants. The trouble with the majority of these prepara- tions is that they will in time, some usually in a very short time, allow the cell contents to contract and to become so distorted that the object is worthless for any serious study, and worthless, too, as the object of beauty that it was when first mounted. Some of the commended media act well on some kinds of Algae and Desmids, but fail on others. The following are among the best, yet even these are not all adapted to all the different kinds. They are mentioned in the order of their excellence. CAMPHOR WATER. — This is made by placing a lump of camphor in distilled water and leaving it there for several days. Mr. W. H. Walmsley, an expert preparer of microscopical slides, says that he has had a specimen of Draparnaldia in camphor water for twenty years, and that it is now as beautifully green as when first mounted,' and that the chlorophyll, its green coloring matter, seems to be un- changed. When he desires to mount an Alga or a Desmid, he places it in a small quantity of camphor water to which a few drops of glyc- erine have been added. At first the plant will become lemon color, but after a few hours the original green returns in all its vividness, and then he at once mounts the plant in some of the fluid. CARBOLIC ACID WATER, made by adding a few drops of the acid to a phial of water, is recommended by the Rev. Mr. WoUe, but he says that although this will preserve them for months or even years without deterioration, it may allow the coloring matter, the chlor- ophyll, to fade; this however in the case of the Desmids is of little importance. A SOLUTION OF CHLORAL HYDRATE, in the proportion of five grains of the salt to one ounce of water, answers well for some specimens. Like camphor water it has the merit of being easily made and easily used. A SOLUTION OF COPPER made after the following receipt is admir- able for some of the Algs, preserving the color and the form entirely unchanged, while on others it will not act so favorablj'. INTRODUCTION. 21 Camphor water, - 50 grammes; Distilled water, 50 grammes; Glacial acetic acid, - 0.5 grammes; Crystallized chloride of copper, 2 grammes; Crystallized nitrate of copper, 2 grammes. Dissolve and filter. When mounting with this copper solution the cell should be made and the cover cemented down with shellac. With almost any other cement failure is pretty certain, as the cement will surely run under and ruin the preparation. After the shellac is hard, it is a good plan to add some of Brown's rubber cement around the cover. This is entirely transparent and will make the mount more secure, and at the same time add something to the beauty of the finish. TRENTON, N. J., 1892. PART I. Key to the Classes and the Genera of the Fresh- Water Algae. KEY TO THE CLASSES AND THE GENERA OF THE FRESH WATER AI.GJE. CLASSES OF THE ALG^. § Rosy red, blood-red, reddish-brown, purple or blackish; many- celled . . . . . CLASS I. § Chlorophyll-green, sometimes becoming crimson, purplish, flesh- color or yellow-brown; 1,2 or many celled; often nucleated, CLASS II. § Never chlorophyll-green; colorless or variously colored; i or many celled; in jelly when out of the water . . . . class iii. KEY TO THE GENERA OF THE ALG^. CLASS I. ^ Olive, brown or grey, black with age; filamentous, often hollow, bristle-like, rigid (A). ' Tf Violet, violet-purple or bluish-green; filaments articulate, branched or not (E). T[ Purple; tufts loose; filaments i in. long, not branched (D). T[ Purplish, smooth, forming a firmly attached coating to stones, Hildebrantia, 7 *{ Brownish red, Vermillion, or greenish with red centre; cells 4-8, free or in a short lived cluster Chlamydococcus, 38 *{ Blood red, cells large, tegument thick, lamellose, stem ringed, Urococcus, 66 A. Filaments nodose, tufted; fertile filaments hollow, with internal axillary thread; in rapid water (B). ■A. Filaments transversely banded or annularly constricted; with in- ternal central axis (C). 26 GENERA OF ALG^. B. Axillary thread a single series of cells with transverse threads at regular intervals .... Lemanea, i C. Axillary thread of cohering filaments, with many whorls of mon- iliform branchlets . . . ... Tuomeya, 2 D. Simple or branched, slippery; cells in a single layer, membrane thick, colorless ... . ... Bangia, 3 E. Branched; main stem often with an external, parallel series of cells; branches moniliform, clustered . . Batrachospermum, 4 E. Branched; main stem without external parallel series of cells; branches not moniliform (F). F. Filaments articulate, villose, branchlets dichotomous . Thorea, 5 F. Filaments articulate; branches not whorled; plant steel blue or purplish .... . Chantransia, 6 F. Filaments articulate; branches erect-spreading; plant olive-green, Compsopogon, 8 CLASS II. § Multicellular or apparently so (A). § Unicellular, the cells often clustered or forming a colony (F). A. Chlorophyll diffused, not in patterns (B). A. Chlorophyll in spiral bands, axillary laminae, or twin, stellate nuclei {p). B. Filamentous (C). B. Not filamenious; articulate, prostrate, or net-like, often membra- nous, sometimes parasitic (M). C. Basal cell lobately divided, or with a terminal disk attaching the young plant (D). C. Basal cell not lobate nor disciform (E). D. Not branched; terminal cell sometimes setiform, CEdogonium, 10 D. Not branched; no setae; strongly contracted at intervals, Schizomeris, 24a D. Branched; with long terminal setae bulbous at base, BulbochcBte, 11 D. Branched; no setae; cells long, cylindrical . . . Sciadium, 46 E. With branches (G). E. Without branches, but sometimes with lateral processes (L). F. Plants filamentous (N). F. Plants not filamentous, sometimes curved or spiral, without root- lets (T). F. Plants globose, small, on moist earth, with colorless rootlets (O). G. Filaments erect (H). G. Filaments prostrate or creeping, branched or not (*). GENERA OF ALG^. 27 * Without dorsal spine; cell walls thick . . . Gongrosira, 21 * With dorsal spine . . Aphanochmte, 20 H. Immersed in firm jelly ... . . . Chcetophora, 18 H. Not in a firm jelly (I). I. Filanjents dichotomously or trichotomously branched, Microthamnion, 1 9 I. Filaments not dichotomously branched (J). J. Branches in lateral clusters; cells of main stem with a transverse, central chlorophyll band . . . Draparnaldia, 16 J. Branches not in lateral clusters (K). K. Cell membrane usually thick, cells longer than broad; ultimate branches, much thinner than the primary, Cladophora, 23, or Pithophora, 24 K. Cell membrane very thin and hyaline; stem and branches barely separably in size and appearance . Stigeodonium, 17 K. Cell membrane thin; branches radiating, umbel-like, one-celled, Sciadium, 46 L. Distinctly many celled (P). L. Articulate (apparently multicellular) (Q). M. Articulate, branched, forming a little mass or a subdisciform layer ..... .... Coleochmte, 9 M. Articulate, prostrate or creeping, a spine on the back, Aphanochxte, 20 M. Expanded, leaf-like, smooth, formed of angular ceils; always ad- herent . . . . . . Prasiola, 14 M. Membranaceous, tubular or utricular, fixed only when young, Enteromorpha, 15 M. Forming a green net, visible to the naked eye, Hydrodictyon, 41 M. Forming a colony, plane; discoid or stellate, often perforated; cells polygonal . . . . Pediastrum, 40 N. Filaments tufted, long, sometimes branched, . . Vat/cheria, 30 O. Rootlets much divided, descending from the cell base into the earth . . Botiydium, 31 P. Cells long; chlorophyll enclosing large, regularly placed vacuoles, Spharoplea, 1 2 P. Cells long, filaments somewhat geniculate, without vacuoles (f). P. Cells short, cylindrical or oblong; without vacuoles; filaments not contracted; attached when young .... Cylindrocapsa, 13 P. Cells short, broader than long, filaments contracted irregularly, Schizomeris, 24a Q. Filaments simple, not laterally united, (R). 28 GENERA OF ALG^. Q. Filaments 2 or more laterally united, or in flat narrow bands, Schizogonium, 26 R. With one row of cells, (S). S. Without lateral processes (except Ulothrix rivularis), strictly un- branched . . . . .... Ulothrix, 25; Conferva, 27 S. With short, irregular, lateral processes, 1-3 or more celled, Rhizodonium, 29 T. Parasitic, i. e. penetrating the membrane of the host {g). T. Not parasitic (*). * Cells united or clustered in families (U). * Cells scattered, independent or irregularly clustered (Z). U. Colonies motile, each cell with 2 cilia (V). U. Colonies often free but not spontaneously motile, without cilia (c). V. Colony (cKnobiuni) spherical or circular (W). V. Colony flat, cells 4-16, angles rounded, in a colorless sheath, Goniuin, 36 W. No gelatinous coating; cells many on a hollow globe Volvox, 32 W. With gelatinous coating (X). X. Colony ovate or spherical (Y). X. Colony of 8 cells at equal distances around a circle, Stephanosphxra, 37 Y. Cells 16-32, globose, at regular intervals on a colorless sphere, Eudorina, 33 Y. Cells 8-16-32-64, globose, crowded, often angular from pressure, Pandorina, 34 Z. Cells free-swimming (a). Z. Cells attached to aerial or to submerged objects (1^). a. Ovate, granular, in a hyaline, obtuse sheath; contractile vesicle present .... Chlamydomonas, 39 a. Cylindrical, straight or curved, ends sometimes spinous, Ophiocytium, 47 a. Spherical, 3-15/t in diameter . Protococcus, 49 a. Spherical, 100-150/* in diameter EremosphcBra, 65 a. Compressed, 3-4-8 angled, angles often produced or bifid, Polyedriuni, 50 b. Aquatic; form variable, cells not spinous, usually stipitate, Characium, 48 b. Aquatic; form variable, cells often with a dorsal spine, Aphanochcete, 20 b. Aerial; cells circular, flat, numerous, becoming red by exposure, Protococcus, 49 GENERA OF ALGVE. 2^ b. Aerial, on moist objects; gelatinous, cells globose or angular, be- coming red .... . Porphyridium, 57 c. Family in jelly mass, thallus pyriform, irregular, tubular, clustered, branched, etc. (/). c. Family globose {d). c. Family plane, discoid or stellate, often perforated; cells polygonal, Pediastrum, 40 c. Family cubical, cells 4-8-16, rhomboidal . . Staurogenia, 44 c. Family of laterally united, often spinous or crescentic cells (e). c. Family of cylindrical cells (/). d. Cells in a single stratum; ccenobium hollow . . Cxlastrum, 42 d. Cells 4-8-16-32, wedge-shaped, apices sinuate or bifid; ccenobium solid Sorastrum, 43 d. Cells 2-4-8, globose or oblong . . Glmocystis, 60 e. Cells cylindrical to ovate, ends often spinous . Scenedesmus, 45 e. Cells not spinous, fusiform, cylindrical, straight or variously curv- ed, single or united .... .... Rhaphidium, 62 /. Upper cells spreading, contracted at base . . . Sciadium, 46 g. Cells globose or pear-shaped (h). h. Neck not cylindrical, aperture with a lid . Ckytridium, 68 h. Neck cylindrical, elongated . Olpidium, 69 i. Cells connected by fine filaments (*). /. Cells not connected by filaments (7). * Filament attached to the convex side of the cells, Dimorphococcus, 63 * Filament attached to the concave side of the cells, Dictyospharium, 51 j. Families free-swimmirig (not attached), (k). j. Families not free-swimming (attached), (/). k. Cells oblong, reniform, 2-4-8-16; tegument oval or reniform; variable ... Nephrocytium, 61 k. Cells oval, small, in grape-like clusters; tegument thin, diffluent, Botrycoccus, 59 k. Cells globose, large, cell walls firm, border hyaline; chlorophyll sometimes radiate . . . .... Eremosphcera, 65 /. Cells fusiform, crescentic or straight, cylindrical, ends cuspidate or acuminate, tegument thin . ... Rhaphidium, 62 /. Cells globose, elliptical or elongate (m). m. Cells red, large; tegument thick, concentrically lamellate, stem ringed . ... Urococcus, 66 m. Cells green; tegument not lamellate («). 30 GENERA OF AhGJE. n. Thallus soon diffluent into shapeless mucus, Tetraspora, 54, or Palmella, 56 n. Thallus with a definite form (0). o. Cylindrical or compressed, branched, 2-12 inches long; one pole of cells colorless Hy drums, 52 o. Cylindrical, sometimes septate, attached at one end and spreading, Palmodactylon, 53 o. Pear-shaped, fixed by the stem-like base .... Apiocystis, 76 p. Chlorophyll in spiral bands (ci). p. Chlorophyll in two, many-rayed bodies in each cell (f). p. Chlorophyll in axile plates (/). q. Conjugation geniculate. Gulf States .... Sirogonium, 71 q. Conjugation ladder-like Spirogyra, 70 r. Conjugation ladder-like or lateral (s). s. Spore in one of the cells Zygnemd, 72 s. Spore in the connective between the cells . . . Zygogonium, 73 /. Conjugation ladder-like {u). t. Conjugation lateral (v). t. Conjugation geniculate (w). t. Conjugation absent, spore formed spontaneously in the cell, Gonatonema, 78 u. Spore in the connective between the cells, Mougeotia,i/^, or Mesocarpus, 75 u. Spores in one of the cells Plagiospermum, 77 V. Spore lateral, at the point of two cells . . . Pleurocarpus, 76 w. Spore formed at point of conjugation . . . Craterospermum, 80 w. Spore between the cells, quadrangular in front view, Siaurospermum, 79 CLASS III. § Multicellular or apparently so, filamentous (A). § Unicellular, the cells sometimes clustered or in families (V). A. With hair-like points (B). A. Without " " (C). B. Branched (but spuriously so) ; in small tufts . Calothrix, 8 1 B. Not branched; not in jelly mass; heterocysts yellow, at the base of the filaments; free or in small mats . . . Mastigonema, 82 B. Not branched; in more or less firm mucilage or jelly (h). C. Branched; in a sheath or jelly mass (E). C. Branched; not in jelly. C. Not branched (D). GENERA OF ALGiE. 3! D. In a sheath or a jelly mass (K). D. Not sheathed nor in jelly (P). E. Branched at almost right angles to the stem; cells in a single se- ries .... .... ... Hapalosiphon, 92 E. Branched irregularly or with twin branches (F). F. Cells in single series in young plants, several series when older, Sirosiphon, 91 F. Cells in a single series only (G). G. Sheath broad, striate, hyaline, wing-like . . Petalonema, 90 G. Sheath not wing-like (H). H. Sheaths distinct, not agglutinated to one another (I). H. Sheaths agglutinated in erect, wick-like bundles, Symphyosiphon, 87 I. Heterocysts present (J). I. " not present; sheath with a single bluish filament, Plectonema, 8g J. Heterocysts disconnected from the twin branches, Scytonema^ 86 J. Heterocysts near the usually single branches . Tolypothrix, 88 K. In a sheath; filaments moniliform, curved . . Nostoc, 93 K. In a sheath; filaments not moniliform (Q). K. In mucilage or jelly mass (L). L. Jelly mass spherical; filaments transversely plicate, radiate, Glxotrichia 84 L. Jelly mass hemispherical, filaments radiate . Rivularia, 85 L. Jelly mass a stratum or indefinite (M). M. Filaments erect, attached at base, jelly stratum flat, often incrusted . . . Isactis, 83 M. Filaments not attached at base, not moniliform, heterocysts ter- minal, single . . ... . Cylindrospermum, 98 M. Filaments not attached, not moniliform, colorless, heterocysts none . . ... Crenothrix, 99 M. Filaments moniliform, cells globose or elliptical (N). M. " " cells compressed disciform (O). N. Heterocysts intercalated in the filaments, Anabcsna, 94, or Spharozyga, 95 O. Heterocysts intercalated at regular intervals, yellow, Modularia, 97 P. Bluish, bluish-green, blackish (T). P. Whitish or silvery; in sulphur waters . . . Beggiatoa, 105 Q. Two or more filaments in each sheath . . Microcoleus, 103 Q. -One filament only in each sheath (R). 32 GENERA OF ALG^. R. Parasitic; sheaths thin; cells short, tubular . Chamasiphon, loo R. Not parasitic (S). S. Agglutinate'd in erect, wick-like clusters, articulate, base prostrate, Symploca, 102 S. Not agglutinated; exceptionally appearing branched; often form- ing a stratum . . Lyngbya, loi T. Filaments parallel in dense clusters; free-swimming, Aphanizomenon, 96 ' T. Filaments short, heterocysts at their base; not free-swimming, Masiigonema, 82 T. Filaments slowly motile Oscillaria, 104 T. Filaments not motile (U). U. Filaments very slender, articulations more or less indistinct, Leptothrix, 106 U. Filaments cruciformly branched Asierothrix, io6a V. Free-swimming, (/. e. not adherent), (W). V. Not free-smimming (X). W. Cells globose, or oblong when dividing, in a single, quadrate layer, bluish-green ... . ... Merismopedia, 112 W. Cells globose, sometimes in a small colony, brownish or red, sometimes greenish . . Clilamydococcus, 38 W. Cells wedge-shaped, in radiating families . Gompkosphceria iie, W. Cells filamentous or ring-like, spirally twisted, usually motile, Spirillum, 1 07 W. Cells filiform, very minute, spirally twisted . , Spirulina, 10& X. Sheath present, gelatinous or jelly-like, often colorless (Y). X. Sheath none; cells spherical or angular by pressure, solitary or in small families . . ... ... Chroococcus, 121 Y. Aquatic (a). Y. On moist timbers and earth; cells small, in globose bodies in a thin membrane .... . ... Microcystis, 116 a. Sheaths thin, not confluent; cells tubular, short, parasitic, Chamasiphon, 100 a. Sheaths confluent, in a mucous stratum, or elongation or membra- nous (1^). a. Sheaths bladder-like, colorless; cells cylindric, ends rounded, Glxothece, 109 b. Sheaths confluent (<:). b. Sheaths membranous (/). c. Cells spherical or nearly so (d). t. Cells not spherical (e). GENERA OF ALG/E. 33 d. Tegument mucous, cells spherical or elongated, one pole color- less, not in families . . Hy drums, 52 d. Tegument mucous, with small indistinct families of innumerable cells . . . . . ... . . Anacystts, 117 d. Tegument soft, thick . . . . Aphanocapsa, 120 d. Tegument a mucous stratum, containing groups of two cells, these surrounded by another tegument . . . Glceocapsa, 119 d. Tegument a mucous stratum; thallus spherical, hollow, cells many, small, on the surface . ... Ccelosphmrium, 113 e. Tegument confluent, firm; cells longer than broad, Aphanothece, 110 c. Tegjument inconspicuous; cells elongate, cylindrical, Synechococcus, i ii e. Tegument soon perforate, breaking into lobed forms; cells very small, embedded Clathrocystis, 118 /. Cells globose, united in families, clustered grape-like, Polycystis, 118 g. Irregularly branched, often closely interwoven; stems and pri- mary branches of the same thickness; often aerial, Chrookpus, 22 g. Cruciformly branched, ends acute; filaments articulate or not, Asterothrix, io6a g. Somewhat dichotomously branched, apices rounded; floating and yellow, or not yellow and coating wet rocks . . Calothrix, 81 h. Filaments erect, laterally adherent, basally attached, forming layers on flat surfaces . ... . Isactis, 83 h. Filaments distinctly sheathed at base, sheaths broad, often sac- cate, transversely plicate; heterocysts basal . Glaotrichia, 84 h. Filaments agglutinated by mucilage, and forming hemispherical or bladdery forms . . .....*.. Rivularia, 85 PART II. Key to the Species of the Fresh-Water Algse. KEY TO THE SPECIES OF THE FRESH WATER ALG^. 1. LEMANEA. A. Filaments straight, 4-5' in. long, . . . fluviatilis, 52 A. " arcuately curved {a). a. 1-2 in. long, olive green, black with age. . . torulosa 5 1 a. 5 in. long, violet in mass; regularly constricted . . catenata, 52 2. TUOMEYA. A. Much and irregularly branched; tufts 1-2 in. long . fluviatilis, 53 A. Not branched, ..... . grande, 53 3. BANGIA. Filaments about i in. long; in lax purple tufts atro-purpurea, 55 4. BATRACHOSPERMUM. A. Branches sometimes setigerous, internodes naked or nearly so; gelatinous. Variable . . . ... monilifo7-me, 56 A. Branches not setigerous, whorls imperfectly developed, the branch- lets often impacted; color bluish green . . . vagum, 57 5. THOREA. Much branched, ramuli horizontal, long and short alternating, ramosissima, 5 8 6. CHANTRANSIA. A. Tufts I in. long; filaments with many straight branches; fertile branches very short . . macrospora, 59 A. Tufts 1-6 mm. long (a). a. Parasitic on other plants (b). a. Not parasitic (c). b. Fresh water; bright violet, 2 mm. long; branches erect, ends obtuse, violacea, 59 38 SPECIES OF ALG^. b. Marine; 1-4 mm. long in close fringe; branches rarely opposite, often hairy . . virgatula, 61 c. Terminal cells cuspidate or piliferous .... Hermannii, 60 c. " " rounded pygmcea, 61 7. HILDEBRANTIA. a. Fresh water; cells red; on river stones rivularis, 61 a. Marine; cells spherical ... . rosea, 62 8. COMPSOPOGON. One species .... cceruleus, 62 9. COLEOCHiETE. A. Filaments irregularly disposed, variable in form . irregularis, 65 A. " radiating (a). a. Filaments not laterally adhering, forming small disks . soluta, 64 a. " adhering side to side, forming a disk (U). b. Disk irregularly orbicular; cells subquadrangular . . scutata, 64 b. " exactly orbicular, thin, flat, . . orbicularis, 64 b. " orbicular, upper surface rounded, about 2 mm. high, pulvinata, 64 10. (Edogonium. It is only possible to identify the sixty-three species of this genus when the plants are in fruit, the forms being arranged in groups ac- cording to some characteristic or peculiarity of the oospore (the seed or spore), and of the oogonium (the sac containing the oospore). It is therefore scarcely possible to devise an artificial key. § Male and female organs on the same plant (A). § Male and female organs on different plants (B). § Male organs of fructification not known (C). A. Oospores globose or nearly so (D). A. " elliptical or ovate (f). B. Males short (dwarf), i-celled (k). B. Males short (dwarf), 2 — many-celled (/). B. Males filamentous, many-celled plants i^gg^. C. Oospores globose or nearly so (//). C. " elliptical or ovoid (ss). D. Oogonia globose or nearly so (a). D. " elliptical or ovoid (K). a. Oogonia opening by a pore (p). a. " " " " lid (/). b. Pore median; veg. cells 4-6 times as long as wide . cryptoporum, 70 SPECIES OF ALG^. 39 b. c. c. d. d. d. e. e. /■ f. g- g- h. i. i. J- J- k. k. I. I. m. tn. m. n. n. o. o. P- P- §■ r. r. s. s. t. t. u. u. Pore above the middle ic). Oospore not quite filling the oogonium (d). Oospore entirely filling the oogonium (e). Veg. cells 3-5 times as long as wide . . 2-5-3-5 4-10 a u u Veg. cells 4-5 times as long as wide 2-4 obsoletum, 71 pliisiospermum, 7 2 polymorphum, 73 fragile, 71 zig-zag, 71 Oospores subglobose, filling the oogonia . . . autumnale, 73 Oospores obovate, not entirely filling the oogonia (^). Veg. cells 4-6 times as long as wide . . . vernale, 72 Veg. cells 2-4 " " " " " _ crispum, 72 Oogonia single, opening by a superior pore; oospore globose not filling the oogonium ... . urbicum, 73 Oogonia opening by a pore above the middle (7). Oogonia " " " lid . gracillimum, 74 Veg. cells 3-7 times as long as wide . paludosuin, 74 " " 2-5 " " a II u _ crassum, 74 Oogonia with 7-12 vertical constrictions Oogonia without vertical constrictions (/). Oospore globose or nearly so (in). " subellipsoid [o). Diameter veg. cells 15-1 7/<, 4 times constricted " " " 28-38yu, not constricted " " " from 9-15// («). oospore depressed-globose " exactly globose . . Dwarf males adhering to all parts of the female " " on or near the oogonia Oospore membrane smooth (^). " " beset with bristles (cc). Oospores globose or nearly so (f). " ellipsoid or ovate (w). Oogonia opening by a lid.(j-). " a pore (i). Terminal cell rounded " " apiculate . . Pore in the middle or a little above (u). " above the middle (v). Veg. cells 4.5-6 times as long as broad (( it 2— A " " " '' " platygynum, 75 undulaium, 76 cataractum, 77 Hens, 75 Areschougii, 76 multispora, 78 cyathigerum, 77 Lundense, 79 macrandrium, 80 flavescens, 78 Braunii, 79 40 SPECIES OF ALGjE. V. Diameter of veg. cells 13-14/* ... ... pyriforme, 95 V. " " " " i6-2o/t . . ... irregulare, 79 V. " " " " 27-30/i . . \ . . . . crrssiusculum, 80 w. Oogonia opening by a pore {x). w. " " " " lid (aa). X. Pore above the middle (y). X. " below " " oospore with 4 spiral ridges . Huntii, 85 y. Oospore longitudinally costate . . .... Wolleanum, 82 z. Diameter of oogonia 45-50/* by 60-75/* • ■ • Boriseanum, 81 z. " " " 70-83/t by 90-105/* . . . concatenatum, 81 z. " " " 29-32/* by 33-38/* .... sexangulare, 82 aa. Oospore longitudinally costate, filling the oogonium, acrosporum, 83 aa. " not " " (dd). bb. Terminal cell setiform . ciliatum, 84 bb. " '' not setiform obtruncatum, 84 cc. Oospores ellipsoid oogonia opening by a pore in the middle, Hystrix, 87 cc. " globose (dd). dd. Oogonia opening by a pore at the middle . echinospermum, 86 dd. " " " " " above the middle {ee). ee. Spines of oospores conical (//). ff. Diameter of veg. cells 2.5-5 times as long as wide . stellatum, 85 ff. " " " " 1-2 times as long as wide . Donnellii, 85 ff. " " " " 6-14 " " " " " . echinatum, 86 gg. Oogonia not or scarcely swollen (hh). gg. " manifestly swollen («). hh. Diameter of oospore 22-52/* by 38-62/* .... capillar e, 87 hh. " " " 47-49/* by 50-66/* .... stagnale, 87 ii. Oospores globose or subglobose (/z). a. " ellipsoid or oval {pd). jj. Oogonia opening by a pore (kli). jj. '• " " " lid; oospore globose , . Fringsheimti, 90 - kk. Pore in the middle; membrane of veg. cells and of oogonia spirally punctate .... . . . . . . puncto-striatum, 90 kk. Pore in the middle; membrane not punctate . . rufescens, 89 kk. Pore above the middle (//). //. Oospore filling or barely filling the oogonia (mm). II. Oospores not filling the oogonia (nri). mm. Oospore globose, filling the oogonium, diameter 25-29/t by 24-30/* Franklinianum, 89 SPECIES OF ALG^. mm. Oospore sub-globose, barely filling the oogon by 46-56>' nn. Diameter of oospore 37-45/^ by 40-50/^ nn. " " " 5i-54>« by 73-85/* nn. Diameter of oospore 42-6oyU by 42-60/* . 00. Diameter of oogonia 40-45/i by 8o-ioo/* 00. " " " 63-75/* by 105-120/* 00. " " " 70-85/t by 130-160/* 00. " " '' 90-100/* by 130-150 //. Diameter of veg. cells less than i6/< (gf). pp, " " " " i6/i or more (rr). qq. Diameter of oogonia 17-18/* by 20/* qq. " " " 24-28/* by 28-35/* qq. " " " 33-35M by 33-43M ^^. " " " 24-25/* by 26-28/* rr. Diameter of oogonia 36-40/t by 44-45/* rr. " " " 61-75/t by 68-80/* . ss. Diameter of veg. cells less than 25/* (/■/). i.r. " " " " 25 /* or more [uu). tt. Diameter of oospore 35-36/* by 45-48/* tt. " " " 15-16/* by 17-19/* uu. Diameter of oospore 54-65/* by 75-103/* «a. " " " 45-50/* by 68-72/* 41 ia, diameter 42-50/* carbonicum, 90 . capilliforme, 88 pachyandrum, 89 cardiacum, 90 Boscii, 91 Landsboroughi, 9 1 I'ivulare, 92 z^ar. major, 92 delicatiilum, 93 moniliforme, 93 Londiense, 94 hexagonum, 94 . fonticola, 93 princeps, 93 pyriforme, 95 longatum, 95 gigateum, 94 grande, 95 II. BULBOCH^TE. In reference to the sixteen species forming this genus, the remarks made in connection with CEdogoniutn might be repeated. The spe- cies can be identified only when in fruit; to make an artificial key is therefore almost an impossibility. . Replying to a personal inquiry in regard to the division of the species into monoecious and dioecious groups, with the presence of dwarf males on or near the oogonia of all the so-called dioecious forms, Mr. Wolle writes: "These male forms are not developed when they appear; they are supposed to have been formed in dis- tinct cells known as androsporangia, and when they have developed and escaped, they float about and then attach themselves on, at or near the oogonium, but they are not necessarily a part of the plant on which they may be seated." 42 SPECIES OF ALGjE. § Oogonia globose or nearly so (A). § " ellipsoid or nearly so; oospores longitudinally crenulate- costate (F). A. Dwarf males i celled, on or near the oogonia, elachistandra, 97 A. " " 2 celled (B). B. Basal part (stem) of dwarf males, shorter than the spermogonia (C). B. " " " " " " longer than the spermogonia; oospore warty ... gigantea, 99 C. Diameter of veg. cells 20/i or less (D). C. " " " " more than 2o/<; oospore granulate, setigera, 98 D. Oogonia without terminal seta intermedia, 97 D. " with terminal seta, or beneath a male cell (E). E. Dissepiment supporting oogonia in the middle of cell or slightly below . . crenulata, 97 E. Dissepiment above the middle of the cell .... polyandra, 98 E. " below the middle ... . . Brebisonii, 99 F. Monoecious (G). F. Dioecious (H). G. Diameter of oogonia '2.'i-Z'ilJi by 46-56/* . . . mirabilis, 100 G. " " " 20-25/^ by 33-36/* .... nana, 100 H. Dissepiment of cells supporting oogonia obsolete (I). H. " " " " " present (J.) I. Diameter veg. cells 12-5/t, length less than diameter, /j/^/««a, loi J. Diameter veg. cells i6/t or less (K). J. " " " more than 16/i (L). K. Veg. cells 1-1.5 times as long as wide . . . subsimplex, loi K. " " 2-3.5 " " " " " .... repanda, 102 K. " " 1.5-2 " " " " " . rhadinospora, 103 L. Veg. cells 2.5-4.5 times as long as wide . . . insignis, loi L. " " 1.5-3 " " " " '1 minor, loi L. " " 1.25-3 " " " " " . . rectangularis, 102 12. SPH^ROPLEA. Cells 8-10-20 times as long as broad, chlorbphyllose rings 20-30 In each annulina, 104 13. CYLINDROCAPSA. Ends of cells clear and pearly amxna, 105 Ends of cells not clear . . . . . . geminella, 104 SPECIES OF ALG^. 43 14. PRASIOLA. Tufts 2-6 mm. high and broad, cells 4-6jx in diameter, crispa, io6 " 1-3 inches long and broad, cells 6-8/t " " Mexicana, 107 15. ENTEROMORPHA. § In brackish water {a). a. Fronds simple, elongate; cells 3-5-6 angled . intestinalis, 107 a. " compressed, tubular .... ... compressa, 107 16. DRAPARNALDIA. A. Every mature joint with 2-4 clusters of branches, Ravenellii, no A. Branches less abundant, not at every jomt (a), a. Branches opposite, their stems thick, short, tapering to a point or seta ... . . . .... . spinosa, 109 a. Branches opposite or alternate . glomerata, io?>, plumosa, 1-09 17. STIGEOCLOklUM. § In warm or hot water; 1-2 in. long, much branched, apex a bristle, thermale, in § Not in water of high temperature [a), a. Branches ending in a hair or bristle (b). a. Branches not ending in a hair or bristle [k). b. Bristle a colorless, apparently hollow prolongation (c). b. Bristle a delicate, filamentous, hair-like extension (d'). c. Cells nearly cylindrical, equal or twice as long as broad, protensum, 112 d. Pa.rasitic, dwarf, 2 mm. long; in mucus .... radians, 115 d. Not parasitic (e). e. Diameter of largest cells 20/i or less (/). e. " " " " 25-40/^ . . . nudiusculum, 113 f. Diameter of filaments ii-20yu; basal cells 6-8 times longer than wide . ... subsecundum, 112 /. Diameter of filaments io-i6/i {g). g. Branches opposite on cells smaller and more oval than the others, flagelliferum, 112 g. Branch-bearing cells not smaller (Ji). h. Upper branches clustered, moniliform . fasttgiatum, 114 h. " " not moniliform (j). j. Two or more inches long, branches mostly opposite, fasciculare, 114 / Two to 10 mm. long, simple to near the end, there branches clustered longipilus, 115 44 SPECIES OF ALGJE. k. Length 4-50 mm.; somewhat branched, branches simple, ienue, no k. " 2-3 mm.; branches alternate, ends obtuse, . nanum, 112 k. " 12 mm.; branches mostly opposite, ends acute, amasnum, 113 18. CHvETOPHORA. § Jelly mass globose or nearly so (a). § Jelly mass elongated to 3 inches, margins tattered, forming ragged , branches . . .... . . . endivicefolia, 117 § Jelly mass orbicular, small; filaments hair-pointed . longipila, 118 a. Branches more or less moniliform; jelly mass 2 mm. diameter, monilifera, 118 a. Branches not moniliform, radiating (b). b. Jelly mass smooth (c). b. " " tuberculose, ends of branches rarely hair-like, tuberculosa, 116 c. Diameter of branchlets 6/^; 1.5-3 times longer . pisiformis, 116 c. " " branches 7-10//; 1-1.5 " "... elegans, 116 19. MICROTHASJNION. But one species . . . .?■ . . . Kuetzingianum, 118 20. APHANOqjjI^TE. § Parasitic (a). § Not parasitic {b). a. Cells globose, a bristle on the back . . . repens, 119 a. Cells various, elongate, curved, worm-like, sub-globose, vermiculoides, 119 b. Cells globose, or subpyritorm, a seta on the back or apex; in mucus . . . globosa, 119 21. GONGROSIRA. One species . . . Sclerococcics, 120 20. CHROOLEPUS. a. Cells globose, broadly elliptic or ovate (V). a. Cells more or less elongate (c). b. Dull or bright red; diameter 14-2 2/^ . . . umbrinum, 123 b. Ash color when dry; diameter 20-25 .... montliforme, 123 c. Diameter of cells more than 25-40/.*; branches rather dichotomous, lolithus, 122 c. " " " less than 2S/i ((/). SPECIES OF ALGiE. 4S d. Stratum tawny red, drying greenish; diameter of filaments 9-14//, odoratus, 122 d. Stratum orange-red, drying yellow; diameter of filaments 7-9/f, lichenicolus, 122 d. Stratum red or orange, soft, silky; much branched; diameter of cells 10-12/^ ... ... aureus, 121 23. CLADOPHORA. § Chlorophyll diffused, not in spirals (a). § " disposed to be net-like or in lax spirals (d). a. Cell membrane smooth (h). a. " " plicate-striate; contents of young slightly spiral, crispata, 126 b. Cells more or less swollen; irregularly branched; variable, fracta, 1 24 b. Cells not noticeably swollen (c). c. Diameter of branches 2 8-40 yu . , . . oligodona, 126 c. " " " about is/< . . . Flotowiana, 126 d. Branches united (connate) at base; cells 5-8 times as long as broad, canilicularis, 117 d. Branches not connate at base (e). e. Filaments not radiating from common centre, cells smooth (/). e. " " " cell membrane plicate . flavescens, 128 e. " radiating from a common centre, much branched, CBgagropila, 124 /. Diameter of stems 60-100/^ ... . glomerata, 127 /. " " " 120-135/* .... . . fluitans, 128 24. PITHOPHORA. A. Fertile stem d^jx thick .... . CEdogonia, 130 A. " " 175/^ thick . . . . CBqualis, 131 A. " " 59/4 thick . . . Kewensis, 131 24a. SCHIZOMERIS. One species . . . . ... Leibleinii, PL CXXV. 25. ULOTHRIX. § Length of cells equal to width {a). § " " " greater than width [e). § " " " less than width {h). a. Color dark, light or bright green {b). a. Color yellowish green [d). 46 SPECIES OF ALGiE. b. Filaments dividing longitudinally; mucous; 4 in. long, floating, Lenormandi, 138 b. " not dividing longitudinally (c). c. Joints often constricted; diameter of cells 9-iOyU . rivularis, 136 c. Joints not constricted; aquatic; variable; diameter of cells 5-50/*, sub tilts, 135 c. " " " " filaments tortuous, very long, muralis, 137 {. " " " On damp walls, pavements, exposed rocks, etc., (*) *. Cells 6-7/^ in diameter; on walls, pavements, etc. . mitens, 137 *. " 7-9.5/t " " cell contents usually at one side; on ex- posed rocks, etc. . . . flaccida, 137 d. Cells i2-i4/< in diameter . aqualis, 134 d. Cells about ijx in diameter; on moist clay or sand . . varia, 138 e. Green, cell contents usually on one side, hemispherical, flaccida, 137 e. Green, dark, bright or pale; contents diffused (/). /. Joints often constricted; cells 9-io/t in diameter . rivularis, 136 /. " not constricted; filaments attached, 17-25/^ in diameter, tenuis, 134 /. " " " filaments not attached {g). g. In brackish water, filaments 1-3 in. long, io-30/t dia., Younganum, 135 g. In fresh water subtilis, 135 h. Filaments dividing longitudinally, 4 in. long, floating, Lenormandi, 138 h. " not dividing (i). i. Joints often constricted, cells 9-iOyM in dia.. . rivularis, 136 /. " not constricted in sterile filaments (7). j. Filaments lofjL or more in diameter (K). j. " less than loyu in diameter (m). k. In brackish water; filaments 20-30/* in dia. . Younganum, 135 k. In brackish and in fresh water; filaments 38-5o/< in dia., speciosa, 135 k. In fresh water only or on the ground (/). /. On shaded ground; flexuous, interwoven. . parietina,- 138 /. Aquatic; filaments io-i2yU in dia. . . . oscillarina, i^y I. " " 14-40/i" " Zonata, 133 m. Bright, grass green; filaments tortuous, very long, interwoven, muralis, 137 m. Yellowish green, mucous; cells shorter than wide . compacta, 1^6 SPECIES OF ALG^. 47 26. SCHIZOGONIUM. One species; found on moist cliffs, Colorado . . murale, 139 27 CONFERVA. § In brackish water; diameter i2-i4yU fugacissima, var. saliria, 141 § In fresh water (A). A. Diameter of filament from 3-5 yU {a). A. " " " from 6-12/^ (^). A. " " " from i4-25ya [g). a. Very pale; cells 1^-2-3 times as long as wide . tenerrima, 143 a. " " " 4-9 " " " " " . glacialioides, 143 a. Green; cells 3-4 " " " " " . . rhypophila, 143 b. Cells before division 7 times as long as wide . . . affinis 141 b. " " " 4-5 " " " " " (c). b. " " " less than 4 times as long as wide (if). c. Not constricted atthe joints ... ... fugacissima, 141 c. Slightly " " " " (d). d. Cells before division about 4 times as long as wide . Funkii, 142 d. " " " " 5 " " " " " filaments of ir- regular thickness . . .... bumbycina, 142 e. Cells more or less swollen {*) e. Cells not swollen, joints not constricted (/). * Diameter of filaments 10-12// . . ... vulgaris, 142 * " " " 7-9y" .... . var. Farlowii, 142 /. Attached; chlorophyll evenly diffused . . abbreviata, 143 /. Not attached; contents granular . . . • . . punctalis, 142 g. Diameter of filaments 20-25// ... . amcena, 140 g. " " " less than 20/t (/;). h. Cells more or less swollen, about twice as long as wiAe.,floccosa, 140 h. " " " " constricted at the joints, utriculosa 140; fontinalis, 141 28 CH^TOMORPHA. Marine, therefore omitted. 29 RHIZOCLONIUM. § On moist ground; cells 1.5-3 times as long as wide, hieroglyphicum, 1 44 § In brackish water (a). § In sweet waters {I)). a. Filaments irregularly curved and angled. California, Casparyi, 145 a. Filaments not specially curved; diameter 20-30/t . . salinum, 145 "^ 33-40yM . . major, 146 48 SPECIES OF ALG^. b. Permanently attached, in rapid streams . . . fluitans, 145 b. Not permanently attached {c). £. Diameter of filaments 13-15. 5;u . . . fontitMli, 144 c. " " " 2S-33yU stagnale, 145 c. " " " 36-40yU . . Horsfordii, 145 30. VAUCHERIA, I. KEY TO THE STERILE PLANTS. § In salt or brackish water; mats velvety, feet in extent, Thurdii, 149 § In sweet water or somewhat terrestrial {a). § Apparently terrestrial only (h). a. Filaments dichotomous, branches constricted at the base of the divisions, often intermediately, .... . tuberosa, 154 a. Filaments not constricted at the branching points {b). b. Stratum dense, pellucid below, creeping; terminal branches erect, clustered, often crooked . ... velutina, 153 b. Stratum not pellucid below {c). c. Branches not clustered, vague. Thus far found only in California, hamata, 153 c. Branches dichotomous or sparingly formed {d). d. Diameter of filaments loofx or more (e). d. " " " less than ioo/.t [g). e. Diameter 100 to 2oo/< (/). /. Diameter 125;.*; tufts dense, dark green . . geminata, 151 /. " loo/t; tufts loose, expanding in all directions, aversa, 149 /. " ioo-2oo/t; dirty green or brownish; about limestone springs ... . . dichotoma, 149 /. Diameter 1 8o-2ooyU, often hyaline; drying ash gray . pilus, 153 g. In turfy mats; filaments densely intricate; 50-75// in diameter, se7-icea, 150 g. In dense, intricate tufts; filaments dichotomous, 50// in diameter, geminata, 151 g. In loosely intricate clusters; filaments about 50/1 in diameter, sessilis, 151 h. Stratum thin, expanded, filaments about 50/* in diameter, Dillwynii, 150 h. " " densely interwoven, dark green . . terrestris, 153 SPECIES OF ALG^. II. KEY TO THE FRUITING PLANTS OF VAUCHERIA. 49 § Antheridia slightly bent; the opening at top (a). § " bent like a horn or hook (^). § " not known (g). a. Oogonia nearly round (6). a. " not round; oval, often rather oblique (c). b. Oogonia about ioo/< in diameter .... dichotoma, 149 b. " i25-i20;u " " Thuretii, 149 c. Oogonia i-6, on one side, mouths lateral, produced beak-like, sericea, 150 c. " erect, mouths not lateral, not produced beak-like, aversa, 149 d. Antheridia beside or between the subsessile oogonia (1?). d. Antheridia terminal, with the oogonia on each side or lower down (/). e. Terrestrial, broadly expanded; antheridia bag-shape or clavate, Dillwynii, 150 e. Aquatic; antheridia short and straight, or elongate and variously curved . . . . . sessilis, 151 /. Tetrestrial only; antheridium long, curved, oogonia on its back, terrestris, 153 /. Not strictly terrestrial; fruit on short lateral branchlets, geminata, 151 /. " " " fruit on short segments on the divided ends of branchlets ... . hamata, 152 g. Branches regularly constricted at base and often elsewhere, tuber osa, 154 g. Branches not constricted; filaments i8o-2oo/< in diameter, pilus, 153 One species One species One species One species 31. BOTRYDIUM. 32. VOLVOX. 33. EUDORINA. 34. PANDORINA. granulatum, 155 globator, 158 stagnale, 160 morum, 161 50 SPECIES OF AWJiE. 35. EUGLENA. The question as to the animal or vegetal character of Euglena has been debated. The present tendency is to consider it an alga, although the evidence is rather negative than positive. For the present purpose however, it seems best to leave it among the Infu- soria, where the older investigators classed it. 36. GONIUM. One species . . . . pectorale, 163 37. STEPHANOSPHjERA. No forms yet found in this country. 38. CHLAMYDOCOCCUS. a. Cells subglobose, brownish-red, sometimes green; variable, pluvialis, 164 a. Cells globose, red; at first with a hyaline border; on the snow of high peaks, etc. .... . . . nivalis, 166 39. CHLAMYDOMONAS. § With a red pigment spot (a). , § Without " " " (b). a. Ovate; length twice the width; pigment spot lateral, pluviusculus, 168 b. Pale, whitish green; oblong-elliptical . hyalina, 168 b. Bright light-green {c). c. Ovate or oval . . . iingens, 167 c. Spherical or nearly so . .... pluviale, 167 40. pediastrum. For full descriptions and figures of the species, see Wolle's "Des- mids of the United States," from which the following classification is compiled. § Apices cuspidate, each cell with one cusp or bristle {a). § " " " " " two " " " (c). § Apices or angles, bidentate; small; cells often 4 . cuspidatum, 154 § Apices truncate; cells 4 in form of cross, 4-sided; small, tetras, 154 § Apices concave, undulate (d). § Apices bilobed (if). a. Cells six, ovate or subfusiform, radiately connected; centre closed, simplex, 152 a. Cells six, 5 angled; centre open . . . Sturmii, 153 a. Cells more than six (b). SPECIES OF ALG^. 51 b. Outer circle 14, inner 7, centre closed by i; outer spaces 7, inner 4, simplex var., 153 b. Outer circle 12, inner 4, centre open; outer spaces 4, duodenarius, 153 b. Outer circle 14, inner 7, centre closed by 4; outer spaces 7, inner 4, simplex var., 153 c. Marginal cells 2-lobed, each cuspidate; cells 8-16-128; centre closed, Boryanum, 153 d. Perforated by small openings ... . brachylobum, 154 d. No openings; centre closed; . . muticum, angulosum, 153 e. Perforate by small openings, or centre open (/). e. Not perforate, centre closed {g). f. Cells 16-32-64 . . . /. Cells 6, centre open . g. Apices converging, space between oval g. Apices not converging (li). h. Each apex 2-lobed, sometimes diverging pertusum, 154 angulosum, var., 154 forcipatum, 153 Ehr enter gii, 154 utriculatum, 169 microporum, 170 cambricum, 170 spinulosum, 171 cruaatum. 41. HYDRODICTYON. One species 42. CCELASTRUM. a. Cells globose, with interstitial openings . . a. Cells angular, each with a tubercular process 43. SORASTRUM. One species 44. STAUROGENIA. One species . . 45. SCENEDESMUS. § Cells armed with spines (a). § Cells not armed with spines {e). a. Aquatic (b). a. On moist sand, Florida; cells 2-4, fusiform, antennatus, var. recites. b. Cells oblong, cylindrical, ovate or globose (c). b. Cells, some at least, spindle-shaped [d). c. Spines recurved on outer cells c. Spines straight, single or sometimes two c. Spines straight, three to five d. Inner cells fusiform, armed, outer lunate, d. Fusiform, oval or globose; spines straight 171 172 caudatus, 172 polymorpkus, 173 . rotundatus, 174 dinwrphus, 173 polymorpkus, 173 52 SPECIES OF ALGjE. e. Cells, some at least, fusiform (/), e. Cells oblong or ovate, obtuse; 3-5 times as long as wide, obtusus, 173 /. Cells in a single even row; outer cells lunate . . dimorphus, 173' /. Cells usually all alike, acute, 3-6 times as long as wide, acutus, 173 46. SCIADIUM. a. Attached, umbellate; cells usually straight; base contracted into a short stem arbuscula, 1 74 a. Floating; a collection of umbels connected by a usually straight cell ... gracilipes, 175 47. OPHIOCYTIUM. § One or both ends spinous (a). § Without spines (c). a. Both ends spinous {J)). a. One end spinous, one end rounded; cells curved or coiled, cochleare, 175 b. Cells arched or nearly straight, ends more or less enlarged, capitatum, 176 b. Cells crescentic, Closterium-\ik.t, large . cuspidatum, 176 c. Cells small, curved, often in masses, ends rounded, parvulum, 176 c. Cells long, narrow, repeatedly coiled, ends rounded, circinatum, 176 48. CHARACIUM. § Sessile, no stipe; cells pyriform, apex somewhat produced, sessile, 177 § Not sessile, stipe present but often short (a), a. Apex rounded or truncate (b). a. Apex acute (d). b. Stipe short, scarcely noticeable, end dilated; cells producing a short neck acutum, 177 b. Stipe longer, noticeable (c). c. Apex produced as a thick neck; stipe brownish yellow, Pringsheimii, 177 c. Apex always rounded, cells obovate, stipe base not dilated, Ncegelii, 178 c. Apex rounded, truncate when open; stipe hyaline, heteromorphum, 178 d. Producing a short neck; cells ovate acutum, 177 d. No distinct neck; lanceolate or sword-shaped, ends tapering, ambiguuin, 177 SPECIES OF ALG^. 53 49. PROTOCOCCUS. § Aerial forms (a). § Aquatic (c). a. Cells without gelatinous envelope (i). a. Cells with gelatinous envelope in which they divide; angular from pressure angulosa, i8i b. Common everywhere on trees, fences, etc.; cells yellowish green, viridis, 181 b. On moist old wood; cells dark green; decussately dividing, dissectus, 18 r b. On pots, etc., in green-houses; orange, reddish-brown, yellowish green miniatus, 182 c. Beset with small spines or hairs vestitus, 183 c. Smooth, in gelatinous tegument, . . . humicola, -l?i2; gigas, 183 c. " not in tegument [d). d. Cells clustered somewhat grape-like .... botryoides, 182 d. Cells single, membrane thick, often lamellate . Wimmeri, 183 d. Cells varying in size, membrane not lamellate . . infusionum, 182 50. POLYEDRIUM. § Angles rounded, not spinous (a) § " bifid or trifid {b). § " spinous or greatly elongated {c). a. Cells 4 or 5 angled, diameter 35-70/1 . . . gigas, 184 a. " 4 angled, diameter 8-io/< minimum, 185 a. " 3 angled, diameter i2-i5yu . .... muticum, 185 b. Angles bifurcate, sharply tipped .... . bifurcatum, 184 b. " often repeatedly bilobed, hyaline, acute . . enorme, 184 c. Centre scarcely body-like, radii thin, much prolonged, longispinum, 185 c. Centre distinctly a body; angles spinous tetragotium, minus, 184 51. DICTYOSPHjERIUM. a. Cells ovate or subglobose . . Ehrenbergianum, 186 a. Cells reniform, about twice as long as wide . reniforme, 186 a. Cells oval, often centrally constricted . . . Hitchcockii, 186 52. HYDRURUS. One very variable species .... . fmtidus, 187 53. PALMODACTYLON. a. Family of cylindrical, bladder-like radiating cases; cells in i or more rows ... . variwn, 189 a. Family simple, not branched, more filamentous . . simplex, 189 54 SPECIES OF ALGiE. :>/ / -r ' •'■'' 54. TETRASPORA. /3^/^/' /'//• ,S ///(•• § Thallus tubular, splitting (a). § Thallus irregularly expanded (U). § Thallus membranous, saccate, obovate, sinuate, buUose (c). a. Cells about i6/i in diam.; ends tapering to a fine point, cylindrica, 1 90 a. Cells 8-io/f in diam. ; yellowish-green lubrica, 191 i. Colorless, or green edged gelati?wsa, 191 J>. Green, rather soft and slippery explanata, 191 £. Dark green, smooth or somewhat warty; cells crowed in 2s or 4s, bullosa, 190 55. SCHIZOCHLAMYS. Supposed to be a form of Tetraspora. 56. PALMELLA. § Aquatic {a). § On wet ground or dripping rocks (V). § On wet wood . uvcsformis, 193, botryoides, 194 a. Irregularly expanded; cells minute (o. 75-1. 00/t), crowded, hyalina, 193 a. Somewhat globose, tuberculate; cells elliptical, 5 by 8/i, Mooreana, 193 b. Thallus expanded or indefinite . . . mucosa, 192, miniata, 193 57. PORPHRYDIUM. One species; terrestrial cruentutn, 194 58. BOTRYDINA. One species; terrestrial . vulgaris, 195 59. BOTRYCOCCTJS One species; aquatic Braunii, 195 60. GLOeOCYSTIS. § Aquatic (a). § On wet rocks and walls; cells 3-S;U in diameter . . rnpestris, 196 a. Dirty or bright green; families 45-90// in diameter . ampla, 196 a. " " " " families about 35// in diameter, vesiculosa, 196 a. Brownish-yellow or reddish rufescens, 196 61. NEPHROCYTIUM. Families oblong or elliptical, cells reniform . . Agardhianum, ig"] Families subspherical, cells oblong or elliptical . . Ncegelii, 197 SPECIES OF AhGM. 55 62. RHAPHIDIUM. a. Cells straight or nearly so, length 15-20 times the diameter, acinilare, k)"] a. " " " " " " 20-30 or more times the diameter, contorttim, 198 a. Cells decidedly curved; often crescentic or sigmoid {I)). b. Cells sigmoid, single or 2-4 connected .... sigmoideum, 198 b. Cells more or less crescentic (<:). c. Cells connected back to back, crescentic, sometimes single, convolutum, 198 c. Cells not connected, long, slender, .... falcatum, 198 c. " " " short, thick, diameter 5-7/* . Braunii, 198 63. DltlORPHOCOCCUS. One species . . . . cordatus, 199 64. MISCHOCOCCUS. One species . . . . . . ., . confervicola, 200 65. EREMOSPHjERA. One species . viridis, 200 66. UROCOCCUS. a. Stem densely ringed, often divided .... Hookerianus, 201 a. Stem short, remotely annulated . insignis, 201 67. APIOCYSTIS. One species . . ... Brauniana, 202 68. CHYTRIDIUM. a. On the oogonia of various ffii^^^^^/a; cells somewhat urn-shaped, with a lid acuminatum, 202 a. On various filamentous Algae (i^). b. With a low lid; pale yellow . . . . minus, 203 b. Without a lid; cells spherical ...... globosum, 203 69. OLPIDIUM. § On the exterior of the host; cells globose; mouth cylindrical, nearly as long as the cell ampullaceum, 203 § In the interior of the host; perforating neck tubular, middle globosely dilated . . intestinum, 203 § In the interior; perforating neck straight, not inflated, entophytum, 204 56 SPECIES OF ALG/E. 70. SPIROGYRA. I. KEY TO THE STERILE PLANTS. As it is often difficult to determine the number of spiral bands within a cell of Spirogyra, the following method, originally suggested by "The Botanical Gazette," will be found exceedingly convenient. Select a band near the middle of a cell, count those that cross it, add one to the number, and the result will be the whole number of bands in the cell. § Membrane of ce'.ls folded in at the ends (a). § " " " not folded in at the ends (y). a. Chlorophyll bands one only (U). a. " " two or more {g). b. Membrane of spore smooth (^ to 6 turns, Weberi, 208 e. Cells 4-15 times as long as wide; spiral of 3-6 turns, tenuissima, 207 e. " 3-8 " " " " " spiral of 3-8 turns, inflata, 207 /. Diameter of filaments 36-40//; cells 6-12 times the diameter, calospora, 209 g. Spirals two (K). g. Spirals three; cells 30-33// wide, 4-8 times as long as wide, Hassallii, 210 h. Cells i6-22// in dia., 4-8 times as long as wide, . Hilseana, 209 h. Cells 3o-33/< " " 4-8 " " " " " . Hassallii, 210 h. Cells 36-45// in diameter (/). /. Cells 4-12 times as long as wide . insignis, 210 i " 3-5 " " " « '< Hantzschii, 211 j. One spiral in each cell (k). j. Two or more spirals in each cell (t). SPECIES OF ALGjE. 57 subsalsa, 212 varians, 212 quinina, 213. condensata, 215 k. Membrane of spore smooth (/). k. " " " punctate {s). I. Cells less than twice as long as wide [m). I. Cells twice or more " " " " («). ■m. Diameter 22-25/t; i-ij4 times as long as wide, m. " 25-33/*; i-2y2 " " " " " m. " 33-40//; 1-2 rarely 6 " " " " m. " 48-75/4; i-ij^ times " " " n. Diameter 33/* or more (o). n. " less than 33/* (^). (7. Diameter 33-40/i; 1-2 rarely 6 times as long as wide, quinina, '213 o. " 30-5S/" (/)• /. Cells 3-7 times longer than wide; spiral dentate . Lutetiana, 214 p. " 2-4 " " " " fusco-atra, 215 q. Cells 3-5 times as long as wide (f). q. Cells 2-12 times as long as wide {gg). r. Diameter 22 /i or less . flavescens, gracilis, 211; communis, 213 r. " 24/i or more . . varians, 212; Jurgensii, 213 ^. Diameter of cells 24-27/i, 6-12 times longer than wide, /a;zrf«^a, 215 t. Spirals 2 («). /. " 3 (x). /. " 4 {aa). t. " 5 (^^). t. " 6 or more (ff). u. Diameter of cells 40/* or less (v). u. " " " more than 40/t (w). w. Cells 2-4 times as long as wide decimina, 216 V. " 4-14 " " " " " . elongata, 221 w. Cells 1-2 times as long as wide subcequa, 217 a/. " 1.5-2.5 " " " " " . . . dubia, 220 X. Cells 90-100/1 in diameter, joints somewhat constricted, jugalis, 219 X. Cells 50/1 or less in diameter (jc). X Cells more than 50/* in diameter (z). y. Cells 7-1 1 times as long as wide y. " less than 7 times as long as wide z. Cells 1-2 times as long as wide _g u 2 — -3 " '' " " " «. " 2-5 " " " " " z. " 2.5-10 " " " " " . rivularis, 220^ dubia, adnata, 217 . . subcequa, 217 orthospira, 21S neglecta, 226 :/(«, 221 S 58 SPECIES OF ALG^E. aa. Cells more than loo/^ in diameter (bV). aa. " from 72 to loo^u " " (cc). aa. " less than 70/t " " (id), bb. Cells 150-156/i in diameter (the largest species) . crassa, 219 bb. " 102-iioyW " " setiformis, 219 cc. Cells 90-1 00/t in diameter . . Jugalis, 219 cc. " ■j2-'j8jA " " 1-3 times as long as wide nitida; 217 cc. " 75// " " 2-4 " " " ' dd. Cells 58-65/^ " " ee. Cells 65-78/^ in diameter ^c. • " 58-65/* " " 7y. Filaments of two diameters, one 125//, the other 80/^, incequalis, 218 jy. " " same diameter, i5o-i56/( //". " " " " 102-iiOyU ^. " " " " 1 18-125// //. " " " " 54-62/^ gg. Cells 4-10 times as long as broad, 24-27/* diam. gg. " 2-12 " " " " " 25-30/4 diam. parvispora, 221 orthospira^ 218 fluviatilis, 216 be His, 217 ortkospira, 218 . crassa, 219 setiformis, 219 maxima, 218 tnajuscula, 221 mirabile, 211 longata, 214 II. KEY TO FERTILE FILAMENTS OF SPIROGYRA. For method of determining the number of bands in the cell, see the Key to the sterile filaments of Spirogyra, p. 56 § Cell membrane folded in at the ends ((z). § " " not folded in at the ends (7). a. Chlorophyll band single, rarely double (^). a. " " two or more {K). b. Membrane of spore smooth (c). b. " " " punctate {g). c. Spores elliptical or fusiform (d'). c. " ovate or oval (e). d. Spore 24-30/t by 50-58// . . . tenuissima, 207 '^- " 3°-36/< in dia., twice as long . . inflata, 207 d. " 27-32/t in dia., 2-4 times as long . . quadrata, 2c8 >. Mature spores yellow, oval, ^ longer than wide . neglecta, 216 y. " " not yellow {z). z. Diameter of filaments 90-100/4; spore oval . . . jugalis, 219 z. " " " 40-65/4 (a(j). 32-36/^ (dd). aa. Spore orbicular, flattened . . orthospira 218 aa. Spore not flattened (bb). bb. Spore spherical or broadly ovate . . . subczqua, 217 bb. Spore not elliptical or ovate (cc). cc. Spore oval-elliptical or subcylindrical . . dubia, adnata, 220- cc. Spore oval or subglobose, spore cells 2-4 times longer than wide, majuscula, 221 dd. Sterile cells 7-1 1, fertile cells about 4, times as long as wide, rivularis, 220 ee. Spore-bearing cells much inflated, spore oval, 80/t long, fluviatilis, 216 ee. " " " slightly or not inflated (77)- •, ff. Spore flattend, orbicular orthospira, 218 ff. Spore not flattened i^gg). gg. Diameter of filaments 150-156/1 . ... crassa, 219 gg. " " " io2-iio/t setiformis, 219 gg. " " " 90-100/1 ..'.... jugalis, 219 gg. " " " 72-78/1 (M). M. Chlorophyll bands making 1-4 turns in each cell . nitida, 217 hh. " " " I ^ " spore 50/1 wide, parvispora, 221 ii. Spores flattened, orbicular, membrane smooth . . orthospira, 218 ii. Spores " " " punctate or porose, bellis, 217 jj. Filaments of two widths, one 125//, the other 80/t, inmqualis, 218 jj. " of equal width (kK). kk. Diameter 150-156/1 crassa, 219 kk. " 118-125/1 maxima, 218 kk. " I02-II0/1 setiformis, 219 kk. " 54-62/1 majuscula, 221 SPECIES OF ALG^. 71. SIROGONIUM. One species 61 sticticum, 222 72. ZYGNEMA. §, Color soon becoming dark purple; filaments 20-25// in diam., purpureufti, 224 § Color green, or when in fruit yellowish or brownish (a), a. Filaments with gelatinous sheath; cells 25/t wide, anomalum, 224 a. " " " " cells 40-44// wide, crassum, 224 a. " without sheath (6). b. Spore membrane smooth {c). b. " " punctate or granulate {d). £. Diameter of filaments 20-22/* c. '• " " zt-2,ofJi . d. Diameter of filaments 10-36/*, very variable d. " " " 35-SO-W, ermum, 222 insigne, 223 siellium, 223 ci-uciatum, 2 24 73. ZYGOGONIUM. § Diameter of filaments 20/t or less (a). § " " " more than 20/* (c). a. Diameter of filaments 15-18/* .... ... Agardhii, 226 a. " " " i8-2o/t {b). b. Spore spherical . . .... ... decussatum, 262 b. " compressed ellipsoid, twice as long as wide . Ralfsii 221 c. Cells slightly constricted at the joints; spore punctate, pectinatum, 225 £. " not constricted {d). d. Filaments 30-33/t in diameter . aquale, 226 d. " 22-24/* " " • parvulum, 227 74. MOUGEOTIA. § Filaments 20-25/* ■'^ diameter (a). § " less than 20/* in diameter (^). a. Spore spherical, diameter about 40/* . . a. " " " " 45/* . b. Filaments 15-18/* in diameter b. " 10-14/* " " W- c. Spore spherical, 25-30/* in diameter *:. " oval, radiately corrugated, yellowish-brown ^/j^foj^frwa, 229 *:. " " coarsely granular, dark brown verrucosus, 229 spharocarpa, 227 divarecata, 228 Minnesotensis, 228 delicatida, 228 62 SPECIES OF ALGjE. 75. MESOCARPUS. § Diameter of filament S°M': spore smooth crassa, 230 § " " " i8>t or less((2:). § " " " 2o/< to 32/< {<■). a. Spore membrane punctate; filaments 9-15/^ diam. nummuloides, 231 a. " " smooth (^). b. Filaments 8-io/^ in diameter . ... . parvulus, 230 b. " io-i8yU " " recurvus, 231 c. Spore membrane punctate; filaments 25-32;^ in diam. robustus, 231 e. " " smooth {d). d. With short branchlets from the middle of a cell . radicans, 231 d. Without branchlets {e). e. Diameter of spores and filaments about equal e. " " " twice that of the filaments scalaris, 230 macrospora, 230 76. PLEUROCARPUS. Diameter of filaments 25-30^1* . ... 37-40/^ One species One species 77. PLAGIOSPERMUM. 78. GONATONEMA. . mirabilis, 232 Columbtanus, 232 tenue, 233 ventricosum, 233 79. STAUROSPERMUM. Diameter of filaments 14-20//, becoming bluish, spore smooth, capucinum, 234 " " " 8-1 2/<; spore punctate . . quadratum, 234 " " " 6-8/<; spore smooth . . . viride, 234 80. CRATEROSPERMUM. One species lativerens, 235 gracilis, 237 81. CALOTHRIX. § Diameter including sheath 6-8/i at base § " " " io-i4/< {a). § " " " iS-3°y"('^)- a. Sheath lamellose, ends colorless, torn into fibres; cells 6-8yu in diam. Meneghiniana, 238 a. " not fibrous at ends; brown or black in mass, gypsophila, 237 a. " " " " " bluish, rarely brownish, Dillwynii, 237 SPECIES OF ALG^. 63 b. Floating, dichotomously branched, apices slightly bent; diameter 15-20/^ . . ... lacucola, 239 b. Attached (c). c. Ends rounded, obtuse, fibrous with age, bluish crustaceum, 239 c. Ends tapering, or hair-pointed {d'). , d. Forming a stratum (/). d. Forming tufts {g). f. Branches tapering to a fine hair-point Horsfordii, 239 /. " cuspidate at apex or obtuse . . Orsiniana, 236 g. Tufts small, wavering; filaments somewhat curved, clustered, ra- diating; sheath up to 30yu, wide radiosa, 239 g. Tufts bright bluish, sometimes brown ; sheath widens with age, Brebissonii, 23 S 82. MASTIGONEMA. § Filaments rounded or truncate at apex (a). § " tapering to a hair-point (b). a. Sheath thick, apex truncate, open; diam. Zjx . fertile, 244. a. " in young plants ending in a seta, becoming open and trun- cate; diam 12 jj. . . . . ... Halos, 243 b. Mature sheath becoming fibrillose at the ends {c). b. Mature sheath not fibrillose or rarely so (d). c. Filaments 4|< in diam.; yellowish or greenish; parasitic, sejunctttm, 243 c. Filaments lo-ii/^ in diam.; bluish green; on wet xock=,, fibrosa, 244 d. Thallus subglobose or fusiform, blackish, firm; filaments bluish, diam. 6-7/* ... ... elongatum, 243 d. Not subglobose; filaments straight, bent or curved, cerngineum, 241 83. ISACTIS. a. Filaments closely compacted, suddenly acuminate . flmiiatilis, 244 a. Filaments erect, awl-shaped caspitosa, 245 84. GLCEOTRICHIA. § Sheath irregularly constricted; variable in size and form, natans, 246 § " close, simple, not inflated nor constracted, subspherical, pisum, 247 85. RIVULARIA. a. Sheaths inconspicuous; filaments clustered, base about 7/< diam., echitiulata, 249 a. Sheaths distinct; filaments some apparently withered, others more swollen, base 8-9/* diam. . dura, 249 64 SPECIES OF ALG^. 86 SCYTONEMA. § On warm sandy soil; blackish; diam. with sheath io-i4yu, thermak, 259 § On bark of trees, in small, olive green or brown tufts («). § On old wet wood {b). § On moist rocks, wall, earth, etc. {c). § Submerged in sweet water, [g). § Floating in sweet water; diam. of filaments 12-20/^ . . natans, 251 § " " " " " " " i4-i8yu cincinnatum, 254 § Brackish or salt; filaments curved and interwoven, f>4ryj-^<:,4/(?r«»?, 253 a. Filaments bluish; branches forming long loops, ends continuous, mirabilis, 255 a. Filaments creeping; branches not looped, ends not continuous, cortex, 256 b. Diameter of filaments with sheath about 2o/< . . intertextuni, 258 b. " " " " " " io-15/f . . simplice, 259 b. " " " " " " 20-25/^ . . Heppii, 260 c. More or less cushion-like, may become confluent (d). c. Forming a stratum {e). c. Tufted or turflike (/). d. On pots etc. in conservatory; filaments bluish, sheath close, color- less . .... Castellii, 255 d. On moist rocks, etc; sheath thick, often with a lime deposit, cinereum, 258 e. Diameter of sheath 25-33/1, lamellate .... myochrous, 252 e. Filaments flexuous, long, apices rounded; diam. i2-i6-25/(, cataracta, 252 e. " entangled, branches mostly single; cell ends somewhat contracted ... gracile, 253 e. Filaments interwoven, cells about as long as wide . turfosum, 253 e. " " sheath dissolving into fibrils, Notarissii, 255 /. Branches thianer, often JS4 or less than the st&m,tolypotrichoides, 250 f. Branches thinner at base only, quickly enlarging . Ncegelii 252 g. Filaments somewhat curved, branches thinner, bluish, calotrichoides, 251 87 SYMPHYOSIPHON. §. Diameter of filament without sheath 7/^ or less (a). §. " " " " " more than 7// {b). SPECIES OF ALG/E. 65 a. Diameter of filaments 2-3//, of sheath at the base 6-7/^, apex 3-4// crustaceus, 263 a. " " " 5-7/^ . ambiguum, 262 b. Surface pubescent; black; filaments 9-15/* . . Castelli, 262 b. " " filaments i5-20yu . Aiistinii, 262 (5. Surface not pubescent (^). c. Diameter of filaments 15/i or less {d). d. Filaments brown, 12-15/Y, cells j^ as long as wide, Bornetianum, 261 d. " bluish, 8-io/A, cells about as long as wide hirtubis, 261 d. " bluish, 8-12/^, sheath wider at base Hofmanni, 262 88. TOLYPOTHRIX. § On tree trunks, surface velvety, blackish; diam. ii-i4yM, trimcicola, 266 § On wet exposed rocks {a). § Aquatic (U). a. Reddish brown; filaments with dark transverse bands, Ravenellii, 265 a. Red, purple and black; filaments much branched . rupestris, 265 b. Spherical, pea-like masses, filaments bluish , yEgagrophila, 264 b. Not spherical masses (c). c. Diameter of filaments 3-4/U . . . . . , tenuis, 265, c. Diameter 10-17/^ (d). c. " 9-11;^, often interrupted by inclined disc-like cells, muscicola, 264 d. Impossible to separate here, distorta, 26y, ptikhra, 26^; flaccida, 265 89. PLECTONEMA. One species . . . . mirabile, 266 90. PETALONE.MA. One species . . . alatiun^ 267 91. SIROSIPHON. § Aquatic; brown; cells of stems 2-rowed, in branches i-rowed, ocellatus, 272 § On old wood and tree trunks; in black spots; often aggregated, lignicola, 273 § In soda springs, west; not fully described . Brandegeei, 274 § On damp soil or wet rocks (a). 66 SPECIES OF ALG^. a. Branches polymorphous (6). a. Branches not polymorphous (d). b. Apices obtusely rounded (c). b. " ' attenuate, or .filaments with scattered spines, Alpinus, 269 c. Plants attached only by the mucus surrounding them, pulvinatus, 268 c. Plants firmly attached to rocks and stones coralloides, 270 d. Diameter of filaments about 50//. Alpine Crameri, 273 d. " " " less than 50/^ (e). e. Cells broadly ovate, moniliform; on wet rocks compactus, 271 e. Cells small, not moniliform (y^). /. Filaments flexuous, curved, not rigid, branched, i6-i7yu, scytonematoides, 271 /. " short, intricate; branches many, irregular {g). g. Mature sheath deep reddish brown, of branches yellowish, apices colorless ... . . . argillaceus, 274 g. Mature sheath colorless, hyaline, rarely light yellow crustaceus 274 92. HAPALOSIPHON. § Aquatic, often parasitic {a). § On tree trunks (c). § On wet rocks((^). a. Diameter of filaments about 15/^; much branched, bluish; sheath often yellowish brown ... . . Brebissonii, 276 a. Diameter of filaments less than 15/* {Ji). b. Cells about as long as wide; filaments io-i2yU . . Braunii, 275 b. Cells about as long as wide; filaments 7-8//, branches 3-4/*, tcnuissimus, 277 b. Cells equal to twice as long as wide; filaments 8-1 lyu fuscescens, 277 c. Filaments 20-25//, branches rather thinner . byssoideus, 276 d. Filaments apt to be solitary, cells moniliform, bluish, torulosus, 276 93. NOSTOC. § Fronds soft, often without definite form [A). § Fronds globose or nearly so, or discoid or linear, often irregular with age {B). A. Aquatic (a). B. Aquatic (c). B. Terrestrial {b). SPECIES OF ALG^. 67 a. Without mucilage; filaments in irregular masses, coiled, tenuissimum, 282 a. With .mucilage; joints equal, rather distant . . comminutum, 282 b. Soft, becoming gelatinous cushions (if). b. " " tongue-shaped, plane, irregularly expanded {i). b. With a firm coating (/). c. Fronds with a firm coating (^). d. Mature frond suborbicular, often perforated and expanded, commune, 283 d. " " linear, straight, long; filaments parallel, flagelliforme, 285 e. Globose thalli within a subspherical gelatinous mass, muscorum, 282 e. Globose, firm; filaments olive . . . . . Aumifusum, 282 e. Suborbicular, soft; filaments bluish green . . . calcicola, 283 /. Globose, firm, coating brownish; joints -subspherical, sphcericum, 283 /. " soft, sheath often colored and general mucus colorless, rupestre, 283 /. " not I mm in diam.; cells cylindrical, slightly constricted, macrosporum, 284 g. Cells of two kinds, unequal (K). g. Cells uniform, regular [t). h. One kind elongated, the other spherical; bluish . cceruleum, 284 i. Fronds globose, from 2 mm to size of a cherry . pruniforme, 284 /. " rounded or discoid, becoming hollow (/), / Fronds subglobose, warty, brownish green . . verrucosum, 284 /• Fronds suborbicular, erect, smooth, tenacious . . Alpinum, 285 94. ANAB^NA. § On tree trunks, near the water's edge, bluish . cupressaphila, 288 § In brackish ditches; stratum bluish .... oscillarioides, 288 § In sweet waters (a), a. Cells 3-4 yw in diameter [b). a. Cells 4-ii/« cerugineo-ccerulea, 311 J- In brackish or submarine waters (k). J- In sweet waters (/). k. Diameter 18-20/* Grateloupii, 316 k. 12-15/* . . . littoralis, 317 SPECIES OF ALG^. /. Diameter 30yU or more (m). " less than 30// (n). m. Cells :^ to ^ as long as wide; diam. 30-45// m. Cells about \ as long as wide . n. Diameter less than 19/t (0). n. " ipyu or more (/). o. Diameter 15/i; cells /^-/^ as long as wide 0. " io-i8/<; variable /. Diameter 19-22//; cells Yi-yi as long as wide p. " ,23-28/i; cells |-|- " " " " . 71 princeps, 2>'^1 imperator, 317 . per cur sa, 315 Frxlichii, 315 major, 316 Bonnemasotiit, 316 105. BEGGIATOA. «. Diameter of filaments 1-1.5/i «. " " " 1. 8-2.5// niveicm, 318 leptom itiformis, 319 106. LEPTOTHRIX. ^. In hot waste water, waving, 6 mm thick hinmilia, 320 On old wet walls; filaments strongly curved . calcicola, 319 On wet wood, herbaceous green; diam. 1.8-2.25// herbacea, 320 Aquatic, sometimes on wet rocks {a). Forming spherical masses, light yellowish, tough, hollow, 4-8 mm diam . . ... bullosa, 321 Forming cloud-like, floating fragile masses, yellowish; filaments about 2// diam. . . ochracea, 322 Forming a stratum (V). Forming a tuft (e). Filaments 3// or more in diam. -. tenax, " less than 3// in diam. {/). Stratum composed of clusters, light bluish . rigidula, " more or less expanded [d). Bluish or greenish above, yellowish or colorless below, laminosa, Bright bluish, thin, membranaceous . ceruginea. Bluish, becoming yellowish . . ccespitosa, 320 Purple green or amethyst . . tinctoria, 321 319 321 321 319 I06fl. ASTEROTHRIX. One species Creginii, 322 72 SPECIES OF ALGM. 107. SPIRULINA. § A continuous, twisted, ring-like band . . duplex, 323 § Not continuous but filamentous {a). In stagnant water; diameter 7-8/x . . Jenneri, 323 a. In sulphur springs; diameter 4-5 }i tenuis sirna, 323 a 108. SPIRILLUM. One species . . . undula, 324 109. GLCEOTHECE. One species . . . confluens, 325 no. APHANOTHECE. a. More or less globose, size of a cherry, usually floating, • prasina, 325 a. Gelatinous, 4-6 mm thick, in siiiall masses; wet ground, pallida, 325 HI. SYNECHOCOCCU.S. One species; on wet rocks a.ruginosus, 326 112. MERISMOPEDIA. a. Rarely more than 4-64 cells, 3-5/* diam glauca, 326 a. Often of 256 geminate cells, 4-^.^fi diam. . convoluia, 326 113. CCELOSPH^RIUM. One species . Keutzingianum, ^2"] 114. CLATHROCYSTIS. One species . ... aruginosa, 327 115. GOMPHOSPH^RIA. One species . . . . . aponia, 328 116. MICROCYSTIS. One species; on moist timbers, ground, tree trunks, protogenita, 328 117. ANACYSTIS. a. Cells I /^ in diam.; thallus light brown . . . . brunnea, 329 a. Cells 2-3/< diam. " glaucous or pale bluish . pulvereus, 329 a. Cells 3-4/^ " " globular, margins colorless, marginata, 329 118. POLYCYSTIS. One species ... . icthyoblabe, 330 SPECIES OF ALG^. 73 Iig. GLOeOCAPSA, These forms have been divided into as many species as there are different colors to the cells or to the integument; they are green, red, black, brown, coppery, orange, yellow, olive, purple or colorless. They have no value as distinct plants, being phases or stages of de- velopment of higher Algae. Mr. Wolle considers them to be spores or macrogonidia, of Sirosiphon . 331 120. APHANOCAPSA. § Submerged; thallus globose . Grevillei, 333 § On wet rocks; thallus expanded virescens, 333 121. CHROOCOCCUS. a. Cell contents bright bluish, becoming brownish; 13-25// diam. turgidus, 334 a. " " reddish or yellowish brown; i2-i6/< diam. rwfescens, 335 a. " " blue green; 3-6// diam. cohxrens, 335 PART III. Key to the Genera and Species of the Desmidieae. A KEY TO THE GENERA AND THE SPECIES OF THE DESMIDIE^.* Plants composed of symmetrical cells, usually constricted in the middle, often of beautiful, ornamental forms, single or conjoined into filiform series, and sometimes involved in a maternal jelly. Asexual multiplication takes place by transverse division, or by sep- aration of the semi-cells, and by development of new parts similar to the mother semi-cells.** *The greater part of the following key was originally published in ' 'The American Monthly Microscopical Journal," and is here reproduced by permission of the editor. **Wolle: Desmids of the United States. KEY TO THE GENERA OF THE DESMIDS. § Cells united into filamefnts (a). § Cells not united into filaments (g). a. In a transparent, jelly-like sheath (b'). a. Not in a jelly-like sheath {d'). b. Cells with 2 teeth on each narrow end Desmidium, 6 b. Cells deeply constricted, almost into two parts (c). b. Cells not deeply constricted, and without teeth . . Hyalotheca, 3 c. With 'claspers' across the sutures ... . Onychonema, 9 c. Without 'claspers;' cells united by a narrow isthmus, Sphcsrozosma, 8 d. Band not twisted; cells with 'claspers' across the sutures, Onychonema, 9 d. " " cells without 'claspers' (e). d. Band twisted; cells triangular or quadrangular . Desmidium, 6 e. Cells barrel or hub-shaped, with i or 2 median bands, Bambusina, 4 e. " " " without bands, the sutures projecting, Leptozosma, 5 e. Cells cylindrical, sometimes swollen at base (/). e. Cells quadrangular, deeply constricted, often slightly twisted, Phymatodocis, 7 /. Ten to 30 times longer than broad . Gonatozygon, i /. Three to 6 times longer than broad . . . Genicularia, 2 g. Cell more or less crescentic . Closterium, 13 g. Cell cylinilrical, fusiform, dumb-bell or hour-glass shaped (/). g. Cell flattened; oiljiralar, oblong, or elliptical {K)'. h. Mostly orbiculai- or broadly elliptical; centre deeply constricted, the semi-celh 3-5 lobed, the lobes entire or variously incised, Micrasterias, 22 h. Mostly oblong or elliptical; margins wavy, the depressions rounded; ends usually notched or incised ... . Euastrum, 21 GENERA OF DESMIDS. 79 /. Cell constricted in the middle; no arms nor spines (/). i. " " " with arms or spines (/). /. Cell not constricted; no arms nor spines (ni). j. Cell cylindrical, ends simply notched .... Tetmemorus, i8 j. " " ends rounded, truncate or divided (k). j. Cell more or less dumb-bell, or hour-glass shaped {/). k. Cell 6 to 30 times longer than broad Docidiuui, 14 k. Cell 2-5 times longer than broad; ends rounded, Calocylindrus, 16 /. Arms 2, 3 or more, radiating . . . . . Staurastrum, 23 /. Arms none; semi-cells with a central, rounded, truncate or dentic- ulate tubercle; spines usually numerous and marginal, Xanthidium, 19 /. Arms none; no central tubercle; spines 4to 8, two on each end, ) /. " " " spines 16, four on each end, j Arthrodesmns, 20 m. Chlorophyll in one or more spiral bands . . . Spirotcenia, 11 m. " not in spiral bands (n). n. Surface rough with tooth-like or rounded elevations, Triploceras, 15 n. Surface without tooth-like elevations; ends rounded (0). 0. Cells in mucus, short, cylindrical or oval . . . Mesotmnium, 10 o. Cells not or rarely in mucus . . ... . Penium, 12 p. End view 3-6 or more angular (r). p. End view not angular (s). r. Angles obtuse, acute, or with horn-like prolongations, Staurastrum, 23 s. Margins smooth, dentate or crenate; no spines . Cos?nartum, 17 KEY TO THE SPECIES OF THE DESMIDS. I. GONATOZYGON. 1. Cells swollen at base, with 6 longitudinal lines of short seta, sex-spini/ei-um.'** 2. Cells not swollen at base {a). a. With hair-like spines clothing the surface- . . . pilosum, 22 a. Without hair-like spines; surface minutely roughened, asperum, 22 2. Genicularia.* Fig. 2. 1. Cells 3^ to 6 times longer than broad; granules in spirals, Americana* I. Cells 10-12 times longer than broad; granules scattered, spiroicenia* 3. Hyalotheca. Fig. 3. I. Cells slightly constricted, length }4 the width, sides not notched, dissiliens, 2 2 1. " " " " " sides arched, cen- trally notched . hians.** 2. Cells slightly concave, length twice the width . undulata, 23 3. Cells not co.istricted, margins straight; sheath wide, mucosa, 23 3. " " " " sheath absent, duhia, 24 4. Bambusina. Fig. 4. 1. Cells hub-shaped, somewhat longer than broad Brebissonii, 24 2. Cells hub-shaped, nearly twice as long as broad gracilescens.** 3. Cells subcylindrical, 4 times longer than broad ddicatissinia, 25 *Journ. R. Micr. Soc, Dec, 1885, **F. W. Alg£E, p. 21. SPECIES OF DESMIDS. 8l 5. Leptozosma. An immature form of Desmidium, 6. 6. Desmidium. Fig. 6. I. Mucous sheath present . . I. Mucous sheath absent (a), a. Cells united by their entire end margins {b). a. Cells united by the outer portions of the ends (errucosmn, 52 /. " with 20 or more constrictions . . cosiatitm, 5 3 /. " with I inflation (^). *F.-w. Algs, p. 25. 86 SPECIES OF DESMIDS. g. End with numerous pearly teeth or beads . . coronulatum, 49 g. End with prominent teeth, about 3 in view . . tridentulnm, 52 h. End with 3-5 minute tubercles; semi-cell with 4 or more undu- lations . . . . ... ... Floridense, 159 h. End with toothed angles (i). i. Semi-cell with 4 prominent nodes; 8 to 10 times longer than wide, nodosum, 50 /. " " 4 constrictions; 10-12 times longer than wide, breve, 51 /. " " 8 constrictions; 20-24 times longer than wide, sinuosum, 51 J. Semi-cell with i basal inflation [k). J. " undulate to near the end (/). /. " with 4 larger and smaller undulations; cells 12 times as long as wide (m). j. Semi-cells not or slightly undulate; densely granulate, breve, 158 k. Cytioderm densely, irregularly punctate .... clavatum, 48 k. " smooth; ends truncately rounded, margins often wavy, Baculum, 49; rectum* k. " " ends round; cell minute; diam. "j-izjx, ?mnutum, 52 k. " " " " cell, large; diam. ends soyU, Woodii* I. Diameter y^^ in. (25/i); about 20 times longer than wide, repandum, 50 /. Diameter yjVs" ^o t"sVb ''^- (13-1^/^); 15-20 times longer than wide, dilatatum, 50 /. Diameter ■j^Vb' '^^ fitW 'n. (io-i2/<); 18-20 times longer than wide, undulatum, 51 m. Surface punctate; suture none; ends roundly truncate, Georgicum* 15. TRIPLOCERAS. FIG. 15. (Mr. Wolle unites this with Docidium, 14.) I. Tooth-like prominences oblong verticillatu77i, 53 5. " acute . . . ... . . gracile, 53 16. CALOCYLINDRUS. FIG. l6. § Chlorophyll homogeneous (i). § " divided or scattered in each semi-cell (2). *F.-w. AlgEe, p. 25 et seq. SPECIES OF DESMIDS. 87 I. Length twice the width or less; cytioderm punctate or granulate {a). I. " " " " " " cytioderm smooth (i^). I. " " " " " " cytioderm with 5-7 costae, costatus, 5 6 I. Length zj^ or 3 times the width; cytioderm punctate (c). 1. " 4 to 6 times the width; cytioderm smooth . niinutus, 54 2. Cell twice or more longer than wide; cytioderm punctate, pseudoconnatus, 55 2. " " " " " " cytioderm smooth, Thwaitsii, 5 6 a. Semi-cell subquadrate ... Ralfsii, 54 a. " cylindrical, rounded; constriction slight . cuciirbita, 54 a. " " constriction wide, shallow . . connatus, 55 a. " sub-semicircular; constriction slight; ends rounded or somewhat truncate; end view circular . . . Cordanum, * b. Cell somewhat fusiform, ends subconically rounded . curtus, 54 b. Cell subcylindrical, ends broadly rounded . diplospora, 56 c. Cell subcylindrical, ends rounded; nuclei large, single or double, Clevei, 56 17. COSMARIUM. FIG. 17. § End view without central inflations (i). § End view with central inflations (2). I. Cytioderm smooth or punctate (a). I. " more or less verrucose or granular (/). 1. " spinous {g). 2. Cytioderm smooth or punctate {h). 2. " more or less verrucose or granular (z). a. Chlorophyll diffused (b). a. Chlorophyll concentrated in i or more nuclei (e). b. Margins crenate, undulate, dentate or granulate (c). b. " not crenate, undulate, dentate nor granulate (d). c. Margin crenate (s). c. " undulate (/). c. " granulate (u). e. " dentate or notched; end truncafe, smooth; sides toothed, convex ... . . . Reinschii, 68 e. Margin dentate; end 4-spined; sides smooth, convex, aculeatum, 66 d. Cell twice or more longer than wide (/). d. Cell i^ or less than twice longer than wide [k). d. Cells less than i^ times longer than wide (/«). *F.-\v. AlgEE, p. 27. 88 SPECIES OF DESMIDS. ^. Margins crenate, undulate, dentate or granulate (f). e. Margins smooth; basal angles not toothed (w). e. Margins smooth; basal angles each with i, tooth or papilla, Smolandicum, 69 /. Verrucae or granules in patterns, or more or less restricted {x). f. " " " not in patterns; more or less covering the surface (cc). g. Spines on the whole surface; cell suborbicular; sinus elliptical, aculeatum, 66 g. Spines marginal, conical; centre with 7 granules, and punctate, trachypleurum^ 73 h. Margins crenate, undulate, or dentate {jj). h. Margins smooth (//). /. Verrucse or granules in patterns, or more or less restricted {nii). i. " " " not in patterns, more or less covering the surface (vv). j. End rounded; semi-cell oblong, sides almost parallel, De Baryi, 58 j. " " semi-cell spherical, . . moniliforme, 60 j. '■ " semi-cell elliptical or oval . corda?ium* oblongum\ j. End truncate; semi-ceil quadrate, sides almost parallel, anceps, 59 / " " semi-cell pyramidal . parvulum, 59 k. Cell joined by pellucid bands into families Quimbyii, 61 k. Cells not joined into f:Lmilies (/). /. Ends rounded; semi-tells oblong . . cucumis, 58 /. " " semi-cells oval; diameter y^ij^ in. (23//), Witrockii\ I. Ends truncate; semi-cells pyramidal, sides convex; diameter -^^ to y^ij^ (22-28/*) . . . notabile, 66 /. Ends truncate; semi-cells pyramidal, sides convex; diameter ^-^ to -g-J-Q (So-85/O ■ • ■ • py7'amidatmn, 69 /. Ends truncate; semi-cells pyramidal, sides convex ; diameter y^ij^' to -j-J^ (25-43yu) . . pseudopyramidatum, 69 /. Ends truncate; semi-cells pyramidal; sides straight or slightly concave, . . . . .... . .granaium, 60 m. Cell conspicuously broader than long; semi-cells subsemicircular; side view circular, end view elliptical; diameter 32-38/*, scenedesmus, 59 m. Cell conspicuously broader than long; semi-cells subsemicircular; side view ovoid; diameter 75-85/* . . circulare.** * Journ. R. Micr. Soc, Dec, 1885. t Journ. R. Micr. Soc, Feb., i886. ** F. W. Algse, p. 27 et seq. SPECIES OF UESMIDS. 89 //;. Cell nearly as long as broad; semi-cells rhomboid, sides all equal; sinus deep, wide .... . rhombusoides.** n. Ends rounded; semi-cell subcircular, sinus a mere notch, globostim, 60 n. " " semi-cell subsemicircular (\; orthosticum, 78 gg. •'■ semi-orbicular, tetraophthalmum, 75 ; intermedium, 75 hh. Semi-cells pyramidal, end truncate; basal angles rounded, ^ octkodes, 76; Botrytis, 74 hh. Semi-cells oval or elliptical, approximate . . . trigonum*' margaritiferum, 'jc^; punctulatum, 74 hh. " " " remote, granulate, portiannm, 77 hh. Semi-cells quadrangular, ends truncate; diam. -5'^ --gi^ (60-73/1),. conspersum, 75 hh. " " ends concave . . . retusum.* hh. " " diam. ^-^(30-45 /<), pseudobroomei, 86 hh. " " diam. ^^-^^15^(13-14/^), sphalerostichum. * hh. Semi-cells subreniform, sinus widened and rounded inwardly, latum, 76; renifoi-me, 76; pardalis.* hh. Semi-cells subspherical, approximate . . . orbiculatum, 77 hh. Semi-cells hemispherical, remote; base flattened, excavatum, 77 a. Ends and sides with teeth; sinus narrow, widened outwardly; basal angles rounded . Brebissonii, 75 a. Ends without teeth, sides with 10 to 20; sinus gaping, dentatum, 76 jj. Margins crenate; semi-cell semicircular, nuclei 2, end truncate, cruciatum, 81 jj " undulate; sinus widening outwardly, ho7nalodermum, 81 jj. " " sinus not widening outwardly (M). ^k. Cell longer than wide; diameter -5-^ to -jp^ in. (38-44//), sublobatum, 80 kk. Cells longer than wide, diameter yuVir to Y^wa '"• (22-25//), mai-garitum, 80 kk. Cell not longer than wide, end truncate . . retusum, 80 //. Diameter -j-JL-j- in. (12//), end truncate; sides convex, often ob- tusely angled centrally . . . Schliephanckeanum, 82 //. Diameter greater than (i2/() -j-oVo '"• (fn''n). *F.-w. Algse, p. 25. SPECIES OF DESMIDS. 93 mm. Sinus acute inwardly .... . . thithophorum, 80 mm. " rounded and widened inwardly . . phaseolus, 8i mm. " rounded, but not widened inwardly, pseudogranatum., 158 nn. Margins crenate or dentate (00). nn. Margins smooth; centre with one verruca; semi-cell elliptical:, diameter ysVo'"- (''3y") • • '■ • • • bireme, 82. vo. Ends truncate; diametery-|^ in. (33/^) or larger {pp). 00. " " diameter -^ in. (28/() or smaller (7-r). 00. Ends rounded, dentate . . . . . . . Lagoense, * //. About i-|- times longer than wide; diameter 1^ to ^-^ in. (33-50/^) triplicatum ■]T,;speciosum, 87 pp. About \ longer than wide; diameter -^ to -j-^ in. (65-7oyu), supraspeciosum, 88 //. About \ longer than wide; diameter -^\-^ in. (soyu), pychnochondnim, 89 rr. Diameter -g^-j to yJjTr (2o-26/<); ends 4 crenate, sides 4-6 crenate, subcrenatum, 84. ''''• " TTiVo-to tAt (^°~^S^)' sides nearly straight, Kjdlmaiiii, 87 rr. " y^Vo to y-gVff (i4-2Sy");granulesnotradiate; end4crenate, Blyttii, 87 j-j-. Marginal teeth numerous, long, pointed or aculeate, Eloiseanum, 85 «. ,_ 17, emarginate-truncate . qiiadrifariiim, 87 jj. Margins crenate (#). /^. Basal inflation granulate in vertical lines {uii). tt. " " with scattered granules . pseudopectiiwides, 89 tt. " " without granules, the marginal in 8 radiating lines, nasKtinn, 89 uii. Sinus widening outwardly; granules geminate in rows, pectiiwides, 88 UII " not widening outwardly; cell oblong, diameter —W {TiZ), pulchej-ritni/m, 90 uii. " " " cell orbicular, diameter -jJ-^ (50/^), radiosiim, 90 vv. End truncate i^wiv). w. End not truncate {zz). • M/?e'. Diameter T^ in. (50/^) or larger (.r.v). ■a/w. Diameter smaller than (50/^), ^L- in. ( rr). *Journ. R. Micr. Soc, Dec, 1SS5. 94 SPECIES OF DESMIDS. XX. Sides granulate, concave near the ends; semi-cell twice longer than wide . . protr actum, 83 XX. Sides granulate, ends concave; semi-cell twice wider than long, biretum var. Floridense.^ XX. Sides crenate-undulate, converging; cytioderm verrucose, Quasillus, 84 XX. " rounded, acutely toothed, ends usually nude; cell as long as wide . . Everettense, 85 XX. " straight, diverging, verrucose; angles rounded, biretum, 86 yy. Cytioderm granulate; cell widest at base, sides converging, sportella, 83 yy. " " cell narrowed at base, sides straight, diverging, protuberans, 84 yy. Cytioderm verrucose; end more or less protruding and scolloped (4-crenate) ... . . . . . ccslatum, 86 yy. Cytioderm verrucose; end more or less protruding, not scolloped, ornatu7n, 82; protractum, 82 yy. " " end not protruding; semi-cell twice as long as wide, oblong-quadrangular, angles rounded, Broomei, 86 zz. Cytioderm finely granulate or punctate; semi-cells triangular, angles rounded, margins smooth . ... Turpinii, 158 zz. Cytioderm verrucose; semi-cell subreniform, 3 times as wide as long . . ... commisurale, 83 zz. " " semi-cell pyramidal, angles rounded, long, tumidum, 75 zz. " " semi-cell reniform, angles denticulate, a central inflation granulate . ... subcruciforme.* 18. Tetmemorus. Fig. 18. § Cytioderm smooth or very indistinctly punctate (c). § Cytioderm punctate {a). a. Cell 3 times as long as wide, irregularly granular; base slightly plicate. . gtganieus, '92 a. Cell more than 3 times as long as wide (b). b. Front and lateral views fusiform; end with colorless, lip-like pro- jection . ... . . ... granulatus, 91 b. Front view cylindrical, not tapering; side view fusiform, tapering; end rounded . . . Brebissonii, 91 * F. — w. Algae, p. 27 et seq. SPECIES OF DESMIDS. 95 c. Three times longer than wide, smooth; diameter y'tstt to x^rj- (18-20/^) minutus, 91 c. Four times longer than wide, smooth; diameter -j|^ (48/*) ; linear elliptical, no lip penioides* c. Four to six times longer than wide, smooth or indistinctly punc- tate; front view tapering, lateral fusiform, ^rsr '^Onrs" (20-22/*),. Icevis, 91 19. Xanthidium. Fig. 19. § Spines divided at the ends armatum, 92 § Spines subulate, ends not divided {a). a. Spines more or less scattered, numerous . . acicleatuin, 92 a. " " " " 4 within the margins, Columbtanunr** a. Spines marginal (f). b. Basal angles with 2 spines (a'). b. " " " I spine {c). c. Other spines geminate in 4 pairs ... . cristatum, 93 c. " " " in 2 pairs on the end, single on the sides,. astepticm, 93 c. " " not geminate, single on the ends . . Torreyi.*'^ d. Other spines 6 to 10 pairs on semi-cell; protuberance beaded, bisenariwn, 93 d. Other spines 2 to 4 pairs {e). d. " " none . . . tetracentrotum, 95 e. Other spines, 4 pairs, terminal ... . . fasciculatum, 93 e. " " 2 pairs, basal, vertical . . reciocornutum, 94 e. " " 2 pairs, terminal; a row of granules above the cen- tral projection, a spine above the granules, Minneapoliense, 94 e. Other spines, 2 pairs, terminal; a row of granules above the cen- tral projection, no spine above the granules . polymazum, 94 e. Other spines, 2 pairs, terminal, no granules above the projection (/)• /. Diameter ^^ in. (ss-65/<) or more, Tylenanum\, fasciculatum, 93 /. " -5^ in. (soyu) or less; semi-cell truncate-triangular, asteptum, 93 /. " " " semi-cell not truncate-triangular, aniilopcBum, 94 *Journ. R. Micr., Soc, Feb., 1886. **F. W. Algfe, p. 34 et seq. fjourn. R. Micr. Soc, Dec, 1885. 96 SPECIES OF DESMIDS. 20. Arthrodesmus. Fig. 20, a, b. I Cytioderm smooth {a). § Cytioderm verrucose or spinous (V). a. Semi-cell with 2 spines (c). a. Semi-cell with more than 2 spines (d'). b. Cytioderm with deciduous spines Rauii, 95 b. " verrucose in rows, margins crenate . guadridens, 96 i. " " " ends alone crenate, notochondrus.** c. Spines on the same side diverging (e). £. " " parallel {g). c. " " converging . . convergens, 95 d. Margin of semi-cell angdlar, each angle with i or two spines, octocornis, 97 e. End truncate; spines widely divergent . . . Incus, 97 e. End convex; spines moderately divergent (/). f. Diameter of cell -j^ (40/') • incrassatus* f. " " 8^ — tk (3°-3S/^) • • • subulatus, 96 /. " " -rh^ (2°/<) o^'alis, 96 g. Nuclei, 2 in each oblong-oval semi-cell . ... fragilis, 95 g. Nuclei none; semi-cell oval, diameter y-^^ in. (20/i) . ovalis, 96 g. " semi-cell orbicular, diameter -^-gij-jj- (i2/(), orbicularis 96 g, " semi-cell elliptical; spines often very short, convergens var., 95 g. " semi-cell triangular, sinus wide, isthmus cylindrical, triangtdaris, 36 ^^ 21. EUASTRUM. FIG. 21. § End lobe evidently distinct (a). § End lobe evidently not distinct (b). a. End lobe deeply notched {c). a. End lobe more or less concave or sometimes convex (^.) b. End deeply notched (e). b. End more or less convex; semi-cell with 7 or 8 lateral, short, coni- cal teeth ... . Donnellii, 103 b. End more or less convex; sides without teeth . pingue, 105 c. Margins smooth (^ . . Special estimates made for any required quantity for class use. MAGNIFIERS $1 50 Pocket Magnifiers, Coddingtons, Achro- matic Triplets and Doublets. Prices from 25 cents to $5.00. N. Y. OFFICE, SEND FOR BOTANICAL AND ENTOMOLOQICAL CIRCULAR B. B. QUEEN & CO., Inc. loio Chestnut Street .WllIIIIIMIl"""* PHILADELPHIA